| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 649,766 | T→G | 12.4% | N242T (AAC→ACC) | citE ← | citrate lyase, citryl‑ACP lyase (beta) subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 649,766 | 0 | T | G | 12.4% | 189.8 / 3.4 | 75 | N242T (AAC→ACC) | citE | citrate lyase, citryl‑ACP lyase (beta) subunit |
| Reads supporting (aligned to +/- strand): ref base T (14/50); new base G (9/2); total (23/52) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.34e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.29e-01 | |||||||||||
CTACCCGTTCTTGTCGTTGAGATTGTTCAATTTTCTGCGTCATTTTGATTGCCTTATTCTTCGCGGATGCCGGAAAGTTCTGCACGGGAGAGCACCAGACGGGCGCGATCGATAACCGGACCGTCCACCATCTTGCCGTTCAGGGAAACCACGCCGAGGCCTTCGCGAGCGGCGGCTTCAGCGGCTTCTACGACGCGGCGGGCGTGATCCACTTCTTTCT‑GGGTCGGTGCGTAGAGGTTGTGCAGCAGATCAATCTGACGCGGGTTGATCAGCGATTTGCCGTCAAAGCCCAGCTGTTTGATGTGGGCGGCTTCTTGCAGAAATCCGGCTTCGTTGTTAGCGTCGGAATAGACGGTATCGAACGCCTGAATACCCGCAGAGCGCGCGGCCTGCAAAATGGAACAGCGTGCGAACAGCAGTTCAGTTCCTTCCGGGGAGCGTTCTGTACGCAGGTTGCGCACATAGTCTTCTGCACC > NC_000913/649529‑650004 | cTACCCGTTCTTGTCGTTGAGATTGTTCAATTTTCTGCGTCATTTTGATTGCCTTATTCTTCGCGGATGCCGGAAAGTTCTGCACGGGAGAGCACCAGACGGGCGCGATCGATAACCGGACCGTCCACCATCTTGCCGTTCAGGGAAACCACGCCGAGGCCTTCGCGAGCGGCGGCTTCAGCGGCTTCTACGACGCGGCGGGCGTGGTCCCCTTCTTTCT‑GGGTCGGTGCGGAGGGGtt > 1:456222/1‑239 (MQ=255) tttgcGTTGAGATTGTTCAATTTGCTGCGTCATTTTGATTGCCTTATTCTTCGCGGATGCCGGAAAGTTCTGCACGGGAGAGCACCAGACGGGCGCGATCGATAACCGGACCGTCCACCATCTTGCCGTTCAGGGAAACCACGCCGAGGCCTTCGCGAGCGGCGGCTTCAGCGGCTTCTACGACGCGGCGGGCGTGATCCACTTCTTTCT‑GGGTCGGTGCGTAGAGGTTGTGCAGCAGa < 2:833115/235‑1 (MQ=255) tGTCGTTGAGATTGTTCAATTTTCTGCGTCATTTTGATTGCCTTATTCTTCGCGGATGCCGGAAAGTTCTGCACGGGAGAGCACCAGACGGGCGCGATCGATAACCGGACCGTCCACCATCTTGCCGTTCAGGGAAACCACGCCGAGGCCTTCGCGAGCGGCGGCTTCAGCGGCTTCTACGGCGCGGCGGGCGGGGGCCCCTTTTTTCTGGGGCCGGTGCG‑AACGGTTGTgcgggcgga > 2:708947/1‑232 (MQ=255) tgtGAGATTGTTCAAGTTTCTGCGTCATTTTGATTGCCTGTTTATTCGCGGATGCCGGAAAGTTCTGCACGGGAGAGCACCAGACGGGCCGGAGCGATAACCGGACCGGCCACCATCTTGCCGTTCAGGGAAACCACGCCGAGGCCTTAGCGAGCGGCGGCTTCAGCGGCTTCTACGACGCGGCGGGCGTGATCCACTTCTTTCT‑GGGTCGGTGCGTAGAGGTTGTGCAGCAGATCAAt < 2:1133567/237‑1 (MQ=255) gTTGAGATTGTTCAATTTTCTGCGTCATTTTGATTGCCTTATTCTTCGCGGATGCCGGAAAGTTCTGCACGGGAGAGCACCAGACGGGCGCGATCGATAACCGGACCGTCCACCATCTTGCCGTTCAGGGAAACCACGCCGAGGCCTTCGCGAGCGGCGGCTTCAGCGGCTTCTACGACGCGGCGGGCGTGATCCACTTCTTTCT‑GGGTCGGTGCGTAGAGGTTGTGCAGCAGATCAAt < 1:807884/239‑1 (MQ=255) ttGTTCAAGTTTCTGCGTCATTTGGATTGCCTTATTCTTCGCGGATGCCGGAAAGTTCTGCACGGGAGAGCACCAGACGGGCGCGATCGATAACCGGACCGTCCACCATCTTGCCGTTCAGGGAAACCACGCCGAGGCCTTCGCGAGCGGCGGCTTCAGCGGCTTCTACGACGCGGCGGGCGTGATCCACTTCTTTCT‑GGGTCGGTGCGTAGAGGTTGTGCAGCAGATCAATCTGAcgc < 1:961405/239‑1 (MQ=255) ttCAATTTTCTGCGTCATTTTGATTGCCTTATTCTTCGCGGATGCCGGAAAGTTCTGCACGGGAGAGCACCAGACGGGCGCGATCGATAACCGGACCGTCCACCATCTTGCCGTTCAGGGAAACCACGCCGAGGCCTTCGCGAGCGGCGGCTTCAGCGGCTTCTACGACGCGGCGGGCGGGATCCCCTTATTTTC‑GGGGCGGGGCGGAGAGGGTGTGGAGCAGATCAATCTGACGCggg > 1:955461/1‑239 (MQ=255) ttttCTGCGTCATTTTGATTGCCTTATTCTTCGCGGATGCCGGAAAGTTCTGCACGGGAGAGCACCAGACGGGCGCGATCGATAACCGGACCGTCCACCATCTTGCCGTTCAGGGAAACCACGCCGAGGCCTTCGCGAGCGGCGGCTTCAGCGGCTTCTACGACGCGGCGGGCGTGATCCACTTCTTTCT‑GGGTCGGTGCGTAGAGGTTGTGCAGCAGATCAATCTGACGCGGGTTGAt < 2:614311/239‑1 (MQ=255) ttttCTGCGTCATTTTGATTGCCTTATTCTTCGCGGATGCCGGAAAGTTCTGCACGGGAGAGCACCAGACGGGCGCGATCGATAACCGGACCGTCCACCATCTTGCCGTTCAGGGAAACCACGCCGAGGCCTTCGCGAGCGGCGGCTTCAGCGGCTTCTACGACGCGGCGGGCGTGATCCACTTCTTTCT‑GGGTCGGTGCGGAGAGGGTGTGCAGCAGAACCATCTGAAGCGGGTTGa > 1:951786/1‑238 (MQ=255) ttttCTGCGTCATTTTGAGTGCCTCATTCATCGCGGATGCCGGGAAGTTCTGCCCGGGGGAGCACCAGACGGGCGCGATCGATAACCGGACCGTCCACCATCTTGCCGTTCAGGGGAACCACGCCGAGGCCTTCGCGAGCGGCGGCTTCAGCGGCTTCTACGACGCGGCGGGCGTGATCCACTTCTTTCT‑GGGTCGGTGCGTAGAGGTTGTGCAGCAGATCAATCTGACGCGGGTTGAt < 2:1149194/239‑1 (MQ=255) ttGATTGCCTTATTCTTCGCGGATGCCGGAAAGTTCTGCACGGGAGAGCACCAGACGGGCGCGATCGATAACCGGACCGTCCACCATCTTGCCGTTCAGGGAAACCACGCCGAGGCCTTCGCGAGCGGCGGCTTCAGCGGCTTCTACGACGCGGCGGGCGTGATCCACTTCTTTCT‑GGGTCGGTGCGTAGAGGTTGTGCAGCAGATCAATCTGACGCGGGTTGATCAGCGATTTGCCGt < 1:708947/239‑1 (MQ=255) gATTGCCTTATTCTTCGCGGATGCCGGAAAGTTCTGCACGGGAGAGCACCAGACGGGCGCGATCGATAACCGGACCGTCCACCATCTTGCCGTTCAGGGAAACCACGCCGAGGCCTTCGCGAGCGGCGGCTTCAGCGGCTTCTACGACGCGGCGGGCGTGATCCACTTCTTTCT‑GGGTCGGTGCGTAGAGGTTGTGCAGCAGATCAATCTGACGCGGGTTGATCAGCGATTTGCCGTCa < 1:339021/239‑1 (MQ=255) cTTATTCTTCGCGGATGCCAGAAAGTTCTGCACGGGAGAGCACCAGACGGGCGCGATCGATAACCGGACCGTCCACCATCTTGCCGTTCAGGGAAACCACGCCGAGGCCTTCGCGAGCGGCGGCTTCAGCGGCTTCTACGACGCGGCGGGCGTGATCCACTTCTTTCT‑GGGTCGGTGCGTAGAGGTTGTGCAGCAGATCAATCTGACGCGGGTTGATCAGCGATTTGCCGTCAAAGcc < 2:123182/238‑1 (MQ=255) ttATTCTTCGCGGATGCGAGAAAGTTCTGCACGGGAGGGCACCAGACGGGCGCGATCGATAACCGGACCGTCCACCATCTTGCCGTTCAGGGAAACCACGCCGAGGCCTTCGCGAGCAGCGGCTTCAGCGGCTTCTACGACGCGGCGGGCGTGATCCACTTCTTTCT‑GGGTCGGTGCGTAGAGGTTGTGCAGCAGATCAATCTGACGCGGGTTGATCAGCGATTTGCCGACAAAGCCCa < 2:799850/239‑1 (MQ=255) tATTCTTCGCGGATGCCGGAAAGTTCTGCACGGGAGAGCACCAGACGGGCGCGATCGATAACCGGACCGTCCACCATCTTGCCGTTCAGGGAAACCACGCCGAGGCCTTCGCGAGCGGCGGCTTCAGCGGCTTCTACGACGCGGCGGGCGTGATCCACTTCTTTCT‑GGGTCGGTGCGTAGAGGTTGTGCAGCAGATCAATCTGACGCGGGTTGATCAGCGATTTGCCGTCAAAGCCCAg < 1:35577/239‑1 (MQ=255) aTGCCGGAAAGTTCTGCACGGGAGAGCACCAGACGGGCGCGATCGATAACCGGACCGTCCACCATCTTGCCGTTCAGGGAAACCACGCCGAGGCCTTCGCGAGCGGCGGCTTCAGCGGCTTCTACGACGCGGCGGGCGGGATCCACTTCTTTCT‑GGGTCGGTGGGTAGAGGGTGTGCAGCCGGTCAATCTGACGCGGGGTGATCAGCGATTTGCCGTCAAAGCCCAGCTGTTTGAttgg > 1:81322/1‑236 (MQ=255) cGGAAAGTTCTGCACGGGAGAGCACCAGACGGGCGCGATCGATAACCGGACCGTCCACCATCTTGCCGTTCAGGGAAACCACGCCGAGGCCTTCGCGAGCGGCGGCTTCAGCGGCTTCTACGACGCGGCGGGCGTGATCCACTTCTTTCT‑GGGTCGGTGCGTAGAGGTTGTGCAGCAGATCAATCTGACGCGGGTTGATCAGCGATTTGCCGTCAAAGCCCAGCTGTTTGATGTGggcg < 1:967950/239‑1 (MQ=255) tGCACGGGAGAGCACCAGGCGGGCGCGATCGATAACCGGACCGTCCACCATCTTGCCGTTCAGGGAAACCACGCCGAGGCCTTCGCGAGCGGCGGCTTCAGCGGCTTCTACGACGCGGCGGGCGTGATCCACTTCTTTCT‑GGGTCGGTGCGTAGAGGTTGTGCAGCAGATCAATCTGACGCGGGTTGATCAGCGATTTGCCGTCAAAGCCCAGCTGTTTGATGTGGGCGGCTTCTTGCa < 1:1049101/239‑1 (MQ=255) gagCACCGGACGGGGGCGGTCGATGACCGAGCCGTCCACCATCTTGCCGTTCAGGGAAACCACGCCGAGGCCTTCGCGAGCGGCGGCTTCAGCGGCTTCTACGACGCGGCGGGCGTGATCCACTTCTTTCT‑GGGTCGGTGCGTAGAGGTTGTGCAGCAGATCAATCTGACGCGGGTTGATCAGCGATTTGCCGTCAAAGCCCAGCTGTTTGATGTGGGCGGCGTCTTGCAGAAATCCg < 2:507875/238‑1 (MQ=255) cACCAGACGGGCGCGATCGATAACCGGACCGTCCACCATCTTGCCGTTCAGGGAAACCACGCCGAGGCCTTCGCGAGCGGCGGCTTCAGCGGCTTCTACGACGCGGCGGGCGTGATCCCCTTTTTTCT‑GGGGCGGTGCGTAGAGGGTGTGCAGCCGATCCATCTGAAGCGGGTTGATCAGCGATTTGCCGGCAAAGCCCAGCTGTTTGGTGTGGGCGGCTTCTTGCAGAAATCCGGCt > 1:498796/1‑238 (MQ=255) cACCAGACGGGCGCGATCGATAACCGGACCGTCCACCATCTTGCCGTTCAGGGAAACCACGCCGAGGCCTTCGCGAGCGGCGGCTTCAGCGGCTTCTACGACGCGGCGGGCGTGATCCACTTCTTTCT‑GGGTCGGTGGGTAGAGGTTTTGCAGCAGATCAATCTGACGCGGGTTGGTCAGCGATTTGCCGTCAAAGCCCAGCTGTTTGATGTGGGGGGCTTTTTGCAGAAATCCGGCt > 1:181356/1‑238 (MQ=255) gACGGGCGCGATCGATAACCGGACCGTCCACCATCTTGCCGTTCAGGGAAACCACGCCGAGGCCTTCGCGAGCGGCGGCTTCAGCGGCTTCTACGACGCGGCGGGCGTGATCCACTTCTTTCT‑GGGTCGGTGCGTAGAGGTTGTGCAGCAGATCAATCTGACGCGGGTTGATCAGCGATTTGCCGTCAAAGCCCAGCTGTTTGATGTGGGCGGCTTCTTGCAGAAATCCGGCTTCgttg > 1:758377/1‑239 (MQ=255) gACGGGCGCGATCGATAACCGGACCGTCCACCATCTTGCCGTTCAGGGAAACCACGCCGAGGCCTTCGCGAGCGGCGGCTTCAGCGGCTTCTACGACGCGGCGGGCGTGATCCACTTCTTTCT‑GGGTCGGGGCGTAGAGGTTGTGCAGCAGATCAATCTGACGCGGGGTGATCAGCGATTTGCCGTCAAAGCCCAGCTGTTTTATGTGGGCGGCTTCTTGCAGAAATCCGGCTTCgttg > 1:876125/1‑239 (MQ=255) gcgAACGATAAGCGGTCCGTCCAGCGTCTTGCCGTTCAGGGAAACCAGGCCGAGGCCGTGGCGGGCGGCGGCTTCAGCGGCGTCTACGACGCGGCGGGCGTGATCCACTTCTTTCT‑GGGTCGGAGCGTAGAGGTTGTGCAGCAGATCAATCTGACGCGGGTTGATCAGCGATTTGCCGTCAAAGCCCAGCTGTTTGATGTGGGCGGCTTCTTGCAGAAATCCGGCTTCGTTGTTAGCg < 2:916407/238‑1 (MQ=255) ccaatccgtAACCGGACCGCCCACCAACTTGCCGTGCAGGGAAGCCGCGCAGAGGCCTGCGCGAGCGGCGGCTGCAGCGGCTTCTACGACGCGGCGGGCGTGATCCACTTCTTTCT‑GGGTCGGTGCGTAGAGGTTGTGCAGCAGATCAATCTGACGCGGGTTGATCAGCGATTTGCCGTCAAAGCCCAGCTGTTTGATGTGGGCGGCTTCTTGCAGAAAGCCGGCTTCGTTGTTGGCg < 2:281876/230‑1 (MQ=255) tcgatAACCGGACCGTCCACCATCTTGCCGTTCAGGGAAACCACGCCGAGGCCTTCGCGAGCGGCGGCTTCAGCGGCTTCTACGACGCGGCGGGCGTGATCCACTTCTTTCT‑GGGTCGGTGCGTAGAGGTTGTGCAGCAGATCAATCTGACGCGGGTTGATCAGCGATTTGCCGTCAAAGCCCAg < 1:694683/185‑1 (MQ=255) gcgatAGCCGGCCCGGCCACCGGCGTTCCGTTCAGGGAAACCCCGCCGAGGCGTTCGCGAGCGGCGGCGTCAGCGGCGGCTACGACGCGGCGGGCGGGATCCACTTCTGGCT‑GGGGCGGTGCGTAGAGGTTGTGCAGCAGATCAATCTGACGCGGGCTGATGAGCGATTGGCCGTCAAAGCACAGCTGTTGGATGTGGGCGGCTTCGTGCATAAATCCGGGTTCATTGTTAgggcccga < 2:50335/238‑8 (MQ=255) aaCCGGACCGTCCACCATCTTGCCGTTCAGGGAAACCACGCCGAGGCCTTCGCGAGCGGCGGCTTCAGCGGCTTCTACGACGCGGCGGGCGTGATCCACTTCTTTCT‑GGGTCGGTGCGTAGAGGTTGTGCAGCAGATCAATCTGACGCGGGTTGATCAGCGATTTGCCGTCAAAGCCCAGCTGTTTGATGTGGGCGGCTTCTTGCAGAAATCCGGCTTCGTTGTTAGCGTCGGAATAGa < 1:593618/239‑1 (MQ=255) gACCGTCCACCATCTTGCCGTTCAGGGAAACCACGCCGAGGCCTTCGCGAGCGGCGGCTTCCGCGGCTTCTACCACGCGGCGGGCGTGTCCCCCTTCTTTCT‑TGGGCGGTGCGTAGAGGTTGTGCAGACGAACAATCCTTCCGGGGGTGATTCGCGATTTTCCGTCTCAGCCCCCCTGTTTGGTGTGGGGGGCGTCTCGTCGAAAACCTGCTCCGTTGTTAGCGTCGGACTAGACGGtg > 2:1021540/1‑238 (MQ=255) aCCGTCCACCATCTTGCCGTTCAGGGAAACCACGCCGAGGCCTTCGCGAGCGGCGGCTTCAGCGGCTTCTACGACGCGGCGGGCGTGGTCCACCTCTTTCT‑GGGTCGGTGCGGAGAGGGTTTGCCGCAGATCAATCTGCCGCGGGGTTATCCGCGCTTTGCCGTCAAAGACCAGCCGGTTGGTGTGGGGGGGTTCTTGCAGAAATCCGGCTTCGCTGTTAGCCTCCGaaagtaccgta > 2:296854/1‑229 (MQ=255) ccGTCCACCATCTTGCCGTTCAGGGAAACCACGCCGAGGCCTTCGCGAGCGGCGGCTTCAGCGGCTTCTACGACCCGGCGGGCGCGATCCCCTTCTTTCT‑GGGTCGGGGCGGAGAGGGTGTGGAGCCGGACAATCTGACGCGGGTTGATTAACTCTTTTCCGGCAAAGCCCCGATGTTTTATGTTGGGGGCCTCTTTTAGAACTCCCGTTTTCCTGTTATCGCCGGAGTAGACGGTAtg > 2:528463/1‑238 (MQ=255) tCCACCATCTTGCCGTTCAGGGAAACCACGCCGAGGCCTTCGCGAGCGGCGGCTTCAGCGGCTTCTACGGCGCGGCGGGCGGGATCCACTTCTTTCT‑GGGGCGGGGCGTAGAGGGTGTGCAGCAGGTCAATCTGACGCGGGTTGATAAGGCATTTGCCGTCAAAACCCAGCTGGTTTGTGTGGGGGGCTTCTTGCAGGAATCCGGGTTCGTTGTTGGCGGCGGAAAAAACGGGGTCGaa > 2:922937/1‑239 (MQ=255) tCCACCATCTTGCCGTTCAGGGAAACCACGCCGAGGCCTTCGCGAGCGGCGGCTTCAGCGGCTTCTACGACGCGGCGGGCGTGATCCACTTCTTTCT‑GGGTCGGTGCGTAGAGGTTGGGCAGCAGATCAATCTGACGCGGGTTGATCAGCGATTTGCCGTCAAAGCCCAGCTGTTTGATGTGGGCGGCTTCTTGCAGAAATCCGGCTTCGTTGTTAGCGTCGGAATAGACGGTATCGa > 1:917922/1‑238 (MQ=255) ccaccaTCCTGCCGTTCAGGGAACCCACGCCGCGGCCTTCGCGAGCGGCGGCTTCAGCCGCTTCTACGACGCGGCGGGCGTGATCCACTTCTTTCT‑GGGTCGGTGCGTAGAGGTTGTGCAGCAGATCAATCTGACGCGGGTTGATCAGCGATTTGCCGTCAAAGCCCAGCTGTTTGATGTGGGCGGCTTCTTGCAGAAATCCGGCTTCGTTGTTAGCGTCGGAATAGACGGTATCGaa < 2:997791/238‑1 (MQ=255) ccaGCTTGCCGTTCGGGAAACCCCCGCCGAGGCCTTCGCGAGCGCCGGGTTCAGGGGCTGTTACGACGCGGCGGGGGTGATCCACTTCTGTCT‑GGGTCGGGGCGTAGAGGTTGTGCAGCAGATCATTCTGGCGCGGGTTGATCAGCGATTTGCCGTCAAAGCCCAGCTGTTTGATGTGGGCGGCTTCTTCCAGAAATCCGGCTTCGTTGTTAGCGTCGGAATAGCCGGTATCGAACGcc < 2:836867/239‑1 (MQ=255) aTCTTGCCGTTCAGGGAAACCACGCCGAGGCCTTCGCGAGCGGCGGCTTCAGCGGCTTCTACGACGCGGCGGGCGTGATCCACTTCTTTCT‑GGGTCGGTGCGTAGAGGTTGTGCAGCAGATCAATCTGACGCGGGTTGATCAGCGATTTGCCGTCAAAGCCCAGCTGTTTGATGTGGGCGGCTTCTTGCAGAAATCCGGCTTCGTTGTTAGCGTCGGAATAGACGGTATCGAACGCCTg < 1:1021540/239‑1 (MQ=255) tttGCCGTTCAGGGAAGCCGCGCCGGGGCCTTAGCGAGCGGCCGCTTCGGCGGGTTCTACGACGCGGCCGGCGTGATCCACTTCTTTCT‑GGGTCGGTGCGTAGAGGTTGAGCAGCAGATCAATCTGACGCGGGTTGATCATCGAGTTGCCGTCAAAGCCCAGCTGTTTGATGTGGGCGGCTTCTTGCAGAAATCCGGCTCAGTTGTTAGCGTCGGAATAGACGGGATCGAACGCCTGaa < 2:955461/238‑1 (MQ=255) tGCCGTTCAGGGAAACCACGCCGAGGCCTTCGCGAGCGGCGGCTTCAGCGGCTTCTACGACGCGGCGGGCGTGATCCACTTCTTTCT‑GGGTCGGTGCGTAGAGGTTGTGCAGCAGATCAATCTGACGCGGGTTGATCAGCGATTTGCCGTCAAAGCCCAGCTGTTTGATGTGGGCGGCTTCTTGCAGAAATCCGGCTTCGTTGTTAGCGTCGGAATAGACGGTATCGAACGCCTGGAt > 1:781910/1‑238 (MQ=255) tGCCGTTCAGGGAAACCACGCCGAGGCCTTCGCGAGCGGCGGCTTCAGCGGCTTCTACGACGCGGCGGGCGTGATCCACTTCTTTCT‑GGGTCGGTGCGTAGAGGTTGTGCAGCAGATCAATCTGACGCGGGTTGATCAGCGATTTGCCGTCAAAGCCCAGCTGTTTGATGTGGGCGGCTTCTTGCAGAAATCCGGCTTCGTTGTTAGCGTCGGAATAGACGGTATCGAACGCCTGAAt > 1:797128/1‑238 (MQ=255) ttCAGGGAAACCACGCCGAGGCCTTCGCGAGCGGCGGCTTCAGCGGCTTCTACGACGCGGCGGGCGGGGTCCACTTCTTTCT‑GGGGCGGGGGGGAGAGGGTTTGCAGCAGATCAATCTGGCGCGGGTTGGTCCAGGATTTGCCGTCAAAACCCCGGCGTTTTGTGTTGGGGGCTTCTTGCAGGAAATCGGCTTCGGTTTTTGCGGCGGAATAGACGGTATCGGACGCCTGAATAcccc > 2:1129783/1‑237 (MQ=255) cAGGGAAACCCCGGCGAGGTCGTCGGGAGCGGCGGCCTCAGCGGCTTCTACGACGCGGCGGGCGTGATCCACTTCTTTCT‑GGGTCGGTGCGTAGAGGGTGTGCAGCAGATCAATCTGACGCGGGTTGATCAGCGATTTGCCGTCAAAGCCCAGATGATTGATGTGGGCGGCTGCTTGCAGAAATCCGGCTTCGTTGTTAGCGTCGGAATAGACTGTCTCGAACGCCTGAATACCCGCag < 2:336311/239‑1 (MQ=255) cAGGGAAACCACGCCGAGGCCTTCGCGAGCGGCGGCTTCAGCGGCTTCTACGACGCGGCGGGCGTGATCCACTTCTTTCT‑TGGTCGGTGCGTAGAGGGTTTGCCGCAGATCAATCTGGCGCGGGTTGATTAGCGATTTGCCGTCAAAACCCAGCTGTTTGATGTGGGGGGCTTATTGCAGGAATCCGGCTTCTGTGTTATCGTCTGCATAGGCGGTACCGGACGGCTGAATACCCGCag > 2:547627/1‑239 (MQ=255) ccACGCCGAGGCCTTCGCGGACGGCGGCTTCAGCGGCTTCTACGACGCGGCTGGCGTTATCCACTTCTTTCT‑GGGTCGGTGCGTAGAGGTTGTGCAGCAGATCAATCTGACGCGGGTTGATCAGCGATTTGCCGTCAAAGCCCAGCTGTTTGATGTGGGCGGCTTCTTGCAGAAATCCGGCTTCGTTGTTAGCGTCGGAATAGACGGTATCGAACGCCTGAATACCCGCAGAGcgcgcg < 2:1158564/239‑1 (MQ=255) cGAGGCCTTCGCGAGCGGCGGCTTCAGCGGCTTCTACGACGCGGCGGGCGTGATCCACTTCTTTCT‑GGGTCGGTGCGTAGAGGTTGTGCAGCAGATCAATCTGACGCGGGTTGATCAGCGATTTGCCGTCAAAGCCCAGCTGTTTGATGTGGGCGGCTTCTTGCAGAAATCCGGCTTCGTTGTTAGCGTCGGAATAGACGGTATCGAACGCCTGAATACCCGCAGAGCGCGCGGCCTg < 1:219252/238‑1 (MQ=255) cGAGGCCTTCGCGAGCGGCGGCTTCAGCGGCTTCTACGACGCGGCGGGCGTGATCCACTTCTTTCT‑GGGTCGGTGCGTAGAGGTTGTGCAGCAGATCAATCTGACGCGGGTTGATCAGCGATTTGCCGTCAAAGCCCAGCTGTTTGATGTGGGCGGCTTCTTGCAGAAATCCGGCTTCGTTGTTAGCGTCGGAATAGACGGTATCGAACGCCTGAATACCCGCAGAGCGCGCGGCCTg < 1:442791/238‑1 (MQ=255) gcgAGCGGCGGCTTCAGCGGCTTCTACGACGCGGCGGGCGTGATCCACTTCTTTCT‑GGGTCGGTGCGTAGAGGTTGTGCAGCAGATCAATCTGACGCGGGTTGATCAGCGATTTGCCGTCAAAGCCCAGCTGTTTGATGTGGGCGGCTTCTTGCAGAAATCCGGCTTCGTTGTTAGCGTCGGAATAGACGGTATCGAACGCCTGAATACCCGCAGAGCGCGCGGCCTGCAAAATGGAAc < 1:1129783/239‑1 (MQ=255) gcgAGCGGCGGCTTCAGCGGCTTCTACGACGCGGCGGGCGTGATCCACTTCTTTCT‑GGGTCGGTGCGTAGAGGTTGTGCAGCAGATCAATCTGACGCGGGTTGATCAGCGATTTGCCGTCAAAGCCCAGCTGTTTGATGTGGGCGGCTTCTTGCAGAAATCCGGCTTCGTTGTTAGCGTCGGAATAGACGGTATCGAACGCCTGAATACCCGCAGAGCGCGCGGCCTGCAAAATGGAAc < 2:758377/239‑1 (MQ=255) gcgAGCGGCGGCTTCAGCGGCTTCTACGACGCGGCGGGCGTGATCCACTTCTTTCT‑GGGTCGGTGCGTAGAGGTTGTGCAGCAGATCAATCTGACGCGGGTTGATCAGCGATTTGCCGTCAAAGCCCAGCTGTTTGATGTGGGCGGCTTCTTGCAGAAATCCGGCTTCGTTGTTAGCGTCGGAATAGACGGTATCGAACGCCTGAATACCCGCAGAGCGCGCGGCCTGCAAAATGGAAc < 1:982710/239‑1 (MQ=255) gcgAGCGGCGGCTTCAGCGGCTTCTACGACGCGGCGGGCGTGATCCACTTCTTTCT‑GGGTCGGTGCGTAGAGGTTGTGCAGCAGATCAATCTGACGCGGGTTGATCAGCGATTTGCCGTCAAAGCCCAGCTGTTTGATGTGGGCGGCTTCTTGCAGAAATCCGGCTTCGTTGTTAGCGTCGGAATAGACGGTATCGAACGCCTGAATACCCGCAGAGCGCGCGGCCTGCAAAATGGAAc < 1:296854/239‑1 (MQ=255) cggcggCTTCAGCGGCTTCTACGACGCGGCGGGCGTGATCCACTTCTTTCT‑GGGTCGGTGCGTAGAGGTTGTGCAGCAGATCAATCTGACGCGGGTTGATCAGCGATTTGCCGTCAAAGCCCAGCTGTTTGATGTGGGCGGCTTCTTGCAGAAATCCGGCTTCGTTGTTAGCGt > 1:1007728/1‑174 (MQ=255) cggcggCTTCAGCGGCTTCTACGACGCGGCGGGCGTGATCCACTTCTTTCT‑GGGTCGGTGCGTAGAGGTTGTGCAGCAGATCAATCTGACGCGGGTTGATCAGCGATTTGCCGTCAAAGCCCAGCTGTTTGATGTGGGCGGCTTCTTGCAGAAATCCGGCTTCGTTGTTAGCGt < 2:1007728/174‑1 (MQ=255) tCAGCGGCTTCTACGACGCGGCGGGCGTGATCCACTTCTTTCT‑GGGTCGGTGCGTAGAGGTTGTGCAGCAGATCAATCTGACGCGGGTTGATCAGCGATTTGCCGTCAAAGCCCAGCTGTTTGATGTGGGCGGCTTCTTGCAGAAATCCGGCTTCGTTGTTAGCGTCGGAATAGACGGTATCGAACGCCTGAATACCCGCAGAGCGCGCGGCCTGCAAAATGGAACAGCGTGCGAAcag < 1:94540/239‑1 (MQ=255) tCAGCGGCTTCTACGACGCGGCGGGCGTGATCCACTTCTTTCT‑GGGTCGGTGCGTAGAGGTTGTGCAGCAGATCAATCTGACGCGGGTTGATCAGCGATTTGCCGTCAAAGCCCAGCTGTTTGATGTGGGCGGCTTCTTGCAGAAATCCGGCTTCGTTGTTAGCGTCGGAATAGACGGTATCGAACGCCTGAATACCCGCAGAGCGCGCGGCCTGCAAAATGGAACAGCGTGCGAAcag < 1:585612/239‑1 (MQ=255) cAGCGGCTTCTACGACGCGGCGGGCGTGATCCACTTCTTTCT‑GGGTCGGTGCGTAGAGGTTGTGCAGCAGATCAATCTGACGCGGGTTGATCAGCGATTTGCCGTCAAAGCCCAGCTGTTTGATGTGGGCGGCTTCTTGCAGAAATCCGGCTTCGTTGTTAGCGTCGGAATAGACGGTATCGAACGCCTGAATACCCGCAGAGCGCGCGGCCTGCAAAATGGAACAGCGTGCGAAcagc < 1:559055/239‑1 (MQ=255) cgcgGCGGGCGTGATCCACTTCTTTCT‑GGGTCGGTGCGTAGAGGTTGTGCAGCAGATCAATCTGACGCGGGTTGATCAGCGATTTGCCGTCAAAGCCCAGCTGTTTGATGTGGGCGGCTTCTTGCAGAAATCCGGCTTCGTTGTTAGCGTCGGAATAGACGGTATCGAACGCCTGAATACCCGCAGAGCGCGCGGCCTGCAAAATGGAACAGCGTGCGAACAGCAGTTCAGttccttcc < 1:547627/239‑1 (MQ=255) gcggcgGGAGTGGTCCACTTCTTGCT‑GTGGCGGTGCGTAGAGGGTGTGCCGCAGATCAATCTGACGCGGGGTGGTCAGCGATTTGCCGTCAAAGCCCAGCTGTTTGATGTGGGCGGCTTCTTGCAGAAATCCGGCTTCGTTGTTAGCGTCGGAATAGACGGTATCGAACGCCTGAATACCCGCAGAGCGCGCGGCCTGCAAAATGGAACAGCGTGCGAACAGCAGTTCAGttccttcc < 2:1078178/238‑1 (MQ=255) gggcgTGCTTCACTTCTTTCT‑GGGTCGGTGCGTAGAGGTTGTGCAGCAGATCAATCTGGCGCGGGTTGATCAGCGATTTGCCGTCAAAGCCCAGCTGTTTGATGTTGGCGGCTTCTTGCAGAAATCCGGCTTCGTTGTTAGCGTCGGAATAGACGGTATCGAACGCCTGAATACCCGCAGAGCGCGCGGCCTGTAAAATGGAACAGGGTGCGAACATCAGTTCAGTTCCTTCCGGGGa < 2:181356/238‑1 (MQ=255) gggcgTGATCCACTTCTTTCC‑GGGTCGGTGCGTAGAGGTTGTGCAGCAGAGCAATCTGACGCGGGTTGATCAGCGATTTGCCGGCAAAGCCCAGCTGTTTGATGTGGGCGGCTTCTTGCAGAAATCCGGCTTCGTTGTTAGCGTCGGAATAGACGGTATCGAACGCCTGAATACCCGCAGAGCGCGCGGCCTGCAAAATGGAACAGCGTGCGAACAGCAGTTCAGTTCCTTCCGGGGa < 2:456222/238‑1 (MQ=255) cACTTCTTTCT‑GGGTCGGTGCGTAGAGGTTGTGCAGCAGATCAATCTGACGCGGGTTGATCAGCGATTTGCCGTCAAAGCCCAGCTGTTTGATGTGGGCGGCTTCTTGCAGAAATCCGGCTTCGTTGTTAGCGTCGGAATAGACGGTATCGAACGCCTGAATACCCGCAGAGCGCGCGGCCTGCAAAATGGAACAGCGTGCGAACAGCAGTTCAGTTCCTTCCGGGGAGCGTTCTGTAc < 1:443808/239‑1 (MQ=255) cttcttTCG‑GGGTCGGTGCGTAGAGGTTGTGCAGCAGATCAATCTGACGCGGGTTGATCAGCGATTTGCCGTCAAAGCCCAGCTGTTTGATGTGGGCGGCTTCTTGCAGAAATCCGGCTTCGTTGTTAGCGTCGGAATAGACGGTATCGAACGCCTGAATACCCGCAGAGCGCGCGGCCTGCAAAATGGAACAGCGTGCGAACAGCAGTTCAGTTCCTTCCGGGGAGCGTTCTGTACGc < 2:951786/239‑1 (MQ=255) ttct‑GGGTCGGTGCGTAGAGGTTGTGCAGCAGATCAATCTGACGCGGGTTGATCAGCGATTTGCCGTCAAAGCCCAGCTGTTTGATGTGGGCGGCTTCTTGCAGAAATCCGGCTTCGTTGTTAGCGTCGGAATAGACGGTATCGAACGCCTGAATACCCGCAGAGCGCGCGGCCTGCAAAATGGAACAGCGTGCGAACAGCAGTTCAGTTCCTTCCGGGGAGCGTTCTGTACGCAGGtt < 1:308899/239‑1 (MQ=255) tct‑GTGTCGGTGCGTAGAGGTTGTGCAGCAGATCAATCTGACGCGGGTTCATCAGCGATTTCCTCTCAAAGCTCCGCTGTGTAATGTAGGCGGCTTCTTGAAGAAATCCGGCGTCGATCGTAGCGTCGGAATAGAGGGTATCGATTGACTCAATCCCCTCAGAGCTCGTAGCCTTCGAAATGGCCCAGCGTGCGAACCGCAGTTTAGTTCTATCCGGGGAGTGTTCTGGACTGAGGTTg < 2:171281/239‑1 (MQ=255) tct‑GGGTCGGTGCGTAGAGGTTGTGCAGCAGATCAATCTGACGCGGGTTGATCAGCGATTTGCCGTCAAAGCCCAGCTGTTTGATGTGGGCGGCTTCTTGCAGAAATCCGGCTTCGTTGTTAGCGTCGGAATAGACGGTATCGAACGCCTGAATACCCGCCGAGCGCGCGGCCTGCAAAATGGCACAGCGTGCGACCAGCAGTTCAGTTCCTTCCGGGGAGCGCTCTGTACGCAGGtt > 2:443808/1‑238 (MQ=255) tct‑GGGTCGGTGCGTAGAGGTTGTGCAGCAGATCAATCTGACGCGGGTTGATCAGCGATTTGCCGTCAAAGCCCAGCTGTTTGATGTGGGCGGCTTCTTGCAGAAATCCGGCTTCGTTGTTAGCGTCGGAATAGACGGTATCGAACGCCTGAATACCCGCAGAGCGCGCGGCCTGCAAAATGGAACAGCGTGCGAACAGCAGTTCAGTTCCTTCCGGGGAGCGTTCTGTACGCAGGtt > 2:121039/1‑238 (MQ=255) tct‑GGGTCGGTGCGTAGAGGTTGTGCAGCAGATCAATCTGACGCGGGTTGATCAGCGATTTGCCGTCAAAGCCCAGCTGTTTGATGTGGGCGGCTTCTTGCAGAAATCCGGCTTCGTTGTTAGCGTCGGAATAGACGGTATCGAACGCCTGAATACCCGCAGAGCGCGCGGCCTGCAAAATGGAACAGCGTGCGAACAGCAGTTCAGTTCCTTCCGGGGAGCGTTCTGTACGCAGGtt > 1:1095485/1‑238 (MQ=255) tct‑GGGTCGGTGCGTAGAGGTTGTGCAGCAGATCAATCTGACGCGGGTTGATCAGCGATTTGCCGTCAAAGCCCAGCTGTTTGATGTGGGCGGCTTCTTGCAGAAATCCGGCTTCGTTGTTAGCGTCGGAATAGACGGTATCGAACGCCTGAATACCCGCAGAGCGCGCGGCCTGCAAAATGGAACAGCGTGCGAACAGCAGTTCAGTTCCTTCCGGGGAGCGTTCTGTACGCAGGTTg < 1:737446/239‑1 (MQ=255) gggTCGGTGCGTAGAGGTTGTGCAGCAGATCAATCTGACGCGGGTTGATCAGCGATTTGCCGTCAAAGCCCAGCTGTTTGATGTGGGCGGCTTCTTGCAGAAATCCGGCTTCGTTGTTAGCGTCGGAATAGACGGTATCGAACGCCTGAATACCCGCAGAGCGCGCGGCCTGCAAAATGGAACAGCGTGCGAACAGCAGTTCAGTTCCTTCCGGGGAGCGTTCTGTACGCAGGTTgcgc < 1:787449/239‑1 (MQ=255) ggTCGGTGCGTAGAGGTTGTGCAGCAGATCAATCTGACGCGGGTTGATCAGCGATTTGCCGTCAAAGCCCAGCTGTTTGATGTGGGCGGCTTCTTGCAGAAATCCGGCTTCGTTGTTAGCGTCGGAATAGACGGTATCGAACGCCTGAATACCCGCAGAGCGCGCGGCCTGCAAAATGGAACAGCGTGCGAACAGCAGTTCAGTTCCTTCCGGGGAGCGTTCTGTACGCAGGTTgcgc > 1:976477/1‑238 (MQ=255) gTCGGTGCGTAGAGGTTGTGCAGCAGATCAATCTGACGCGGGTTGATCAGCGATTTGCCGTCAAAGCCCAGCTGTTTGATGTGGGCGGCTTCTTGCAGAAATCCGGCTTCGTTGTTAGCGTCGGAATAGACGGTATCGAACGCCTGAATACCCGCAGAGCGCGCGGCCTGCAAAATGGAACAGCGTGCGAACAGCAGTTCAGTTCCTTCCGGGGAGCGTTCTGTACGCAGGTTGCGcac > 1:532399/1‑239 (MQ=255) cGGTGCGTAGAGGTTGTGGAGCAGATCAATCTGACGCGGGTTGATCAGCGATTTGCCGTCAAAGCCCAGCTGTTTGATGTGGGCGGCTTCTTGCAGAAATCCGGCTTCGTTGTTAGCGTCGGAATAGACGGTATCGAACGCCTGAATACCCGCAGAGCGCGCGGCCTGCAAAATGGAACAGCGTGCGAACAGCAGTTCAGTTCCTTCCGGGGAGCGTTCTGTACGCAGGTTGCGcaca < 2:876125/238‑1 (MQ=255) cGGTGCGTAGAGGTTGTGCAGCAGATCAATCTGACGCGGGTTGATCAGCGATTTGCCGTCAAAGCCCAGCTGTTTGATGTGGGCGGCTTCTTGCAGAAATCCGGCTTCGTTGTTAGCGTCGGAATAGACGGTATCGAACGCCTGAATACCCGCAGAGCGCGCGGCCTGCAAAATGGAACAGCGTGCGAACAGCAGTTCAGTTCCTTCCGGGGAGCGTTCTGTACGCAGGTTGCGcaca < 1:121039/238‑1 (MQ=255) cGGTGCGTAGAGGTTGTGCAGCAGATCAATCTGACGCGGGTTGATCAGCGATTTGCCGTCAAAGCCCAGCTGTTTGATGTGGGCGGCTTCTTGCAGAAATCCGGCTTCGTTGTTAGCGTCGGAATAGACGGTATCGAACGCCTGAATACCCGCAGAGCGCGCGGCCTGCAAAATGGAACAGCGTGCGAACAGCAGTTCAGTTCCTTCCGGGGAGCGTTCTGTACGCAGGTTGCGcaca < 1:659295/238‑1 (MQ=255) cGGTGCGTAGAGGTTGTGCAGCAGATCAATCTGACGCGGGTTGATCAGCGATTTGCCGTCAAAGCCCAGCTGTTTGATGTGGGCGGCTTCTTGCAGAAATCCGGCTTCGTTGTTAGCGTCGGAATAGACGGTATCGAACGCCTGAATACCCGCAGAGCGCGCGGCCTGCAAAATGGAACAGCGTGCGAACAGCAGTTCAGTTCCTTCCGGGGAGCGTTCTGTACGCAGGTTGCGcaca < 2:532399/238‑1 (MQ=255) ggTGCGTAGAGGTTGTGCAGCAGATCAATCTGACGCGGGTTGATCAGCGATTTGCCGTCAAAGCCCAGCTGTTTGATGTGGGCGGCTTCTTGCAGAAATCCGGCTTCGTTGTTAGCGTCGGAATAGACGGTATCGACCGCCTGAATACACGCAGATCGCGCGGCCTGCAAAATGGAGCATCGTGCGAACAGCAGTTCAGTTCCTTCCGGGGAGCGTTCTGTACGCAGGTTGCGcacaca < 2:81322/239‑3 (MQ=255) ggTGCGTAGAGGTTGTGCAGCAGATCAATCTGACGCGGGTTGAGCAGCGATTTGCCGTCAAAGCCCAGCTGTTTGATGTGGGCGGCTTCTTGCAGAAATCCGGCTTCGTTGTTAGCGTCGGAATAGACGGTATCGAACGCCTGAATACCCGCAGAGCGCGCGGCCTGCAAAATGGAACAGCGTGCGAACAGCAGTTCAGTTCCTTCCGGGGAGCGTTCTGTACGCAGGTTGCGCACATa < 1:413068/239‑1 (MQ=255) ggTGAGTATATGTTGTGCAGCAAATCAGTGTGTAGGGGGTTGATCACCGATTTGCCGTCAAAGCCCAGCTGTTTGAGGTGGGCTGCTTCTTGCAAAAATCCGGCTTCGTTGTTAGCGTCGGAATAGACGGTATCGAACGCCTGAATACCCGCAGAGCGCGCGGCCTGCAAAATGGAACAGCGTGCGAACAGCAGTTCAGTTCCTTCCTGGGAGCGTTCTGTACGCAGGTTGCGCACATa < 2:670833/239‑1 (MQ=255) ttGTGCAGCAGATCAATCTGACGCGGGTTGATCAGCGATTTGCCGTCAAAGCCCAGCTGTTTGATGTGGGCGGCTTCTTGCAGAAATCCGGCTTCGTTGTTAGCGTCGGAATAGACGGTATCGAACGCCTGAATACCCGCAGAGCGCGCGGCCTGCAAAATGGAACAGCGTGCGAACAGCAGTTCAGTTCCTTCCGGGGAGCGTTCTGTACGCAGGTTGCGCACATa > 2:773974/1‑227 (MQ=255) ttGTGCAGCAGATCAATCTGACGCGGGTTGATCAGCGATTTGCCGTCAAAGCCCAGCTGTTTGATGTGGGCGGCTTCTTGCAGAAATCCGGCTTCGTTGTTAGCGTCGGAATAGACGGTATCGAACGCCTGAATACCCGCAGAGCGCGCGGCCTGCAAAATGGAACAGCGTGCGAACAGCAGTTCAGTTCCTTCCGGGGAGCGTTCTGTACGCAGGTTGCGCACATa < 1:773974/227‑1 (MQ=255) ttGTGCAGCAGATCAATCTGACGCGGGTTGATCAGCGATTTGCCGTCAAAGCCCAGCTGTTTGATGTGGGCGGCTTCTTGCAGAAATCCGGCTTCGTTGTTAGCGTCGGAATAGACGGTATCGAACGCCTGAATACCCGCAGAGCGCGCGGCCTGCAAAATGGAACAGCGTGCGAACAGCAGTTCAGTTCCTTCCGGGGAGCGTTCTGTACGCAGGTTGCGCACATAGTCTTCTGCAcc > 1:262227/1‑239 (MQ=255) | CTACCCGTTCTTGTCGTTGAGATTGTTCAATTTTCTGCGTCATTTTGATTGCCTTATTCTTCGCGGATGCCGGAAAGTTCTGCACGGGAGAGCACCAGACGGGCGCGATCGATAACCGGACCGTCCACCATCTTGCCGTTCAGGGAAACCACGCCGAGGCCTTCGCGAGCGGCGGCTTCAGCGGCTTCTACGACGCGGCGGGCGTGATCCACTTCTTTCT‑GGGTCGGTGCGTAGAGGTTGTGCAGCAGATCAATCTGACGCGGGTTGATCAGCGATTTGCCGTCAAAGCCCAGCTGTTTGATGTGGGCGGCTTCTTGCAGAAATCCGGCTTCGTTGTTAGCGTCGGAATAGACGGTATCGAACGCCTGAATACCCGCAGAGCGCGCGGCCTGCAAAATGGAACAGCGTGCGAACAGCAGTTCAGTTCCTTCCGGGGAGCGTTCTGTACGCAGGTTGCGCACATAGTCTTCTGCACC > NC_000913/649529‑650004 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |