| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 811,721 | T→G | 46.8% | V67G (GTG→GGG) | bioC → | malonyl‑ACP O‑methyltransferase, SAM‑dependent |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 811,721 | 0 | T | G | 46.8% | 15.2 / 24.1 | 23 | V67G (GTG→GGG) | bioC | malonyl‑ACP O‑methyltransferase, SAM‑dependent |
| Reads supporting (aligned to +/- strand): ref base T (6/5); new base G (10/2); total (16/7) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.93e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.66e-03 | |||||||||||
ATGCAGGATATCGACCGTCTGCTGGAGGTGCTGCATGGCAACGGTTAATAAACAAGCCATTGCAGCGGCATTTGGTCGGGCAGCCGCACACTATGAGCAACATGCAGATCTACAGCGCCAGAGTGCTGACGCCTTACTG‑GCAATGCTTCCACAGCGTAAATACACCCACGTACTGGACGCGGGTTGTGGACCTGGCTGGATGAGCCGCCACTGGC‑GGGAACGTCACGCGCAGGTGACGGCCTTAGATCTCTCGCCGCCAATGCTTGTTCAGGCACGCCAGAAGGATGCCGCAGACCATTATCTGGCGGGAGATATCGAATCCCTG‑CCGTTAGCGACTGCG‑ACGTTCGATCTTGCATGGAGCAATCTCGCAGTGCAGTGGTGCGGT‑AATTTATCCACGGCACTCCGCGAGCTGTATCGGGTGGTGCGCCCCAAAGGCGTGGTCGCGTTTACCACGCTGGTGCAGGGATCGT > NC_000913/811488‑811957 | aTGCAGGATATCGACCGTCTGCTGGAGGTGCTGCATGGCAACGGTTAATAAACAAGCCATTGCAGCGGCATTTGGTCGGGCAGCCGCACACTATGAGCAACATGCAGATCTACAGCGCCAGAGTGCTGACGCCTTACTG‑GCAATGCTTCCACAGCGTAAATACACCCACGTACTGGACGCGGGTTGTGGACCTGGCTGGATGAGCCGCCACTGGCGGGGAACG‑CACGCGCACGGGACg > 1:1080867/1‑238 (MQ=255) gTGCTGCATGGCAACGGTTAATAAACAAGCCATTGCAGCGGCATTTGGTCGGGCAGCCGCACACTATGAGCAACATGCAGATCTACAGCGCCAGAGTGCTGACGCCTTACTG‑GCAATGCTTCCACAGCGTAAATACACCCACGTACTGGACGCGGGTTGTGGACCTGGCTGGATGAGCCGCCACTGGC‑GGGAACGTCACGCGCAGGGGACGGCCCTAGATCTCTCGCCGCCCATGCTTg > 1:413104/1‑239 (MQ=255) gTTAATAAACAAGCCATTGCAGCGGCATTTGGTCGGGCAGCCGCACACTATGAGCAACATGCAGATCTACAGCGCCAGAGTGCTGACGCCTTACTG‑GCAATGCTTCCACAGCGTAAATACACCCACGTACTGGACGCGGGTTGTGGACCTGGCTGGATGAGCCGCCACTGGC‑GGGAACGGCCCGCCCCGGGGACGGCCCTAGATCTCCCGCCGCCCAGGCGTGTTCAGGCACCCCCGaa > 2:756714/1‑239 (MQ=255) ttAAAAAACCACCCATGGCGGGGGCTTTTGGGCGGGCCACCGCACCCTAGGAGCACCTTGAAGTGTTATAGCCCCAGGGGGCT‑CGGCCTTTCCGTACAATGCTTCCACAGCATAAATACCCCCACTTACGGGGGGCGGGTTGGGGACCTGGTTGTATGAGCCGCCACTGGC‑GGGAACGTCACGCGCAGGTGACGGCCTTAGATCTCTCGCCGCCAATGCTTGTTCAGGCACGTCAGaa < 2:363507/238‑1 (MQ=255) atgCCATTGCCGGGGTGTTTGGGCGGGCGAGCGCACACTGTGAACAACAGGCAAATCTACGGCGCCAGGGTTCGGACGCCTTGCGG‑GAAATGCTTCCACGGGTTAAATACCCCCCGGTCTGGGACGGGGGTTGTGGCCCGGGCTGGATGGGCCGCCACTGGC‑GGGAACGTCACGCGCAGGTGACGGCCTTAGATCTCTCGCCGCCAATGCTTGTTCAGGCACGCCAGAAGGATGCCGCa < 2:125356/237‑1 (MQ=255) aaGCCTTTGCAGCGGCATTTGGTCGGGCAGCCGCACACTATGAGCAACATGCAGATCTACAGCGCCAGAGTGCTGACGCCTTACTG‑GCAATGCTTCCACAGCGTAAATCCACCCACGTACTGGACGCGGGTTGTGGCCCTGGCTGGATGAGCCGCCACTGGC‑GGGAACGTCACGCGCAGGTGACGGCCTTAGATCTCTCGCCGCCAATGCTTGTTCAGGCACGCCAGAAGGATGCCGCa < 1:212095/239‑1 (MQ=255) aGCCATTGCAGCGGCATTTGGTCGGGCAGCCGCACACTATGAGCAACATGCAGATCTACAGCGCCAGAGTGCTGACGCCTTACTG‑GCAATGCTTCCACAGCGTAAATACACCCACGTACTGGACGCGGGTTGTGGACCTGGCTGGATGAGCCGCCACTGGC‑GGGGACGGCGCCCGCACGGGAGAGCCCTCGATCCCTCCCCGCCCCACCGTTTTCAGGCCCCCCAGAAGGAAGCCGcg > 2:849569/1‑237 (MQ=255) aGCCATTGCAGCGGCATTTGGTCGGGCAGCCGCACACTATGAGCAACATGCAGATCTACAGCGCCAGAGTGCTGACGCCTTACTG‑GCAATGCTTCCACAGCGTAAATACACCCACGTACTGGACGCGGGTTGTGGACCTGGCTGGATGAGCCGCCACTGGC‑GGGAACGCCACACCCAGGGGACGGCCTTAGATTTCTCGCCGCCACAGCTTGTTTAGGCCACCCAGCAGGATGccccgg > 1:1072977/1‑235 (MQ=255) tGAGCAACATGCAGATCTACAGCGCCAGAGTGCTGACGCCTTACTG‑GCAATGCTTCCACAGCGTAAATACACCCACGTACTGGACGCGGGTTGTGGACCTGGCTGGATGAGCCGCCACTGGC‑GGGGACGTCCCGCGCAAGGGGCGGCCGTAGATCTCCCGCCCCCCAAGCTTTGTCAGGCCCGACCGAAGGAAGGCCCCGCACATTATCAGGCGGGGGAAATCCGAACCCCG‑CCGCTac > 2:660908/1‑238 (MQ=255) aCATGCAGATCTACAGCGCCAGAGTGCTGACGCCTTACTG‑GCAATGCTTCCACAGCGTAAATACACCCACGTACTGGACGCGGGTTGTGGACCTGGCTGGATGAGCCGCCACTGGC‑GGGAACGTCACGCGCCGGTGACGGCCTTAGATCTCTCGCCGCCCATGCTTGTTCAGGCACGCCCGAAGGCAGCCGCAGACCCTTATCTTGCGGGCGATATCGAAACCCTG‑CCCTTAGCGAca > 1:739444/1‑237 (MQ=255) aCATGCAGATCTACAGCGCCAGAGTGCTGACGCCTTACTG‑GCAATGCTTCCACAGCGTAAATACACCCACGTACTGGACGCGGGTTGTGGACCTGGCTGGATGAGCCGCCACTGGC‑GGGAACGTCACGCGCAGGTGACGGCCTTAGATCTCTCGCCGCCAATGCTTGTTCAGGCACGCCCGAAAGATGCCGCAGACCCTTTTCCTGCGGGGGATATCGGATCCCTC‑CCGTTAGCGACTg > 1:328008/1‑239 (MQ=255) aCAGCCCCGGAGTTCTGACCCCTTACTG‑ACAAGGCTCCCACAGCGTAAATACCCCCACGTACTGGCCGCGGGTTGTGGACCTGGCGGGATGAGCCGCCACTGGC‑GGGAACGTCACGCGCAGGTGACGGCCTTAGATCTCTCGCCGCCAATGCTTGTTCAGGCACGCCAGAAGGATGCCGCAGACCATTATCTGGCGGGAGATATCGAATCCCTG‑CCGTTAGCGACTGCG‑ACGTTCGATc < 1:600848/239‑1 (MQ=255) ccAGAGTGCTGACGCCTTACTG‑GCAATGCTTCCACAGCGTAAATACACCCACGTACTGGACGCGGGTTGTGGACCTGGCTGGATGAGCCGCCACTGGC‑GGGAACGTCACGCGCAGGTGACGGCCTTAGATCTCTCGCCGCCAATGCTTGTTTCGGCACGCCAGAAGGATGCCGCAGACCATTATCTGGCGGGAGATATCGAATCCCTC‑CCGTTAGCGGCTGCG‑ACGGTCGCTCttt > 1:977581/1‑235 (MQ=255) aCGCCTTACTG‑GCAATGCTTCCACAGCGTAAATACACCCACGTACTGGACGCGGGTTGTGGACCTGGCTGGATGAGCCGCCACTGGC‑GGGAACGTCACGCGCAGGGGACGGCCTTAGATCTCTCGCCGCCAATGCTTGGTCAGGCACCCCCGAAGGATGCCGCAGACCATTATCTTGCGGGGGATATCGAATCCCTG‑CCGGTTGGGGCTGCG‑ACGTTTGATCTTGCCTGGGGCaaatt > 1:755786/1‑235 (MQ=255) tAAATACACCCACGTACTGGACGCGGGTTGTGGACCTGGCTGGATGAGCCGCCACTGGC‑GGGAACGTCACGCGCAGGTGACGGCCTTAGATCTCTCGCCGCCAATGCTTGTTCAGGCACGCCAGAAGGATGCCGCAGACCCTTATCTTGCGGGAGATATCGAATCCCTG‑CCGCTAGCGACTGCG‑ACGTTCGATCTTTCATGGGGCAAACTCGCAGTGCAGTGGGGCGGG‑AAATTTTcc > 1:337287/1‑238 (MQ=255) tAAATACACCCACGTACTGGACGCGGGTTGTGGACCTGGCTGGATGAGCCGCCACTGGC‑GGGAACGCCACGCGCAGGGGACGGCCTTTGATCTCTCGCCCCCCATGCTTGTTTCGGCCCCCCAGAAGGATGCCCCAGACCCTTTTCTGCCGGGGGCAATCGGACCCCCC‑CCGTTAGCGCCGGCG‑CCGCTCCTTCTTGCGTGGGGCAAACTCCCCCGGCAGGGGGGGGGG‑AATTTAttc > 2:746089/1‑236 (MQ=255) cccACGTACTGGACGCGGGTTGTGGACCTGGCTGGATGAGCCGCCACTGGC‑GGGAACGTCACGCGCAGGTGACGGCCTTAGATCTCTCGCCGCCAATGCTTGGTCAGGCACGCCCGAAGGATGCCGCAGACCCTTATCTGGCGGGGGGTATCGAATCCCTG‑CCGTTa > 1:61429/1‑167 (MQ=255) gTAGTGGGCGGGGGTTGGGGCCTGGGCTGGATGGCCCGCCACGGGC‑GGAAACGTCACGCGCAGGTGACGGCCTTAGATCTCTCGCCGCCAATGCTGGTTCAGGCACGCCAGAAGGATGCCGCAGACCATTATCTGGCGGGAGATATCGAATCCCTG‑CCGTTAGCGACTGCG‑ACGTTCGATCTTGCATGGAGCAATCTCGCAGTGCAGTGGTGCGGT‑AATTTATCCACGGCACTCCGCGa < 1:849569/239‑1 (MQ=255) gccggggggggggTTGGGGCCGTGGTGGGATGGGCCGCCCCGGGC‑GGGAACGTCCGGCGAAGGGGACGGCCTTAGATCTCCCGCCGCAAATGCTTGTTCAGGCCCGCCAGAAGGATGCCGCAGACCATTATCTGGCGGGAGATATCGAATCCCTG‑CCGTTAGCGACTGCG‑ACGTTCGATCTTGCATGGAGCAATCTCGCAGTGCAGTGGTGCGGT‑AATTTATCCACGGCACTCCGCGAg < 1:642578/229‑1 (MQ=255) gtgGACCTGGCTGGATGAGCCGCCACTGGC‑GGGAACGTCACGCGGAGGGGACGGCCCTAGATCTCTCGCCGCCAATGCTTTGTCCGGGACGCCACAAGGAATCCGCAGACCATTTTCTGGCGGGGGGAATCGAACCCCCGCCCGTTGGCG‑CTCCGCACGTCCG‑TCTTTGCTGGGGCAATATCCCAGGGCAGGGGGGGGGG‑GAATTTTCCACCGCCCCCCCCGAGCTGGGTCgggggggg > 2:939461/1‑237 (MQ=255) ccgccACTGGC‑GGGAACGTCACGCGCAGGGGACGGCCTTAGATCTCTCGCCGCCCATGCTTGTTCAGGCACGCCCGAAGGATGGCGCAGACCCTTATCTGGCGGGGGATATTCGATCCCCT‑CCGGTTGCGGCCGCG‑ACGTTCGGTCTTTCCTTGGGCAATCTCCCCGGGCCGGGGGGCGGTGAATTTTCCCAC‑GCACCCCCCGAGCCGTAGCGGGGGGGGGGGCCCaaaagggggggcg > 2:261796/1‑229 (MQ=255) cACGGGC‑GGGAACGTCGGCGGCGGGGCGCGGCCTGAGATCTTCCGCCGCCAATGCTTGTTCAGGCACGCCAGAAGGGGTCCGCAGCCCATTATCGGGCGGGAGATATCGAATCCATG‑CCGTTAGCGACTGCG‑ACGTTCGATCTTGCATGGAGCAATCTCGCAGTGCAGTGGTGCGGT‑AATTTATCCACGGCACTCCGCGAGCTGTATCGGGTGGTGCGCCCCAAAGGCGTGGTCGCGtt < 2:1072977/239‑1 (MQ=255) ggTGACGGCCTTAGATCTCTCGCCGCCAATGCTTGTTCAGGCACGCCAGAAGGATGCCGCAGACCATTATCTGGCGGGAGATATCGAATCCCTG‑CCGTTAGCGACTGCG‑ACGTTCGATCTTGCATGGAGCAATCTCGCAGTGCAGTGGTGCGGT‑AATTTATCCACGGCACTCCGCGAGCTGTATCGGGGGGGGGGCCCCAAAGGGGTGGTCGCGTTTACCACCCTGGGGCgggggtggt > 2:577932/1‑234 (MQ=255) | ATGCAGGATATCGACCGTCTGCTGGAGGTGCTGCATGGCAACGGTTAATAAACAAGCCATTGCAGCGGCATTTGGTCGGGCAGCCGCACACTATGAGCAACATGCAGATCTACAGCGCCAGAGTGCTGACGCCTTACTG‑GCAATGCTTCCACAGCGTAAATACACCCACGTACTGGACGCGGGTTGTGGACCTGGCTGGATGAGCCGCCACTGGC‑GGGAACGTCACGCGCAGGTGACGGCCTTAGATCTCTCGCCGCCAATGCTTGTTCAGGCACGCCAGAAGGATGCCGCAGACCATTATCTGGCGGGAGATATCGAATCCCTG‑CCGTTAGCGACTGCG‑ACGTTCGATCTTGCATGGAGCAATCTCGCAGTGCAGTGGTGCGGT‑AATTTATCCACGGCACTCCGCGAGCTGTATCGGGTGGTGCGCCCCAAAGGCGTGGTCGCGTTTACCACGCTGGTGCAGGGATCGT > NC_000913/811488‑811957 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |