Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,206,438 G→A 19.1% intergenic (‑182/‑25) ttdR ← / → ttdA transcriptional activator of ttdABT/L‑tartrate dehydratase, alpha subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,206,4380GA19.1% 130.2 / 10.4 68intergenic (‑182/‑25)ttdR/ttdAtranscriptional activator of ttdABT/L‑tartrate dehydratase, alpha subunit
Reads supporting (aligned to +/- strand):  ref base G (16/37);  new base A (5/8);  total (21/47)
Fisher's exact test for biased strand distribution p-value = 7.41e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ACGATTTCTACCAGTACCTGCAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTG  >  NC_000913/3206204‑3206670
                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                         
aCGATTTCTACCAGTACCTGCAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACa                                                                                                                                                                                                                                        <  2:26708/238‑1 (MQ=255)
    tttCTACCAGTACCTGCAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATAc                                                                                                                                                                                                                                   <  2:665510/239‑1 (MQ=255)
         aCCAGTACCTGCAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGGAGGATTTTTTCCAACAAATaacaaa                                                                                                                                                                                                                               >  2:53372/1‑234 (MQ=255)
              tACCTGCAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCCGAAAAATACAAAAAATggg                                                                                                                                                                                                                         >  1:586948/1‑238 (MQ=255)
               aCCTGGAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAg                                                                                                                                                                                                                        <  2:596603/239‑1 (MQ=255)
               aCCTGCAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTTCTCTGGCGCAAGATTCATTCTTATTTACGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATACAAAACCACATGTTTAGTGGTAGTTTGTGGCTGTGAATTTTTCCAGACAAATACAAAAACTGGAg                                                                                                                                                                                                                        <  2:137795/239‑1 (MQ=255)
               aCCTGCAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAg                                                                                                                                                                                                                        <  1:923934/239‑1 (MQ=255)
               aCCTGCAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAg                                                                                                                                                                                                                        <  1:553889/239‑1 (MQ=255)
                    cAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGAGAATTTTTCCCGACAAATACAAAAACTGGAgattc                                                                                                                                                                                                                    >  1:756761/1‑234 (MQ=255)
                        tCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAAATTTTTCAGACAAATAAAAAAACTTGAGTTGTCctga                                                                                                                                                                                                               >  1:497370/1‑239 (MQ=255)
                          tttGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCatgatg                                                                                                                                                                                                             >  1:312911/1‑239 (MQ=255)
                               cTAAAGGCCAGCTATTCAACATCGTTAATTATCCGCAGTTGTGATAAGCGCTGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCg                                                                                                                                                                                                         <  2:863546/238‑1 (MQ=255)
                                tAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGa                                                                                                                                                                                                        <  1:457267/238‑1 (MQ=255)
                                tAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGa                                                                                                                                                                                                        <  2:312911/238‑1 (MQ=255)
                                tAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGa                                                                                                                                                                                                        <  2:129307/238‑1 (MQ=255)
                                tAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGa                                                                                                                                                                                                        <  1:1050312/238‑1 (MQ=255)
                                tAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACAGTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGa                                                                                                                                                                                                        <  1:256212/238‑1 (MQ=255)
                                       cAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGtaat                                                                                                                                                                                                 <  1:468824/238‑1 (MQ=255)
                                                   aTCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAgg                                                                                                                                                                                       <  2:24839/236‑1 (MQ=255)
                                                   aTCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAgg                                                                                                                                                                                       <  2:1116517/236‑1 (MQ=255)
                                                   aTCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACAATTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAgg                                                                                                                                                                                       <  1:9159/236‑1 (MQ=255)
                                                    tCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTTAGACAAATACAAAAAATGGGGTTGCCATGATTAAGGGAAGGAATAAACAAAAGGCAGt                                                                                                                                                                                   >  1:184062/1‑239 (MQ=255)
                                                    tCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGGTAAGTTTTCTCGTCAAAAAAAAAAAATGGAGGTGGCATGAGAGGCGGAAATAATAAACaaaacagaag                                                                                                                                                                                   >  2:943734/1‑231 (MQ=255)
                                                       ttAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTTGATTTTTTCCGACAAAAACAAAAAATAGAGGTGCCATTGTTGGCGGAAATAAAAAACAACAAGCacggga                                                                                                                                                                                >  1:1095243/1‑234 (MQ=255)
                                                                             aGCGCAGTGTATTACGCAAAACATTGCCATTCCCTGCTCTGCCGCATTATTCATTCTTATTTTCGTTTTGATTCACGAACTGTTAATTAAATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGGGGCGGGGAATTTTTCCAGACAAAGACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGAGTGTCGCt                                                                                                                                                         <  2:302195/239‑1 (MQ=255)
                                                                                      tATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGaa                                                                                                                                                                               <  1:331930/208‑1 (MQ=255)
                                                                                         ttCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAAATTTTTCCGACAAATACAAAAAATGGAGTTGTCCTGATGAGAGAAAGGAAAAAACAACAAGCAGGGAATAAGTTTACAGAGATTGTTGCTAACTTTAACGc                                                                                                                                              >  1:906499/1‑238 (MQ=255)
                                                                                          gccaaaaaatttGCCATTCCATTCTGTGGCGAAAAATTCATTTTTAGTCTGGTGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCCCTATTTTAGTGAAAGTTAGTGGGGGGAAATTTTCCAATACAAATACAAAATGTGTAGTTGCGTAAATGGGCAAAAGTAATAAGAAACAGGAGGATTCTAAGTTGTGAGGATTTTTCGTTAACTTTACCGCCa                                                                                                                                            <  2:586948/229‑1 (MQ=255)
                                                                                                     tGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGGGAAATTTTTCAGAAAAAAAAAAAAAAATGAGGTTGCATGATTAGGGAAAGTAATAAACAACACACAGAGAAAAAAGTTACAGAGATTTTTTCTTACCTTTCCGCCATGAttttcta                                                                                                                                  >  2:60242/1‑234 (MQ=255)
                                                                                                      gCCATCCCCTGCCCTGGCGCAATATTCATTCTTATTTTCGCTTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGTTAGTTTGTGGCGGTGAATTTTTCCAGACAAATAAAAAAACGGGAGTTGCAATGATGAGAGAAAGTAATAAGAAACAGGCAGTAAATAAGTTGAAAGAGATTGTCGCTAATTTTACCGCCATGATTTCTAccc                                                                                                                                <  1:1142186/239‑1 (MQ=255)
                                                                                                          tcccttGCTCTGGCGCATTATTCAGTCTTATTTTCGCGTTGATTCACGACCTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCATATTTTTAGTGGTTTGTTTTGGCGGTGATTTTTTCAAGAAAAATACAAAACTGGGAGTTGCCATGAGGTGCAAAATTAATAAGCAACAGGAAGTGAATAAGTGGACAGAGATTTCCGATAACTTTACCGCCATGATTTCTACCCGAAt                                                                                                                            <  2:1106716/234‑1 (MQ=255)
                                                                                                            cccTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAATAAAAAAAATGGAGGTGACATTATTGGCGGAAGGAATAAGGAAACAGCAGGGAATAAGTTGAGAAAGGTTGTTGCCTACTTTATCGCCCCGAttttttacccaatg                                                                                                                           >  1:1109367/1‑227 (MQ=255)
                                                                                                             ccggCTCTGGCTAAAATTTCATTCTTATTTCCGATTTAATTAACGAAGTTTTAATTAAATATTCACGGTGGAAAAAAATAAAAAACCACATTTTTAGTGGTATTTTGTGGTGTTAAATTTTTCAAGACAAATAAAAAAACGGGAGTTGCCATGATGGGCTAAAGTAATAAGCAACAGCCATTGAATAAGTTAACAGGGATTGTCGTTAACTTTACCGCCATGATTTCTACCCGAATGcc                                                                                                                         <  2:497370/236‑1 (MQ=255)
                                                                                                                            tatTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGAAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGGGGTGAATTTTTCCAGAAAAATACAAAAACTGGAGTTGCCATGATGAGAGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGa                                                                                                          <  1:943734/239‑1 (MQ=255)
                                                                                                                                cattcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAAATTTTTCAAACAAATACAAAAAAAAGAGGTGCCATGATGAGCGAAAGGAATAAAAAACAGGCAGAGAATAAGTTGAAAGAGATTGTCGCTAACTTTACCGCCAATATTTCTAACCCAATGCCTGATGACGCGGGGGGTaaa                                                                                                      >  1:719784/1‑239 (MQ=255)
                                                                                                                                     ttATTTTCGCGTTGATTCACGAACTTTTAATTACATATTCACGGTGGAAAAAAATAAAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGAAAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTaaa                                                                                                 <  1:60242/239‑1 (MQ=255)
                                                                                                                                     ttATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGAAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTCCCAGACAAATACAAAAACGGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAATTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTaaa                                                                                                 <  1:353221/239‑1 (MQ=255)
                                                                                                                                      tATTTTCGCGTTGATTCACGAATTTTTAATTACATATTCACGGTGGCAAAAAATAAAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAAAAAGCAACAGGCAGTGAATAAGTTGACAGAGATTTTCGCTAATTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTaaaa                                                                                                <  1:87828/239‑1 (MQ=255)
                                                                                                                                      tATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAAATTTTTCAGACAAATAAAAAAAATGGAGGTGGCATGATGAGCGGAAGGAATAAGCAAAAAGGAGAGAATAAGATGAAAGAGATTTTGGCTAAATTTTACGCCCTGATTTCTTACCCAAAGCCTGAGTAAGGGGGGGGAAAACTaaaa                                                                                                >  1:964194/1‑239 (MQ=255)
                                                                                                                                      tATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGAGAATTTTTTCAAGCAAAAATAAAAACTGGAGTTGCCGTGATGGGGGAAAGGAATAAACAAAAAGAAGGGAAATAAATGGAAGAAATTTTCGCTTAATTTACCCCCCTGATTTTTTCCCGGAAGACTGATTACGTTGGGGGTAAACAaaaa                                                                                                >  1:142788/1‑239 (MQ=255)
                                                                                                                                      aatttcggtcttGCTTCCCAAATTGTAAATTACATTTTCCGGGTGGAAAAAATAAAAAAACCACTTTTTTAGGGGTAGTTTGGGGCGGTGAATTTTTCCAGACAAATAAAAAACCGGGAGTTGCCATGATGAGCGAAATTAATAAGCAACAGGCAGTAAATAAGTTGACAGAGATTGTCGCAAATTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTaaaa                                                                                                <  2:780063/229‑1 (MQ=255)
                                                                                                                                                 tGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGAAAAATACAAAAACTGGAGTTGCAATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGa                                                                                                          <  1:57911/218‑1 (MQ=255)
                                                                                                                                                 tGATTCACAAACTGTTAATTACATATTCACGGTGGAAAAAAAAAAAAAACCACATTTTTAGTGTTATTTTGTGGCGGTAATTTTTTCCAAACAAATACAAAAACTGGAGTTGCCATAATGAGCAAAAGTAATAAGCAACGGGGAGTAAATAAGTTAACAGAGTTTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGa                                                                                     <  1:193005/239‑1 (MQ=255)
                                                                                                                                                        caaaCTGTGAATTACATATTCACGGCGGAAAAAAAAAAAAAACCACATTTTTAGTGGTAGTTGGTGGCGGGGAATTTTTCAAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAATTAATAAACAACAGGCAGTAAATAGGTCGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGaa                                                                              <  1:859525/237‑1 (MQ=255)
                                                                                                                                                            cttttAATTGCATTTTCCGGGTGGAAAAAAAAAAAAACCCACATTTTGTGGGGTAGTGTGGGGGGGTGATTTTTTCCAGAAAAAAAAAAAAACGTGAGTGGCCATGAGGAGCGAAAGTAATAAGCAAAAGGCAGTGAATAAGTTGACAGAGATTGTCGTTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAAcgt                                                                          <  2:906499/236‑1 (MQ=255)
                                                                                                                                                              gTTAATTACATATTCACGGGAAAAAAAAATATAAAACCACATTTTTGTGGGTAGTTGTTGGCGGTTAATTTTTCAAAACAAATACAAAAACTGGAGTTGCCAGGTGGAGCAAAAATAAAAAGCACTAGGCGTTAAATAAGTGGAAAGAGATTGTCGTTATCTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAAcgtcg                                                                        <  2:964194/239‑1 (MQ=255)
                                                                                                                                                               ttAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGAAAAAAAAAAAAAATGGAGGTGCCATGATGAGCGAAAGTAATAAACAACAAGCAGTGAATAAAGTTACAGAGATTGTCGCTAACTTTACCGCCATGATTTTTTACCGAATGCATGTTGACGTGGTGGGTAAAATAAAAAAACTAAAAGATGGCGCAAcgtcg                                                                        >  1:925507/1‑238 (MQ=255)
                                                                                                                                                                 aaTGACATGTTCCCGGTGGAAAAAAATAAAAAACCACATTTTAGGGTGTAGTTTGGGACGGTGAATTTTCCCAAAAAAATAAAAAAACTGGAGTTCCAGTAATGCGCAAAAGTAAAAACAAACGGGCAGTAAATAATTGGACAGAGTTTGCCTCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGtcgtcg                                                                     <  2:142788/239‑1 (MQ=255)
                                                                                                                                                                   ttACATATTCGCGGGGGAAAAAAATAAAAAACCACATTTGTATTGGTGTTTTGTGGCGGTGAATTTTTCCAAAAAAAAAAAAAACCGGGAGTTTCAATGAAGAGAAAAAGTAAAAACAAAGGGGCAGTAAATAATGGGAAAGAGTTGGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGtcgtcg                                                                     <  2:872854/237‑1 (MQ=255)
                                                                                                                                                                       atatTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAAACAAAAATAAAAAAATGAGTTGTCATTATGGGAGAAAATATAAAAAAACAGGAAGTGAAAAAAGTGACAGAGATTGTCGCTAACTTTACACCCCAGATTTTTACCCACACACCTGTTGACATGGGGGGTGAAATCAAAAAGCTAAAAGATGCGGGAAACTCCTAGATgggg                                                               >  2:392203/1‑239 (MQ=255)
                                                                                                                                                                        tatTCAGGGTGGAAAAAAAAATAAACCCATTTTTTGAGTGGTGGTTTGGGGCGGTCAATTTTGCCAAAAAAAAAAAAAAACGGGAGTTACAATGATGGGAGAAAGTAATAAGCAAGAGGCACTGAAAGCGTGGAAAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGa                                                              <  1:827442/239‑1 (MQ=255)
                                                                                                                                                                        tatTCACGGTGGCAAAAAATATAAACCCACATTTTTAGTGGTAGTTTGTGGCGGTGATTTTTACCAGACAAAAACAAAAACTGGAGTTGCCATGATGAGAGAAAGTAATAAGCAACAGGCAGTAAATAAGTTGAAAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGa                                                              <  1:662820/239‑1 (MQ=255)
                                                                                                                                                                        tatTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGa                                                              <  1:903447/239‑1 (MQ=255)
                                                                                                                                                                         atTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGGGAATTTTTCCACAACAAAAAAAAAAAATGGGTTTGCATGATGAGGAGAAAAAATAAAAAACAGGCAGTGAATAAGATGATAGAGAATGTCGCTAACTTTAACGCCATGATTTCTACCCCAATGGCTGGTGACGGGGGGGGTAAACTCAAAAAAATAAAGGGAGCCGAAAAGGAGTCGGTgggggg                                                             >  2:183559/1‑237 (MQ=255)
                                                                                                                                                                          gacACGGTGGCAAAAAAAAAAAAACAAAATTTTTATGGGTAGTGTGTGGGGGTGAATTTTTCAAGCAAAATAAAAAATCTGGAGTTGCAATGAGGAGAGAAAGTAAAAAGCAACAGGCAGTGAATAAGTTGACAGAGTTTGTCGTTAATTTTTCCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGa                                                              <  2:719784/235‑1 (MQ=255)
                                                                                                                                                                            cACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGAAACAGGCAAGGAATAAGATTACAGAGAATTTCGCTTACTTTACCGCCATGATTTTTACCCGAAAGCCCGATGAGGTGGGGGATAAACTAAAAAAACTAAAAGAAGCCCGAAAGTCGTCGATGGGGGAAAt                                                          >  1:871285/1‑239 (MQ=255)
                                                                                                                                                                                tggaaaaaaaaTAAAAAAACCATTTGTTGTTGGTGGTGTGTGGGGGTAAATTTTTCGAAACAAAAACAAAAACTGGAGTTACTATGATGGGCGAAATAAATAAGCAACAGGAAGTTAATAAGTTCATACAGATTGTCGCTAATTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACTTCGTCGATGGGGAAAATTAt                                                       <  2:174226/233‑1 (MQ=255)
                                                                                                                                                                                 tGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCCGAAAAAAAAAAAAAATTGAGTTGCCCTGATGAGCGGAAGGAATAAACAAAAGGCCGTGAATAAGTTGACAGAGATTTTCGCTAACTTTACCGCCATGATTTTTTCCCGCAAGCCTGATGAAGGGGGGGGTAAACTAAAAAAGCTAAAGGATGCCGGAAAGTCGTCGCTGGGGGAAATTATCt                                                     >  1:832264/1‑239 (MQ=255)
                                                                                                                                                                                   gaaaaaaaaaaaaaCCCACATTTTTAGTGGTAGTTTGGGGCGGTGAATTTTTCAAGAAAAATAAAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGGAAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTa                                                    <  1:1111938/236‑1 (MQ=255)
                                                                                                                                                                                   gCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAAAAAAAAAAATGGAGTTGCCATGATGAGCGGAAGTAATAAACAACAGGCAGTGAATAAAATTACAGAGATTGTAGCTAACTTTAACGCCATGATTTCTTCCCGCATGCCTGATGACGGGGTGGGTAAAATAAAAAAGCTAAAAGAAGGCGAAACGTCGTCGGTGGGGGAAATtatata                                                    >  1:1148268/1‑238 (MQ=255)
                                                                                                                                                                                           tataAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATggg                                                                <  1:691234/218‑1 (MQ=255)
                                                                                                                                                                                             taAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAATACAAAAAATGGAGTTGCCATGATGAGCGAAAGTAATAAACAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCCAAAGCCTGATGACGGGGGGGGTAAAATAAAAAAGCTAAAGGATGCCGAAACGTCGTCGATGGGGGAAAATATTTACCATACCATGt                                         >  1:258223/1‑239 (MQ=255)
                                                                                                                                                                                                      aTTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAg                                                                                                                                                                                                                        <  1:148343/56‑1 (MQ=255)
                                                                                                                                                                                                                   aGTTTGTGGGGGAGAATTTTTCCAAAAAAATAAAAAAACTGGAGTTGCAATGAGGAACGAAAGAAATAAGCAACAGGCGGTAAAGAAGTGGACAGATATTGTCGTAAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGa                    <  2:493407/239‑1 (MQ=255)
                                                                                                                                                                                                                   aGTTGGTGGAGGTAAATTTGACAACAAAAATACAAAAACTGAAGTTGCCATGATGAGCGAAAGGAATAACAAACAGCCAAGAAATAAGTTGACTGAGATTGTCGCTACCTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGa                    <  2:533339/239‑1 (MQ=255)
                                                                                                                                                                                                                     tttGTGGCGGTGAATTTTTCCAGAAAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGAAATAAGCAACAGGCAGTAAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATa                                               <  1:113038/210‑1 (MQ=255)
                                                                                                                                                                                                                                      tttCCAGACAAATACAAAAACGGGAGTTGCCATGATGAGCGAAAGTAAAAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTcctg  <  1:796851/239‑1 (MQ=255)
                                                                                                                                                                                                                                      gtgccAAACAAAGAAAAAAACTGGAGTTGCCATAATGAGCGAAAGAAAAAAACAACAGGCAGTGAATAAGTTGACAGAGATTGTCGTAAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTcctg  <  2:363770/236‑1 (MQ=255)
                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                         
ACGATTTCTACCAGTACCTGCAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTG  >  NC_000913/3206204‑3206670

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: