Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 665,104 A→C 16.9% S29R (AGT→AGG rlpA ← septal ring protein, suppressor of prc, minor lipoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913665,1040AC16.9% 158.5 / 4.9 76S29R (AGT→AGGrlpAseptal ring protein, suppressor of prc, minor lipoprotein
Reads supporting (aligned to +/- strand):  ref base A (33/27);  new base C (4/11);  total (37/39)
Fisher's exact test for biased strand distribution p-value = 8.16e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01

AGCTGTGTCGGATCGAATGCTTCGCCAGAGGCCGTCAGGTTACTGCCGGGTTCGGCATCATAGATTGCCGCCAGTCCCGCCTGGCTAAATCGAGACGGATCCTGCACGATTTTGTAGCTTTTACCGTCGCGCTGGTAATCCTGATTTGCCGTCGCGTTCAGTGGTTCGAAACGCGGGTCCGCCCCGCTAATTTCAACTATAGGGCCGTTACATACCGCAGGCTGCGGTACACTTACCGTCTGTTGCTGACCATCATCGCTTGTACATGCCGCGAGCATTCCTGCCGCGATGCAGATCCCGAGCCACTGCTTACGCATTGCGCACCTCTTACACGCTTTTCGACAACATTTTCCTGTGGGTGTGGATTGACATTACAATCCCGAACCCAGCCATCAGCACAATTAGCGCCGATCCTCCATAACTGACCAGTGGGAGCGGAACCCCTACAACCGGCAGAATACCGCT  >  NC_000913/664874‑665338
                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                            
aGCTGTGTCGGATCGAATGCTTCGCCAGAGGCCGTCAGGTTACTGCCGGGTTCGGCATCATAGATTGCCGCCAGTCCCGCCTGGCTAAATCGAGACGGATCCTGCACGATTTTGTAGCTTTTACCGTCGCGCTGGTAATCCTGATTTGCCGTCGCGTTCAGTGGTTCGAAACGCGGGTCCGCCCCGCTAATTTCAACTATAGGGCCGTTACATACCGCAGGCTGCGGTACACTTACCg                                                                                                                                                                                                                                       >  1:1009171/1‑238 (MQ=255)
     tgtCGGATCGAATGCTTCGCCAGAGGCCGTCAGGTTACTGCCGGGTTCGGCATCATAGATTGCCGCCAGTCCCGCCTGGCTAAATCGAGACGGATCCTGCACGATTTTGTAGCTTTTACCGTCGCGCTGGTAATCCTGATTTGCCGTCGCGTTCAGTGGTTCGAAACGCGGGTCCGCCCCGCTAATTTCAACTATAGGGCCGTTCCATACCGCAGGCTGCGGTACACTTACCGTCTGtt                                                                                                                                                                                                                                 >  1:558833/1‑239 (MQ=255)
     tgtCGGATCGAATGCTTCGCCAGAGGCCGTCAGGTTACTGCCGGGTTCGGCATCATAGATTGCCGCCAGTCCCGCCTGGCTAAATCGAGACGGATCCTGCACGATTTTGTAGCTTTTACCGTCGCGCTGGTAATCCTGATTTGCCGTCGCGTTCAGTGGTTCGAAACGCGGGTCCGCCCCGCTAATTTCAACTATAGGGCCGTTACATACCGCAGGCTGCGGTACACTTACCGTCTGtt                                                                                                                                                                                                                                 >  1:306225/1‑239 (MQ=255)
     tgtCGGATCGAATGCTTCGCCAGAGGCCGTCAGGTTACTGCCGGGTTCGGCATCATAGATTGCCGCCAGTCCCGCCTGGCTAAATCGAGACGGATCCTGCACGATTTTGTAGCTTTTACCGTCGCGCTGGTAATCCTGATTTGCCGTCGCGTTCAGTGGTTCGAAACGCGGGGCCGCCCCGCTAATTTCAACTTTAGGGCCGTTACATACCCCAGGCGGCGGGACCCTTACCGCCTGtt                                                                                                                                                                                                                                 >  2:854159/1‑239 (MQ=255)
     tgtCGGATCGAATGCTTCGCCAGAGGCCGTCAGGTTACTGCCGGGTTCGGCATCATAGATTGCCGCCAGTCCCGCCTGGCTAAATCGAGACGGATCCTGCACGATTTTGTAGCTTTTACCGTCGCGCTGGTAATCCTGATTTGCCGTCGCGTTCAGAGGTTCGAAACGCGGGTCAGCCCCGCTAATTTCAACTCTAGGGCCGTTAAAGACCCCAAGCTGCGGGACACTTACCGCTTGtt                                                                                                                                                                                                                                 >  2:266204/1‑239 (MQ=255)
       tCGGAGCGAATGCTTCCCCAGAGGCCGTGAGGTTGCGGCCGGGTTCGGCATCATAGATTGCCGCCAGTCCCGCCTGGCTAAATCGAGACGGATCCTGCACGATTTTGTAGCTTTTACCGTCGCGCTGGTAATCCTGATTTGCCGTCGCGTTCAGTGGTTCGAAACGCGGGTCCGCCCCGCTAATTTCAACTATAGGGCCGTTACATACCGCAGGCTGCGGTACACTTACCGTCTGTTg                                                                                                                                                                                                                                <  1:10206/238‑1 (MQ=255)
         ggttctattttttCGCCAGGGCCCGTCGGTTTCCGGCGGGGTTGGGCTCCATGGTGTCCCGCCAGTCCCGCCTGGCTAAATCGAGACGGATCCTGCACGATTTTGTAGCTTTTACCGTCGCGCTGGTAATCCTGATTTGCCGTCGCGTTCAGTGGTTCGAAACGCGGGTCCGCCCCGCTAATTTCAACTATAGGGCCGTTACATACCGCAGGCTGCGGTACACTTACCGTCTGTTGCt                                                                                                                                                                                                                              <  2:197268/227‑1 (MQ=255)
          gATCGAATGCTTCGCCAGAGGCCGTCAGGTTACTGCCGGGTTCGGCATCATAGATTGCCGCCAGTCCCGCCTGGCTAAATCGAGACGGATCCTGCACGATTTTGTAGCTTTTACCGTCGCGCTGGTAATCCTGATTTGCCGTCGCGTTCAGTGGTTCGAAACGCGGGGCCGCCACGCTAATTTCAACTATAGGGCCGTTACATACCGCAGGCTGCGGGAAACTTACCGTCTGTTGCCGa                                                                                                                                                                                                                            >  1:489374/1‑239 (MQ=255)
               aaTGCTTCGCCAGAGGCCGTCAGGTTACTGCCGGGTTCGGCATCATAGATTGCCGCCAGTCCCGCCTGGCTAAATCGAGACGGATCCTGCACGATTTTGTAGCTTTTACCGTCGCGCTGGTAATCCTGATTTGCCGTCGCGTTCAGTGGTTCGAAACGCGGGTCCGCCCCGCTAATTTCAACTATAGGGCCGTTACATACCGCAGGCTGCGGTACACTTACCGTCTGTTGCTGACcatc                                                                                                                                                                                                                       >  1:197657/1‑239 (MQ=255)
                atccctccccAGGGGCCGTAGGTTTCCGCCCGGGTTCGGAATCCAAGATTGCCGCCAGTCCCGGCTGGCTAAATCGAGACGGATCCTGCACGATTTTGTAGCTTTTACCGTCGCGCTGGTAATCCTGATTTGCCGTCGCGTTCAGTGGTTCGAAACGCGGGTCCGCCCCGCTAATTTCAACTATAGGGCCGTTACATACCGCAGGCTGCGGTACACTTACCGTCTGTTGCTGACcatc                                                                                                                                                                                                                       <  1:873386/230‑1 (MQ=255)
                                 gTCAGGTTACTGCCGGGTTCGGCATCATAGATTGCCGCCAGTCCCGCCTGGCTAAATCGAGACGGATCCTGCACGATTTTGTAGCTTTTACCGTCGCGCTGGTAATCCTGATTTGCCGTCGCGTTCAGTGGTTCGAAACGCGGGTCCGCCCCGCTAATTTCAACTATAGGGCCGTTACATACCCCAGGCTGCGGGACCCTTACCCTCTGTTGCTGGCCAACACCCCTTGTaaacaccgc                                                                                                                                                                                                     >  2:55843/1‑231 (MQ=255)
                                  tcgggTTACGGCCGGGTTGGGCTTCATAGATGGCCGCCAGTCCCGCCTGGCTAAATCGAGACGGATCCTGCACGATTTTGTAGCTTTTACCGTCGCGCTGGTAATCCTGATTTGCCGTCGCGTTCAGTGGTTCGAAACGCGGGTCCGCCCCGCTAATTTCAACTATAGGGCCGTTACATACCGCAGGCTGCGGTACACTTACCGTCTGTTGCTGACCATCATCGCTTGTACATGCcgcg                                                                                                                                                                                                    <  1:386181/236‑1 (MQ=255)
                                                 ggTTCGGCATCATAGATTGCCGCCAGTCCCGCCTGGCTAAATCGAGACGGATCCTGCATGATTTTGTAGCTTTTACCGTCGCGCTGGTAATCCTGATTTGCCGTCGCGTTCAGTGGTTCGAAACGCGGGTCCGCCCCGCTAATTTCAACTATAGGGCCGTTACATACCGCAGGCTGCGGTACACTTACCGTCTGTTGCTGACCATCATCGCTTGTACATGCCGCGAGCATTCCTGCcgc                                                                                                                                                                                     >  1:580603/1‑239 (MQ=255)
                                                        catcatAGTTGGCCCCAAGTCCCGCCTGGCTAAATCGAGACGGATCCTGCACGATTTTGTAGCTTTTACCGTCGCGCTGGTAATCCTGATTTGCCGTCGCGTTCAGTGGTTCGAAACGCGGGTCCGCCCCGCTAATTTCAACTATAGGGCCGTTACATACCGCAGGCTGCGGTACACTTACCGTCTGTTGCTGACCATCATCGCTTGTACATGCCGCGAGCATTCCTGCCGCGATGCa                                                                                                                                                                               <  1:854159/238‑1 (MQ=255)
                                                                  tGCCGCCAGTCCCGCCTGGCTAAATCGAGACGGATCCTGCACGATTTTGTAGCTTTTACCGTCGCGCTGGTAATCCTGATTTGCCGTCGCGTTCAGTGGTTCGAAACGCGGGTCCGCCCCGCTAATTTCAACTATAGTGCCGTTACATACCGCAGGCTGCGGTACACTTACCGTCTGTTGCTGACCATCATCGCTTGTACATGCCGCGAGCATTCCTGCCGCGATGCGGATCCCGAGc                                                                                                                                                                     >  1:1084573/1‑238 (MQ=255)
                                                                  tGCCGCCAGTCCCGCCTGGCTAAATCGAGACGGATCCTGCACGATTTTGTAGCTTTTACCGTCGCGCTGGTAATCCTGATTTGCCGTCGCGTTCAGTGGTTCGAAACGCGGGTCCGCCCCGCTAATTTCAACTATAGGGCCGTTACATACCGCAGGCTGCGGTACCCTTACCGTCTGTTGCTGACCATCATCGCTTGTACATGCCGCGAg                                                                                                                                                                                                  >  2:831643/1‑210 (MQ=255)
                                                                  tGCCGCCAGTCCCGCCTGGCTAAATCGAGACGGATCCTGCACGATTTTGTAGCTTTTACCGTCGCGCTGGTAATCCTGATTTGCCGTCGCGTTCAGTGGTTCGAAACGCGGGTCCGCCCCGCTAATTTCAACTATAGGGCCGTTACATACCGCAGGCTGCGGTACACTTACCGTCTGTTGCTGACCATCATCGCTTGTACATGCCGCGAg                                                                                                                                                                                                  <  1:831643/210‑1 (MQ=255)
                                                                  tGCCGCCAGTCCCGCCTGGCTAAATCGAGACGGATCCTGCACGATTTTGTAGCTTTTACCGTCGCGCTGGTAATCCTGATTTGCCGTCGCGTTCAGTGGTTCGAAACGCGGGTCCGCCCCGCTAATTTCAACTATAGGGCCGTTACATACCGCAGGCTGCGGTACACTTACCGTCTGTTGCTGACCATCATCGCTTGGACATGCCCCGGGCATTCCTCCCGCGGTGCAAATCCCGGGcc                                                                                                                                                                    >  2:719857/1‑239 (MQ=255)
                                                                  gccccccAGTCCCCCCTGGTTAAATCGAGACGGGTCCGGCCCGATTTTGTAGCTTTTACCGTCGCGCTGGTAATCCTGATTTGCCGTCGCGTTCAGTGGTCCGAAACGCGGGTCCGCCCCGCTAATTTCAACTATAGGGCCGTTACATACCGCAGGCTGCGGTACACTTACCGTCTGTTGCTGACCATCATCGCTTGTACATGCCGCGAGCATTCCTGCCGCGATGCAGATCCCGAGcc                                                                                                                                                                    <  2:197657/234‑1 (MQ=255)
                                                                    ccgccAGTCCCGCCTGGCTAAATCGAGACGGATCCTGCCCGATTTTGTAGCTTTTCCCGTCGCGCTGGTAATCCTGATTTGCCGTCGCGTTCAGTGGTTCGAAACGCGGGTCCGCCCCGCTAATTTCAACTATAGGGCCGTTACATACCGCAGGCTGCGGTACACTTACCGTCTGTTGCTGACCATCATCGCTTGTACATGCCGCGAGCATTCCTGCCGCGATGCAGATCCCGAGCCAc                                                                                                                                                                  <  1:719857/239‑1 (MQ=255)
                                                                    ccgccAGTCCCGCCTGGCTAAATCGAGACGGATCCTGCACGATTTTGTAGCTTTTACCGTCGCGCTGGTAATCCTGATTTGCCGTCGCGTTCAGTGGTTCGAAACGCGGGTCCGCCCCGCTAATTTCAACTATAGGGCCGTTACATACCGCAGGCTGCGGTACACTTACCGTCTGTTGCTGACCATCATCGCTTGTACATGCCGCGAGCATTCCTGCCGCGATGCAGATCCCGAGCCa                                                                                                                                                                   >  1:930112/1‑238 (MQ=255)
                                                                    cccccAGTCCCCCTGTGTTAATTGGAGGCGGTTCCTGCGCGTTTTTTTAGCTTTTACCGTCGCGCTGGTAATCCTGATTTGCCGTCGCGTTCAGTGGTTCGAAACGCGGGTCCGCCCCGCTAATTTCAACTATAGGGCCGTTACATACCGCAGGCTGCGGTACACTTACCGTCTGTTGCTGACCATCATCGCTTGTACATGCCGCGAGCATTCCTGCCGCGATGCAGATCCCGAGCCAc                                                                                                                                                                  <  2:21334/236‑1 (MQ=255)
                                                                     cgccAGTCCCGCCTGGCTAAATCGAGACGGATCCTGCACGATTTTGTAGCTTTTACCGTCGCGCTGGTAATCCTGATTTGCCGTCGCGTTCAGTGGTTCGAAACGCGGGTCCGCCCCGCTAATTTCACCTATAGGGCCGTTACATACCGCAGGCGGCGGGACACTTACCGTCTGTTGTTGCCCATCATGCCTTGTACCTGCCCCGCGCCATCCTCCCGCAATGCAGATcccctcaccc                                                                                                                                                                  >  2:1018620/1‑231 (MQ=255)
                                                                         aGTCCCGCCTGGCTAAATCGAGACGGATCCTGCACGATTTTGTAGCTTTTACCGTCGCGCTGGTAATCCTGATTTGCCGTCGCGTTCAGTGGTTCGAAACGCGGGTCCGCCCCGCTAATTTCAACTATAGGGCCGTTACATACCGCAGGCTGCGGTACACTTACCGTCTGTTGCTGACCATCATCCCTTGTACATGCCGCGGGCATTCCTGCCGCGAGGCAGATCCCGAGCCACTGCtt                                                                                                                                                             >  2:56704/1‑239 (MQ=255)
                                                                            cccGCCTGGCTAAATCGAGCCGGATCCTGCCGGATTTTGTAGCTTTTACCGTCGCGCTGGTAATCCTGATTTGCCGTCGCGTTCAGTGGTTCGAAACGCGGGTCCGCCCCGCTAATTTCAACTATAGGGCCGTTACATCCCGCAGGCTGCGGTACACTTACCGTCTGTTGCTGACCATCATCGCTTGTACATGCCGCGAGCATTCCTGCCGCGATGCAGATCCCGAGCCACTGCTTAc                                                                                                                                                           <  1:370826/238‑1 (MQ=255)
                                                                                   ggCTAAACGGGGACGGTTCCTGCTCGTTTTTGTATCTTTTACCGCCGCGCTGGTAATCCTGATTTGCCGTCGCGTTCAGTGGTCCGAAACGCGGGTCCGCCCCGCTAATTTCAACTATAGGGCCGTTACATACCGCAGGCTGCGGTACACTTACCGTCTGTTGCTGACCATCATCGCTTGTACATGCCGCGAGCATTCCTGCCGCGATGCAGATCCCGAGCCACTGCTTACGCATTgcg                                                                                                                                                   <  1:466659/239‑1 (MQ=255)
                                                                                    gtttAAATCGGTGCGGTTTCTGCCCGTTTTTGTCGTTTTTCCCGGCGCGCTGGTAATCCTGATTTCCCGTCGCTGTCAGTGGTTCGAAACGCGGGTCCCCCCCGCTAATTTCAACTATGGGGCCGTTACATACCGCAGGCTGCGGTACACTTACCGTCTGTTGCTGACCATCATCGCTTGTACATGCCGCGAGCATTCCTGCCGCGATGCAGATCCCGAGCCACTGCTTACGCATTgcg                                                                                                                                                   <  2:820485/236‑1 (MQ=255)
                                                                                       tAAAGCGAGACGGATCCTGCACGATTTTGTAGCTTTTACCGTCGCGCTGGTAATCCTGATTTGCCGTCGCGTTCAGTGGTTCGAAACGCGGGTCCGCCCCGCTAATTTCAACTATAGGGCCGTTACATACCGCAGGCTGCGGTACACTTACCGTCTGTTGCTGACCATCATCGCTTGTACATGCCGCGAGCATTCCTGCCGCGATGCAGATCCCGAGCCACTGCTTACGCATTGCGCAc                                                                                                                                               <  1:771307/239‑1 (MQ=255)
                                                                                                ccGGATCCTGCCGCTTTTTTGCGCTTTTACCGTCGCGCTGGTAATCCTGATTTGCCGTCGCGTTCAGTGGTTCGAAACCCGGGTCCGCCCCTCTAATTTCAACTATAGGGCCGTTACATACCGCAGGCTGCGGTACACTTACCGTCTGTTGCTGACCATCATCGCTTGTACATGCCGCGAGCATTCCTGCCGCGATGCAGATCCCGAGCCACTGCTTACGCATTGCGCACCTCTTaca                                                                                                                                       <  1:882122/237‑1 (MQ=255)
                                                                                                aCGGATCCTGCACGATTTTGTAGCTTTTACCGTCGCGCTGGTAATCCTGATTTGCCGTCGCGTTCAGTGGTTCGAAACGCGGGTCCGCCCCGCTAATTTCAACTATAGGGCCGTTACATACCGCAGGCTGCGGTACCATTACCGCCTGTTGCGGCCCACCATCGCTTGTACAGTCCCCGAGCATTCCTCCCCGCGTCCAGATCCCGACCCCCCGCTTCCCCATTGGGAACCCCttgcc                                                                                                                                       >  2:319664/1‑235 (MQ=255)
                                                                                                         gCACGATTTTGTAGCTTTTACCGTCGCGCTGGTAATCCTGATTTGCCGTCGCGTTCAGTGGTTCGAAACGCGGGTCCGCCCCGCTAATTTCAACTATAGGGCCGTTACATACCGCAGGCTGCGGTACACTTACCGTCTGTTGCTGACCATCATCGCTTGTACATGCCCCGAGCATTCCTGCCCCGATGCAGATCCCGAGCCACTGCTTACGCATTGCGCACCCCTTACACGCTTTTCGa                                                                                                                             >  1:430410/1‑239 (MQ=255)
                                                                                                              aTTTTGTAGCTTTTACCGTCGCGCTGGTAATCCTGATTTGCCGTCGCGTTCAGTGGTTCGAAACGCGGGTCCGCCCCGCTAATTTCAACTATAGGGCCGTTACATACCGCAGGCTGCGGTACACTTACCGTCTGTTGCTGACCATCATCg                                                                                                                                                                                                                   >  2:418338/1‑150 (MQ=255)
                                                                                                              aTTTTGTAGCTTTTACCGTCGCGCTGGTAATCCTGATTTGCCGTCGCGTTCAGTGGTTCGAAACGCGGGTCCGCCCCGCTAATTTCAACTATAGGGCCGTTACATACCGCAGGCTGCGGTACACTTACCGTCTGTTGCTGACCATCATCg                                                                                                                                                                                                                   <  1:418338/150‑1 (MQ=255)
                                                                                                               ttttGTAGCTTTTACCGTCGCGCTGGTAATCCTGATTTGCCGTCGCGTTCAGTGGTTCGAAACGCGGGTCCGCCCCGCTAATTTCAACTATAGGGCCGTTACATACCGCAGGCTGCGGTACACTTACCGTCTGTTGCTGACCATCATCGCTTGTACATGCCGCGGGCATTCCTGCCGCGATGCAGATCCCGAGCCACCCCTTACGCATTGCGCCCCCCTTAAACGCtttctaacaaaaa                                                                                                                       >  2:437608/1‑229 (MQ=255)
                                                                                                               ttttGTAGCTTTTACCGTCGCGCTGGTAATCCTGATTTGCCGTCGCGTTCAGTGGTTCGAAACGCGGGTCCGCCCCGCTAATTTCAACTATAGGGCCGTTACATACCGCAGGCTGCGGTACACTTACCGTCTGTTGCTGACCATCATCGCTTGTACATGCCGCGAGCATTCCTGCCGCGATGCAGATCCCGAGCCACTGCTTACGCATTGCGCACCCCTTACACGCTTTTCGGCAACAt                                                                                                                       >  1:552519/1‑239 (MQ=255)
                                                                                                                  tGTAGCTTTTACCGTCGCGCTGGTAATCCTGATTTGCCGTCGCGTTCAGTGGTTCGAAACGCGGGTCCGCCCCGCTAATTTCAACTATAGGGCCGTTACATACCGCAGGCTGCGGTACACTTACCGTCTGTTGCTGACCATCATCGCTTGTACATGCCGCGAGCATTCCTGCCGCGATGCAGATCCCGAGCCACTGCTTACGCATTGCGCACCTCTTACACGCTTTTCGACAACCttt                                                                                                                     >  1:557385/1‑238 (MQ=255)
                                                                                                                         ttttCCCGCCGCCTTTGTAATCCTTTTTTGCCTTCCGTTTCGGGGGTTCGAAACGGGGGTCCCCCCGCCTAATTGCAACTATAGGGCCGTTACATCCCGCAGGCGGCGGTACCCTTCCCGTCTGTTGCTGACCATCATCGCTTGTACATGCCGCGAGCATTCCTGCCGCGATGCAGATCCCGAGCCACTGCTTACGCATTGCGCACCTCTTACACGCTTTTCGACAACATTTTCCtgtg                                                                                                              <  2:557385/239‑1 (MQ=255)
                                                                                                                          tttACCGTCGCGCTGGTAATCCTGATTTGCCGTCGCGTTCAGTGGTTCGAAACGCGGGTCCGCCCCGCTAATTTCAACTATAGGGCCGTTACATACCGCAGGCTGCGGTACACTTACCGTCTGTTGCTGACCATCATCGCTTGTACATGCCGCGAGCATTCCTGCCGCGATGCAGATCCCGAGCCACTGCTTACGCATTGCGCACCTCTTACACGCTTTTCGACACCATTTTCCTGTgg                                                                                                             >  1:1080631/1‑239 (MQ=255)
                                                                                                                          tttACCGTCGCGCTGGTAATCCTGATTTGCCGTCGCGTTCAGTGGTTCGAAACGCGGGTCCGCCCCGCTAATTTCAACTATAGGGCCGTCACATACCGCAGGCTGCGGTACACTTACCGTCTGTTGCTGACCATCCTCGCTTGTACATTCCGCGAGCATTCCTGCCGCGATGCCGATCCCGAACCACTGCTTACGCCTGTGCACCCCCTTCCACCCTTTTCGAAAACATTTTCCTTTgg                                                                                                             >  2:80999/1‑239 (MQ=255)
                                                                                                                            tACCGTCGCGCTGGTAATCCTGATTTGCCGTCGCGTTCAGTGGTTCGAAACGCGGGTCCGCCCCGCTAATTTCAACTATAGGGCCGTTACATACCGCAGGCTGCGGTACACTTACCGTCTGTTGCTGACCATCATCGCTTGTACATGCCGCGAGCATTCCTGCCGCGATCCAGATCCCAAGCCACTGCTTACGCATTTCGCCCCCCTTCACCCCTTTTCGCCAACCTTTTCCTGTGGgt                                                                                                           >  2:949004/1‑239 (MQ=255)
                                                                                                                                 tCGCGCTGGTAATCCTGATTTGCCGTCGCGTTCAGTGGTTCGAAACGCGGGTCCGCCCCGCTAATTTCAACTATAGGGCCGTTACATACCGCAGGCTGCGGTACACTTACCGTCTGTTGCTGACCATCATCGCTTGTACATGCCGCGAGCATTCCTGCCGCGCTGCAGATCCCGACCCCCTGCTTACGCATTGCGCACCTCTTCCACGCTTTTCCCCACAATTTTCCTGTGGGGGGGGa                                                                                                      >  2:1072493/1‑239 (MQ=255)
                                                                                                                                  cgcgCTGGTAATCCTGATTTGCCGTCGCGTTCAGTGGTTCGAAACGCGGGTCCGCCCCGCTAATTTCAACTATAGGGCCGTTACATACCGCAGGCTGCGGTACACTTACCGTCTGTTGCTGACCATCATCGCTTGTACATGCCGCGAGCATTCCTGCCGCGATGCAGATCCCTACCCACTGCTTACGCCTTGCGCCCCTCTTac                                                                                                                                        >  1:794285/1‑204 (MQ=255)
                                                                                                                                   gggcTGGGAATCCTGTTTTGCCGCTGGGTTCGGGGGTTCGAAACGGGGGTCCGCCCCGCTAATTTCAACTATAGGGCCGTTACATCCCGCAGGCTGCGGTACACTTACCGTCTGTTGCTGACCATCATCGCTTGTACATGCCGCGAGCATTCCTGCCGCGATGCAGATCCCGAGCCACTGCTTACGCATTGCGCACCTCTTACACGCTTTTCGACAACATTTTCCTGTGGGTGTGGAtt                                                                                                    <  1:665025/237‑1 (MQ=255)
                                                                                                                                   gcccttgTAATCCTGATTTGCCGTCGCGTTCAGTGGTTCGAAACGCGGGTCCGCCCCGTTAATTTCAACTATAGGGCCGTTCCATCCCGCAGGCTGCGGTACCCTTACCGTCTGTTTCTGACCATCATCGCTTGTACATGCCGCGAGCATTCCTGCCGCGATGCAGATCCCGAGCCACTGCTTACGCATTGCGCACCTCTTACACGCTTTTCGACAACATTTTCCTGTGGGTGTGGAtt                                                                                                    <  1:266204/233‑1 (MQ=255)
                                                                                                                                    cgcTGGTAATCCTGATTTGCCGTCGCGTTCAGTGGTTCGAAACGCGGGTCCGCCCCGCTAATTTCAACTATAGGGCCGTTACATACCGCAGGCTGCGGTACACTTACCGTCTGTTGCTGACCATCATCGCTTGTACATGCCGCGAGCATTCCTGCcgc                                                                                                                                                                                     >  2:288673/1‑158 (MQ=255)
                                                                                                                                    cgcTGGTAATCCTGATTTGCCGTCGCGTTCAGTGGTTCGAAACGCGGGTCCGCCCCGCTAATTTCAACTATAGGGCCGTTACATACCGCAGGCTGCGGTACACTTACCGTCTGTTGCTGACCATCATCGCTTGTACATGCCGCGAGCATTCCTGCcgc                                                                                                                                                                                     <  1:288673/158‑1 (MQ=255)
                                                                                                                                                   tttcccaccccgTTCTGTGGTTCAAAACGTGGTCCCCCCCCGCTAATTTCAACTATAGGCCCTTTACATACCGCGGGCTTGGGTGCAGTTCCCGTGTGTCGCTCACTGCTTTCTCTTATACGGGCCGCGTGCATTCCTTCCAGGATGCAGATCCCCGGCCAATGCTATCGCATTGCGCACCTCTTACACGCTTTTCGACAACATTTGCCTGGGGGTGTGGATTGACATTACAATCCCGa                                                                                    <  2:264247/229‑1 (MQ=255)
                                                                                                                                                        cGTCGGGTTAAGGGGTTCGAAACGCGGGTCCCCCCCGCTAATTTCAACTATAGGGCCGTTACATACCGCAGGCTGCGGTACCCTTACCGTCTGTTGCTGACCATCATCGCTTGTACATGCCGCGAGCATTCCTGCCGCGATGCAGATCCCGAGCCACTGCTTACGCATTGCGCACCTCTTACACGCTTTTCGACAACATTTTCCTGTGGGTGTGGATTGACATTACAATCCCGAACCCa                                                                               <  1:949004/239‑1 (MQ=255)
                                                                                                                                                         gTCGCGTTCAGTGGTTCGAAACGCGGGTCCGCCCCGCTAATTTCAACTATAGGGCCGTTACATACCGCAGGCTGCGGTACACTTACCGTCTGTTGCTGACCATCATCGCTTGTACATTCCGCGAGCATTCCTGCCGCGATGCAGATCCCGAGCCACTGCTTACGCATTGCGCACCTCTTACACGCTTTTCGGCAACATTTTCCTTTGGGTTTGGATGGCCATTACAATCCCGAACCCa                                                                               >  1:576915/1‑238 (MQ=255)
                                                                                                                                                            gcgTTCAGTGGTTCGAAACGCGGGTCCGCCCCGCTAATTTCAACTATAGGGCCGTTACATACCGCAGGCTGCGGTACACTTACCGTCTGTTGCTGACCATCATCGCTTGTACATGCCGCGAGCATTCCTGCCGCGATGCAGATCCCGAGCCACTGCTTACGCATTGCGCACCTCTTACACGCTTTTCGACAACATTTTCCTGTGGGTGTGGATTGACATTACAATCCCGAACCCAGcc                                                                            >  1:1005538/1‑238 (MQ=255)
                                                                                                                                                                         cGAAACGCGGGTCCGCCCCGCTAATTTCAACTATAGGGCCGTTACATACCGCAGGCTGCGGTACACTTACCGTCTGTTGCTGACCATCATCGCTTGTACATGCCGCGAGCATTCCTGCCGCGATGCAGATCCCGAGCCACTGCTTACGCATTGCGCACCTCTTACACGCTTTTCGACAACATTTTCCTGTGGGTGTGGAtt                                                                                                    >  2:76844/1‑201 (MQ=255)
                                                                                                                                                                         cGAAACGCGGGTCCGCCCCGCTAATTTCAACTATAGGGCCGTTACATACCGCAGGCTGCGGTACACTTACCGTCTGTTGCTGACCATCATCGCTTGTACATGCCGCGAGCATTCCTGCCGCGATGCAGATCCCGAGCCACTGCTTACGCATTGCGCACCTCTTACACGCTTTTCGACAACATTTTCCTGTGGGTGTGGAtt                                                                                                    <  1:76844/201‑1 (MQ=255)
                                                                                                                                                                            accgggggTCCCCCCCCCTATTTTCAACTATAGGCCCGTTACCTACCGCGGGTTGCGGTACACTTCCCGTCTGTTGCTGACCATCATCGCTTGTACATGCCGCGAGCATTCCTGCCGCGATGCAGATCCCGAGCCACTGCTTACGCATTGCGCACCTCTTACACGCTTTTCGACAACATTTTCCTGTGGGTGTGGATTGACATTACAATCCCGAACCCAGCCATCAGCACAATTAgcgc                                                           <  2:1080631/234‑1 (MQ=255)
                                                                                                                                                                               gcgGGTCCGCCCCGCTAATTTCAACTATAGGGCCTTTACATCCCGCAGGCTGCGGTACACTTACCGTCTGTTGCTGACCATCATCGCTTGTACATGCCGCGAGCATTCCTGCCGCGATGCAGATCCCGAGCCACTGCTTACGCATTGCGCACCTCTTACACGCTTTTCGACAACATTTTCCTGTGGGTGTGGATTGACATTACAATCCCGAACCCAGCCATCAGCACAATTAGCGCCGa                                                        <  1:909471/239‑1 (MQ=255)
                                                                                                                                                                               gcgGGTCCCCCCCGCTAATTTCAACTATAGGGCCTTTACATACCGCAGGCTGCGGTACTCTTACCGTCTGTTGCTGACCATCATCGCTTGTACATGCCGCGAGCATTCCTGCCGCGATGCAGATCCCGAGCCACTGCTTACGCATTGCGCACCTCTTACACGCTTTTCGACAACATTTTCCTGTGGGTGTGGATTGACATTACAATCCCGAACCCAGCCATCAGCACAATTAGCGCCGa                                                        <  1:1072493/239‑1 (MQ=255)
                                                                                                                                                                               ccgGGTCCCCCCCTTTTATTTCAATTTTGGGGCCTTTCAACACCGCGGGGTGGGGTCCCCTTCCCGTCTGTTGCTGCCCATCATCGCTTGTCCATGCCGCGAGCATTCCTGCCGCGATGCAGATCCCGAGCCACTGCTTACGCATTGCGCACCTCTTACACGCTTTTCGACAACATTTTCCTGTGGGTGTGGATTGACATTACAATCCCGAACCCAGCCATCAGCACAATTAGCGCCGa                                                        <  2:430410/238‑1 (MQ=255)
                                                                                                                                                                               ccgGGTCCCCCCCGCTAATTCTAACTATAGGGCCGTTACATACCGCAGGCTGCGGTACACTTACCGTCTGTTGCTGACCATCATCGCTTGTACATGCCGCGAGCATTCCTGCCGCGATGCAGATCCCGAGCCACTGCTTACGCATTGCGCACCTCTTACACGCTTTTCGACAACATTTTCCTGTGGGTGTGGATTGACATTACAATCCCGAACCCAGCCATCAGCACAATTAGCGCCGa                                                        <  1:350336/238‑1 (MQ=255)
                                                                                                                                                                               ccgGGCCCCCCCCCCTAATTTCAACTTTAGGGCCGTTACTTCCCGCAGGCGGCGGTCCCCTTCCCTTCTGTTGCCGCCCATCACCCCTTTTACATGCCGCGAGCATTCCTGCCGCGATGCAGATCCCGAGCCACTGCTTACGCATTGCGCACCTCTTACACGCTTTTCGACAACATTTTCCTGTGGGTGTGGATTGACATTACAATCCCGAACCCAGCCATCAGCACAATTAGCGCCGa                                                        <  2:1005538/238‑1 (MQ=255)
                                                                                                                                                                                        ccccTCTAATTTCAACTATGGGGCCGTTACATACCGCAGGCTGCGGTACCCTTACCGTCTGTTGCTGACCATCATCGCTTGTACATGCCGCGAGCATTCCTGCCGCGATGCAGATCCCGAGCCACTGCTTACGCATTGCGCACCTCTTACACGCTTTTCGACAACATTTTCCTGTGGGTGTGGATTGACATTACAATCCCGAACCCAGCCATCAGCACAATTAGCGCCGATCCTCCATa                                               <  1:437608/239‑1 (MQ=255)
                                                                                                                                                                                               aaTTGCAACTAAGGGGCCGTTACCACCCGCAGGCTGGGGTACCTTTACCGTCTGTTGCTGGCCCACATCGCTTGTACATGCCGGCAGCATTCCTGCCGCGATGCAGATCCCGAGCCACTGCTTACGCATTGCGCACCTCTTACACGCTTTTCGACAACATTTTCCTGTGGGTGTGGATTGACATTACAATCCCGAACCCAGCCATCAGCACAATTAGCGCCGATCCTCCATAACTGAcc                                        <  1:550919/239‑1 (MQ=255)
                                                                                                                                                                                                  ttCAACTTTAGGGCCTTTTCATCCCCCAGGCTGCGGTACACTTCCCGTTTTTTGCTGACCATCATCGCTTGTACATGCCGCGAGCATTCCTGCCGGGATGCAGATCCCGAGCCACTGCTTACGCATTGCGCACCTCTTACACGCTTTTCGACAACATTTTCCTGTGGGTGTGGATTGACATTACAATCCCGAACCCAGCCATCAGCACAATTAGCGCCGATCCTCCATAACTGACCAGt                                     <  2:489374/239‑1 (MQ=255)
                                                                                                                                                                                                   ccAACTAGAGGGCCGTTACATACCGCAGGCTGCGGTACCCTTACCGTCTGTTGCTGACCATCATCGCTTGTACATGCCGCGAGCATTCCTGCCGCGATGCAGATCCCGAGCCACTGCTTACGCATTGCGCACCTCTTACACGCTTTTCGACAACATTTTCCTGTGGGTGTGGATTGACATTACAATCCCGAACCCAGCCATCAGCACAATTAGCGCCGATCCTCCATAACTGACCAGTg                                    <  1:55843/238‑1 (MQ=255)
                                                                                                                                                                                                           gggcccGTTCCCTCCCGCGGGCGGGGGTTCACTTACCCTCGTTGGAAGACCTACATCGCTTGTACTTGCCGCAGGCATTCCTGCCGCGATGCAGATCCCGGGCCACTGCTTACGCATTGCGCACCTCTTACGCGCTTTTCGACAACATTTTCCTGTCGGTGTGGATTGACATTACAATCCCGAACCCAGCCATCAGCACAATTAGCGCCGATCCTCCATAACTGACCAGTGGGAGCGGa                            <  2:70574/235‑1 (MQ=255)
                                                                                                                                                                                                           aGGCCCGTTACATGCCGCGGGCCGCGGTACACTTACCGTCTGTTGCTGACCATCCGTGCTTGTACATGCCGCGAGCATTCCTGCCGCGATGCAGATCCCGAGCCACTGCTTACGCATTGCGCACCTCTTACACGCTTTTCGACAACATTTTCCTGTGGGTGTGGATTGACATTACAATCCCGAACCCAGCCATCAGCACAATTAGCGCCGATCCTCCATAACTGACCAGTGGGAGCGGa                            <  1:1071703/239‑1 (MQ=255)
                                                                                                                                                                                                             ggCCGTTACATCCCGCAGGCTGCGGTACCCTTACCGTCTGTTGCTGACCATCACCGCTTGTACATGCCGCGAGCATTCCTGCCGCGATGCAGATCCCGAGCCACTGCTTACGCATTGCGCACCTCTTACACGCTTTTCGACAACATTTTCCTGTGGGTGTGGATTGACATTACAATCCCGAACCCAGCCATCAGCACAATTAGCGCCGATCCTCCATAACTGACCAGTGGGAGCGGa                            <  1:907450/237‑1 (MQ=255)
                                                                                                                                                                                                                  ttACATACCGCAGGCTGCGGTACACTTACCGTCTGTTGCTGACCATCATCGCTTGTACATGCCGCGAGCATTCCTGCCGCGATGCAGATCCCGAGCCACTGCTTACGCATTGCGCACCTCTTACACGCTTTTCGACAACATTTTCCTGTGGGTGTGGATTGACATTACAATCCCGAACCCAGCCATCAGCACAATTAgcg                                                            >  1:156980/1‑200 (MQ=255)
                                                                                                                                                                                                                  ttACATACCGCAGGCTGCGGTACACTTACCGTCTGTTGCTGACCATCATCGCTTGTACATGCCGCGAGCATTCCTGCCGCGATGCAGATCCCGAGCCACTGCTTACGCATTGCGCACCTCTTACACGCTTTTCGACAACATTTTCCTGTGGGTGTGGATTGACATTAAAATCCCGAACCCAGCCATCAGCACAATTAACGCCGATCCTCCCTAACTGACCAGTGGGAGCGGAAaccccc                     >  2:1039122/1‑237 (MQ=255)
                                                                                                                                                                                                                  ttACATACCGCAGGCTGCGGTACACTTACCGTCTGTTGCTGACCATCATCGCTTGTACATGCCGCGAGCATTCCTGCCGCGATGCAGATCCCGAGCCACTGCTTACGCATTGCGCACCTCTTACACGCTTTTCGACAACATTTTCCTGCGGGTGTGGATTGACATTACAATCCCGAACCCAGCCATCAGCACCATTATCGCCGAGCCTCCATAACTGACCAGTGGAACCGGAACCCCt                      >  1:100957/1‑238 (MQ=255)
                                                                                                                                                                                                                  tcaCATACCGCAGGCTGCGGTACACTTACCGTCTGTTGCTGACCATCATCGCTTGTACATGCCGCGAGCATTCCTGCCGCGATGCAGATCCCGAGCCACTGCTTACGCATTGCGCACCTCTTACACGCTTTTCGACAACATTTTCCTGTGGGTTTGGATTGACATTACAATCCCGAACCCAGCCATCAGCACAATTACCGCCGATCCCCCATAACTTACCACGGGGAGGGGAAccccaa                     >  2:761778/3‑237 (MQ=255)
                                                                                                                                                                                                                            cAGGCTGCGGTACACTTACCGTCTGTTGCTGACCATCATCGCTTGTACATGCCGCGAGCATTCCTGCCGCGATGCAGATCCCGAGCCACTGCTTACGCATTGCGCACCTCTTACACGCTTTTCGACAACATTTTCCTGTGGGTGTGGATTGACATTACAATCCCGAACCCa                                                                               >  2:59643/1‑171 (MQ=255)
                                                                                                                                                                                                                            cAGGCTGCGGTACACTTACCGTCTGTTGCTGACCATCATCGCTTGTACATGCCGCGAGCATTCCTGCCGCGATGCAGATCCCGAGCCACTGCTTACGCATTGCGCACCTCTTACACGCTTTTCGACAACATTTTCCTGTGGGTGTGGATTGACATTACAATCCCGAACCCa                                                                               <  1:59643/171‑1 (MQ=255)
                                                                                                                                                                                                                            cAGGCTGCGGTACACTTACCGTCTGTTGCTGACCATCATCGCTTGTACATGCCGCGAGCATTCCTGCCGCGATGCAGATCCCGAGCCACTGCTTACGCATTGCGCACCTCTTACACGCTTTTCGACAACATTTTCCTGTGGGTGTGGATTGACATTACAATCCCGAACCCAGCCATCCGCCCACTTAGCGCCGATCCTCCATAACTGACCAGGGGGCGCGGAACCCCTAAAACCGGAAg           >  2:726346/1‑239 (MQ=255)
                                                                                                                                                                                                                            cAGGCTGCGGTACACTTACCGTCTGTTGCTGACCATCATCGCTTGTACATGCCGCGAGCATTCCTGCCGCGATGCAGATCCCGAGCCACTGCTTACGCATTGCGCACCTCTTACACGCTTTTCGACAACATTTTCCTGTGGGTGTGGATTGACATTACAATCCCGAACCCAGCCATCAGCACAATTAGCGCCGATCCTCCATAACTGACCAGTGGGAGCGGAAACCCTACAACCGGccg           >  1:582347/1‑237 (MQ=255)
                                                                                                                                                                                                                              tgcctcGGTACCCTTACCGTCGTGTGCTGACCATCACCGCTTGTACATGCCGCGATCTTTCCTGCCGTGATGCAGATCCCGAGCCACTGCTTACGCATTGCGCACCTCTTACACGCTTTTCGACAACATTTTCCTGTGGGTGTGGATTGACATTACAATCCCGAACCCAGCCATCAGCACAATTAGCGCCGATCCTCCATAACTGACCAGTGGGAGCGGAACCCCTACAACCGGCAGaa         <  1:902022/234‑1 (MQ=255)
                                                                                                                                                                                                                              ggCTGGGTTACACTTCCCGTTTTGTGCGGCCCATTCTCGCTTTGCCATGCCGCGAGCATTCCGGCCGCGATGCAGATCCCGAGCCACTGCTTACGCATTGCGCACCTCGTACCCGCTTTTCGACAACATTTTCCTGGGGGTGTGGATTGACATTACAATCCCGAACCCAGCCATCAGCACAATTAGCGCCGATCCTCCATAACTGACCAGTGGGAGCGGAACCCCTACAACCGGCAGaa         <  2:306225/239‑1 (MQ=255)
                                                                                                                                                                                                                               gctgggttccacTTGCCTTCTGTTGCTGACCAGCATCGCTTGTACATGCCCGGGACATTCCTGCCGCGATGCAGATCCCGAGCCACTGCTTACGCATTGCGCACCGCTTACACGCTTTTCGACAACATTTTCCTGTGGGTGTGGATTGACATTACAATCCCGAACCCAGCCATCAGCACAATTAGCGCCGATCCTCCATAACTGACCAGTGGGAGCGGAACCCCTACAACCGGCAGaa         <  1:319664/229‑1 (MQ=255)
                                                                                                                                                                                                                                      tACCCTTACCTTCTGTTGCTGACCATCATCGCTTGTACATGCCGCGAGCATTCCTGCCGGGATGCAGATCCCGAGCCACTGCTTACGCATTGCGCACCTCTTACACGCTTTTCGACAACATTTTCCTGTGGGTGTGGATTGACATTACAATCCCGAACCCAGCCATCAGCACAATTAGCGCCGATCCTCCATAACTGACCAGTGGGAGCGGAACCCCTACAACCGGCAGAATACCGCt  <  1:774192/238‑1 (MQ=255)
                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                            
AGCTGTGTCGGATCGAATGCTTCGCCAGAGGCCGTCAGGTTACTGCCGGGTTCGGCATCATAGATTGCCGCCAGTCCCGCCTGGCTAAATCGAGACGGATCCTGCACGATTTTGTAGCTTTTACCGTCGCGCTGGTAATCCTGATTTGCCGTCGCGTTCAGTGGTTCGAAACGCGGGTCCGCCCCGCTAATTTCAACTATAGGGCCGTTACATACCGCAGGCTGCGGTACACTTACCGTCTGTTGCTGACCATCATCGCTTGTACATGCCGCGAGCATTCCTGCCGCGATGCAGATCCCGAGCCACTGCTTACGCATTGCGCACCTCTTACACGCTTTTCGACAACATTTTCCTGTGGGTGTGGATTGACATTACAATCCCGAACCCAGCCATCAGCACAATTAGCGCCGATCCTCCATAACTGACCAGTGGGAGCGGAACCCCTACAACCGGCAGAATACCGCT  >  NC_000913/664874‑665338

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: