Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,560,882 A→T 6.4% intergenic (‑202/‑48) yjiH ← / → kptA nucleoside recognition pore and gate family putative inner membrane transporter/RNA 2'‑phosphotransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,560,8820AT 6.4% 220.3 / 3.6 62intergenic (‑202/‑48)yjiH/kptAnucleoside recognition pore and gate family putative inner membrane transporter/RNA 2'‑phosphotransferase
Reads supporting (aligned to +/- strand):  ref base A (26/32);  new base T (2/2);  total (28/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

TGGTGAGAAAAAAGGCCAGATACCCTTTAATGCCCACTTTTTCTGTCGCCAGCTCCCCGGCTACAGCATCCCCTTGTTGTGTCATAACAATCCCCATTGTTTTTTTGTGTTTGTGTGTTGCTTGAGCCAGGAAAATTCCTGGAACTGAAAAACCTTATCAGCCATGTGGGTATGGGGAAAATGCAAGTATGTTTCTGGCTATGCGTTTTTTGCATAGCGGAAAAATTACGCTGTGTGGAAAAGGCTCTGCGCAGGATTGAGTTGCAGAAAATTCATAAACCAAGTGACAATGACATCAGTAAATAAAAACAGAAAATCCAGGGACGAAAACGAGATGGCAAAGTACAACGAAAAAGAGCTGGCGGATACGAGCAAGTTTTTAAGTTTTGTCCTCCGACACAAACCGGAGGCGATTGGTATCGTACTGGACCGTGAAGGATGGGCGGATATCGATAAGCTCATACTTTGTGCCCAAAAAGCGGGCAAGCGGTTAACCCGTGCATTGCTGGATACTGTGGTTGCCACCAGCGATAAAAAGCGTTTTAGTTATTCCAGTGATGGTAGGTGCATCCGCG  >  NC_000913/4560596‑4561170
                                                                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                                                                
tGGTGAGAAAAAAGGCCAGATACCCTTTAATGCCCACTTTTTCTGTCGCCAGCTGCCCGGCTACAGCATCCCCTTGTTGTGTCATAACAATCCCCATTGTTTTTTTGTGTTTGTGTGTTGCTTGAGCCAGGAAAATTCCTGGAACTGAAAAACCTTATCAGCCATGTGGGTATGGGGAAAATGCAAGTATGTTTCTGGCTATGCGTTTTTTGCATAGCGGAAAAATTACGCTGTGTGGAAAAGGCTCTGCGCAGGATTGAGTTGCAGAAAATTCATAAACCAAGTGACa                                                                                                                                                                                                                                                                                                <  2:128993/289‑1 (MQ=255)
                 aGATACCCTTTAATGCCCACTTTTTCTGTCGCCAGCTCCCCGGCTACAGCATCCCCTTGTTGTGTCATAACAATCCCCATTGTTTTTTTGTGTTTGTGTGTTGCTTGAGCCAGGAAAATTCCTGGAACTGAAAAACCTTATCAGCCATGTGGGTATGGGGAAAATGCAAGTATGTTTCTGGCTATGCGTTTTTTGCATAGCGGAAAAATTACGCTGTGTGGAAAAGGCTCTGCGCAGGATTGAGTTGCAGAAAATTCATAAACCAAGTGACAATGACATCAGtaaataa                                                                                                                                                                                                                                                                               >  2:82733/1‑289 (MQ=255)
                 aGATACCCTTTAATGCCCACTTTTTCTGTCGCCAGCTCCCCGGCTACAGCATCCCCTTGTTGTGTCATAACAATCCCCATTGTTTTTTTGTGTTTGTGTGTTGCTTGAGCCAGGAAAATTCCTGGAACTGAAAAACCTTATAAGCCATGTGGGTATGGGGAAAATGCAAGTATGTTTCTGGCTATGCGTTTTTTGCATAGCGGAAAAATTACGCTGTGTGGAAAAGGCTCTGCGCAGGCTTGAGTTGAAGAAAATTCATAAACCAAGTGACAATGACTTAAGtaaataa                                                                                                                                                                                                                                                                               >  1:481132/1‑289 (MQ=255)
                        ctgtAATGCCCACTTTTTCTGTCGCCAGCTCCCCGGCTACAGCATCCCCTTGTTGTGTCATAACAATCCCCATTGTTTTTTTGTGTTTGTGTGTTGCTTGAGCCAGGAAAATTCCTGGAACTGAAAAACCTTATCAGCCATGTGGGTATGGGGAAAATGCAAGTATGTTTCTGGCTATGCGTTTTTTGCATAGCGGAAAAATTACGCTGTGTGGAAAAGGCTCTGCGCAGGATTGAGTTGCAGAAAATTCATAAACCAAGTGACAATGACATCAGTAAATAAAAACAGa                                                                                                                                                                                                                                                                        <  2:511160/286‑1 (MQ=255)
                        ccgtAAAGCCCACTTTTTCCGTAGCCAGCTCCCCGGCTGCATCATACCCTTGTTGTGGCAGAACAAGCCCCATTGGTGTTTTGGGTTGGTGGGTTGCGTGAGCCAGGAAAATTCCTGGAACTGAAAAACCTTATCAGCCATGTGGGTATGGGGAAAATGCAAGTATGTTTCTGGCTATGCGTTTTTTGCATAGCGGAAAAATTACGCTGTGTGGAAAAGGCTCTGCGCAGGATTGAGTTGCAGAAAAGTCATAAACCAAGTGACAATGACATCAGTAAATAAAAACAGa                                                                                                                                                                                                                                                                        <  2:266822/286‑1 (MQ=255)
                        cTTTAATGCCCACTTTTTCTGTCGCCAGCTCCCCGGCTACAGCATCCCCTTGTTGTGTCATAACAATCCCCATTGTTTTTTTGTGTTTGTGTGTTGCTTGAGCCAGGAAAATTCCTGGAACTGAAAAACCTTATCAGCCATGTGGGTATGGGGAAAATGCAAGTATGTTTCTGGCTATGCGTTTTTTGCATAGCGGAAAAATTACGCTGTGTGGAAAAGGCTCTGCGCAGGATTGAGTTGCAGAAAATTCATAAACCAAGTGACAATGACATCAGTAAATAAAAACAGa                                                                                                                                                                                                                                                                        <  2:393025/289‑1 (MQ=255)
                                 ccACTTTTTCTGTCGCCAGCTCCCCGGCTACAGCATCCCCTTGTTGTGTCATAACAATCCCCATTGTTTTTTTGTGTTTGTGTGTTGCTTGAGCCAGGAAAATTCCTGGAACTGAAAAACCTTATCAGCCATGTGGGTATGGGGAAAATGCAAGTATGTTTCTGGCTATGCGTTTTTTGCATAGCGGAAAAATTACGCTGTGTGGAAAAGGCTCTGCGCAGGATTGAGTTGCAGAAAATTCATAAACCAAGTGACAATGACATCAGTAAATAAAAACAGAAAATCCAgg                                                                                                                                                                                                                                                               >  1:321760/1‑289 (MQ=255)
                                              cGCCAGCTCCCCGGCTACAGCATCCCCTTGTTGTGTCATAACAATCCCCATTGTTTTTTTGTGTTTGTGTGTTGCTTGAGCCAGGAAAATTCCTGGAACTGAAAAACCTTATCAGCCATGTGGGTATGGGGAAAATGCAAGTATGTTTCTGGCTATGCGTTTTTTGCATAGCGGAAAAATTACGCTGTGTGGAAAAGGCTCTGCGCAGGATTGAGTTGCAGAAAATTCATAAACCAAGTGACAATGACATCAGTAAATAAAAACAGAAAATCCAGGGACGAAAACgaga                                                                                                                                                                                                                                                  <  1:82733/289‑1 (MQ=255)
                                                     tCCCCGGCTACAGCATCCCCTTGTTGTGTCATAACAATCCCCATTGTTTTTTTGTGTTTGTGTGTTGCTTGAGCCAGGAAAATTCCTGGAACTGAAAAACCTTATCAGCCATGTGGGTATGGGGAAAATGCAAGTATGTTTCTGGCTATGCGTTTTTTGCATAGCGGAAAAATTACGCTGTGTGGAAAAGGCTCTGCGCAGGATTGAGTTGCAGAAAATTCATAAACCAAGTGACAATGACATCAGTAAATAAAAACAGAAAATCCAGGGACGAAAACGAGATGGCa                                                                                                                                                                                                                                             <  1:26931/287‑1 (MQ=255)
                                                            cTACAGCATCCCCTTGTTGTGTCATAACAATCCCCATTGTTTTTTTGTGTTTGTGTGTTGCTTGAGCCAGGAAAATTCCTGGAACTGAAAAACCTTATCAGCCATGTGGGTATGGGGAAAATGCAAGTATGTTTCTGGCTATGCGTTTTTTGCATAGCGGAAAAATTACGCTGTGTGGAAAAGGCTCTGCGCAGGATTGAGTTGCAGAAAATTCATAAACCAAGTGACAATGACATCAGTAAATAAAAACAGAAAATCCAGGGACGAAAACGAGATGGCAAAGTACaa                                                                                                                                                                                                                                     <  2:120170/288‑1 (MQ=255)
                                                                      cccTTGTTGTGTCATAACAATCCCCATTGTTTTTTTGTGTTTGTGTGTTGCTTGAGCCAGGAAAATTCCTGGAACTGAAAAACCTTATCAGCCATGTGGGTATGGGGAAAATGCAAGTATGTTTCTGGCTATGCGTTTTTTGCATAGCGGAAAAATTACGCTGTGTGGAAAAGGCTCTGCGCAGGATTGAGTTGCAGAAAATTCATAAACCAAGTGACAATGACATCAGTAAATAAAAACAGAAAATCCAGGGACGAAAACGAGATGGCAAAGTACAACGAAAAaga                                                                                                                                                                                                                            >  1:101614/1‑287 (MQ=255)
                                                                             tgtaTCATAACAATCCCAGTTTGTTTTTTGTGTTTGTGTGTTGCTTGAGCCAGGAAAATTCCTGGAACTGAAAAACCTGATCAGCAATGTGGATATGGGGAGAATGCAAGTATTTTTCTGGCTATGCGTTGTTTGCATAGCGGAAAAAGTACGCTGTGTGGAAAAGGCTCTGCGCAGGATTGAGTTGCAGAAAATTCATAAACCAAGTGACAATGACATCAGTAAATAAAAACAGAAAATCCAGGGACGAAAACGAGATGGCAAAGTACAACGAAAAAGAGCTGGCGGa                                                                                                                                                                                                                   <  2:72027/289‑1 (MQ=255)
                                                                              gtgtCATAACAATCCCCATTGTTTTTTTGTGTTTGTGTGTTGCTTGAGCCAGGAAAATTCCTGGAACTGAAAAACCTTATCAGCCATGTGGGTATGGGGAAAATGCAAGTATGTTTCTGGCTATGCGTTTTTTGCATAGCGGAAAAATTACGCTGTGTGGAAAAGGCTCTGCGCAGGATTGAGTTGCAGAAAATTCATAAACCAAGTGTCAATGACATCAGTAAATAAAAACAGAAAATCCAGGGACGAAAACGAGATGGCAAAGTACAACGAAAAAGAGCTGGCGGAt                                                                                                                                                                                                                  >  1:281174/1‑289 (MQ=255)
                                                                              gtgtCATAACAATCCCCATTGTTTTTTTGTGTTTGTGTGTTGCTTGAGCCAGGAAAATTCCTGGAACTGAAAAACCTTATCAGCCATGTGGGTATGGGGAAAATGCAAGTATGTTTCTGGCTATGCGTTTTTTGCATAGCGGAAAAATTACGCTGTGTGGAAAAGGCTCTGCGCAGGATTGAGTTGCAGAAAATTCATAAACCAAGTGTCAATGACATCAGTAAATAAAAACAGAAAATCCAGGGACGAAAACGACATGGCAAAGTACAACGAAAAAGAGCTGGCGGAt                                                                                                                                                                                                                  >  1:281180/1‑289 (MQ=255)
                                                                                   aTAACAATCCCCATTGTTTTTTTGTGTTTGTGTGTTGCTTGAGCCAGGAAAATTCCTGGAACTGAAAAACCTTATCAGCCATGTGGGTATGGGGAAAATGCAAGTATGTTTCTGGCTATGCGTTTTTTGCATAGCGGAAAAATTACGCTGTGTGGAAAAGGCTCTGCGCAGGATTGAGTTGCAGAAAATTCATAAACCAAGTGACAATGACATCAGTAAATAAAAACAGAAAATCCAGGGACGAAAACGAGATGGCAAAGTACAACGAAAAAGAGCTGGCGGATACGa                                                                                                                                                                                                              >  1:152318/1‑288 (MQ=255)
                                                                                         aTCCCCATTGTTTTTTTGTGTTTGTGTGTTGCTTGAGCCAGGAAAATTCCTGGAACTGAAAAACCTTATCAGCCATGTGGGTATGGGGAAAATGCAAGTATGTTTCTGGCTATGCGTTTTTTGCATAGCGGAAAAATTACGCTGTGTGGAAAAGGCTCTGCGCAGGATTGAGTTGCAGAAAATTCATAAACCAAGTGACAATGACATCAGTAAATAAAAACAGAAAATCCAGGGACGAAAACGAGATGGCAAAGTACAACGAAAAAGAGCTGGCGGATACGAGCAAGtt                                                                                                                                                                                                       >  2:13278/1‑289 (MQ=255)
                                                                                           ccccATTGTTTTTTTGTGTTTGTGTGTTGCTTGAGCCAGGAAAATTCCTGGAACTGAAAAACCTTATCAGCCATGTGGGTATGGGGAAAATGCAAGTATGTTTCTGGCTATGCGTTTTTTGCATAGCGGAAAAATTACGCTGTGTGGAAAAGGCTCTGCGCAGGATTGAGTTGCAGAAAATTCATAAACCAAGTGACAATGACATCAGTAAATAAAAACAGAAAATCCAGGGACGAAAACGAGATGGCAAAGTACAACGAAAAAGAGCTGGCGGATACGAGCAAGtttt                                                                                                                                                                                                     >  1:159353/1‑289 (MQ=255)
                                                                                                             tttgtgtGTTGCTTGAGCCAGGAAAATTCCTGGAACTGAAAAACCTTATCAGCCATGTGGGTATGGGGAAAATGCAAGTATGTTTCTGGCTATGCGTTTTTTGCATAGCGGAAAAATTACGCTGTGTGGAAAAGGCTCTGCGCAGGATTGAGTTGCAGAAAATTCATAAACCAAGTGACAATGACATCAGTAAATAAAAACAGAAAATCCAGGGACGAAAACGAGATGGCAAAGTACAACGAAAAAGAGCTGGCGGATACGAGCAAGTTTTTAAGTTTTGTCCTCCGac                                                                                                                                                                                   >  1:87980/1‑289 (MQ=255)
                                                                                                                       gCTTGAGCCAGGAAAATTCCTGGAACTGAAAAACCTTATCAGCCATGTGGGTATGGGGAAAATGCAAGTATGTTTCTGGCTATGCGTTTTTTGCATAGCGGAAAAATTACGCTGTGTGGAAAAGGCTCTGCGCAGGATTGAGTTGCAGAAAATTCATAAACCAAGTGACAATGACATCAGTAAATAAAAACAGAAAATCCAGGGACGAAAACGAGATGGCAAAGTACAACGAAAAAGAGCTGGCGGATACGAGCAAGTTTTTAAGTTTTGTCCTCCGACACAAACCGGa                                                                                                                                                                         >  1:372015/1‑289 (MQ=255)
                                                                                                                        cTTGAGCCAGGAAAATTCCTGGAACTGAAAAACCTTATCAGCCATGTGGGTATGGGGAAAATGCAAGTATGTTTCTGGCTATGCGTTTTTTGCATAGCGGAAAAATTACGCTGTGTGGAAAAGGCTCTGCGCAGGATTGAGTTGCAGAAAATTCATAAACCAAGTGACAATGACATCAGTAAATAAAAACAGAAAATCCAGGGACGAAAACGAGATGGCAAAGTa                                                                                                                                                                                                                                        <  1:370200/225‑1 (MQ=255)
                                                                                                                        cTTGAGCCAGGAAAATTCCTGGAACTGAAAAACCTTATCAGCCATGTGGGTATGGGGAAAATGCAAGTATGTTTCTGGCTATGCGTTTTTTGCATAGCGGAAAAATTACGCTGTGTGGAAAAGGCTCTGCGCAGGATTGAGTTGCAGAAAATTCATAAACCAAGTGACAATGACATCAGTAAATAAAAACAGAAAATCCAGGGACGAAAACGAGATGGCAAAGTa                                                                                                                                                                                                                                        >  2:370200/1‑225 (MQ=255)
                                                                                                                              ccAGGAAAATTCCTGGAACTGAAAAACCTTATCAGCCATGTGGGTATGGGGAAAATGCAAGTATGTTTCTGGCTATGCGTTTTTTGCATAGCGGAAAAATTACGCTGTGTGGAAAAGGCTCTGCGCAGGATTGAGTTGCAGAAAATTCATAAACCAAGTGACAATGACATCAGTAAATAAAAACAGAAAATCCAGGGACGAAAACGAGATGGCAAAGTACAACGAAAAAGAGCTGGCGGATACGAGCAAGTTTTTAAGTTTTGTCCTCCGACACAAACCGGAGGCGAt                                                                                                                                                                   <  2:101614/288‑1 (MQ=255)
                                                                                                                               cAGGAAAATTCCTGGAACTGAAAAACCTTATCAGCCATGTGGGTATGGGGAAAATGCAAGTATGTTTCTGGCTATGCGTTTTTTGCATAGCGGAAAAATTACGCTGTGTGGAAAAGGCTCTGCGCAGGATTGAGTTGCAGAAAATTCATAAACCAAGTGACAATGACATCAGTAAATAAAAACAGAAAATCCAGGGACGAAAACGAGATGGCAAAGTACAACGAAAAAGAGCTGGCGGATACGAGCAAGTTTTTAAGTTTTGTCCTCCGACACAAACCGGAGGCGAt                                                                                                                                                                   <  2:429030/287‑1 (MQ=255)
                                                                                                                                   gaaaTTCCTGGAACTGAAAAACCTTATCAGCCATGTGGGTATGGGGAAAATGCAAGTATGTTTCTGGCTATGCGTTTTTTGCATAGCGGAAAAATTACGCTGTGTGGAAAAGGCTCTGCGCAGGATTGAGTTGCAGAAAATTCATAAACCAAGTGACAATGACATCAGTAAATAAAAACAGAAAATCCAGGGACGAAAACGAGATGGCAAAGTACAACGAAAAAGAGCTGGCGGATACGAGCAAGTTTTTAAGTTTTGTCCTCCGACACAAACCGGAGGCGATTGGTa                                                                                                                                                              <  2:156185/287‑1 (MQ=255)
                                                                                                                                   aaaaTTCCTGGAACTGAAAAACCTTATCAGCCATGTGGGTATGGGGAAAATGCAAGTATGTTTCTGGCTATGCGTTTTTTGCATAGCGGAAAAATTACGCTGTGTGGAAAAGGCTCTGCGCAGGATTGAGTTGCAGAAAATTCATAAACCAAGTGACAATGACATCAGTAAATAAAAACAGAAAATCCAGGGACGAAAACGAGATGGCAAAGTACAACGAAAAAGAGCTGGCGGATACGAGCAAGTTTTTAAGTTTTGTCCTCCGACACAAACCGGAGGCGATTGGTa                                                                                                                                                              <  2:372015/288‑1 (MQ=255)
                                                                                                                                   aaaaTTCCTGGAACTGAAAAACCTTATCAGCCATGTGGGTATGGGGAAAATGCAAGTATGTTTCTGGCTATGCGTTTTTTGCATAGCGGAAAAATTACGCTGTGTGGAAAAGGCTCTGCGCAGGATTGAGTTGCAGAAAATTCATAAACCAAGTGACAATGACATCAGTAAATAAAAACAGAAAATCCAGGGACGAAAACGAGATGGCAAAGTACAACGAAAAAGAGCTGGCGGATACGAGCAAGTTTTTAAGTTTTGTCCTCCGACACAAACCGGAGGCGATTGGTa                                                                                                                                                              <  2:159353/288‑1 (MQ=255)
                                                                                                                                           tGGAACTGAAAAACCTTATCAGCCAGGTGGGTATGGGGAAAATGCAAGTATGTTTCTGGCTATGCGTTTTTTGCATAGCGGAAAAATTACGCTGTGTGGAAAAGGCTCTGCGCAGGATTGAGTTGCAGAAAATTCATAAACCAAGTGACAATGACATCAGTAAATAAAAACAGAAAATCCAGGGACGAAAACGAGATGGCAAAGTACAACGAAAAAGAGCTGGCGGATACGAGCAAGTTTTTAAGTTTTGTCCTCCGACACAAACCGGAGGCGATTGGTATCGTACTgg                                                                                                                                                     <  2:216790/289‑1 (MQ=255)
                                                                                                                                            ggAACTGAAAAACCTTATCAGCCATGTGGGTATGGGGAAAATGCAAGTATGTTTCTGGCTATGCGTTTTTTGCATAGCGGAAAAATTACGCTGTGTGGAAAAGGCTCTGCGCAGGATTGAGTTGCAGAAAATTCATAAACCAAGTGACAATGACATCAGTAAATAAAAACAGAAAATCCAGGGACGAAAACGAGATGGCAAAGTACAACGAAAAAGAGCTGGCGGATACGAGCAAGTTTTTAAGTTTTGTCCTCCGACACAAACCGGAGGCGATTGGTATCGTACTGGa                                                                                                                                                    <  2:321760/289‑1 (MQ=255)
                                                                                                                                                      aaCCTTATCAGCCATGTGGGTATGGGGAAAATGCAAGTATGTTTCTGGCTATGCGTTTTTTGCATAGCGGAAAAATTACGCTGTGTGGAAAAGGCTCTGCGCAGGATTGAGTTGCAGAAAATTCATAAACCAAGTGTCAATGACATCAGTAAATAAAAACAGAAAATCCAGGGACGAAAACGAGATGGCAAAGTACAACGAAAAAGAGCTGGCGGATACGAGCAAGTTTTTAAGTTTTGTCCTCCGACACAAACCGGAGGCGATTGGTATCGTACTGGACCGTGAAGGa                                                                                                                                          <  2:281174/289‑1 (MQ=255)
                                                                                                                                                      aaCCATATCAGCAATGTGGGTATGGGTAAAATGCAAGTATGTGTCTGGCTATGCGTTTTTTGCATAGCGGAAAAATTACGCTGTGTGGAAAAGGCTCTGCGCAGGATTGAGTTGCAGAAAATTCATAAACCAAGTGTCAATGACATCAGTAAATAAAAACAGAAAATCCAGGGACGAAAACGAGATGGCAAAGTACAACGAAAAAGAGCTGGCGGATACGAGCAAGTTTTTAAGTTTTGTCCTCCGACACAAACCGGAGGCGATTGGTATCGTACTGGACCGTGAAGGa                                                                                                                                          <  2:281180/289‑1 (MQ=255)
                                                                                                                                                                       gggTATGGGGAAAATGCAAGTATGTTTCTGGCTATGCGTTTTTTGCATAGCGGAAAAATTACGCTGGGTGGAAAAGGCTCTGCGCAGGATTGAGTTGCAGAAAATTCATAAACCAAGTGACAATGACATCAGTAAATAAAAACAGAAAATCCAGGGACGAAAACGAGATGGCAAAGTACAACGAAAAAGAGCTGGCGGATACGAGCAAGTTTTTAAGTTTTGTCCTCCGACACAAACCGGAGGCGATTGGTATCGTACTGGACCGTGAAGGATGGGCGGATATCGATa                                                                                                                          <  2:218816/288‑1 (MQ=255)
                                                                                                                                                                               ggAAAATGCAAGTATGTTTCTGGCTATGCGTTTTTTGCATAGCGGAAAAATTACGCTGTGTGGAAAAGGCTCTGCGCAGGATTGAGTTGCAGAAAATTCATAAACCAAGTGACAATGACATCAGTAAATAAAAACAGAAAATCCAGGGACGAAAACGAGATGGCAAAGTACAACGAAAAAGAGCTGGCGGATACGAGc                                                                                                                                                                                                            <  1:154953/198‑1 (MQ=255)
                                                                                                                                                                               ggAAAATGCAAGTATGTTTCTGGCTATGCGTTTTTTGCATAGCGGAAAAATTACGCTGTGTGGAAAAGGCTCTGCGCAGGATTGAGTTGCAGAAAATTCATAAACCAAGTGACAATGACATCAGTAAATAAAAACAGAAAATCCAGGGACGAAAACGAGATGGCAAAGTACAACGAAAAAGAGCTGGCGGATACGAGc                                                                                                                                                                                                            >  2:154953/1‑198 (MQ=255)
                                                                                                                                                                                  aaaTGCAAGTATGTTTCTGGCTATGCGTTTTTTGCATAGCGGAAAAATTACGCTGTGTGGAAAAGGCTCTGCGCAGGATTGAGTTGCAGAAAATTCATAAACCAAGTGACAATGACATCAGTAAATAAAAACAGAAAATCCAGGGACGAAAACGAGATGGCAAAGTACAACGAAAAAGAGCTGGCGGATACGAGCAAGTTTTTAAGTTTTGTCCTCCGACACAAACCGGAGGCGATTGGTATCGTACTGGACCGTGAAGGATGGGCGGATATCGa                                                                                                                            <  1:442891/275‑1 (MQ=255)
                                                                                                                                                                                  aaaTGCAAGTATGTTTCTGGCTATGCGTTTTTTGCATAGCGGAAAAATTACGCTGTGTGGAAAAGGCTCTGCGCAGGATTGAGTTGCAGAAAATTCATAAACCAAGTGACAATGACATCAGTAAATAAAAACAGAAAATCCAGGGACGAAAACGAGATGGCAAAGTACAACGAAAAAGAGCTGGCGGATACGAGCAAGTTTTTAAGTTTTGTCCTCCGACACAAACCGGAGGCGATTGGTATCGTACTGGACCGTGAAGGATGGGCGGATATCGa                                                                                                                            >  2:442891/1‑275 (MQ=255)
                                                                                                                                                                                               tttCTGGCTATGCGTTTTTTGCATAGCGGAAAAATTACGCTGTGTGGAAAAGGCTCTGCGCAGGATTGAGTTGCAGAAAATTCATAAACCAAGTGACAATGACATCAGTAAATAAAAACAGAAAATCCAGGGACGAAAACGAGATGGCAAAGTACAACGAAAAAGAGCTGGCGGATACGAGCAAGTTTTTAAGTTTTGTCCTCCGACACAAACCGGAGGCGATTGGTATCGTACTGGACCGTGAAGGATGGGCGGATATCGATAAGCTCATACTTTGTGCCCAAAAAGc                                                                                                 >  2:305832/1‑289 (MQ=255)
                                                                                                                                                                                                     gCTATGCGTTTTTTGCATAGCGGAAAAATTACGCTGTGTGGAAAAGGCTCTGCGCAGGATTGAGTTGCAGAAAATTCATAAACCAAGTGACAATGACATCAGTAAATAAAAACAGAAAATCCAGGGACGAAAACGAGATGGCAAAGTACAACGAAAAAGAGCTGGCGGATACGAGCAAGTTTTTAAGTTTTGTCCTCCGACACAAACCGGAGGCGATTGGTATCGTACTGGACCGTGAAGGATGGGCGGATATCGATAAGCTCATACTTTGTGCCCAAAAAGCGGGCaa                                                                                           >  2:493562/1‑289 (MQ=255)
                                                                                                                                                                                                     gCTATGCGTTTTTTGCATAGCGGAAAAATTACGCTGTGTGGAAAAGGCTCTGCGCAGGATTGAGTTGCAGAAAATTCATAAACCAAGTGACAATGACATCAGTAAATAAAAACAGAAAATCCAGGGACGAAAACGAGATGGCAAAGTACAACGAAAAAGAGCTGGCGGATACGAGCAAGTTTTTAAGTTTTGTCCTCCGACACAAACCGGAGGCGATTGGTATCGTACTGGACCGTGAAGGATGGGCGGATATCGATAAGCTCATACTTTGTGCCCAAAAAGCGGGCa                                                                                            <  2:152318/288‑1 (MQ=255)
                                                                                                                                                                                                         tGCGTTTTTTGCATAGCGGAAAAATTACGCTGTGTGGAAAAGGCTCTGCGCAGGATTGAGTTGCAGAAAATTCATAAACCAAGTGACAATGACATCAGTAAATAAAAACAGAAAATCCAGGGACGAAAACGAGATGGCAAAGTACAACGAAAAAGAGCTGGCGGATACGAGCAAGTTTTTAAGTTTTGTCCTCCGACACAAACCGGAGGCGATTGGTa                                                                                                                                                              <  1:474578/218‑1 (MQ=255)
                                                                                                                                                                                                         tGCGTTTTTTGCATAGCGGAAAAATTACGCTGTGTGGAAAAGGCTCTGCGCAGGATTGAGTTGCAGAAAATTCATAAACCAAGTGACAATGACATCAGTAAATAAAAACAGAAAATCCAGGGACGAAAACGAGATGGCAAAGTACAACGAAAAAGAGCTGGCGGATACGAGCAAGTTTTTAAGTTTTGTCCTCCGACACAAACCGGAGGCGATTGGTa                                                                                                                                                              >  2:474578/1‑218 (MQ=255)
                                                                                                                                                                                                          tcGTTTTTTGCATAGCGGAAAAATTACGCTGTGTGGAAAAGGCTCTGCGCAGGATTGAGTTGCAGAAAATTCATAAACCAAGTGACAATGACATCAGTAAATAAAAACAGAAAATCCAGGGACGAAAACGAGATGGCAAAGTACAACGAAAAAGAGCTGGCGGATACGAGCAAGTTTTTAAGTTTTGTCCTCCGACACAAACCGGAGGCGATTGGTATCGTACTGGACCGTGAAGGATGGGCGGATATCGATAAGCTCATACTTTGTGCCCAAAAAGCGGGCAAGCGGt                                                                                      <  1:493562/288‑1 (MQ=255)
                                                                                                                                                                                                                     aTAGCGGAAAAATTACGCTGTGTGGAAAAGGCTCTGCGCAGGATTGAGTTGCAGAAAATTCATAAACCAAGTGACAATGACATCAGTAAATAAAAACAGAAAATCCAGGGACGAAAACGAGATGGCAAAGTACAACGAAAAAGAGCTGGCGGATACGAGCAAGTTTTTAAGTTTTGTCCTCCGACACAAACCGGAGGCGATTGGTATCGTACTGGACCGTGAAGGATGGGCGGATATCGATAAGCTCATACTTTGTGCCCAAAAAGCGGGCAAGCGGTTAACCCGTGCa                                                                           <  1:13278/289‑1 (MQ=255)
                                                                                                                                                                                                                     aTAACGGAAAAATTACGCTGTGTGGAAAAGGCTCTGCGCAGGATTGAGTTGCAGAAAATTCATAAACCAAGTGACAATGACATCAGTAAATAAAAACAGAAAATCCAGGGACGAAAACGAGATGGCAAAGTACAACGAAAAAGAGCTGGCGGATACGAGCAAGTTTTTAAGTTTTGTCCTCCGACACAAACCGGAGGCGATTGGTATCGTACTGGACCGTGAAGGATGGGCGGATATCGATAAGCTCATACTTTGTGCCCAAAAAGCGGGCAAGCGGTTAACCCGTGCa                                                                           <  1:238872/289‑1 (MQ=255)
                                                                                                                                                                                                                        gCGGAAAAATTACGCTGTGTGGAAAAGGCTCTGCGCAGGATTGAGTTGCAGAAAATTCATAAACCAAGTGACAATGACATCAGTAAATAAAAACAGAAAATCCAGGGACGAAAACGAGATGGCAAAGTACAACGAAAAAGAGCTGGCGGATACGAGCAAGTTTTTAAGTTTTGTCCTCCGACACAAACCGGAGGCGAt                                                                                                                                                                   <  1:277349/198‑1 (MQ=255)
                                                                                                                                                                                                                        gCGGAAAAATTACGCTGTGTGGAAAAGGCTCTGCGCAGGATTGAGTTGCAGAAAATTCATAAACCAAGTGACAATGACATCAGTAAATAAAAACAGAAAATCCAGGGACGAAAACGAGATGGCAAAGTACAACGAAAAAGAGCTGGCGGATACGAGCAAGTTTTTAAGTTTTGTCCTCCGACACAAACCGGAGGCGAt                                                                                                                                                                   >  2:277349/1‑198 (MQ=255)
                                                                                                                                                                                                                                   aCGCTGTGTGGAAAAGGCTCTGCGCAGGATTGAGTTGCAGAAAATTCATAAACCAAGTGACAATGACATCAGTAAATAAAAACAGAAAATCCAGGGACGAAAACGAGATGGCAAAGTACAACGAAAAAGAGCTGGCGGATACGAGCAAGTTTTTAAGTTTTGTCCTCCGACACAAACCGGAGGCGATTGGTATCGTACTGGACCGTGAAGGATGGGCGGATATCGATAAGCTCATACTTTGTGCCCAAAAAGCGGGCAAGCGGTTAACCCGTGCa                                                                           <  2:13575/275‑1 (MQ=255)
                                                                                                                                                                                                                                   aCGCTGTGTGGAAAAGGCTCTGCGCAGGATTGAGTTGCAGAAAATTCATAAACCAAGTGACAATGACATCAGTAAATAAAAACAGAAAATCCAGGGACGAAAACGAGATGGCAAAGTACAACGAAAAAGAGCTGGCGGATACGAGCAAGTTTTTAAGTTTTGTCCTCCGACACAAACCGGAGGCGATTGGTATCGTACTGGACCGTGAAGGATGGGCGGATATCGATAAGCTCATACTTTGTGCCCAAAAAGCGGGCAAGCGGTTAACCCGTGCa                                                                           >  1:13575/1‑275 (MQ=255)
                                                                                                                                                                                                                                         tgtgGAAAAGGCTCTGCGCAGGATTGAGTTGCAGAAAATTCATAAACCAAGTGACAATGACATCAGTAAATAAAAACAGAAAATCCAGGGACGAAAACGAGATGGCAAAGTACAACGAAAAAGAGCTGGCGGATACGAGCAAGTTTTTAAGTTTTGTCCTCCGACACAAACCGGAGGCGATTGGTATCGTACTGGACCGTGAAGGATGGGCGGATATCGATAAGCTCATACTTTGTGCCCAAAAAGCGGGCAAGCGGTTAACCCGTGCATTGCTGGATACTGTGGTTg                                                        >  2:335666/1‑288 (MQ=255)
                                                                                                                                                                                                                                                   gCTCTGCGCAGGATTGAGTTGCAGAAAATTCATAAACCAAGTGACAATGACATCAGTAAATAAAAACAGAAAATCCAGGGACGAAAACGAGATGGCAAAGTACAACGAAAAAGAGCTGGCGGATACGAGCAAGTTTTTAAGTTTTGTCCTCCGACACAAACCGGAGGCGATTGGTATCGTACTGGACCGTGAAGGATGGGCGGATATCGa                                                                                                                            >  2:236357/1‑210 (MQ=255)
                                                                                                                                                                                                                                                   gCTCTGCGCAGGATTGAGTTGCAGAAAATTCATAAACCAAGTGACAATGACATCAGTAAATAAAAACAGAAAATCCAGGGACGAAAACGAGATGGCAAAGTACAACGAAAAAGAGCTGGCGGATACGAGCAAGTTTTTAAGTTTTGTCCTCCGACACAAACCGGAGGCGATTGGTATCGTACTGGACCGTGAAGGATGGGCGGATATCGa                                                                                                                            <  1:236357/210‑1 (MQ=255)
                                                                                                                                                                                                                                                   gCTCTGCGCAGGATTGAGTTGCAGAAAATTCATAAACCAAGTGACAATGACATCAGTAAATAAAAACAGAAAATCCAGGGACGAAAACGAGATGGCAAAGTACAACGAAAAAGAGCTGGCGGATACGAGCAAGTTTTTAAGTTTTGTCCTCCGACACAAACCGGAGGCGATTGGTATCGTACTGGACCGTGAAGGATGGGCGGATATCGATAAGCTCATACTTTGTGCCCAAAAAGCGGGCAAGCGGTTAACCCGTGCATTGCTGGATACTGTGGTTGCCACCAGCGAt                                             >  1:314427/1‑289 (MQ=255)
                                                                                                                                                                                                                                                   gCTCTGCGCAGGATTGAGTTGCAGAAAATTCATAAACCAAGTGACAATGACATCAGTAAATAAAAACAGAAAATCCAGGGACGAAAACGAGATGGCAAAGTACAACGAAAAAGAGCTGGCGGATACGAGCAAGTTTTTAAGTTTTGTCCTCCGACACAAACCGGAGGCGATTGGTATCGTACTGGACCGTGAAGGATGGGCGGATATCGATAAGCTCATACTTTGTGCCCAAAAAGCGGGCAAGCGGTTAACCCGTGCATTGCTGGATACTGTGGTTGCCACCAGCGAt                                             >  2:84622/1‑289 (MQ=255)
                                                                                                                                                                                                                                                   gCTCTGCGCAGGATTGAGTTGCAGAAAATTCATAAACCAAGTGACAATGACATCAGTAAATAAAAACAGAAAATCCAGGGACGAAAACGAGATGGCAAAGTACAACGAAAAAGAGCTGGCGGATACGAGCAAGTTTTTAAGTTTTGTCCTCCGACACAAACCGGAGGCGATTGGTATCGTACTGGACCGTGAAGGATGGGCGGATATCGATAAGCTCATACTTTGTGCCCAAAAAGCGGGCAAGCGGTTAACCCGTGCATTGCTGGATACTGTGGTTGCCACCAGCGAt                                             >  1:235292/1‑289 (MQ=255)
                                                                                                                                                                                                                                                     tctGCGCAGGATTGAGTTGCAGAAAATTCATAAACCAAGTGACAATGACATCAGTAAATAAAAACAGAAAATCCAGGGACGAAAACGAGATGGCAAAGTACAACGAAAAAGAGCTGGCGGATACGAGCAAGTTTTTAAGTTTTGTCCTCCGACACAAACCGGAGGCGATTGGTATCGTACTGGACCGTGAAGGATGGGCGGATATCGATAAGCTCATACTTTGTGCCCAAAAAGCGGGCAAGCGGTTAACCCGTGCATTGCTGGATACTGTGGTTGCCACCAGCGATaa                                           >  1:455538/1‑289 (MQ=255)
                                                                                                                                                                                                                                                     tctGCGCAGGATTGAGTTGCAGAAAATTCATAAACCAAGTGACAATGACATCAGTAAATAAAAACAGAAAATCCAGGGACGAAAACGAGATGGCAAAGTACAACGAAAAAGAGCTGGCGGATACGAGCAAGTTTTTAAGTTTTGTCCTCCGACACAAACCGGAGGCGATTGGTATCGTACTGGACCGTGAAGGATGGGCGGATATCGAGAAGCCCATACTTTTTGCCCAAAAAGGGGGCAAGCTGTTAAACCGTGCATTGCTGGATACCGTGGTTTCCACCAGCGATa                                            >  1:502255/1‑288 (MQ=255)
                                                                                                                                                                                                                                                      ctGCGCAGGATTGAGTTGCAGAAAATTCATAAACCAAGTGACAATGACATCAGTAAATAAAAACAGAAAATCCAGGGACGAAAACGAGATGGCAAAGTACAACGAAAAAGAGCTGGCGGATACGAGCAAGTTTTTAAGTTTTGTCCTCCGACACAAACCGGAGGCGATTGGTATCGTACTGGACCGTGAAGGATGGGCGGATATCGATAAGCTCATACTTTGTGCCCAAAAAGCGGGCAAGCGGTTAACCCGTGCATTGCTGGATACTGTGGTTGCCACCAGCGATaaa                                          <  2:6048/289‑1 (MQ=255)
                                                                                                                                                                                                                                                        gcgcAGGATTGAGTTGCAGAAAATTCATAAACCAAGTGACAATGACATCAGTAAATAAAAACAGAAAATCCAGGGACGAAAACGAGATGGCAAAGTACAACGAAAAAGAGCTGGCGGATACGAGCAAGTTTTTAAGTTTTGTCCTCCGACACAAACCGGAGGCGATTGGTATCGTACTGGACCGTGAAGGATGGGCGGATATCGa                                                                                                                            <  2:449822/205‑1 (MQ=255)
                                                                                                                                                                                                                                                        gcgcAGGATTGAGTTGCAGAAAATTCATAAACCAAGTGACAATGACATCAGTAAATAAAAACAGAAAATCCAGGGACGAAAACGAGATGGCAAAGTACAACGAAAAAGAGCTGGCGGATACGAGCAAGTTTTTAAGTTTTGTCCTCCGACACAAACCGGAGGCGATTGGTATCGTACTGGACCGTGAAGGATGGGCGGATATCGa                                                                                                                            >  1:449822/1‑205 (MQ=255)
                                                                                                                                                                                                                                                                      tGCAGAAAATTCATAAACCAAGTGACAATGACATCAGTAAATAAAAACAGAAAATCCAGGGACGAAAACGAGATGGCAAAGTACAACGAAAAAGAGCTGGCGGATACGAGCAAGTTTTTAAGTTTTGTCCTCCGACACAAACCGGAGGCGATTGGTATCGTACTGGACCGTGAAGGATGGGCGGATATCGATAAGCTCATACTTTGTGCCCAAAAAGCGGGCAAGCGGTTAACCCGTGCATTGCTGGATACTGTGGTTGCCACCAGCGATAAAAAGCGTTTTAGTTAtt                          <  1:335666/289‑1 (MQ=255)
                                                                                                                                                                                                                                                                        cAGAAAATTCATAAACCAAGTGACAATGACATCAGTAAATAAAAACAGAAAATCCAGGGACGAAAACGAGATGGCAAAGTACAACGAAAAAGAGCTGGCGGATACGAGCAAGTTTTTAAGTTTTGTCCTCCGACACAAACCGGAGGCGATTGGTATCGTACTGGACCGTGAAGGATGGGCGGATATCGATAAGCTCATACTTTGTGCCCAAAAAGCGGGCAAGCGGTTAACCCGTGCATTGCTGGATACTGTGGTTGCCACCAGCGATAAAAAGCGTTTTAGTTATTcc                        >  2:91723/1‑289 (MQ=255)
                                                                                                                                                                                                                                                                         aGAAAATTCATAAACCAAGTGACAATGACATCAGTAAATAAAAACAGAAAATCCAGGGACGAAAACGAGATGGCAAAGTACAACGAAAAAGAGCTGGCGGATACGAGCAAGTTTTTAAGTTTTGTCCTCCGACACAAACCGGAGGCGATTGGTATCGTACTGGACCGTGAAGGATGGGCGGATATCGATAAGCTCATACTTTGTGCCCAAAAAGCGGGCAAGCGGTTAACCCGTGCATTGCTGGATACTGTGGTTGCCACCAGCGATAAAAAGCGTTTTAGTTATTCCa                       <  1:91723/289‑1 (MQ=255)
                                                                                                                                                                                                                                                                           aaaaTTCATAAACCAAGTGACAATGACATCAGTAAATAAAAACAGAAAATCCAGGGACGAAAACGAGATGGCAAAGTACAACGAAAAAGAGCTGGCGGATACGAGCAAGTTTTTAAGTTTTGTCCTCCGACACAAACCGGAGGCGATTGGTATCGTACTGGACCGTGAAGGATGGGCGGATATCGATAAGCTCATACTTTGTGCCCAAAAAGCGGGCAAGCGGTTAACCCGTGCATTGCTGGATACTGTGGTTGCCACCAGCGATAAAAAGCGTTTTAGTTATTCCAGt                     <  1:84622/289‑1 (MQ=255)
                                                                                                                                                                                                                                                                                              aCAATGACATCAGTAAATAAAAACAGAAAATCCAGGGACGAAAACGAGATGGCAAAGTACAACGAAAAAGAGCTGGCGGATACGAGCAAGTTTTTAAGTTTTGTCCTCCGACACAAACCGGAGGCGATTGGTATCGTACTGGACCGTGAAGGATGGGCGGATATCGATAAGCTCATACTTTGTGCCCAAAAAGCGGGCAAGCGGTTAACCCGTGCATTGCTGGATACTGTGGTTGCCACCAGCGATAAAAAGCGTTTTAGTTATTCCAGTGATGGTAGGTGCATCcgcg  <  2:481132/289‑1 (MQ=255)
                                                                                                                                                                                                                                                                                              aCAATGACATCAGTAAATAAAAACAGAAAATCCAGGGACGAAAACGAGATGGCAAAGTACAACGAAAAAGAGCTGGCGGATACGAGCAAGTTTTTAAGTTTTGTCCTCCGACACAAACCGGAGGCGATTGGTATCGTACTGGACCGTGAAGGATGGGCGGATATCGATAAGCTCATACTTTGTGCCCAAAAAGCGGGCAAGCGGTTAACCCGTGCATTGCTGGATACTGTGGTTGCCACCAGCGATAAAAAGCGTTTTAGTTATTCCAGTGATGGTAGGTGCATCcgcg  <  2:314427/289‑1 (MQ=255)
                                                                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                                                                
TGGTGAGAAAAAAGGCCAGATACCCTTTAATGCCCACTTTTTCTGTCGCCAGCTCCCCGGCTACAGCATCCCCTTGTTGTGTCATAACAATCCCCATTGTTTTTTTGTGTTTGTGTGTTGCTTGAGCCAGGAAAATTCCTGGAACTGAAAAACCTTATCAGCCATGTGGGTATGGGGAAAATGCAAGTATGTTTCTGGCTATGCGTTTTTTGCATAGCGGAAAAATTACGCTGTGTGGAAAAGGCTCTGCGCAGGATTGAGTTGCAGAAAATTCATAAACCAAGTGACAATGACATCAGTAAATAAAAACAGAAAATCCAGGGACGAAAACGAGATGGCAAAGTACAACGAAAAAGAGCTGGCGGATACGAGCAAGTTTTTAAGTTTTGTCCTCCGACACAAACCGGAGGCGATTGGTATCGTACTGGACCGTGAAGGATGGGCGGATATCGATAAGCTCATACTTTGTGCCCAAAAAGCGGGCAAGCGGTTAACCCGTGCATTGCTGGATACTGTGGTTGCCACCAGCGATAAAAAGCGTTTTAGTTATTCCAGTGATGGTAGGTGCATCCGCG  >  NC_000913/4560596‑4561170

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: