New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	= 4345636	89 (0.790)	4 (0.040)	4/420	NT	4.4%	intergenic (‑78/+44)	yjdF/fumB	DUF2238 family inner membrane protein/anaerobic class I fumarate hydratase (fumarase B)
	?	NC_000913	= 4345643	88 (0.800)					intergenic (‑85/+37)	yjdF/fumB	DUF2238 family inner membrane protein/anaerobic class I fumarate hydratase (fumarase B)
Rejected: Frequency below/above cutoff threshold.

TAACGTCTGGCGGTGGCAAGCAGTAGCTGCACGACAATAATCACCGGTGTCACTTCCATCAGCCAGGTGAGTTTGTCATGGGCCGAAATGCCGGTATAAATCAGGATTAACGTTAGCGTCAGTGCGCTGGTGTTAAGAATTAACGGCTTGAGTGTGCGGGTCATGGTGAGTTCGCAAATCAGGGAAAATACTGACTATTCACCAGCAAGCGTGAAATTACAATCGCAAAAGAGGTGGCCAGG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/4345395‑4345636 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑gccaggTGATCCCCCTGGCCACCTCTTTTGCGATTGTAATTTCACGCTTGCTGGTGAATAGTCAGTATTTTCCCTGATTTGCGAACTCACCATGACCCGCACACTCAAGCCGTTAATTCTTAACACCAGCGCACTGACGCTAACGTTAATCCTGATTTATACCGGCATTTCGGCCCATGACAAACTCACCTGGCTGATGGAAGTGACACCGGTGATTATTGTCGTGCAGCTACTGCTTGCCA  <  NC_000913/4345643‑4345408                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                TAACGTCTGGCGGTGGCAAGCAGTAGCTGCACGACAATAATCACCGGTGTCACTTCCATCAGCCAGGTGAGTTTGTCATGGGCCGAAATGCCGGTATAAATCAGGATTAACGTTAGCGTCAGTGCGCTGGTGTTAAGAATTAACGGCTTGAGTGTGCGGGTCATGGTGAGTTCGCAAATCAGGGAAAATACTGACTATTCACCAGCAAGCGTGAAATTACAATCGCAAAAGAGGTGGCC                                                                                                                                                                                                                                                 <  1:1236013/239‑1                                                                                                                                                                                     AGGGAAAATACTGACTATTCACCAGCAAGCGTGAAATTATAATCGCAAAAGAGGTGGCCAGGTGATCCCCCTGGCCACCTCTTTTGCGATTGTAATTTCACGCTTGCTGGTGAATAGTCAGTATTTTCCCTGATTTGCGAACTCACCATGACCCGCACACTCAAGCCGTTAATTCTTAACACCAGCGCACTGACGCT                                                                                                       <  1:340668/197‑1                                                                                                                                                                                     AGGGAAAATACTGACTATTCACCAGCAAGCGTGAAATTATAATCGCAAAAGAGGTGGCCAGGTGATCCCCCTGGCCACCTCTTTTGCGATTGTAATTTCACGCTTGCTGGTGAATAGTCAGTATTTTCCCTGATTTGCGAACTCACCATGACCCGCACACTCAAGCCGTTAATTCTTAACACCAGCGCACTGACGCT                                                                                                       >  2:340668/1‑197                                                                                                                                                                                                      TTCACCAGCAAGCGTGAAATTACAATCGCAAAAGAGGTGGCCAGGTGATCCCCCTGGCCACCTCTTTTGCGATTGTAATTTCACGCTTGCTGGTGAATAGTCAGTATTTTCCCTGATTTGCGAACTCACCATGACCCGCACACTCAAGCCGTTAATTCTTAACACCAGCGCACTGACGCTAACGTTAATCCTGATTTATACCGGCATTTCGGCCCATGACAAACTCACC                                                      >  1:480876/1‑229                                                                                                                                                                                                      TTCACCAGCAAGCGTGAAATTACAATCGCAAAAGAGGTGGCCAGGTGATCCCCCTGGCCACCTCTTTTGCGATTGTAATTTCACGCTTGCTGGTGAATAGTCAGTATTTTCCCTGATTTGCGAACTCACCATGACCCGCACACTCAAGCCGTTAATTCTTAACACCAGCGCACTGACGCTAACGTTAATCCTGATTTATACCGGCATTTCGGCCCATGACAAACTCACC                                                      <  2:480876/229‑1                                                                                                                                                                                                                                             GCCAGGTGATCCCCCTGGCCACCTCTTTTGCGATTGTAATTTCACGCTTGCTGGTGAATAGTCAGTATTTTCCCTGATTTGCGAACTCACCATGACCCGCACACTCAAGCCGTTAATTCTTAACACCAGCGCACTGACGCTAACGTTAATCCTGATTTATACCGGCATTTCGGCCCATGACAAACTCACCTGGCTGATGGAAGTGACACCGGTGATTATTGTCGTGCAGCTACTGCTT      >  2:663591/1‑238                                                                                                                                                                                                                                             GCCAGGTGATCCCCCTGGCCACCTCTTTTGCGATTGTAATTTCACGCTTGCTGGTGAATAGTCAGTATTTTCCCTGATTTGCGAACTCACCATGACCCGCACACTCAAGCCGTTAATTCTTAACACCAGCGCACTGACGCTAACGTTAATCCTGATTTATACCGGCATTTCGGCCCATGACAAACTCACCTGGCTGATGGAAGTGACACCGGTGATTATTGTCGTGCAGCTACTGCTT      >  2:953332/1‑238                                                                                                                                                                                                                                             GCCAGGTGATCCCCCTGGCCACCTCTTTTGCGATTGTAATTTCACGCTTGCTGGTGAATAGTCAGTATTTTCCCTGATTTGCGAACTCACCATGACCCGCACACTCAAGCCGTTAATTCTTAACACCAGCGCACTGACGCTAACGTTAATC                                                                                             >  1:949013/1‑151                                                                                                                                                                                                                                             GCCAGGTGATCCCCCTGGCCACCTCTTTTGCGATTGTAATTTCACGCTTGCTGGTGAATAGTCAGTATTTTCCCTGATTTGCGAACTCACCATGACCCGCACACTCAAGCCGTTAATTCTTAACACCAGCGCACTGACGCTAACGTTAATC                                                                                             <  2:949013/151‑1                                                                                                                                                                                                                                               CAGGTGATCCCCCTGGCCACCTCTTTTGCGATTGTAATTTCACGCTTGCTGGTGAATAGTCAGTATTTTCCCTGATTTGCGAACTCACCATGACCCGCACACTCAAGCCGTTAATTCTTAACACCAGCGCACTGACGCTAACGTTAATCCTGATTTATACCGGCATTTCGGCCCATGACAAACTCACCTGGCTGATGGAAGTGACACCGGTGATTATTGTCGTGCAGCTACTGCTTGCC   <  2:213035/239‑1                                                                                                                                                                                                                                                 GGTGATCCCCCTGGCCACCTCTTTTGCGATTGTAATTTCACGCTTGCTGGTGAATAGTCAGTATTTTCCCTGATTTGCGAACTCACCATGACCCGCACACTCAAGCCGTTAATTCTTAACACCAGCGCACTGACGCTAACGTTAATCCTGATTTATACCGGCATTTCGGCCCATGACAAACTCACCTGGCTGATGGAAGTGACACCGGTGATTATTGTCGTGCAGCTACTGCTTGCCA  >  2:98092/1‑238                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                TAACGTCTGGCGGTGGCAAGCAGTAGCTGCACGACAATAATCACCGGTGTCACTTCCATCAGCCAGGTGAGTTTGTCATGGGCCGAAATGCCGGTATAAATCAGGATTAACGTTAGCGTCAGTGCGCTGGTGTTAAGAATTAACGGCTTGAGTGTGCGGGTCATGGTGAGTTCGCAAATCAGGGAAAATACTGACTATTCACCAGCAAGCGTGAAATTACAATCGCAAAAGAGGTGGCCAGG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/4345395‑4345636 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑gccaggTGATCCCCCTGGCCACCTCTTTTGCGATTGTAATTTCACGCTTGCTGGTGAATAGTCAGTATTTTCCCTGATTTGCGAACTCACCATGACCCGCACACTCAAGCCGTTAATTCTTAACACCAGCGCACTGACGCTAACGTTAATCCTGATTTATACCGGCATTTCGGCCCATGACAAACTCACCTGGCTGATGGAAGTGACACCGGTGATTATTGTCGTGCAGCTACTGCTTGCCA  <  NC_000913/4345643‑4345408

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.