Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	3,219,741	A→G	15.5%	E83G (GAG→GGG)	patA →	putrescine:2‑oxoglutaric acid aminotransferase, PLP‑dependent

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,219,741	0	A	G	15.5%	187.4 / 2.1	88	E83G (GAG→GGG)	patA	putrescine:2‑oxoglutaric acid aminotransferase, PLP‑dependent
Reads supporting (aligned to +/- strand):  ref base A (39/32);  new base G (12/4);  total (51/37)
Fisher's exact test for biased strand distribution p-value = 1.69e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TCGAGCGCATCGGCTTTAGCGTGCAGCGCCCACGCCCTGAATCTCATTGAGAAGCGAACGCTGGATCATGAGGAGATGAAAGCACTTAACCGAGAGGTGATTGAATACTTCAAAGAGCATGTCAATCCGGGGTTTTTAGAGTATCGCAAATCTG‑TTACCGCC‑GGCGGGGATTACGGAGCCGTAGAGTGGCAAGCGGGAAGTTTAAATACGCTTGTCGACACCCAG‑GGCCAGGAGTTTATCGACTGCCTGGGAGGTTTTGGAATTTTCAACGTGGGGCACCGTAATCCA‑GTTGTGGTTTCCGCCGTACAGAATCAACTTGCGAAACAACCGCTGCAC‑AGCCAGGAGCTGCTCGATCCGTTACGGGCGATGTTGGCGAAAACCCTTGCTGCGCTAACGCCCGGTAAACTGAAATACAGCTTCTTCTGTAATAGCGGCACCGAGTCCGTTGAAGCAGCGCTGAAGC  >  NC_000913/3219509‑3219971                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                         tCGAGCGCATCGGCTTTAGCGTGCAGCGCCCACGCCCTGAATCTCATTGAGAAGCGAACGCTGGATCATGAGGAGATGAAAGCACTTAACCGAGAGGTGATTGAATACTTCAAAGAGCATGTCAATCCGGGGTTTTTAGAGTATCGCAAATCAG‑TTACCGCC‑GGCGGGGATTACGGAGCCGTAAAGTGGCAAGCGGGAAGGTTAAATACGCTTGTCGCAACCCAG‑GGCCAGGGGTTTaa                                                                                                                                                                                                                                    >  2:1088650/1‑238 (MQ=255)    ggcgcCTGGGCTTTGGCTGGCACCGCCCACGCCCTGAATCTAATGGAGAAGGGAGCGTTGGTTCATGAGGAGATGAAGGCACTTAACCGAGAGGTGATTGAATACTTCAAAGAGCATGTCAATCCGGGGTTTTTAGAGTGTGGCAAATCTG‑TGACCGCC‑GGCGGGGATTACGGAGCCGTAGAGTGGCAAGCGGGAAGTTTAAATACGCTTGTCGACACCCAG‑GGCCAGGAGTTTATCGa                                                                                                                                                                                                                                 <  2:574078/238‑1 (MQ=255)    aGCGCATCGGCTTTAGCGTGCAGCGCCCACGCCCTGAATCTCATTGAGAAGCGAACGCTGGATCATGAGGAGATGAAAGCACTTAACCGAGAGGTGATTGAATACTTCAACGAGCATGTCAATCCGGGGTTTTTAGAGTATCGCAAATCTG‑TTACCGCC‑GGCGGGGATTACGGAGCCGTAGAGTGGCAAGCGGGAAGTTTAATTACGCTTGTCGACACCCAG‑GGCCAGGGGTTTTTCGa                                                                                                                                                                                                                                 >  1:579092/1‑239 (MQ=255)     gcgcATCGGCTTTAGGGGGCAGCGCCCACGCCTTGAATCTGATTGAGAAGCGAACGGTGGATCATGAGGAGATGAAAGCCCTTAACCGAGAGGTGATTGAATTCTTCAAAGAGCATGTCAATCGGGGGTTTTTAGAGTATCGCAAATCTG‑TTACCGCC‑GGCGGGGATTACGGAGCCGTAGAGTGGCAAGCGGGAAGTTTAAATACGCTTGTCGACACCCAG‑GGCCAGGAGTTTATCGa                                                                                                                                                                                                                                 <  1:58863/238‑1 (MQ=255)     gcgcATCGGCTTTAGCGTGCAGCGCCCCCGCCCTGAATCTCATTGAGAAGCGAACGCTGGATCATGAGGAGATGAAAGCACTTAACCGAGAGGTGATTGAATACTTCAAAGAGCATGTCAATCCGGGGTTTTTAGAGTATCGCAAATCTG‑TTACCGCC‑GGCGGGGATTACGGAGCCGTAGAGTGGCAAGCGGGAAGTTTAAATACGCTTGTCGACACCCAG‑GGCCAGGAGTTTATCGa                                                                                                                                                                                                                                 <  1:980990/238‑1 (MQ=255)       gctctggCTTTAGCGGCCAGCGCCCACTCCCTGAATTTCATTGAGAAGCGAACGCGGGATTATGAGGAAATGAAAGCATTGACCCGAGAGGTGATTGAATACTTCAAAGAGCATGGCAATCCGGGGTTTTTAGAGTATCGCAAATCTT‑TTACCGCC‑GGCGGGGATTACGGAGCCGTAGAGTGGCAAGCGGGAAGTTTAAATACGCTTGTCGACACCCAG‑GGCCAGGAGTTTATCGAc                                                                                                                                                                                                                                <  2:148055/232‑1 (MQ=255)             gttttAGGGTGCGGCGCCCACGCCCTGAATCTAATTGGAAAGCGAACGGTGGATCATGAGGGAATGAAAGCCCTTAACTGAGGGGGGATTGAATACTTCAAAGAGCATGGCAATCCGGGGTTTTTAGAGTATCGCAAATCTG‑TTACCGCC‑GGCGGGGATTACGGAGCCGTAGAGTGGCAAGCGGGAAGTTTAAATACGCTTGTCGACACCCAG‑GGCCAGGAGTTTATCGACTGCCTggg                                                                                                                                                                                                                        <  2:792751/237‑1 (MQ=255)              cttttggggtcAGCCCCCAAGCCCTGAATTCCATTGATAAGCGAACGTTGGATCATGAGGAGATGAAAGCACTTAACCGAGAGGTGATTGAATACTTCAAAGAGCATGTCAATCCGGGGTTTTTAGAGTATCGCAAATCTG‑TTACCGCC‑GGCGGGGATTACGGAGCCGTAGAGTGGCAAGCGGGAAGTTTAAATACGCTTGTCGACACCCAG‑GGCCAGGAGTTTATCGACTGCCTggg                                                                                                                                                                                                                        <  1:735130/228‑1 (MQ=255)              cTTTGGCGTGCAGCGCCCACGCCCGGAATCTCATTGAGAAGCGAACGCTGGATCAGGAGGAGATGAAAGCACTTAACCGAGAGGTGATTGAATACTTCAAAGAGCATGTCAATCCGGGGTTTTTAGAGTATCGCAAATCTG‑TTACCGCC‑GGCGGGGATTACGGAGCCGTAGAGTGGCAAGCGGGAAGTTTAAATACGCTTGTCGACACCCAG‑GGCCAGGAGTTTATCGACTGCCTggg                                                                                                                                                                                                                        <  1:219784/238‑1 (MQ=255)                       gCAGCGCCCACGCCCTGAATCTCATTGAGAAGCGAACGCTGGATCATGAGGAGATGAAAGCTCTTAACCGAGAGGTGATTGAATACTTCAAAGAGCATGTCAATCCGGGGTTTTTAGGGAATCGCAAATCTG‑TTACCGCC‑GGCGGGGATTACGGGGCCGTAGAGTGGCAAGCGGGAAGTTTAAATACGCTTGTCGACACCCAG‑GGCCAGGGGTTTATGGCATGCCTGGGAGGTTTTGGa                                                                                                                                                                                                              >  1:948109/1‑239 (MQ=255)                        cAGCGTCCACGCCCTGAATCTCATTGAGAAGCGAACGCTGGATCATGAGGAGATGAAAGCACTTAACCGAGAGGTGATTGAATACTTCAAAGAGCATGTCAATCCGGGGTTTTTAGAGTATCGCAAATCTG‑TTACCGCC‑GGCGGGGATTACGGAGCCGTAAAGTGGCAAACGGGAAGTTTAAATACGCTTGTCGCCACCCAG‑GGCCAGGAGTTTATCGACGGCCTGGGGGGTTTTTGaa                                                                                                                                                                                                             >  1:819162/1‑239 (MQ=255)                        cAGCGCCCACGCCCTGAATCTCATTGAGAAGCGAACGCTGGATCATGAGGAGATGAAAGCACTTAACCGAGAGGTGATTGAATACTTCAAAGAGCATGTCAATCCGGGGCTTTTAGAGTATCGCAAATCTG‑TTACCGCC‑GGCGGGGATTACGGAGCCGTAGAGTGGCAAGCGGGAAGTTTAAATAAGCTTGTCGACACCCAG‑GGCCAGGAGTTTAACGGCCTGCTGGGAGGGTTTGGaa                                                                                                                                                                                                             >  2:697031/1‑239 (MQ=255)                                 gccccTGAATCCAATTGAGAAGCGAACGCTGGATCATGAGGTAGTGAAAGCCCTTAACCGAGAGGTGATTGAATCCTTCAAAGAGAATGTCAATCCGGGGTTTTTAGAGTATCGCAAATCTG‑TTACCGCC‑GGCGGGGATTACGGAGCCGTAGAGTGGCAAGCGGGAAGTTTAAATACGCTTGTCGACACCCAG‑GGCCAGGAGTTTATCGACTGCCTGGGAGGTTTTGGAATTTTCAACg                                                                                                                                                                                                    <  1:805230/237‑1 (MQ=255)                                      ggAATCTCATTGAGAAGCGAACGCTGGATCATGAGGAGATGAAAGCACTTAACCGAGAGGTGATTGAATACTTCAAAGAGCATGTCAATCCGGGGTTTTTAGAGTATCGCAAATCTG‑TTACCGCC‑GGCGGGGATTACGGAGCCGTAGAGTGGCAAGCGGGAAGTTTAAATACGCTTGTCGACACCCAG‑GGCCAGGAGTTTATCGACTGCCTGGGAGGTTTTGGAATTTTCAACGTgggg                                                                                                                                                                                               <  1:846992/238‑1 (MQ=255)                                            tcATTGAGAAGCGAACGCTGGATCATGAGGAGATGAAAGCACTTAACCGAGAGGTGATTGAATACTTCAAAGAGCATGTCAATCCGGGGTTTTTAGAGTATCGCAAATCTG‑TTACCGCC‑GGCGGGGATTACGGAGCCGTAGAGGGGCAAGCGGGAAGTTTAAAAACGCTTGTCGAACCCCAG‑GGCCAGGGGTTTTTCGACTGCCTGGGAGGTTTTGGAATTTTCAACCGGGGGGAcggg                                                                                                                                                                                         >  2:846992/1‑236 (MQ=255)                                              aTTGAGAAGCGAACGCTGGATCATGAGGAGATGGAAGCACTTAACCGAGAGGTGATTGAATACTTCAAAGAGCATGTCAATCCGGGGTTTTTAGAGTATCGCAAATCTG‑TTACCGCC‑GGCGGGGGTTACGGAGCCGTAGAGTGGCAACCGGGAGGTTTAAATACGCTTGTCGCAACCCAG‑GGCCCGGGGTTTATCGATGGCCGGGGGGGGTTTTGAATTTTCAACGTGGGGcccgggaa                                                                                                                                                                                       >  2:972700/1‑232 (MQ=255)                                              aTTGAGAAGCGAACGCTGGATCATGAGGAGATGAAAGCACTTAACCGAGAGGTGATTGAATACTTCAAAGAGCATGTCAATCCGGGGTTTTTAGAGTATCGCAAATCTG‑TTACCGCC‑GGCGGGGATTACGGAGCCGTAGAGTGGCAAGCGGGAAGTTTAAATACGCTTGTCGAAACCCAG‑GGGCAGGAGTTTATCGCGTGCCCGGGGGGTTTTGGAATTTTTAACGGGGGGGAcccaaa                                                                                                                                                                                       >  2:652114/1‑235 (MQ=255)                                                tGAGAAGCGAACGCTGGATCATGAGGAGATGAAAGCACTTAACCGAGAGGTGATTGAATACTTCAAAGAGCATGTCAATCCGGGGTTTTTAGAGTATCGCAAATCTG‑TTACCGCC‑GGCGGGGATTACGGAGCCGTAGAGTGGCAAGCGGGAAGTTTTAATACGCTTGTCGACACCCCG‑GGCCAGGAGTTTATCGCCTGCCTGGGAGGGTTTGGAATTTTTAACGTGGGGCACCGGAAt                                                                                                                                                                                      >  1:282131/1‑238 (MQ=255)                                                tGAGAAGCGAACGCTGGATCATGAGGAGATGAAAGCACTTAACCGAGAGGTGATTGAATACTTCAAAGAGCATGTCAATCCGGGGTTTTTAGAGTATCGCAAATCTG‑TTACCGCC‑GGCGGGGATTACGGAGCCGTAGAGTGGCAAGCGGGAAGTTTAAATACGCTTGTCGCCACCCAG‑GGCCAGGGGTTTATCGACTGCCTGGGAGGTTTTGGAATTTTTAAACGGGGGCACCGTAAt                                                                                                                                                                                      >  1:562286/1‑238 (MQ=255)                                                tGAGAAGCGAACGCTGGATCATGAGGAGATGAAAGCACTTAACCGAGAGGTGATTGAATACTTCAAAGAGCATGTCAATCCGGGGTTTTTAGAGTATCGCAAATCTG‑TTACCGCC‑GGCGGGGATTACGGAGCCGTAGAGTGGCAAGCGGGAAGTTTAAATACGCTTGTCGACACCCAC‑GGCCAGGGGTTTATCGACTGCCTGGGCGGTTTTGGGATTTTCAACGGGGGGGCCCGTAAt                                                                                                                                                                                      >  2:653478/1‑238 (MQ=255)                                                     aGCGAACGCTGGATCATGAGGAGATGAAAGCACTTAACCGAGAGGTGATTGAATACTTCAAAGAGCATGTCAATCCGGGGTTTTTAGAGTTTCGCAAATCTG‑TTACCGCC‑GGCGGGGATTACGGAGCCGTAGAGTGGCAAGCGGGAAGTTTAAATACGCTTGTCGACACCCAG‑GGCCAGGAGTTTATCGACTGCCTGGGAGGTTTTGGAATTTTCAACGTGGGGCACCGTAATCCA‑Gtt                                                                                                                                                                               <  1:458076/239‑1 (MQ=255)                                                       cGAACGCTGGATCATGAGGAGATGAAAGCACTTAACCGAGAGGTGATTGAATACTTCAAAGAGCATGTCAATCCGGGGTTTTTAGAGTATCGCAAATCTG‑TTACCGCC‑GGCGGGGATTACGGAGCCGTAGAGTGGCAAGCGGGAAGTTTAAATACGCTTGTCGACACCCAG‑GGCCAGGGGTTTATCGACTGCCTGGGGGGTTTTGGAATTTTCAACGTGGGGCACCGGAATCCA‑GTtgt                                                                                                                                                                             >  1:581375/1‑239 (MQ=255)                                                        gAACGCTGGATCATGAGGAGATGAAAGCACTTAACCGAGAGGTGATTGAATACTTCAAAGAGCATGTCAATCCGGGGTTTTTAGAGTATCGCAAATCTG‑TTACCGCC‑GGCGGGGATTACGGGGCCGTAGAGTAGCAAGCGGGAAGTTTAAATACGCTTGTCGACACCCAG‑GGACAGGGGGTTTTCGCCTGCCTGGGGGGTTTTGGAATTTTCAACGCGGGGCACCCTAATCCC‑Gtgttg                                                                                                                                                                            >  2:240862/1‑235 (MQ=255)                                                                gATCATGAGGAGATGAAAGCACTTAACCGAGAGGTGATTGAATACTTCAAAGAGCATGTCAATCCGGGGTTTTTAGAGTATCGCAAATCTG‑TTACCGCC‑GGCGGGGATTACGGAGCCGTAGAGTGGCAAGCGGGAAGTTTAAATACGCTTGTCGACACCCAG‑GGCCAGGAGTTTATCGACTGCCTGGGGGGGTTTGGAATTTTCAACGGGGGGCACCGTAATCCA‑CTTGGGGTTTcct                                                                                                                                                                     >  1:403145/1‑237 (MQ=255)                                                                      gaggaaATGAAAGCATTTAACCGAAAGGTGATGGAATTCTTCAAAGAGCATGTAAATCCGGGGTTTTTAGAGTATCGAAAATCTG‑TTACCGCC‑GGCGGGGATTACGGAGCCGTAGAGTGGCAAGCGGGAAGTTTAAATACGCTTGTCGACACCCAG‑GGCCAGGAGTTTATCGACTGCCTGGGAGGTTTTGGAATTTTCAACGTGGGGCACCGTAATCCA‑GTTGTGGTTTCCGCCGTACa                                                                                                                                                              <  1:483011/239‑1 (MQ=255)                                                                        ggagATGAAAGCACTTAACCGAGAGGTGATTGAATACTTCAAAGAGCATGTCAATCCGGGGTTTTTAGAGTATCGCAAATCTG‑TTACCGCC‑GGCGGGGATTACGGAGCCGTAGCATGGCAAACGGGAAGTTTAAATACGGTTGTCGACACCCAG‑GGCACGGAGTTTATCGACTTCCTGGGGGGGTTTGGAATTTTCAAAGTGGGGGCACGACATCCC‑GTTGTGGTTTGCGCCATACAGa                                                                                                                                                            >  2:665307/1‑239 (MQ=255)                                                                         gagaTGAAAGCACTTAACCGAGAGGTGATTGAATCCTTCAAAGAGCATGTCAATCCGGGGTTTTGAGATTATCGCAAATCGG‑TTACCGCC‑GGCGGGGATTACGGAGCCGTAGAGTGGCAAGCGGGAAGTTTAAATACGCTTGTCGACACCCAG‑GGCCAGGAGTTTATCGACTGCCTGGGAGGTTTTGGAAtttt                                                                                                                                                                                                         <  1:945654/194‑1 (MQ=255)                                                                           gaTGAAAGCACTTAACCGAGAGGTGATTGAATACTTCAAAGAGCATGTCAATCCGGGGTTTTTAGAGTATCGCAAATCTG‑TTACCGCC‑GGCGGGGATTACGGAGCCGTAGAGTGGCAAGCGGGAAGTTTAAATACGCTTGTCGACACCCAG‑GGCCAGGAGTTTATCGACTGCCTGGGGGGCTTTGGAATTTTTAACGGTGGGCACCGTAATCCA‑GTTGTGGGTTCCGCCGTACAGAATc                                                                                                                                                         >  1:500999/1‑239 (MQ=255)                                                                            aTGAAAGCACTTAACCGAGAGGTGATTGAATACTTCAAAGAGCATGTCAATCCGGGGTTTTTAGAGTATCGCAAATCTG‑TTACCGCC‑GGCGGGGATTACGGAGCCGTAGAGTGGCAAGCGGGAAGTTTAAATACGCTTGTCGACACCCAG‑GGCCCGGGGTTTATCGACTGCCTGGGAGGTTTTGGAATTTTCAACCTGGGGCACCGCAATCCA‑GTGTTGGCTTCCGCCGTACCGAAtaa                                                                                                                                                        >  1:1088405/1‑237 (MQ=255)                                                                            aTGAAAGCACTTAACCGAGAGGTGATTGAATACTTCAAAGAGCATGTCAATCCGGGGTTTTTAGAGTATCGCAAATCTG‑TTACCGCC‑GGCGGGGATTACGGAGCCGTAGAGTGGCAAGCGGGAAGTTTAAATACGCTTGTCGACACCCAG‑GGCCAGGAGTTTATCGCCTGCATGGGGGGTTTTGGAATTTTCAACGTTGGCGACAGTAATCCA‑GTTGTGGTTTCCGCCGTTCAGatta                                                                                                                                                         >  1:902769/1‑235 (MQ=255)                                                                            aTGAAAGCACTTAACCGAGAGGTGATTGAATACTTCAAAGAGCATGTCAATCCGGGGTTTTTAGAGTATCGCAAATCTG‑TTACCGCC‑GGCGGGGATTACGGAGCCGTAGAGTGGCAAGCGGGAAGTTTAAATACGCTTGTCGACACCCAG‑GGCCAGGAGTTTATCGACTGCCTGGGAGGTTTTGGAATTTTCAACGTGGGGCACCGTAATCCA‑GTTGGGGTTTCCGCCGTaaaaaacaa                                                                                                                                                        >  2:895373/1‑231 (MQ=255)                                                                            aTGAAAGCACTTAACCGAGAGGTGATTGAATACTTCAAAGAGCATGTCAATCCGGGGTTTTTAGAGTATCGCAAATCTG‑TTACCGCC‑GGCGGGGATTACGGAGCCGTAGAGGGGCAAGCGGGAAGTTTAAATACGCTTGTCGACACCCAG‑GGCCAGGGGTTTATCGACTGCCTGGGAGGTTTTGGAATTTTCAAAGTGGGGCACCGTAATCCA‑GTTTGGGTTTccgccggtacgaatca                                                                                                                                                        >  1:677826/1‑229 (MQ=255)                                                                                 aGCACTTAACCGAGAGGTGATTGAATACTTCAAAGAGCATGTCAATCCGGGGTTTTTAGAGTATCGCAAATCTG‑TTACCGCC‑GGCGGGGATTACGGAGCCGTAGAGTGGCAAGCGGGAAGTTTAAATACGCTTGTCGACACCCAG‑GACCAGGAGTTTAACGTCTGCCTGGGGGGGTTTGGCAATTTCCACGTGGGGCACCGTAATCCA‑GGTTGGGTTTCCCCCGTACACaaataaactg                                                                                                                                                   >  2:994547/1‑231 (MQ=255)                                                                                      ttAACCGAGAGGTGATTGAATACTTCAAAGAGCATGTCAATCCGGGGTTTTTAGAGTATCGCAAATCTG‑TTACCGCC‑GGCGGGGATTACGGAGCCGTAGAGTGGCAAGCGGGAAGTTTAAATACGCTTGTCGACACCCAG‑GGCCAGGAGTTTATCGACTGCCTGGGAGGTTTTGGAATTTTCAACGTGGGGCACCGTAATCCA‑GTTGTGGTTTCCGCCGTACAGAATCAACTTGCGaaa                                                                                                                                              >  1:14232/1‑239 (MQ=255)                                                                                             agagGTGATTGAATACTTCAAAGAGCATGTCAATCCGGGGTTTTTAGAGTATCGCAAATCTG‑TTACCGCC‑GGCGGGGATTACGGAGCCGTAGAGTGGCAAGCGGGAAGTTTAAATACGCTTGTCGACACCCAG‑GGCCAGGAGTTTATCGACTGCCTGGGAGGGTTTGGAATTTTCAACGTGGGGCACCGGAATCCA‑GTTGTGGTTTCCGCCGTGCAGAATCAATTTGCGAAACAAACGc                                                                                                                                       >  1:514351/1‑239 (MQ=255)                                                                                                  tGATTGAATCCTTCAAAGGGCATGTCAATCCGGGGTTTTTAGAGTATCGAAAATCTG‑TTACCGCC‑GGCGGGGATTACGGAGCCGTAGAGTGGCAAGCGGGAAGTTTAAATACGCTTGTCGACACCCAG‑GGCCAGGAGTTTATCGACTGCCTGGGAGGTTTTGGAATTTTCAACGTGGGGCACCGTAATCCA‑GTTGTGGTTTCCGCCGTACAGAATCAACTTGCGAAACAACCGCTGca                                                                                                                                   <  1:632901/238‑1 (MQ=255)                                                                                                  tGATTGAATACTTCAAAGAGCAGGTCAATCCGGGGTTTTTAGAGTATCAAAAATCTG‑TTACCGCC‑GGCGGGGATTACGGAGCCGTAGAGTGGCAAGCGGGAAGTTTAAATACGCTTGTCGACACCCAG‑GGCCAGGAGTTTATCGACTGCCTGGGAGGTTTTGGAATTTTCAACGTGGGGCACCGTAATCCA‑GTTGTGGTTTCCGCCGTACAGAATCAACTTGCGAAACAACCGCTGca                                                                                                                                   <  1:652114/238‑1 (MQ=255)                                                                                                  gtaTTGAATCCTTTAAAGGGAAGTTCAATCCGGGGTTTTTGGAGTTGCGAAAATCTT‑TCACCGCG‑GGGGGGGATTACGGGGCCGGAGAGTGGCAAGCGGGAAGTTTAAATACGCTTGTCGACACCCAG‑GGCCAGGAGTTTAGCGACTGCCTGGGAGGTTTTGGAATTTTCAACGTGGGGCACCGTAATCCA‑GTTGTGGTTTCCGCCGTACAGAATCAACTTGCGAAACAACCGCTGca                                                                                                                                   <  2:831797/236‑1 (MQ=255)                                                                                                  ggtttGAAAATTTAAAAGGGCATGTCACTCCGGGGTTTTTGGAGTATGGAAAATGTG‑TTACCGGC‑GGCGGGGATTACGGAGCCGTAGAGTGGCAAGCGGGAAGTTTAAATACGCTTGTCGACACCCAG‑GGCCAGGAGTTTATCGACTGCCTGGGAGGTTTTGGAATTTTCAACGTGGGGCACCGTAATCCA‑GTTGTGGTTTCCGCCGTACAGAATCAACTTGCGAAACAACCGCTGca                                                                                                                                   <  2:89655/235‑1 (MQ=255)                                                                                                    aTTGAATACTTCAAAGAGCATGTCAATCCGGGGTTTTTAGAGTATCGCAAATCTG‑TTACCGCC‑GGCGGGGATTACGGAGCCGTAGAGTGGCAAGCGGGAAGTTTAAATACGCTTGTCGACACCCAG‑GGCCAGGAGTTTATCGACTGCCTGGGAGGGTTTGGAATTTTCAACGTGGGGCACCGTAATCCA‑GTTGTGGTTTCCGCCGGACAGAATCAACTTGCGAAAAAACCGCtcaacga                                                                                                                                >  2:630303/1‑233 (MQ=255)                                                                                                    aTTGAATACTTCAAAGAGCATGTCAATCCGGGGTTTTTAGAGTATCGCAAATCTG‑TTACCGCC‑GGCGGGGATTACGGAGCCGTAGAGTGGCAAGCGGGAAGTTTAAATACGCTTGTCGACACCCAG‑GGCCAGGAGGTTATCGACTGCCTGGGAGGTTTTGGAATTTTCAACGTGGGGCACCGTAATCCA‑GTTTTGGTTTCCGCCTGACAGAATCAACTTTGGAACAAACCGCTCcac‑a                                                                                                                                >  1:750140/1‑238 (MQ=255)                                                                                                               cAAAGAGCATGTCAATCCGGGGTTTTTAGAGTATCGCAAATCTG‑TTACCGCC‑GGCGGGGATTACGGAGCCGTAGAGTGGCAAGCGGGAAGTTTAAATACGCTTGTCGACACCCAG‑GGCCAGGAGTTTATCGACTGCCTGGGAGGTTTTGGAATTTTCAACGTGGGGCACCGTAATCCA‑GGTTGGGTTTCCGCCGTCCAGAATCAACTTGGGAAAAAACC‑CCGCACAAGCCAgggacgc                                                                                                                     >  2:608516/1‑233 (MQ=255)                                                                                                               cAAAGAGCATGTCAATCCGGGGTTTTTAGAGTATCGCAAATCTG‑TTACCGCC‑GGCGGGGATTACGGAGCCGTAGAGTGGCAAGCGGGAAGTTTAAATACGCTTGTCGACACCCAG‑GGCCAGGAGTTTATCGACTGCCTGGGAGGGTTTGGAATTTTCAACGCGGGGCGCCGTAATCCA‑GTTGTGGTTTCCGCCGTACAGAACCAAATTGCGAAAAAACCCCTGCAC‑AACAAGGAgcgga                                                                                                                    >  2:1017147/1‑236 (MQ=255)                                                                                                                     cgatttgctcccGTGGTTTTTAGGGTA‑CGCGAAACAGTTTCCCGCC‑GGCGGGGATGGCGGCCCCGGAGAGGGGCAAGCGGGAAGTTTAAATCCGCTTGTCGACACCCAG‑GGCCAGGCGTTTATCGACTGCCTGGGAGGTTGTGGAATTTACAACGTGGGGCACCGTAATCCA‑GTTGTGGTTTCCGCCGTACAGAATCAACTTGCGAAACAACCGCTGCAC‑AGCCAGGAGCTGCTCGa                                                                                                                <  2:905121/227‑1 (MQ=255)                                                                                                                      cATGTCAATCCGGGGTTTTTAGAGTATCGCAAATCTG‑TTACCGCC‑GGCGGGGATTACGGAGCCGTAGAGTGGCAAGCGGGAAGTTTAAATACGCTTGTCGACACCCCG‑GGCCAGGGGTTTATCGCCTGCCTGGGAGGGTTTGGAATTTTCAACGTGGGGAACCGTAAACCA‑GTTCTGGTTTCCGCCGTCAAGAACCAACTTTCGGAAAAACCGCAGCAA‑AGCCAGGAGCCGCCCGAAcc                                                                                                             >  2:19431/1‑239 (MQ=255)                                                                                                                       aTGTCACTCCGGGGTTTTTAGAGTATCGCAAATCTG‑TTACCGCC‑GGCGGGGATTACGGAGCCGTAGAGTGGCAAGCGGGAAGTTTAAATACGCTTGTCGACACCCAG‑GGCCAGGAGTTTATCGACTGCCTGGGAGGTTTTGGAATTTTCAACGTTGGGCACCGTCATCCA‑TTTGTGGTTTCCGCCGTCCAGGATCAACTTTCGAACCAACCGCTGCAC‑AGCCAGGAGCTGCTCGGTCCg                                                                                                            >  1:237176/1‑239 (MQ=255)                                                                                                                       aTGTCAATCCGGGGTTTTTAGAGTATCGCAAATCTG‑TTACCGCC‑GGCGGGGATTACGGAGCCGTAGAGTGGCAAGCGGGAAGTTTAAATACGCTTGTCGACACCCAG‑GGCCAGGAGTTTATCGACTGCCTGGGCGGTTTTGGAATTTTCAACGCGGGGCACCGTAATCCA‑GTTTTGGTTTCCGCCGTACAGAATCCACTTGCGAAACAACCGCTGCAC‑AGCCCGGAGCTGCTCGATCCg                                                                                                            >  1:357770/1‑239 (MQ=255)                                                                                                                          tCAATCCGGGTTTTTTAGAATTTCGAAAATCGG‑TTACCCCC‑GGCGGGGATTGCGGAGCCGTAGAGTGGCAAGCGGGAAGTTTAAATTCGCTTGTCGACACCCAG‑GGCCGGGAGTTTATCGACTGCATGGGAGGTTTTGGAATTTTCAACGTGGGGCACCGTAATCCA‑GTTGTGGTTTCCGCCGTACAGAATCAACTTGCGAAACAACCGCTGCAC‑AGCCAGGAGCTGCTCGATCCGTTa                                                                                                         <  2:695452/239‑1 (MQ=255)                                                                                                                              tCCGGGGTTTTTAGAGTATCGCAAATCTG‑TTACCGCC‑GGCGGGGATTACGGAGCCGTAGAGTGGCAAGCGGGAAGTTTAAATACGCTTGTCGACACCCAG‑GGCCAGGAGTTTATCGACTGCCTGGGAGGTTTTGGAATTTTCAACGTGGGGCACCGTAATCCA‑GTTGTGGTTTCCGCCGTACAGAATCAACTTGCGAAAAAACCGCTGCAC‑AGCCAGGAGCTGCTCGATCCGTTACggg                                                                                                     >  1:5792/1‑239 (MQ=255)                                                                                                                              ggggggtTTTTTAGAGTTGCGGAAATCTG‑TT‑CCGCCGGGGGGGGATTCGGGGGCGGGAGGGGGGAAAGCGGGAAGTTTAAATACGCTTGTCGACACCCAG‑GGCCAGGAGTTTATCGACTGCCTGGGAGGTTTTGGAATTTGCAACGTGGGGCACCGTAATCCA‑GTTGTGGTTTCCGCCGTACAGAATCAACTTGCGAAACAACCGCTGCAC‑AGCCAGGAGCTGCTCGATCCGTTACggg                                                                                                     <  2:1062661/236‑1 (MQ=255)                                                                                                                                  gggTTTTTAGAGTATCGCAAATCTG‑TTACCGAC‑GGCGGGGCTTACGGAGCCGTAGAGTGGCAAGCGGGAAGTTTAAATACGCTTGTCGACACCCAG‑GGCCAGGAGTTTATCGACTGCCTGGGAGGTTTTGGAATTTTCAACGTGGGGTCCCGTAATCCATGTTGGGGTTT‑CGCCGGACAGAATCAACTTGCGAAACAACCCCTGCAC‑CGCCAGGACCCCCCCGATACATCCCGGGCGAt                                                                                                 >  2:370303/1‑239 (MQ=255)                                                                                                                                                     aattgg‑TTACCGCG‑GGCGGGGATTACGGGGCGTGAGAGTGGCAAGCGGGAATTTTAACTGCGCTGGGCGACACCCTG‑GGCCTGGAGTTTATTGACTGCCTGGGAGGTTTTGGAATTTTCAACGTGGGGGACCGTAATCCA‑GTTGTGGTTTCCGCCGTACAGAATCAACTTGCGAAACAACCGCTGCAC‑AGCCAGGAGCTGCTCGATCCGTTACGGGCGATGTTGGCGAAAACCCTtgct                                                                              <  2:346441/234‑1 (MQ=255)                                                                                                                                                      aTCTG‑TTACCGCC‑GGCGGGGATTACGGAGCCGTAGAGTGGCAAGCGGGAAGTTTAAATACGCTTGTCGACACCCAG‑GGCCAGGAGTTTATCGACTGCCTGGGAGGTTTTGGAATTTTCAACGTGGGGCACCGTAATCCA‑GTTGTGGTTTCCGCCGTa                                                                                                                                                                <  1:927278/157‑1 (MQ=255)                                                                                                                                                      aTCTG‑TTACCGCC‑GGCGGGGATTACGGAGCCGTAGAGTGGCAAGCGGGAAGTTTAAATACGCTTGTCGACACCCAG‑GGCCAGGAGTTTATCGACTGCCTGGGAGGTTTTGGAATTTTCAACGTGGGGCACCGTAATCCA‑GTTGTGGTTTCCGCCGTa                                                                                                                                                                >  2:927278/1‑157 (MQ=255)                                                                                                                                                          g‑TTACCGCC‑GGCGGGGATTACGGAGCCGTAGAGTGGCAAGCGGGAAGTTTAAATACGCTTGTCGACACCCAG‑GGCCAGGAGTTTATCGACTGCCTGGGAGGTTTTGGAATTTTCAACGTGGGGCACCGTAATCCA‑GTTGTGGTTTCCGCCGTACAGAATCAACTTGCGAAACAACCGCTGCAC‑AGCCAGGAGCCGCTCGATCCGTTACGGGCGATGTTGGCGAAAACCCTTGCTGCGCt                                                                         >  1:576339/1‑239 (MQ=255)                                                                                                                                                                       cggGGATTACGGAGCCGTAGAGTGGCAAGCGGGAAGTTTAAATACGCTTGTCGACACCCAG‑GGCCAGGAGTTTATCGACTGCCTGGGAGGTTTTGGAATTTTCAACGTGGGGCACCGTAATCCA‑GTTGTGGTTTCCGCCGTACAGAATCAACTTGCGAAACAACCGCTGCAC‑AGCCAGGAGCTGCTCGATCCGTTACGGGCGATGTTGGCGAAAACCCCTGCTTCGCTAACCCCCGCTa                                                              >  1:903244/1‑239 (MQ=255)                                                                                                                                                                        ggggTTTACGGGGCCGTAGAGTGGCAAGGGGGAAGTTTAAATACGGTTGTCGACACCCAG‑GGCCAGGAGTTTATCGACTGCCTGGGAGGTTTTGGAATTTTCAACGGGGGGCACCGTAATCCA‑GTTGTGGTTTCCGCCGTACAGAATCAACTTGCGAAACAACCGCTGCAC‑AGCCAGGAGCTGCTCGATCCGTTACGGGCGATGTTGGCGAAAACCCTTGCTGCGCTAACGCCCGGTa                                                              <  2:906072/238‑1 (MQ=255)                                                                                                                                                                        ggggGTTCCGGGGCCGTGGGGGTGCAAGGGGGAAGTTTAAATACCTGTGGGGACACCCAG‑GGCCAGGAGTTTATCGACTGCCTGGGAGGTTTTGGAATTTTCAACGTGGGGCCCCGTAATCCA‑GTTGTGGTTTCCGCCGTACAGAATCAACTTGCGAAACAACCGCTGCAC‑AGCCAGGAGCTGCTCGATCCGTTACGGGCGATGTTGGCGAAAACCCTTGCTGCGCTAACGCCCGGTa                                                              <  2:690724/238‑1 (MQ=255)                                                                                                                                                                                cGGGGCCGTGGGGTGGAAAGGGGGAAGTTTAAATCCGCTTGTGGACCCCCGG‑GGCCGGGAGTTTTTGGACTGCCTGGGAGGTTTTGGAATTTTCAACGTGGGGCCCCGTAATCCA‑GTTGTGGTTTCCGCCGTACAGAATCAACTTGCGAAACAACCGCTGCAC‑AGCCAGGAGCTGCTCGATCCGTTACGGGCGATGTTGGCGAAAACCCTTGCTGCGCTAACGCCCGGTAAACTGAAAt                                                     <  2:14232/239‑1 (MQ=255)                                                                                                                                                                                cGGAGCCGTAGAGTAGCAAGCGGGAAGTTTAAATACGCTTGTCGACACCCAG‑GGCCAGGAGTTTATCGACTGCCTGGGAGGTTTTGGAATTTTCAACGTGGGGCACCGTAATCCA‑GTTGTGGTTTCCGCCGTACAGAATCAACTTGCGAAACAACCGCTGCAC‑AGCCAGGAGCTGCTCGATCCGTTACGGGCGATGTTGGCGAAAACCCTTGCTGCGCTAACGCCCGGTAAACTGAAAt                                                     <  1:240862/239‑1 (MQ=255)                                                                                                                                                                                    gccggtgggTGGCAAGCGGGAAGTTTAAATGCGGTTGTCGACACCCCG‑GGCCAGGAGTTTATCGACTGCCTGGGAGGTTTTGGAATTTTCAACGTGGGGCACCGTAATCCA‑GTTGTGGTTTCCGCCGTACAGAACCAACTTGCGAAACAACCGCTGCAC‑AGCCAGGAGCTGCTCGATCCGTTACGGGCGATGTTGGCGAAAACCCTTGCTGCGCTAACGCCCGGTAAACTGAAATACa                                                  <  2:500999/230‑1 (MQ=255)                                                                                                                                                                                    gCCGTAGAGTGGCAAGCGGGAAGTTTAAATACGCTTGTCGACACCCAG‑GGCCAGGAGTTTATCGACTGCCTGGGAGGTTTTGGAATTTTCAACGTGGGGCACCGTAATCCA‑GTTGTGGTTTCCGCCGTACAGACTAAACTTGCGAAACAACCGCTGCAC‑AGCCAGGCGCTGCTCGATCCGTTACGGGCGACGGTTGGGGAAACCCTTGGCGCGCTAACGCCCGGAAAAGTGaaaaaaa                                                  >  2:861578/1‑234 (MQ=255)                                                                                                                                                                                      gttggggTGGAAAGGGGAAATTTTAAACCCGCTTGTCGCCGCCCAG‑GGCCAGGAGTTTATCGACTGTCTGGGGGGTTTTGGAATTGTCAACGTGGGGCCCCGTAACCCA‑GTTGGGGTTTCCGCCGTACAGAATCAACTTGCGAAACAACCGCGGCAC‑AGCCAGGAGCTGCTCGATCCGTTACGGGCGATGTTGGCGAAAACCCTTGCTGCGCTAACGCCCGGTAAACTGAAATACAGct                                               <  2:699036/233‑1 (MQ=255)                                                                                                                                                                                        tAGAGTGGCAAGCGGGAAGTTTAAATACGCTTGTCGACACCCAG‑GGCCAGGAGTTTATCGACTGCCTGGGAGGTTTTGGAATTTTCAACGTGGGGCACCGTAATCCA‑GTTGTGGTTTCCGCCGTACAGAATCAACTTGCGAAACAACCGCTGCAC‑AGCCAGGACCGGCCCGATCCGTTACGGGCGACGTTTGCGAAAACCCTTGCTGCGCAAACCACCGGTAAACTGAAATAAAggt                                               >  2:463062/1‑235 (MQ=255)                                                                                                                                                                                         gggggggCAAGGGGGAATTTTTAATTCCTTTGTCGGCCCCCAG‑GGCCGGGAGTTTAGCGACTGCCTGGGAGGTTTTGGAATTTTCAACGGGGGGAACCGTAATCCA‑GTTGTGGTTTCCGCCGTACAGAATCAACTTGCGAAACAACCGCTGCAC‑AGCCAGGAGCTGCTCGATCCGTTACGGGCGATGTTGGCGAAAACCCTTGCTGCGCTAACGCCCGGTAAACTGAAATACAGcttct                                            <  2:514351/234‑1 (MQ=255)                                                                                                                                                                                           ggttgCAAGCGGGAAGTTTAAAGCCGCTTTTCGAACCCCAG‑GGCCAGGGGTTTATCGACTGTCTGGGAGGTTTTGGAATTTTCAACGTGGGGCACCGTAATCCA‑GTTGTGGTTTCCGCCGTACAGAATCAACTTGCGAAACAACCGCTGCAC‑AGCCAGGAGCTGCTCGATCCGTTACGGGCGATGTTGGCGAAAACCCTTGCTGCGCTAACGCCCGGTAAACTGAAATACAGcttctt                                           <  2:5792/234‑1 (MQ=255)                                                                                                                                                                                               gCAAGCGGGAAGTTTAAATACGCTTGTCGACACCCAG‑GGCCAGGAGTTTATCGACTGCCTGGGAGGTTTTGGAATTTTCAACGTGGGGCACCGTAATCCA‑GTTGTGGTTTCCGCCGTACAGAATCAACTTGCGAAACAACCGCTGCAC‑AGCCGAGAGCTGCCCGATCCGTTACGGGCGGTGTTGGGGAAAACCCTTGCTGCGGCAACGCCCGGGAAACCGAAATAAACCTTCTTCTGTa                                      >  2:37211/1‑239 (MQ=255)                                                                                                                                                                                               gCAAGCGGGAAGTTTAAATACGCTTGTCGACACCCAG‑GGCCAGGAGTTTATCGACTGCCTGGGAGGTTTTGGAATTTTCAACGTGGGGCACCGTAATCCA‑GTTGTGGTTTCCGCCGTACAGAATCAACTTGCGAAACAACCGCTGCAC‑AGCCAGGAGCTGCTCGATCCGTTACGGGCGATGTTGGCGAAAACCCTTGCTGCGCTAACGCCCGGTAAACTGAAATACAGCTCCTTCTGTa                                      >  1:317936/1‑239 (MQ=255)                                                                                                                                                                                               gCAAGCGGGAAGTTTAAATACGCTTGTCGACACCCAG‑GGCCAGGAGTTTATCGACTGCCTGGGAGGTTTTGGAATTTTCAACGTGGGGCACCGTAATCCA‑GTTGTGGTTTCCGCCGTACAGAATCAACTTGCGAAACAACCGCTGCAC‑AGCCAGGAGCTGCTCGATCCGTTACGGGCGATGTTGGCGAAAACCCTTGCTGCCCTAACGCCCGGTAAACTGAAATCCAGCTTCTTCTGTa                                      >  1:42918/1‑239 (MQ=255)                                                                                                                                                                                               gCAAGCGGGAAGTTTAAATACGCTTGTCGACACCCAG‑GGCCAGGAGTTTATCGACTGCCTGGGAGGTTTTGGAATTTTCAACGTGGGGCACCGTAATCCA‑GTTGTGGTTTCCGCCGTACAGAAACAACTTGCGAAACAACCGCTGCAC‑AGCCAGGAGCTGCTCGATCCGTTACGGGCGATGTTGGCGAAAACCCTTGCTGCGCTAACGCCCGGTAAACTGAAATACACACtcttcttga                                      >  1:778295/1‑236 (MQ=255)                                                                                                                                                                                                cAAGTGGGGAGTTTAAATTCTGTTTGCTGCAACCAGAGGCCAGGATTTGTACGACTGCCTGGGAGGTTGTGGAATTTTCAAGGTGGGGCACCGTAACCCG‑GTTGTGTTTTCCACCGTACAGAATCAACTTGCGAAACAACCGCTGCAC‑AGCCAGGAGCTGCTCGATCCGTTACGGGCGATGTTGGCGAAAACCCTTGCTGCGCTAACGCCCGGTAAACTGAAATACAGCTTCTTCTGt                                       <  2:239037/238‑1 (MQ=255)                                                                                                                                                                                                 aaGCGGTAAGTTTAAATTCCCTTTGCGACCCCCAG‑GGCCAGGATTTTATCGACTGCCTGGGAGGTTTTGGAATTGTCAACGGGGGGCACCTTAATCCA‑GTTGTGGTTTCCGCCGTACAGAATCAACTTGCGAAACAACCGCTGCAC‑AGCCAGGAGCTGCTCGATCCGTTACGGGCGATGTTGGCGAAAACCCTTGCTGCGCTAACGCCCGGTAAACTGAAATACAGCTTCTTCTGTaa                                     <  2:1088405/238‑1 (MQ=255)                                                                                                                                                                                                  aGCGGGAAGTTTAAATACGCTTGTCGACACCCAG‑GGCCAGGAGTTTATCGACTGCCTGGGAGGTTTTGGAATTTTCAACGTGGGGCACCGTAATCCA‑GTTGTGGTTTCCGCCGTACAGAGTCAACTTGCGAAACAACCGCTGCAC‑AGCCAGGAGCTGCCCGATCCGTTACGGGCGATGTTGGCGAAACCCCTT‑CGGCGCCAAAGCCCGGGAAACTGAAATACAGCCTCTTCTGTAATaa                                  >  2:1062323/1‑238 (MQ=255)                                                                                                                                                                                                  aGCGGGAAGTTTAAATACGCTTGTCGACACCCAG‑GGCCAGGAGTTTATCGACTGCCTGGGAGGTTTTGGAATTTTCAACGTGGGGCACCGTAATCCA‑GTTGTGGTTTCCGCCGTACAGAATCAACTTGCGAAACAACCGCTGCCC‑AGCCAGGAGCTGCTCGATCCGTTACTGGCGATGTTGGCGAAAACCCTTGCTGCGCTAACTCCCGGTAAAAGGAAATAAAGCTGCTTCTTTaaaa                                   >  2:768533/1‑237 (MQ=255)                                                                                                                                                                                                  aGCGGGAAGTTTAAATACGCTTGTCGACACCCAG‑GGCCAGGAGTTTATCGACTGCCTGGGAGGTTTTGGAATTTTCAACGTGGGGCACCGTAATCCA‑GTTGTGGTTTCCGCCGTACAGAATCAACTTGCGAAACAACCGCTGCAC‑AGCCAGGAGCTGCTCGATCCGTTACGGGCGATGTTGGCGCAAACCCTTGCTGCGCTAACGCCCGGTAAACTGAAATACAGCTTCTTCTGTAATa                                   >  1:253663/1‑239 (MQ=255)                                                                                                                                                                                                  aGCGGGAAGTTTAAATACGCTTGTCGACACCCAG‑GGCCAGGAGTTTATCGACTGCCTGGGAGGTTTTGGAATTTTCAACGTGGGGCACCGTAATCCA‑GTTGTGGTTTCCGCCGTACAGAATCAACTTGCGAAACAACCGCTGCAC‑AGCCAGGAGCTGCTCGATCCGTTACGGGCGATGTTGGCGAAAACCCTTTCTGCGCTAACGCCCGGTAAACTGAAAAACAGCTCTTTTTGTAATa                                   >  1:96204/1‑239 (MQ=255)                                                                                                                                                                                                  aGCGGGAAGTTTAAATACGCTTGTCGACACCCAG‑GGCCAGGAGTTTATCGACTGCCTGGGAGGTTTTGGAATTTTCAACGTGGGGCACCGTAATCCA‑GTTGTGGTTTCCGCCGTACAGAATCAACTTGCGAAACAACCGCTGCAC‑AGCCAGGAGCTGCTCGATCCGTTACGGGCGATGTTGGCGAAAACCCTTGCTGCGCTAACGCCCGGTAAACTGAAATACAGCTTCTTCTGTAATa                                   >  1:13834/1‑239 (MQ=255)                                                                                                                                                                                                           tttAAATCCGCTTGTCGACCCCCAG‑GGCCTGGGGTTTAACGCCTGCCTTGGAGGTTTTGGAATTTTCAACGTGGGGCACCGTAATCCA‑GTTGTGGTTTCCGCCGTACAGAATCAACTTGCGAAACAACCGCTGCAC‑AGCCAGGAGCTGCTCGATCCGTTACGGGCGATGTTGGCGAAAACCCTTGCTGCGCTAACGCCCGGTAAACTGAAATACAGCTTCTTCTGTAATAGCGGCAcc                           <  2:70582/238‑1 (MQ=255)                                                                                                                                                                                                                  ccGCTTGTCGACACCCAG‑GGCCAGGAGTTTATCGACTGCCTGGGAGGTTTTGGAATTTTCAACGTGGGGCACCGTAATCCA‑GTTGTGGTTTCCGCCGTACAGAATCAACTTGCGAAACAACCGCTGCAC‑AGCCAGGAGCTGCTCGATCCGTTACGGGCGATGTTGGCGAAAACCCTTGCTGCGCTAACGCCCGGTAAACTGAAATACAGCTTCTTCTGTAATAGCGGCACCGAGTCCGt                   <  1:895373/238‑1 (MQ=255)                                                                                                                                                                                                                     gttttgaaaccccAG‑GGGCGGGGTTTTTCCGACTGCCGGGGGGGTTTGGGAATTTTCAACGGGGGGCACCGTAATCCA‑GTTGGGGTTTCCGCCGTACAGAATCAACTTGCGAAACAACCGCTGCAC‑AGCCAGGAGCTGCTCGATCCGTTACGGGCGATGTTGGCGAAAACCCTTGCTGCGCTAACGCCCGGTAAACTGAAATACAGCTTCTTCTGTAATAGCGGCACCGAGTCCGTTGa                <  2:357770/229‑1 (MQ=255)                                                                                                                                                                                                                     cTTGTCGACACCCAG‑GGCCAGGAGTTTATCGACTGCCTGGGAGGTTTTGGAATTTTCAACGTGGGGCACCGTAATCCA‑GTTGTGGTTTCCGCCGTACAGAATCAACTTGCGAAACAACCGCTGCAC‑AGCCAGGAGCTGCTCGATCCGTTACGGGCGATGTTGGCGAAAACCCTTGCTGCGCTAACGCCCGGTAAACTGAAATACAGCTTCTTCTGTAATAGCGGCACCGGGTCCGTTg                 >  1:70582/1‑238 (MQ=255)                                                                                                                                                                                                                       tttCGACACCCGG‑GGCCGGGGGTTTATCGATGGCCTGGGAGTTTTGGGAATTTTCAACGGGGGGCACCGTAATCCA‑GTTGTGGTTTCCGCCGTACAGAATCAACTGGCGAAACAACCGCTGCAC‑AGCCAGGAGCTGCTCGATCCGTTACGGGCGATGTTGGCGAAAACCCTTGCTGCGCTAACGCCCGGTAAACTGAAATACAGCTTCTTCTGTAATAGCGGCACCGAGTCCGTTGAag              <  2:562286/237‑1 (MQ=255)                                                                                                                                                                                                                       tggcgtcccccAG‑GGCCAGGAGTTTAGCGACTGCCTGGGAGGTTTTGGAATTTTCAACGTGGGGCACCGTAATCCA‑GTTGTGGTTTCCGCCGTACAGAATCAACTTGCGAAACAACCGCTGCAC‑AGCCAGGAGCTGCTCGATCCGTTACGGGCGATGTTGGCGAAAACCCTTGCTGCGCTAACGCCCGGTAAACTGAAATACAGCTTCTTCTGTAATAGCGGCACCGAGTCCGTTGAag              <  1:630303/231‑1 (MQ=255)                                                                                                                                                                                                                                  aG‑GGCCAGGAGTTTATCGACTGCCTGGGAGGTTTTGGAATTTTCAACGTGGGGCACCGTAATCCA‑GTTGTGGTTTCCGCCGTACAGAATCAACTTGCGAAACAACCGCTGCAC‑AGCCAGGCGCTGCTCGATCCGTTACGGGCGCTGTTGGCGAAAACCCTTGCTGCGCTAACCCCCGGTAAACTGAAATACAGCTTCTTCTTTAATCACGGCACCGACTCCGTGGAAACAGCGCCGaa    >  2:755342/1‑238 (MQ=255)                                                                                                                                                                                                                                  aG‑GGCCAGGAGTTTATCGACTGCCTGGGAGGTTTTGGAATTTTCAACGTGGGGCACCGTAATCCA‑GTTGTGGTTTCCGCCGTACAGAATCAACTTGCGAAACAACCGCTGCAC‑AGCCAGGAGCTGCTCGATCCGTTACGGGCGCTGTTGGCGAAAACCCTTGCTGCGCTAACGCCCGGTAAACTGAAATACCGCTTCTTCTGGAATAGCGGCACCGAGTCCGTTGCAGCAGCGCTGaa    >  1:248571/1‑238 (MQ=255)                                                                                                                                                                                                                                  aG‑GGCCAGGAGTTTATCGACTGCCTGGGAGGTTTTGGAATTTTCAACGTGGGGCACCGTAATCCA‑GTTGTGGTTTCCGCCGTACAGAATCAACTTGCGAAACAACCGCTGCAC‑AGCCAGGAGCTGCTCGATCCGTTACGGGCGATGTTGGCGAAAACCCTTGCTGCGCTAACGCCCGGTAAACTGAAATACAGCTTCTTCTGTAATAGCGGCACCGAGGCCGTTGAAGCAACGCGGaa    >  2:342156/1‑238 (MQ=255)                                                                                                                                                                                                                                   g‑ggCCAGGAGTTTATCGACTGCCTGGGAGGTTTTGGAATTTTCAACGTGGGGCACCGTAATCCA‑GTTGTGGTTTCCGCCGTACAGAATCAACTTGCGAAACAACCGCTGCAC‑AGCCAGGAGCTGCTCGATCCGTTACGGGCGATGTTGGCGAAAACCCTTGCTGCGCTAACGCCCGGTAAACTGAAATACAGCTTCTTCTGTAATAGCGGCACCGAGTCCGTTGAAGCAGCGCTGAAGc  >  1:972352/1‑239 (MQ=255)                                                                                                                                                                                                                                      gCCAGGAGTTTATCGACTGGCTGGGAGGTTTTGGAATTTTCAACGTGGGGCACCGTAATCCA‑GTTGTGGTTTCCGCCGTACAGAATCAACTTGCGAAACAACCGCTGCAC‑AGCCAGGAGCTGCTCGATCCGTTACGGGCGATGTTGGCGAAAACCCTTGCTGCGCTAACGCCCGGTAAACTGAAATACAGCTTCTTCTGTAATAGCGGCACCGAGTCCGTTGAAGCAGCGCTGAAg   <  1:19431/236‑1 (MQ=255)                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                         TCGAGCGCATCGGCTTTAGCGTGCAGCGCCCACGCCCTGAATCTCATTGAGAAGCGAACGCTGGATCATGAGGAGATGAAAGCACTTAACCGAGAGGTGATTGAATACTTCAAAGAGCATGTCAATCCGGGGTTTTTAGAGTATCGCAAATCTG‑TTACCGCC‑GGCGGGGATTACGGAGCCGTAGAGTGGCAAGCGGGAAGTTTAAATACGCTTGTCGACACCCAG‑GGCCAGGAGTTTATCGACTGCCTGGGAGGTTTTGGAATTTTCAACGTGGGGCACCGTAATCCA‑GTTGTGGTTTCCGCCGTACAGAATCAACTTGCGAAACAACCGCTGCAC‑AGCCAGGAGCTGCTCGATCCGTTACGGGCGATGTTGGCGAAAACCCTTGCTGCGCTAACGCCCGGTAAACTGAAATACAGCTTCTTCTGTAATAGCGGCACCGAGTCCGTTGAAGCAGCGCTGAAGC  >  NC_000913/3219509‑3219971

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑