Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 811,721 T→G 46.8% V67G (GTG→GGG)  bioC → malonyl‑ACP O‑methyltransferase, SAM‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913811,7210TG46.8% 15.2 / 24.1 23V67G (GTG→GGG) bioCmalonyl‑ACP O‑methyltransferase, SAM‑dependent
Reads supporting (aligned to +/- strand):  ref base T (6/5);  new base G (10/2);  total (16/7)
Fisher's exact test for biased strand distribution p-value = 1.93e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.66e-03

ATGCAGGATATCGACCGTCTGCTGGAGGTGCTGCATGGCAACGGTTAATAAACAAGCCATTGCAGCGGCATTTGGTCGGGCAGCCGCACACTATGAGCAACATGCAGATCTACAGCGCCAGAGTGCTGACGCCTTACTGGCAATGCTTCCACAGCGTAAATACACCCACGTACTGGACGCGGGTTGTGGACCTGGCTGGATGAGCCGCCACTGGCGGGAACGTCACGCGCAGGTGACGGCCTTAGATCTCTCGCCGCCAATGCTTGTTCAGGCACGCCAGAAGGATGCCGCAGACCATTATCTGGCGGGAGATATCGAATCCCTGCCGTTAGCGACTGCGACGTTCGATCTTGCATGGAGCAATCTCGCAGTGCAGTGGTGCGGTAATTTATCCACGGCACTCCGCGAGCTGTATCGGGTGGTGCGCCCCAAAGGCGTGGTCGCGTTTACCACGCTGGTGCAGGGATCGT  >  NC_000913/811488‑811957
                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                               
aTGCAGGATATCGACCGTCTGCTGGAGGTGCTGCATGGCAACGGTTAATAAACAAGCCATTGCAGCGGCATTTGGTCGGGCAGCCGCACACTATGAGCAACATGCAGATCTACAGCGCCAGAGTGCTGACGCCTTACTGGCAATGCTTCCACAGCGTAAATACACCCACGTACTGGACGCGGGTTGTGGACCTGGCTGGATGAGCCGCCACTGGCGGGGAACGCACGCGCACGGGACg                                                                                                                                                                                                                                             >  1:1080867/1‑238 (MQ=255)
                           gTGCTGCATGGCAACGGTTAATAAACAAGCCATTGCAGCGGCATTTGGTCGGGCAGCCGCACACTATGAGCAACATGCAGATCTACAGCGCCAGAGTGCTGACGCCTTACTGGCAATGCTTCCACAGCGTAAATACACCCACGTACTGGACGCGGGTTGTGGACCTGGCTGGATGAGCCGCCACTGGCGGGAACGTCACGCGCAGGGGACGGCCCTAGATCTCTCGCCGCCCATGCTTg                                                                                                                                                                                                                 >  1:413104/1‑239 (MQ=255)
                                           gTTAATAAACAAGCCATTGCAGCGGCATTTGGTCGGGCAGCCGCACACTATGAGCAACATGCAGATCTACAGCGCCAGAGTGCTGACGCCTTACTGGCAATGCTTCCACAGCGTAAATACACCCACGTACTGGACGCGGGTTGTGGACCTGGCTGGATGAGCCGCCACTGGCGGGAACGGCCCGCCCCGGGGACGGCCCTAGATCTCCCGCCGCCCAGGCGTGTTCAGGCACCCCCGaa                                                                                                                                                                                                 >  2:756714/1‑239 (MQ=255)
                                            ttAAAAAACCACCCATGGCGGGGGCTTTTGGGCGGGCCACCGCACCCTAGGAGCACCTTGAAGTGTTATAGCCCCAGGGGGCTCGGCCTTTCCGTACAATGCTTCCACAGCATAAATACCCCCACTTACGGGGGGCGGGTTGGGGACCTGGTTGTATGAGCCGCCACTGGCGGGAACGTCACGCGCAGGTGACGGCCTTAGATCTCTCGCCGCCAATGCTTGTTCAGGCACGTCAGaa                                                                                                                                                                                                 <  2:363507/238‑1 (MQ=255)
                                                     atgCCATTGCCGGGGTGTTTGGGCGGGCGAGCGCACACTGTGAACAACAGGCAAATCTACGGCGCCAGGGTTCGGACGCCTTGCGGGAAATGCTTCCACGGGTTAAATACCCCCCGGTCTGGGACGGGGGTTGTGGCCCGGGCTGGATGGGCCGCCACTGGCGGGAACGTCACGCGCAGGTGACGGCCTTAGATCTCTCGCCGCCAATGCTTGTTCAGGCACGCCAGAAGGATGCCGCa                                                                                                                                                                                       <  2:125356/237‑1 (MQ=255)
                                                     aaGCCTTTGCAGCGGCATTTGGTCGGGCAGCCGCACACTATGAGCAACATGCAGATCTACAGCGCCAGAGTGCTGACGCCTTACTGGCAATGCTTCCACAGCGTAAATCCACCCACGTACTGGACGCGGGTTGTGGCCCTGGCTGGATGAGCCGCCACTGGCGGGAACGTCACGCGCAGGTGACGGCCTTAGATCTCTCGCCGCCAATGCTTGTTCAGGCACGCCAGAAGGATGCCGCa                                                                                                                                                                                       <  1:212095/239‑1 (MQ=255)
                                                      aGCCATTGCAGCGGCATTTGGTCGGGCAGCCGCACACTATGAGCAACATGCAGATCTACAGCGCCAGAGTGCTGACGCCTTACTGGCAATGCTTCCACAGCGTAAATACACCCACGTACTGGACGCGGGTTGTGGACCTGGCTGGATGAGCCGCCACTGGCGGGGACGGCGCCCGCACGGGAGAGCCCTCGATCCCTCCCCGCCCCACCGTTTTCAGGCCCCCCAGAAGGAAGCCGcg                                                                                                                                                                                       >  2:849569/1‑237 (MQ=255)
                                                      aGCCATTGCAGCGGCATTTGGTCGGGCAGCCGCACACTATGAGCAACATGCAGATCTACAGCGCCAGAGTGCTGACGCCTTACTGGCAATGCTTCCACAGCGTAAATACACCCACGTACTGGACGCGGGTTGTGGACCTGGCTGGATGAGCCGCCACTGGCGGGAACGCCACACCCAGGGGACGGCCTTAGATTTCTCGCCGCCACAGCTTGTTTAGGCCACCCAGCAGGATGccccgg                                                                                                                                                                                      >  1:1072977/1‑235 (MQ=255)
                                                                                             tGAGCAACATGCAGATCTACAGCGCCAGAGTGCTGACGCCTTACTGGCAATGCTTCCACAGCGTAAATACACCCACGTACTGGACGCGGGTTGTGGACCTGGCTGGATGAGCCGCCACTGGCGGGGACGTCCCGCGCAAGGGGCGGCCGTAGATCTCCCGCCCCCCAAGCTTTGTCAGGCCCGACCGAAGGAAGGCCCCGCACATTATCAGGCGGGGGAAATCCGAACCCCGCCGCTac                                                                                                                                              >  2:660908/1‑238 (MQ=255)
                                                                                                   aCATGCAGATCTACAGCGCCAGAGTGCTGACGCCTTACTGGCAATGCTTCCACAGCGTAAATACACCCACGTACTGGACGCGGGTTGTGGACCTGGCTGGATGAGCCGCCACTGGCGGGAACGTCACGCGCCGGTGACGGCCTTAGATCTCTCGCCGCCCATGCTTGTTCAGGCACGCCCGAAGGCAGCCGCAGACCCTTATCTTGCGGGCGATATCGAAACCCTGCCCTTAGCGAca                                                                                                                                         >  1:739444/1‑237 (MQ=255)
                                                                                                   aCATGCAGATCTACAGCGCCAGAGTGCTGACGCCTTACTGGCAATGCTTCCACAGCGTAAATACACCCACGTACTGGACGCGGGTTGTGGACCTGGCTGGATGAGCCGCCACTGGCGGGAACGTCACGCGCAGGTGACGGCCTTAGATCTCTCGCCGCCAATGCTTGTTCAGGCACGCCCGAAAGATGCCGCAGACCCTTTTCCTGCGGGGGATATCGGATCCCTCCCGTTAGCGACTg                                                                                                                                        >  1:328008/1‑239 (MQ=255)
                                                                                                               aCAGCCCCGGAGTTCTGACCCCTTACTGACAAGGCTCCCACAGCGTAAATACCCCCACGTACTGGCCGCGGGTTGTGGACCTGGCGGGATGAGCCGCCACTGGCGGGAACGTCACGCGCAGGTGACGGCCTTAGATCTCTCGCCGCCAATGCTTGTTCAGGCACGCCAGAAGGATGCCGCAGACCATTATCTGGCGGGAGATATCGAATCCCTGCCGTTAGCGACTGCGACGTTCGATc                                                                                                                           <  1:600848/239‑1 (MQ=255)
                                                                                                                     ccAGAGTGCTGACGCCTTACTGGCAATGCTTCCACAGCGTAAATACACCCACGTACTGGACGCGGGTTGTGGACCTGGCTGGATGAGCCGCCACTGGCGGGAACGTCACGCGCAGGTGACGGCCTTAGATCTCTCGCCGCCAATGCTTGTTTCGGCACGCCAGAAGGATGCCGCAGACCATTATCTGGCGGGAGATATCGAATCCCTCCCGTTAGCGGCTGCGACGGTCGCTCttt                                                                                                                        >  1:977581/1‑235 (MQ=255)
                                                                                                                                aCGCCTTACTGGCAATGCTTCCACAGCGTAAATACACCCACGTACTGGACGCGGGTTGTGGACCTGGCTGGATGAGCCGCCACTGGCGGGAACGTCACGCGCAGGGGACGGCCTTAGATCTCTCGCCGCCAATGCTTGGTCAGGCACCCCCGAAGGATGCCGCAGACCATTATCTTGCGGGGGATATCGAATCCCTGCCGGTTGGGGCTGCGACGTTTGATCTTGCCTGGGGCaaatt                                                                                                           >  1:755786/1‑235 (MQ=255)
                                                                                                                                                             tAAATACACCCACGTACTGGACGCGGGTTGTGGACCTGGCTGGATGAGCCGCCACTGGCGGGAACGTCACGCGCAGGTGACGGCCTTAGATCTCTCGCCGCCAATGCTTGTTCAGGCACGCCAGAAGGATGCCGCAGACCCTTATCTTGCGGGAGATATCGAATCCCTGCCGCTAGCGACTGCGACGTTCGATCTTTCATGGGGCAAACTCGCAGTGCAGTGGGGCGGGAAATTTTcc                                                                              >  1:337287/1‑238 (MQ=255)
                                                                                                                                                             tAAATACACCCACGTACTGGACGCGGGTTGTGGACCTGGCTGGATGAGCCGCCACTGGCGGGAACGCCACGCGCAGGGGACGGCCTTTGATCTCTCGCCCCCCATGCTTGTTTCGGCCCCCCAGAAGGATGCCCCAGACCCTTTTCTGCCGGGGGCAATCGGACCCCCCCCGTTAGCGCCGGCGCCGCTCCTTCTTGCGTGGGGCAAACTCCCCCGGCAGGGGGGGGGGAATTTAttc                                                                              >  2:746089/1‑236 (MQ=255)
                                                                                                                                                                     cccACGTACTGGACGCGGGTTGTGGACCTGGCTGGATGAGCCGCCACTGGCGGGAACGTCACGCGCAGGTGACGGCCTTAGATCTCTCGCCGCCAATGCTTGGTCAGGCACGCCCGAAGGATGCCGCAGACCCTTATCTGGCGGGGGGTATCGAATCCCTGCCGTTa                                                                                                                                               >  1:61429/1‑167 (MQ=255)
                                                                                                                                                                          gTAGTGGGCGGGGGTTGGGGCCTGGGCTGGATGGCCCGCCACGGGCGGAAACGTCACGCGCAGGTGACGGCCTTAGATCTCTCGCCGCCAATGCTGGTTCAGGCACGCCAGAAGGATGCCGCAGACCATTATCTGGCGGGAGATATCGAATCCCTGCCGTTAGCGACTGCGACGTTCGATCTTGCATGGAGCAATCTCGCAGTGCAGTGGTGCGGTAATTTATCCACGGCACTCCGCGa                                                                <  1:849569/239‑1 (MQ=255)
                                                                                                                                                                           gccggggggggggTTGGGGCCGTGGTGGGATGGGCCGCCCCGGGCGGGAACGTCCGGCGAAGGGGACGGCCTTAGATCTCCCGCCGCAAATGCTTGTTCAGGCCCGCCAGAAGGATGCCGCAGACCATTATCTGGCGGGAGATATCGAATCCCTGCCGTTAGCGACTGCGACGTTCGATCTTGCATGGAGCAATCTCGCAGTGCAGTGGTGCGGTAATTTATCCACGGCACTCCGCGAg                                                               <  1:642578/229‑1 (MQ=255)
                                                                                                                                                                                          gtgGACCTGGCTGGATGAGCCGCCACTGGCGGGAACGTCACGCGGAGGGGACGGCCCTAGATCTCTCGCCGCCAATGCTTTGTCCGGGACGCCACAAGGAATCCGCAGACCATTTTCTGGCGGGGGGAATCGAACCCCCGCCCGTTGGCGCTCCGCACGTCCGTCTTTGCTGGGGCAATATCCCAGGGCAGGGGGGGGGGGAATTTTCCACCGCCCCCCCCGAGCTGGGTCgggggggg                                                >  2:939461/1‑237 (MQ=255)
                                                                                                                                                                                                             ccgccACTGGCGGGAACGTCACGCGCAGGGGACGGCCTTAGATCTCTCGCCGCCCATGCTTGTTCAGGCACGCCCGAAGGATGGCGCAGACCCTTATCTGGCGGGGGATATTCGATCCCCTCCGGTTGCGGCCGCGACGTTCGGTCTTTCCTTGGGCAATCTCCCCGGGCCGGGGGGCGGTGAATTTTCCCACGCACCCCCCGAGCCGTAGCGGGGGGGGGGGCCCaaaagggggggcg                             >  2:261796/1‑229 (MQ=255)
                                                                                                                                                                                                                 cACGGGCGGGAACGTCGGCGGCGGGGCGCGGCCTGAGATCTTCCGCCGCCAATGCTTGTTCAGGCACGCCAGAAGGGGTCCGCAGCCCATTATCGGGCGGGAGATATCGAATCCATGCCGTTAGCGACTGCGACGTTCGATCTTGCATGGAGCAATCTCGCAGTGCAGTGGTGCGGTAATTTATCCACGGCACTCCGCGAGCTGTATCGGGTGGTGCGCCCCAAAGGCGTGGTCGCGtt                         <  2:1072977/239‑1 (MQ=255)
                                                                                                                                                                                                                                         ggTGACGGCCTTAGATCTCTCGCCGCCAATGCTTGTTCAGGCACGCCAGAAGGATGCCGCAGACCATTATCTGGCGGGAGATATCGAATCCCTGCCGTTAGCGACTGCGACGTTCGATCTTGCATGGAGCAATCTCGCAGTGCAGTGGTGCGGTAATTTATCCACGGCACTCCGCGAGCTGTATCGGGGGGGGGGCCCCAAAGGGGTGGTCGCGTTTACCACCCTGGGGCgggggtggt  >  2:577932/1‑234 (MQ=255)
                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                               
ATGCAGGATATCGACCGTCTGCTGGAGGTGCTGCATGGCAACGGTTAATAAACAAGCCATTGCAGCGGCATTTGGTCGGGCAGCCGCACACTATGAGCAACATGCAGATCTACAGCGCCAGAGTGCTGACGCCTTACTGGCAATGCTTCCACAGCGTAAATACACCCACGTACTGGACGCGGGTTGTGGACCTGGCTGGATGAGCCGCCACTGGCGGGAACGTCACGCGCAGGTGACGGCCTTAGATCTCTCGCCGCCAATGCTTGTTCAGGCACGCCAGAAGGATGCCGCAGACCATTATCTGGCGGGAGATATCGAATCCCTGCCGTTAGCGACTGCGACGTTCGATCTTGCATGGAGCAATCTCGCAGTGCAGTGGTGCGGTAATTTATCCACGGCACTCCGCGAGCTGTATCGGGTGGTGCGCCCCAAAGGCGTGGTCGCGTTTACCACGCTGGTGCAGGGATCGT  >  NC_000913/811488‑811957

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: