| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 3,544,446 | A→C | 15.8% | S133R (AGT→AGG) | bioH ← | pimeloyl‑ACP methyl ester carboxylesterase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,544,446 | 0 | A | C | 15.8% | 191.6 / 3.6 | 79 | S133R (AGT→AGG) | bioH | pimeloyl‑ACP methyl ester carboxylesterase |
| Reads supporting (aligned to +/- strand): ref base A (51/12); new base C (6/10); total (57/22) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.24e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ACCAGACCGTCGAGATAGCCATACAATCGCAAAAACGGCATGGACACGTTTTGCAGCGGCTGACGGAGATCGACCGTTTTCAGGATTTCCAGCCCGCCATTAAGCACGTCAACCTCCGGCATCGGTAACGCCAGAACGGTTTTCTTCAACGCCCGCGCATCCTGGCGCGCCGTTTCAGTCCCCATGGTTTGTAACGCCAGGAACCGCTCCACTGTACGCTGAAAATCATCACTGAGTTGCTGCTGAAATCCCGCCAGCACGTCCGGTTTTATCCCCGGCCACTCGTCACGAGCACTAAAACAAGGTGACGACGCCACGGTGACCAGCG‑CCTGAACACGCTCGGGATGGGTTAACGCAATCTGGCTTGCCACCAGCCCGCCCAGACTCCAGCCTAACCAAATGGCTTTATCAGGTGCCTGTTGCAGCACGGCTTCGGCCATATCAGCAAGTGACAGCGCACCAAA > NC_000913/3544216‑3544679 | aCCAGACCGTCGAGATAGCCATACAATCGCAAAAACGGCATGGACACGTTTTGCAGCGGCTGACGGAGATCGACCGTTTTCAGGATTTCCAGCCCGCCATTAAGCACGTCAACCTCCGGCATCGGTAACGCCAGAACGGTTTTCTTCAACGCCCGCGCATCCTGGCGCGCCGTTTCAGTCCCCACGGTTTGTAACGCCAGGAACCGCTCCACCGTACCCTGAAGATCATCCCTGGGtt > 1:1075428/1‑238 (MQ=255) gACCGTCGAGATAGCCATACAATCGCAAAAACGGCATGGACACGTTTTGCAGCGGCTGACGGAGATCGACCGTTTTCAGGATTTCCAGCCCGCCATTAAGCACGTCAACCTCCGGCATCGGTAACGCCAGAACGGTTTTCTTCAACGCCCGCGCATCCTGGCGCGCCGTTTCAGTCCCCCGTGTTTGTAACGCCCGGAACCCCTCCCCTGTACGCCGAAAACCCTTCCCGAGTtgcgg > 2:394534/1‑236 (MQ=255) tCGAGATAGCCATACAATCGCAAAAACGGCATGGACACGTTTTGCAGCGGCTGACGGAGATCGACCGTTTTCAGGGTTTCCAGCCCGCCATTAAGCACGTCAACCTCCGGCATCCGTAACGCCAAAACGGTTTTCTTCAAGCCCCGCCCATCCTGTCGCGCCGTTTCAATCCCCATGGGTTTGAACGCCAGGAACCCCTCCACCTTACGCTGAAAATCACCAATAGGTtgccgcgaaaa > 2:1072821/1‑234 (MQ=255) tCGAGATAGCCATACAATCGCAAAAACGGCATGGACACGTTTTGCAGCGGCTGACGGAGATCGACCGTTTTCAGGATTTCCAGCCCGCCATTAAGCACGTCAACCTCCGGCATCGGTAACGCCAGAACGGTTTTCTTCAACGCCCGCGCATCCTGGCGCGCCGTTTCAGTCCCCATGGTTTGTAACGCCAGGAACCGCTCCACTGTCCGCTGAAAATCATCACTGAGTTGCTGCCGaaa > 1:728983/1‑239 (MQ=255) tCGAGATAGCCATACAATCGCAAAAACGGCATGGACACGTTTTGCAGCGGCTGACGGAGATCGACCGTTTTCAGGATTTCCAGCCCGCCATTAAGCACGTCAACCTCCGGCATCGGTAACGCCAGAACGGTTTTCTTCAACGCCCGCGCATCCTGGCGCGCCGTTTCAGTCCCCATGGTTTGTAACGCCAGGAACCGCTCCACTGTACGCTGCAAATCCTCCCTGAGTTGCTGCTGaa > 1:805970/1‑238 (MQ=255) tCGAGATAGCCATACAATCGCAAAAACGGCATGGACACGTTTTGCAGCGGCTGACGGAGATCGACCGTTTTCAGGATTTCCAGCCCGCCATTAAGCACGTCAACCTCCGGCATCGGTAACGCCAGAACGGTTTTCTTCAACGCCCGCGCATCCTGGCGCGCCGTTTCAGTCCCCATGGTTTGTAACGCCAGGAACCGCTCCACGGTACGCTGAAAAACATCACTGGATTGCTGCTGaa > 1:390601/1‑238 (MQ=255) tCGAGATAGCCATACAATCGCAAAAACGGCATGGACACGTTTTGCAGCGGCTGACGGAGATCGACCGTTTTCAGGATTTCCAGCCCGCCATTAAGCACGTCAACCTCCGGCATCGGTAACGCCAGAACGGTTTTCTTCAACGCCCGCCCATCCTGGCGCGCCGTTTCAGTCCCCATGGTTTGTAACGCCCGGAACCGCCCCCCTGTACACCGAAAACCATCACTGAGTTGGCGCTGaaa > 2:909255/1‑239 (MQ=255) ccATACAATCGCAAAAACGGCATGGACACGTTTTGCAGCGGCTGACGGAGATCGACCGTTTTCAGGATTTCCAGCCCGCCATTAAGCACGTCAACCTCCGGCATCGGTAACGCCAGAACGGTTTTCTTCAACCCCCCCCCATCCTGGCCCGCCGGTTCCGTCCCCATGGTTTGTAAACCCAGGAACCCCCCCACCTTACCCCGAAAACCATCACTGAGTTGCCGCTCAAAAccccccca > 2:954732/1‑237 (MQ=255) tCGCAAAAACGGCATGGACACGTTTTGCAGCGGCTGACGGAGATCGACCGTTTTCAGGATTTCCAGCCCGCCATTAAGCACGTCAACCTCCGGCATCGGTAACGCCAGAACGGCTTTCTTCAACGCCCGCGCATCCTGGCGCGCCGTTTCAGTCCCCATGGTTTGTAACGCCAGGAACCGCTCCCCTGTACGCTGCAAATCATCCCTGAGTTGCTCCTGAAATCCCCCCCCCACGTcgg > 2:849682/1‑237 (MQ=255) cAAAAACGGCATGGACACGTTTTGCAGCGGCTGACGGAGATCGACCGTTTTCAGGATTTCCAGCCCGCCATTAAGCACGTCAACCTCCGGCATCGGTAACGCCAGAACGGTTTTCTTCAACGCCCGCGCATCCTGGCGCGCCGTTTCAGTCCCCATGGTTTGTAACGCCAGGAACCGCTCCACTGTACGCTGAAAATCATCACTGAGTTGCTGCTGAAATCCCGCCCGCACGTCCGGtt > 1:33724/1‑239 (MQ=255) cAAAAACGGCATGGACACGTTTTGCAGCGGCTGACGGAGATCGACCGTTTTCAGGATTTCCAGCCCGCCATTAAGCACGTCAACCTCCGGCATCGGTAACGCCAGAACGGTTTTCTTCAACGCCCGCCCATCCTGGCGCGCCGTTTCAGTCCCCATGGTTTGTAACGCCAGGAACCGCTCCACTGTACGCTGCAAATCATCCCTGAGTTGCTGCTGAAATCCCCCCACCACCTCCGGtt > 2:125793/1‑239 (MQ=255) gACACGTTTTGCAGGGGCTGACGGAGATCGCCGGTTTGCAGGTTTTCCAGCCCGCCATTAAGCACGTCAACCTCGGGCATCGGTAACGCCAGAACGGTTTTCTTCAACGCCCGCGCATCCTGGCGCGCCGTTTCAGTCCCCATGGTTTGTAACGCCAGGAACCGCTCCACTGTACGCTGAAAATCATCACTGAGTTGCTGCTGAAATCCCGCCAGCACGTCCGGTTTTATCCCCGGc < 1:484069/237‑1 (MQ=255) ttttGCAGCGGCTGACGGAGATCGACCGTTTTCAGGATTTCCAGCCCGCCATTAAGCACGTCAACCTCCGGCATCGGTAACGCCAGAACGGTTTTCTTCAACTCCCGCGCATCCTGGCGCGCCGTTTCAGTCCCCATGGTTTGTAACGCCAGGAACCGCTCCACTGTACGCTGAAAATCATCACTGAGTTGCTGCTGAAATCCCGCCAGCACGCCCGGGTTTATCCCCGGCCAcccccc > 2:716066/1‑234 (MQ=255) cAGCGGCTGACGGAGATCGACCGTTTTCAGGATTTCCAGCCCGCCATTAAGCACGTCAACCTCCGGCATCGGTAACGCCAGAACGGTTTTCTTCAACGCCCGCGCATCCTGGCGCGCCGTTTCAGTCCCCATGGTTTGTAACGCCCGGAACCGCTCCACTGCACGCTGAAAATCATCCCTGAGTTGCTGCCGAAATCCCGCCACCACGCCCGGTTTTATCCCCGCCCACTCGTCACGAg > 2:637711/1‑239 (MQ=255) ggCTGACGGAGATCGACCGTTTTCAGGATTTCCAGCCCGCCATTAAGCACGTCAACCTCCGGCATCGGTAACGCCAGAACGGTTTTCTTCAACGCCCGCGCATCCTGGCGCGCCGTTTCAGTCCCCATGGTTTGTAACGCCAGGAACCGCTCCACTGTACGCTGAAAATCATCACTGAGTTGCTGCTGAAATCCCGCCAGCACGTCCGGTTTTATCCCCGGCCACTCGTCACGGGCACt > 1:343583/1‑239 (MQ=255) cTGACGGAGATCGACCGTTTTCAGGATTTCCAGCCCGCCATTAAGCACGTCAACCTCCGGCATCGGTAACGCCAGAACGGTTTTCTTCAACGCCCGCGCATCCTGGCGCGCCGTTTCAGTCCCCATGGTTTGTAACGCCAGGAACCGCTCCACTGTACGCTGAAAATCATCACTGAGTTGCTGCTGAAATCCCGCCAGCACGTCCGGTTTTATCCCCGGCCCCTCGTCCCGAGCACtca > 1:478543/1‑237 (MQ=255) gggAGATCGCCCTTTTTCAGGATTCCCGCCCCGCCATTAAGCCCGTCAACCTCCGGCCTCGGTACCGCCAGAACGGTTTTCTTCAACGCCCGCGCATCCTGGCGCGCCGTTTCAGTCCCCATGGTTTGTAACGCCAGGAACCGCTCCACTGTACGCTGAAAATCATCACTGAGTTGCTGCTGAAATCCCGCCAGCACGTCCGGTTTTATCCCCGGCCACTCGTCACGAGCACTAAAAc < 1:752278/237‑1 (MQ=255) aTCGACCGTTTTCAGGATTTCCAGCCCGCCATTAAGCACGTCAACCTCCGGCATCGGTAACGCCAGAACGGTTTTCTTCAACGCCCGCGCATCCTGGCGCGCCGTTTCAGTCCCCATGGTTTGTAACGCCAGGAACCGCTCCACTGTACGCTGAAAATCATCACTGAGTTGCTGCTGAAATCCCGCCAGCACGTCCGGTTTTATCCCCGGCCCCTCGTCACGAGCACAAAAAAAAAGTg > 2:138676/1‑239 (MQ=255) aCCGTTTTCAGGATTTCCAGCCCGCCATTAAGCACGTCAACCTCCGGCATCGGTAACGCCAGAACGGTTTTCTTCAACGCCCGCGCATCCTGGCGCGCCGTTTCAGTCCCCATGGTTTGTAACGCCAGGAACCGCTCCACTGTACGCTGAAAATCATCACTGAGTTGCTGCTGAAATCCCGCCAGCACGTCCGGTTTTATCCCCGGCCACTCGTCACGAGCACTAAAACAAGGTgacg > 1:100579/1‑238 (MQ=255) aCCGTTTTCAGGATTTCCAGCCCGCCATTAAGCACGTCAACCTCCGGCATCGGTAACGCCAGAACGGTTTTCTTCAACACCCGCGCATCCTGGCGCGCCGTTTCAGTCCCCATGGTTTGTAACGCCAGGAACCGCTCCACTGGACGCTGAAAATCATCACTGAGTTGCTGCTGCAATCCCGCCAGCACGTCCGGTTTTATCCCCGTCCACTCGTCACGCGCACTAAAACCAGGTgacc > 1:988079/1‑237 (MQ=255) ttCCAGCCCGCCATTAAGCACGTCAACCTCCGGCATCGGTAACGCCAGAACGGTTTTCTTCAACGCCCGCGCATCCTGGCGCGCCGTTTCAGTCCCCATGGTTTGTAACGCCAGGAACCGCTCCACTGTACGCTGAAAATCATCACTGAGTTGCTGCTGAAATCCCGCCAGCACGTCCGGTTTTATCCCCGGCCACTCGTCACGAGCACTAAAACAAGAGGACGACTCCCCGGTGAcc > 1:498779/1‑238 (MQ=255) tcccgccccGCCATTAAGCACGTCAACCTCCGGCATCGGTACCGCCAGAACGGTTTTCTTCAACGCCCGCGCATCCTGGCGCGCCGTTTCAGTCCCCATGGTTTGTAACGCCAGGAACCGCTCCACTGTACGCTGAAAATCATCACTGAGTTGCTGCTGAAATCCCGCCAGCACGTCCGGTTTTATCCCCGGCCACTCGTCACGAGCACTAAAACAAGGTGACGACGCCACGGTGAcc < 1:394534/232‑1 (MQ=255) tCCAGCCCGCCATTAAGCACGTCAACCTCCGGCATCGGTAACGCCAGAACGGTTTTCTTCAACGCCCGCGCATCCTGGCGCGCCGTTTCAGTCCCCATGGTTTGTAACGCCAGGAACCGCTCCACTGTACGCTGAAAATCATCACTGAGTTGCTGCTGAAATCCCCCCAGCCCGCCCGGTTTTATCCCCGCCCACTCCTCCCGAGCCCTAAACCAAGGGGACGACGCCCCggcgcccc > 2:588421/1‑232 (MQ=255) gCCATTAAGCACGTACACCCCCGGCTCCGGTAACGCCAGAACGGTTTTCTTCAACGCCCGCGCACCCTGGCGCGCCGTTTCAGTCCCCATGGTTTGTAACGCCAGGAACCGCTCCACTGTACGCTGAAAATCATCACTGAGTTGCTGCTGAAATCCCGCCAGCACGTCCGGTTTTATCCCCGGCCACTCGTCACGAGCACTAAAACAAGGTGACGACGCCACGGTGACCAGCG‑CCTGaa < 1:804623/239‑1 (MQ=255) ccATTAAGCCCGTCAACATCCGGCATCGGTAACGCCAGAACGGTTTTCTTCAACGCCCGCGCATCCTGGCGCGCCGTTTCAGTCCCCATGGTTTGTAACGCCAGGAACCGCTCCACTGTACGCTGAAAATCATCACTGAGTTGCTGCTGAAATCCCGCCAGCACGTCCGGTTTTATCCCCGGCCACTCGTCCCGACACCTCAAACAAGGTGCCGAGGCCCCGGGGACCacgcccggaaa > 2:878341/1‑229 (MQ=255) ccATTAAGCACGTCAACCTCCGGCATCGGTAACGCCAGAACGGTTTTCTTCAACGCCCGCGCATCCTGGCGCGCCGTTTCAGTCCCCATGGTTTGTAACGCCAGGAACCGCTCCACTGTACGCTGAAAATCATCACTGAGTTGCTGCTGAAATACCGCCAGCACGTCCGGTTTTATCCCCGGCAACTCGTCACGAGCACTAAAACAAGGTGACGACGCCACGGTGACCAGCG‑CCTGaaa > 1:930776/1‑238 (MQ=255) aaGCCCGTCAACCCCCGGCATCGGTAACGCCAGAACGGTTTTCTTCAACGCCCGCGCATCCTGGCGCGCCGTTTCAGTCCCCATGGTTTGTAACGCCAGTAACCGCTCCACTGTACGCTGAAAATCATCACTGAGTTGCTGCTGAAATCCCGCCAGCACGTCCGGTTTTATCCCCGGCCACTCGTCACGAGCACTAAAACAAGGTGACGACGCCACGGTGACCAGCG‑CCTGAACACGCt < 1:738930/239‑1 (MQ=255) gTCAACCTCCGGCATCGGTAACGCCAGAACGGTTTTCTTCAACGCCCGCGCATCCTGGCGCGCCGTTTCAGTCCCCATGGTTTGTAACGCCAGGAACCGCTCCACTGTACGCTGAAAATCATCACTGAGTTGCTGCTGAAATCCCGCCAGCACGTCCGGTTTTATCCCCGGc < 2:674476/172‑1 (MQ=255) gTCAACCTCCGGCATCGGTAACGCCAGAACGGTTTTCTTCAACGCCCGCGCATCCTGGCGCGCCGTTTCAGTCCCCATGGTTTGTAACGCCAGGAACCGCTCCACTGTACGCTGAAAATCATCACTGAGTTGCTGCTGAAATCCCGCCAGCACGTCCGGTTTTATCCCCGGc > 1:674476/1‑172 (MQ=255) gTCAACCTCCGGCATCGGTAACGCCAGAACGGTTTTCTTCAACGCCCGCGCATCCTGGCGCGCCGTTTCAGTCCCCATGGTTTGTAACGCCAGGAACCGCTCCACTGTACGCTGAAAATCATCACTGAGTTGCTGCTGAAATCCCGCCAGCACGTCCGGTTTTATCCCCGGCCCCTCGTCACGAACACTAAAACAAGGTGACGACCCCACGGGGACAAGCG‑CCTGAACAACCCCGGGaa > 2:62885/1‑238 (MQ=255) gTCAACCTCCGGCATCGGTAACGCCAGAACGGTTTTCTTCAACGCCCGCGCATCCTGGCGCGCCGTTTCAGTCCCCATGGTTTGTAACGCCAGGAACCGCTCCACTGTACGCTGAAAATCATCACTGAGTTGCTGCTGAAATCCCGCCAGCACGTCCGGTTTTATCCCCGGCCACTCGTCACGAGCGCTAAAACAAGGGGACGACGCCACGGAGACCAGCG‑CCTGAacaagaaccggat > 1:516572/1‑229 (MQ=255) gTCAACCTCCGGCATCGGTAACGCCAGAACGGTTTTCTTCAACGCCCGCGCATCCTGGCGCGCCGTTTCAGTCCCCATGGTTTGTAACGCCAGGAACCGCTCCACTGTACGCTGAAAATCATCACTGAGTTGCTGCTGAAATCCCGCCAGCACGTCCGGTTTTATCCCCGGCCACTCGTCACGAGCACTCAAACAAGGTGCCGACCCCCCGGGGACCAGCG‑CCTGAACACCCTCGGGag > 2:779330/1‑238 (MQ=255) gTCAACCTCCGGCATCGGTAACGCCAGAACGGTTTTCTTCAACGCCCGCGCATCCTGGCGCGCCGTTTCAGTCCCCATGGTTTGTAACGCCAGGAACCGCTCCACTGTACGCTGAAAATCATCACTGAGTTGCTGCTGAAATCCCGCCAGCACGTCCGGTTTTATCCCCGGCCACTCGTCACGAGCACTAAAACAAGGTGACGACGCCACGGTGACCAGCG‑CCTGAACACGCTTCGGAt > 1:540712/1‑239 (MQ=255) tCCGGCATCGGTAACGCCAGAACGGTTTTCTTCAACGCCCGCGCATCCTGGCGCGCCGTTTCAGTCCCCATGGTTTGTAACGCCAGGAACCGCTCCACTGTACGCTGAAAATCATCACTGAGTTGCTGCTGAAATCCCGCCAGCACGTCCGGTTTTATCCCCGGCCACTCGTCACGAGCACTAAAACAAGGTGACGACGCCACGGTGACCAGCG‑CCTGAACACGCTCGGGATGGGTTa > 1:13394/1‑238 (MQ=255) ggCATCGGTAACGCCAGAACGGTTTTCTTCAACGCCCGCGCATCCTGGCGCGCCGTTTCAGTCCCCATGGTTTGTAACGCCAGGAACCGCTCCACTGTACGCTGAAAATCATCACTGAGTTGCTGCTGAAATCCCGCCAGCACGTCCGGTTTTATCCCCGGCCACTCGTCACGAGCACTAAAACAAGGTGACGACGCCACGGTGACCAGCG‑CCTGAACACGCCCGGGATGGGTTAACGc > 1:160923/1‑239 (MQ=255) gCATCGGTAACGCCAGAACGGTTTTCTTCAACGCCCGCGCATCCTGGCGCGCCGTTTCAGTCCCCATGGTTTGTAACGCCAGGAACCGCTCCACTGTACGCTGAAAATCATCACTGAGTTGCTGCTGAAATCCCGCCAGCACGTCCGGTTTTATCCCCGGCCACTCGTCACGAGCACTAAAACAAGGTGACGACGCCACGGTGACCAGCG‑CCTGAACACGCTCGGGATGGGTTAACGCa > 1:1041333/1‑239 (MQ=255) cgggTAACGCCAGCACTTTTTTCTTCACCCCCCGCGCACCCTGGCGCGCCGTTCCATCCCCCAGGGTTTGTAACGCAGGGACCCGCCCCACTGTCCGCTGAAAATCATCCTTGGGTGGTTGCTGAATTCCCGCCAGCACGTCCGGTTTTATCCCCGGCCACTCGTCACGAGCACTAAAACAAGGTGACGACGCCACGGTGACCAGCG‑CCTGAACACGCTCGGGATGGGTTAACGCAATc < 2:829529/237‑1 (MQ=255) cGGTAACGCCAGAACGGTTTTCTTCAACGCCCGCGCATCCTGGCGCGCCGTTTCAGTCCCCATGGTTTGTAACGCCAGGAACCGCTCCACTGTACGCTGAAAATCATCACTGAGTTGCTGCTGAAATCCCGCCAGCACGTCCGGTTTTATCCCAGGCCACTCGTCACGAGCACTAAAACAAGGCGACGACGCCACGGTGACCAGCG‑CCTGAACACGCTCCGGATGGGTTAACGCaa > 1:882460/1‑236 (MQ=255) ttttCTTCAACGCCCGCGCATCCTGGCGCGCCGTTTCAGTCCCCATGGTTTGTAACGCCAGGAACCGCTCCACTGTACGCTGAAAATCATCACTGAGTTGCTGCTGAAATCCCGCCAGCACGTCCGGTTTTATCCCCGGCCACTCGTCCCGGGCCCTAAAACAAGGTGACCACGCCACGGCG‑CCACCGCCCTGAACAGCCCCGGGCAGGGTTAACCCAATCTGGCTTCCCCCCAcccc > 2:445789/1‑238 (MQ=255) ttttCTTCAACGCCCGCGCATCCTGGCGCGCCGTTTCAGTCCCCATGGTTTGTAACGCCAGGAACCGCTCCACTGTACGCTGAAAATCATCACTGAGTTGCTGCTGAAATCCCGCCAGCACGTCCGGTTTTATCCCCGGCCACTCGTCACGAGCACTAAAACAAGGTGACGACGCCACGGTGACCAGCG‑CCTGAACACGCCCGGGATGGGTTACCGCAACCTGGCCTGccccccaccc > 2:915180/1‑233 (MQ=255) cGCCCGCGCATCCTGGCGCGCCGTTTCAGTCCCCATGGTTTGTAACGCCAGGAACCGCTCCACTGTACGCTGAAAATCATCACTGAGTTGCTGCTGAAATCCCGCCAGCACGTCCGGTTTTATCCCCGGCCCCTCGTCACGAGCACTAAAACAAGGTGACGACGCCACGGTGACCAGCG‑CCTGAACACGCTCGGGATGGGTTAACGCACTCTGGCTTGCCACCAGCCACCCCAgcccc > 1:1030221/1‑234 (MQ=255) cGCCCGCGCATCCTGGCGCGCCGTTTCAGTCCCCATGGTTTGTAACGCCAGGAACCGCTCCACTGTACGCTGAAAATCATCACTGAGTTGCTGCTGAAATCCCGCCAGCACGTCCGGTTTTATCCCCGGCCACTCGTCACGAGCACTAAAACAAGGTGACGACGCCCCGGTGACCAGGG‑CCCGAACACCCCCGGGAGGGGTTAAAGCAATCTGGCTTGCCACCAACCCCCCCAAAcccc > 2:930080/1‑239 (MQ=255) ccccttCCCGGCGCGCTGTTTCATCCCCCAGTGTTTTGAACGCCAGGACCCCCCCCCCTGTACGCTGAAAATCATCACCGAGTTGCTGCTCAAATCCCGCCAGCACGTCCGGTTTTATCCCCGGCCACTCGTCACGAGCACTAAAACAAGGTGACGACGCCCCGGTGACCAGCG‑CCTGAACACGCTCGGGATGGGTTAACGCAATCTGGCTTGCCACCAGCCCGCCCAGACTCCAGCCt < 2:923320/234‑1 (MQ=255) ccTGGCGCCCCTTTTCAGTCCCCAGTGTTTGTACCGCCAGGACCCGCCCCACTGGACGCTTAAAATCATCACTGAGTTGCTGCTGAAATCCCGCCAGCACGTCCGGTTTTATCCCCGGCCACTCGTCACGAGCACTAAAACAAGGTGACGACGCCACGGTGACCAGCG‑CCTGAACACGCTCGGGATGGGTTAACGCAATCTGGCTTGCCACCAGCCCGCCCAGACTCCAGCCTAACCaa < 1:647597/239‑1 (MQ=255) ccTGGCGCCCCGTTGCAGTCCCCATGGTTTGTAACGCCAGGAACCGCCCCACTGTACGCTGAAAATCATCACTGAGTTGCTGCTGAAATCCCGCCAGCACGTCCGGTTTTATCCCCGGCCACTCGTCACGAGCACTAAAACAAGGTGACGACGCCACGGTGACCAGCG‑CCTGAACACGCTCGGGATGGGTTAACGCAATCTGGCTTGCCACCAGCCCGCCCAGACTCCAGCCTAACCaa < 1:954732/239‑1 (MQ=255) ccTGGCCCCCCGTTTCAGTCCCCATGGTTTGTAACGCCAGGAACCGCTCCCCTGTACGCTGAAAATCATCCCTGAGTTGCTGCTGAAATCCCGCCAGCACGTCCGGTTTTATCCCCGGCCACTCGTCACGAGCACTAAAACAAGGTGACGACGCCACGGTGACCAGCG‑CCTGAACACGCTCGGGATGGGTTAACGCAATCTGGCTTGCCACCAGCCCGCCCAGACTCCAGCCTAACCaa < 1:138676/239‑1 (MQ=255) ggccgcccttttCAGTCCCCATGGTTTGTACCGCCAGGAACCTCCCCACTGTTCACTAAAAACCACCCCTGGGTTGCGCGTGAAATCCCGCCAGCACGTCCGGTTTTATCCCCGGCCACTCGTCACGAGCACTAAAACAAGGTGACGACGCCACGGTGACCAGCG‑CCTGAACACGCTCGGGATGGGTTAACGCAATCTGGCTTGCCACCAGCCCGCCCAGACTCCAGCCTAACCAAATg < 2:783695/230‑1 (MQ=255) gcgcgcCGTTTCAGTCCCCATGGTTTGTAACGCCAGGAACCGCTCCACTGTACGCTGAAAATCATCACTGAGTTGCTGCTGAAATCCCGCCAGCACGTCCGGTTTTATCCCCGGCCACTCGTCACGAGCACTAAAACAAGGTGACGACGCCACGGTGACCAGCG‑CCTGAACACGCTCGGGATGGGTTAACGCAATCTGGCTTGCCACCAGCCCGCCCAGACTCCAGCCTAACCAAATgg > 1:653625/1‑239 (MQ=255) gcCGTTTCAGTCCCCATGGTTTGTAACGCCAGGAACCGCTCCACTGTACGCTGAAAATCATCACTGAGTTGCTGCTGAAATCCCGCCAGCACGTCCGGTTTTATCCCCGGCCACTCGTCACGAGCACTCAAACAAGGTGACGACGCCACGGTGACCAGCG‑CCTGACCCCGCCCGGGATGGGTTAACGCAATCTGGCTTGCCACCACCCCGCCCACCCCCCACCCTAACCAAAAGGCttt > 2:491428/1‑239 (MQ=255) cGTTTCAGTCCCCATGGTTTGTAACGCCAGGAACCGCTCCACTGTACGCTGAAAATCATCACTGAGTTGCTGCTGAAATCCCGCCAGCACGTCCGGTTTTATCCCCGGCCACTCGTCACGAGCACTAAAACAAGGTGACGACGCCACGGTGCCCAGCC‑CCTGAACACGCCCGGGATGGGTTAAAGCAATCTGGCTTGCCCACAGCCCGCCCAAACCCCCCCCAAACCAAATGCCTTTAt > 2:397396/1‑239 (MQ=255) cGTTTCAGTCCCCATGGTTTGTAACGCCAGGAACCGCTCCACTGTACGCTGAAAATCATCACTGAGTTGCTGCTGAAATCCCGCCAGCACGTCCGGTTTTATCCCCGGCCACTCGTCACGAGCACTAAAACAAGGTGACGACGCCACGGTGAACAGCG‑CCTGAACACCCTCGGGATGGGTTAACGCAATCTGGCTTGCCACCAGCCCGCCCAGCCTCCAGCCTAACCAAATGGCTTTa > 1:1027474/1‑238 (MQ=255) ttccacgccccAGGGTTTGGAACGCCGAGAACCAGCCCCCTGTCCCCTAAAAATCACCCCTGAGTTGCTGCTAAAAACCCGCCAGCACGTCCGGTTTGATCCCCGGCCACTCGTCACGAGCACTAAAACAAGGTGACGACGCCACGGTGACCAGCG‑CCTGAACACGCTCGGGATGGGTTAACGCAATCTGGCTTGCCACCAGCCCGCCCAGACTCCAGCCTAACCAAATGGCTTTATCa < 2:653625/232‑1 (MQ=255) cAGGCCCCCTGGTTTTTACGGCTAGGGACCGCTCCCCTGCCCGGTGAAAACCCTCAGCGAGGTGTTGCGCAAGCCGCGCCAGCACGTCCGGTTTTATCCCCGGCCCCTCGTCACGAGCTATAAAACAAGGTGCCGACGCCACGGTGACCAGCG‑CCTGAACACGCTCGGGATGGTTTAACGCAAGCTGGCTTGCCCCCATCCCGCCCAGACCCCAGCATAACCAACTGGCTTTATCAgat < 2:123114/239‑3 (MQ=255) gccccgaggttttGTAACGCCCGGAACCCCCCCCGCGGACGCAGAAAAGCCCCACTGGGTTGGTGGCAAAATCCCGCCAGCACGTCCGGTTTTATCCCCGGCCACTCGTCACGAGCACTAAAACAAGGTGACGACGCCACGGTGACCAGCG‑CCTGAACACGCTCGGGATGGGTTAACGCAATCTGGCTTGCCACCAGCCCGCCCAGACTCCAGCCTAACCAAATGGCTTTATCAGGTGc < 2:1033524/229‑1 (MQ=255) cccATGGTTTGTAACGCCAGGAACCGCTCCACTGTACGCTGAAAATCATCACTGAGTTGCTGCTGAAATCCCGCCAGCACGTCCGGTTTTATCCACGGCCACTCGTCACGAGCCCTATAACGAGGGACCGGCGCCACGTTGACCACGG‑CCTGAACACGCAGGGAATGGGTTAACGCAATCTGGCTTGCGAACGCCCCGCCCCAACTCCAGCCCAACCAAATGGCGTTACCAGGGGcccg > 2:235817/1‑237 (MQ=255) aTGGTTTGTAACGCCAGGAACCGCTCCACTGTACGCTGAAAATCATCACTGAGTTGCTGCTGAAATCCCGCCAGCACGTCCGGTTTTATCCCCGGCCACTCGTCACGAGCACTAAAACAAGGTGACGACGCCACGGTGACCAGCG‑CCTGAACACGCCCGGGATGGGTTAACGCAATCTGGCTTGCCACCAGCCCGCCCCGACTCCAGCCTAACCAAAAGGCTTTTCCAGGGGCCTGTTg > 2:78162/1‑239 (MQ=255) gTAACGCCAGGAACCGCTCCACTGTACGCTGAAAATCATCACTGAGTTGCTGCTGAAATCCCGCCAGCACGTCCGGTTTTATCCCCGGCCACTCGTCACGAGCACTAAAACAAGGTGACGACGCCACGGTGACCAGCG‑CCTGAACACGCTCGGGATGGGTTAACGCAATCTGGCTTGCCACCAGCCCGCCCAGCCTCCAGCCTAACCAAATGGCTTTATCAGTTGCCCGTTgcagccc > 1:4405/1‑236 (MQ=255) gTAACGCCAGGAACCGCTCCACTGTACGCTGAAAATCATCACTGAGTTGCTGCTGAAATCCCGCCAGCACGTCCGGTTTTATCCCCGGCCACTCGTCACGAGCACTAAAACAAGGTGACGACGCCACGGTGACCAGCG‑CCTGAACACGCTCGGGATGGGTTAACGCAATCTGGCTTGCCACCAGCCCGCCCAGACTCCAGCCTAACCAAATGGCTTTATCAGGTGCCTGTTGCAGCACg > 1:429805/1‑239 (MQ=255) aCGCCAGTCACCGCGCCCCTGTACCCTGAAAATCCTCCTGGAGTTGCTGTGGAAACCCCGCCAGCAACGCCGGTTTTATCCCCAGCCACGCGTCACGGGCCCTAAAACAAGGTGACGAAGCCACGGTGAACAGCG‑CCTGAACACGCCCGGGATGGGTTAACGCAATCTGGCTTGCCACCAGCCCGCCCAGACTCCAGCCTAACCAAATGGCTTTATCAGGTGCCTGTTGCAGCACGGCt < 2:701251/239‑1 (MQ=255) aCGCCAGGACCCGCTCCACTGGACGCGGAAACTCATCCCTGAGTGGATGCTGAAACCCCGCCAGCACGGCCGGTTTTATCCCCGGCCACTCGTCACGAGCACTAAAACAAGGTGACGACGCCACGGTGACCAGCG‑CCTGAACACGCTCGGGATGGGTTAACGCAATCTGGCTTGCCACCAGCCCGCCCAGACTCCAGCCTAACCAAATGGCTTTATCAGGTGCCTGTTGCAGCACGGCt < 1:78162/239‑1 (MQ=255) gacAGGTACCGCTCCACTTGCCGCTAAAACGCATCCCTGGGTTGCTGCTGAATGCCCGCCAGCACGTCCGGTTTTATCCCCGGCCACTCGTCACGAGCACTAAAACAAGGTGACGACGCCACGGTGACCAGCG‑CCTGAACACGCTCGGGATGGGTTAACGCAATCTGGCTTGCCACCAGCCCGCCCAGACTCCAGCCTAACCAAATGGCTTTATCAGGTGCCTGTTGCAGCACGGCTTc < 1:994075/237‑1 (MQ=255) ccAGGAACCGCTCCACTGTACGCTGAAAATCATCACTGAGTTGCTGCTGAAATCCCGCCAGCACGTCCGGTTTTATCCCCGGCCACTCGTCACGAGCACTAAAACAAGGTGACGACGCCACGGTGACCAGCG‑CCTGAACACGCTCGGGATGGGTTAACGCAATCTGGCTTGCCACCAGCCCGCCCAGACTCCAGCCTAACCAAATGGCTTTATCAGGTGCCTGTTGCAGCACGGCTTc > 1:701251/1‑238 (MQ=255) ccAGGAACCGCTCCACTGTACGCTGAAAATCATCACTGAGTTGCTGCTGAAATCCCGCCAGCACGTCCGGTTTTATCCCCGGCCACTCGTCACGAGCACTAAAACAAGGGGACGACGCCACGGTGACCAGCG‑CCTGAACACGCTCGGGATGGGTTAACGCAATCTGGCTTGCCACCAGCCCGCCCAGACTCCAGCCTAACCAAATGGCTTTATCAGGTGCCTGTTTCCGCACGGCTTc > 1:224877/1‑238 (MQ=255) cAGGAACCGCTCCACTGTACGCTGAAAATCATCACTGAGTTGCTGCTGAAATCCCGCCAGCACGTCCGGTTTTATCCCCGGCCACTCGTCACGAGCACTAAAACAAGGTGACGACGCCACGGTGACCAGCG‑CCTGAACACGCTCGGGATGGGTTAACGCAATCTGGCTTGCCACCAGCCCGCCCAGACTCCAGCCTACCCAAATGGCTTTATCAGGTGCCTGTTGCAGCACGGCTTCg > 1:997902/1‑238 (MQ=255) ccGGTCCACGGTCCGCTTAAAATCATCCCTGAGTGGCCGCTGAAAGCCCGCCAGCACGGCCGGTTTTATCCCCGGCCACTCGTCACGAGCACTAAAACAAGGTGACGACGCCACGGTGACCAGCG‑CCTGAACACGCTCGGGATGGGTTAACGCAATCTGGCTTGCCACCAGCCCGCCCAGACTCCAGCCTAACCAAATGGCTTTATCAGGTGCCTGTTGCAGCACGGCTTCGGCCatat < 1:637711/239‑1 (MQ=255) ccGCTCCATTGGACGCTGAAAATCATCCCTGAGTGGCTGCTGAAACCCCGCCAGCACGTCCGGTTTTATCCCCGGCCACTCGTCACGAGCACTAAAACAAGGTGACGACGCCACGGTGACCAGCG‑CCTGAACACGCTCGGGATGGGTTAACGCAATCTGGCTTGCCACCAGCCCGCCCAGACTCCAGCCTAACCAAATGGCTTTATCAGGTGCCTGTTGCAGCACGGCTTCGGCCatat < 1:732031/239‑1 (MQ=255) gCTCCACTGTACGCTGAAAATCATCACTGAGTTGCTGCTGAAATCCCGCCAGCACGTCCGGTTTTATCCCCGGCCACTCGTCACGAGCACTAAAACAAGGTGACGACGCCACGGTGACCAGCG‑CCTGAACACGCTCGGGATGGGTTACCGCAATCTGGCTTGCCACCAGCCCGCCCAGACTCCAGCCTAACCAAATGGCTTTATCAGGTGCCTGTTGCAGCACGGCTTCGGCCATATCa > 1:880114/1‑239 (MQ=255) cTCCACTGTACGCTGAAAATCATCACTGAGTTGCTGCTGAAATCCCGCCAGCACGTCCGGTTTTATCCCCGGCCACTCGTCACGAGCACTAAAACAAGGTGACGACGCCACGGTGACCAGCG‑CCTGAACACGCTCGGGATGGGTTAACGCAATCTGGCTTGCCACCAGCCCGCCCAGCCTCCAGCCTACCCAAATGGCCTTATCAGGTGCCTGTTGCAGCCCGCCCTCGGCCATATCAg > 1:485309/1‑239 (MQ=255) tCCACTGTCCGGTGAAAATCATCCCTGGGTTGCTGCTGAAACCCCGCCAGCACGTCCGGTTTTATCCCCGGCCACTCGTCACGAGCACTAAAACAAGGTGACGACGCCACGGTGACCAGCG‑CCTGAACACGCTCGGGATGGGTTAACGCAATCTGGCTTGCCACCAGCCCGCCCAGACTCCAGCCTAACCAAATGGCTTTATCAGGTGCCTGTTGCAGCACGGCTTCGGCCATATCAGc < 1:909255/239‑1 (MQ=255) cTGTACGCTGGAAATCATCACTGAGTTGCTGCTGAAATCCCGCCAGCACGTCCGGTTTTATCCCCGGCCACTCGTCACGAGCACTAAAACAAGGGGACGACGCCACGGTGACCAGCG‑CCTGAACACGCTCGGGATGGGTTAACGCAATCTGGCTTGCCACCAGCCCGCCAAGACCCCAGCATAACCAAATGGCCTTATCAGGCGCCCGTTGCAGCCCGGCTTCGGCCattccaacaacg > 2:626512/1‑229 (MQ=255) cTGTACGCTGAAAATCATCACTGAGTTGCTGCTGAAATCCCGCCAGCACGTCCGGTTTTATCCCCGGCCACTCGTCACGAGCACTAAAACAAGGTGACGACGCAACGGTGACCAGCG‑CCTGAACACGCTCGGGATGGGTTAACGCAATCTGGCTTGCAACCAGCCCGCCCAGACTCCAGCCTAACCAAATGGCTTTTTCAGGGGCCTGTTGCAGACCGGCTTCGGCCAAAACAGaaaag > 2:732031/1‑237 (MQ=255) tACGCTGAAAATCATCACTGAGTTGCTGCTGAAATCCCGCCAGCACGTCCGGTTTTATCCCCGGCCACTCGTCACGAGCACTAAAACAAGGTGACGACGCCACGGTGACCAGCG‑CCTGAACACGCTCGGGATGGGTTAACGCAATCTGGCt < 2:1017571/151‑1 (MQ=255) tACGCTGAAAATCATCACTGAGTTGCTGCTGAAATCCCGCCAGCACGTCCGGTTTTATCCCCGGCCACTCGTCACGAGCACTAAAACAAGGTGACGACGCCACGGTGACCAGCG‑CCTGAACACGCTCGGGATGGGTTAACGCAATCTGGCt > 1:1017571/1‑151 (MQ=255) tACGCTGAAAATCATCACTGAGTTGCTGCTGAAATCCCGCCAGCACGTCCGGTTTTATCCCCGGCCACTCGTCACGAGCACTAAAACAAGGTGACGACGCCACGGTGACCAGCG‑CCTGAACACGCTCGGGATGGGTTAACGCAATCTGGCTTGCCACCAGCCCGCCCAGACTCCAGCCTAACCAAATGGCTTTATCAGGGGCCTGTTGCAGCACGGCTTCGGCCATATCAGCAAGTgcc > 1:899960/1‑237 (MQ=255) tGAAAATCATCACTGAGTTGCTGCTGAAATCCCGCCAGCACGTCCGGTTTTATCCCCGGCCACTCGTCACGAGCACTAAAACAAGGTGACGACGCCACGGTGACCAGCG‑CCTGAACACGCTCGGGATGGGTTAACGCAATCTGGCTTGCCACCAGCCCGCCCAGACTCCAGCCTAACCCAATGGCTTTTTCAGGGCCCGGTTCCCGCCCGGCTTCGGCCCTATCAGCAAGTGACAccgc > 2:831496/1‑239 (MQ=255) tGAAAATCATCACTGAGTTGCTGCTGAAATCCCGCCAGCACGTCCGGTTTTATCCCCGGCCACTCGTCACGAGCACTAAAACAAGGTGACGACGCCACGGTGACCAGCG‑CCTGAACACGCTCGGGATGGGTTAACGCAATCTGGCTTGCCACCAGCCCGCCCAGACTCCAGCCTAACCAAATGGCTTTATCAGGTGCCTGTTGCAGCACGGCTTCGGc > 1:326556/1‑218 (MQ=255) aaaaTCATCACTGAGTTGCTGCTGAAATCCCGCCAGCACGTCCGGTTTTATCCCCGGCCACTCGTCACGAGCACTAAAACAAGGTGACGACGCCACGGTGACCAGCG‑CCTGAACACGCTCGGGATGGGTTAACGCAATCTGGCTTGCCACCAGCCCGCCCAGACTCCAGCCTAACCAAATGGCTTTATCAGGTGCCTGTTGCCGCACGGCTTCGGCCATATCAGCAAGGGACAGCGCa > 1:560862/1‑238 (MQ=255) aaaaTCATCACTGAGTTGCTGCTGAAATCCCGCCAGCACGTCCGGTTTTATCCCCGGCCACTCGTCACGAGCACTAAAACAAGGTGACGACGCCACGGTGACCAGCG‑CCTGAACACGCTCGGGATGGGTTAACGCAATCTGGCTTGCCACCAGCCCGCCCAGACTCCAGCCTAACCAAATGGCTTTACCAGGCGCCACTTGCAACACGGCCTCGCCCAAAACAACAAGGGACAgcgcca > 2:490961/1‑237 (MQ=255) atcatcACTGAGTTGCTGCTGAAATCCCGCCAGCACGTCCGGTTTTATCCCCGGCCACTCGTCACGAGCACTAAAACAAGGCGACGACGCCACGGTGACCAGCG‑CCTGAACACGCTCGGGATGGGTTAACGCAATCTGGCTTGCCACCAGCCCGCCCAGCCTCCAGCCTAACCAAATGGCTTTATCAGGGGCCTGTTGCCGACCGGCTTCGGCCAAATCAGCAAGTGACAGGCCACCaa > 2:692264/1‑239 (MQ=255) catcaCTGAGTTGCTGCTGAAATCCCGCCAGCACGTCCGGTTTTATCCCCGGCCACTCGTCACGAGCACTAAAACAAGGTGACGACGCCACGGTGACCAGCG‑CCTGAACACGCTCGGGATGGGTTAACGCAATCTGGCTTGCCACCAGCCCGCCCAGACCCCAGCCTAACCAAATGGCTTTATCAGGTGCCTGTTGCAGCACGGCTTCGGCCATATCACCAAGTGAAAGCCCACCcaa > 1:437588/1‑238 (MQ=255) | ACCAGACCGTCGAGATAGCCATACAATCGCAAAAACGGCATGGACACGTTTTGCAGCGGCTGACGGAGATCGACCGTTTTCAGGATTTCCAGCCCGCCATTAAGCACGTCAACCTCCGGCATCGGTAACGCCAGAACGGTTTTCTTCAACGCCCGCGCATCCTGGCGCGCCGTTTCAGTCCCCATGGTTTGTAACGCCAGGAACCGCTCCACTGTACGCTGAAAATCATCACTGAGTTGCTGCTGAAATCCCGCCAGCACGTCCGGTTTTATCCCCGGCCACTCGTCACGAGCACTAAAACAAGGTGACGACGCCACGGTGACCAGCG‑CCTGAACACGCTCGGGATGGGTTAACGCAATCTGGCTTGCCACCAGCCCGCCCAGACTCCAGCCTAACCAAATGGCTTTATCAGGTGCCTGTTGCAGCACGGCTTCGGCCATATCAGCAAGTGACAGCGCACCAAA > NC_000913/3544216‑3544679 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |