Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,872,402 T→G 12.3% V121G (GTG→GGG)  yeaJ → putative diguanylate cyclase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,872,4020TG12.3% 310.9 / 4.2 123V121G (GTG→GGG) yeaJputative diguanylate cyclase
Reads supporting (aligned to +/- strand):  ref base T (68/35);  new base G (2/17);  total (71/52)
Fisher's exact test for biased strand distribution p-value = 6.84e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 124 total aligned reads displayed.

TGCAGAAAGCAGACTCCTCCTTTCTTTATGATAAGTATCAGAATCAGAGTATTGCCGCGCATGTGATGCGCGCTCTCGCTGCTGAGCAGTCGGAAGTGTCGCCAGAACAGCGGCGCGCCATCTGCGAGGCTTTTGAGTCTGCCAATAACACCCATGGCTTAAACCTGACTGCCCATAAATACCCGGGCTTACGCGGCACACTACAAACCGCATCCACTGACTGCGACACAATTGTGGAAGCTGCAGCACTATTACCCGCTTTTGATCAGGCAGTGGAAGGCAACCGCCACCAGGATGATTACGGTTCAGGTCTTGGGATGGCCGAAGAGAAATTTCACTATTATCTCGATCTCAATGACCGCTATGTCTATTTTTATGAGCCGGTTAATGTTGAATACTTTGCGATGAATAACTGGTCCTTCCTGCAGTCAGGAAGTATTGGCATCGATCGCAAAGATATTGAAAAGGTATTT  >  NC_000913/1872168‑1872640
                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                
tGCAGAAAGCAGACTCCTCCTTTCTTTATGATAAGTATCAGAATCAGAGTATTGCCGCGCATGTGATGCGCGCTCTCGCTGCTGAGCAGTCGGAAGTGTCGCCAGAACAGCGGCGCGCCATCTTCGCGGCTTTTGAGTCTGCCAATAACACCCATGGCTTAAACCTGACTGCCCATAAATACACGGGCTTACGCGGCACACGACAAACCGCATCCACTGACTGGGACACAATTGTGGaa                                                                                                                                                                                                                                             >  1:868484/1‑239 (MQ=255)
tGCAGAAAGCAGACTCCTCCTTTCTTTATGATAAGTATCAGAATCAGAGTATTGCCGCGCATGTGATGCGCGCTCTCGCTGCTGAGCAGTCGGAAGTGTCGCCAGAACAGCGGCGCGCCATCTGCGAGGCTTTTGAGTCTGCCACTAACACCCATGGCTTAAACCTGCCTGCCCATAAATACCCGGGCTTACGCGGCACACTACAAACCGCATCCACTGACTGCGAACCAAATGTGGaa                                                                                                                                                                                                                                             >  2:872882/1‑239 (MQ=255)
tGCAGAAAGCAGACTCCTCCTTTCTTTATGATAAGTATCAGAATCAGAGTATTGCCGCGCATGTGATGCGCGCTCTCGCTGCTGAGCAGTCGGAAGTGTCGCCAGAACAGCGGCGCGCCATCTGCGAGGCTTTTGAGTCTGCCAATAACACCCATGGCTTAAACCTGACTGCCCATAAATACCCGGGCTTACGCGGCCCACTCAAAACCCCATCCCCTGACTGCGACACAATTGTGGaa                                                                                                                                                                                                                                             >  2:273892/1‑239 (MQ=255)
tGCAGAAAGCAGACTCCTCCTTTCTTTATGATAAGTATCAGAATCAGAGTATTGCCGCGCATGTGATGCGCGCTCTCGCTGCTGAGCAGTCGGAAGTGTCGCCAGAACAGCGGCGCGCCATCTGCGAGGCTTTTGAGTCTGCCAATAACACCCATGGCTTAAACCTGACTGCCCATAAATACCCGGGCTTACGCGGCACACTACAAACCGCATCCACTGACTGCGCCCCAATTGTGGa                                                                                                                                                                                                                                              >  1:1027594/1‑238 (MQ=255)
tGCAGAAAGCAGACTCCTCCTTTCTTTATGATAAGTATCAGAATCAGAGTATTGCCGCGCATGTGATGCGCGCTCTCGCTGCTGAGCAGTCGGAAGTGTCGCCAGAACAGCGGCGCGCCATCTGCGAGGCTTTTGAGTCTGCCAATAACACCCATGGCTTAAACCTGACTGCCCATAAATACCCGGGCTTACGCGGCACACTACAAACCGCATCCACTGACTGCGCCACAATTTTGGaa                                                                                                                                                                                                                                             >  1:117147/1‑239 (MQ=255)
tGCAGAAAGCAGACTCCTCCTTTCTTTATGATAAGTATCAGAATCAGAGTATTGCCGCGCATGTGATGCGCGCTCTCGCTGCTGAGCAGTCGGAAGTGTCGCCAGAACAGCGGCGCGCCATCTGCGAGGCTTTTGAGTCTGCCAATAACACCCATGGCTTAAACCTGACTGCCCATAAATACCCGGGCTTACGCGGCACACTACAAACCGCATCCACTGACTGCGACACAATTGTGGaa                                                                                                                                                                                                                                             >  1:269718/1‑239 (MQ=255)
       agcactcctctcctTTCTTTTTGATAAGTATCAGAATCAGAGTATTGCCGCGCATGTGATGCGCGCTCTCGCTGCTGAGCAGGCGGAAGTGTCGCCAGAACAGCGGCGCGCCATCTGCGAGGCTTTTGAGTCTGCCAATAACACCCATGGCTTAAACCTGACTGCCCATAAATACCCGGGCTTACGCGGCACACTACAAACCGCATCCACTGACTGCGACACAATTGTGGAAGCTgcag                                                                                                                                                                                                                                      <  2:1079016/230‑1 (MQ=255)
       aGCAGACTCCTCCTTTCTTTATGATAAGTATCAGAATCAGAGTATTGCCGCGCATGTGATGCGCGCTCTCGCTGCTGAGCAGTCGGAAGTGTCGCCAGAACAGCGGCGCGCCATCTGCGAGGCTTTTGAGTCTGCCAATAACACCCATGGCTTAAACCTGACTGCCCATAAATACCCGGGCTTACGCGGCACACTACAAACCGCATCCACTGACTGCGACACAATTGTGGAAGCTgcag                                                                                                                                                                                                                                      <  1:754305/239‑1 (MQ=255)
       aGCAGACTCCTCCTGTCATTATGATAAGTATCAGAATCAGAGTATTGCCGCGCAGGTGATGCGCGCTCTCGCTGCTGAGCAGGCGGAAGTGTCGCCAGAACAGCGGCGCGCCATCTGCGAGGCTTTTGAGTCTGCCAATAACACCCATGGCTTAAACCTGACTGCCCATAAATACCCGGGCTTACGCGGCACACTACAAACCGCATCCACTGACTGCGACACAATTGTGGAAGCTgcag                                                                                                                                                                                                                                      <  1:309732/239‑1 (MQ=255)
          aGACTCCTCCTTTCTTTATGATAAGTATCAGAATCAGAGTATTGCCGCGCATGTGATGCGCGCTCTCGCTGCTGAGCAGTCGGAAGTGTCGCCAGAACAGCGGCGCGCCATCTGCGAGGCTTTTGAGTCTGCCAATAACACCCATGGCTTAAACCTGACTGCCCCTAAATACCCGGGCTTACGCGGCACACTACAAACCGCACCCCCTGACTGCGACACAAATGTGGAAGCTgcacaac                                                                                                                                                                                                                                   >  2:84159/1‑235 (MQ=255)
               cctcctTTCTTTATGATAAGTATCAGAATCAGAGTATTGCCGCGCATGTGATGCGCGCTCTCGCTGCTGAGCAGTCGGAAGTGTCGCCAGAACAGCGGCGCGCCATCTGCGAGGCTTTTGAGTCTGCCAATAACACCCATGGCTTAAACCTGACTGCCCATAAATACCCGGGCTTACCCGGCAAACTACAAACCCCATCCACTGACTCCGACCCAATTGTGGAAGCTGAAGCACCAtt                                                                                                                                                                                                                               >  2:625998/1‑238 (MQ=255)
                ctcctTTCATTATGATAAGTATCAGAATCAGAGTATTGCCGCGCATGTGATGCGCGCTCTCGCTGCTGAGCAGTCGGAAGTGTCGCCAGAACAGCGGCGCGCCATCTGCGAGGCTTTTGAGTCTGCCAATACCCCCCATGGCTTCAACCTGACTGCCCATAAATACCCGGGCTTACGCGGCACCCTACAAACCGCATCCCCTGCCTGCCACACACTTGGGGCAGCTGCACCACTAttc                                                                                                                                                                                                                              >  2:833884/1‑237 (MQ=255)
                   ctttctttATGATAAGTATCAGAATAAGAGTATTGCCGCGCATGTGATGCGCGCTCTCGCTGCTGAGCAGTCGGAAGTGTCGCCAGAACAGCGGCGCGCCATCTGCGAGGCTTTTGAGTCTGCCAATAACACCCATGGCTTAAACCTGACTGCCCATAAATACCCGGGCTTACGCGGCACACTACAAACAGCTTCCACTCACTGCGACACCATTGTGGAAGCTGCAGCACTATTaac                                                                                                                                                                                                                            >  1:362760/1‑235 (MQ=255)
                        tttATGATAAGTATCAGAATCAGAGTATTGCCGCGCATGTGATGCGCGCTCTCGCTGCTGAGCAGTCGGAAGTGTCGCCAGAACAGCGGCGCGCCATCTGCGAGGCTTTTGAGTCTGCCAATAACACCCATGGCTTAAACCTGACTGCCCATAAATACCCGGGCTTACGCGGCACACTACAAACCGCATCCACTGACTGCGACACAATTGTGGAAGCTCCAGCCCTATTACCCGCtttt                                                                                                                                                                                                                     >  1:42209/1‑239 (MQ=255)
                          tATGATAAGTATCAGAATCAGAGTATTGCCGCGCATGTGATGCGCGCTCTCGCTGCTGAGCAGTCGGAAGTGTCGCCAGAACAGCGGCGCGCCATCTGCGAGGCTTTTGAGTCTGCCAATAACACCCATGGCTTAAACCTGACTGCCCATAAATACCCGGGCTTACGCGGCACACTACAAACCGCATCCACTGACTGCGCCACAATTTTGGAAGCTGAAGCCCCATTACCCGCttttcg                                                                                                                                                                                                                   >  1:204477/1‑237 (MQ=255)
                          tATGATAAGTATCAGAATCAGAGTATTGCCGCGCATGTGATGCGCGCTCTCGCTGCTGAGCAGTCGGAAGTGTCGCCAGAACAGCGGCGCGCCATCTGCGAGGCTTTTGAGTCTGCCAATAACACCCATGGCTTAAACCTGACTGCCCATAAATACCCGGGCTTACGCGGCACACTACAAACCGCATCCACTGACTGCGACACAAATCTGGAAGCTGAAGAACAATAACCCCCTTTTg                                                                                                                                                                                                                    >  2:521245/1‑238 (MQ=255)
                           ttGATAAGTATCAAAATCAGAGTATTGCCGCGCATGTGCTGCGGGCTCTCGCTGCGGAGCAGTCCGAAGTGTCGCCAGAACAGCGGCGCGCCATCTGCGAGGCTTTTGAGTCTGCCAATAACCCCCATGGCTTAAACCTGACTGCCCATAAATACCCGGGCTTACGCGGCACACTACAAACCGCATCCACTGACTGCGACACAATTGTGGAAGCTGCAGCACTATTACCCGCTTTTGAt                                                                                                                                                                                                                  <  2:154461/238‑1 (MQ=255)
                             gATAAGTATCAGAATCAGAGTATTGCCGCGCATGTGATGCGCGCTCTCGCTGCTGAGCAGTCGGAAGTGTCGCCAGAACAGCGGCGCGCCATCTGCGAGGCTTTTGAGTCTGCCAATAACACCCATGGCTTAAACCTGACTGCCCATAAATACCCGGGCTTACGCGGCACACTACAAACCGCATCCACTGACTGCAACCCACTTCTGGAAGCTGCAGCACAATTACCCCCTTTTGAtaa                                                                                                                                                                                                                >  2:184456/1‑237 (MQ=255)
                                aattttcaaaatcaggGTATTGGCGCGCAGGTGATGCGCGCCTTCGCTGCTGAGGAGTCGGAAGTGTCGCCAGAACAGCGGCGCGCCATCTGCGAGGCTTTTGAGTCTGCCAATAACACCCATGGCTTAAACCTGACTGCCCATAAATACCCGGGCTTACGCGGCACACTACAAACCGCATCCACTGACTGCGACACAATTGTGGAAGCTGCAGCACTATTACCCGCTTTTGATCAgg                                                                                                                                                                                                              <  2:577763/233‑1 (MQ=255)
                                             agagTATTGCCGCGCATGTGATGCGCGCTCTCGCTGCTGAGCAGTCGGAAGTGTCGCCAGAACAGCGGCGCGCCATCTGCGAGGCTTTTGAGTCTGCCAATAACACCCATGGCTTAAACCTGACTGCCCATAAATACCCGGGCTTACGCGGCACACTACAAACCGCATCCACTGACTGCGACACAATTGTGGAAGCTGCAGCACTATTACCCGCTTTTGATCAGGCAGTGGAAGGCaa                                                                                                                                                                                                >  1:33584/1‑238 (MQ=255)
                                                      ccGCGCATGTGATGCGCGCTCTCGCTGCTGAGCAGTCGGAAGTGTCGCCAGAACAGCGGCGCGCCATCTGCGAGGCTTTTGAGTCTGCCAATAACACCCATGGCTTAAACCTGACTGCCCATAAATACCCGGGCTTACGCGGCACACTACAAACCGCATCCACTGACTGCGACACAATTGTGGAAGCTGCAGCACTATTACCCGCTTTTGATCAGGCAGTGGAAGGCAACCGCCACCAg                                                                                                                                                                                      >  1:808312/1‑239 (MQ=255)
                                                         cgcATGTGATGCGCGCTCTCGCTGCTGAGCAGTCGGAAGTGTCGCCAGAACAGCGGCGCGCCATCTGCGAGGCTTTTGAGTCTTCCAATAACACCCATGGCTTAAACCTGACTGCCCATAAATACCCGGGCTTACGCGGCACACTACAAACCGCATCCACTGACTGCGACACAATTTTGGAAGCTGCAGCACTATTACCCCCCTTTGATCAGGCACGGGAAGGCAACCGCCACCAgggg                                                                                                                                                                                   >  2:319132/1‑237 (MQ=255)
                                                           cATGTGATGCGCGCTCTCGCTGCTGAGCAGTCGGAAGTGTCGCCAGAACAGCGGCGCGCCATCTGCGAGGCTTTTGAGTCTGCCAATAACACCCATGGCTTAAACCTGACTGCCCATAAATACCCGGGCTTACGCGGCACACTCCAAACACCATCCACTGACTGCGACCCAATTACGGACGCACCAGCACTATTACCCCCTTTTGATCCGCAATGGAAAGACAACCCCCACCAgacagc                                                                                                                                                                                 >  2:331800/1‑234 (MQ=255)
                                                              gtgATGCGCGCTCCCGGTGTGGAGCAGTCGGAATGGCCGCAAGAACAGGGGCGCGCCATTTGCGAGGCTTTGGAGTCGGCCAATAACACCCATGGCTTAACCCTGACTGCCCATAAATACCCGGGCTTACGCGGCACACTACAAACCGCATCCACTGACTGCGACACAATTGTGGAAGCTGCAGCACTATTACCCGCTTTTGATCAGGCAGTGGAAGGCAACCGCCACCAGGATGATTa                                                                                                                                                                              <  1:1013819/239‑1 (MQ=255)
                                                              gggATGCGCGCTCTCGCTGCTGAGCAGTCGGAAGTGTCGCCAGAACAGCGGCGCGCCATCTGGAAGGCTTTTGAGTCTTCCAATAACACCCATGGCTTAAACCTGACTGCCCATAAATCCCCGGGCTTACGCGGCACACTACAAACCGCATCCACTGACTGCGACACAATTGTGGAAGCTGCAGCACTATTACCCGCTTTTGATCAGGCAGTGGAAGGCAACCGCCACCAGGATGATTa                                                                                                                                                                              <  1:319132/237‑1 (MQ=255)
                                                                   gcgcctcctcGCTGCTGAGCAGTCGGAAGTGTCGCCAGAACAGCGGCGCGCCATCTGCGAGGCTTTTGAGTCTGCCAATAACACCCATGGCTTAAACCTGACTGCCCATAAATCCCCGGGCTTACGCGGCACACTACAAACCGCATCCACTGACTGCGACACAATTGTGGAAGCTGCAGCACTATTACCCGCTTTTGATCAGGCAGTGGAAGGCAACCGCCACCAGGATGATTACGGtt                                                                                                                                                                         <  1:84159/232‑1 (MQ=255)
                                                                     gcccccccgcgGCTGGGAAGTGGGAAGTGTCCCCAAAACGGCGGCGCCCCAGCTGCGAGGTTTTTGGGCCCGCCAATAACCCCCATGGCTTAACCCTGACTGCCCATAAATCCCCGGGCTTACGCGGCACACTACAAACCGCATCCACTGACTGCGACACAATTGTGGAAGCTGCAGCACTATTCCCCGCTTTTGATCAGGCAGTGGAAGGCAACCGCCACCAGGATGATTACGGTTc                                                                                                                                                                        <  2:543802/231‑1 (MQ=255)
                                                                        ctctCGCTGCTGAGCAGTCGGAAGTGTCGCCAGAACAGCGGCGCGCCATCTGCGAGGCTTTTGAGTCTGCCAATAACACCCATGGCTTAAACCTGACTGCCCATAAATACCCGGGCTTACGCGGCACACTACAAACCGCATCCACTGACTGCGACACAATTGTGGAAGCTGCAGCACTATTACCCGCTTTTGATCAGGCAGTGGAAGGCAACCGCCACCAGGATGATTACGGTTCAgg                                                                                                                                                                     >  1:997332/1‑238 (MQ=255)
                                                                             cgtgctgGGCAGTCGGAAGGGTCGCCAGAACAGCGGCGCCCCATCTGCGAGGCTTTTGAGTCTGCCAATAACACCCATGGCTTAACCCTGACTGCCCATAAATACCCGGGCTTACGCGGCACACTACAAACCGCATCCACTGACTGCGACACAATTGTGGAAGCTGCAGCACTATTACCCGCTTTTGATCAGGCAGTGGAAGGCAACCGCCACCAGGATGATTACGGTTCAGGTCTTgg                                                                                                                                                               <  1:284899/237‑1 (MQ=255)
                                                                                     gCAGTCGGAAGTGTCGCCAGAACAGCGGCGCGCCATCTGCGAGGCTTTTGAGTCTGCCAATAACACCCATGGCTTAAACCTGCCTGCCCATAAATCCCCGGGCTTACGCGGCACACTACAAACCGCATCCACTGACTGCGACACAATTGTGGAAGCTGCAGCACTATTACCCGCTTTTGATCAGGCAGTGGAAGGCAACCGCCACCAGGATGATTACGGTTCAGGTCTTGGGATGGcc                                                                                                                                                        <  1:184456/238‑1 (MQ=255)
                                                                                         tCGGAAGTGTCGCCAGAACAGCGGCGCGCCATCTGCGAGGCTTTTGAGTCTGCCAATAACACCCATGGCTTAAACCTGACTGCCCATAAATACCCGGGCTTACGCGGCACACTACAAACCGCATCCACTGACTGCGCCACAATTGTGGAAGCTGAAGCACTCTTACCCGCTTTTGCTCAGGCAGGGGAAGGAAACCGCCCACAAGATGATTCAGGTTTAGGTCTTTGGAAGGACGaaaa                                                                                                                                                   >  2:369860/1‑237 (MQ=255)
                                                                                         tCGGAAGTGTCGCCAGAACAGCGGCGCGCCATCTGCGAGGCTTTTGAGTCTGCCAATAACACCCATGGCTTAAACCTGACTGCCCATAAATACCCGGGCTTACGCGGCACACTACAAACCGCATCCACTGACTGCGACACAATTGTGGAAGCTGCAGCACTATTACCCGCTTTTGATCAGGCAGTGGAAGGCAACCGCCCCCAGGATGCTTAAGGTTCCGGGCTTGGGATGTCCGAaga                                                                                                                                                   >  1:966920/1‑239 (MQ=255)
                                                                                         tCGGAAGTGTCGCCAGAACAGCGGCGCGCCATCTGCGAGGCTTTTGAGTCTGCCAATAACACCCATGGCTTAAACCTGACTGCCCATAAATACCCGGGCTTACGCGGCACACTACAAACCGCATCCACTGACTGCGACACAATTGTGGAAGCTGCAGCACTATTACCCGCTTTTGATCAGGCAGTGGAAGGCAACCGCCACCAGGATGATTACGGTTCAGGGCTTGGGATGGCCGAaga                                                                                                                                                   >  1:558251/1‑239 (MQ=255)
                                                                                         tCGGAAGTGTCGCCAGAACAGCGGCGCGCCATCTGCGAGGCTTTTGAGTCTGCCAATAACACCCATGGCTTAAACCTGACTGCCCATAAATACCCGGGCTTACGCGGCACACTACAAACCGCATCCACTGACTGCGACACAATTGTGGAAGCTGCAGCACTATTACCCGCTTTTGATCAGGCAGTGGAAGGCAACCGCCACCAGGATGATTACGGTTCAGGCCTTGGGATGGCCGAaga                                                                                                                                                   >  1:346207/1‑239 (MQ=255)
                                                                                         tCGGAAGTGTCGCCAGAACAGCGGCGCGCCATCTGCGAGGCTTTTGAGTCTGCCAATAACACCCATGGCTTAAACCTGACTGCCCATAAATACCCGGGCTTACGCGGCACACTACAAACCGCATCCACTGACTGCGACACAATTGTGGAAGCTGCAGCACTATTACCCGCTTTTGACCAGGCAGTGGAAGGCAACCGCAACCAGGATGATTACGGTTCAGGTCTTGGGATGGCCGacgg                                                                                                                                                   >  1:922710/1‑236 (MQ=255)
                                                                                                 gtCCCCAAAACAGCGGCGCCCCATCGGCGAGGCTTTGGAGTCTGCCAATAACCCCCATGGCTTAAACCGGACGGCCCAAAAATCCCCGGGCTTCCGCGGCACACACCAAACCGCATCCACTGACTGCGACACAATTGGGGAAGCTGCAGCACTATTACCCGCTTTTGATCAGGCAGTGGAAGGCAACCGCCACCAGGATGATTACGGTTCAGGTCTTGGGATGGCCGAAGAGAAAttt                                                                                                                                            <  2:1066289/238‑1 (MQ=255)
                                                                                                   cGCCAGAACAGCGGCGCGCCATCTGCGAGGCTTTTGAGTCTGCCAATAACACCCATGGCTTAAACCTGACTGCCCATAAATACCCGGGCTTACGCGGCACACTACAAACCGCATCCACTGACTGCGACACAATTGTGGAAGCTGCAGCACTATTACCCGCTTTTGATCAGGCAGTGGAAGGCAACCGCCACCAGGATGATTACGGTTCAGGTCTTGGGATGGCCGCAGAGAAATTTCAc                                                                                                                                         >  1:23746/1‑239 (MQ=255)
                                                                                                   cGCCAGAACAGCGGCGCGCCATCTGCGAGGCTTTTGAGTCTGCCAATAACACCCATGGCTTAAACCTGACTGCCCATAAATACCCGGGCTTACGCGGCACACTACAAACCGCATCCACTGACTGCGACACAATTGTGGAAGCTGCAGCACTATTACCCGCTTTTGATCAGGCAGTGGAAGGCAACCGCCACCAGGATGATTACGGTTCAGGTCTTGGGATGGCCGAAGAGAAATTTc                                                                                                                                           >  1:771354/1‑237 (MQ=255)
                                                                                                       aGAACAGCGGCGCGCCATCTGCGAGGCTTTTGAGTCTGCCAATAACACCCATGGCTTAAACCTGACTGCCCATAAATACCCGGGCTTACGCGGCACACTACAAACCGCATCCACTGACTGCGACACAATTGTGGAAGCTGCAGCACTATTACCCGCTTTTGATCAGGCAGTGGAAGGCAACCGCCACCAGGATGGTTACGGTTCAGGTCTTGGGATGGCCGAAGAGAAATTTCACtatt                                                                                                                                     >  2:941922/1‑239 (MQ=255)
                                                                                                              cggcgCGCCATCTGCGAGGCTTTTGAGTCTGCCAATAACACCCATGGCTTAAACCTGACTGCCCATAAATACCCGGGCTTACGCGGCACACTACAAACCGCATCCACTGACTGCGACACCATTGTGGAAGCTGCAGCACTATTACCCGCTTTTGATCAGGCAATGGCAGGCAACCGCCACCAGGATGATTACGGTTCAGGTCTTGGGATGGCCGGAGAGACATTTCACTATTATCTCGa                                                                                                                              >  1:1077836/1‑239 (MQ=255)
                                                                                                                   cgcCATCTGCGAGGCTTTTGAGTCTGCCAATAACACCCATGGCTTAAACCTGACTGCCCATAAATACCCGGGCTTACGCGGCACACTACAAACCGCATCCACTGACTGCGCCACAATTGTGGAAGCTGCAGCACTATTACCCGCTTTTGATCAGGCAGTGGCAGGCAACCGCCACCAGGATGATTACGGTTACGGTCTTGGGATGGCCGACAAGAAATTTCCCTATTTTCTCGAtctcc                                                                                                                         >  1:25189/1‑238 (MQ=255)
                                                                                                                                  ttttGAGTCTGCCAATAACACCCATGGCTTAAACCTGACTGCCCATAAATACCCGGGCTTACGCGGCACACTACAAACCGCATCCACTGACTGCGACACAATTGTGGAAGCTGCAGCACTATTACCCGCTTTTGATCAGGCAGTGGAAGGCAACCGCCACCAGGATGATAACGGTTCAGGCCTTGGGATGGCCGAGTAGAAATTTCACTATTATCTCGTTCTCACTGACCGCTAGGTCt                                                                                                          >  1:610529/1‑239 (MQ=255)
                                                                                                                                    ttGAGTCTGCCAATAACACCCATGGCTTAAACCTGACTGCCCATAAATACCCGGGCTTACGCGGCACACTACAAACCGCATCCACTGACTGCGACACAATTGTGGAAGCTGCAGCACTATTACCCGCTTTTGATCAGGCAGTGGAAGGCAACCGCCACCAGGATGATTACGGTTCAGGTCTTGGGATGGCCGAAGAGAAATTTCCCTATTATCTCGATCTCAAAGACCCCTATGTCTAt                                                                                                        >  2:130514/1‑239 (MQ=255)
                                                                                                                                    ttGAGTCTGCCAATAACACCCATGGCTTAAACCTGACTGCCCATAAATACCCGGGCTTACGCGGCACACTACAAACCGCATCCACTGACTGCGACACAATTGTGGAAGCTGCAGCACTATTACCCGCTTTTGATCAGGCAGTGGAAGGCAACCGCCACCAGGATGATTACGGTTCAGGTCTTGGGATGGCCCAAAAGAAATTTCCCTCTTACTTATATCTAAAATCCCGCTATGTCCAt                                                                                                        >  2:119188/1‑239 (MQ=255)
                                                                                                                                           tGCCAATAACACCCATGGCTTAAACCTGACTGCCCATAAATACCCGGGCTTACGCGGCACACTACAAACCGCATCCACTGACTGCGACACAATTGTGGAAGCTGCAGCACTATTACCCGCTTTTGATCAGGCAGTGGAAGGCAACCGCCACCAGGATGATTACGGTTCAGGCCTTGGGATGGCCGACGGGAAATTTCACTATTATCTCGATCTCAATGACCGCTATGTCTAATTTTAta                                                                                                 >  1:475399/1‑238 (MQ=255)
                                                                                                                                            gCCAATAACACCCATGGCTTAAACCTGACTGCCCATAAATACCCGGGCTTACGCGGCACACTACAAACCGCATCCACTGACTGCGACACAATTGTGGAAGCTGCAGCACTATTACCCGCTTTTGATCAGGCAGTGGAAGGCAACCGCCACCAGGTTGATTACGTTTCAGGGCTTGGGATGGCCGAAGAGAAATTCCAATATTATCTCGACCTCAAGGAACGCAATGTTAATTTTAATGa                                                                                                >  2:100252/1‑239 (MQ=255)
                                                                                                                                            gCCAATAACACCCATGGCTTAAACCTGACTGCCCATAAATACCCGGGCTTACGCGGCACACTACAAACCGCATCCACTGACTGCGACACAATTGTGGAAGCTGCAGCACTATTACCCGCTTTTGATCAGGCAGTGGAAGGCAACCGCCACCAGGATGATTACGGTTCAGGTCTTGGGATGGCCGAAGAGAAATTTCACTATTATATCGATCTCAATGACCGCTATGTCTAtttttttta                                                                                                >  1:997361/1‑235 (MQ=255)
                                                                                                                                              cAATACCACCAAGGGCTTAAACCTGACTTCCCAAAAATCCCCGGGCTTACGCGGCCCACTACAAACCGCATCCACTGACTGCGACACAATTGTGGAAGCTGCAGCACTATTACCCGCTTTTGATCAGGCAGTGGAAGGCAACCGCCACCAGGATGATTACGGTTCAGGTCTTGGGATGGCCGAAGAGAAATTTCACTATTATCTCGATCTCAATGACCGCTATGTCTATTTTTATGAg                                                                                               <  1:872882/238‑1 (MQ=255)
                                                                                                                                                  accccccATGGCTTAACCCGTACCGCCAATAAATCCCCGGGCTTACGCGGACCACAAAAACCCCCATCCACTGACTGCGACACAATTGGGGAAGCTGCAGCACTATTCCCCGCTTTTGATCAGGCAGTGGAAGGCAACCGCCACCAGGATGATTACGGTTCAGGTCTTGGGATGGCCGAAGAGAAATTTCACTATTATCTCGATCTCAATGACCGCTATGTCTATTTTTATGAGCCGGt                                                                                          <  2:200541/235‑1 (MQ=255)
                                                                                                                                                    ccccctgggtttAACCCGGCCTGCCAATAAAACCCCGGGCTTCCGCGGCACCCTACAACCCCCATCCACTGACGGCGACACAATTGGGGAAGCTGCAGCACTATTACCCGCTTTTGATCAGGCAGTGGAAGGCAACCGCCACCAGGATGATTACGGTTCAGGTCTTGGGATGGCCGAAGAGAAATTTCACTATTATCTCGATCTCAATGACCGCTATGTCTATTTTTATGAGCCGGTTa                                                                                        <  2:305422/229‑1 (MQ=255)
                                                                                                                                                    cccccGTGGTTTAAACCTGCCTGCCCATAAACACCCGGCTTTAGCGGACACAGTACAAACCGCGTCCACTGACGGCGACACAATTGTGGGAGCTGCAGAACTAGTCCCCGCTGTTGACCGGGCAGTGGAAGGCAACCGCCACCAGGATGATTACGGTTCAGGTCTTGGGATGGCCGAAGAGAAATTTCACTATTATCTCGATCTCAATGACCGCTATGTCTATTTTTATGAGCCGGTTa                                                                                        <  2:204477/237‑1 (MQ=255)
                                                                                                                                                    cccccATGGCTTAACCCGGACTCCCCATAAATCCCCGGGTTTACGCGGCCCACAACAAACCGCATCCCCTGACGGCGACACAATTGGGGAAGCTGCAGCACTATTACCCGCTTTTGATCAGGCAGGGGAAGGCAACCGCCACCAGGATGATTACGGTTCAGGTCTTGGGATGGCCGAAGAGAAATTTCACTATTATCTCGATCTCAATGACCGCTATGTCTATTTTTATGAGCCGGTTa                                                                                        <  2:42209/237‑1 (MQ=255)
                                                                                                                                                    cacCCATGGCTTAAACCTGACTGCCAATAAATCCCCGGGCTGACGCGGCACCATACAAACCGCCTCCCCTGACTGCGACACAATTGTGGAAGCTGCAGCCCTATTACCCGCTTTTGATCAGGCAGTGGAAGGCAACCGCCACCAGGATGATTACGGTTCAGGTCTTGGGATGGCCGAAGAGAAATTTCACTATTATCTCGATCTCAATGACCGCTATGTCTATTTTTATGAGCCGGTTa                                                                                        <  1:545119/239‑1 (MQ=255)
                                                                                                                                                       ccATGGCTTAAACCTGACTGCCCATAAATACCCGGGCTTACGCGGCACACTACAAACCGCATCCACTGACTGCGACACAATTGTGGCAGCTGCAGCACTATTACCCGCTTTTGATCAGGCAGTCGAAGGCAACCGCCACCAGGATGATTCCGGTTCAGGTCTTGGGATGGCCGAAGAGGAATTTCACTATTATCTCGATCTCAATGACCGCTATGTCTATTTTTCTGAGCCGGTTAATg                                                                                     >  1:782405/1‑239 (MQ=255)
                                                                                                                                                         aTGGCTTAAACCTGACTGCCCATAAATACCCGGGCTTACGCGGCACACTACAAACCGCATCCACTGACTGCGACACAATTGTGGAAGCTGCAGCACTATTACCCGCTTTTGATCAGGCAGTGGAAGGCAACCGCCACCAGGATGATTACGGTTCAGGGCTTGGGATGGCCGAAGAGAAATTTCACTATTATCTCGATCTCAATGACCGCTATGTCTATTTTTATTGGCCGGTTAATGtg                                                                                   >  2:758016/1‑238 (MQ=255)
                                                                                                                                                           ggCTTAACCCTGACGGCCCATAAATACCCGGGCTTACGCGGCACACTACAAACCGCATCCACTGACTGCGACACAATTGGGGAAGCTGAAGCACTATTACCCGCTTTTGATCAGGCAGTGGAAGGCAACCGCCACCAGGATGATTATGGTTCAGGTCTTGGGATGGCCGAAGAGAAATTTCACTATTATCTCGATCTCAATGACCGCTATGTCTATTTTTATGAGCCGGTTAATGTTGa                                                                                 <  1:942602/239‑1 (MQ=255)
                                                                                                                                                                aacccTGACTCCCCTTAATTCCCCGGGTTTACGCGGCACACTACAAACCGCATCCACTGACCGCGACAGAATTGTGGAAGCTGCAGCACTATTACCGGTTTTTGATCAGGCAGTGGAAGGCAACCGCCACCAGGATGATTACGGTTCAGGTCTTGGGATGGCCGAAGAGAAATTTCACTATTATCTCGATCTCAATGACCGCTATGTCTATTTTTATGAGCCGGTTAATGTTGAATACt                                                                            <  2:346207/236‑1 (MQ=255)
                                                                                                                                                                 aaCCTGACTGCCCATAAATACCCGGGCTTACGCGGCACACTACAAACCGCATCCACTGACTGCGACACAATTGTGGAAGCTGCAGCACTATTACCCGCTTTTGATCAGGCAGTGGAAGGCAACCGCCACCAGGATGATTACGGTTCAGGTCTTGGGATGGCCGAAGAGAAAttt                                                                                                                                            >  1:306236/1‑174 (MQ=255)
                                                                                                                                                                 aaCCTGACTGCCCATAAATACCCGGGCTTACGCGGCACACTACAAACCGCATCCACTGACTGCGACACAATTGTGGAAGCTGCAGCACTATTACCCGCTTTTGATCAGGCAGTGGAAGGCAACCGCCACCAGGATGATTACGGTTCAGGTCTTGGGATGGCCGAAGAGAAAttt                                                                                                                                            <  2:306236/174‑1 (MQ=255)
                                                                                                                                                                 aaCCTGACTGCCCATAAATACCCGGGCTTACGCGGCACACTACAAACCGCATCCACTGACTGCGACACAATTGTGGAAGCTGCAGCACTATTACCCGCTTTTGATCAGGCAGTGGAAGGCAACCGCCACCAGGATGATTACGGTTCAGGTCTTGGGATGGCCGAAGAGAAATTTCACTATTATCTCGATCTCAATGACCGCTATGTCTATTTTTATGAGCCGGTTAATGTTGAATACtt                                                                           >  1:8796/1‑239 (MQ=255)
                                                                                                                                                                       aCTGCCCATAAATACCCGGGCTTACGCGGCACACTACAAACCGCATCCACTGTCTGCGACACAATTGTGGAAGCTGCAGCACTATTACCCGCTTTTGATCAGGCAGTGGAAGGCAACCGCCACCAGGATGATTACGGTTCAGGTCTTGGGATGGCCGAAGAGAAATTTCCCTATTACCTCGATCTCAATTACCGCTACGTCTATATTTAGGAGCCGGGTAATGTTGAAAACTTTTCggg                                                                     >  2:645205/1‑237 (MQ=255)
                                                                                                                                                                       aCTGCCCATAAATACCCGGGCTTACGCGGCACACTACAAACCGCATCCACTGACTGCGACACAATTGTGGAAGCTGCAGCACTATTAcc                                                                                                                                                                                                                            <  2:657102/89‑1 (MQ=255)
                                                                                                                                                                       aCTGCCCATAAATACCCGGGCTTACGCGGCACACTACAAACCGCATCCACTGACTGCGACACAATTGTGGAAGCTGCAGCACTATTAcc                                                                                                                                                                                                                            >  1:657102/1‑89 (MQ=255)
                                                                                                                                                                       aCTGCCCATAAATACCCGGGCTTACGCGGCACACTACAAACCGCATCCACTGACTGCGACACAATTGTGGAAGCTGCAGCACTATTACCCGCTTTTGATCAGGCAGTGGAAGGCAACCGCCACCAGGATGATTACGGTTCAGGTCTTGGGATGGCCGAAGAGAAATTTCACTATTATCTCGATCTCAATGACCGCTATGTCTATTTTTATGAGCCGGTTAATGTTGAATACTTTGCGAt                                                                     >  1:802331/1‑239 (MQ=255)
                                                                                                                                                                       aCTGCCCATAAATACCCGGGCTTACGCGGCACACTACAAACCGCATCCACTGACTGCGACACAATTGTGGAAGCTGCAGCACTATTACCCGCTTTTGATCAGGCAGTGGAAGGCAACCGCCACCAGGATGATTACGGTTCAGGTCTTGGGATGGCCGAAGAGAAATTTCACTATTATCTCGATCTCAATGACCGCTATGTCTATTTTTATGAGCCGGTTAATGTTGAATACTTTGCGAt                                                                     >  1:565474/1‑239 (MQ=255)
                                                                                                                                                                        cTGCCCATAAATCCCCGGGCTTACGCGGCACACTACAAACCGCATCCACTGACTGCGACACAATTGTGGAAGCTGCAGCACTATTACCCGCTTTTGATCAGGCAGGGGAAGGCAACCGCCACCAGGATGATTACGGTTCAGGTCTTGGGATGGCCGAAGAGAAATTTCACTATTATCTCGATCTCAATGACCGCTATGTCTATTTTTATGAGCCGGTTAATGTTGAATACTTTGCGATg                                                                    <  1:97075/239‑1 (MQ=255)
                                                                                                                                                                             cATAAATACCCGGGCTTACGCGGCACACTACAAACCGCATCCACTGACTGCGACACAATTGTGGAAGCTGCAGCACTATTACCCGCTTTTGATCAGGCAGTGGAAGGCAACCGCCACCAGGATGATTACGGTTAAGGTCTTGGGATGGCCGAAGAGAAATTTCCCTATTATCTCGATCTCAATGACCGCCATGTCTATTTTTATGAGCCGGTTAATTTTGAATACTTTggtcggaaaaa                                                               >  2:869378/1‑229 (MQ=255)
                                                                                                                                                                                aaaTGCCCGGGCTTACGGGGCACACTACAAACCGCTCCCACTGACTGCGACACAATTGGGGAAGCTGCAGCACTATTACCCGCTTTTGATCAGGCAGGGGAAGGCAACCGCCACCAGGATGATTACGGTTCAGGTCTTGGGATGGCCGAAGAGAAATTTCACTATTATCTCGATCTCAATGACCGCTATGTCTATTTTTATGAGCCGGTTAATGTTGAATACTTTGCGATGAATAACTg                                                            <  1:830738/239‑1 (MQ=255)
                                                                                                                                                                                  accccggggCTTACCGGGCACACTACAACCCGCCCCCCCTGCCTGCGACACATTGGTGGAAGCGGCAGCATTATGACCCGTTTTTGACAGGGCAGTGGAGGGCAACCGCCACCAGGATGATTACGGTTCAGGTCTTGGGATGGCCGAAGAGAAATTTCACTATTATCTCGATCTCAATGACCGCTATGTCTATTTTTATGAGCCGGTTAATGTTGAATACTTTGCGATGAATAACTGGt                                                          <  2:808312/233‑1 (MQ=255)
                                                                                                                                                                                     cccGGGCTTACGCGGCACACTACAAACCGCATCCACTGACTGCGACACAATTGTGGAAGCTGCAGCACTATTACCCGCTTTTGATCAGGCAGTGGAAGGCAACCGCCACCAGGATGATTACGGTTCAGGTCTTGGGATGGCCGAAGAGAAATTTCACTATTATCTCGATCTCAATTACCGCTATGTCTATTTTTATTAGCCGGTTAATGTTTAATACTTTTCGATGAAAAAAGGGtcct                                                       >  2:421887/1‑239 (MQ=255)
                                                                                                                                                                                     cccGGGCTTACGCGGCACACTACAAACCGCATCCACTGACTGCGACACAATTGTGGAAGCTGCAGCACTATTACCCGCTTTTGATCAGGCAGTGGAAGGCAACCGCCACCAGGATGATTACGGTTCAGGTCTTGGGATGGCCGAAGAGAAATTTCACTATTATCTCGATCTCAATGACCGCTATGTCTATTTTTATTAGCCGGTTAATGTTGAAAACTTTGCGATGAAAAAAGGGtcct                                                       >  2:524124/1‑239 (MQ=255)
                                                                                                                                                                                     cccGGGCTTACGCGGCACACTACAAACCGCATCCACTGACTGCGACACAATTGTGGAAGCTGCAGCACTATTACCCGCTTTTGATCAGGCAGTGGAAGGCAACCGCCACCAGGATGATTACGGTTCAGGTCTTGGGATGGCCGAAGAGAAATTTCACTATTATCTCGATCTCAATGACCGCTATGTCTATTTTTATGAGCCGGTTAATGTTGAATACTTTGCGATGAATAACTGGtcct                                                       >  1:702011/1‑239 (MQ=255)
                                                                                                                                                                                     cccGGGCTTACGCGGCACACTACAAACCGCATCCACTGACTGCGACACAATTGTGGAAGCTGCAGCACTATTACCCGCTTTTGATCAGGCAGTGGAAGGCAACCGCCACCAGGATGATTACGGTTCAGGTCTTGGGATGGCCGAAGAGAAATTTCACTATTATCTCGATCTCAATGACCGCTATGTCTATTTTTATGAGCCGGTTAATGTTGAATACTTTGCGATGAAAAACGGGtcct                                                       >  2:531785/1‑239 (MQ=255)
                                                                                                                                                                                        gggCTTACGCGGCACACTACAAACCGCATCCACTGACTGCGACACAATTGTGGAAGCTGCAGCACTATTACCCGCTTTTGATCAGGCAGTGGAAGGCAACCGCCACCAGGATGATTACGGTTCAGGTCTTGGGATGGCCGAAGAGAAATTTCACTATTATCTCGATCTCAATGACCGCTATGTCTATTTTTATGAGCCGGTTAATGTTGAATACTTTGCGATGAATAACt                                                             >  1:1074967/1‑230 (MQ=255)
                                                                                                                                                                                         ggCTTACGCGGCACACTACAAACCGCATCCACTGACTGCGACACAATTGTGGAAGCTGCAGCACTATTACCCGCTTTTGATCAGGCAGTGGAAGGCAACCGCCACCAGGATGATTACGGTTCAGGTCTTGGGATGGCCGAAGAGAAATTTCACTATTATCTCGATCTCAATGACCGCTATGTCTATTTTTAt                                                                                                  >  1:698894/1‑192 (MQ=255)
                                                                                                                                                                                          gCTTACGCGGCACACTACAAACCGCATCCACTGACTGCGACACAATTGTGGAAGCTGCAGCACTATTACCCGCTTTTGATCAGGCAGTGGAAGGCAACCGCCACCAGGATGATTACGGTTCAGGTCTTGGGATGGCCGAAGAGAAATTTCACTCTTATCTCGATCTCAATGACCGCTATGTCTATTTTTATGTGCCGGTTAAATTTGAATAATTTGGGATAAATAAATGGtccttcct                                                   >  2:43755/1‑238 (MQ=255)
                                                                                                                                                                                          gCTTACGCGGCACACTACAAACCGCATCCACTGACTGCGACACAATTGTGGAAGCTGCAGCACTATTACCCGCTTTTGATCAGGCAGTGGAAGGCAACCGCCACCAGGATGATTACGGTTCAGGTCTTGGGATGGCCGAAGAGAAATTTCACTATTATCTCGATCTCAATGACCGCTATGTCTATTTTTATGAGCCGGTTAATGTTGAAAACTTTGCGTTGATTAACTGTTCCTTCCTg                                                  >  1:138460/1‑239 (MQ=255)
                                                                                                                                                                                             tACGCGGCACAATACAAACCCCATCCTCTGCCGTGGACACAATTGGGAAACCTGCCGCACTTTTCCCCGTTTTTGACTAGGCAGTGGAAGGCAACCGCCACCAGGATGATTACGGTTCAGGGCTTGGGATGGCCGAAGAGAAATTTCACTATTATCTCGATCTCAATGACCGCTATGTCTATTTTTATGAGCCGGTTAATGTTGAATACTTTGCGATGAATAACTGGTCCTTCCTGCa                                                <  1:170328/238‑1 (MQ=255)
                                                                                                                                                                                              aCGCGGCACACTACAAACCGCATCCACTGACTGCGACACAATTGTGGAAGCTGCAGCACTATTACCCGCTTTTGATCAGGCAGTGGAAGGCAACCGCCACCAGGATGATTACGGTTCAGGTCTTGGGATGGCCGAAGAGAAATTTCACTATTATCTCGATCTCAATGACCGCTATGTCTATTTTTATGAGCCGGTTAATGTTGAATACTTTGCGATGAATAACTGGTCCTTCCTGCAGt                                              >  2:858105/1‑239 (MQ=255)
                                                                                                                                                                                                gcgGCACACTACAAACCGCATCCACTGACTGCGACACAATTGTGGAAGCTGCAGCACTATTACCCGTTTTTGATCAGGCAGTGGAAGGCAACCGCCACCAGGATGATTACGGTTCAGGTCTTGGGATGGCCGAAGAGAAATTTCACTATTATCTCGATCTCAATGACCGCTATGTCTATTTTTATGAGCCGGTTAATGTTGAATACTTTGCGATGAATAACTGGTCCTTCCTGCAGTCa                                            <  1:380057/239‑1 (MQ=255)
                                                                                                                                                                                                     ccctctcacaaCCGCATCCACTGACTGGCACCAAATTGGGGAAGCTGCAGCCCTTTTTCCCGCTTTGGATCAGGCAGTGGAAGGCAACCGCCACCAGGATGATTACGGTTCAGGTCTTGGGATGGCCGAAGAGAAATTTCACTATTATCTCGATCTCAATGACCGCTATGTCTATTTTTATGAGCCGGTTAATGTTGAATACTTTGCGATGAATAACTGGTCCTTCCTGCAGTCAGGa                                         <  1:521245/229‑1 (MQ=255)
                                                                                                                                                                                                     cacaCTACAAACCGCATCCACTGACTGCGACACAATTGTGGAAGCTGCAGCACTATTACCCGCTTTTGATCAGGCAGTGGAAGGCAACCGCCACCAGGATGATTACGGTTCAGGTCTTGGGATGGCCGAAGAGAAATTTCACTATTATCTCGATCTCAATAACCGCTATGTCTATTTTTTTGAGCCGGTTAATGTTGAATACTTTGCGTTGAATAACTGGTCCTTTCTACAGTCAAGaa                                        >  2:947494/1‑239 (MQ=255)
                                                                                                                                                                                                     caaacTACAAACCGCACCCACTGACTGCGACAAAATTGGGGAAGTTGCAGCCATTTTCCCCGTTTTTGATCAGGCAGTGAAAGGCAACCGCCACCAGGATGATTACGGTTCAGGTCTTGGGATGGCCGAAGAGAAATTTCACTATTATCTCGATCTCAATGACCGCTATGTCTATTTTTATGAGCCGGTTAATGTTGAATACTTTGCGATGAATAACTGGTCCTTCCTGCAGTCAGGa                                         <  1:333644/235‑1 (MQ=255)
                                                                                                                                                                                                               aCCCCATCCACTGACTGATGAACAATTGTGGAAGCGGGAGCCCTATTACCCGGTTTTAATCAGGCAGTGGAAGGCAACCGCCCCCAGGATGATTACGGTTCAGGTCTTGGGATGGCCGAAGAGAAATTTCACTATTATCTCGATCTCAATGACCGCTATGTCTATTTTTATGAGCCGGTTAATGTTGAATACTTTGCGATGAATAACTGGTCCTTCCTGCAGTCAGGAAGTATTGGCat                              <  1:947494/239‑1 (MQ=255)
                                                                                                                                                                                                                ccGCACCCACTGACTGACACAAAATGGGGGAAGGCGCAGCACTTTTCCCCTTTTTTGATCAGGCAGGGGAAGGCAACCGCCACCAGGATGATTACGGTTCAGGTCTTGGGATGGCCGAAGAGAAATTTCACTATTATCTCGATCTCAATGACCGCTATGTCTATTTTTATGAGCCGGTTAATGTTGAATACTTTGCGATGAATAACTGGTCCTTCCTGCAGTCAGGAAGTATTGGCatc                             <  2:33584/239‑1 (MQ=255)
                                                                                                                                                                                                                 cccATCCACTGCACGCGCCACAATTGGGGAAAGCGGCGCCATTTTACCCGTTTTTGATCAGGCAGGGGAAGGCAACCGCCACCAGGATGATTACGGTTCAGGTCTTGGGATGGCCGAAGAGAAATTTCACTATTATCTCGATCTCAATGACCGCTATGTCTATTTTTATGAGCCGGTTAATGTTGAATACTTTGCGATGAATAACTGGTCCTTCCTGCAGTCAGGAAGTATTGGCatcg                            <  2:771354/237‑1 (MQ=255)
                                                                                                                                                                                                                    aTCCACTGACTGCGACACAATTGTGGAAGCTGCAGCACTATTACCCGCTTTTGATCAGGCAGTGGAAGGCGACCGCCACCAGGATGATTACGGTTCAGGTCTTGGGATGGCCGAAGAGAAATTTCACTATTATCTCGATCTCAATGACCGCTATGTCTATTTTTATGAGCCGGTTAATGTTGAATACTTTGCGATGAAAAACTGGGCCTTCCTTCAGTCAGGAAGGATTGGCattgaac                         >  2:421099/1‑237 (MQ=255)
                                                                                                                                                                                                                    aTCCACTGACTGCGACACAATTGTGGAAGCTGCAGCACTATTACCCGCTTTTGATCAGGCAGTGGAAGGCAACCGCCACCAGGATGATTACGGTTCAGGt                                                                                                                                                                    >  1:503363/1‑100 (MQ=255)
                                                                                                                                                                                                                    aTCCACTGACTGCGACACAATTGTGGAAGCTGCAGCACTATTACCCGCTTTTGATCAGGCAGTGGAAGGCAACCGCCACCAGGATGATTACGGTTCAGGTCTTGGGATGGCCGAAGAGAAATTTCACTATTATCTCGATCTCAATGACCGCTATGTCTATTTTTATGAGCCGGTTAATGTTGAATACTTTGCGATGAATAACTGGTCCTTCCTGCAGTCAGGAAGTATTGGCatcgat                          >  1:445354/1‑238 (MQ=255)
                                                                                                                                                                                                                    aTCCACTGACTGCGACACAATTGTGGAAGCTGCAGCACTATTACCCGCTTTTGATCAGGCAGTGGAAGGCAACCGCCACCAGGATGATTACGGTTCAGGTCTTGGGATGGCCGAAGAGAAATTTCACTATTATCTCGATCTCAATGACCGCTATGTCTATTTTTATGAGCCGGTTAATGTTGAATACTTTGCGATGAATAACTGGTCCTTCCTGCAGTCAGGAAGTATTGGCa                               >  1:439142/1‑233 (MQ=255)
                                                                                                                                                                                                                    aTCCACTGACTGCGACACAATTGTGGAAGCTGCAGCACTATTACCCGCTTTTGATCAGGCAGTGGAAGGCAACCGCCACCAGGATGATTACGGTTCAGGTCTTGGGATGGCCGAAGAGAAATTTCACTATTATCTCGATCTCAATGACCGCTATGTCTATTTTTATGAGCCGGTTAATGTTGAATACTTTGCGATGAATAACTGTCCTTCCTGCAGTCAGGAAGTATTGGCatcggtcg                        >  1:410098/1‑239 (MQ=255)
                                                                                                                                                                                                                           ggatgcaaaacaATTGTGGAAGCTGCAGCACTATTACCCGTTTTTGATCAGGCAGTGGAAGGCAACCGCCACCAGGATGATTACGGTTCAGGTCTTGGGATGGCCGAAGAGAAATTTCACTATTATCTCGATCTCAATGACCGCTATGTCTATTTTTATGAGCCGGTTAATGTTGAATACTTTGCGATGAATAACTGGTCCTTCCTGCAGTCAGGAAGTATTGGCATCGATCGCAAAGa                  <  1:625998/230‑1 (MQ=255)
                                                                                                                                                                                                                              tgCGACACAATTGTGGAAGCTGCAGCACTATTACCCGCTTTTGATCAGGCAGTGGAAGGCAACCGCCACCAGGATGATTACGGTTCAGGTCTTGGGATGGCCGAAGAGAAATTTCACTATTATCTCGATCTCAATGACCGCTATGTCTCTTTTTATGAGCCGGTTAATGTTGAATACTTTGCGATGAATAACTGGTCCTTCCTGCAGTCAGGAAGTATTGGCATCGATCGCAAAGatat               >  1:789711/1‑239 (MQ=255)
                                                                                                                                                                                                                              tgCGACACAATTGTGGAAGCTGCAGCACTATTACCCGCTTTTGATCAGGCAGTGGAAGGCAACCGCCACCAGGATGATTACGGTTCAGGTCTTGGGATGGCCGAAGAGAAATTTCACTATTATCTCGATCTCAATGACCGCTATGTCTATTTTTATGAGCCGGTTAATGTTGAATACTTTTGGATGAATAAATGGTCCTTCCTGCAGTCAGGAAGAATTGGCATCGATGGAAAAGatat               >  2:759685/1‑239 (MQ=255)
                                                                                                                                                                                                                                cGACACAATTGTGGAAGCTGCAGCACTATTACCCGCTTTTGATCAGGCAGTGGAAGGCAACCGCCACCAGGATGATTACGGTTCAGGCCTTGGGATGGCCGAAGAGAAATTTCACTATTATCTCGATCTCAATGACCGCTATGTCTCTTTTTATGAGCCGGTTAATGTTGAATCCTTTGCGATGACTAACTGGTCCTTCTTGCACTCAGGAAGAATTGGCATCGATTGCAAAGAAATTg             >  2:722970/1‑239 (MQ=255)
                                                                                                                                                                                                                                 gACACAATTGTGGAAGCTGCAGCACTATTACCCGCTTTTGATCAGGCAGTGGAAGGCAACCGCCACCAGGATGATTACGGTTCAGGTCTTGGGATGGCCGAAGAGAAATTTCACTATTATCTCGATCTCAATGACCGCTATGTCTATTTTTATGAGCCGGTTAATg                                                                                     >  1:815110/1‑166 (MQ=255)
                                                                                                                                                                                                                                 gACACAATGGTGGAAGTTCCACCACTTTTCCCCGTTTTTTATAAGGCGGTGGAAGGCAACCGCCACCAGGATGATTACGGTTCAGGTCTTGGGATGGCCGAAGAGAAATTTCACTATTATCTCGATCTCAATGACCGCTATGTCTATTTTTATGAGCCGGTTAATGTTGAATACTTTGCGATGAATAACTGTCCTTCCTGCAGTCAGGAAGTATTGGCATCGATCGCAAAGATATTGaa           <  2:410098/239‑1 (MQ=255)
                                                                                                                                                                                                                                  cccaatttGTGTAAGCTGCCGCCATTTTCCCCGTTTTGGAACGGGCGGGGGAAGGCAACCGCCACCAGGATGATTACGGTTCAGGTCTTGGGATGGCCGAAGAGAAATTTCACTATTATCTCGATCTCAATGACCGCTATGTCTATTTTTATGAGCCGGTTAATGTTGAATACTTTGCGATGAATAACTGGTCCTTCCTGCAGTCAGGAAGTATTGGCATCGATCGCAAAGATATTGaa           <  2:23746/233‑1 (MQ=255)
                                                                                                                                                                                                                                   cacaATTGGGGAAGCTGCAGCACTATTACCCGCTTTTGATCAGGCAGTGGAAGGCAACCGCCACCAGGATGATTACGGTTCAGGTCTTGGGATGGCCGAAGAGAAATTTCACTATTATCTCGATCTCAATGACCGCTATGTCTATTTTTATGAGCCGGTTAATGTTGAATACTTTGCGATGAATAACTGGTCCTTCCTGCAGTCAGGAAGTATTGGCATCGATCGCAAAGATATTGaa           <  1:130514/238‑1 (MQ=255)
                                                                                                                                                                                                                                   accaATTGTGGAAGCTGCACCACTATTACCCGCTTTTGAACAGGCGGGGGAAGGCAACCGCCCCCAGGATGATTACGGTTCAGGTCTTGGGATGGCCGAAGAGAAATTTCACTATTATCTCGATCTCAATGACCGCTATGTCTAGTTTTATGAGCCGGTTAATGTTGAATCCTTTGCGATGAATAACTGGTCCTTCCTGCAGTCAGGAAGTATTGGCATCGATCGCAAAGATATTGaa           <  1:331800/236‑1 (MQ=255)
                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                
TGCAGAAAGCAGACTCCTCCTTTCTTTATGATAAGTATCAGAATCAGAGTATTGCCGCGCATGTGATGCGCGCTCTCGCTGCTGAGCAGTCGGAAGTGTCGCCAGAACAGCGGCGCGCCATCTGCGAGGCTTTTGAGTCTGCCAATAACACCCATGGCTTAAACCTGACTGCCCATAAATACCCGGGCTTACGCGGCACACTACAAACCGCATCCACTGACTGCGACACAATTGTGGAAGCTGCAGCACTATTACCCGCTTTTGATCAGGCAGTGGAAGGCAACCGCCACCAGGATGATTACGGTTCAGGTCTTGGGATGGCCGAAGAGAAATTTCACTATTATCTCGATCTCAATGACCGCTATGTCTATTTTTATGAGCCGGTTAATGTTGAATACTTTGCGATGAATAACTGGTCCTTCCTGCAGTCAGGAAGTATTGGCATCGATCGCAAAGATATTGAAAAGGTATTT  >  NC_000913/1872168‑1872640

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: