Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,511,428 G→C 18.3% intergenic (+101/+40) ypeC → / ← mntH DUF2502 family putative periplasmic protein/manganese/divalent cation transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,511,4280GC18.3% 108.4 / 3.5 53intergenic (+101/+40)ypeC/mntHDUF2502 family putative periplasmic protein/manganese/divalent cation transporter
Reads supporting (aligned to +/- strand):  ref base G (27/15);  new base C (4/7);  total (31/22)
Fisher's exact test for biased strand distribution p-value = 1.68e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTTGGTGGCGTCATGATAATGGCTACCACCGTGGCTGGGATAAGCGTAAAGCGTATGAGCGTGGCTATCGTGAAGGCTGGCGCGATCGTGACGATCATCGCGGAAAAGGCCGGGGTCATGGGCACCGCCATTAAACGCGTCCGCCAATGGAGCACAATGCCTGATGCGACGCTAGTGCGTCTTATCAGGCCTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGCACCACAATCACCCAGCCTGTCTGTTTTACGCGTTTGCTGTTCACCAGATCGCCCATCAACTTGCTGTCACTGGTGAAAATCAGCAGTGGAACCAGCGCCAGAGCGATACCAAAACTTAACAGTACCTGACTCATAAC  >  NC_000913/2511194‑2511659
                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                       
gTTGGTGGCGTCATGATAATGGCTACCACCGTGGCTGGGATAAGCGTAAAGCGTATGAGCGTGGCTATCGTGAAGGCTGGCGCGATCGTGACGATCATCGCGGAAAAGGCCGGGGTCATGGGCCCCGCCATTAAACGCGTCCGCCAATGGAGCACAATGCCTGTTGCGCCCCTGGTGCGTCTTATCAGGCCCACCAACCGGCCCTTTAACCATGAGGGGGGGACAGGGGTTTAcgcccc                                                                                                                                                                                                                                     >  2:1088431/1‑237 (MQ=255)
                                           gcgtaaagcgtaTGAGCGTGGCTATCGTGAAGGCTGGCGCGATCGTGACGATCATCGCGGAAAAGGCCGGGGTCATGGGCACCGCCATTAAACGCGTCCGCCAATGGAGCACAATGCCTGATGCGACGCTAGTGCGTCTTATCAGGCCTACAAACCGGCTCTTTAACGCGTAGGGCGGCTAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAACTACaaa                                                                                                                                                                                           >  1:463418/1‑237 (MQ=255)
                                               aaagcgtaTGAGCGTGGCTATCGTGAAGGCTGGCGCGATCGTGACGATCATCGCGGAAAAGGCCGGGGTCATGGGCACCGCCATTAAACGCGTCCGCCAATGGAGCACAATGCCTGATGCGACGCTAGTGCGTCTTATCAGGCCTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACCCCGAATCCGCCAATATGGCCAAATGGGACCCCAATTCACCAAAAACCCCa                                                                                                                                                                                       >  2:916877/1‑238 (MQ=255)
                                                aagcgtaTGAGCGTGGCTATCGTGAAGGCTGGCGCGATCGTGACGATCATCGCGGAAAAGGCCGGGGTCATGGGCACCGCCATTAAACGCGTCCGCCAATGGAGCACAATGCCTGATGCGACGCTAGTGCGTCTTATCAGGCTTCCAAACCGGCCCTTTAACGCGTAGGGCGGTTAAGGCGTTTACCCCGACTCACCCCATAATGCCAAATGAGACGCCCATTCAACTCAAACCCaaa                                                                                                                                                                                     >  2:483096/1‑236 (MQ=255)
                                                     taTGAGCGTGGCTATCGTGAAGGCTGGCGCGATCGTGACGATCATCGCGGAAAAGGCCGGGGTCATGGGCACCGCCATTAAACGCGTCCGCCAATGGAGCACAATGCCTGATGCGACGCTAGTGCGTCTTATCAGGCCTAAAACCCGGCTCTTTAACGCGTAGGGCGGTTCAGGCGTTTACCCCGCATCCGCCAATAATGCCAGATGGGACGCCAATCCCACTACAAACCCAGCCCCg                                                                                                                                                                                 >  2:284933/1‑238 (MQ=255)
                                                     taTGAGCGTGGCTATCGTGAAGCCTGGCGCGATCGTGACGATCATCGCGGAAAAGGCCGGGGTCATGGGCCCCGCCATTAAACGCGTCCGCCAATGGAGCACAATGCCTGATGCGACGCTAGTGCGTCTTATCAGGCCTACAAACCGGCTCTTTACCGCGTAGGGCGGCTAAGGGGTTTACCCCCCATCCGCCCCCAGTACCAGATACGACCCCCACTCAAATACAACCCCACCCCCg                                                                                                                                                                                 >  2:566754/1‑238 (MQ=255)
                                                                        aaGGCTGGCGCGATCGTGACGATCATCGCGGAAAAGGCCGGGGTCATGGGCACCGCCATTAAACGCGTCCGCCAATGGAGCACAATGCCTGATGCGACGCTAGTGCGTCTTATCAGGCCTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATACGACGCTCATTCAACTACAATCCCAGCGCCGTCCCCACCAACAACCAGata                                                                                                                                                             >  1:141089/1‑239 (MQ=255)
                                                                                     tCGTGACGATCATCGCGGAAAAGGCCGGGGTCATGGGCACCGCCATTAAACGCGTCCGCCAATGGAGCACAATGCCTGATGCGACGCTAGTGCGTCTTATCAGGCCTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCCCACCCACAACCAGACATTCAGCCCCACg                                                                                                                                                 >  1:420912/1‑238 (MQ=255)
                                                                                         gACGATCATCGCGGAAAAGGCCGGGGTCATGGGCACCGCCATTAAACGCGTCCGCCAATGGAGCACAATGCCTGATGCGACGCTAGTGCGTCTTATCAGGCCTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAAATGCGACGCCCATTCAACTACAATCCCAGCCCCCTCCCCCCCAAAAACCAGATATTCACCCCCCCAACCac                                                                                                                                            >  2:833389/1‑238 (MQ=255)
                                                                                               catcGCGGAAAAGGCCGGGGTCATGGGCACCGCCATTAAACGCGTCCGCCAATGGAGCACAATGCCTGATGCGACGCTAGTGCGTCTTATCAGGCCTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAACTACAATCCCACCGCCGTCCCCACCAACCACCAAGTATTCAGCGCCACGACCAGcaccac                                                                                                                                      >  1:67011/1‑239 (MQ=255)
                                                                                                 tcGCGGAAAAGGCCGGGGTCATGGGCACCGCCATTAAACGCGTCCGCCAATGGAGCACAATGCCTGATGCAACGCTAGTGCGTCTTATCAGGCCTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAACTCCCATCCCACCGCCGTCCCCCCCAAAAACCAGAAATTCCACCCCACCACCAGcacccaaa                                                                                                                                    >  2:412690/1‑235 (MQ=255)
                                                                                                                 ggTCATGGGCACCGCCATTAAACGCGTCCGCCAATGGAGCACAATGCCTGATGCGACGCTAGTGCGTCTTATCAGGCCTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCCCATTCAACTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCCCGACCAGCACCACAAACACCCAAcccg                                                                                                                        >  2:25156/1‑233 (MQ=255)
                                                                                                                     aTGGGCACCGCCATTAAACGCGTCCGCCAATGGAGCACAATGCCTGATGCGACGCTAGTGCGTCTTATCAGGCCTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCCCCCCAACAACCAGATATTCAGCCCCCCGCCCAGCCCCAAAAACACCCACCCTGACTGttt                                                                                                                 >  2:472046/1‑238 (MQ=255)
                                                                                                                      tGGGCACCGCCATTAAACGCGTCCGCCAATGGAGCACAATGCCTGATGCGACGCTAGTGCGTCTTATCAGGCCTACAAACCGGCTCTTTAGCGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAACTACAATCCCACCGCCGTCCCCCCCAACAACCCGCTATTCAACGCCACGACCAGCCCCCAAACCacccaccccgctcgtttt                                                                                                                >  2:84247/1‑228 (MQ=255)
                                                                                                                      tGGGCACCGCCATTAAACGCGTCCGCCAATGGAGCACAATGCCTGATGCGACGCTAGTGCGTCTTATCAGGCCTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGCACCACAATCACCCAGCCTGTCTGtttt                                                                                                                >  1:1033052/1‑238 (MQ=255)
                                                                                                                            ccGCCATTAAACGCGTCCGCCAATGGAGCACAATGCCTGATGCGACGCTAGTGCGTCTTATCAGGCCTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCCCCCCAACAACCAGATATTCAGCGCCACGACCAGAACCAAAATCACCCAACCCGTCTGTTTTACACGtt                                                                                                         >  2:471775/1‑239 (MQ=255)
                                                                                                                             cGCCCTTAAACCCTGCCCCCCATTGGGCACAATGCCCTGTGGCGACGATGGGGTGTTTTACCGGGCCTCAAAACCGGCTCTTTAACGCGTGGGGCGGATAAGGCGTTTCCGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGCACCACAATCACCCAGCCTGTCTGTTTTACGCGtt                                                                                                         <  2:530312/239‑1 (MQ=255)
                                                                                                                             cGCCATTAAACGCGTCCGCCAATGGAGCACAATGCCTGATGCGACGCTAGTGCGTCTTATCAGGCCTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGCCGCTCATTCAACTACAATCCCAGCGCCGCCCCCACCAACACCCAGATATTCAgcg                                                                                                                                                      >  1:236127/1‑193 (MQ=255)
                                                                                                                             cGCCATTAAACGCGTCCGCCAATGGAGCACAATGCCTGATGCGACGCTAGTGCGTCTTATCAGGCCTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCCCACCAACAACCAGTAATTCAGCCCCACGACCACCACCCCAACACCCCCCCCTGCCTGTTTTACGCGtt                                                                                                         >  2:933023/1‑238 (MQ=255)
                                                                                                                             cGCCATTAAACGCGTCCGCCAATGGAGCACAATGCCTGATGCGACGCTAGTGCGTCTTATCAGGCCTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGCACCCCAATCCCCAAGCCCGCCTGTTTTACGCGctt                                                                                                        >  1:530312/1‑239 (MQ=255)
                                                                                                                             cGCCATTAAACGCGTCCGCCAATGGAGCACAATGCCTGATGCGACGCTAGTGCGTCTTATCAGGCCTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAACTACAATCCCAGCCCCGTCCCCCCCAACAACCAGATATTCAGCGCCCCCCCCACCACCCCAATCACCCCCCCCTTCTTTTTTACGCGttt                                                                                                        >  2:553820/1‑239 (MQ=255)
                                                                                                                                aaTTAAACGCGTCCCCCAATGGGACAAAATGCCTGATGGGACGCGAGTGCGTCTTACCAGGCCTAAAAACCGGCTCTTTAACGCGTAGGGCGGAAAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGCACCACAATCACCCAGCCTGTCTGTTTTACGCGTTTGCt                                                                                                     <  1:42403/238‑1 (MQ=255)
                                                                                                                                   tAAACGGCTCCGCCAATGGAGCACAATGCCTGATGCGACGTTGGTGCGTCTTATCAGGCCTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGCACCACAATCACCCAGCCTGTCTGTTTTACGCGTTTGCTGtt                                                                                                  <  1:843012/239‑1 (MQ=255)
                                                                                                                                           cccGCAAATGGAGCACAATGCCTGATGCGACGCTAGTGCGTCTTACCAGGCCTAAAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGCACCACAATCACCCAGCCTGTCTGTTTTACGCGTTTGCTGTTCACCAGAt                                                                                          <  1:25156/238‑1 (MQ=255)
                                                                                                                                            ccGCCAATGGAGCACAATGCCTGATGCGACGCTAGGGCGTCTTATCAGGCCTATAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGGTTACGCCGAATCCGCCAATAGTGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGCACCACAATCACCCAGCCTGTCTGTTTTACGCGTTTGCTGTTCACCAGAt                                                                                          <  1:3342/238‑1 (MQ=255)
                                                                                                                                                 aaTGGAGCACAAGTCCTGGTGCGGCGGTAGGGGGTTTTATCAGGCCTACAAACCGGCTCTTTAACGGGTGGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGCACCACAATCACCCAGCCTGTCTGTTTTACGCGTTTGCTGTTCACCAGATCGCCCa                                                                                    <  1:277668/239‑1 (MQ=255)
                                                                                                                                                        cacaATGCCTGATGCGACGCTAGTGCGTCTTATCAGGCCTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCCCCCCAACAACCAGATATTCAGCGCCACGACCAGCACCACAATCACCCAGCCTGTCTGTTTTACGCGTTTGCTGTTCACCAGATCGCCCATCAACt                                                                              >  1:846392/1‑238 (MQ=255)
                                                                                                                                                          caATGCCTGATGCGACGCTAGTGCGTCTTATCAGGCCTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGCACCACAATCACCCAGCCTGTCTGTTTTACGCGTTTGCTGTTCACCAGATCGCCCATCAACtt                                                                             >  1:260223/1‑237 (MQ=255)
                                                                                                                                                                   gATGCGACGCTAGTGCGTCTTATCAGGCCTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGCACCACAATCACCCAGCCTGTCAGTTTTACGCGTTTGCTGTTCACCAGACCGCCAATCAACTTGCTGTAACTg                                                                   >  1:387861/1‑239 (MQ=255)
                                                                                                                                                                    aTGCGACGCTAGTGCGTTTTATCAGGCCTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACg                                                                                                                                                                                                           <  2:113842/102‑1 (MQ=255)
                                                                                                                                                                    aTGCGACGCTAGTGCGTCTTATCAGGCCTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACg                                                                                                                                                                                                           >  1:113842/1‑102 (MQ=255)
                                                                                                                                                                          cGCTAGTGCGTCTTATCAGGCCTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGCCCCACAATCACCCAGCCTGTCTGTTTTACGCGTTTGCTGTTCACCAAATCGCCCAACAACTTGCTGTAACTGGTGaaaa                                                            >  2:487868/1‑239 (MQ=255)
                                                                                                                                                                                    tCTTATCAGGCCTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGCACCACAATCACCCAGc                                                                                                                           >  1:789448/1‑166 (MQ=255)
                                                                                                                                                                                    tCTTATCAGGCCTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGCACCACAATCACCCAGCCTGTCTGTTTTACGCGTTTGCTTTTCCCCAGAACCCCCACCAACTTCCTGTCCCTGGTGAAAATcagaaggga                                                  >  2:669035/1‑236 (MQ=255)
                                                                                                                                                                                        catggccatACAAACCGGCTCTTTAACCGGTGGGGCGATAAAGGGGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGCACCACAATCACCCAGCCTGTCTGTTTTACGCGTTTGCTGTTCACCAGATCGCCCATCAACTTGCTGTCACTGGTGAAAATCAGCAGTGGAAcc                                              <  2:941859/231‑1 (MQ=255)
                                                                                                                                                                                              ccTACAAACCGGCTTTTTAACGGGTGGGGGGGAAAAGGCTTTTACGCCCCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAACTACAACCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGCACCACAATCACCCAGCCTGTCTGTTTTACGCGTTTGCTGTTCACCAGATCGCCCATCAACTTGCTGTCACTGGTGAAAATCAGCAGTGGAACCAGCGcc                                        <  2:1033052/239‑1 (MQ=255)
                                                                                                                                                                                                   aaaCCCGCCTTTTAACCGTAGGGGCGGATAAGGCTTTACCCCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGCACCACAATCACCCAGCCTGTCTGTTTTACGCGTTTGCTGTTCACCAGATCGCCCATCAACTTGCTGTCACTGGTGAAAATCAGCAGTGGAACCAGCGCCAGAGc                                   <  1:471775/239‑1 (MQ=255)
                                                                                                                                                                                                       cGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGCACCACAATCACCAAGCCTGTCTGTTTTACGCGTTTGCTGTTCACCAGATCGCCCATCAACTTGCTGTCACTGGTGTAAATCAGCATCGGAACAAGAGCCAGAGCGAt                                >  1:595618/1‑238 (MQ=255)
                                                                                                                                                                                                           tttttAACGCGGAGGGCGGATAGGGCTTTTACGCCCCTTCCGCCAATAGTGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGCACCACAATCACCCAGCCTGTCTGTTTTACGCGTTTGCTGTTCACCAGATCGCCCATCAACTTGCTGTCACTGGTGAAAATCAGCAGTGGAACCAGCGCCAGAGCGATACCa                            <  1:84247/236‑1 (MQ=255)
                                                                                                                                                                                                             tttAACCGGTAGGGGGGAAAAGCCGTTTACCCCCCACCCGCCAATAGTGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGCACCACAATCACCCAGCCTGTCTGTTTTACGCGTTTGCTGTTCACCAGATCGCCCATCAACTTGCTGTCACTGGTGAAAATCAGCAGTGGAACCAGCGCCAGAGCGATACCaaaa                         <  1:472046/239‑1 (MQ=255)
                                                                                                                                                                                                               tAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGCACCACAATCACCCAGCCTGTCTGTTTTACGCGTTTGCTGTTCACCAGATCGCCCATCAACTTGCTGTCACTGGTTAAAATCAGCAGTGGAACCAGCGCCAGAGCGTTACCacacct                       >  1:1069272/1‑234 (MQ=255)
                                                                                                                                                                                                                tacccagtgggggggATAAGGCTTTTACCCCGCATCCGCCAATAGTGCCGAATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCCCCCCAACAACCAGATATTCAGCGCCACGACCAGCACCACAATCACCCAGCCTGTCTGTTTTACGCGTTTGCTGTTCACCAGATCGCCCATCAACTTGCTGTCACTGGTGAAAATCAGCAGTGGAACCAGCGCCAGAGCGATACCAAAACt                       <  2:420912/230‑1 (MQ=255)
                                                                                                                                                                                                                  cgcgTAGGGCGGAAAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGCACCACAATCACCCAGCCTGTCTGTTTTACGCGTTTGCTGTTCACCAGATCGCCCATCAACTTGCTGTCACTGGTGAAAATCAGCAGTGGAACCAGCGCCAGAGCGATACCAAAACTTaa                    <  1:833389/239‑1 (MQ=255)
                                                                                                                                                                                                                   ccgtgggggCGGTTAGGGCTTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGCACCACAATCCCCCAGCCTGTCTGTTTTACGCGTTTGCTGTTCACCAGATCGCCCATCAACTTGCTGTCACTGGTGAAAATCAGCAGTGGAACCAGCGCCAGAGCGATACCAAAACTTaa                    <  2:1069272/233‑1 (MQ=255)
                                                                                                                                                                                                                              gATATGCCGTTCACCCCGCTTCCTCCAAGTGGTCCAGATGCGACGCTCACTCACCTACAATCCCAGCGCCCGCCCCACCAACAACCAGATATTCAGCGCCACGACCAGCACCACAATCACCCAGCCTGTCTGTTTTACGCGTTTGCTGTTCACCAGATCGCCCATCAACTTGCTGTCACTGGTGAAAATCAGCAGTGGAACCAGCGCCAGAGCGATACCAAAACTTAACAGTACCTGa          <  1:323845/238‑1 (MQ=255)
                                                                                                                                                                                                                               aTAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGCACCACAATCACCCAGCCTGTCTGTTTTACGCGTTTGCTGTTCACCAGATCGCCCATCAACTTGCTGTCACTGGTGAAAATCAGCAGTGGAACCAGCGCCAGAGCGATACCAAAACTTAACAGTACCTGACt        >  1:144849/1‑239 (MQ=255)
                                                                                                                                                                                                                               aTAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGCACCACAATCACCCAGCCTGTCTGTTTTACGCGTTTGCTGTTCACCAGATCGCCCATCAACTTGCTGTCACTGGTGAAAATCAGCAGTGGAACCAGCGCCAGAGCGATACCAAAAATTAACAATACCTGAc         >  1:120144/1‑238 (MQ=255)
                                                                                                                                                                                                                                 agggCTTTTACGCCCCACCCGCCAATAGTGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCCCCCCAACAACCAGATATTCAGCGCCACGACCAGCACCACAATCACCCAGCCTGTCTGTTTTACGCGTTTGCTGTTCACCAGATCGCCCATCAACTTGCTGTCACTGGTGAAAATCAGCAGTGGAACCAGCGCCAGAGCGATACCAAAACTGAACAGTACCTGACTCa      <  1:4587/237‑1 (MQ=255)
                                                                                                                                                                                                                                 aagggctttACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGCACCACAATCACCCAGCCTGTCTGTTTTACGCGTTTGCTGTTCACCAGATCGCCCATCAACTTGCTGTCACTGGTGAAAATCAGCAGTGGAACCAGCGCCAGAGCGATACCAAAACTTAACAGTACCTGACTCa      <  1:933023/233‑1 (MQ=255)
                                                                                                                                                                                                                                 aaGGCTTTTACCCCGCATCCGCAAATAGTCCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGCACCACAATCACCCAGCCTGTCTGTTTTACGCGTTTGCTGTTCACCAGATCGCCCATCAACTTGCTGTCACTGGTGAAAATCAGCAGTGGAACCAGCGCCAGAGCGATACCAAAACTTAACAGTACCTGACTCa      <  1:566754/239‑1 (MQ=255)
                                                                                                                                                                                                                                 aaGGCCTTTCCCCCACATCCGCCAATAGTGCCAGATGCGACCCTCATTCAACTACAACCCCAGCGCCGTCCCCCCCAACAACCAGATATTCAGCGCCACGACCAGCACCACAATCACCCAGCCTGTCTGTTTTACGCGTTTGCTGTTCACCAGATCGCCCATCAACTTGCTGTCACTGGTGAAAATCAGCAGTGGAACCAGCGCCAGAGCGATACCAAAACTTAACAGTACCTGACTCa      <  1:989271/239‑1 (MQ=255)
                                                                                                                                                                                                                                      gTTTACCCCGGATCCCCCAATAGTGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGCACCACAATCACCCAGCCTGGCTGTTTTACGCGTTTGCTGTTCACCAGATCGCCCATCAACTTGCTGTCACTGGTGAAAATCAGCAGTGGAACCAGCGCCAGAGCGATACCAAAACTTAACAGTACCTGACTCATAAc  <  1:101691/238‑1 (MQ=255)
                                                                                                                                                                                                                                         tACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGCACcaca                                                                                                                                     >  1:904233/1‑104 (MQ=255)
                                                                                                                                                                                                                                         tACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGCACcaca                                                                                                                                     <  2:904233/104‑1 (MQ=255)
                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                       
GTTGGTGGCGTCATGATAATGGCTACCACCGTGGCTGGGATAAGCGTAAAGCGTATGAGCGTGGCTATCGTGAAGGCTGGCGCGATCGTGACGATCATCGCGGAAAAGGCCGGGGTCATGGGCACCGCCATTAAACGCGTCCGCCAATGGAGCACAATGCCTGATGCGACGCTAGTGCGTCTTATCAGGCCTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGCACCACAATCACCCAGCCTGTCTGTTTTACGCGTTTGCTGTTCACCAGATCGCCCATCAACTTGCTGTCACTGGTGAAAATCAGCAGTGGAACCAGCGCCAGAGCGATACCAAAACTTAACAGTACCTGACTCATAAC  >  NC_000913/2511194‑2511659

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: