Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,173,361 Δ2 bp 100% pseudogene (1‑2/442 nt) gatC ← pseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,173,3610C.83.1% 233.4 / 33.5 71pseudogene (2/442 nt)gatCpseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC
Reads supporting (aligned to +/- strand):  ref base C (1/11);  new base . (39/20);  total (40/31)
Fisher's exact test for biased strand distribution p-value = 2.76e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: Variant not supported by required number of reads on each strand.
Rejected as polymorphism: Polymorphic indel expands or contracts a homopolymer stretch.
*NC_0009132,173,3620C.83.1% 229.8 / 27.7 71pseudogene (1/442 nt)gatCpseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC
Reads supporting (aligned to +/- strand):  ref base C (0/10);  new base . (39/20);  total (40/31)
Fisher's exact test for biased strand distribution p-value = 8.84e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: Variant not supported by required number of reads on each strand.
Rejected as polymorphism: Polymorphic indel expands or contracts a homopolymer stretch.

GCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATG‑CACGGCGACCGCC‑ATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCAC‑ACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCACCCCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATCAGCCCCATCACA  >  NC_000913/2173123‑2173592
                                                                                                                                                                                                                                                 ||                                                                                                                                                                                                                                      
actgtAGCCGCCAGTGGGGTATGCAAACCAAGATTTTGCGGTGAGACCCACAGGGGGATGCTAATAATGACGACACCCGAGATTAAGGTGCGGAACGAATTGCCACGAGG‑CAGGGCGACCGCC‑ATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCAC‑ACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTGGCCGATaccac                                                                                                                                                                                                                                         <  2:227906/235‑1 (MQ=255)
      gccgccAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATG‑CACGGCGACCGCC‑ATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCAC‑ACCAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACC‑CAccgccg                                                                                                                                                                                                                                  >  1:677605/1‑239 (MQ=255)
           cAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATG‑CACGGCGACCGCC‑ATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCAC‑ACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGt                                                                                                                                                                                                                             >  1:458919/1‑238 (MQ=255)
                   tgtgCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATG‑CACGGCGACCGCC‑ATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCAC‑ACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCcagcag                                                                                                                                                                                                                     <  1:1203670/238‑1 (MQ=255)
                     tgCAAACCAATAGTTTGCGTCGCGATCCACAGGGGGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATG‑CACGGCGACCGCC‑ATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCAC‑ACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAgcagca                                                                                                                                                                                                                   >  1:428234/1‑238 (MQ=255)
                        aaaCAAATAGTTGGCGTGCGGATCCACAGGGTGAGCCTCATATGGAGGCCACCGGAGATTAAGGGGCGAAACAGATTCCCACAATG‑CACGGCGACCGCC‑ATCGCCACGAAGAAGCCGAGGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCAC‑ACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGc                                                                                                                                                                                                               <  2:412700/239‑1 (MQ=255)
                          aCCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATG‑CACGGCGACCGCC‑ATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCAC‑ACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCg                                                                                                                                                                                                             >  1:924895/1‑239 (MQ=255)
                             aaTAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATG‑CACGGCGACCGCC‑ATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCAC‑ACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGa                                                                                                                                                                                                           >  2:240369/1‑238 (MQ=255)
                                   ttGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATG‑CACGGCGACCGCC‑ATCGCCACGAAGAAGCGGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCAC‑ACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGc                                                                                                                                                                                                    <  1:1135139/239‑1 (MQ=255)
                                      cGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATG‑CACGGCGACCGCC‑ATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCAC‑ACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCaa                                                                                                                                                                                                 >  1:2316/1‑239 (MQ=255)
                                            gATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATG‑CACGGCGACCGCC‑ATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCAC‑ACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAgg                                                                                                                                                                                            <  1:240369/238‑1 (MQ=255)
                                                          tGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATG‑CACGGCGACCGCC‑ATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCAC‑ACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGAccgccg                                                                                                                                                                             >  1:1163450/1‑239 (MQ=255)
                                                          tGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATG‑CACGGCGACCGCC‑ATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCAC‑ACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGAccgccg                                                                                                                                                                             >  1:755577/1‑239 (MQ=255)
                                                          tGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATG‑CACGGCGACCGCC‑ATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCAC‑ACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGAccgccg                                                                                                                                                                             >  1:794834/1‑239 (MQ=255)
                                                          tGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATG‑CACGGCGACCGCC‑ATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCAC‑ACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGAccgccg                                                                                                                                                                             >  1:799271/1‑239 (MQ=255)
                                                               atagttatgaCCCCCGAGATAAGGGGGCGGAACAGATTCCCCCGATG‑CACGGCGACCGCC‑ATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCAC‑ACAAACAGCAATAAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCGAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACtt                                                                                                                                                                        <  1:1130324/233‑1 (MQ=255)
                                                               aaaaTGAGCACACCCGAGATTAAGGTCGGGAACAAATTCCCCCTAGG‑CCGGCCGCCCGCC‑ATCGCCACGAAGAAGCCGATGGTGGCAAGATCCCCAAACGGCAGCACCGGATTACCCGGCAC‑ACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACtt                                                                                                                                                                        <  2:986281/237‑1 (MQ=255)
                                                               aTAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATG‑CACGGCGACCGCC‑ATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCAC‑ACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGc                                                                                                                                                                                                               >  2:541307/1‑200 (MQ=255)
                                                               aTAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATG‑CACGGCGACCGCC‑ATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCAC‑ACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGc                                                                                                                                                                                                               <  1:541307/200‑1 (MQ=255)
                                                               aTAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATG‑CACGGCGACCGCC‑ATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCAC‑ACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACt                                                                                                                                                                         >  2:338255/1‑238 (MQ=255)
                                                               aTAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATG‑CACGGCGACCGCC‑ATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCAC‑ACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACt                                                                                                                                                                         >  2:436507/1‑238 (MQ=255)
                                                               aTAAGGAGGACCCCCGAGATTAAGGGGGGGAACGTATTCCCACGAGG‑CACGGCGCCCGCC‑ACCGCCACGAAGAAGCCGAGGGTGGAAAGATCGCCAAACGGCAGCACCTGATTACCCGGCAC‑ACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATAC‑‑CCCCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACtt                                                                                                                                                                        <  2:159604/239‑1 (MQ=255)
                                                                            ccGAGATTAAGGTGCGGAACAGATTTCCACGATG‑CACGGCGACCGCC‑ATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCAC‑ACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACg                                                                                                                                                            >  1:291976/1‑238 (MQ=255)
                                                                               agaTTAAGGTGCGGAACAGATTTCCACGATG‑CACGGCGACCGCC‑ATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCAC‑ACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACt                                                                                                                                                         >  1:848385/1‑238 (MQ=255)
                                                                                    agggagcagAACAGTTTGCCACTAGG‑CACGGCGACCGCA‑ACCGCCACGAAGAGGCGAATGGTGGCAAGACCCCCAAACGGCAGCACCTGATTCCCCGGCAC‑ACAAACAGCAATTAAAATGGTGGGTGGAAAAAAAATCAGCCTTGCCGATAC‑‑CCCCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGc                                                                                                                                                   <  2:1094584/231‑1 (MQ=255)
                                                                                    agggagCGGAAGAGATCACCACGAGT‑CGCGGCACCCACC‑GTCGCCAAAAAGAAGCCGAGGGTGACAAGATCGACAAACGGCAGCACCTGATACCCCGGCAC‑ACAACCAGCATATAAAAGGGTGATTGGGAGAAAAACCAGCGTTGCCATTAC‑‑CCCCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGc                                                                                                                                                   <  2:632206/234‑1 (MQ=255)
                                                                                    agggTGCGGAACAGATTCCCGCGCTG‑CCCGCGGCCCCCC‑GACCCCACGAAGAACCCGATGGTGCAAAGATCGCCAACGGGCAGCCCTTAATTCCCCGGCAC‑CCAAACAGAAATAAAATTGGTGAGTGGGAAAAAAATCA‑GCTTGGCG‑TATACCCCCGCCGTATGTCCCAGCAGCACCGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGc                                                                                                                                                   <  2:848385/237‑1 (MQ=255)
                                                                                    agggTGCAGAACAGATTTCCACGATG‑CACGGCGACCGCC‑ATCGCCACGAAGAAGCCGAGGGTGGCAAGATCCCCAAACGGCAGCACCTGATTCCCCGGCAC‑ACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGc                                                                                                                                                   <  1:935236/237‑1 (MQ=255)
                                                                                    aaGGGGCGGAACAAATTCCCACGATG‑CACGGCGACCGCC‑ATCGCCACGAAGAAGCCGATGGTGGCAAGATGGCCAAACGGCAGCACCTGATTACCCGGCAC‑ACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGc                                                                                                                                                   <  1:983899/239‑1 (MQ=255)
                                                                                     gggTGGGGACAAGATTCCCCCGATG‑C‑CGGCGCCCCCCGATCGCCACAAAGAGACGAATGGTGGCAAGTTCCCCAAAGGGCAGCCCCTGATTCCCCGCCCC‑ACAAACAGCATTTAAAATGGGGAGGGGGATAAAAACAAGGCTTGCCGAT‑CC‑CTCCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGc                                                                                                                                                   <  2:1002915/237‑1 (MQ=255)
                                                                                     aGGTGGGAAACAGATTACCACGTGC‑CCCGCCGCCCGCC‑ACCGCAAGAAAAAGGCCAAGGGGGGCAAGACCCCCAACGGGCAGCCCGTGATACCCGGGCAC‑AAAACCAGCATTAAAAATGGTGAGTGGAAAAAAAACCAGGCTGGCCGAT‑CC‑CCCCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGc                                                                                                                                                   <  2:929814/238‑1 (MQ=255)
                                                                                     aGGTGCAGAACAGGTTTCCCCGGTGACAGGGGGCCGGCC‑ACCCCCAGAAAAAACCCAAGGGTGGCAAGATCGCAAACCGGCGGCACCTGTTTCCCCGGCAC‑ACAAACAGAAATTAAAAGGGAGAGTGGAAAAAAAATCGGGCTTGCCGATACCA‑‑CCGCCGTGTGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGc                                                                                                                                                   <  2:630727/239‑1 (MQ=255)
                                                                                          cGGAACAGATTTCCACGATG‑CACGGCGACCGCC‑ATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCAC‑ACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCtt                                                                                                                                             >  1:210452/1‑239 (MQ=255)
                                                                                          cGGAACAGATTTCCACGATG‑CACGGCGACCGCC‑ATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCAC‑ACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCtt                                                                                                                                             >  2:753390/1‑239 (MQ=255)
                                                                                                  aTTTCCACGATG‑CACGGCGACCGCC‑ATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCAC‑ACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGTCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATgt                                                                                                                                     >  1:732199/1‑238 (MQ=255)
                                                                                                  aTTTCCACGATG‑CACGGCGACCGCC‑ATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCAC‑ACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGCACTCCTGACCGCCGAACTTCGCCTGTAATCGACTACGAATTTGCTCAGCGATgg                                                                                                                                     >  2:1214017/1‑239 (MQ=255)
                                                                                                   tttCCCCGATG‑CACGGCGACCGCC‑ATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACTTGATTCCCCGGCAC‑ACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGAt                                                                                                                                       <  1:1034431/236‑1 (MQ=255)
                                                                                                       cACGATG‑CACGGCGACCGCC‑ATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCAC‑ACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGtt                                                                                                                                >  1:609855/1‑239 (MQ=255)
                                                                                                          gATC‑CACGGCGACCGCC‑ATCGCCACGAAGAAGCCGATGGTGGCAAGACCGCCAAACGGCAACACCTGATTCCCCGGCAC‑AAAAACAGAAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATCCCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTaaa                                                                                                                             <  1:1130920/239‑1 (MQ=255)
                                                                                                           gtgcaccGGCCACCCCC‑ACCGCCACGAAGAAGCCGATGGTGCAAAAATCGCAAAGCGGCAGCCCCGGATTACCCGGCAC‑ACAAACAGAAATAAAAATGGTGAGTGGAATAAAAACCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTaaa                                                                                                                             <  2:210452/233‑1 (MQ=255)
                                                                                                            tG‑CACGGCGACCGCC‑ATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCAC‑ACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGAt                                                                                                                                       <  1:815198/227‑1 (MQ=255)
                                                                                                            tG‑CACGGCGACCGCC‑ATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCAC‑ACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGAt                                                                                                                                       >  2:815198/1‑227 (MQ=255)
                                                                                                            tG‑CACGGCGACCGCC‑ATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCAC‑ACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAAcc                                                                                                                           >  2:467608/1‑239 (MQ=255)
                                                                                                            gg‑CACGGCGACCGCC‑ATCGCCACGAAGAAGCCGATGGTGGCAAGACCGCCAAACGGCAGCACCTGATTACCCGGCAC‑ACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAAc                                                                                                                            <  1:924565/237‑1 (MQ=255)
                                                                                                                     gcccGCC‑ATCGCCACGAAGAAGCCGATGGTGGCAAGACCGCCAAACGGCAGCACCTGATTACCCGGCAC‑ACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCatga                                                                                                                   <  1:385925/237‑1 (MQ=255)
                                                                                                                       ccGCC‑ATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCAC‑ACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCatgatg                                                                                                                 >  1:460634/1‑239 (MQ=255)
                                                                                                                                gcaactaagaagCTGTTGCGGGCAATCTCGCCAAGCGGGTGCCCATGA‑TACCCGCCCCAACAAACAGCAATAAAAATGGGGACGGGCATAAAAACCAGTCTCCCCATTACCA‑‑CCGCCATATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGCCTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAAt                                                                                                         <  2:1108650/233‑1 (MQ=255)
                                                                                                                                gCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCGAATTACCCGGCAC‑ACAAACAGCAATAAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAAt                                                                                                         <  1:32368/239‑1 (MQ=255)
                                                                                                                                            ccGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCAC‑ACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCAt                                                                                             >  1:1251370/1‑239 (MQ=255)
                                                                                                                                            ccGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCAC‑ACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGAAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCAt                                                                                             >  2:441839/1‑239 (MQ=255)
                                                                                                                                                tggtggCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCAC‑ACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTagc                                                                                         >  2:791102/1‑239 (MQ=255)
                                                                                                                                                       aaGATCGCCAAACGGCAGCACCTGATTACCCGGCAC‑ACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATc                                                                                   >  1:650601/1‑238 (MQ=255)
                                                                                                                                                                           ccTGATTACCCGGCGC‑ACAAACAGCAATAAAAATGGTGAGTGGAATAAAAATCAGGCTTGCCGATAC‑‑CCCCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGcc                                                              <  1:354823/239‑1 (MQ=255)
                                                                                                                                                                             tGATTACCGGGCAC‑ACAAACAGCAATAAAAAGTGTGAGTGGGATAAAAATAAGGCTTGCCGATAC‑‑CCCCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAg                                                            <  1:985986/239‑1 (MQ=255)
                                                                                                                                                                              gATTACCCGGCAC‑ACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCa                                                             >  1:229787/1‑237 (MQ=255)
                                                                                                                                                                                   cccGGCAC‑ACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCaa                                                      >  1:1041039/1‑239 (MQ=255)
                                                                                                                                                                                      ggCAC‑ACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAAccat                                                                                                                         >  2:1093749/1‑169 (MQ=255)
                                                                                                                                                                                      ggCAC‑ACAAACAGCAATAAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAAccat                                                                                                                         <  1:1093749/169‑1 (MQ=255)
                                                                                                                                                                                        cac‑acaAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGCCCGCCGAACTTCGCCTGTAAACGCCTACGAGCCTGCTTAGCGATGGCCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCCGCATCACTGCCGCCGTTTTTACCGCCACCTGCAATacc                                                  >  2:1023117/1‑237 (MQ=255)
                                                                                                                                                                                         ac‑acaAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATaca                                                  >  2:220094/1‑237 (MQ=255)
                                                                                                                                                                                              aaaCAGAAATTAAAAGGGTGAGGGGGAAAAAAATCAGCCTGGCCGATCCCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCGGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTg                                            <  2:889988/239‑1 (MQ=255)
                                                                                                                                                                                                      aTTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTa                                    >  1:889988/1‑239 (MQ=255)
                                                                                                                                                                                                            aTGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCcgc                              >  1:355254/1‑239 (MQ=255)
                                                                                                                                                                                                                  cgTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCACTACACCTTTGACATCGTAACCCGCGAGGAt                        >  1:557516/2‑239 (MQ=255)
                                                                                                                                                                                                                  aGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGTCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCATGTAAACGACTACGAGCCTGCTTAACGATGGCCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATAACGGCCGCCGTTTTTCTCGCCAGCTGCACTAAACCTTTGACATCGGAACCCCCGAGGAt                        >  2:320134/1‑239 (MQ=255)
                                                                                                                                                                                                                  aGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATc                                                                                                        >  1:392308/1‑159 (MQ=255)
                                                                                                                                                                                                                  aGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGAt                        >  1:730282/1‑239 (MQ=255)
                                                                                                                                                                                                                      agATAAAAATCAGGCGTGCCGATAC‑‑CCCCGCCGTATGCCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGcc                     <  1:513760/237‑1 (MQ=255)
                                                                                                                                                                                                                       gaaaaaaaCCAGGCTTGCCGAT‑CC‑CCCCGCCGTATGTCCCAGAAGAACCGCGGGACCAAGGCCAATCAGGAACTCCTGCCCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCg                    <  2:609855/235‑1 (MQ=255)
                                                                                                                                                                                                                        aaaaaaaaCAGGTTTTCCGA‑ACCCCCCCGCCGTATGTCCCAGCAGCACCGCGGGATCAGGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCg                    <  2:652193/236‑1 (MQ=255)
                                                                                                                                                                                                                                  ggCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATCAGcccc        >  2:470843/1‑239 (MQ=255)
                                                                                                                                                                                                                                       gCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATCAGCCCCATcac   >  1:347450/1‑239 (MQ=255)
                                                                                                                                                                                                                                          cagctacccccGCCTTTTGTCCCAGCAGCACGGCCGGATCAAGGCCAATCAGGAACCCCTGACCGCCAAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATCAGCCCCATcaca  <  1:1214017/232‑1 (MQ=255)
                                                                                                                                                                                                                                                 ||                                                                                                                                                                                                                                      
GCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATG‑CACGGCGACCGCC‑ATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCAC‑ACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCACCCCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATCAGCCCCATCACA  >  NC_000913/2173123‑2173592

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑