Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	216,077	G→C	5.2%	intergenic (+98/+102)	nlpE → / ← yaeF	lipoprotein involved with copper homeostasis and adhesion/putative lipoprotein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	216,077	0	G	C	5.2%	273.0 / 2.7	94	intergenic (+98/+102)	nlpE/yaeF	lipoprotein involved with copper homeostasis and adhesion/putative lipoprotein
Reads supporting (aligned to +/- strand):  ref base G (40/47);  new base C (2/4);  total (43/51)
Fisher's exact test for biased strand distribution p-value = 6.86e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.32e-01

GAAAAACCGGTGTTACTGTCAGTAGAAGGTCACTTTACGCTTGAGGGTAATCCGGATACCGGTGCGCCGACTAAAGTATTGGCACCCGATACGGCAGGG‑AAATTTTACCCCAACCAGGATTGCAGTAGTTTGGGGCAGTAACCCGTCTTG‑AGACAGAAACAAACGCAAAACAGGCCAGAA‑GGATATATTTCAACAT‑TTTGAATTTGCACGTTTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCG‑GCAATGGTGCTCAACGCCTGAT‑GCGACGCTGGCGCGTCTTATCATGCCT‑ACA‑ACCCCCCTCATACCTTAAGGCGGATAAGGCATTTACTTTATCAC‑CGGGTCAAACC‑AACAAAGCGACCCGCCTTGATATAAATCCCAGGCTTCAGGTGCCCAACATACTGTAACTGCGTTTCTGGCTTAAACGCAGAGACATCACCAATACGCATATGCATCAGATCGGCAGGGC  >  NC_000913/215839‑216312                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                  gAAAAACCGGTGTTACTGTCAGTAGAAGGTCACTTTACGCTTGAGGGTAATCCGGATACCGGTGCGCCGACTAAAGTATTGGCACCCGATACGGCAGGG‑AAATTTTACCCCAACCAGGATTGCAGTAGTTTGGGGCAGTAACCCGTCTTG‑AGACAGAAACAAACGCAAAACAGGCCAGAA‑GGATATATTTCAACAT‑TTTGAATTTGCACGTTTTTTGTAGGCCGGATAAGGCGTTTAcg                                                                                                                                                                                                                                                   <  1:443259/239‑1 (MQ=255) gAAAAACCGGTGTTACTGTCAGTAGAAGGTCACTTTACGCTTGAGGGTAATCCGGATACCGGTGCGCCGACTAAAGGTTTGGCACCCGATACGGCAGGG‑AAATTTTACCCCAACCAGGATTGCAGTAGTTTGGGGCAGTAACCCGTCTTG‑AGACAGAAACAAACGCAAAACAGGCCAGAA‑GGATATATTTCAACAT‑TTTGAATTTGCACGTTTTTTGTAGGCCGGATAAGGCGTTTAcg                                                                                                                                                                                                                                                   <  1:1072947/239‑1 (MQ=255)    aaaCCGGTGTTACTGTCAGTAGAAGGTCACTTTACGCTTGAGGGTAATCCGGATACCGGTGCGCCGACTAAAGTATTGGCACCCGATACGGCAGGG‑AAATTTTACCCCAACCAGGATTGCAGTAGTTTGGGGCAGTAACCCGTCTTG‑AGACAGAAACAAACGCAAAACAGGCCCGAA‑GGAGATATTTCAACAT‑TTTGAATTTGCACGTTTTTTTTAGGCCGGAAAAGGCGTTTAcgccg                                                                                                                                                                                                                                                >  1:486184/1‑239 (MQ=255)      cccGGTTTTTCTTTCAGTAGAAGGTCACTTTACGCTTGAGGGTAATCCGGATACCGGTTCGCCGACTAAAGTATTGGCACCCGATACGGCAGGG‑AAATTTTACCCCAACCAGGATTGCAGTAGTTTGGGGCAGTAACCCGTCTTG‑AGACAGAAACAAACGCAAAACAGGCCAGAA‑GGATATATTTCAACAT‑TTTGAATTTGCACGTTTTTTGTAGGCCGGATAAGGCGTTTACGCCGCa                                                                                                                                                                                                                                              <  2:5058/238‑1 (MQ=255)            gtTGCTGGCAGTAGAAGGCCATTTTACGCTTGAGGGTAATCCGGATTCCGGTGCGCCGACTAAAGTATTGGCACCCGATACGGCAGGG‑AAATTTTACCCCAACCAGGATTGCAGTAGTTTGGGGCAGTAACCCGTCTTG‑AGACAGAAACAAACGCAAAACAGGCCAGAA‑GGATATATTTCAACAT‑TTTGAATTTGCACGTTTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCg‑g                                                                                                                                                                                                                                        <  1:290771/238‑1 (MQ=255)                     aGTAGAAGGTCACTTTACGCTTGAGGGTAATCCGGATACCGGTGCGCCGACTAAAGTATTGGCACCCGATACGGCAGGGAAAATTTTACCCC‑ACCACGATTTCAGAAGTTTGGGGCCGACACCCGTCTTT‑AGAAACAAACAAACGCAAAAAAGGGCACAA‑GAGTATTTTTCAACAT‑TTTGAAATTTCACGTTTTTTGTATGCCGGGTAAGGCGTTTACCCCCCATcccgacatggggg                                                                                                                                                                                                                                >  2:651695/1‑227 (MQ=255)                            cgtccatttACGGTTGAGGGTAACCCGGATACGGGTGCGGCGACTAAAGTATTGGCCCCCGATACGGCAGGG‑AAATTTTACCCCAACCAGGATTGCAGTAGTTTGGGGCAGTAACCCGTCTTG‑AGACAGAAACAAACGCAAAACAGGCCAGAA‑GGATATATTTCAACAT‑TTTGAATTTGCACGTTTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCG‑GCAATGGTGCTCAACGcc                                                                                                                                                                                                                       <  2:31580/233‑1 (MQ=255)                             gTCACTTTACGCTTGAGGGTAATCCGGATACCGGTGCGCCGACTAAAGTATTGGCACCCGATACGGCAGGG‑AAATTTTACCCCAACCAGGATTGCCGTAGTTTGGGGCAGTAACCCGTCTTG‑AGACAGAAACAAACGCAAAACAGGCCCGAA‑GGATCTATTTCAACAT‑TTTGAATTTGCACGGTTTTTTTAGGCCGGGTAAGGCGGTTACGCCCCATCCG‑GCAATTGGGCTCAAcc                                                                                                                                                                                                                         >  1:87676/1‑235 (MQ=255)                              ttaCTTTACGCTTGAGGGTAACCCGGATACCGGTGCGCCGACTAAAGTATTGGCCCCCGATACGGCAGGG‑AAATTTTACCCCAACCAGGATTGCAGTAGTTTGGGGCAGTAACCCGTCTTG‑AGACAGAAACAAACGCAAAACAGGCCAGAA‑GGATATATTTCAACAT‑TTTGAATTTGCACGTTTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCG‑GCAATGGTGCTCAACGcc                                                                                                                                                                                                                       <  1:978030/235‑1 (MQ=255)                                 cTTTACGCTTGAGGGTAATCCGGATACCGGTGCGCCGACTAAAGTATTGGCACCCGATACGGCAGGG‑AAATTTTACCCCAACCAGGATTGCAGCAGTTTGGGGCAGCAACACGACTTG‑TGACAGCAACAAACGCAAAAAACGCCGACA‑GGATAAATTTTAACAA‑TTAGTATTTTCTCGGTTTTTTTAGTCCCGGTAAAGCGTTTACACGCCATCCG‑CCAATAGGGTTCAAACCCTgtg                                                                                                                                                                                                                   >  2:207217/1‑236 (MQ=255)                                           gAGGGTACTCCGGTTACCGGTGCCCCGATCAAAGTATTGGCACCCGATACGGCAGGG‑AAATTTTACCCCACCCAGGATTGCAGTAGTTTGGGGCAGTAACCCGTCTTG‑AGACAGAAACAAACGCAAAACAGGCCAGAA‑GGATATATTTCAACAT‑TTTGAATTTGCACGTTTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCG‑GCAATGGTGCTCAACGCCTGAT‑GCGACGCTGgc                                                                                                                                                                                                       <  2:533569/239‑1 (MQ=255)                                           gAGGGTAATCCGGATTCCGGTGCGACGACTAAAGTATTGGCACCCGATACGGCAGGG‑AAATTTTGCCCCAACCAGGATTGCAGTAGGTTGGGGCAGTAACCCGTCTTG‑AGACAGAAACAAACGCAAAACAGGCCAGAA‑GGATATATTTCAACAT‑TTTGAATTTGCACGTTTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCG‑GCAATGGTGCTCAACGCCTGAT‑GCGACGCTGgc                                                                                                                                                                                                       <  1:442567/239‑1 (MQ=255)                                            aGGGTAATCCGGATACCGGTGCGCCGACTAAAGTATTGGCACCCGATACGGCAGGG‑AAAATTTACCCCAAACAGGGTTGCAGTAGTTTGGGGCAGTAACCCGTCTTG‑AGACAAAAACACACCAAAAACAGGCCAGAA‑GGGTATATTTCAACAT‑TTTGAATTTGCACGATTTTTTTTGGCCGGACAAGGGGTTTACGCCCCACCCG‑GCAAGGGGTTCCAACCCCCGAT‑GCGACCCtcggg                                                                                                                                                                                                      >  2:1037550/1‑235 (MQ=255)                                                  aTCCGGATACCGGTGCGCCGACTAAAGTATTGGCACCCGATACGGCAGGG‑AAATTTTACCCCAACCAGGATTGCAGTAGTTTGGGGCAGTAACCCGTCTTG‑AGACAGAAACAAACGCAAAACAGGCCAGAA‑GGATATATTTCAACAT‑TTTGAATTTGCACGTTTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCG‑GCAATGGTGCTCAACGCCTGa                                                                                                                                                                                                                    <  1:974146/220‑1 (MQ=255)                                                      ggATCCCGGTGCCCCGACTAAAGTTTTGGCACCCGATACGGCAGGG‑AAATTTTACCCCAACCAGGATTGCAGTAGTTTGGGGCAGTAACCCGTCTTG‑AGACAGAAACAAACGCAAAACAGGCCAGAA‑GGATATATTTCAACAT‑TTTGAATTTGCACGTTTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCG‑GCAATGGTGCTCAACGCCTGAT‑GCGACGCTGGCGCGTCTTATCa                                                                                                                                                                                            <  2:1086536/239‑1 (MQ=255)                                                       gttACCGGTGCCCCGAATAAAGTATTGGCACCCGATACGGCAGGG‑AAATTTTACCCCAACCAGGATTGCAGTAGTTTGGGGCAGTAACCCGTCTTG‑AGACAGAAACAAACGCAAAACAGGCCAGAA‑GGATATATTTCAACAT‑TTTGAATTTGCACGTTTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCG‑GCAATGGTGCTCAACGCCTGAT‑GCGACGCTGGCGCGTCTTATCAt                                                                                                                                                                                           <  2:916828/237‑1 (MQ=255)                                                                 gcccGCCTAAGGTTTTGTCCCCCGATTCGGCATGG‑AAATTTTACCCCAACCGGGATTGCAGTAGTTTGGGGCATTAACCCGTTTTG‑AGACCGAAACAAACGCAAACCAGGCCAGAA‑GGATATATTTCAACAT‑TTTGAATTTGCACGTTTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCG‑GCAATGGTGCTCAACGCCTGAT‑GCGACGCTGGCGCGTCTTATCATGCCT‑ACA‑Acc                                                                                                                                                                               <  2:818762/237‑1 (MQ=38)                                                                 cgcCGACTAAAGGATTGGCACCCGGTACGGCAGGG‑AAATTTTACCCCAACCAGGATTGCAGTAGTTTGGGGCAGTAACCCGTCTTG‑AGACAGAAACAAACGCAAAACAGGCCAGAA‑GGATATATTTCAACAT‑TTTGAATTTGCACGTTTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCG‑GCAATGGTGCTCAACGCCTGAT‑GCGACGCTGGCGCGTCTTATCATGCCT‑ACA‑Ac                                                                                                                                                                                <  1:41400/238‑1 (MQ=255)                                                                  gcCGACTAAAGTATTGGCACCCGATACGGCAGGG‑AAATTTTACCCCAACCAGGATTGCAGTAGTTTGGGGCAGTAACCCGTCTTG‑AGACAGAAACAAACGCAAAACAGGCCAGAA‑GGATATATTTCAACAT‑TTTGAATTTGCACGTTTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCG‑GCAATGGTGCTCAACGCCTGAT‑GCGACGCTGGCGCGTCTTATCATGCCT‑ACA‑Acc                                                                                                                                                                               <  1:372523/238‑1 (MQ=255)                                                                  cccaaCTAAAGTTTTGGCCCCCGGTACGGCAGGG‑AAATTTTACCCCAACCAGGATTGCAGTAGTTTGGGGCAGTAACCCGTCTTG‑AGACAGAAACAAACGCAAAACAGGCCAGAA‑GGATATATTTCAACAT‑TTTGAATTTGCACGTTTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCG‑GCAATGGTGCTCAACGCCTGAT‑GCGACGCTGGCGCGTCTTATCATGCCT‑ACA‑Acc                                                                                                                                                                               <  2:87676/234‑1 (MQ=255)                                                                    cGACTAAAGTATTGGCACCCGATACGGCAGGG‑AAATTTTACCCCAACCAGGATTGCAGTAGTTTGGGGCAGTAACCCGTCTTG‑AGACAGAAACAAACGCAAAACAGGCCAGAA‑GGATATATTTCAACAT‑TTTGAATTTGCACGTTTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCG‑GCAATGGTGCTCAAAGCCTGAT‑GCGACGCTGGCGCGCCTTATCATGCCT‑ACA‑Acccc                                                                                                                                                                             >  1:1016878/1‑238 (MQ=255)                                                                       cTAAAGGTTGGGCACCCGATTCGGCAGGG‑AAATTTTCCCCCAACCAGGATTGCCGTAGTTGGGGGCAGTAACCCGTCTTG‑AGACAGAAACAAACGCAAAACAGGCCAGAA‑GGATATATTTCAACAT‑TTTGAATTTGCACGTTTTTTGTAGGCCGGATAAGGCGTTTACCCCGCATCCG‑GCAATGGTGCTCAACGCCTGAT‑GCGACGCTGGCGCGTCTTATCATGCCT‑ACA‑ACCCCCCTc                                                                                                                                                                         <  2:946254/239‑1 (MQ=39)                                                                        tAAAGTTTTGGCACCCGATACGGCAGGG‑AAATTTTCCCCCAACCAGGATTGCAGTAGTTTGGGGCAGTAACCCGTCTTG‑GGACAGAAACAAACGCAAAACAGGCCAGAA‑GGATATATTTCAACAT‑TTTGAATTTGCACGTTTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCG‑GCAATGGTGCTCAACGCCTGAT‑GCGACGCTGGCGCGTCTTATCATGCCT‑ACA‑ACCCCCCTCa                                                                                                                                                                        <  2:7698/239‑1 (MQ=255)                                                                        tAAAGTATTGGCACCCGATACGGCAGGG‑AAATTTTACCCCAACCAGGATTGCAGTAGTTTGGGGCAGTAACCCGTCTTG‑AGACAGAAACAAACGCAAAACAGGCCAGAA‑GGATATATTTCAACAT‑TTTGAATTTGCACGTTTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCG‑GCAATGGTGCTCAACGCCTGAT‑GCGACGCTGGCGCGTCTTATCATGCCT‑ACA‑ACCCCCCTCa                                                                                                                                                                        <  1:644113/239‑1 (MQ=255)                                                                        tAAAGGATTGGCACCCGGTTCGGCAGGG‑AAATTTTACCCCAACCAGGATTGCAGTAGTTTGGGGCAGTAACCCGTCTTG‑AGACAGAAACAAACGCAAAACAGGCCAGAA‑GGATATATTTCAACAT‑TTTGAATTTGCACGTTTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCG‑GCAATGGTGCTCAACGCCTGAT‑GCGACGCTGGCGCGTCTTATCATGCCT‑ACA‑ACCCCCCTc                                                                                                                                                                         <  1:137742/238‑1 (MQ=255)                                                                           aGTATTGGCACCCGATACGGCAGGG‑AAATTTTACCCCAACCAGGATTGCAGTAGTTTGGGGCAGTAACCCGTCTTG‑AGACAGAAACAAACGCAAAACAGGCCAGAA‑GGATATATTTCAACAT‑TTTGAATTTGCACGTTTTTTTTAGGCCGGATAAGGCGTTTACGCCGCATCCG‑GCAATGGGGCTCAACGCCCGAT‑GCGACGGTGGGGGGGCTTCTAATGCCC‑AAACAcccccccaca                                                                                                                                                                       >  2:921116/1‑234 (MQ=255)                                                                                tGGCACCCGATACGGCAGGG‑AAATTTTACCCCAACCAGGATTGCAGTAGTTTGGGGCAGTAACCCGTCTTG‑AGACAGAAACAAACGCAAAACAGGCCAGAA‑GGATATATTTCAACAT‑TTTGAATTTGCACGTTTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCG‑GCAATGGTGCTCAACGCCTGAT‑GCGACGCTGGCGCGTCTTATCATGCCT‑ACa‑aac                                                                                                                                                                               >  1:878311/1‑222 (MQ=255)                                                                                tGGCACCCGATACGGCAGGG‑AAATTTTACCCCAACCAGGATTGCAGTAGTTTGGGGCAGTAACCCGTCTTG‑AGACAGAAACAAACGCAAAACAGGCCAGAA‑GGATATATTTCAACAT‑TTTGAATTTGCACGTTTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCG‑GCAATGGTGCTCAACGCCTGAT‑GCGACGCTGGCGCGTCTTATCATGCCT‑ACA‑Acc                                                                                                                                                                               <  2:878311/224‑1 (MQ=255)                                                                                tGGCACCCGATACGGCAGGG‑AAATTTTACCCCAACCAGGATTGCAGTAGTTTGGGGCAGTAACCCGTCTTG‑AGACAGAAACAAACGCAAAACAGGCCAGAA‑GGATATATTTCAACAT‑TTTGAATTTGCACGTTTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCG‑GCAATGGTGCTCAAACCCTGAT‑GCGACGCTGGCGCGGCTTATCATGCCT‑CCC‑ACCCCCCCCAAACCTTaa                                                                                                                                                                >  1:586214/1‑239 (MQ=255)                                                                                tGGCACCCGATACGGCAGGG‑AAATTTTACCCCAACCAGGATTGCAGTAGTTTGGGGCAGTAACCCGTCTTG‑AGACAGAAACAAACGCAAAACAGGCCAGAA‑GGATATATTTCAACAT‑TTTGAATTTGCACGTTTTTTGTAGGCCGGATAAAGCGTTTACGCCGCATCCG‑GCAATGGTGCTCAACGCCCGAT‑GCGACGCTGGCGCGTCTTATCATGCCT‑ACA‑ACCCCCCTCATACCTTaa                                                                                                                                                                >  1:163646/1‑239 (MQ=255)                                                                                tGGCACCCGATACGGCAGGG‑AAATTTTACCCCAACCAGGATTGCAGTAGGTTGGGGCAGGAAACCGTCTTG‑AGACAGAAACAAACGCAAAACAGGCCCGAA‑GGGTATATTTCAACAT‑TTTGAATTTGCACGTTTTTTGTAGGCCGGATAAAGCGGTTTCGCCCCAACCG‑GGAAAAGGGCTCAAACCCCGATGGGGGCGC‑GGCGCGGCTTTTCCTTCCT‑CCA‑Aaccccccccacccctaa                                                                                                                                                                >  2:685496/1‑228 (MQ=255)                                                                                    aCCCGATACGGCAGGG‑AAATTTTACCCCAACCAGGATTGCAGTAGTTTGGGGCAGTAACCCGTCTTG‑AGACAGAAACAAACGCAAAACAGGCCAGAA‑GGATATATTTCAACAT‑TTTGAATTTGCACGTTTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCG‑GCAATGGGGCTCAACCCCCGAT‑GCGGCGCGGGGCCGGCTTTTCATGCCTCAAA‑CCCCCCCCCACACCTTAGGGc                                                                                                                                                             >  2:828043/1‑239 (MQ=255)                                                                                       cGATGCGGCAGGG‑AAATTTTACCCCAACCAGGATTGCAGTTGTTTGGGGCATTAACCCGTCTTG‑AGACAGAAACAAACGCAAAACAGGCCAGAA‑GGATATATTTCAACAT‑TTTGAATTTGCACGTTTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCG‑GCAATGGTGCTCAACGCCTGAT‑GCGACGCTGGCGCGTCTTATCATGCCT‑ACA‑ACCCCCCTCATACCTTAAGGCGGAt                                                                                                                                                         <  1:1020974/239‑1 (MQ=255)                                                                                       cGATACGGCAGGG‑AAATTTTACCCCAACCAGGATTGCAATAGTTTGGGGCAGTAAACCGTCTTG‑AAACAGAAACAAACGACAAACAGGCCAGGA‑GGATATATTTCAACAT‑TTTGAATTTGCACGTTTTTTGTAGGCCGGCTAAAGCGTTTACGCCGCCACCG‑GCAATGGGGGCCAACCCCCGAT‑GCGGCGCTGGCGCGGCTTACACTACCT‑TCA‑ACCCCCCCCCTACCTTAcggcggga                                                                                                                                                         >  2:221360/1‑237 (MQ=255)                                                                                       cGATACGGCAGGG‑AAATTTTACCCCAAACAGGATTGCATTAGTTTGGGGCAGTAACCCGTCTGG‑AGACAGAAACAAACGCAAAACAGGCCAGAA‑GGATATATTTCAACAT‑TTTGAATTTGCACGTTTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCG‑TCAATGGTGCTCAACGCCTGAT‑GCGACGCTGGCGCGTCTTATCATGCCT‑ACA‑ACCCCCCTCATACCTTAAGGCGGAt                                                                                                                                                         <  1:129787/239‑1 (MQ=255)                                                                                       cGATACGGCAGGG‑AAATTTTACCCCAAACAGGATTGCAGTAGTTAGGGGCAGTAACCCGGCTTG‑AGACAGAAACAAACGCAAAACAGGCCAGAA‑GGATATATTTCAACAT‑TTTGAATTTGCACGTTTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCG‑GCAATGGTGCTCAACGCCTGAT‑GCGACGCTGGCGCGTCTTATCATGCCT‑ACA‑ACCCCCCTCATACCTTAAGGCGGAt                                                                                                                                                         <  1:499533/239‑1 (MQ=255)                                                                                       cGATACGGCAGGG‑AAATTTGACCCCAACCAGGATTGCAGTAGTTTGGGGCAGTAACCCGTCTTG‑AGACAGAAACAAACGCAAAACAGGCCAGAA‑GGATATATTTCAACAT‑TTTGAATTTGCACGTTTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCG‑GCAATGGTGCTCAACGCCTGAT‑GCGACGCTGGCGCGTCTTATCATGCCT‑ACA‑ACCCCCCTCATACCTTAAGGCGGAt                                                                                                                                                         <  1:48609/239‑1 (MQ=255)                                                                                        gATACGGCAGGG‑AAATTTTTCCCCAACCAGGATTGCAGTAGGTTGGGGCAGTAACCCGTCTTG‑AGACAGAAACAAACGCAAAACAGGCCAGAA‑GGATATATTTCATCAT‑TTTGAATTTGCACGTTTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCG‑GCAATGGTGCTCAACGCCTGAT‑GCGACGCTGGCGCGTCTTATCATGCCT‑ACA‑ACCCCCCTCATACCTTAAGGCGGATa                                                                                                                                                        <  1:651695/239‑1 (MQ=255)                                                                                                aGGG‑AAATTTTGCCCCAACCAGGGTTGCAGTAATTTGGGGCAGAAACCCATCTTG‑AGACAGAAACAAACGCAAAACAGGCCAGAA‑GGATATATTTCAACAT‑TTTGAATTTGCACGTTTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCG‑GCAATGGTGCTCAACGCCTGAT‑GCGACGCTGGCGCGTCTTATCATGCCT‑ACA‑ACCCCCCTCATACCTTAAGGCGGATAAGGCAtt                                                                                                                                                 <  1:579225/238‑1 (MQ=255)                                                                                                  gg‑AAATTTTACCCCAACCAGGATTGCAGTAGGTTGGGGCAGTAACCCGTCTTG‑AGACAGAAACAAACGCAAAACAGGCCAGAA‑GGATATATTTCAACAT‑TTTGAATTTGCACGTTTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCG‑GCAATGGGGCTCAACGCCTGAT‑GCGACGCTGGGGGGGCTTATCATGCCT‑ACA‑AACCCCCCCCAACCTTAAGGCGGGAAAGGAAttt                                                                                                                                                >  1:765187/1‑237 (MQ=38)                                                                                                      aaTTTTACCCCAACCAGGATTGCAGTAGTTTGGGGCAGTAACCCGTCTTG‑AGACAGAAACAAACGCAAAACAGGCCAGAA‑GGATATATTTCAACAT‑TTTGAATTTGCACGTTTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCG‑GCAATGGTGCTCAACGCCTGAT‑GCGACGCTGGCGCGTCTTATCATGCCT‑ACA‑ACCCCCCTCATACCTTAAGGCGGATAAGGCATTTACttt                                                                                                                                           <  1:997053/239‑1 (MQ=255)                                                                                                        ttttACCCCAACCAGGATTGCAGTAGTTTGGGGCAGTAACCCGTCTTG‑AGACAGAAACAAACGCAAAACAGGCCAGAA‑GGATATATTTCAACAT‑TTTGAATTTGCACGTTTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCG‑GCAATGGTGCTCAACGCCTGAT‑GCGACGCTGGCGCGTCTTATc                                                                                                                                                                                             <  1:686763/189‑1 (MQ=255)                                                                                                           tACCCCAACCAGGATTGCAGTAGTTTGGGGCAGTAACCCGTCTTG‑AGACAGAAACAAACGCAAAACAGGCCAGAA‑GGATATATTTCAACAT‑TTTGAATTTGCACGTTTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCG‑GCAATGGTGCTCAACGCCTGAT‑GCGACGCTGGCGCGTCTTATCATGCCT‑ACA‑ACCCCCCTCATACCTTAAGGCGGATAAGGCATTTACTTTATCAc                                                                                                                                      <  1:221360/239‑1 (MQ=255)                                                                                                           tACCCCAACCAGGATTGCAGTAGTTTGGGGCAGTAACCCGTCTTG‑AGACAGAAACAAACGCAAAACAGGCCAGAA‑GGATATATTTCAACAT‑TTTGAATTTGCACGTTTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCG‑GCAATGGTGCTCAACGCCTGAT‑GCGACGCTGGCGCGTCTTATCATGCCT‑ACA‑ACCCCCCTCATACCTTAAGGCGGATAAGGCATTTACTTTATCAc                                                                                                                                      <  1:828043/239‑1 (MQ=255)                                                                                                               caaaCCA‑GATTTCGGTAGTTTGGGGCAGTAACCCGGTTTGAAGAC‑GAAACAAACGCAAAAAATGCCAGAA‑TGATATAGTTCAACCT‑TTTGAATTTGCACGTTTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCG‑GCAATGGTGCTCAACGCCTGAT‑GCGACGCTGGCGCGTCTTATCATGCCT‑ACA‑ACCCCCCTCATACCTTAAGGCGGATAAGGCATTTACTTTAGCAC‑CGGGt                                                                                                                                <  2:842014/237‑1 (MQ=37)                                                                                                                  aCCAGGATTGCAGTAGTTTGGGGCAGTAACCCGTCTTG‑AGACAGAAACAAACGCAAAACAGGCCAGAA‑GGATATATTTCAACAT‑TTTGAATTTGCACGTTTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCG‑GCAATGGTGCTCAACGCCTGAT‑GCGACGCTTGCCCGTCTTCTCATGCCT‑CCa‑a                                                                                                                                                                                 >  2:686763/1‑189 (MQ=255)                                                                                                                  aCCAGGATTGCAGTAGTTTGGGGCAGTAACCCGTCTTG‑AGACAGAAACAAACGCAAAACAGGCCAGAA‑GGATATATTTCAACAT‑TTTGAATTTGCACGTTTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCG‑GCAATGGTGCTCAACGCCTGAT‑GCGACGCTGGCGCGTCTTATCATGCCT‑ACA‑ACCCCCCTCATACCTTAAGGCGGATAAGGCATTTACTTTATCAC‑CGGGTCa                                                                                                                              >  1:391617/1‑239 (MQ=255)                                                                                                                  aCCAGGATTGCAGTAGTTTGGGGCAGTAACCCGTCTTG‑AGACAGAAACAAACGCAAAACAGGCCAGAA‑GGATATATTTCAACAT‑TTTGAATTTGCACGTTTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCG‑GCAATGGTGCTCAACGCCTGAT‑GCGACGCTGGCGCGTCTTATCATGCCT‑ACA‑ACCCCCCTCATACCTTAAGGCGGATAAGGCATTTACTTTATCAC‑CGGGTCa                                                                                                                              <  1:764869/239‑1 (MQ=255)                                                                                                                  aCCAGGATTGCAGTAGTTTGGGGCAGTAACCCGTCTTG‑AGACAGAAACAAACGCAAAACAGGCCAGAA‑GGATATATTTCAACAT‑TTTGAATTTGCACGTTTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCG‑GCAATGGTGCTCAACGCCTGAT‑GCGACGCTGGCGCGTCTTATCATGCCT‑ACA‑ACCCCCCCCATACCTTAAGGCGGATAAGGCATTTACTTTATCCA‑CGGGCCa                                                                                                                              >  2:707594/1‑239 (MQ=255)                                                                                                                            cAGTAGTTTGGGGCAGTAACCCGTCTTG‑AGACAGAAACAAACGCAAAACAGGCCAGAA‑GGATATATTTCAACAT‑TTTGAATTTGCACGTTTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCG‑GCAATGGTGCTCAACGCCTGAT‑GCGACGCTGGCGCGTCTTATCATGCCT‑ACA‑ACCCCCCTCATACCTTAAGGCGGATa                                                                                                                                                        <  1:285638/204‑1 (MQ=255)                                                                                                                            cAGTAGTTTGGGGCAGTAACCCGTCTTG‑AGACAGAAACAAACGCAAAACAGGCCAGAA‑GGATATATTTCAACAT‑TTTGAATTTGCACGTTTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCG‑GCAATGGTGCTCAACGCCTGAT‑GCGACGCTGGCGCGTCTTATCATGCCT‑ACA‑ACCCCCCTCATACCTTAAGGCGGATAAGGCATTTACTTTATCAC‑CGGGTCAAACC‑AACaaa                                                                                                                   >  1:762997/1‑239 (MQ=255)                                                                                                                                 gtTTGGGGCAGTAACCCGTCTTG‑AGACAGAAACAAACGCAAAACAGGCCAGAA‑GGATATATTTCAACAT‑TTTGAATTTGCACGTTTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCG‑GCAATGGTGCTCAACGCCTGAT‑GCGACGCTGGCGCGTCTTATCATGCCT‑ACA‑ACCCCCCTCATACCTTAAGGCGGATAAGGCATTTACTTTATCAC‑CGGGTCAAACC‑AACAAAGCGa                                                                                                               >  1:212732/1‑238 (MQ=255)                                                                                                                                    tGGGGCAGTAACCCGTCTTG‑AGACAGAAACAAACGCAAAACAGGCCAGAA‑GGATATATTTCAACAT‑TTTGAATTTGCACGTTTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCG‑GCAATGGTGTTCAACGCCTGAT‑GCGACGCTGGCGCGCCTTATAATGCCT‑ACA‑ACCCCCCTCATACCTTAAGGCGGATAAAGCATTTACTTTATACC‑CGGGCCAAACA‑AAAAAAACCAccc                                                                                                            >  2:997746/1‑238 (MQ=38)                                                                                                                                    tGGGGCAGTAACCCGTCTTG‑AGACAGAAACAAACGCAAAACAGGCCAGAA‑GGATATATTTCAACAT‑TTTGAATTTGCACGTTTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCG‑GCAATGGTGCTCAACGCCTGTT‑GCGACGCTGGCGCGTCTTATCATGCCT‑ACA‑ACCCCCCCCATACCTTAAGGCGGCGAAGCCATTTACTTTA‑CACACGGGCCAAACCAAACAAAGAGAca                                                                                                             >  2:317752/1‑237 (MQ=38)                                                                                                                                      gggCAGTAACCCGTCTTG‑AGACAGAAACAAACGCAAAACAGGCCAGAA‑GGATATATTTCAACAT‑TTTGAATTTGCACGTTTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCG‑GCAATGGTGCTCAACGCCTGAT‑GCGACGCTGGCGCGGCTTATCATGCCT‑CCA‑ACCCCCCTCATACCTTAAGGCGGCTAAGGAAttttc                                                                                                                                              >  2:285638/1‑202 (MQ=255)                                                                                                                                       ggCCGTAACCCGTCTTG‑AGACAGAAAAAAAAGAAAAACAGGACAAAA‑GGTTATA‑TTCAAAATGTTTGAATGTGCACGTTTTTGGTAGGCCGGAAAAGGCGTTTACGCGGAATCGG‑GCAATGGTGCTCAACGCCGGAT‑GCGAGGCTGGCGCGTCTTATCAGGCAA‑ACA‑CCCCCCCTCATCCCTTAAGGCGGATAAGGCATTTACTTTATCAC‑CGGGTCAAACA‑AACAAAGCGACCCGcc                                                                                                         <  2:953608/238‑1 (MQ=255)                                                                                                                                           gTAACCCGTCTTG‑AGACAGAAACAAACGCAAAACAGGCCAGAA‑GGATATATTTCAACAT‑TTTGAATTTGCACGTTTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCG‑GCAATGGTGCTCAACGCCTGAT‑GCGACGCTGGCGCGTCTTATCATGCCT‑ACA‑ACCCCCCTCATACCTTAAg                                                                                                                                                               <  2:640379/182‑1 (MQ=255)                                                                                                                                           gTAACCCGTCTTG‑AGACAGAAACAAACGCAAAACAGGCCAGAA‑GGATATATTTCAACAT‑TTTGAATTTGCACGTTTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCG‑GCAATGGTGCTCAACGCCTGAT‑GCGACGCTGGCGCGTCTTATCATGCCT‑ACA‑ACCCCCCTCATACCTTAAg                                                                                                                                                               >  1:640379/1‑182 (MQ=255)                                                                                                                                            tAACCCGTCTTG‑AGACAGAAACAAACGCAAAACAGGCCAGTA‑GGATATATTTCAACAT‑TTTGAATTTGCACGTTTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCG‑GCAATGGTGCTCAACGCCTGTT‑GCGACGCTGGCGCGTCTTATCATGCCT‑ACA‑ACCCCCCTCCTACCTTAAGGCGGGTAAGGCATTTTATTTTTCAC‑CGGGCCAAACC‑AACAAAACAACCCCCCTTGata                                                                                                   >  2:899855/1‑239 (MQ=38)                                                                                                                                            tAACCCGTCTTG‑AGACAGAAACAAACGCAAAACAGGCCAGAA‑GGATATATTTCAACAT‑TTTGAATTTGCACGTTTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCG‑GCAATGGTGCTCAACGCCTGAT‑GCGACGCTGGCGCGTCTTATCATGCCT‑CCA‑ACCCCCCTCATACCTTAAGGCGGATAAGGCATTTACTTTATCAC‑CGGGTCAAACC‑AAAAAAGCGACCCCCCTTGaaa                                                                                                   >  2:1061242/1‑237 (MQ=255)                                                                                                                                            tAACCCGTCTTG‑AGACAGAAACAAACGCAAAACAGGCCAGAA‑GGATATATTTCAACAT‑TTTGAATTTGCACGTTTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCG‑GCAATGGTGCTCAACGCCTGAT‑GCGACGCTGGCGCGTCTTATCATGCCT‑ACA‑ACCCCCCTCa                                                                                                                                                                        >  1:877118/1‑172 (MQ=255)                                                                                                                                            tAACCCGTCTTG‑AGACAGAAACAAACGCAAAACAGGCCAGAA‑GGATATATTTCAACAT‑TTTGAATTTGCACGTTTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCG‑GCAATGGTGCTCAACGCCTGAT‑GCGACGCTGGCGCGTCTTATCATGCCT‑ACA‑ACCCCCCTCa                                                                                                                                                                        <  2:877118/172‑1 (MQ=255)                                                                                                                                            tAACCCGTCTTG‑AGACAGAAACAAACGCAAAACAGGCCAGAA‑GGATATATTTCAACAT‑TTTGAATTTGCACGTTTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCG‑GCAATGGTGCTCAACGCCTGAT‑GCGACGCTGGCGCGTCTTATCATGCCT‑ACA‑ACCCCCCTCATACCTTAAGGCGGATAAGGCATTTACTTTATCAC‑CGGGTCAAACC‑AACAAAGCGACCCGCCTTGata                                                                                                   >  1:719344/1‑239 (MQ=255)                                                                                                                                                            aaaaaacaaacCAAAAACAGGCCAAAA‑GGATAATTTTCAACAT‑TTTGAATTTGCACGTTTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCG‑GCAATGGTGCTCAACGCCTGAT‑GCGACGCTGGCGCGTCTTATCATGCCT‑ACA‑ACCCCCCTCATACCTTAAGGCGGATAAGGCATTTACTTTATCAC‑CGGGTCAAACC‑AACAAAGCGACCCGCCTTGATATAAATCCCAGGCTTc                                                                                    <  1:921116/236‑1 (MQ=255)                                                                                                                                                                aacaaacCTAAAGCAGG‑CAGACGGGATTTATTTAAACGT‑TTTGAATTTTCCGGTTTGTTTGAGGCGGGCTAGGTGTTTTACGCCCCATCCG‑GAAAGTGTGATCAACGACTTGT‑GCGACGCTGGCGCTTTTTACCATGCCT‑ACA‑ACCCCCTTCATCCCTTAAGGCGGATAGTGCATTTACTTTATCAC‑CGGGTCAAACC‑AACAAAGCGACCCGCCTTGATATAAATCCCAGGCTTCAGGt                                                                                <  2:615262/239‑1 (MQ=255)                                                                                                                                                                   aaccccAAAACGGGCCAGAA‑TTTTATTTTTTAAATT‑TTTGAATTGGCACGTTTTTTGTAGGCCGGATAGGGCGTTTACGCCGCATCCG‑GCAATGGTGCTCAACGCCTGAG‑GCGACGCTGGCGCGTTTTATCATGCCT‑ACA‑ACCCCCCTCATACCTTAAGGCGGATAAGGCATTTACTTTACCAC‑CGGGTCAAACC‑AACAAAGCGACCCGCCTTGATATAAATCCCAGGCTTCAGGTGcc                                                                             <  2:391617/234‑1 (MQ=255)                                                                                                                                                                      cacAAAACAGTCTGGAA‑GGATTTTTTTGAACAT‑TTTTAATTGGCATGTTTGTTGTGGGCCGGATAGGGCGTTTCCGCCGCTGCCG‑ACAATGGTGCTCACCGCCGGAT‑GCGACGGGGGCGCGTCTTATCTTGCCT‑ACA‑CCCCCCCTCATCCCTTAAGGCGGATAAGGCATTTACTTTATCAC‑CGGGTCAAACC‑AACAAAGCGCCCCGCCTTGATATAAATCCCAGGCTTCAGGTGCCCaa                                                                          <  2:585703/237‑1 (MQ=255)                                                                                                                                                                        cAAAACAGGCCAGAA‑GGATTAATTTCACCTT‑TTTGAATTGTCACGTTTTTTGAAGGCCGGATAAGGCGTTTACGCCGCATCCG‑GCAATGGTGCTCAACGCCTGAT‑GCGACGCTGGCGCGTTTTATCATGCCT‑ACA‑ACCCCCCTCATACCTTAAGGCGGATAAGGCATTTACTTTATCAC‑CGGGTCAAACC‑AACAAAGCGACCCGCCTTGATATAAATCCCAGGCTTCAGGTGCCCAACa                                                                        <  1:899855/239‑1 (MQ=39)                                                                                                                                                                                 ccAGAA‑GGATATATTTCAACAT‑TTTGAATTTGCACGTTTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCG‑GCAATGGTGCTCAACGCCTGAT‑GCGACGCTGGCGCGTCTTATCATGCCT‑ACA‑ACCCCCCTCATACCTTAAGGCGGATAAGGCATTTACTTTATCAC‑CGGGTCAAACC‑ACCAAAGCGCCCCGCCTTGATATAAATCCCAGGCTTCAGGTCCCCAACATAcgtaa                                                                 >  1:283182/1‑233 (MQ=255)                                                                                                                                                                                     aa‑GGATATATTTCAACAT‑TTTGAATTTGCACGTTTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCG‑GCAATGGTGCTCAACGCCTGAT‑GCGACGCTGGCGCGTCTTATCATGCCT‑ACA‑ACCCCCCTCATACCTTCAGGCGGGTAAGGCATTTACTTTATCAC‑CGGGCCAAACC‑ACCAAAGCGACCCGCCTTGTTATAAATCCCAGGCTTCAGGTGCCCAACAAAcggaaaatccg                                                           >  2:702945/1‑229 (MQ=38)                                                                                                                                                                                          ttatatTTCAACAT‑TGTGAATTTGCACGTTTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCG‑GCAATGGTGCTCAACGCCTGAT‑GCGACGCTGGCGCGTCTTATCATGCCT‑ACA‑CCCCCCCTCATACCTTAAGGCGGATAAGGCATTTACTTTATCAC‑CGGGTCAAACC‑AACAAAGCGACCCGCCTTGATATAAATCCCAGGCTTCAGGTGCCCAACATACTGTAACTGCGttt                                                        <  1:707594/237‑1 (MQ=255)                                                                                                                                                                                          atatatTTCAACAT‑TTTGAATTTGCACGTTGTTTGGAGGCCGGATAAGGCGTTTCCGCCGCATCCG‑GCAATGGTGCTCAACGCCTGAT‑GCGACGCTGGCGCGTCTTATCATGCCT‑ACA‑ACCCCCCTCATACCTTAAGGCGGATAAGGCATTTACTTTATCAC‑CGGGTCAAACC‑AACAAAGCGACCCGCCTTGATATAAATCCCAGGCTTCAGGTGCCCAACATACTGTAACTGCGttt                                                        <  1:702945/238‑1 (MQ=255)                                                                                                                                                                                           tatatTTCAACAT‑TTTGAATTTGCACGTTTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCG‑GCAATGGTGCTCAACGCCTGAT‑GCGACGCTGGCGCGTCTTATCATGCCT‑ACA‑ACCCCCCTCATACCTTAAGGCGGATAAGGCATTTACTTTATCAC‑CGGGTCAAACC‑AACAAAGCGACCCGCCTTGATATAAATCCCAGGCTTCAGGTGCCCAACATACTGTAACTGCGtt                                                         >  1:773532/1‑236 (MQ=255)                                                                                                                                                                                           tatatTTCAACAT‑TTTGAATTTGCACGTTTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCG‑GCAATGGTGCTCAACGCCTGAT‑GCGACGCTGGCGCGTCTTATCATGCCT‑ACA‑ACCCCCCTCATACCTTAAGGCGGATAAGGCATTTACTTTATCAC‑CGGGTCAAACC‑AACAAAGCGACCCCCCTTGATATAAATCCCAGGCTTCAGGTGCCCAAAATACTGAAACCGGGttttg                                                      >  2:556681/1‑237 (MQ=255)                                                                                                                                                                                           tatatTTCAACAT‑TTTGAATTTGCACGTTTTTTGTAGGCCGGATAAGGCGTTTACCCCGCATCCG‑GCAATGGTGCTCAACGCCTGAT‑GCGACGCTGGCGCGTCTTATCATGCCT‑ACA‑ACCCCCCTCATACCTTAAGGCGGATAAGGCATTTACTTTATCAC‑CGGGt                                                                                                                                <  2:55474/166‑1 (MQ=255)                                                                                                                                                                                           tatatTTCAACAT‑TTTGAATTTGCACGTTTTTTGTAGGCCGGATAAGGCGTTTACCCCGCATCCG‑GCAATGGTGCTCAACGCCTGAT‑GCGACGCTGGCGCGTCTTATCATGCCT‑ACA‑ACCCCCCTCATACCTTAAGGCGGATAAGGCATTTACTTTATCAC‑CGGGt                                                                                                                                >  1:55474/1‑166 (MQ=255)                                                                                                                                                                                                ttCAACAT‑TTTGAATTTGCACGTTTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCG‑GCAATGGTGCTCAACGCCTGAT‑GCGACGCTGGCGCGTCTTATCATGCCT‑ACA‑ACCACCCTCATACCTTAAGGCGGATAAGGCATTTACTTTATCAC‑CGGGTCAAACC‑AACAAAGCGACCCGCCGTGATATAAAACCCAGCATTCAGGGGCCCAACAAACTGGAACTGCGTTTCTGGCtt                                                 >  2:274666/1‑239 (MQ=38)                                                                                                                                                                                                 ccaccAT‑TTTGAATTTGCCCGTTTTTGTGAGGCCGGAAAGGGCGTTTCCGCCCCATCCG‑GAAATGGGCCACAACCCCTGAT‑GCGACGCTGGCGCGTCTTACCATGCCT‑ACA‑ACCCCCCTAATACCTTAAGGCGGATAAGGCATTTACTTTATCAC‑CGGGTCAAACC‑AACAAAGCGACCCGCCTTGATATAAATCCCAGGCTTCAGGTGCCCAACATACTGTAACTGCGTTTCTGGCTTa                                                <  1:1061242/235‑1 (MQ=255)                                                                                                                                                                                                   aaCAT‑TTTGAATTTGCACGTTTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCG‑GCAATGGTGCTCAACGCCTGAT‑GCGACGCTGGCGCGTCTTATCATGCCT‑ACA‑ACCCCCCTCATACCTTAAGGCGGATAAGGCATTTACTTTATCAC‑CGGGTCAAACC‑AACAAAGCGACCCGCCTTGATATAAATCCCAGGCTTCAGGGGCCCAACATACTGTAACTGCGTTTCTGGCttca                                               >  1:475205/1‑236 (MQ=255)                                                                                                                                                                                                     cAT‑TTTGAATTTGCACGTTTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCG‑GCAATGGTGCTCAACGCCTGAT‑GCGACGCTGGCGCGTCTTATCATGCCT‑ACA‑ACCCCCCTCATACCTTAAGGCGGATAAGGCATTTACTTTATCAC‑CGGGTCAAACC‑AACAAAGCGACCCGCCTTGATATAAATCCCAGGCTTCAGGTGCCCAAACAACTGTAAATTCGTTTCTGGCTTTAAc                                             >  1:354368/1‑238 (MQ=255)                                                                                                                                                                                                         tttGAATTTGCACGTTTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCG‑GCAATGGTGCTCAACGCCTGAT‑GCGACGCTGGCGCGTCTTATCATGCCT‑ACA‑ACCCCCCTCATACCTTAAGGCGGATAAGGCATTTACTTTATCAC‑CGGGTCAAACC‑AACAAAGCGACCCGCCTTGATCTAAATCCCAGGCTTCAGGGCCCCAACAAACTTGAACTCGGTTTTTGGCTTAAACGCaa                                         >  2:610082/1‑238 (MQ=38)                                                                                                                                                                                                          tgtaaTTTGCCTGTTTTTTGTAGGCCGAATAAGGGGTTTCCGCCGCATCCG‑GCAAGGGGCCTCAACGCCTGAT‑GCGACGCTGGGGGGTCTTATCATGCCT‑ACA‑ACCCCCCTCATACCTTAAGGCGGATAAGGCATTTACTTTATCAC‑CGGGTCAAACC‑AACAAAGCGACCCGCCTTGATATAAATCCCAGGCTTCAGGTGCCCAACATACTGTAACTGCGTTTCTGGCTTAAACGCaga                                        <  2:719344/236‑1 (MQ=255)                                                                                                                                                                                                                  gCACGTTTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCG‑GCAATGGTGCTCAACGCCTGAT‑GCGACGCTGGCGCGTCTTATCATGCCT‑ACA‑ACCCCCCTCATACCTTAAGGCGGATAAGGCATTTACTTTATCAC‑CGGGTCAAACC‑AACAAAGCGACCCGCCTTGATATAAATCCCAGGCTTCAGGTGCCCAACATACTGTAACTGCGTTTCTGGCTTAAACGCAGAGACATCAc                                >  1:880917/1‑239 (MQ=255)                                                                                                                                                                                                                     cGTTTTTTGTAGGCCGGATAAGGCGGTTACGCCGCATCCG‑GCAATGGTGCTCAACGCCTGAT‑GCGACGCTGGCGCGTCTTATCATGCCT‑ACA‑ACCCCCCTCATCCCTTAAGGCGGATAAGGCATTTACTTTATCAC‑CGGGTCAAACC‑AACAAAGCGACCCGCCTTGATATAAATCCCAGGCTTCAGGTGCCCAACATACTGTAACTGCGTTTCTGGCTTAAACGCAGAGACATCACCaa                             <  1:685496/239‑1 (MQ=255)                                                                                                                                                                                                                       ttttttGGTGGCCGGAATAGGGGTTTCCCCCGTATCCC‑GAAATGGTCCTCAACCCCTGAT‑GCGACGCGTGCGGGTCTTATCATGCCT‑ACA‑ACCCCCCCCATACCTTAAGGCGGAAAAGGCATTTACTTTATCAC‑CGGGTCAAACC‑AACAAAGCGACCCGCCTTGATATAAATCCCAGGCTTCAGGTGCCCAACATACTGTAACTGCGTTTCTGGCTTAAACGCAGAGACATCACCAATa                           <  2:475205/239‑1 (MQ=255)                                                                                                                                                                                                                       ttttttGGTGGCCGGA‑AAGGGGTTTTCCCCCCCACCGCGCAATGGGGCCCACCCCCTGAT‑GGGGCTCTGGCGCGTATTATCATGCCT‑ACA‑CCCCCCCTCATACCTTAAGGCGGATAAGGCATTTACTTTATCAC‑CGGGTCAAACC‑AACAAAGCGACCCGCCTTGATATAAATCCCAGGCTTCAGGTGCCCAACATACTGTAACTGCGTTTCTGGCTTAAACGCAGAGACATCACCAATa                           <  2:762997/239‑1 (MQ=255)                                                                                                                                                                                                                                ggCCGGAAAGGGTGTTTCCGCCCTACCCG‑GCAATGGTGCCCAACCCCTGAT‑GCGCCGCTGGCCGGTCTTATCATCCCT‑ACA‑ACCCCCCTCATCCCTTAAGGGGGATAAGGCATTTACTTTATCAC‑CGGGTCAAACC‑AACAAAGCGACCCGCCTTGATATAAATCCCAGGCTTCAGGTGCCCAACATACTGTAACTGCGTTTCTGGCTTAAACGCAGAGACATCACCAATACGCATATGc                  <  2:212732/239‑1 (MQ=255)                                                                                                                                                                                                                                 gCCGGATAAGGCGTTTACGCCGCATCCG‑GCAATGGTGCTCAACGCCTGAT‑GCGACGCTGGCGCGTCTTATCATGCCT‑ACA‑ACCCCCCTCATACCTTAAGGCGGATAAGGCATTTACTTTATCAC‑CGGGTCAAACC‑AACAAAGCGACCCGCCTTGATATAAATCCCAGGCTTCAGGTGCCCAACCTACTGTACCTGCGTTTCTGGCTTAAACGCAGAGCACTACCCAATACGCaaatcga                 >  1:510492/1‑236 (MQ=39)                                                                                                                                                                                                                                    ggATAAGGCGTTTACGCCGCATCCG‑GCAATGGTGCTCAACGCCTGAT‑GCGACGCTGGCGCGTCTTATCATGCCT‑ACA‑ACCCCCCTCATACCTTAAGGCGGATAAGGCATTTACTTTATCAC‑CGGGTCAAACC‑AACAAAGCGACCCGCCTTGATAAAAATcc                                                                                            <  2:712870/161‑1 (MQ=255)                                                                                                                                                                                                                                    ggATAAGGCGTTTACGCCGCATCCG‑GCAATGGTGCTCAACGCCTGAT‑GCGACGCTGGCGCGTCTTATCATGCCT‑ACA‑ACCCCCCTCATACCTTAAGGCGGATAAGGCATTTACTTTATCAC‑CGGGTCAAACC‑AACAAAGCGACCCGCCTTGATAAAAATcc                                                                                            >  1:712870/1‑161 (MQ=255)                                                                                                                                                                                                                                      aTAAGGCGTTTACGCCGCATCCG‑GCAATGGTGCTCAACGCCTGAT‑GCGACGCTGGCGCGTCTTATCATGCCT‑ACA‑ACCCCCCTCATACCTTAAGGCGGATAAGGCATTTACTTTATCAC‑CGGGTCAAACC‑AACAAAGCGACCCGCCTTGATATAAATCCCAGGCTTCAGGTGCCCAACATACCGTAACTGCGTTTCTGGCTTAAACGCAGAGCCATCACCAATACGCATATGCATCCGa            >  1:849471/1‑239 (MQ=255)                                                                                                                                                                                                                                      aTAAGGCGTTTACGCCGCATCCG‑CAAAGGGTGCCAAACGCCGTAG‑GCGACGCTGGCGCGTCTTATCATGCCT‑ACA‑ACCCCCCTCATACCTTAAGGCGGATAAGGCATTTACTTTATCCC‑CGGGTCAAACC‑AACAAAGCGCCCCGCCTTGATATAAATCCCAGGCTTCAGGTGCCCAACATACTGTAACTGCGTTTCTGGCTTAAACGCAGAGACATCACCAATACGCATATGCATCAg             <  1:610082/238‑1 (MQ=255)                                                                                                                                                                                                                                               ttACGCCGCATCCG‑GCAATGGTGCTCAACGCCTGAT‑GCGACGCTGGCGCGTCTTATCATGCCT‑ACA‑ACCCCCCTCATCCCTTAAGGCGGATAAGGCATTTACTTTATCAC‑CGGGTCAAACC‑AACAAAGCGACCCGCCTTGATATAAATcc                                                                                            <  1:594820/150‑1 (MQ=255)                                                                                                                                                                                                                                               ttACGCCGCATCCG‑GCAATGGTGCTCAACGCCTGAT‑GCGACGCTGGCGCGTCTTATCATGCCT‑ACA‑ACCCCCCTCATACCTTAAGGCGGATAAGGCATTTACTTTATCAC‑CGGGTCAAACC‑AACAAAGCGACCCGCCTTGATATAAATcc                                                                                            >  2:594820/1‑150 (MQ=255)                                                                                                                                                                                                                                               ttACGCCGCATCCG‑GCAATGGTGCTCAACGCCTGAT‑GCGACGCTGGCGCGTCTTATCATGCCT‑ACA‑ACCCCCCTCATACCTTAAGGCGGATAAGGCATTTACTTTATCAC‑CGGGTCAAACC‑AACAAAGCGACCCGCCTTGATATAAATCCCAGGCTTCAGGTGGCCAACATACTGTAACTGCGTTTCTGGCTTAAACGCAAAGACATCACCAAAAAGCAAATACATAAGAACGGCAggg   >  2:454203/1‑239 (MQ=255)                                                                                                                                                                                                                                                tACGCCGCATCCG‑GCAATGGTGCTCAACGCCTGAT‑GCGACGCTGGCGCGTCTTATCATGCCT‑ACA‑ACCCCCCTCATACCTTAAGGCGGATAAGGCATTTACTTTATCAC‑CGGGTCAAACC‑AACAAAGCGCCCCGCCTTGATATAAATCCCAGGCTTCAGGGGCCCAACATACTGTAACTGCGTTTCTGGCTTAAACGCAGAGACATCACCAATCAGCATATGCATAAGATCGGAAGGGc  >  2:8102/1‑239 (MQ=255)                                                                                                                                                                                                                                                tACGCCGCATCCG‑GCAATGGTGCTCAACGCCTGAT‑GCGACGCTGGCGCGTCTTATCATGCCT‑ACA‑ACCCCCCTCATACCTTAAGGCGGATAAGGCATTTACTTTATCAC‑CGGGTCAAACC‑AACAAAGCGACCCGCCTTGATATAAATCCCAGGCTTCAGGTGCCCAACATACTGTAACTGCGTTTCTGGCTTAACCGCAAAACCATCACCAATAAGCAAAAGCATCAAATCGGCCgggg  >  2:745783/1‑238 (MQ=255)                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                  GAAAAACCGGTGTTACTGTCAGTAGAAGGTCACTTTACGCTTGAGGGTAATCCGGATACCGGTGCGCCGACTAAAGTATTGGCACCCGATACGGCAGGG‑AAATTTTACCCCAACCAGGATTGCAGTAGTTTGGGGCAGTAACCCGTCTTG‑AGACAGAAACAAACGCAAAACAGGCCAGAA‑GGATATATTTCAACAT‑TTTGAATTTGCACGTTTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCG‑GCAATGGTGCTCAACGCCTGAT‑GCGACGCTGGCGCGTCTTATCATGCCT‑ACA‑ACCCCCCTCATACCTTAAGGCGGATAAGGCATTTACTTTATCAC‑CGGGTCAAACC‑AACAAAGCGACCCGCCTTGATATAAATCCCAGGCTTCAGGTGCCCAACATACTGTAACTGCGTTTCTGGCTTAAACGCAGAGACATCACCAATACGCATATGCATCAGATCGGCAGGGC  >  NC_000913/215839‑216312

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑