| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 1,164,976 | G→A | 6.1% | P294P (CCG→CCA) | nagZ → | beta N‑acetyl‑glucosaminidase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,164,976 | 0 | G | A | 6.1% | 211.0 / 3.0 | 65 | P294P (CCG→CCA) | nagZ | beta N‑acetyl‑glucosaminidase |
| Reads supporting (aligned to +/- strand): ref base G (28/33); new base A (2/2); total (30/35) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.82e-01 | |||||||||||
CCTTAATCCGCGAAAATAAACTCGACGCCATTATGCCTGCGCATGTGATCTACAGTGATGTTGATCCGCGTCCGGCGAGCGGTTCTCCCTACTGGCTGAAAACCGTTTTGCGTCAGGAACTGGGTTTTGACGGCGTGATTTTCTCTGACGATTTATCGATGGAAGGTGCCGCGATTATGGGCAGTTATGCCGAACGCGGGCAGGCTTCACTGGATGCGGGTTGCGATATGATCCTGGTCTGCAATAATCGTAAAGGGGCCGTCAGCGTGTTAGATAATCTGTCACCGATCAAGGCAGAACGTGTTACACGTTTGTATCATAAAGGTTCATTTTCGCGACAGGAACTGATGGACTCGGCTCGCTGGAAAGCGATCAGCACCCGTCTGAATCAGTTACATGAACGCTGGCAGGAAGAGAAAGCAGGTCACTAACCCTGGCTTATGTGAGGAAGCGATGATTATCTATTTACACGGTTTTGACTCTAACAGTCCGGGTAACCACGAGAAAGTCTTACAATTGCAGTTTATTGACCCGGATGTACGCTTGATAAGCTACAGTACGCGGCATCCGAA > NC_000913/1164690‑1165261 | ccTTAATCCGCGAAAATAAACTCGACGCCATTGTGCCTGCGCATGTGATCTACAGTGATGTTGATCCGCGTCCGGCGAGCGGTTCTCCCTACTGGCTGAAAACCGTTTTGCGTCAGGAACTGGGTTTTGACGGCGTGATTTTCTCTGACGATTTATCGATGGAAGGTGCCGCGATTATGGGCAGTTATGCCGAACGCGGGCAGGCTTCACTGGATGCGGGTTGCGATATGATCCTGGTCTGCAATAATCGTAAAGGGGCCGTCAGCGTGTTAGATAATCTGTCACCGAt < 2:422477/289‑1 (MQ=255)ccTTAATCCGCGAAAATAAACTCGACGCCATTATGCCTGCGCATGTGATCTACAGTGATGTTGATCCGCGTCCGGCGAGCGGTTCTCCCTACTGGCTGAAAACCGTTTTGCGTCAGGAACTGGGTGTTGACGGCGTGATTTTCTCTGACGATTTATCGATGGAAGGTGCCGCGATTATGGGCAGTTATGCCGAACGCGGGCAGGCTTCACTGGATGCGGGTTGCGATATGATCCTGGTCTGCAATAATCGTAAAGGGGCCGTCAGCGTGTTAGATAATCTGTCACCGAt < 2:61849/289‑1 (MQ=255) cTTAATCCGCGAAAATAAACTCGACGCCATTATGCCTGCGCATGTGATCTACAGTGATGTTGATCCGCGTCCGGCGAGCGGTTCTCCCTACTGGCTGAAAACCGTTTTGCGTCAGGAACTGGGTTTTGACGGCGTGATTTTCTCTGACGATTTATCGATGGAAGGTGCCGCGATTATGGGCAGTTATGCCGAACGCGGGCAGGCTTCACTGGATGCGGGTTGCGATATGATCCTGGTCTGCAATAATCGTAAAGGGGCCGTCAGCGTGTTAGATAATCTGTCACCGATc < 2:386329/289‑1 (MQ=255) agccGCGAAAATAAACTGAGCGCCATTATGCCTGGGAATGGTATCTACAGTGATGTTGATCCGCGTCCTGCGCGCGGTTCTCCCTACTGGCTGAAAACCGTTTTGCGTCAGGAACTGGGTTTTGACGGCGTGTTTTTCTCTGACGATTTATCGATGGAAGGTGCCGCGATTATGGGCAGTTATGCCGAACGCGGGCAGGCTTCACTGGATGCGGGTTGCGATATGATCCTAGTCTGCAATAATCGTAAAGGGGCCGTCAGCGTGTTAGATAATCTGTCACCGATCAAgg < 2:443142/287‑1 (MQ=255) gAAAATAAACTCGACGCCATTATGCCTGCGCATGTGATCTACAGTGATGTTGATCCGCGTCCGGCGAGCGGTTCTCCCTACTGGCTGAAAACCGTTTTGCGTCAGGAACTGGGTTTTGACGGCGTGATTTTCTCTGACGATTTATCGATGGAAGGTGCCGCGATTATGGGCAGTTATGCCGAACGCGGGCAGGCTTCACTGGATGCGGGTTGCGATATGATCCTGGTCTGCAATAATCGTAAAGGGGCCGTCAGCGTGTTAGATAATCTGTCACCGATCAAGGCAGAAc > 1:438997/1‑289 (MQ=255) aaaCTCGACGCCATTATGCCTGCGCATGTGATCTACAGTGATGTTGATCCGCGTCCGGCGAGCGGTTCTCCCTACTGGCTGAAAACCGTTTTGCGTCAGGAACTGGGTTTTGACGGCGTGATTTTCTCTGACGATTTATCGATGGAAGGTGCCGCGATTATGGGCAGTTATGCCGAACGCGGGCAGGCTTCACTGGATGCGGGTTGCGATATGATCCTGGTCTGCAATAATCGTAAAGGGGCCGTCAGCGTGTTAGATAATCTGTCACCGATCAAGGCAGAACGTGTTa < 1:359808/289‑1 (MQ=255) aCTCGACGCCATTATGCCTGCGCATGTGATCTACAGTGATGTTGATCCGCGTCCGGCGAGCGGTTCTCCCTACTGGCTGAAAACCGTTTTGCGTCAGGAACTGGGTTTTGACGGCGTGATTTTCTCTGACGATTTATCGATGGAAGGTGCCGCGATTATGGGCAGTTATGCCGAACGCGGGCAGGCTTCACTGGATGCGGGTTGCGATATGATCCTGGTCTGCAATAATCGTAAAGGGGCCGTCAGCGTGTTAGATAATCTGTCACCGATCAAGGCAGAACGTGTTaca < 2:438997/289‑1 (MQ=255) cGACGCCATTATGCCTGCGCATGTGATCTACAGTGATGTTGATCCGCGTCCGGCGAGCGGTTCTCCCTACTGGCTGAAAACCGTTTTGCGTCAGGAACTGGGTTTTGACGGCGTGATTTTCTCTGACGATTTATCGATGGAAGGTGCCGCGATTATGGGCAGTTATGCCGAACGCGGGCAGGCTTCACTGGATGCGGGTTGCGATATGATCCTGGTCTGCAATAATCGTAAAGGGGCCGTCAGCGTGTTAGATAATCTGTCACCGATCAAGGCAGAACGTGTTACACGt < 2:202405/289‑1 (MQ=255) gcgcATGTGATCTACAGTGATGTTGATCCGCGTCCGGCGAGCGGTTCTCCCTACTGGCTGAAAACCGTTTTGCGTCAGGAACTGGGTTTTGACGGCGTGATTTTCTCTGACGATTTATCGATGGAAGGTGCCGCGATTATGGGCAGTTATGCCGAACGCGGGCAGGCTTCACTGGATGCGGGTTGCGATATGATCCTGGTCTGCAATAATCGTAAAGGGGCCGTCAGCGTGTTAGATAATCTGTCACCGATCAAGGCAGAACGTGTTACACGTTTGTATCATAAAGttt > 2:322688/1‑289 (MQ=255) cgcATGTGATCTACAGTGATGTTGATCCGCGTCCGGCGAGCGGTTCTCCCTACTGGCTGAAAACCGTTTTGCGTCAGGAACTGGGTTTTGACGGCGTGATTTTCTCTGACGATTTATCGATGGAAGGTGCCGCGATTATGGGCAGTTATGCCGAACGCGGGCAGGCTTCACTGGATGCGGGTTGCGATATGATCCTGGTCTGCAATAATCGTAAAGGGGCCGTCAGCGTGTTAGATAATCTGTCACCGATCAAGGCAGAACGTGTTACACGTTTGTCTCATAAAGGTTc > 2:398106/1‑289 (MQ=255) gATCTACAGTGATGTTGATCCGCGTCCGGCGAGCGGTTCTCCCTACTGGCTGAAAACCGTTTTGCGTCAGGAACTGGGTTTTGACGGCGTGATTTTCTCTGACGATTTATCGATGGAAGGTGCCGCGATTATGGGCAGTTATGCCGAACGCGGGCAGGCTTCACTGGATGCGGGTTGCGATATGATCCTGGTCTGCAATAATCGTAAAGGGGCCGTCAGCGTGTTAGATAATCTGTCACCGATCAAGGCAGAACGTGTTACACGTTTGTATCATAAAGGTTCATTTTc < 1:150388/288‑1 (MQ=255) gTGATGTTGATCCGCGTCCGGCGAGCGGTTCTCCCTACTGGCTGAAAACCGTTTTGCGTCAGGAACTGGGTTTTGACGGCGTGATTTTCTCTGACGATTTATCGATGGAAGGTGCCGCGATTATGGGCAGTTATGCCGAACGCGGGCAGGCTTCACTGGATGCGGGTTGCGATATGATCCTGGTCTGCAATAATCGTAAAGGGGCCGTCAGCGTGTTAGATAATCTGTCACCGATCAAGGCAGAACGTGTTa > 2:6059/1‑252 (MQ=255) gTGATGTTGATCCGCGTCCGGCGAGCGGTTCTCCCTACTGGCTGAAAACCGTTTTGCGTCAGGAACTGGGTTTTGACGGCGTGATTTTCTCTGACGATTTATCGATGGAAGGTGCCGCGATTATGGGCAGTTATGCCGAACGCGGGCAGGCTTCACTGGATGCGGGTTGCGATATGATCCTGGTCTGCAATAATCGTAAAGGGGCCGTCAGCGTGTTAGATAATCTGTCACCGATCAAGGCAGAACGTGTTa < 1:6059/252‑1 (MQ=255) gTGATGTTGATCCGCGTCCGGCGAGCGGTTCTCCCTACTGGCTGAAAACCGTTTTGCGTCAGGAACTGGGTTTTGACGGCGTGATTTTCTCTGACGATTTATCGATGGAAGGTGCCGCGATTATGGGCAGTTATGCCGAACGCGGGCAGGCTTCACTGGATGCGGGTTGCGATATGATCCTGGTCTGCAATAATCGTAAAGGGGCCGTCAGCGTGTTAGATAATCTGTCACCAATCAAGGCAGAACGTGTTACACGTTTGTATCATAAAGGTTCATTTTCGCGACAGGa > 1:180895/1‑289 (MQ=255) gTGATGTTGATCCGCGTCCGGCGAGCGGTTCTCCCTACTGGCTGAAAACCGTTTTGCGTCAGGAACTGGGTTTTGACGGCGTGATTTTCTCTGACGATTTATCGATGGAAGGTGCCGCGATTATGGGCAGTTATGCCGAACGCGGGCAGGCTTCACTGGATGCGGGTTGCGATATGATCCTGGTCTGCAATAATCGTAAAGGGGAAGTCATCGTGTTAGATAATCTGTCACCGATCAAGGCAGAACGTGTTACACGTTTGTATCATAAAGGTTCATTTTCGCGACAGGa > 1:260649/1‑289 (MQ=255) aTGTTGATCCGCGTCCGGCGAGCGGTTCTCCCTACTGGCTGAAAACCGTTTTGCGTCAGGAACTGGGTTTTGACGGCGTGATTTTCTCTGACGATTTATCGATGGAAGGTGCCGCGATTATGGGCAGTTATGCCGAACGCGGGCAGGCTTCACTGGATGCGGGTTGCGATATGATCCTGGTCTGCAATAATCGTAAAGGGGCCGTCAGCGTGTTAGATAATCTGTCACCGATCAAGGCAGAACGTGTTACACGTTTGTATCATAAAGGTTCATTTTCGCGACAGGa > 2:10603/1‑286 (MQ=255) ttGATCCGCGTCCGGCGAGCGGTTCTCCCTACTGGCTGAAAACCGTTTTGCGTCAGGAACTGGGTTTTGACGGCGTGATTTTCTCTGACGATTTATCGATGGAAGGTGCCGCGATTATGGGCAGTTATGCCGAACGCGGGCAGGCTTCACTGGATGCGGGTTGCGATATGATCCTGGTCTGCAATAATCGTAAAGGGGCCGTCAGCGTGTTAGATAATCTGTCACCGAt < 2:208601/229‑1 (MQ=255) ttGATCCGCGTCCGGCGAGCGGTTCTCCCTACTGGCTGAAAACCGTTTTGCGTCAGGAACTGGGTTTTGACGGCGTGATTTTCTCTGACGATTTATCGATGGAAGGTGCCGCGATTATGGGCAGTTATGCCGAACGCGGGCAGGCTTCACTGGATGCGGGTTGCGATATGATCCTGGTCTGCAATAATCGTAAAGGGGCCGTCAGCGTGTTAGATAATCTGTCACCGAt > 1:208601/1‑229 (MQ=255) ttGATCCGCGTCCGGCGAGCGGTTCTCCCTACTGGCTGAAAACCGTTTTGCGTCAGGAACTGGGTTTTGACGGCGTGATTTTCTCTGACGATTTATCGATGGAAGGTGCCGCGATTATGGGCAGTTATGCCGAACGCGGGCAGGCTTCACTGGATGCGGGTTGCGATATGATCCTGGTCTGCAATAATCGTAAAGGGGCCGTCAGCGTGTTAGATAATCTGTCACCGATCAAGGCAGAACGTGTTACACGTTTGTATCATAAAGGTTCATTTTCGCGACAGGAACTGAt > 1:31697/1‑289 (MQ=255) gATCCGCGTCCGGCGAGCGGTTCTCCCTACTGGCTGAAAACCGTTTTGCGTCAGGAACTGGGTTTTGACGGCGTGATTTTCTCTGACGATTTATCGATGGAAGGTGCCGCGATTATGGGCAGTTATGCCGAACGCGGGCAGGCTTCACTGGATGCGGGTTGCGATATGATCCTGGTCTGCAATAATCGTAAAGGGGCCGTCAGCGTGTTAGATAATCTGTCACCGATCAAGGCAGAACGTGTTACACGTTGGTATCATAAAGGTTCATTTTCGCGACAGGAACTGATgg > 1:439531/1‑289 (MQ=255) tCCGCGTCCGGCGAGCGGTTCTCCCTACTGGCTGAAAACCGTTTTGCGTCAGGAACTGGGTTTTGACGGCGTGATTTTCTCTGACGATTTATCGATGGAAGGTGCCGCGATTATGGGCAGTTATGCCGAACGCGGGCAGGCTTCACTGGATGCGGGTTGCGATATGATCCTGGTCTGCAATAATCGTAAAGGGGCCGTCAGCGTGTTAGATAATCTGTCACCAATCAAGGCAGAACGTGTTACACGTTTGTATCATAAAGGTTCATTTTCGCGACAGGAACTGATGGAc < 2:180895/289‑1 (MQ=255) gcgTCCGGCGAGCGGTTCTCCCTACTGGCTGAAAACCGTTTTGCGTCAGGAACTGGGTTTTGACGGCGTGATTTTCTCTGACGATTTATCGATGGAAGGTGCCGCGATTATGGGCAGTTATGCCGAACGCGGGCAGGCTTCACTGGATGCGGGTTGCGATATGATCCTGGTCTGCAATAATCGTAAAGGGGCCGTCAGCGTGTTAGATAATCTGTCACCGATCAAGGCAGAACGTGTTACACGTTTGTATCATAAAGGTTCATTTTcgc < 2:189112/269‑1 (MQ=255) gcgTCCGGCGAGCGGTTCTCCCTACTGGCTGAAAACCGTTTTGCGTCAGGAACTGGGTTTTGACGGCGTGATTTTCTCTGACGATTTATCGATGGAAGGTGCCGCGATTATGGGCAGTTATGCCGAACGCGGGCAGGCTTCACTGGATGCGGGTTGCGATATGATCCTGGTCTGCAATAATCGTAAAGGGGCCGTCAGCGTGTTAGATAATCTGTCACCGATCAAGGCAGAACGTGTTACACGTTTGTATCATAAAGGTTCATTTTcgc > 1:189112/1‑269 (MQ=255) cgTCCGGCGAGCGGTTCTCCCTACTGGCTGAAAACCGTTTTGCGTCAGGAACTGGGTTTTGACGGCGTGATTTTCTCTGACGATTTATCGATGGAAGGTGCCGCGATTATGGGCAGTTATGCCGAACGCGGGCAGGCTTCACTGGATGCGGGTTGCGATATGATCCTGGTCTGCAATAATCGTAAAGGGGCCGTCAGCGTGTTAGATAATCTGTCACCGATCAAGGCAGAACGTGTTACACGTTTGTATCATAAAGGTTCATTTTCGCGACAGGAACTGATGGACTCg > 1:86983/1‑288 (MQ=255) tCCGGCGAGCGGTTCTCCCTACTGGCTGAAAACCGTTTTGCGTCAGGAACTGGGTTTTGACGGCGTGATTTTCTCTGACGATTTATCGATGGAAGGTGCCGCGATTATGGGCAGTTATGCCGAACGCGGGCAGGCTTCACTGGATGCGGGTTGCGATATGATCCTGGTCTGCAATAATCGTAAAGGGGCCGTCAGCGTGTTAGATAATCTGTCACCGATCAAGGCAGAACGTGTTACACGTTTGTATCATAAAGGTTCATTTTCGCGACAGGAACTGATGGACTCGGc > 2:101837/1‑288 (MQ=255) cGAGCGGTTCTCCCTACTGGCTGAAAACCGTTTTGCGTCAGGAACTGGGTTTTGACGGCGTGATTTTCTCTGACGATTTATCGATGGAAGGTGCCGCGATTATGGGCAGTTATGCCGAACGCGGGCAGGCTTCACTGGATGCGGGTTGCGATATGATCCTGGTCTGCAATAATCGTAAAGGGGCCGTCAGCGTGTTAGATAATCTGTCACCGATCAAGGCAGAACGTGTTACACGTTTGTATCATAAAGGTTCATTTTCGCGACAGGAACTGATGGACTCGGCTCGCt < 2:169784/288‑1 (MQ=255) cGGTTCTCCCTACTGGCTGAAAACCGTTTTGCGTCAGGAACTGGGTTTTGACGGCGTGATTTTCTCTGACGATTTATCGATGGAAGGTGCCGCGATTATGGGCAGTTATGCCGAACGCGGACAGGCTTCACTGGATGCGGGTTGCGATATGATCCTGGTCTGCAATAATCGTAAAGGGGCCGTCAGCGTGTTAGATAATCTGTCACCGATCAAGGCAGAACGTGTTACACGTTTGTATCATAAAGGTTCATTTTCGCGACAGGAACTGATGGACTCGGCTCGCTGGaaa > 1:230066/1‑289 (MQ=255) aaCCGTTTTGCGTCAGGAACTGGGTTTTGACGGCGTGATTTTCTCTGACGATTTATCGATGGAAGGTGCCGCGATTATGGGCAGTTATGCCGAACGCGGGCAGGCTTCACTGGATGCGGGTTGCGATATGATCCTGGTCTGCAATAATCGTAAAGGGGCCGTCAGCGTGTTAGATAATCTGTCACCGATCAAGGCAGAACGTGTTACACGTTTGTATCATAAAGGTTCATTTTCGCGACAGGAACTGATGGACTCGGCTCGCTGGAAAGCGATCAGCACCCGTCTGAAt < 1:101837/289‑1 (MQ=255) tttGCGTCAGGAACTGGGTTTTGACGGCGTGATTTTCTCTGACGATTTATCGATGGAAGGTGCCGCGATTATGGGCAGTTATGCCGAACGCGGGCAGGCTTCACTGGATGCGGGTTGCGATATGATCCTGGTCTGCAATAATCGTAAAGGGGCCGTCAGCGTGTTAGATAATCTGTCACCGATCAAGGCAGAACGTGTTACACGTTTGTATCATAAAGGTTCATTTTCGCGACAGGAACTGATGGACTCGGCTCGCTGGAAAGCGATCAGCACCCGTCTGAATCAGtt > 1:180683/1‑288 (MQ=255) gggTTGTGACGGCGTGATTTTCTCTAACGATTAATCGATGGAAGTGACCGCGATTATGGGCAGTTATGCCGAACGCGGGCAGGTTTCAGTGGATGCGGGTTGCGATATGATCCTGGTCTGCAATAATCGTAAAGGGGCCGTCAGCGTGTTAGATAATCTGTCACCGATCAAGGCAGAACGTGTTACACGTTTGTATCATAAAGGTTCATTTTCGCGACAGGAACTGATGGACTCGGCTCGCTGGAAAGCGATCAGCACCCGTCTGAATCAGTTACATGAACGCTGGCAg < 1:155761/289‑1 (MQ=255) gCGTGATTTTCTCTGACGATTTATCGATGGAAGGTGCCGCGATTATGGGCAGTTATGCCGAACGCGGGCAGGCTTCACTGGATGCGGGTTGCGATATGATCCTGGTCTGCAATAATCGTAAAGGGGCCGTCAGCGTGTTAGATAATCTGTCACCGATCAAGGCAGAACGTGTTACACGTTTGTATCATAAAGGTTCATTTTCGCGACAGGAACTGATGGACTCGGCTCGCTGGAAAGCGATCAGCACCCGTCTGAATCAGTTACATGAACGCTGGCAGGAAGAGAAAGc > 2:208700/1‑289 (MQ=255) gCGTGATTTTCTCTGACGATTTATCGATGGAAGGTGCCGCGATTATGGGCAGTTATGCCGAACGCGGGCAGGCTTCACTGGATGCGGGTTGCGATATGATCCTGGTCTGCAATAATCGTAAAGGGGCCGTCAGCGTGTTAGATAATCTGTCACCGATCAAGGCAGAACGTGTTACACGTTTGTATCATAAAGGTTCATTTTCGCGACAGGAACTGATGGACTCGGCTCGCTGGAAAGCGATCAGCACCCGTCTGAATCAGTTACATGAACGCTGGCAGGAAGAGAAAGc > 1:174648/1‑289 (MQ=255) tctGACGATTTATCGATGGAAGGTGCCGCGATTATGGGCAGTTATGCCGAACGCGGGCAGGCTTCACTGGATGCGGGTTGCGATATGATCCTGGTCTGCAATAATCGTAAAGGGGCCGTCAGCGTGTTAGATAATCTGTCACCGATCAAGGCAGAACGTGTTACACGTTTGTATCATAAAGGTTCATTTTCGCGACAGGAACTGATGGACTCGGCTCGCTGGAAAGCGATCAGCACCCGTCTGAATCAGTTACATGAACGCTGGCAGGAAGAGAAAGCAGGTCACTaa > 2:344639/1‑288 (MQ=255) ctGACGATTTTTCGATGGAAGGTGCCGCGATTAGGGGCAGTTAGGCCGAACGAGGGCAGGGTTCACTGGATGCGGGTTGCGATTTGATCCGGGTCTGCAATAATCGTAAAGGGGCCGTCAGCGTGTTAGATAATCTGTCACCGATCAAGGCAGAACGTGTTACACGTTTGTATCATAAAGGTTCATTTTCGCGACAGGAACTGATGGACTCGGCTCGCTGGAAAGCGATCAGCACCCGTCTGAATCAGTTACATGAACGCTGGCAGGAAGAGAAAGCAGGTCACTAAcc < 2:31697/289‑1 (MQ=255) tATCGATGGAAGGTGCCGCGATTATGGGCAGTTATGCCGAACGCGGGCAGGCTTCACTGGATGCGGGTTGCGATATGATCCTGGTCTGCAATAATCGTAAAGGGGCCGTCAGCGTGTTAGATAATCTGTCACCGATCAAGGCAGAACGTGTTACACGt > 2:259383/1‑158 (MQ=255) tATCGATGGAAGGTGCCGCGATTATGGGCAGTTATGCCGAACGCGGGCAGGCTTCACTGGATGCGGGTTGCGATATGATCCTGGTCTGCAATAATCGTAAAGGGGCCGTCAGCGTGTTAGATAATCTGTCACCGATCAAGGCAGAACGTGTTACACGt < 1:259383/158‑1 (MQ=255) ggTGCCGCGATTATGGGCAGTTATGCCGAACGCGGGCAGGCTTCACTGGATGCGGGTTGCGATATGATCCTGGTCTGCAATAATCGTAAAGGGGCCGTCAGCGTGTTAGATAATCTGTCACCAATCAAGGCAGAACGTGTTACACGTTTGTATCATAAAGGTTCATTTTCGCGACAGGAACTGATGGACTCGGCTCGCTGGAAAGCGATCAGCACCCGTCTGAATCAGTTACATGAACGCTGGCAGGACGAGAAAGCAGGTCACTAACCCTGGCTTATGTGAGGAAGCg > 1:235667/1‑289 (MQ=255) ccGCGATTATGGGCAGTTATGCCGAACGCGGGCAGGCTTCACTGGATGCGGGTTGCGATATGATCCTGGTCTGCAATAATCGTAAAGGGGCCGTCAGCGTGTTAGATAATCTGTCACCGATCAAGGCAGAACGTGTTACACGTTTGTATCATAAAGGTTCATTTTCGCGACAGGAACTGATGGACTCGGCTCGCTGGAAAGCGATCAGCACCCGTCTGAATCAGTTACATGAACGCTGGCAGGAAGAGAAAGCAGGTCACTAACCCTGGCTTATGTGAGGAAGCgatga > 2:6060/1‑289 (MQ=255) gATTATGGGCAGTTATGCCGAACGCGGGCAGGCTTCACTGGATGCGGGTTGCGATATGATCCTGGTCTGCAATAATCGTAAAGGGGCCGTCAGCGTGTTAGATAATCTGTCACCGATCAAGGCAGAACGTGTTACACGTTTGTATCATAAAGGTTCATTTTCGCGACAGGAACTGATGGACTCGGCTCGCTGGAAAGCGATCAGCACCCGTCTGAATCAGTTACATGAACGCTGGCAGGAAGAGAAAGCAGGTCACTAACCCTGGCTTATGTGAGGAAGCGATGATTa < 2:134265/288‑1 (MQ=255) gATTATGGGCAGTTATGCCGAACGCGGACAGGCTTCACTGGATGCGGGTTGCGATATGATCCTGGTCTGCAATAATCGTAAAGGGGCCGTCAGCGTGTTAGATAATCTGTCACCGATCAAGGCAGAACGTGTTACACGTTTGTATCATAAAGGTTCATTTTCGCGACAGGAACTGATGGACTCGGCTCGCTGGAAAGCGATCAGCACCCGTCTGAATCAGTTACATGAACGCTGGCAGGAAGAGAAAGCAGGTCACTAACCCTGGCTTATGTGAGGAAGCGATGATTa < 2:230066/288‑1 (MQ=255) aGTTATGCCGAACGCGGGCAGGCTTCACTGGATGCGGGTTGCGATATGATCCTGGTCTGCAATAATCGTAAAGGGGCCGTCAGCGTGTTAGATAATCTGTCACCGATCAAGGCAGAACGTGTTACACGTTTGTATCATAAAGGTTCATTTTCGCGACAGGAACTGATGGACTCGGCTCGCTGGAAAGCGATCAGCACCCGTCTGAATCAGTTACATGAACGCTGGCAGGAAGAGAAAGCAGGTCACTAACCCTGGCTTATGTGAGGAAGCGATGATTATCTATTTaca < 1:208700/288‑1 (MQ=255) ttATGCCGAACGCGGGCAGGCTTCACTGGATGCGGGTTGCGATATGATCCTGGTCTGCAATAATCGTAAAGGGGCCGTCAGCGTGTTAGATAATCTGTCACCGATCAAGGCAGAACGTGTTACACGTTTGTATCATAAAGGTTCATTTTCGCGACAGGAACTGATGGAGTCGGCTCGCTGGAAAGCGATCAGCACCCGTCTGAATCAGTTACCTGAACGCTGGCAGGGAGAGAAAGCAGGTCCCTAACCCTGGCTTAGGGGAGGAAGTGATGGTTAGTCATTTACACgg > 2:140556/1‑289 (MQ=255) ttATGCCGAACGCGGGCAGGCTTCACTGGATGCGGGTTGCGATATGATCCTGGTCTGCAATAATCGTAAAGGGGCCGTCAGCGTGTTAGATAATCTGTCACCGATCAAGGCAGAACGTGTTACACGTTTGTATCATAAAGGTTCATTTTCGCGACAGGAACTGATGGACTCGGCTCGCTGGAAAGCGATCAGCACCCGTCTGCATCAGTTACATGAACGCTGGCATGAAGAGAAAGCAGGTCACTAACCCTGGCTTATGTGAGGAAGCGATGATTATCTATTTACACgg > 2:480529/1‑289 (MQ=255) aggCCGAACTCGGGCAGGCTTCACTGGGTGCGGGTTGCGTTATGATCCTGGTCTGCAATAATCGTAAAGGGGCCGTCAGCGTGTTAGATAATCTGTCACCAATCAAGGCAGAACGTGTTACACGTTTGTATCATAAAGGTTCATTTTCGCGACAGGAACTGATGGACTCGGCTCGCTGGAAAGCGATCAGCACCCGTCTGAATCAGTTACATGAACGCTGGCAGGAAGAGAAAGCAGGTCACTAACCCTGGCTTATGTGAGGAAGCGATGATTATCTATTTACACGGtt < 2:235667/287‑1 (MQ=255) aTGGCGAACGCAGGCAGGCTTCACTGGGTGCGGGTTGCGATATGATCCTGGTCTGCAATAATCGTAAAGGGGCCGTCAGCGTGTTAGATAATCTGTCACCGATCAAGGCAGAACGTGTTACACGTTTGTATCATAAAGGTTCATGTTCGCGACAGGAACTGATGGACTCGGCTCGCTGGAAAGCGATCAGCACCCGTCTGAATCAGTTACATGAACGCTGGCAGGAAGAGAAAGCAGGTCACTAACCCTGGCTTATTTGAGGAAGCGATGATTATCTATTTACACGGtt < 1:264356/289‑1 (MQ=255) aTGCCGAACGCGGGCAGGCTTCACTGGATGCGGGTTGCGATATGATCCTGGTCTGCAATAATCGTAAAGGGGCCGTCAGCGTGTTAGATAATCTGTCACCGATCAAGGCAGAACGTGTTACACGTTTGTATCATAAAGGTTCATTTTCGCGACAGGAACTGATGGACTCGGCTCGCTGGAAAGCGATCAGCACCCGTCTGAATCAGTTACATGAACGCTGGCAGGAAGAGAAAGCAGGTCACTAACCCTGGCTTATGTGAGGAAGCGATGATTATCTATTTACACGGtt < 2:174648/289‑1 (MQ=255) cGAACGCGGGCAGGCTTCACTGGATGCGGGTTGCGATATGATCCTGGTCTGCAATAATCGTAAAGGGGCCGTCAGCGTGTTAGATAATCTGTCACCGATCAAGGCAGAACGTGTTACACGTTTGTATCATAAAGGTTCATTTTCGCGACAGGAACTGATGGACTCGGCTCGCTGGAAAGCGATCAGCACCCGTCTGAATCAGTTACATGAACGCTGGCAGGAAGAGAAAGCAGGTCACTAACCCTGGCTTATGTGAGGAAGCGATGATTATCTATTTACACGGTTTTGa < 1:10603/289‑1 (MQ=255) cGAACGCGGGCAGGCTTCACTGGATGCGGGTTGCGATATGATCCTGGTCTGCAATAATCGTAAAGGGGCCGTCAGCGTGTTAGATAATCTGTCACCGATCAAGGCAGAACGTGTTACACGTTTGTATCATAAAGGTTCATTTTCGCGACAGGAACTGATGGACTCGGCTCGCTGGAAAGCGATCAGCACCCGTCTGAATCAGTTACATGAACGCTGGCAGGAAGAGAAAGCAGGTCACTAACCCTGGCTTATGTGAGGAAGCGATGATTATCTATTTACACGGTTTTGa < 1:322688/289‑1 (MQ=255) tGGATGCGGGTTGCGATATGATCCTGGTCTGCAATAATCGTAAAGGGGCCGTCAGCATGTTAGATTATCTGTCACCGATCAAGGCAGGACGTGTTACACGTTTGTATCATAAAGGTTCATTTTCGCGACAGGAACTGATGGACTCGGCTCGCTGGAAAGCGATCAGCACCCGTCTGAATCAGTTACATGAACGCTGGCAGGAAGAGAAAGCAGGTCACTAACCCTGGCTTATGTGAGGAAGCGATGATTATCTATTTACACGGTTTTGACTCTAACAGTCCGGGTAAcc < 1:365909/289‑1 (MQ=255) aTGCGGGTTGCGATATGATCCTGGTCTGCAATAATCGTAAAGGGGCCGTCAGCGTGTTAGATAATCTGTCACCGATCAAGGCAGAACGTGTTACACGTTTGTATCATAAAGGTTCATTTTCGCGACAGGAACTGATGGACTCGGCTCGCTGGAAAGCGATCAGCACCCGTCTGAATCAGTTACATGAACGCTGGCAGGAAGAGAAAGCAGGTCACTAACCCTGGCTTATGTGAGGAAGCGATGATTATCTATTTaca < 1:125125/257‑1 (MQ=255) aTGCGGGTTGCGATATGATCCTGGTCTGCAATAATCGTAAAGGGGCCGTCAGCGTGTTAGATAATCTGTCACCGATCAAGGCAGAACGTGTTACACGTTTGTATCATAAAGGTTCATTTTCGCGACAGGAACTGATGGACTCGGCTCGCTGGAAAGCGATCAGCACCCGTCTGAATCAGTTACATGAACGCTGGCAGGAAGAGAAAGCAGGTCACTAACCCTGGCTTATGTGAGGAAGCGATGATTATCTATTTaca > 2:125125/1‑257 (MQ=255) aTGCGGGTTGCGATATGATCCTGGTCTGCAATAATCGTAAAGGGGCCGTCAGCGTGTTAGATAATCTGTCACCGATCAAGGCAGAACGTGTTACACGTTTGTATCATAAAGGTTCATTTTCGCGACAGGAACTGATGGACTCGGCTCGCTGGAAAGCGATCAGCACCCGTCTGAATCAGTTACATGAACGCTGGCAGGAAGAGAAAGCAGGTCACTAACCCTGGCTTATGTGAGGAAGCGATGATTATCTATTTACACGGTTTTGACTCTAACAGTCCGGGTAACCACg > 2:310663/1‑289 (MQ=255) ccTGGTCTGCAATAATCGTAAAGGGGCCGTCAGCGTGTTAGATAATCTGTCACCGATCAAGGCAGAACGTGTTACACGTTTGTATCATAAAGGTTCATTTTCGCGACAGGAACTGATGGACTCGGCTCGCTGGAAAGCGATCAGCACCCGTCTGAATCAGTTACATGAACGCTGGCAGGAAGAGAAAGCAGGTCACTAACCCTGGCTTATGTGAGGAAGCGATGATTATCTATTTACACGGTTTTGACTCTAACAGTCCGGGTAACCACGAGAAAGTCTTACAATTGCa < 1:344639/289‑1 (MQ=255) gTCTGCAATAATCGTAAAGGGGCCGTCAGCGTGTTAGATAATCTGTCACCGATCAAGGCAGAACGTGTTACACGTTTGTATCATAAAGGTTCATTTTCGCGACAGGAACTGATGGACTCGGCTCGCTGGa < 2:124053/130‑1 (MQ=255) gTCTGCAATAATCGTAAAGGGGCCGTCAGCGTGTTAGATAATCTGTCACCGATCAAGGCAGAACGTGTTACACGTTTGTATCATAAAGGTTCATTTTCGCGACAGGAACTGATGGACTCGGCTCGCTGGa > 1:124053/1‑130 (MQ=255) gTCTGCAATAATCGTAAAGGGGCCGTCAGCGTGTTAGATAATCTGTCACCGATCAAGGCAGAACGTGTTACACGTTTGTATCATAAAGGTTCATTTTCGCGACAGGAACTGATGGACTCGGCTCGCTGGAAAGCGATCAGCACCCGTCTGAATCAGTTACATGAACGCTGGCAGGAAGAGAAAGCAGGTCACTAACCCTGGCTTATGTGAGGAAGCGATGATTATCTATTTACACGGTTTTGACTCTAACAGTCCGGGTAACCACgaga < 2:336005/269‑1 (MQ=255) gTCTGCAATAATCGTAAAGGGGCCGTCAGCGTGTTAGATAATCTGTCACCGATCAAGGCAGAACGTGTTACACGTTTGTATCATAAAGGTTCATTTTCGCGACAGGAACTGATGGACTCGGCTCGCTGGAAAGCGATCAGCACCCGTCTGAATCAGTTACATGAACGCTGGCAGGAAGAGAAAGCAGGTCACTAACCCTGGCTTATGTGAGGAAGCGATGATTATCTATTTACACGGTTTTGACTCTAACAGTCCGGGTAACCACgaga > 1:336005/1‑269 (MQ=255) gTCTGCAATAATCGTAAAGGGGCCGTCAGCGTGTTAGATAATCTGTCACCGATCAAGGCAGAACGTGTTACACGTTTGTATCATAAAGGTTCATTTTCGCGACAGGAACTGATGGACTCGGCTCGCTGGAAAGCGATCAGCACCCGTCTGAATCAGTTACATGAACGCTGGCAGGAAGAGAAAGCAGGTCACTAACCCTGGCTTATGTGAGGAAGCGATGATTATCTATTTACACGGTTTTGACTCTAACAGTCCGGGTAACCACGAGAAAGTCTTACAATTGCAGttt > 1:325000/1‑289 (MQ=255) cTGCAATAATCGTAAAGGGGCCGTCAGCGTGTTAGATAATCTGTCACCGATCAAGGCAGAACGTGTTACACGTTTGTATCATAAAGGTTCATTTTCGCGACAGGAACTGATGGACTCGGCTCGCTGGAAAGCGATCAGCACCCGTCTGAATc < 1:224426/152‑1 (MQ=255) cTGCAATAATCGTAAAGGGGCCGTCAGCGTGTTAGATAATCTGTCACCGATCAAGGCAGAACGTGTTACACGTTTGTATCATAAAGGTTCATTTTCGCGACAGGAACTGATGGACTCGGCTCGCTGGAAAGCGATCAGCACCCGTCTGAATc > 2:224426/1‑152 (MQ=255) tGCAATAATCGTAAAGGGGCCGTCAGCGTGTTAGATAATCTGTCACCGATCAAGGCAGAACGTGTTACACGTTTGTATCATAAAGGTTCATTTTCGCGACAGGAACTGATGGACTCGGCTCGCTGGAAAGCGATCAGCACCCGTCTGAATCAGTTACATGAACGCTGGCAGGAAGAGAAAGCAGGTCACTAACCCTGGCTTATGTGAGGAAGCGATGATTATCTATTTACACGGTTTTGACTCTAACAGTCCGGGTAACCACGAGAAAGTCTTACAATTGCAGTTTAtt < 1:310663/289‑1 (MQ=255) aaaGGGGCCGTCAGCGTGTTAGATAATCTGTCACCGATCAAGGCAGAACGTGTTACACGTTTGTATCATAAAGGTTCATTTTCGCGACAGGAACTGATGGACTCGGCTCGCTGGAAAGCGATCAGCACCCGTCTGAATCAGTTACATGAACGCTGGCAGGAAGAGAAAGCAGGTCACTAACCCTGGCTTATGTGAGGAAGCGATGATTATCTATTTACACGGTTTTGACTCTAACAGTCCGGGTAACCACGAGAAAGTCTTACAATTGCAGTTTATTGACCCGGATGt > 2:58672/1‑288 (MQ=255) ccGTCAGCGTGTTAGATAATCTGTCACCGATCAAGGCAGAACGTGTTACACGTTTGTATCATAAAGGTTCATTTTCGCGACAGGAACTGATGGACTCGGCTCGCTGGAAAGCGATCAGCACCCGTCTGAATCAGTTACATGAACGCTGGCAGGAAGAGAAAGCAGGTCACTAACCCTGGCTTATGTGAGGAAGCGATGATTATCTATTTACACGGTTTTGACTCTAACAGTCCGGGTAACCACGAGAAAGTCTTACAATTGCAGTTTATTGACCCGGATGTACGCTTg < 2:260649/288‑1 (MQ=255) ccGTCAGCGTGTTAGATAATCTGGCACCGATAAAGGCAGAACGTGTTACACGTTTGTATCATAAAGGTTCATTTTCGCGACAGGAACTGATGGACTCGGCTCGCTGGAAAGCGATCAGCACCCGTCTGAATCAGTTACATGAACGCTGGCAGGAAGAGAAAGCAGGTCACTAACCCTGGCTTATGTGAGGAAGCGATGATTATCTATTTACACGGTTTTGACTCTAACAGTCCGGGTAACCACGAGAAAGTCTTACAATTGCAGTTTATTGACCCGGATGTACGCTTg < 1:398106/288‑1 (MQ=255) aCCGATCAAGGCAGAACGTGTTACACGTTTGTATCATAAAGGTTCATTTTCGCGACAGGAACTGATGGACTCGGCTCGCTGGAAAGCGATCAGCACCCGTCTGAATCAGTTACATGAACGCTGGCAGGAAGAGAAAGCAGGTCACTAACCCTGGCTTATGTGAGGAAGCGATGATTATCTATTTACACGGTTTTGACTCTAACAGTCCGGGTAACCACGAGAAAGTCTTACAATTGCAGTTTATTGACCCGGATGTACGCTTGATAAGCTACAGTACGCGGCATCCGaa < 2:180683/289‑1 (MQ=255) | CCTTAATCCGCGAAAATAAACTCGACGCCATTATGCCTGCGCATGTGATCTACAGTGATGTTGATCCGCGTCCGGCGAGCGGTTCTCCCTACTGGCTGAAAACCGTTTTGCGTCAGGAACTGGGTTTTGACGGCGTGATTTTCTCTGACGATTTATCGATGGAAGGTGCCGCGATTATGGGCAGTTATGCCGAACGCGGGCAGGCTTCACTGGATGCGGGTTGCGATATGATCCTGGTCTGCAATAATCGTAAAGGGGCCGTCAGCGTGTTAGATAATCTGTCACCGATCAAGGCAGAACGTGTTACACGTTTGTATCATAAAGGTTCATTTTCGCGACAGGAACTGATGGACTCGGCTCGCTGGAAAGCGATCAGCACCCGTCTGAATCAGTTACATGAACGCTGGCAGGAAGAGAAAGCAGGTCACTAACCCTGGCTTATGTGAGGAAGCGATGATTATCTATTTACACGGTTTTGACTCTAACAGTCCGGGTAACCACGAGAAAGTCTTACAATTGCAGTTTATTGACCCGGATGTACGCTTGATAAGCTACAGTACGCGGCATCCGAA > NC_000913/1164690‑1165261 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |