Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,999,610 A→T 15.4% R94R (CGT→CGA ygeR ← LysM domain‑containing M23 family putative peptidase; septation lipoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,999,6100AT15.4% 191.1 / 3.6 78R94R (CGT→CGAygeRLysM domain‑containing M23 family putative peptidase; septation lipoprotein
Reads supporting (aligned to +/- strand):  ref base A (26/38);  new base T (11/2);  total (37/41)
Fisher's exact test for biased strand distribution p-value = 5.24e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTTGCCCACATACACCACCTTTCCTGCACCCGCGGCGTAAATAGGTGTACCCCGTGGAGCTGAGATATCAATCCCTTTATTGCCGCCATCTGCTGTCGAATACGGCATGATAACTTTCCCTGTCGTTGGCCATAACCAACAACGTTGCCCTACTGGCGGCCAGGATGATTTCGGTACCGCTGATGACGGTGTAACCGATGCGGTTTTGGTCGTTGATTTGGCGGTTGATTTACGTGTAATACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAAGGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAAC  >  NC_000913/2999379‑2999847
                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                  
gTTGCCCCCATCCCCCCCCTTTCCTGCCCCCGGGGCGTAAATAGGTGTACCCCGTGGAGCTGAGATATCAATCCCTTTATTGCCGCCATCTGCTGTCGAATACGGCATGATAACTTTCCCTGTCGTTGGCCATAACCAACAACGTTGCCCTACTGGCGGCCAGGATGATTTCGGTACCGCTGATGACGGTGTAACCGATGCGGTTTTGGTCGTTGATTTGGCGGTTGATTTACGTGTaa                                                                                                                                                                                                                                           <  2:716562/239‑1 (MQ=255)
               caccTTTCCTGCCCCCGCGGCGTAAATAGGTGTACCCCGTGGAGCTGAGATATCAATCCCTTTATTGCCGCCATCTGCTGTCGAATACGGCATGATAACTTTCCCTGTCGTTGGCCATAACCAACAACGTTGCCCTACTGGCGGCCAGGATGATTTCGGTACCGCTGATGACGGTGTAACCGATGCGGTTTTGGTCGTTGATTTGGCGGTTGATTTACGTGTAATACTGCTACTTTTCg                                                                                                                                                                                                                            <  1:693429/239‑1 (MQ=255)
                   tttCCTGCACCCGCGGCGTAAATAGGTGTACCCCGTGGAGCTGAGATATCAATCCCTTTATTGCCGCCATCTGCTGTCGAATACGGCATGATAACTTTCCCTGTCGTTGGCCATAACCAACAACGTTGCCCTACTGGCGGCCAGGATGATTTTGGGACCGCTGATGACGGGGTAACCGATGCGGTTTTGGGCGTTGATTTTGCGGGTGAGTTTCGGGTTAAACTGGTACTTTTTGcccc                                                                                                                                                                                                                       >  2:705721/1‑239 (MQ=255)
                    tgcctgaccccGCGGCGTAAATAGGTGTACCCCGTGGAGCTGAGATATCAATCCCTTTATGGCCGCCATCTGCTGTCGAATTCGGCATGATAACTTTCCCTGTCGTTGGCCATAACCAACAACGTTGCCCTACTGGCGGCCAGGATGATTTCGGTTCCGCTGATGACGGTGTAACCGATGCGGTTTTGGTCGTTGATTTGGCGGTTGATTTACGTGTAATGCTGCTACTTTTCGCccca                                                                                                                                                                                                                      <  1:1056214/231‑1 (MQ=255)
                      ccTGCACCCGCGGCGTAAATTGGTGTACCCCGTGGAGCTGAGATATCCATCCCTTTATTGCCGCCATCTGCTGTCGAATGCGGCATGATAACTTTCCCTGTCGTTGGCCATAACCAACAACGTTGCCCTACTGGCGGCCAGGATGATTTCGGTACCGCTGATGACGGTGTAACCGATGCGGTTTTGGTCGTTGATTTGGCGGTTGATTTACGTGTAATACTGCTACTTTTCGCcccacc                                                                                                                                                                                                                    <  1:782039/239‑1 (MQ=255)
                            cccGCGGCGTAAATAGGTGTACCCCGTGGAGCTGAGATATCAATCCCTTTATTGCCGCCATCTGCTGTCGAATACGGCATGATAACTTTCCCTGTCGTTGGCCATAACCAACAACGTTGCCCTACTGGCGGCCAGGATGATTTCGGTACCCCTGTGTACGGGGTAACCGATGGGGTTTTGGCGCTTTATTTGGCGGGTGGTTTTCGTGTGAAAATGCAAATTTTTGGcccaccccggtt                                                                                                                                                                                                              >  2:92647/1‑234 (MQ=255)
                             cccgggcgTAAATAGGTGTACCCCGTGGAGCTGAGATATCAATCCCTTTATTGCCGCCATCTGCTGTCGAATTCGGCATGATAACTTTCCCGGTCGTTGGCCATAACCAACAACGTTGCCCTACTGGCGGCCAGGATGATTTCGGTACCGCTGATGACGGTGTAACCGATGCGGTTTTGGTCGTTGATTTGGCGGTTGATTTACGTGTAATACTGCTACTTTTCGCCCCACCCAGtttt                                                                                                                                                                                                             <  2:725565/235‑1 (MQ=255)
                                    gTAAAGAGGTGTACCCGGTGGGGGTGAGATATCAATCCCTTTATTGCCGCCATCTGCTGTCGAAAACGGCATGATACCTTTCCCGGTCGTTGGCCATAACCAACAACGTTGCCCTACTGGCGGCCAGGATGATTTCGGTACCGCTGATGACGGTGTAACCGATGCGGTTTTGGTCGTTGATTTGGCGGTTGATTTACGTGTAATACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTc                                                                                                                                                                                                       <  2:546271/238‑1 (MQ=255)
                                        aTAGGTGTACCCCGTGGAGCTGAGATATCAATCCCTTTATTGCCGCCATCTGCTGTCGAATACGGCATGATAACTTTCCCTGTCGTTGGCCATAACCAACAACGTTGCCCTACTGGCGGCCAGGATGATTTCGGTACCGCTGATGACGGTGTAACCGATGCGGTTTTGGTCGTTGATTTGGCGGTTGATTTACGTGTAATACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGAcc                                                                                                                                                                                                  <  1:705721/239‑1 (MQ=255)
                                            gtgtTCCCCGTGGAGGTGAGATATCAATCCCTTTATTGCCGCCATCTGCTGTCGAATACGGCATGATAACTTTCCCTGTCGTTGGCCATAACCAACAACGTTGCCCTACTGGCGGCCAGGATGATTTCGGTACCGCTGATGACGGTGTAACCGATGCGGTTTTGGTCGTTGATTTGGCGGTTGATTTACGTGTAATACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGAcc                                                                                                                                                                                                  <  1:432384/235‑1 (MQ=255)
                                             tgtACCCCGTGGAGCTGAGATATCAATCCCTTTATTGCCGCCATCTGCTGTCGAATACGGCATGATAACTTTCCCTGTCGTTGGCCATAACCAACAACGTTGCCCTACTGGCGGCCAGGATGATTTCGGTACCGCTGATGACGGTGTAACCGATGCGGTTTTGGTCGTTGATTTGGCGGTTTGTTTTCGTGTAATACTTCTACTTTTTTGCCCACCCCGGTTTACTTTTTGTCCAACt                                                                                                                                                                                              >  1:124247/1‑238 (MQ=255)
                                             tgtACCCCGTGGAGCTGAGATATCAATCCCTTTATTGCCGCCATCTGCTGTCGAATACGGCATGATAACTTTCCCTGTCGTTGGCCATAACCAACAACGTTGCCCTACTGGCGGCCAGGATGATTTCGGTACCGCTGATGACGGTGTAACCGATGCGGTTTTGGTCGTTGATTTGGCGGTTGATTTACGGGTAATACTGCTACTTTTCCGCCCACCCAGGTTTTGTTTTTGAACaaat                                                                                                                                                                                              >  1:986310/1‑236 (MQ=255)
                                               tcccctGTGGGGCTTGGATTTCAATCCTTTTTTTTCCCCCCTCTGCTGGGTAATTCGGCATGATAACTTCCCCTGTCGTGGGCCATAACCAACAACGTTGCCCTACTGGCGGCCAGGATGATTTCGGTACCGCTGATGACGGTGTAACCGATGCGGTTTTGGTCGTTGATTTGGCGGTTGATTTACGTGTAATACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCa                                                                                                                                                                                           <  2:385019/237‑1 (MQ=255)
                                               tACCCCGTGGAGCTGAGATATCAATCCCTTTATTGCCGCCATCTGCTGTCGAATACGGCATGATAACTTTCCCTGTCGTTGGCCATAACCAACAACGTTGCCCTACTGGCGGCCAGGATGATTTCGGTACCGCTGATGACGGGGTAACCGATGCGGTTTTGGTGCTGGATTTGGCGGTTGATTTACGGTGAATCCCGCTACTTTTTGGCCCCACCAGTTTTAGTTTTTGTCCAAATTcc                                                                                                                                                                                           >  1:318542/1‑238 (MQ=255)
                                                ccccctgggAGCTGAAATATAAATCCCTTTTTTGCCCCCATCTGCTGTCGAATCCGGCATGATAACTTTCCCTGTCGTTGGCCATAACCAACAACGTTGCCCTACTGGCGGCCAGGATGATTTCGGTACCGCTGATGACGGTGTAACCGATGCGGTTTTGGTCGTTGATTTGGCGGTTGATTTACGTGTAATACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCaa                                                                                                                                                                                          <  2:1052256/232‑1 (MQ=255)
                                                 ccccGTGGAGCTGAGATATCAATCCCTTTATTGCCGCCATCTGCTGTCGAATACGGCATGATAACTTTCCCTGTCGTTGGCCATAACCAACAACGTTGCCCTACTGGCGGCCAAGATGATTTCGGTAACGCTGATACCGGTTTAACCGATGCAGGTTTTGTCGTTGATTTGTGGGGTTGTTTTTGTGGATATTACGTCACTTTTTCCCCACCCAGTTTTTGATTCTGACCaaatatcat                                                                                                                                                                                         >  2:235293/1‑232 (MQ=255)
                                                    cGTGGAGCTGAGATATCAATCCCTTTATTGCCGCCATCTGCTGTCGAATACGGCATGATAACTTTCCCTGTCGTTGGCCATAACCAACAACGTTGCCCTACTGGCGGCCAGGATGATTTCGGTACCGCTGATGACGGTGTAACCGATGCGGTTTTGGTCGTTGATTTGGCGGTTGGTTTTCGGGTAAAAATGCTACTTTTTGCCCCACCCCGGTTTAGTTTTTGACCAAATTCAAtagg                                                                                                                                                                                     >  1:377546/1‑236 (MQ=255)
                                                      tGGAGCTGAGATATCAATCCCTTTATTGCCGCCATCTGCTGTCGAATACGGCATGATAACTTTCCCTGTCGTTGGCCATAACCAACAACGTTGCCCTACTGGCGGCCCGGATGATTTCGGTACCGCTGATGCAGGTGTAACCGATGCGGGTTTGGTCGTTGATTTGGCGGTTGAATTACGTGTAAACACAGTACTTTTTGCCCCCACACCGTTTTTTTTTTGTCCAACTTccaaggggt                                                                                                                                                                                    >  2:504360/1‑231 (MQ=255)
                                                      tGGAGCTGAGATATCAATCCCTTTATTGCCGCCATCTGCTGTCGAATACGGCATGATAACTTTCCCTGTCGTTGGCCATAACCAACAACGTTGCCCTACTGGCGGCCAGGATGATTTCGGTACCGCTGATGACGGTGTAACCGATGCGGTTTTGGTCGTTGATTTGGCGGTTGGTTTTCGCGTAATACTGCTACTTTTTCCCCCACCCCGCTTTTGCTTTTGACCCACTTCCATGgtgt                                                                                                                                                                                   >  1:124809/1‑239 (MQ=255)
                                                      tGGAGCTGAGATATCAATCCCTTTATTGCCGCCATCTGCTGTCGAATACGGCATGATAACTTTCCCTGTCGTTGGCCATAACCAACAACGTTGCCCTACTGGCGGCCAGGATGATTTCGGTACCGCTGATGACGGTGTAACCGATGCGGTTTTGGTCGTTGATTTGGCGGTTGATTTACGTGTAATACTGCTACTTTTCTCCCCACCCAGTTTTAGTTTCTGACCCACTTTCATGgtgt                                                                                                                                                                                   >  1:36654/1‑239 (MQ=255)
                                                      tGGAGCTGAGATATCAATCCCTTTATTGCCGCCATCTGCTGTCGAATACGGCATGATAACTTTCCCTGTCGTTGGCCATAACCAACAACGTTGCCCTACTGGCGGCCAGGATGATTTCGGTACCGCTGATGACGGTGTAACCGATGCGGTTTTGGGCGTTGATTTGGCGGTTGATTTACGGGTAAAAATCGTAATTTTGGCCCCCCCCCGTTTTTGTTTTTGACCAACTTAAAAggggg                                                                                                                                                                                   >  2:152923/1‑236 (MQ=255)
                                                      tGGAGCTGAGATATCAATCCCTTTATTGCCGCCATCTGCTGTCGAATACGGCATGATAACTTTCCCTGTCATTGGCCATAACCAACAACGTTGCCCTACTGGCGGCCAGGATGATTTCGGTACCGCTGATGACGGTGTAACCGATGCGGGTTTGGTCGTTGATTTGGCGGTTGGTTTACGGGGAAAAATGCTACTTTTTGCCCCCCCCAAGTTTTGTTTCTGACCAAATTCaaaaggtg                                                                                                                                                                                    >  2:432384/1‑233 (MQ=255)
                                                           cTGAGATATCAATCCCTTTATTGCCGCCATCTGCTGTCGAATACGGCATGATAACTTTCCCTGTCGTTGGCCATAACCAACAACGTTGCCCTACTGGCGGCCAGGATGATTTCGGTACCGCTGATGACGGTGTAACCGATGCGGTTTTGGTCGTTGATTTGGCGGTTGATTTTCGTGTAATACTGTTACTTTTTGCCCCACCCCGTTTTAGTTTTTGTCCAACTTCAATGGGGTAAggg                                                                                                                                                                              >  1:153722/1‑239 (MQ=255)
                                                           cTGAGATATCAATCCCTTTATTGCCGCCATCTGCTGTCGAATACGGCATGATAACTTTCCCTGTCGTTGGCCATAACCAACAACGTTGCCCTACTGGCGGCCAGGATGATTTCGGTACCGCTGATGACGGTGTAACCGATGCGGTTTTGGTCGTTGATTTGGCGGTTGATTTACGTGTAATACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAAgg                                                                                                                                                                               <  1:674048/238‑1 (MQ=255)
                                                           cTGAGATATCAATCCCTTTATTGCCGCCATCTGCTGTCGAATACGGCATGATAACTTTCCCTGTCGTTGGCCATAACCAACAACGTTGCCCTACTGGCGGCCAGGATGATTTCGGTACCGCTGATGACGGTGTAACCGATGCGGTTTTGGTCGTTGATTTGGCGGTTGATTTACGTGTAATACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAAgg                                                                                                                                                                               <  1:297816/238‑1 (MQ=255)
                                                                  atCAATCCCTTTATTGCCGCCATCTGCTGTCGAATACGGCATGATAACTTTCCCTGTCGTTGGCCATAACCAACAACGTTGCCCTACTGGCGGCCAGGATGATTTCGGTACCGCTGATGACGGTGTAACCGATGCGGTTTTGGTCGTTGATTTGGCGGTTGATTTACGTGTAATACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAAGGGGGGGaaa                                                                                                                                                                       <  1:43093/239‑1 (MQ=255)
                                                                   tCAATCCCTTTATTGCCGCCATCTGCTGTCGAATACGGCATGATAACTTTCCCTGTCGTTGGCCATAACCAACAACGTTGCCCTACTGGCGGCCAGGATGATTTCGGTACCGCTGATGACGGTGTAACCGATGCGGTTTTGGTCGTTGATTTGGCGGTTGATTTACGTGTAATACTGCTACTTTTCGcc                                                                                                                                                                                                                         <  1:797000/189‑1 (MQ=255)
                                                                   tCAATCCCTTTATTGCCGCCATCTGCTGTCGAATACGGCATGATAACTTTCCCTGTCGTTGGCCATAACCAACAACGTTGCCCTACTGGCGGCCAGGATGATTTCGGTACCGCTGATGAAGGTGTAACCGATGCGGTTTTGGTCGTTGATTTGGCGGTTGATTTACGGGTAATAATGCTCCTTTTCGTCCCACCCAGTTTTATTTTCTGCCCAACATCAATGGTGTAAGGGGGGGaaa                                                                                                                                                                       >  1:355266/1‑238 (MQ=255)
                                                                          cTTTATTGCCGCCATCTGCTGTCGAATACGGCATGATAACTTTCCCTGTCGTTGGCCATAACCAACAACGTTGCCCTACTGGCGGCCAGGATGATTTAGGTAACGCTGATGACGGTGTAAAAGATGAGGTTTGGGTAGTTGATTTGGAGGGTGATTTAAGGGTAAGACTGATAAGTTGAGAAGCAAAAAGGTTTAGTTTCTGACCAACTTCAATGGTGTAAggggggg                                                                                                                                                                          <  1:201635/228‑1 (MQ=255)
                                                                           tttATTGCCGCCATCTGCTGTCGAATACGGCATGATAACTTTCCCTGTCGTTGGCCATAACCAACAACGTTGCCCTACTGGCGGCCAGGATGATTTCGGTACCGCTGATGACGGTGTAACCGATGCGGTTTTGGTCGTTGATTTGGCGGTTGTTTTACGTGGAATACTGCTACTTTTCTCCCCCCCCAGTTTTAGTTTCTTACCAACTTCAATTGGTTAAAGGGGGGGACATccctacc                                                                                                                                                               >  1:165305/1‑235 (MQ=255)
                                                                             ttttGCCCCCATCTGCTGGCCAATACTGCCTGATAACTTTCCCTGTCGTTGGCCATACCCAACAACGTTGCCCTACTGGCGGCCAGGATGATTTCGGTACCGCTGATGACGGTGTAACCGATGCGGTTTTGGTCGTTGATTTGGCGGTTGATTTACGTGTAATACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAAGGGGGGGAAATGCCGTTCAGt                                                                                                                                                            <  2:986310/237‑1 (MQ=255)
                                                                             tATTGCCGCCATCTGCTGTCGAATACGGCATGATAACTTTCCCTGTCGTTGGCCATAACCAACAACGTTGCCCTACTGGCGGCCCGGATGATTTCGGTACCGCTGATGACGGTGTAACCGATGCGGTTTTGGCCGTTGATTTGGCGGTTGATTTTCTTGTAAAACTGCTCATTTTTGCCCCACCCCCATTTTGTTTTTGACCAAATTCACATGTGTATATGGGGGaaaaaccggtttgt                                                                                                                                                            >  2:893711/1‑228 (MQ=255)
                                                                             tATTGCCGCCATCTGCTGTCGAATACGGCATGATAACTTTCCCTGTCGTTGGCCATAACCAACAACGTTGCCCTACTGGCGGCCAGGATGATTTCGGTACCGCTGATGACGGTGTAACCGATGCGGTTTTGGTCGTTGATTTGGCGGTTGATTTACGTGTAATAACTCTACTTTTTGCCCCACCCCGTTTTCATTTTTGACCACCTTCAATGGGGTAATGGGGGGGAATGCCCGTCAg                                                                                                                                                             >  1:885405/1‑238 (MQ=255)
                                                                                 gccgccATCTGCTGTCGAATACGGCATGATAACTTTCCCTGTCGTTGGCCATAACCAACAACGTTGCCCTACTGGCGGCCAGGATGATTTCGGTACCGCTGATGACGGTGTAACCGATGCGGTTTTGGTCGTTGATTTGGCGGTTGGTTTTCGTGTAATACTGCTACCTTTTGCCCCACCCCGTTTTAGTTTCTTGCCCACTTCCAAGGTGTAAGGGGGGGGAAAGCCCTTCTGTcac                                                                                                                                                        >  1:488794/1‑236 (MQ=255)
                                                                                    gccATCTGCTGTTGAATACGGCATGATAACTTTCCCTGTCGTTGGCCATAACCAACAACGTTGCCCTACTGGCGGCCAGGATGATTTCGGTACCGCTGATGACGGTGTAACCGATGCGGTTTTGGTCGTTGATTTGGCGGTTGATTTACGTGTAATACTGCTACTTTTCGCCCCACCCCGGTTTAGTTTCTGCCCAACTTCAATGGGGTAAGGGGGGGGAATGCCGTTCAGCTGCGCCa                                                                                                                                                    >  1:1085114/1‑239 (MQ=255)
                                                                                    gccATCTGCTGTCGAATACGGCATGATAACTTTCCCTGTCGTTGGCCATAACCAACAACGTTGCCCTACTGGCGGCCAGGATGATTTCGGTACCGCTGATGACGGTGTAACCGGTGCGGGTTTGGGCGTTGATTTGGCGGTTTGTTTTAGTGTAATACTTCCAATTTTTGCCCCACCCCATTTTAGTTTCTGACCAACTTCAATTGGGTAAGGGGGGGGAATGCCGTTcctgtttcttt                                                                                                                                                     >  2:201635/1‑229 (MQ=255)
                                                                                     ccATCTGCTTGCGAATACGGTATTATAACTTTCCCTGTCGTTGGCCATAACCAAAAACGTTGCCCTACGGGCGGCCAGGATGATTTCGGTACCGCTGATGACGGGGTAACCGATGCGGTTTTGGTCGTTGATTTGGCGGTTGATTTACGTGTAATACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAAGGGGGGGAAATGCCGTTCAGTCGCGCCAg                                                                                                                                                   <  2:704360/239‑1 (MQ=255)
                                                                                       aTCTGCTGTCGAATACGGCATGATAACTTTCCCTGTCGTTGGCCATAACCAACAACGTTGCCCTACTGGCGGCCAGGATGATTTCGGTACCGCTGATGACGGTGTAACCGATGCGGTTTTGGTCGTTGATTTGGCGGTTGATTTACGTGTAATACTGCTAATTTTCGCCCCACCCAGGTTTTTTTTCTGACCAACTTCAATGGTGTAAGGGGGGGAAATACCGTTCAGTCGCGCCAggt                                                                                                                                                 >  1:228522/1‑237 (MQ=255)
                                                                                                 gatttcGGCATGATTACTTTCCCTTTCGTTGGCCATAACCAACAACGTTGCCCTACTGGCGGCCAGGATGATTTCGGTACCGCTGATGACGGTGTAACCGATGCGGTTTTGGTCGTTGATTTGGCGGTTGATTTACGTGTAATACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAAGGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCt                                                                                                                                       <  2:1009356/234‑1 (MQ=255)
                                                                                                 gAATCCGGCATGATAACTTTCCCGGTCGTTGGCCATAACCAACAACGTTGCCCTACTGGCGGCCAGGATGATTTCGGTACCGCTGATGACGGTGTAACCGATGCGGTTTTGGTCGTTGATTTGGCGGTTGATTTACGTGTAATACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAAGGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCt                                                                                                                                       <  1:640279/239‑1 (MQ=255)
                                                                                                  aaTCCGGCATGATAACTTTCCCTGTCGTTGGCCATAACCAACAACGTTGCCCTACTGGCGGCCAGGATGATTTCGGTACCGCTGATGACGGTGTAACCGATGCGGTTTTGGTCGTTGATTTGGCGGTTGATTTACGTGTAATACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAAGGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCt                                                                                                                                       <  1:812380/238‑1 (MQ=255)
                                                                                                            gATAACTTTCCCTGTCGTTGGCCATAACCAACAACGTTGCCCTACTGGCGGCCAGGATGATTTCGGTACCGCTGATGACGGTGTAACCGATGCGGTTTTGGTCGTTGATTTGGCGGTTGATTTACGTGTAATACTGCTACTTTTCGCCCCACCCAGTTTTAATTTCTTACCAACTTCAATGGGGTAAGGGGGGGAACTGCCCTTCAGTCGTGCCCGTTCTTTTTTGCCGCTTccccggg                                                                                                                            >  1:623726/1‑235 (MQ=255)
                                                                                                                  tttCCCTGTCGTTGGCCATAACCAACAACGTTGCCCTACTGGCGGCCAGGATGATTTCGGTACCGCTGATGACGGTGTAACCGATGCGGTTTTGGTCGTTGATTTGGCGGTTGATTTACGTGTAATACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAAGGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGcgcg                                                                                                                      <  1:706875/239‑1 (MQ=255)
                                                                                                                  tttCCCTGTCGTTGGCCATAACCAACAACGTTGCCCTACTGGCGGCCAGGATGATTTCGGTACCGCTGATGACGGTGTAACCGATGCGGTTTTGGTCGTTGATTTGGCGGTTGATTTACGTGTAATACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAAGGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGcgcg                                                                                                                      <  1:96718/239‑1 (MQ=255)
                                                                                                                    tcccgatCGTTGGCCCAAGACAACAACGTTGCCCTACTGGCGGCCAGGATGATTTCGGTACCGGTGATGACGGTGTAACCGATGCGGTTTTGGTCGTTGATTTGGCGGTTGATTTACGTGTAATTCTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAAGGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGaa                                                                                                                    <  1:782151/233‑1 (MQ=255)
                                                                                                                    tCCCTTTGGTTGGCCATAACCAACAACGTTGCCCTACTGGGGGCCCGGATGATTTCGGTACCGCGGATGACGGTGTAACCGGTGCGGTTTTGGTCGTTGATTTGGCGGTTGATTTACGTGTAATACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAAGGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGaa                                                                                                                    <  2:124809/239‑1 (MQ=255)
                                                                                                                      ctttttgTTTGCCATAACCAACAACGTTGCCCTTCTGGCGGCCAGGATGATTTCGGTACCGGTGATGGCGGTGTAACCGATGCGGTTTTGGTCGTTGATTTGGCGGTTGATTTACGTGTAATACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAAGGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAAt                                                                                                                  <  2:127801/233‑1 (MQ=255)
                                                                                                                       cTGTCGTTGGACATAACCAACAACGTTGCCCTACTGGCGGCCAGGATGATTTCGGTACCGCTGATGACGGTGTAACCGATGCGGTTTTGGTCGTTGATTTGGCGGTTGATTTACGTGTAATACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAAGGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATa                                                                                                                 <  1:687210/239‑1 (MQ=255)
                                                                                                                          tCGTTGGCCATAACCAACAACGTTGCCCTACTGGCGGCCAGGATGATTTCGGTACCGCTGATGACGGTGTAACCGATGCGGTTTTGGTCGTTGATTTGGCGGTTGATTTACGGGTAATACTGCTACTTTTTGCCCCACCCAGTTTTAGTTTTTGACCAACTTCAATGGGGTAAGGGGGGGAAATGCCGTTCAGTCCGCCCCGGTTTTTTACGCTGGTTCCCCTGGGTGGCGGAATAccg                                                                                                              >  1:248154/1‑237 (MQ=255)
                                                                                                                           cGTTGGCCATAACCAACAACGTTGCCCTACTGGCGGCCAGGATGATTTCGGTACCGCTGATGACGGTGTAACCGATGCGGTTTTGGTCGTTGATTTGGCGGTTGATTTACGTGTAATACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAAGGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGa                                                                                                              <  1:16909/238‑1 (MQ=255)
                                                                                                                            gtgagCCATACCCAACAACTTTGCCCCCCTGGGTGCACGGATGATTGCGGTGACGCGTATGAGGGTGTAACCGATGGGGTTTTGGTCGGTGATTTGGCGTTTGATTTACGTGTAATACTGCTATTTTTCGCCCCTCCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAAGGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGGTCCCGTGGTGCGCGAAATACGata                                                                                                            <  2:963544/235‑1 (MQ=255)
                                                                                                                                    tAACCAACAACGTTGCCCTACTGGCGGCCAGGATGATTTCGGTACCGCTGATGACGGTGTAACCGATGCGGTTTTGGTCGTTGATTTGGCGGTTGATTTACGTGTAATACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGGGTAAGGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGGTCCCGTGGGGCGCGAAAAAGGAtataaaggt                                                                                                     >  1:584708/1‑233 (MQ=255)
                                                                                                                                      aCCAACAACGTTGCCCTACGGGCGGCCAGGATGATTTCGGTACCGCTGATGACGGTGTAACCGATGCGGTTTTGGTCGTTGATTTGGCGGTTGATTTACGTGTAATACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAAGGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATcc                                                                                                  <  1:687661/239‑1 (MQ=255)
                                                                                                                                        caaccaCGTTGCCCTACCGGCGGCCGGGATTGTTTCGGTACCGTTTATGACGGTGTAACCGATGGGGTTTTGGTCGGTGATTTGGCGGTTGATTTACGTGTAATACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAAGGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATcccc                                                                                                <  2:248154/239‑1 (MQ=255)
                                                                                                                                          acaacGTTGCCCTACTGGCGGCCAGGATGATTTCGGTACCGCTGATGACGGTGTAACCGATGCGGTTTTGGTCGTTGATTTGGCGGTTGATTTACGTGTAATACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAAGGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGGTCCCGTGGTGCGCGGAATACGATATAGCGGATccccc                                                                                               >  1:806407/1‑238 (MQ=255)
                                                                                                                                           caacGTTGCCCTACTGGCGGCCAGGATGATTTCGGTACCGCTGATGACGGTGTAACCGATGCGGTTTTGGTCGTTGATTTGGCGGTTGATTTACGTGTAATACTGCTACTTTTCGcc                                                                                                                                                                                                                         <  2:972730/117‑1 (MQ=255)
                                                                                                                                           caacGTTGCCCTACTGGCGGCCAGGATGATTTCGGTACCGCTGATGACGGTGTAACCGATGCGGTTTTGGTCGTTGATTTGGCGGTTGATTTACGTGTAATACTGCTACTTTTCGcc                                                                                                                                                                                                                         >  1:972730/1‑117 (MQ=255)
                                                                                                                                                 tGCCCTACTGGCGGCCAGGATGATTTCGGTACCGCTGATGACGGTGTAACCGATGCGGTTTTGGTCGTTGATTTGGCGGTTGATTTACGTGTAATACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAAGGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCAc                                                                                       <  1:114251/239‑1 (MQ=255)
                                                                                                                                                  gCCCTACTGGCGGCCAGGATGATTTCGGTACCGGTGATGACGGTGTAACCGATGCGGTTTTGGTCGTTGATTTGGCGGTTGATTTACGTGTAATACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAAGGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCACg                                                                                      <  1:92647/239‑1 (MQ=255)
                                                                                                                                                         tGGCGGCCAGGGTTGTTTCGGTACCGCTGATGACGGTTTAACCGATGCGGTTTTGGTCGTTGATTTGGCGGTTGATTTACGTGTAATACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAAGGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTaaa                                                                               <  2:153722/239‑1 (MQ=255)
                                                                                                                                                             gggcggggttgtttCGGGACCGCTGGTGACGGGGTAACCGATGGGGTTTTGGTCGTTGATTTGGCGGTTGATTTACGTGTAATACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAAGGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACgg                                                                            <  2:584708/227‑1 (MQ=255)
                                                                                                                                                             ggcCAGGATGATTTCGGTACCGCTGATGACGGTGTAACCGATGCGGTTTTGGTCGTTGATTTGGCGGTTGATTTACGTGTAATACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAAGGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAACGTATTCCCCCGTTTCACGGTGTAAACGGa                                                                           >  1:553201/1‑239 (MQ=255)
                                                                                                                                                                     ttgtttCGGTACCGTTTTTGGCGGTGTAACCGATGCGGTTTTGGTCGTTGATTTGGCGTTTGATTTTCGTGTAATACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAAGGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGaa                                                                    <  2:318542/235‑1 (MQ=255)
                                                                                                                                                                                 cGCTGATGACGGTGTAACCGATGCGGTTTTGGTCGTTGATTTGGCGGTTGATTTACGTGTAATACTGCTACTTTTCGCCCCACCCCGTTTTAGTTTCTGACCAACTTCAATGGGGTAAGGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGGGGTGCGCGAAATACGCTATAGCGTATCCCCCCGTTTCACGTTGTAAACGGAGCCGGAATTAGTTCCTGtt                                                        >  1:104237/1‑237 (MQ=255)
                                                                                                                                                                                   gtgatgaCGGTGTAACCGATGCGGTTTTGGTCGTTGATTTGGCGGTTGATTTACGTGTAATACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAAGGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCa                                                                                        <  2:311662/203‑1 (MQ=255)
                                                                                                                                                                                   cTGATGACGGTGTAACCGATGCGGTTTTGGTCGTTGATTTGGCGGTTGATTTTCGGGTAATACTGCTACTTTTCGCCCCACCCCGTTTTAGTTTCTGACCAACTTCAATGGTGTAATGGGGGGAAATGCCGTTCACTCGCGCCCGTTCTTTTTCGCTGGTTCCCGTGGGTCGCGGAATCAGAAATAGCGTATTCCCCCGTTTCCCCGGGTAAACGGACCCGGAAAAAGTTCCGGTAACg                                                     >  2:751577/1‑239 (MQ=255)
                                                                                                                                                                                   cTGATGACGGTGTAACCGATGCGGTTTTGGTCGTTGATTTGGCGGTTGATTTACGTGTAATACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAAGGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTcc                                                                                        >  1:311662/1‑203 (MQ=255)
                                                                                                                                                                                      atgaCGGTGTAACCGATGCGGTTTTGGTCGTTGATTTGGCGGATGATTTACGTGTAATAATGCTACTTTTCGCCCCCCCCCGTTTTAGTTTCTGACCCACTTCAATGGTGTAAGGGGGGGAAATGCCGGTCAGTCGCCCCCGTTCTTTTACGTCGGTTTCCGGGGGGGCCGGAATTAGATATCCCGTATCTCCCCGGTCCACGGGGCAAACGCGGCCGGACAACATTccccaatccgcg                                                  >  2:17797/1‑229 (MQ=255)
                                                                                                                                                                                           ggTGTAACCGATGCGGTTTTGGTCGTTGATTTGGCGGTTGATTTACGTGTAATACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAAGGGGGGGAAATTCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGGTGAtt                                              >  1:164526/1‑238 (MQ=255)
                                                                                                                                                                                              gttaccgtttgggTTTTGGGGGTTGAGTTGGCGGTTGATTTACGTGTACATCCGCTATTTGTCGCCCCACCCAGTTTTAGTTGCTGACCTACTTCAAGGGTGTAAGGGGGGGAAATGCCGTTCAGGCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGc                                           <  2:370448/227‑1 (MQ=255)
                                                                                                                                                                                                acactgtGCGGTTTTGGTCGTTGTTTTTGCGGTTGAGTTTCGGTTAATTATTCTCCTTGTCGACCCGCCCATTTTTAGTTTCTGACCAACTTCAATGGTGTAAGGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTAc                                        <  2:36654/233‑1 (MQ=255)
                                                                                                                                                                                                      tGCGGTTTTGGTCGTTGATTTGGCGGTTGATTTACGTGTAATACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATTGTGTAAGGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATTTAGCGTATCCCCCCCTTTCCCGGTTTAAACGGACACGGGAAACGTTTCTGTATCGTGTGATTTTCTACCCGaca                                  >  2:305083/1‑237 (MQ=255)
                                                                                                                                                                                                       gCGGTTTTGGTCGTTGATTTGGCGGTTGATTTACGTGTAATACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAAGGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTTCGCGAAATACGGTATAGCGTATCCCCCCGTTTCACGGTGTAAACGGACCCGGAATACGTTCCTGTATCGGAGGATTTCTTACCCGCAc                                  >  1:643141/1‑238 (MQ=255)
                                                                                                                                                                                                       gCGGTTTTGGTCGTTGATTTGGCGGTTGATTTACGTGTAATACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAAGGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGAATCCCCCCGTTTCACGGGGTAAAAGGAGCTGGAATACGTTCCTGTAAAGGATAATTTGCTACCCGAAc                                  >  1:194320/1‑238 (MQ=255)
                                                                                                                                                                                                         ggTTTTGGTCGTTGATTTGGCGGTTGATTTACGTGTAATACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAAGGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGc                               <  1:152923/239‑1 (MQ=255)
                                                                                                                                                                                                         ggTTTTGGTCGTGGATTGGGGGGTTGATTTGAGTGTAATATTGCGGCTTTGAGCCCCAGTCCGGTATAGTTTCTGAGCCCAGTCAATGGAGTACGCGGTGGAACGGCCGATCAATCGCGTCAGTGTTCTGAGGCGGGTTCCGGTGGTGCGCGAAATACGATATAGCGTATCCCCGAGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGc                               <  2:782401/239‑1 (MQ=255)
                                                                                                                                                                                                                gTCGTTGATTTGGCGGTTGATTTACGTGTAATACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAAGGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGAAATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGGAAACGTTCCTGTATCGGGTGATTTGCTCACCGAACAGCCCGCCaa                        >  1:647277/1‑239 (MQ=255)
                                                                                                                                                                                                                                       tACGTGTAATACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAAGGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTTCTACCCGAACAGCCCCCCAAAAGCAGTCCAAACGAAaaaaac  >  2:132811/1‑239 (MQ=255)
                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                  
GTTGCCCACATACACCACCTTTCCTGCACCCGCGGCGTAAATAGGTGTACCCCGTGGAGCTGAGATATCAATCCCTTTATTGCCGCCATCTGCTGTCGAATACGGCATGATAACTTTCCCTGTCGTTGGCCATAACCAACAACGTTGCCCTACTGGCGGCCAGGATGATTTCGGTACCGCTGATGACGGTGTAACCGATGCGGTTTTGGTCGTTGATTTGGCGGTTGATTTACGTGTAATACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAAGGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAAC  >  NC_000913/2999379‑2999847

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: