New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 434563689 (0.790)4 (0.040) 4/420 NT 4.4% intergenic (‑78/+44) yjdF/fumB DUF2238 family inner membrane protein/anaerobic class I fumarate hydratase (fumarase B)
?NC_000913 = 4345643 88 (0.800)intergenic (‑85/+37) yjdF/fumB DUF2238 family inner membrane protein/anaerobic class I fumarate hydratase (fumarase B)
Rejected: Frequency below/above cutoff threshold.

TAACGTCTGGCGGTGGCAAGCAGTAGCTGCACGACAATAATCACCGGTGTCACTTCCATCAGCCAGGTGAGTTTGTCATGGGCCGAAATGCCGGTATAAATCAGGATTAACGTTAGCGTCAGTGCGCTGGTGTTAAGAATTAACGGCTTGAGTGTGCGGGTCATGGTGAGTTCGCAAATCAGGGAAAATACTGACTATTCACCAGCAAGCGTGAAATTACAATCGCAAAAGAGGTGGCCAGG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/4345395‑4345636
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑gccaggTGATCCCCCTGGCCACCTCTTTTGCGATTGTAATTTCACGCTTGCTGGTGAATAGTCAGTATTTTCCCTGATTTGCGAACTCACCATGACCCGCACACTCAAGCCGTTAATTCTTAACACCAGCGCACTGACGCTAACGTTAATCCTGATTTATACCGGCATTTCGGCCCATGACAAACTCACCTGGCTGATGGAAGTGACACCGGTGATTATTGTCGTGCAGCTACTGCTTGCCA  <  NC_000913/4345643‑4345408
                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
TAACGTCTGGCGGTGGCAAGCAGTAGCTGCACGACAATAATCACCGGTGTCACTTCCATCAGCCAGGTGAGTTTGTCATGGGCCGAAATGCCGGTATAAATCAGGATTAACGTTAGCGTCAGTGCGCTGGTGTTAAGAATTAACGGCTTGAGTGTGCGGGTCATGGTGAGTTCGCAAATCAGGGAAAATACTGACTATTCACCAGCAAGCGTGAAATTACAATCGCAAAAGAGGTGGCC                                                                                                                                                                                                                                                 <  1:1236013/239‑1
                                                                                                                                                                                    AGGGAAAATACTGACTATTCACCAGCAAGCGTGAAATTATAATCGCAAAAGAGGTGGCCAGGTGATCCCCCTGGCCACCTCTTTTGCGATTGTAATTTCACGCTTGCTGGTGAATAGTCAGTATTTTCCCTGATTTGCGAACTCACCATGACCCGCACACTCAAGCCGTTAATTCTTAACACCAGCGCACTGACGCT                                                                                                       <  1:340668/197‑1
                                                                                                                                                                                    AGGGAAAATACTGACTATTCACCAGCAAGCGTGAAATTATAATCGCAAAAGAGGTGGCCAGGTGATCCCCCTGGCCACCTCTTTTGCGATTGTAATTTCACGCTTGCTGGTGAATAGTCAGTATTTTCCCTGATTTGCGAACTCACCATGACCCGCACACTCAAGCCGTTAATTCTTAACACCAGCGCACTGACGCT                                                                                                       >  2:340668/1‑197
                                                                                                                                                                                                     TTCACCAGCAAGCGTGAAATTACAATCGCAAAAGAGGTGGCCAGGTGATCCCCCTGGCCACCTCTTTTGCGATTGTAATTTCACGCTTGCTGGTGAATAGTCAGTATTTTCCCTGATTTGCGAACTCACCATGACCCGCACACTCAAGCCGTTAATTCTTAACACCAGCGCACTGACGCTAACGTTAATCCTGATTTATACCGGCATTTCGGCCCATGACAAACTCACC                                                      >  1:480876/1‑229
                                                                                                                                                                                                     TTCACCAGCAAGCGTGAAATTACAATCGCAAAAGAGGTGGCCAGGTGATCCCCCTGGCCACCTCTTTTGCGATTGTAATTTCACGCTTGCTGGTGAATAGTCAGTATTTTCCCTGATTTGCGAACTCACCATGACCCGCACACTCAAGCCGTTAATTCTTAACACCAGCGCACTGACGCTAACGTTAATCCTGATTTATACCGGCATTTCGGCCCATGACAAACTCACC                                                      <  2:480876/229‑1
                                                                                                                                                                                                                                            GCCAGGTGATCCCCCTGGCCACCTCTTTTGCGATTGTAATTTCACGCTTGCTGGTGAATAGTCAGTATTTTCCCTGATTTGCGAACTCACCATGACCCGCACACTCAAGCCGTTAATTCTTAACACCAGCGCACTGACGCTAACGTTAATCCTGATTTATACCGGCATTTCGGCCCATGACAAACTCACCTGGCTGATGGAAGTGACACCGGTGATTATTGTCGTGCAGCTACTGCTT      >  2:663591/1‑238
                                                                                                                                                                                                                                            GCCAGGTGATCCCCCTGGCCACCTCTTTTGCGATTGTAATTTCACGCTTGCTGGTGAATAGTCAGTATTTTCCCTGATTTGCGAACTCACCATGACCCGCACACTCAAGCCGTTAATTCTTAACACCAGCGCACTGACGCTAACGTTAATCCTGATTTATACCGGCATTTCGGCCCATGACAAACTCACCTGGCTGATGGAAGTGACACCGGTGATTATTGTCGTGCAGCTACTGCTT      >  2:953332/1‑238
                                                                                                                                                                                                                                            GCCAGGTGATCCCCCTGGCCACCTCTTTTGCGATTGTAATTTCACGCTTGCTGGTGAATAGTCAGTATTTTCCCTGATTTGCGAACTCACCATGACCCGCACACTCAAGCCGTTAATTCTTAACACCAGCGCACTGACGCTAACGTTAATC                                                                                             >  1:949013/1‑151
                                                                                                                                                                                                                                            GCCAGGTGATCCCCCTGGCCACCTCTTTTGCGATTGTAATTTCACGCTTGCTGGTGAATAGTCAGTATTTTCCCTGATTTGCGAACTCACCATGACCCGCACACTCAAGCCGTTAATTCTTAACACCAGCGCACTGACGCTAACGTTAATC                                                                                             <  2:949013/151‑1
                                                                                                                                                                                                                                              CAGGTGATCCCCCTGGCCACCTCTTTTGCGATTGTAATTTCACGCTTGCTGGTGAATAGTCAGTATTTTCCCTGATTTGCGAACTCACCATGACCCGCACACTCAAGCCGTTAATTCTTAACACCAGCGCACTGACGCTAACGTTAATCCTGATTTATACCGGCATTTCGGCCCATGACAAACTCACCTGGCTGATGGAAGTGACACCGGTGATTATTGTCGTGCAGCTACTGCTTGCC   <  2:213035/239‑1
                                                                                                                                                                                                                                                GGTGATCCCCCTGGCCACCTCTTTTGCGATTGTAATTTCACGCTTGCTGGTGAATAGTCAGTATTTTCCCTGATTTGCGAACTCACCATGACCCGCACACTCAAGCCGTTAATTCTTAACACCAGCGCACTGACGCTAACGTTAATCCTGATTTATACCGGCATTTCGGCCCATGACAAACTCACCTGGCTGATGGAAGTGACACCGGTGATTATTGTCGTGCAGCTACTGCTTGCCA  >  2:98092/1‑238
                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
TAACGTCTGGCGGTGGCAAGCAGTAGCTGCACGACAATAATCACCGGTGTCACTTCCATCAGCCAGGTGAGTTTGTCATGGGCCGAAATGCCGGTATAAATCAGGATTAACGTTAGCGTCAGTGCGCTGGTGTTAAGAATTAACGGCTTGAGTGTGCGGGTCATGGTGAGTTCGCAAATCAGGGAAAATACTGACTATTCACCAGCAAGCGTGAAATTACAATCGCAAAAGAGGTGGCCAGG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/4345395‑4345636
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑gccaggTGATCCCCCTGGCCACCTCTTTTGCGATTGTAATTTCACGCTTGCTGGTGAATAGTCAGTATTTTCCCTGATTTGCGAACTCACCATGACCCGCACACTCAAGCCGTTAATTCTTAACACCAGCGCACTGACGCTAACGTTAATCCTGATTTATACCGGCATTTCGGCCCATGACAAACTCACCTGGCTGATGGAAGTGACACCGGTGATTATTGTCGTGCAGCTACTGCTTGCCA  <  NC_000913/4345643‑4345408

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.