Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	1,901,015	T→C	19.1%	S191G (AGC→GGC)	yoaE ←	putative membrane protein/conserved protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,901,015	0	T	C	19.1%	116.0 / 2.6	52	S191G (AGC→GGC)	yoaE	putative membrane protein/conserved protein
Reads supporting (aligned to +/- strand):  ref base T (23/17);  new base C (4/7);  total (27/25)
Fisher's exact test for biased strand distribution p-value = 3.10e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGGCATCGTGCTGAACATTGGCCTGACGTTTCCCGCCCATCAAACGCAGGATGGCATCGGCAGTACGCGCTCGCAGCGGCAAAGTCGACTGGTGGCGAATAAAGTTGCGACGCGCAATCTGGTTAAACACTTCGATGATGATCGAGAAGCCAATCGCGGCATACAGGTAACCTTTCGGA‑ATGTGGAAA‑CCGAAACCTTCTGCCACCAGACTCAGACCAATCATTAACAGGAAGCTCAGACA‑GAGCACCA‑CCACCGTGGGGTGCTGGTTAACGAATC‑GCGTCAGCGGTTTGGATGCCAGCAACATAACCGCCATCGCAATCACTACCGCCGCCATCATCACCGGCAGATGGTTAACCATCCCTACTGCAGTAATTACCGCATCCAAC‑‑GAGAAGACGGCGTCAAGGATGACAATCTGTGTGACGACCACCCAGAAACTGGCGTAGCCTTTACCGTGGCCGGAATCATG  >  NC_000913/1900781‑1901246                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                             cGGCATCGTGCTGAACATGGGCCTGGCGTTGCCCGCCCAACAACCGCAGGATGGCATCGGCAGTACGCGCTCGCAGCGGCAAAGTAGACTGGTGGCGAATAAAGTTGCGACGCGCAATCTGGTTAAACACTTCGATGATGATCGAGAAGCCAATCGCGGCATACAGGTAACCTTTCGGA‑ATGTGGAAA‑CCGAAACCTTCTGCCACCAGACTCAGACCAATCATTAACAGGAAGCTcaga                                                                                                                                                                                                                                          <  1:380947/239‑1 (MQ=255) cGGCAGCGTGCTGAACATGGGCCGGACGTTTCCCGCCCATCAAACGCAGGATGGCATCGGCAGTACGCGCTCGCAGCGGCAAAGTCGACTGGTGGCGAATAAAGTTGCGACGCGCAATCTGGTTAAACACTTCGATGATGATCGAGAAGCCAATCGCGGCATACAGGTAACCTTTCGGA‑ATGTGGAAA‑CCGAAACCTTCTGCCACCAGACTCAGACCAATCATTAACAGGAAGCTcaga                                                                                                                                                                                                                                          <  1:635721/239‑1 (MQ=255)         gtgggaccacgggCCCGGCGTTTCCCGCCCATCAAACGCAGGATGGGATCGGCATGACGCGCGCGCAGCGGCAAAGTCGACTGGTGGCGAATAAAGTTGCGACGGGCAATCTGGTTAAACACTTCGATGATGATCGAGAAGCCAATCGCGGCATACAGGTAACCTTTCGGA‑ATGTGGAAA‑CCGAAACCTTCTGCCACCAGACTCAGACCAATCATTAACAGGAAGCTCAGACA‑GAGcac                                                                                                                                                                                                                                 <  2:550655/228‑1 (MQ=255)              aaCATTGGCCTGACGTTTCCCGCCCATCAAACGCAGGATGGCATCGGCAGTACGCGCTCGCAGCGGCAAAGTCGACTGGTGGCGAATAAAGTTGCGACGCGCAATCTGGTTAAACACTTCGATGATGATCGGGAAGCCAATCGCGGCATACAGGTAACCTTTCGGA‑ATGGTGACA‑CCGAAACCTTCTGCCACCCACCACAAACCCATCATTAACAGGAAGCCccgaca‑gaaccacacc                                                                                                                                                                                                                             >  2:793634/1‑230 (MQ=255)               aCATTGGCCTGACGTTTCCCGCCCAGCAAACGCAGGATGGCATCGGCAGTACGCGCTCGCAGCGGCAAAGTCGACTGGTGGCGAATAAAGTTGCGACGCGCAATCTGGTTAAACACTTCGATGATGATCGAGAAGCCAATCGCGGCATACAGGTAACCTTTCGGA‑ATGTGGAAA‑CCGAACCCTTCTGCCACCAGACTCAGACCAATCATTAACAGGAAGCTCAGACA‑GAGcacca‑cca                                                                                                                                                                                                                           <  1:690443/238‑1 (MQ=255)                   tGGCCTGGCTGTTCCCGCCCAGAAAACGCAGGAGGGCATCGGCAGTACGCGCTCGCAGGGGAAAAGGCGACTGGTGGCGAATAAAGTTGCGACGCGCAATCTGGTTAAACACTTCGATGATGATCGAGAAGCCAATCGCGGCATACAGGTAACCTTTCGGA‑ATGTGGAAA‑CCGAAACCTTCTGCCACCAGACTCAGACCAATCATTAACAGGAAGCTCAGACA‑GAGCACCA‑CCACCGTg                                                                                                                                                                                                                      <  2:82674/239‑1 (MQ=255)                   tGGCCTGACGTTTCCCGCCCATCAAACGCAGGATGGCATCGGCAGTACGCGCTCGCAGCGGCAAAGTCGACTGGTGGCGAATAAAGTTGCGACGCGCAATCTGGTTAAACACTTCGATGATGATCGAGAAGCCAATCGCGGCCTACAGGAAACCTTTCGGA‑ATTTGGAAA‑CCGAAACCTTCTCCCCCCAGCACCACACCCACCCTTAACAGAGAACCCACACA‑GAg‑acca‑ccaaccccg                                                                                                                                                                                                                     >  2:50478/1‑233 (MQ=255)                   tGGCCTGACGTTTCCCGCCCATCAAACGCAGGATGGCATCGGCAGTACGCGCTCGCAGCGGCAAAGTCGACTGGTGGCGAATAAAGTTGCGACGCGCAATCTGGTTAAACACTTCGATGATGATCGAGAAGCCAATCGCGGCATACAGGTAACCTTTCGGA‑ATGTGGAAA‑CCGAAACCTTCTGCCACCAGACCCAGCCCAACCATTAACAGGAACCCCAAAAA‑GAACACCA‑CCACCCTg                                                                                                                                                                                                                      >  2:1042752/1‑239 (MQ=255)                        tgaactttCCCGCCCATCAAACGGAGGATGGCATCGGCAGTACGCGCTCGCAGCGGCAAAGGCGACTGGGGGCGAATAAAGTTGCGACGCGCAATCTGGTTAAACACTTCGATGATGATCGAGAAGCCAATCGCGGCATACAGGTAACCTTTCGGA‑ATGTGGAAA‑CCGAAACCTTCTGCCACCAGACTCAGACCAATCATTAACAGGAAGCTCAGACA‑GAGCACCA‑CCACCGTGGGGTg                                                                                                                                                                                                                 <  1:974699/234‑1 (MQ=255)                        tGACGTTTCCCGCCCATCAAACGCAGGATGGCATCGGCAGTACGCGCTCGCAGCGGCAAAGTCGACTGGTGGCGAATAAAGTTGCGACGCGCAATCTGGTTAAACACTTCGATGATGATCGAGAAGCCAATCGCGGCATACAGGTAACCTTTCGGA‑ATGTGGAAA‑CCGAAACCTTCTGCCACCCGCCTCAGACCAATCATTAACAGGAAGCTCAGACA‑GGGCACCA‑CCACCGAGGGGt                                                                                                                                                                                                                  >  1:844227/1‑238 (MQ=255)                        tGACGTTTCCCGCCCATCAAACGCAGGATGGCATCGGCAGTACGCGCTCGCAGCGGCAAAGTCGACTGGTGGCGAATAAAGTTGCGACGCGCAATCCGGTTAAACACTTCGATGATGATCGAGAAGCCAATCGCGGCATACAGGTAACCTTTCGGA‑ATGTGGAAA‑CCGAAACCTTCTGCCACCCAACCCAAACCAATCATTAAAAAGAAACCCAGACA‑AAGCACCC‑CCACCGTgggggg                                                                                                                                                                                                                 >  2:1046396/1‑237 (MQ=255)                          aCGTTTCCCGCCCATCAAACGCAGGATGGCATCGGCAGTACGCGCTCGCAGCGGCAAAGTCGACTGGTGGCGAATAAAGTTGCGACGCGCAATCTGGTTAAACACTTCGATGATGATCGAGAAGCCAATCGCGGCATACAGGTAACCTTTCGGA‑ATGTGGAAA‑CCGAAACCTTCTGCCACCCGACTCAGACCAATCATTAACAGGACGCTCAGACA‑GAGCACCA‑CCACCGCGGGGt                                                                                                                                                                                                                  >  1:807064/1‑236 (MQ=255)                          aCGTTTCCCGCCCATCAAACGCAGGATGGCATCGGCAGTACGCGCTCGCAGCGGCAAAGTCGACTGGTGGCGAATAAAGTTGCGACGCGCAATCTGGTTAAACACTTCGATGATGATCGAGAAGCCAATCGCGGCATACAGGTAACCTTTCGGA‑ATGTGGAAA‑CCGAAACCTTCTGCCACCAGACTCAGACCAATCATTAAAAGGAAGCTCAGACA‑GAGACCCA‑CCACCGGGGGGGGcg                                                                                                                                                                                                               >  1:928256/1‑238 (MQ=255)                                    cccATCAAACGCAGGATGGCATCGGCAGTACGCGCTCGCAGCGGCAAAGTCGACTGGTGGCGAATAAAGTTGCGACGCGCAATCTGGTTAAACACTTCGATGATGATCGAGAAGCCAATCGCGGCATACAGGTAACCTTTCGGA‑ATGTGGAAA‑CCGAAACCTTCTGCCACCAGACTCAGACCACTCATTAAAAGGAAGCTCCGACA‑GAGCACCA‑CCACCGTGGGGTGCTGGGTAACGa                                                                                                                                                                                                      >  1:163340/1‑238 (MQ=255)                                    cccATCAAACGCAGGATGGCATCGGCAGTACGCGCTCGCAGCGGCAAAGTCGACTGGTGGCGAATAAAGTTGCGACGCGCAATCTGGTTAAACACTTCGATGATGATCGAGAAGCCAATCGCGGCATACAGGTAACCTTTCGGA‑ATGTGGAAA‑CCGAAACCTTCTGCCACCAGACTCAGACCAATCATTAACAGGAAGCTcagaca‑g                                                                                                                                                                                                                                      <  1:76467/207‑1 (MQ=255)                                    cccATCAAACGCAGGATGGCATCGGCAGTACGCGCTCGCAGCGGCAAAGTCGACTGGTGGCGAATAAAGTTGCGACGCGCAATCTGGTTAAACACTTCGATGATGATCGAGAAGCCAATCGCGGCATACAGGTAACCTTTCGGA‑ATGTGGAAA‑CCGAAACCTTCTGCCACCAGACTCAAGCCAATCATTAACAGGAAGCTcagaca‑g                                                                                                                                                                                                                                      >  2:76467/1‑207 (MQ=255)                                         cAAACGCAGGATGGCATCGGCAGTACGCGCTCGCAGCGGCAAAGTCGACTGGTGGCGAATAAAGTTGCGACGCGCAATCTGGTTAAACACTTCGATGGTGATCGAGAAGCCAATCGCGGCATACAGGGAACCTTTCGGA‑ATGTGGAAA‑CCGAAACCTTCTGCCACCAGACTCAGACCAATCATTAACAGGAAGCTCAGACA‑GAGAACCA‑CCCCCGTGGGGTGCGGGCTAACGAACc‑gcg                                                                                                                                                                                               >  1:667632/1‑239 (MQ=255)                                           aaCGAAGGATGGGATCGGCAGTTAGCGACCGCAGCGGCAAAGTCGACTGGTGGCGAATAAAGTTGCGACGCGCAATCTGGTTAAACACTTCGATGATGATTGAGAAGCCAATCGCGGCATACAGGTAACCTTTCGGA‑ATGTGGAAA‑CCGAAACCTTCTGCCACCAGACTCAGACCAATCATTAACAGGAAGCTCAGACA‑GAGCACCA‑CCACCGTGGGGTGCTGGTTAACGAATC‑GCGTc                                                                                                                                                                                             <  1:1083115/239‑1 (MQ=255)                                                     ggCATCGGCAGTACGCGCTCGCAGCGGCAAAGTCGACTGGTGGCGAATAAAGTTGCGACGCGCAATCTGGTTAAACACTTCGATGATGATCGAGAAGCCAATCGCGGCATACAGGTAACCTTTCGGA‑ATGTGGAAA‑CCGAAACCTTCTGCCACCAGACTCAGACCAATCATTAACAGGAAGCTCAGACA‑GAGCACCA‑CCACCGTGGGGTGCTGGTTAACGAATC‑GCGTCAGCGGTTTgg                                                                                                                                                                                   <  1:284403/239‑1 (MQ=255)                                                          cGGGAGTAGGCGCGCGGAGTGGGAAAGGCCGGTGGTGGCGAACAAAGTGGGGACGCGCAATCTGGTTAAACACTTCGAGGATGATCGAGAAGCCAATCGCGGCATACAGGTAACCTTTCGGA‑ATGTGGAAA‑CCGAAACCTTCTGCCACCAGACTCAGACCAATCATTAACAGGAAGCTCAGACA‑GAGCACCA‑CCACCGTGGGGTGCTGGTTAACGAATC‑GCGTCAGCGGTTTGGATGcc                                                                                                                                                                              <  2:446597/239‑1 (MQ=255)                                                             cAGTACGCGCTCGCAGGGGCAAAGTCGACTGGTGGCGAATAAAGTTGCGACGCGCAATCTGGTTAAACACTTCGATGATGATCGAGAAGCCAATCGCGGCATACAGGTAACCTTTCGGA‑ATGTGGAAA‑CCGAACCCTTCTGCCACCAGACTCAGACCAATCATTAACAGGAAGCTCAGACA‑GAGCACCA‑CCTCCGTGGGGTGCTGGTTAACGAATC‑GCGTCAGCGGTTTGGATGCCAg                                                                                                                                                                            <  1:793634/238‑1 (MQ=255)                                                                    cggtCGCAGCGGCAAAGGCGACTGGTGGCGAATAAAGTTGCGACGCGCAATTTGGTTAAACACTTCGATGATGATCGAGAAGCCAATCGCGGCATACAGGTAACCTTTCGGA‑ATGTGGAAA‑CCGAAACCTTCTGCCACCAGACTCAGACCAATCATTAACAGGAAGCTCAGACA‑GAGCACCA‑CCACCGTGGGGTGCTGGTTAACGAATC‑GCGTCAGCGGTTTGGATGCCAGCAACAt                                                                                                                                                                      <  1:1046396/234‑1 (MQ=255)                                                                              gggaaaGTCGACTGGGGGCGAATAAAGTTGCGACGCGCAATCTGGTTAAACACTTCGATGATGATCGAAAAGCCAATCGCGGCATACAGGTAACCTTTCGGA‑ATGTGGAAA‑CCGAAACCTTCTGCCCCCAGACTCAGACCAATCATTAACAGGAAGCTCAGACA‑GAGCACCA‑CCACCGTGGGGTGCTGGTTAACGAATC‑GCGTCAGCGGTTTGGATGCCAGCAACATAACCGCCATc                                                                                                                                                            <  1:1084446/234‑1 (MQ=255)                                                                                            gtggCGAATAAAGTTGCGACGCGCAATCTGGTTAAACACTTCGATGATGATCGAGAAGCCAATCGCGGCATACAGGTAACCTTTCGGA‑ATGTGGAAA‑CCGAAACCTTCTGCCACCAGACTCAGACCAATCATTAACAGGAAGCTCAGACA‑GAGCACCA‑CCACCGTGGGGTGCTGGTTAACGAATC‑GCGTCACCGGTTTGGATGCCAGCACCATAACCCCCATCGCAAtacatacctcc                                                                                                                                             >  1:953057/1‑228 (MQ=255)                                                                                               gCGAATAAAGTTGCGACGCGCAATCTGGTTAAACACTTCGATGATGATCGAGAAGCCAATCGCGGCATACAGGTAACCTTTCGGA‑ATGTGGAAA‑CCGAAACCTTCTGCCCCCAGACTCAGACCAATCATTAACAGGAAGCTCAGACA‑GAGCACCA‑CCACCGTGGGGGGCTGGTTAACGAATC‑GCGTCAACGGTTTGGATGCACGCAAAATAACCGCCATCGCAATCACAACCGCCCCca                                                                                                                                         >  2:533983/1‑239 (MQ=255)                                                                                                                  gtaaTTTGGTTAACCACTTCGATGATGATGGAGAAGCCAACCGGGGCATACAGGTAACCTTTGGGA‑ATGTGGAAC‑CCGAAACCTCTGGCCACCAGACCAAGACCAATCATTAACAGGAAGCCCAGACA‑GAGCACCA‑CCACCGTGGGGTGCTGGTTAACGAATC‑GCGTCAGCGGTTTGGATGCCAGCAACATAACCGCCATCGCAATCACTACCGCCGCCATCATCACCGGCAGATGGt                                                                                                                       <  2:933631/236‑1 (MQ=255)                                                                                                                           ttAAACACTTCGATTATGCGCGGAAAGCCAATCGCGGCATACAGGTACCCTTTCGGA‑ATGTGGAAA‑CCGAAACCTTCTGCCACCAGCCTCAGACCAATCATTAACAGGAAGCTCAGACA‑GAGCACCA‑CCACCGTGGGGTGCTGGTTAACGAATC‑GCGTCAGCGGTTGGGATGCCAGCAACATAACCGCCATCGCAATCACTACCGCCGCCATCATCACCGGCAGATGGTTAACCATccc                                                                                                             <  1:1085250/239‑1 (MQ=255)                                                                                                                             aaccccTTCGATGATGAACGAAAAGCCAATCGCGGCATACAGGTAACCTTTCGGA‑ATGGGGAAA‑CCGAACCCTTCGGCCACCAGACTCAGACCAATCATTAACAGGAAGCCCAGACA‑GAGCACCA‑CCACCGTGGGGTGCTGGTTAACGAATC‑GCGTCAGCGGTTTGGATGCCAGCAACATAACCGCCATCGCAATCACTACCGCCGCCATCATCACCGGCAGATGGTTAACCATCCCTa                                                                                                           <  1:915017/234‑1 (MQ=255)                                                                                                                             aaaCACTTGGATGATTAACGAGAACCCAATCGGGGCATACAGGTAACCTTTCGGA‑ATGTGGAAA‑CCGAAACCTTCCGCCCCCAGACTCAGACCAATCATTAACAGGAAGCTCAGACA‑GAGCACCA‑CCACCGGGGGGTGCTGGTTAACGAATC‑GCGTCAGCGGTTTGGATGCCAGCAACATAACCGCCATCGCAATCACTACCGCCGCCATCATCACCGGCAGATGGTTAACCATCCCTa                                                                                                           <  1:867782/239‑1 (MQ=255)                                                                                                                              aaCACTTCGATGATGATCGAGAAGCCAATCGCGGCATACAGGTAACCTTTCGGA‑ATGTGGAAA‑CCGAAACCTTCTGCCACCAGACTCAGACCAATCATTAACAGGAAGCTCAGACA‑GAGCACCA‑CCACCGTGGGGTGCTGGTTAACGAATC‑GCGTCAGCGGTTTGGATGCCAGCAACATAACCGCCATCGCAATCCCTACCGCCGCCATCATCACCGGCAGATGGTTAACCATcccaa                                                                                                           >  1:527950/1‑236 (MQ=255)                                                                                                                                    tCGCTGATGATCGAGAAGCCAATCGCGGCATACAGGTAACCTTTCGGA‑ATGTGGAAA‑CCGAAACCTTCTGCCACCAGACTCAGACCAATCATTAACAGGAAGCTCAGACA‑GAGCACCA‑CCACCGTGGGGTGCTGGTTAACGAATC‑GCGTCAGCGGTTTGGATGCCAGCAACATAACCGCCATCGCAATCACTACCGCCGCCATCATCACCGGCAGATGGTTAACCATCCCTACTGCCGt                                                                                                    >  1:967841/1‑239 (MQ=255)                                                                                                                                    tCGATGATGATCGAGAAGCCAATCGCGGCATACAGGTAACCTTTCGGA‑ATGTGGAAA‑CCGAAACCTTCTGCCACCAGACTCAGACCAATCATTAACAGGAAGCTCAGACA‑GAGCACCA‑CCACCGTGGGGTGCTGGTTAACGAATC‑GCGTCAGCGGTTTGGATGCCAGCAACATAACCGCCATCGCAATCACTACCGCCGCCATCATCACCGGCAGATGGTTAACCATCCCTACTGCAGt                                                                                                    >  1:1006214/1‑239 (MQ=255)                                                                                                                                           ggataggaaaGCAAATCGCGGAATACAGGTAACCTTCCGGA‑ATGTGGAAC‑CCGAAACCTTCTCCCCCCAGACTCAGACCAATCATTACCAGGAAGCTCAGACA‑GAGCACCA‑CCACCGTGGGGTGCTGGTTAACGAATC‑GCGTCAGCGGTTTGAATGCCAGCAACATAACCGCCATCGCAATCACTACCGCCGCCATCATCACCGGCAGATGGTTAACCATCCCTACTGCAGTAATTAc                                                                                              <  1:1042752/230‑1 (MQ=255)                                                                                                                                                        aaTCGCGGCATACAGGTAACCTTTCGGA‑ATGTGGAAA‑CCGAAACCTTCTGCCACCAGACTCAGACCAATCATTAACAGGAAGCTCAGACA‑GAGCACCA‑CCACCGTGGGGTGCTGGTTAACGAATC‑GCGTCAGCGGTTTGGATGCCAGCAACATAACCGCCATCGCAATCACCACCGCCGCCACCATCCCCGGCAGAGGGTTAACCACCCCAACCCCAGAAAATACCCACTCCAAA‑‑gaga                                                                              >  2:995618/1‑239 (MQ=255)                                                                                                                                                          tCGCGGCATACAGGTAACCTTTCGGA‑ATGTGGAAA‑CCGAAACCTTCTGCCACCAGACTCAGACCAATCATTAACAGGAAGCTCAGACA‑GAGCACCA‑CCACCGTGGGGTGCTGGTTAACGAATC‑GCGTCAGCGGTTTGGATGCCAGCAACATAACCGCCATCGCAATCACTACCGCCGCCATCATCACCGGCACATGGTTAACCATCCCTACTGCAGTAATTACCCCACCCAAC‑‑Gaaaaa                                                                            >  2:154342/1‑238 (MQ=255)                                                                                                                                                                        ttcccTTTCGGA‑ATTGGGAAA‑CCGAACCCCTCCCCCACACACCTCAGACAAACCTATAACGGGACTCCCAGACA‑GACCACCC‑CCACCGTGGGGTGCTGGTTAACGAATC‑GCGTCAGCGTTTTGGATCCCAGCAACATAACCGCCATCGCAATCACTACCGCCGCCATCATCACCGGCAGATGGTTAACCATCCCTACTGCAGTAATTACCGCATCCAAC‑‑GAGAAGACGGCGTCAAGGAt                                                              <  2:928256/236‑1 (MQ=255)                                                                                                                                                                        tAACCTTCAGGA‑ATGTGGAAA‑CCGAACCCTTCTGCCCCCCGCCTCAGACCAATCATTAACAGGAAGCCCAGCAG‑GAGCACCA‑CCCCCGTGGGGTGCTGGTTAACGAATC‑GCGTCAGCGGTTTGGATGCCAGCAACATAACCGCCATCGCAATCACTACCGCCGCCATCATCACCGGCAGATGGTTAACCATCCCTACTGCAGTAATTACCGCATCCAAC‑‑GAGAAGACGGCGTCAAgg                                                                <  1:533983/237‑1 (MQ=255)                                                                                                                                                                         taCCCTTCCGG‑ATGTGGAAACCCGAAACCTCCTGCCACCAGACCAAGCCCAACCATAACCAGGAACCCAAGAC‑‑GAGCCCCACCCGCCGTGGGGTGCGGTTTAACGAATC‑GGGCCGCGGGTTGGGATGCCACCAACATACCCGCCATCGCAATCACTACCGCCGCCATCATCACCGGCAGATGGTTAACCATCCCTACTGCAGTAATTACCGCATCCAAC‑‑GAGAAGACGGCGTCAAGGAt                                                              <  2:809005/238‑1 (MQ=255)                                                                                                                                                                          aCCTTTCGGA‑ATGTGGAAA‑CCGAAACCTTCTGCCACCAGACTCAGACCAATCATTAACAGGAAGCTCAGACA‑GAGCACCA‑CCACCGTGGGGTGCTGGTTAACGAATC‑GCGTCAGCGGTTTGGATGCCAGCAACATAACCGCCATCGCAATCACTACCGCCGCCCTCATCACCGGCAGATGGTTAACCATCCCTCCTGCAGTAATTACCGCATCCAAC‑‑GCGAAGCCGGctcacaggatg                                                             >  1:576117/1‑228 (MQ=255)                                                                                                                                                                            cTTTCGGAGATGTGGAAA‑CGAAACCCGTGTCCCACCGCACCGAGCCCAATAATGAACAGGAGACGCGGAAA‑GGGCCCCC‑CCCCGGGGGGGTGCGGGTTAACGAATC‑GGGTCAGCGGTTGGAATGCCAGCAACATAACCGCCATCGCAATCACTACCGCCGCCATCATCACCGGCAGATGGTTAACCATCCCTACTGCAGTAATTACCGCATCCAAC‑‑GAGAAGACGGCGTCAAGGATGAc                                                           <  2:501251/239‑1 (MQ=255)                                                                                                                                                                               tCGGA‑ATGTGGAAA‑CCGAAACCTTCTGCCACCAGACTCAGACCAATCATTAACAGGAAGCTCAGACA‑GAGCACCA‑CCACCGTGGGGTGCTGGTTAACGAATC‑GCGTCAGCGGTTTGGATGCCAGCAACATAACCGCCATCGCAATCACTACCGCCGCCATCATCACCGGCAGCTGGTTAACCATCCCTACTGCAGTAATTACCGCATCCAAC‑‑GAGAAAACGACGTCAAGGAAGACaa                                                         >  1:539455/1‑237 (MQ=255)                                                                                                                                                                               tCGGA‑ATGTGGAAA‑CCGAAACCTTCTGCCACCAGACTCAGACCAATCATTAACAGGAAGCTCAGACA‑GAGCACCA‑CCACCGTGGGGTGCTGGTTAACGAATC‑GCGTCAGCGGTTTGGATGCCAGCAACATAACCGCCATCGCAATCACTACCGCCGCCATCATCACCGGCAGATGGTTAACCATCCCTACTGCAGTAATTACCGCATCCAAC‑‑GAGAAGCAGGCGTCAAGGAGGACaacc                                                       >  2:691727/1‑237 (MQ=255)                                                                                                                                                                               tCGGA‑ATGTGGAAA‑CCGAAACCTTCTGCCACCAGACTCAGACCAATCATTAACAGGAAGCTCAGACA‑GAGCACCA‑CCACCGTGGGGTGCTGGTTAACGAATC‑GCGTCAGCGGTTTGGATGCCAGCAACATAACCGCCATCGCAATCACTACCGCCGCCATCATCACCGGCAGATGGTTAACCATCCCTACTGCAGTAATTACCGCATCCAAC‑‑ACAAAGAAGGCGTCAACGATGCCaa                                                         >  1:330698/1‑237 (MQ=255)                                                                                                                                                                                                   aaccTTCTGCCACCAGACTCAGACCAATCATTAACAGGAAGCTCAGACA‑GAGCACCA‑CCACCGTGGGGTGCTGGTTAACGAATC‑GCGTCAGCGGTTTGGATGCCAGCAACATAACCGCCATCGCAATCACTACCGCCGCCATCATCACCGGCAGATGGTTAACCATCCCTACTGCAGTAATTACCGCATCCAAC‑‑GAGAAGCAGGCGTCAAGGTTGACAATCTGTGTGCCGACCACCCaa                                     >  1:378853/1‑238 (MQ=255)                                                                                                                                                                                                          tGCCACCAGACTCAGACCAATCATTAACAGGAAGCTCAGACA‑GAGCACCA‑CCACCGTGGGGTGCTGGTTAACGAATC‑GCGTCAGCGGTTTGGATGCCAGCAACATAACCGCCATCGCAATCACTACCGCCGCCATCATCACCGGCAGATGGTTAACCATCCCTACTGCAGTAATTACCGCATCCAAC‑‑GAGAAGACGGCGTCAAGGATGACAATCTGTGTGACGACCACCCAGAAACTg                               >  1:212925/1‑238 (MQ=255)                                                                                                                                                                                                           gccccaggACTCAGCCCAATCATTAACAGGAAGCTCA‑ACACGGCCACCA‑CCACCGTGGGGTGCTGGTTAACGAACC‑GCGTCAGCGTTTTGGATGCCAGCAAAATAACCGCCATCGCAATCACTACCGCCGCCATCATCACCGGCAGATGGTTAACCATCCCTACTGCAGTAATTACCGCATCCAAC‑‑GAGAAGACGGCGTCAAGGATGACAATCTGTGTGACGACCACCCAGAAACTGGc                             <  1:691727/232‑1 (MQ=255)                                                                                                                                                                                                                        gACCAATCATTAACAGGAAGCTCAGACA‑GAGCACCA‑CCACCGTGGGGTGCTGGTTAACGAATC‑GCGTCAGCGGTTTGGATGCCAGCAACATAACCGCCATCGCAATCACTACCGCCGCCATCATCACCGGCAGATGGTTAACCATCCCTACTGCAGTAATTACCGCATCCAAC‑‑GAGAAGACGGCGTCAAGGATGACaa                                                         >  1:885940/1‑198 (MQ=255)                                                                                                                                                                                                                            cacaagtAACGGGAATCCCAGACG‑GGCCACCA‑CCCCTGTGGGG‑GGTGGGTAAAGAAACGGCGTAGGCGTGTTGAAGCGCACCAAAATACCCGCCATCGCAATCACTCCCGCCGCCATCATCACCGGCAGATGGTTAACCATCCCTACTGCAGTAATTACCGCATCCAAC‑‑GAGAAGACGGCGTCAAGGATGACAATCTGTGTGACGACCACCCAGAAACTGGCGTAGCCTTTACCGTGGc            <  2:967841/233‑1 (MQ=255)                                                                                                                                                                                                                            aaccATTAACAGGAAGCCAAGACA‑GGCCACCA‑CCCCGGTGGGGTGCTGGTTACAGAACC‑GCGTCAGCGGTTTGGATGCCAGCAACATACCCGCCATCGCAATCACTACCGCCGCCATCATCACCGGCAGATGGTTAACCATCCCTACTGCAGTAATTACCGCATCCAAC‑‑GAGAAGACGGCGTCAAGGATGACAATCTGTGTGACGACCACCCAGAAACTGGCGTAGCCTTTACCGTGGc            <  1:180152/236‑1 (MQ=255)                                                                                                                                                                                                                             aTCATTAACAGGAAGCTCAGACA‑GAGCACCA‑CCACCGTGGGGTGCTGGTTAACGAATC‑GCGTCAGCGGTTTGGATGCCAGCAACATAACCGCCATCGCAATCACTACCGCCGCCATCATCACCGGCAGATGGTTAACCATCCCTACTGCAGTAATTACCGCATCCACC‑‑GAGAAGACGGCGTCAAGGATGACAATCTGTGTGACGACCCCCCAGAAACCGGCGTACCCTTTACCTTGGcg           >  1:1054442/1‑238 (MQ=255)                                                                                                                                                                                                                                     cAGGCAGCTCAGACA‑GAGCACCA‑CCACCGTGGGGTGCTGGTTAACGAATC‑GCGTCAGCGGTTTGGATGCCAGCAACATAACCGCCATCGCAATCACTACCGCCGCCATCATCACCGGCAGATGGTTAACCATCCCTACTGCAGTACTCACCGCATCCACC‑‑GAGAAGACGGCGTCAAGGATTACAATATTAGGGACGACCACCCCGAAACCGGCGTAACCTTTTACGAGGCCgggaacaa   >  2:188651/1‑233 (MQ=255)                                                                                                                                                                                                                                        gAAGCTCAGACA‑GAGCACCA‑CCACCGTGGGGTGCTGGTTAACGAATC‑GCGTCAGCGGTTTGGATGCCAGCAACATAACCGCCATCGCAATCACTACCGCCGCCATCATCACCGGCAGATGGTTAACCATCCCTACTGCAGTAATTACCGCATCCAACGAGAGAAGACGGCGTCAAGGATGACAATCTGTGTGACGACCACCCAGAAACTGCCGTAGCCTTTACCGTGGCCGGAAACATg  >  1:995959/1‑239 (MQ=255)                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                             CGGCATCGTGCTGAACATTGGCCTGACGTTTCCCGCCCATCAAACGCAGGATGGCATCGGCAGTACGCGCTCGCAGCGGCAAAGTCGACTGGTGGCGAATAAAGTTGCGACGCGCAATCTGGTTAAACACTTCGATGATGATCGAGAAGCCAATCGCGGCATACAGGTAACCTTTCGGA‑ATGTGGAAA‑CCGAAACCTTCTGCCACCAGACTCAGACCAATCATTAACAGGAAGCTCAGACA‑GAGCACCA‑CCACCGTGGGGTGCTGGTTAACGAATC‑GCGTCAGCGGTTTGGATGCCAGCAACATAACCGCCATCGCAATCACTACCGCCGCCATCATCACCGGCAGATGGTTAACCATCCCTACTGCAGTAATTACCGCATCCAAC‑‑GAGAAGACGGCGTCAAGGATGACAATCTGTGTGACGACCACCCAGAAACTGGCGTAGCCTTTACCGTGGCCGGAATCATG  >  NC_000913/1900781‑1901246

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑