Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 647,210 A→C 23.6% G100G (GGT→GGG citG ← 2‑(5''‑triphosphoribosyl)‑3'‑dephosphocoenzyme‑A synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913647,2100AC23.6% 83.3 / 5.0 52G100G (GGT→GGGcitG2‑(5''‑triphosphoribosyl)‑3'‑dephosphocoenzyme‑A synthase
Reads supporting (aligned to +/- strand):  ref base A (29/8);  new base C (2/12);  total (31/21)
Fisher's exact test for biased strand distribution p-value = 4.72e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCGCCGGTAAGGCCAAGCTGTTGGTACAACCGTTGACCTGCCGTCAGTTGTGAATTATTGGTACGCAGTTCGCGATCGGTCAGGCCACGGCAGAAACTTGCCGCCGTAGAACAAACGGTTGTTGGCGTTACCGGTTGGTTGAGTTGAAGCAAACGGCCAATTGCCGCACATAGCAGCCCTAAAGAAAAAATGCTGCCTTTATGCGTGTTTACGCCCGCAGTGGCGCGGAACATATCACCTTCGCAAGCCATACCAATTGGGCGTAATCCGTGGAGTACCGCTTCTGGTGCCATTTCCGCACTACAGGCACCAAATTCAATGAAACGGGGTAGCCAGCCCTGAATCGCCAGCGCGCTGCGGTGGAAATCTTCCAGCGCCATATCTTTGTGCGCACCGCAGTTAATGCGATCCACGAGGCCTGGTTTCGGTGACAGATTGACTTCAGTCAGCATGGCGCGCCAGCCCAGCAGGG  >  NC_000913/646974‑647445
                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                           
ggcccGGTAGGGCAAATGTTTTGGTACAACTGTGTCCCTGCCGTAGTTTGGAAATTATTGGTCGGCAGTCGGCGAGGGGTCAGGCCAGGGAAGAAACTTGCCGCCGTAGAACAAACGGTTGTTGGCGTTACCGGTTGGTTGAGTTGAAGCAAACGGCCAATTGCCGCACATAGCAGCCCTAAAGAAAAAATGCTGCCTTTATGCGTGTTTACGCCCGCAGTGGCGCGGAACATATCAc                                                                                                                                                                                                                                            <  2:1073115/235‑1 (MQ=255)
                gCTGTTGGTACAACCGTTGACCTGCCGTCAGTTGTGAATTATTGGTACGCAGTTCGCGATCGGTCAGGCCACGGCAGAAACTTGCCGCCGTAGAACAAACGGTTGTTGGCGTTACCGGTTGGTTGAGTTGAAGCAAACGGCCAATTGCCGCACATAGCAGCCCTAAAGAAAAAATGCTGCCTTTATGCGTGTTTACGCCCGCAGTGGCGCGGAACATATCACCTTCGCAAGCCCTACCa                                                                                                                                                                                                                           >  1:716778/1‑239 (MQ=255)
                gCTGTTGGTACAACCGTTGACCTGCCGTCAGTTGTGAATTATTGGTACGCAGTTCGCGATCGGTCAGGCCACGGCAGAAACTTGCCGCCGTAGAACAAACGGTTGTTGGCGTTACCGGTTGGTTGAGTTGAAGCAAACGGCCAATTGCCGCACATAGCAGCCCTAAAGAAAAAATGCTGCCTTTATGCGTGTTTACGCCCGCAGTGGCGCGGAACATATCACCTTCGCAAGCCATACCa                                                                                                                                                                                                                           >  1:176203/1‑239 (MQ=255)
                     tGGTACAACCGTTGACCTGCCGTCAGTTGTGAATTATTGGTACGCAGTTCGCGATCGGTCAGGCCACGGCAGAAACTTGCCGCCGTAGAACAAACGGTTGTTGGCGTTACCGGTTGGTTGAGTTGAAGCAAACGGCCAATTGCCGCACATAGCAGCCCTAAAGAAAAAATGCTGCCTTTATGCGTGTTTACGCCCGCCGTGGCGCGGAACAAATCACCTTCGCAAGCCATACCAATTgg                                                                                                                                                                                                                      >  1:1007646/1‑239 (MQ=255)
                     tGGTACAACCGTTGACCTGCCGTCAGTTGTGAATTATTGGTACGCAGTTCGCGATCGGTCAGGCCACGGCAGAAACTTGCCGCCGTAGAACAAACGGTTGTTGGCGTTACCGGTTGGTTGAGTTGAAGCAAACGGCCAATTGCCGCACATAGCAGCCCTAAAGAAAAAATGCTGCCTTTATGCGTGTTTACGCCCGCAGTGGCGCGGAACATATCACCTTCGCAAGCCATACCAATTgg                                                                                                                                                                                                                      >  1:697561/1‑239 (MQ=255)
                     tGGTACAACCGTTGACCTGCCGTCAGTTGTGAATTATTGGTACGCAGTTCGCGATCGGTCAGGCCACGGCAGAAACTTGCCGCCGTAGAACAAACGGTTGTTGGCGTTACCGGTTGGTTGAGTTGAAGCAAACGGCCAATTGCCGCACATAGCAGCCCTAAAGAAAAAATGCTGCCTTTATGCGTGTTTACGCCCGCAGTGGCGCGGAACATATAACCTTCGCAAGCCATACCAATTgg                                                                                                                                                                                                                      >  1:591756/1‑239 (MQ=255)
                      ggTAAAACCTTTGACCTGCCGTCGGTGGTGAATTTTTGGTCCGCAGTTCGCGATCGGTCAGGCCACGGCAAAAATTTGCCGCCGTAGAACAAACGGTTGTTGGCGTTACCGGTTGGTTGAGTTGAAGCAACCGGCCAATTGCCGCACATAGCAGCCCTAAAGAAAAAATGCTGCCTTTATGCGTGTTTACGCCCGCAGTGGCGCGGAACATATCACCTTCGCAAGCCATACCAAt                                                                                                                                                                                                                         <  1:279826/235‑1 (MQ=255)
                            cctgtgtcctggCCGTCGTTTGTAAATTATGGTGACGCAGTTGGGGTTCGGTCGGGCCACGGCAGAAACTTGCCGCCGTAGAACAAACGGTTGTTGGCGTTACCGGTTGGTTGAGTTGAAGCAAACGGCCAATTGCCGCACATAGCAGCCCTAAAGAAAAAATGCTGCCTTTATGCGTGTTTACGCCCGCAGTGGCGCGGAACATATCCCCTTCGCAAGCCATACCAATTGGGCGTAAt                                                                                                                                                                                                               <  1:1082074/228‑1 (MQ=255)
                              cGTTGACCTGCCGTCAGTTGTGAATTATTGGTACGCAGTTCGCGATCGGTCAGGCCACGGCAGAAACTTGCCGCCGTAGAACAAACGGTTGTTGGCGTTACCGGTTGGTTGAGTTGAAGCAAACGGCCAATTGCCGCACATCGCAGCCCTAAAGAAAAACTCCTGCCTTTATGCGTGTTTACGCCCGCAGGGGCCGGGAACATATCACCTTTCCAAGCCCTACCCATTTGGCGAAAccc                                                                                                                                                                                                             >  2:52817/1‑239 (MQ=255)
                                ttGACCTGCCGTCAGTTGTGAATTATTGGTACGCAGTTCGCGATCGGTCAGGCCACGGCAGAAACTTGCCGCCGTAGAACAAACGGTTGTTGGCGTTACCGGTTGGTTGAGTTGAAGCAAACGGCCAATTGCCGCACATAGCAGCCCTAAAGAAAAAATGCTGCCTTTATGCGTGTTTACGCCCGCAGTGGCGCGGAACATATCACCTTCGCAAGCCATACCAATTGGGCGTAATCCGt                                                                                                                                                                                                           >  1:401506/1‑239 (MQ=255)
                                ttGACCTGCCGTCAGTTGTGAATTATTGGTACGCAGTTCGCGATCGGTCAGGCCACGGCAGAAACTTGCCGCCGTAGAACAAACGGTTGTTGGCGTTACCGGTTGGTTGAGTTGAAGCAAACGGCCAATTGCCGCACATAGCAGCCCTAAAGAAAAAATGCTGCCTTTATGCGTGTTTACGCCCGCACTGGGGCGGAACAAATCACCTTCGCAAGCCATCCCAATTGGGCGGAACCCg                                                                                                                                                                                                            >  2:279826/1‑238 (MQ=255)
                                ttGACCTGCCGTCAGTTGTGAATTATTGGTACGCAGTTCGCGATCGGTCAGGCCACGGCAGAAACTTGCCGCCGTAGAACAAACGGTTGTTGGCGTTACCGGTTGGTTGAGTTGAAGCAAACGGCCAATTGCCGCACATAGACGCCCTAAAGAAAAAAAGCTGCCTTTATGAGTTTTTAAGCCCCCACTGGCCGGGAAACAAACCCCTTCGCAAGCCCTACCAATTggcgggaagccgg                                                                                                                                                                                                           >  2:717854/1‑228 (MQ=255)
                                 tGACCTGCCGTCAGTTGTGAATTATTGGTACGCAGTTCGCGATCGGTCAGGCCACGGCAGAAACTTGCCGCCGTAGAACAAACGGTTGTTGGCGTTACCGGTTGGTTGAGTTGAAGCAAACGGCCAATTGCCGCACATAGCAGCCCTAAAGAAAAAATGCTGCCTTTATGCGTGTTTACGCCCGCAGTGGCGCGGAACATATCACCTTCGCAAGCCATACCAATTGGGCGTAATCCGTg                                                                                                                                                                                                          >  1:14685/1‑239 (MQ=255)
                                  gACCTGCCGTCAGTTGTGAATTATTGGTACGCAGTTCGCGATCGGTCAGGCCACGGCAGAAACTGGCCGCCGTAGAACAAACGGTTGTTGGCGTTACCGGTTGGTTGAGTTGAAGCAAACGGCCAATTGCCGCACATAGCAGCCCTAAAGAAAAAATGCTGCCTTTATGCGTGTTTACGCCCGCAGTGGCGCGGAACATATCACCTTCGCAAGCCATACCAATTGGGCGTAATCCGTgg                                                                                                                                                                                                         <  1:127690/239‑1 (MQ=255)
                                       gCCGTCAGTTGTGAATTATTGGTACGCAGTTCGCGATCGGTCAGGCCACGGCAGAAACTTGCCGCCGTAGAACAAACGGTTGTTGGCGTTACCGGTTGGTTGAGTTGCAGCAAACGGCCAATTGCCGCACATAGCAGCCCTAAAGAAAAAATGCTGCCTTTATGCCGGTTTACGCCCGCAGTGGCGCGGAACAATTCCCCTTCCCAAGCCATACCAATTGGGCGTAATcctgggggac                                                                                                                                                                                                     >  2:861205/1‑230 (MQ=255)
                                       gCCGTCAGTTGTGAATTATTGGTACGCAGTTCGCGATCGGTCAGGCCACGGCAGAAACTTGCCGCCGTAGAACAAACGGTTGTTGGCGTTACCGGTTGGTTGAGTTGAAGCAAACGGCCAATTGCCGCACATAGCAGCCCTAAAGAAAAAATGCTGCCTTTATTCGTGTTTACGCCCGCAGTGGCACGGAAAATATCACCTTCGCAAGCCCTACCAATTGGGCTGAAACCGTGGAgaa                                                                                                                                                                                                     >  1:441991/1‑236 (MQ=255)
                                                      ttttgtgtccgcgGTTGGCGATGGGGAGGGCCAGGGCAAAACTTGGCCGCAGTAGAACAAACGGTTTTTGGCGTTCCGGGTTGTTTGAGTTGAAGCAAACGGCCAATTGCCGCACATAGCAGCCCTAAAGAAAAAATGCTGCCTTTATGCGTGTTTACGCCCGCAGTGGCGCGGAACATATCACCTTCGCAAGCCATACCAATTGGGCGTAATCCGTGGAGTACCGCTTCTGGTGCCAt                                                                                                                                                                                     <  1:318962/230‑1 (MQ=255)
                                                         ttGGTACGCAGTTCGCGATCGGTCAGGCCACGGCAGAAACTTGCCGCCGTAGAACAAACGGTTGTTGGCGTTACCGGTTGGTTGAGTTGAAGCAAACGGCCAATTGCCGCACATAGCAGCCCTAAAGAAAAAATGCTGCCTTTATGCGTGTTTACGCCCGCAGTGGCGCGGAACATATCACCTTCGCAAGCCATACCAATTGGGCGTAATCCGTGGAGTACCGCTTCTGGTGCCAttt                                                                                                                                                                                   >  1:98749/1‑238 (MQ=255)
                                                                      cgcgATCGGTCAGGCCACGGCAGAAACTTGCCGCCGTAGAACAAACGGTTGTTGGCGTTACCGGTTGGTTGAGTTGAAGCAAACGGCCAATTGCCGCACATAGCAGCCCTAAAGAAAAAATGCTGCCTTTATGCGGTTTTCCGCCCGCAGGGGCCCGGAACATATCACCTTCGCAAGCCATCCCCATTGGGCGTAATCCGTGGGGTCCCCCTCCTGGTGCCATTTCCCCCCTAAAGGc                                                                                                                                                                      >  2:86620/1‑238 (MQ=255)
                                                                              gTCAGGCCACGGCAGAAACTTGCCGCCGTAGAACAAACGGTTGTTGGCGTTACCGGTTGGTTGAGTTGAAGCAAACGGCCAATTGCCGCACATAGCAGCCCTAAAGAAAAAATGCTGCCTTTATGCGTGTTTACGCCCGCAGTGGCGCGGAACATATCACCTTCGCAAGCCATACCAATTGGGCGTAATCCGTGGAGTACCGCTTCTGGTGCCATTTCCGCACTACAGGCACCAAATTc                                                                                                                                                             >  1:516768/1‑239 (MQ=255)
                                                                              gTCAGGCCACGGCAGAAACTTGCCGCCGTAGAACAAACGGTTGTTGGCGTTACCGGTTGGTTGAGTTGAAGCAAACGGCCAATTGCCGCACATAGCAGCCCTAAAGAAAAAATGCTGCCTTTATGCGTGTTTACGCCCGCAGTGGCCCGGAACATATCACCTTCGCAAGCCATACCAATTGGGCGTACACCGTGGAGTCCCGCTTCTGGTGCCATTTCCGCACCTCAGTCACCAAATTc                                                                                                                                                             >  1:825460/1‑239 (MQ=255)
                                                                                     cccGGCAAAAACTTGCCGCCGTAGAACAAACGGTTGTTGGCTTTACCGGTTGGTTGAGTTGAAGAAAACGGCCAATTGCCGCACATAGCAGCCCTAAAGAAAAAATGCTGCCTTTATGCGTGTTTACGCCCGCAGTGGCGCGGAACATATCCCCTTCGCAAGCCATACCAATTGGGCGTAATCCGTGGAGTACCGCTTCTGGTGCCATTTCCGCACTACAGGCACCAAATTCAATGaa                                                                                                                                                       <  1:761182/236‑1 (MQ=255)
                                                                                        ggCAGAAACTTGCCGCCGTAGAACAAACGGTTGTTGGCGTTACCGGTTGGTTGAGTTGAAGCAAACGGCCAATTGCCGCACATAGCAGCCCTAAAGAAAAAATGCTGCCTTTATGCGTGTTTACGCCCGCAGTGGCGCGGAACATATCACCTTCGCAAGCCATACCAATTGGGCGTAATCCGTGGAGTACCGCTTCTGGTGCCATTTCCGCACTACAGGCACCAAATTCAATGAAACgg                                                                                                                                                   >  1:790885/1‑239 (MQ=255)
                                                                                          cAGAAATTTGCCGCCGTAGAACAAACGGTTGTTGGCTTTACCGGTTGGTTGAGTTGAAGCAAACGGCCAATTGCCGCAAATAGCAGCCCTAAAGAAAAAATGCTGCCTTTATGCGTGTTTACGCCCGCAGTGGCGCGGAACATATCACCTTCGCAAGCCATACCAATTGGGCGTAATCCGTGGAGTACCGCTTCTGGTGCCATTTCCGCACTACAGGCACCAAATTCAATGAAACggg                                                                                                                                                  <  1:730872/238‑1 (MQ=255)
                                                                                           aaaaattttcccggcgGAGAACAACTGTTTGTGGGGGTTACTGGTTGGTTGAGTGGAAGCAAACGGCCACTTGCCGCACATAGCACCCCTAAAGAAAAAATGCTGCCTTTAGGGGTGGTTCCGCCCGCAGTGGCGGGGAACATATCCCCTTCGCAAGCCATACCAATTGGGCGTAATCCGTGGAGTACCGCTTCTGGTGCCATTTCCGCACTACAGGCACCAAATTCAATGAAACggg                                                                                                                                                  <  2:1052370/228‑1 (MQ=255)
                                                                                             aaaCTTGCCGCCGTAGAACAAACGGTTGTTGGCGTTACCGGTTGGTTGAGTTGAAGCAAACGGCCAATTGCCGCACATAGCAGCCCTAAAGAAAAAATGCTGCCTTTATGCGTGTTTACGCCCGCAGTGGCGCGGAACATATCACCTTCGCAAGCCATACCAATTGGGCGTAATCCGTgg                                                                                                                                                                                                         <  1:626733/180‑1 (MQ=255)
                                                                                             aaaCTTGCCGCCGTAGAACAAACGGTTGTTGGCGTTACCGGTTGGTTGAGTTGAAGCAAACGGCCAATTGCCGCACATAGCAGCCCTAAAGAAAAAATGCTGCCTTTATGCGTGTTTACGCCCGCAGTGGCGCGGAACATATCACCTTCGCAAGCCATACCAATTGGGCGTAATCCGTgg                                                                                                                                                                                                         >  2:626733/1‑180 (MQ=255)
                                                                                             aaaCTTGCCGCCGTAGAACAAACGGTTGTTGGCGTTACCGGTTGGTTGAGTTGAAGCAAACGGCCAATTGCCGCACATAGCAGCCCTAAAGAAAAAATGCTGCCTTTATGCGTGTTTACGCCCGCAGTGGCGCGGAACATATCACCTTCGCAAGCCATACCAATTGGGCGTAATCCGTGGAGTACCGCTTCTGGTGCCATTTCCGCACTACAGGCACCAAATTCAATGAAACGGGGtg                                                                                                                                               >  1:218488/1‑237 (MQ=255)
                                                                                              aaCGTGCCGCCGTAAAAAAAACGGTTGTGGGCGTTACCGGTTGGTTGAGTTGAAGCAAACGGCCAATTGCCGCAAATAGCAGCCCTAAAGAAAAAATGCTGCCTTTATGGGTGTTTACGCCCGCAGTGGCGCGGAACATATCACCTTCGCAAGCCATACCAATTGGGCGTAATCCGTGGAGTACCGCTTCTGGTGCCATTTCCGCACTACAGGCACCAAATTCAATGAAACGGGGTagc                                                                                                                                             <  1:197144/239‑1 (MQ=255)
                                                                                                     ccgccgTAGAACAAACGGTTGTTGGCGTTACCGGTTGGTTGAGTTGAAGCAAACGGCCAATTGCCGCACATAGCAGCCCTAAAGAAAAAATGCTGCCTTTATGCGTGTTTACGCCCGCAGTGGCGCGGAACATATCACCTTCGCAAGCCATACCAATTGGGCGAAATCCGTGGAGTACCGCTTCTGGGGCCCTTTCCCACCTACAGGCACCAAATTCAATAAAACGGGGTAACCAGccc                                                                                                                                       >  2:925676/1‑239 (MQ=255)
                                                                                                                                       ttggttgAGTTGAAGCAACCGGCCAATTGCCGCACATAGCAGCCCTAAAGAAAAAATGCTGCCTTTATGCGTTTTTACGCCCGCAGTGGCGCGGAACATATCACCTTCGCAAGCCATACCAATTGGGCGTAATCCGTGGAGTACCGCTTCTGGTGCCATTTCCGCACTACAGGCACCAAATTCAATGAAACGGGGTAGCCAGCCCTGAATCGCCAGCGCGCTGCGGTGGAAATCTTcc                                                                                                      <  1:306667/238‑1 (MQ=255)
                                                                                                                                        tggtttggttGAACCAACCGGCCATTTGCCGTACAAAGCAGCCCTAAAGAAAAACTGCTGCCTTTATGCTTGTTTCCGCCCGCATGGGCGCGGAACATATCCCCTTCGCAAGCCATACCAATTGGGCGTAATCCGTGGAGTACCGCTTCTGGTGCCATTTCCGCACTACAGGCACCAAATTCAATGAAACGGGGTAGCCAGCCCTGAATCGCCAGCGCGCTGCGGTGGAAATCTTCCa                                                                                                     <  1:757919/238‑1 (MQ=255)
                                                                                                                                            tgattgaaaGCAACGGGACATTGGCCGCACATGACAGCCATAAAAAAAAAATGCTGCTTTTTGGCGTTTTTCCGCCCGCGGTGGCGGGGATAATATCCCCTGCGAAAGCCATACCAATTGGGCGTAATCCGTGGAGTACCGCTTCTGGTGCCATTTCCGCACTACAGGCACCAAATTCAATGAAACGGGGTAGCCAGCCCTGAATCGCCAGCGCGCTGCGGTGGAAATCTTCCAGCGcc                                                                                                <  2:1058094/232‑1 (MQ=255)
                                                                                                                                            tgagttgaAGAAAGCGGCAAATGGCCGCACAGAGCAGCCCAAAAGAAAAACGGCTGCCTTTATGCGTGTTTACGCCCGCAGTGGCGCGGAACATATCCCCTTCGCAAGCCATACCAATTGGGCGTAATCCGTGGAGTACCGCTTCTGGTGCCATTTCCGCACTACAGGCACCAAATTCAATGAAACGGGGTAGCCAGCCCTGAATCGCCAGCGCGCTGCGGTGGAAATCTTCCAGCGcc                                                                                                <  1:465529/239‑1 (MQ=255)
                                                                                                                                              agttgaAGCAAACGGCCAATTGCCGCACATAGCAGCCCTAAAGAAAAAATGCTGCCTTTATGCGTGTTTACGCCCGCAGTGGCGCGGAACATATCACCTTCGCAAGCCATACCAATTGGGCGTAATCCGTGGAGTACCGCTTCTGGTGCCATTTCCGCACTAAAGGCACCAAATTCAATGAAACGGGGTAGCCAGCCCTGAATCGCCAGCGCGCTGCGGTGGAAATCTTCCAGCGCCat                                                                                              >  1:1033245/1‑239 (MQ=255)
                                                                                                                                                            cccagtcgccgccAATAGCCGCGCTAAAGAAAAATGTCGTCTTTTCGGGGTTTTTACCCCGGCAGTGGCGCGGACGTGATCGCCTCCGCAAGCCATACCAATTGGGCGTAATCCGTGGAGTACCGCTTCTGGTGCCATTTCCGCACTACAGGCACCAAATTCAATGAAACGGGGTAGCCAGCCCTGAATCGCCAGCGCGCTGCGGTGGAAATCTTCCAGCGCCATATCTTTGTGCGCAc                                                                                <  2:497720/232‑1 (MQ=255)
                                                                                                                                                              caatgcccGCACATAGCAGCCCTAAAGAAAAAATGCTGCCTTTATGCGTGTTTACGCCCGCAGGGGCGCGGAAAATATCCCCTTCGCAAGCCATACCAATTGGGCGTAATCCGTGGAGTACCGCTTCTGGTGCCATTTCCGCACTACAGGCACCAAATTCAATGAAACGGGGTAGCCAGCCCTGAATCGCCAGCGCGCTGCGGTGGAAATCTTCCAGCGCCATATCTTTGTGCGCAcc                                                                               <  1:659159/232‑1 (MQ=255)
                                                                                                                                                                     cGCACATAGCAGCCATAAAGAAAAAATGCTGCCTTTAGGCGTGTTTCCGCCCGCAGTGGCGCGGAACATATCCCCTTCGCAAGCCATACCAATTGGGCGTAATCCGTGGAGTACCGCTTCTGGTGCCATTTCCGCACTACAGGCACCAAATTCAATGAAACGGGGTAGCCAGCCCTGAATCGCCAGCGCGCTGCGGTGGAAATCTTCCAGCGCCATATCTTTGTGCGCACCGCAGTTaa                                                                       <  1:861205/239‑1 (MQ=255)
                                                                                                                                                                       cacaTAGCAGCCCTAAAGAAAAAATGCTGCCTTTATGCGTGTTTACGCCCGCAGTGGCGCGGAACATATCACCTTCGCAAGCCATACCAATTGGGCGTAATCCGTGGAGTACCGCTTCTGGTGCCATTTCCGCACTACAGGCCCCAAATTCAATGAAACGGGGTAGCCAGCCCTGAATAGCCCACCCCGTCGGCGGGAAAACTTCCACCGCCATACCTTGTTGCGCACCCCAGTGaaag                                                                     >  2:193197/1‑237 (MQ=255)
                                                                                                                                                                              cagcCCTAAAGAAAAAATGCTGCCTTTATGCGTGTTTACGCCCGCAGTGGCGCGGAACATATCACCTTCGCAAGCCATACCAATTGGGCGTAATCCGTGGAGTACCGCTTCTGGTGCCATTTCCGCACTACAGGCACCAAATTCAATGAAACGGGGTAGCCAGCCCTGAATCGCCAACGCGCCGCGGGGGAAATCTTCCAGCGCCATATCTTTGTGCCCCCCGCAGTTAAtcggaccc                                                               >  2:83645/1‑231 (MQ=255)
                                                                                                                                                                                gcCCTAAAGAAAAAATGTTGCCTTGAGGCGTGTTTACCCCCGAGGGGGCGGGGAACTTTCCCCCTTGGCAAGCCATACCAATTGGGCGTAATCCGTGGAGTACCGCTTCTGGTGCCATTTCCGCACTACAGGCACCAAATTCAATGAAACGGGGTAGCCAGCCCTGAATCGCCAGCGCGCTGCGGTGGAAATCTTCCAGCGCCATATCTTTGTGCGCACCGCAGTTAATGCGATCCACg                                                            <  1:889892/239‑1 (MQ=255)
                                                                                                                                                                                  ccTAAAGAAAAAAGGCTGCCTTTATGCGTGTTTACGCCCGCAGTGGCGCGGAACATATCCCCTTCGCAAGCCATACCAATTGGGCGTAATCCGTGGAGTACCGCTTCTGGTGCCATTTCCGCACTACAGGCACCAAATTCAGTGAAACGGGGTAGCCAGCCCTGAATCGCCAGCGCGCTGCGGTGGAAATCTTCCAGCGCCATATCTTTGTGCGCACCGCAGTTAATGCGATCCAc                                                             <  1:845240/236‑1 (MQ=255)
                                                                                                                                                                                  caaaaaGAAAAAATGCTGCCTTTAGGCGGTTTTACGCCCGCAGTGGCGCGGAACATATCCCCTTCGCAAGCCATACCAATTGGGCGTAATCCGTGGAGTACCGCTTCTGGTGCCATTTCCGCACTACAGGCACCAAATTCAATGAAACGGGGTAGCCAGCCCTGAATCGCCAGCGCGCTGCGGTGGAAATCTTCCAGCGCCATATCTTTGTGCGCACCGCAGTTAATGCGATCCACg                                                            <  1:86620/234‑1 (MQ=255)
                                                                                                                                                                                      aaGAAAAAATGCTGCCTTTATGCGTGTTTACGCCCGCAGTGGCGCGGAACATATCACCTTCGCAAGCCATACCAATTGGGCGTAATCCGTGGAGTACCGCTTCTGGTGCCATTTCCGCACTACAGGCACCAAATTCAATGAAACGGGGTAGCCAGCCCTGAATCGCCAGCGCGCTGCGGTGGAAATCTTCCAGCGCCATATCTTTGTGCGCACCGCAGTTAATCCGATCCACGAgg                                                         >  1:904358/1‑236 (MQ=255)
                                                                                                                                                                                                     tttttgtcGTTTTTCCCCCCGGCGTGGCGCGGAACATTTCCCTTTCGCAAGCCATACCAATTGGGCGTAATCCGTGGAGTACCGCTTCTGGTGCCATTTCCGCACTACAGGCACCAAATTCAATGAAACGGGGTAGCCAGCCCTGAATCGCCAGCGCGCTGCGGTGGAAATCTTCCAGCGCCATATCTTTGTGCGCACCGCAGTTAATGCGATCCACGAGGCCTGGTTTCGGTGACAGa                                       <  2:1033245/232‑1 (MQ=255)
                                                                                                                                                                                                              tgtTTACGCCCGCAGTGGCGCGGAACATATCACCTTCGCAAGCCATACCAATTGGGCGTAATCCGTGGAGTACCGCTTCTGGTGCCATTTCCGCACTACAGGCACCAAATTCAATGAAACGGGGTAGCCAGCCCTGAATCGCCAGCGCGCTGCGGTGGAAATCTTCCAGCGCCCTATCTTTGTGCGCACCGCAGTTAATGCGATCCACGAGGCCTGGATTCGGTGCAAGATTGGCTtca                              >  1:860598/1‑239 (MQ=255)
                                                                                                                                                                                                              tgtTTACGCCCGCAGTGGCGCGGAACATATCACCTTCGCAAGCCATACCAATTGGGCGTAATCCGTGGAGTACCGCTTCTGGTGCCATTTCCGCACTACAGGCACCAAATTCAATGAAACGGGGTAGCCAGCCCTGAATCGCCAGCGCGCTGCGGTGGAAATCTTCCAGCGCCATATCTTTGTGCGCACCGCAGTTAATGCGATCCACGAGGCCTGGTTTCGGTGACAGATTGACTtca                              >  1:908414/1‑239 (MQ=255)
                                                                                                                                                                                                              tgtTTACGCCCGCAGTGGCGCGGAACATATCACCTTCGCAAGCCATACCAATTGGGCGTAATCCGTGGAGTACCGCTTCTGGTGCCATTTCCGCACTACAGGCACCAAATTCAATGAAACGGGGTAGCCAGCCCTGAATCGCCAGCGCGCTGCGGTGGAAATCTTCCAGCGCCATATCTTTGTGCGCACCGCAGTTAATGCGATCCACGAGGCCTGGTTTCGGTGACAGATTGACTtc                               >  1:264924/1‑238 (MQ=255)
                                                                                                                                                                                                              tgtTTACGCCCGCAGTGGCGCGGAACATATCACCTTCGCAAGCCATACCAATTGGGCGTAATCCGTGGAGTACCGCTTCTGGTGCCATTTCCGCACTACAGGCACCAAATTCAATGAAACGGGGTAGCCAGCCCTGAATCGCCAGCGCGCTGCGGTGGAAACCGTCCACAGCCATATCTTTGTGCCCACCCCAGTTAAGTCGCTCCCCGAGCCCTGGTTCCGGTGACAGAtgtacgtca                              >  1:649825/1‑231 (MQ=255)
                                                                                                                                                                                                                tttACGCCCGCAGTGGCGCGGAACATATCACCTTCGCAAGCCATACCAATTGGGCGTAATCCGTGGAGTACCGCTTCTGGTGCCATTTCCGCACTACAGGCACCAAATTCAATGAAACGGGGTAGCCAGCCCTGAATCGCCAGCGCGCTGCGGTGGAAATCTTCCAGCGCCAGATCTTTGTGCGACCCCCAGTTAATGCGATCCACGGGGCCAGGTTTCGGTGAAAGAATGACCtcgga                            >  2:160168/1‑236 (MQ=255)
                                                                                                                                                                                                                               gcgcGGAACATATCACCTTCGCAAGCCATACCAATTGGGCGTAATCCGTGGAGTACCGCTTCTGGTGCCATTTCCGCACTACAGGCACCAAATTCAATGAAACGGGGTAGCCAGCCCTGAATCGCCAGCGCGCTGCGGTGGAAATCTTCCAGCGCCATATCTTTGTGCGCACCGCAGTTAATGCGATCCACGCGGCCTGGt                                                   >  1:129623/1‑201 (MQ=255)
                                                                                                                                                                                                                                          atCACCTTCGCAAGCCATACCAATTGGGCGTAATCCGTGGAGTACCGCTTCTGGTGCCATTTCCGCACTACAGGCACCAAATTCAATGAAACGGGGTAGCCAGCCCTGAATCGCCAGCGCGCTGCGGTGGAAATCTTCCAGCGCCATATCTTTGTGCGCACCGCAGTTAATGCGATCCACAAGGCCTGGTTTCGGTGACAGATTGCCTTCAGTCAGCTTTGCGCGCCAGCCCAGCCggg  >  1:862440/1‑239 (MQ=255)
                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                           
GCGCCGGTAAGGCCAAGCTGTTGGTACAACCGTTGACCTGCCGTCAGTTGTGAATTATTGGTACGCAGTTCGCGATCGGTCAGGCCACGGCAGAAACTTGCCGCCGTAGAACAAACGGTTGTTGGCGTTACCGGTTGGTTGAGTTGAAGCAAACGGCCAATTGCCGCACATAGCAGCCCTAAAGAAAAAATGCTGCCTTTATGCGTGTTTACGCCCGCAGTGGCGCGGAACATATCACCTTCGCAAGCCATACCAATTGGGCGTAATCCGTGGAGTACCGCTTCTGGTGCCATTTCCGCACTACAGGCACCAAATTCAATGAAACGGGGTAGCCAGCCCTGAATCGCCAGCGCGCTGCGGTGGAAATCTTCCAGCGCCATATCTTTGTGCGCACCGCAGTTAATGCGATCCACGAGGCCTGGTTTCGGTGACAGATTGACTTCAGTCAGCATGGCGCGCCAGCCCAGCAGGG  >  NC_000913/646974‑647445

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: