Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	836,592	T→G	16.1%	V81G (GTC→GGC)	ybiC →	putative dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	836,592	0	T	G	16.1%	159.9 / 2.9	63	V81G (GTC→GGC)	ybiC	putative dehydrogenase
Reads supporting (aligned to +/- strand):  ref base T (11/39);  new base G (11/2);  total (22/41)
Fisher's exact test for biased strand distribution p-value = 5.78e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGTCATCGCTTTGATGCTCAGACGCTGCACAGTTTTATTCAGGCTGTATTTCGTCAGATGGGTAGCGAGGAACAAGAAGCGAAATTAGTTGCCGATCATTTAATCGCGGCAAACCTGGCAGGGCATGATTCACATGGTATTGGCATGATCCCAAGCTATGTACGCTCCTGGAGTCAGGGGCACCTGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGCGGCGGTCACGCTCGATGGC‑GATCGCG‑CATTTGGTCAGGTCGC‑GGCACATGAAGCGATGGCGCTGGGGATTGAGAAAGCGCATCAGCACGGTATTGCCGCCGTGGCGCTACATAACTCGCATCATATCGGCCGTATCGGTTACTGGGCGGAGCAGTGTGCAGCGGCGGGGTTTGTCTCTATCCACTTTGTTAGCGTGGTCGGTATTCCAATGGTCGCGCCGTTCCACGGTCGCGACAGC  >  NC_000913/836360‑836824                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                            ggTCATCGCTTTGATGCTCAGACGCTGCACAGTTTTATTCAGGCTGTATTTCGTCAGATGGGTAGCGAGGAACAAGAAGCGAAATTAGTTGCCGATCATTTAATCGCGGCAAACCTGGCAGGGCATGATTCACATGGTATTGGCATGATCCCAAGCTATGTACGCTCCTGGAGTCAGGGGCACCTGCAAATTAACCATCATGCCAAAACCGTTAAAGCGGCGGGGGGGGCGGTCAcag                                                                                                                                                                                                                                        >  2:956125/1‑236 (MQ=255)  gTCATCGCTTTGATGCTCAGACGCTGCACAGTTTTATTCAGGCTGTATTTCGTCAGATGGGTAGCGAGGAACAAGAAGCGAAATTAGTTGCCGATCATTTAATCGCGGCAAACCTGGCAGGGCATGATTCACATGGTATTGGCATGATCCCAAGCTATGTACGCTCCTGGAGTCAGGGGCACCTGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGCGGCGGTCACGcc                                                                                                                                                                                                                                       >  1:396679/1‑237 (MQ=255)    cATCGCTTTGATGCTCAGACGCTGCACAGTTTTATTCAGGCTGTATTTCGTCAGATGGGTAGCGAGGAACAAGAAGCGAAATTAGTTGCCGATCATTTAATCGCGGCAAACCTGGCAGGGCATGATTCACATGGTATTGGCATGATCCCAAGCTATGTACGCTCCTGGAGTCAGGGGCACCTGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGCGGCGGTCACGCTCGa                                                                                                                                                                                                                                    <  1:1225639/239‑1 (MQ=255)          tttGATGCTCAGACGCTGCACAGTTTTATTCAGGCTGTATTTCGTCAGATGGGTAGCGAGGAACAAGAAGCGAAATTAGTTGCCGATCATTTAATCGCGGCAAACCTGGCAGGGCATGATTCACATGGTATTGGCATGATCCCAAGCTATGTACGCTCCTGGAGTCAGGGGCACCTGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGGGGCGGTCACGCCCGCTGGC‑Ga                                                                                                                                                                                                                             >  1:851714/1‑239 (MQ=255)                                  tttATTCAGGCTGTATTTCGTCAGATGGGTAGCGAGGAACAAGAAGCGAAATTAGTTGCCGATCATTTAATCGCGGCAAACCTGGCAGGGCATGATTCACATGGTATTGGCATGATCCCAAGCTATGTACGCTCCTGGAGTCAGGGGCACCTGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGCGGCGGTCACGCTCGATGGC‑GATCGCG‑CATTTGGTCAGGTcgc‑g                                                                                                                                                                                                     <  2:142233/237‑1 (MQ=255)                                     agtCAGTCTGTATTTCGTCAGATGGGTAGCGAGTAACAAGAAGCTAAATTAGTTGCCGATCATTTAATCGCGGCAAGCCAGGCAGGGCATGATTCACATGGTATTGGCATGATCCCAAGCTATGTACGCTCCTGGAGTCAGGGGCACCTGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGCGGCGGTCACGCGCGATGGC‑GATCGCG‑CATTTGGTCAGGTCGC‑GGcaca                                                                                                                                                                                                <  2:49950/237‑1 (MQ=255)                                     aTTCAGGCTGTATTTCGTCAGATGGGTAGCGAGGAACAAGAAGCGAAATTAGTTGCCGATCATTTAATCGCGGCAAACCTGGCAGGGCATGATTCACATGGTATTGGCATGATCCCAAGCTATGTACGCTCCTGGAGTCAGGGGCACCTGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGCGGCGGTCACGCTCGATGGC‑GATCGCG‑CATTTGGTCAGGTCGC‑GGcaca                                                                                                                                                                                                <  2:738949/239‑1 (MQ=255)                                     aTTCAGGCTGTATTTCGTCAGATGGGTAGCGAGGAACAAGAAGCGAAATTAGTTGCCGATCATTTAATCGCGGCAAACCTGGCAGGGCATGATTCACATGGTATTGGCATGATCCCAAGCTATGTACGCTCCTGGAGTCAGGGGCACCTGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGCGGCGGTCACGCTCGATGGC‑GATCGCG‑CATTTGGTCAGGTCGC‑GGcaca                                                                                                                                                                                                <  2:505696/239‑1 (MQ=255)                                      ttCAGGCTGTATTTCGTCAGATGGGTAGCGAGGAACAAGAAGCGAAATTAGTTGCCGATCATTTAATCGCGGCAAACCTGGCAGGGCATGATTCACATGGTATTGGCATGATCCCAAGCTATGTACGCTCCTGGAGTCAGGGGCACCTGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGCGGCGGTCACGCTCGATGGC‑GATCGCG‑CATTTGGTCAGGGCGC‑GGCACAt                                                                                                                                                                                               >  1:1149796/1‑239 (MQ=255)                                       tCAGGCTGTATTTCGTCAGATGGGTAGCGAGGAACAAGAAGCGAAATTAGTTGCCGATCATTTAATCGCGGCAAACCTGGCAGGGCATGATTCACATGGTATTGGCATGATCCCAAGCTATGTACGCTCCTGGAGTCAGGGGCACCTGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGCGGCGGTCACGCTCGATGGC‑GATCGCG‑CATTTGGTCAGGTCGC‑GGCACATg                                                                                                                                                                                              <  2:1149796/239‑1 (MQ=255)                                           gCTGTATTTCGTCAGATGGGTAGCGAGGAACAAGAAGCGAAATTAGTTGCCGATCATTTAATCGCGGCAAACCTGGCAGGGCATGATTCACATGGTATTGGCATGATCCCAAGCTATGTACGCTCCTGGAGTCAGGGGCACCTGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGCGGCGGTCCCCCCCGATGGC‑GATCGCG‑CATTTGGTCAGGTCGC‑GGCACATGAAGc                                                                                                                                                                                          >  1:1199808/1‑239 (MQ=255)                                           gCTGTATTTCGTCAGATGGGTAGCGAGGAACAAGAAGCGAAATTAGTTGCCGATCATTTAATCGCGGCAAACCTGGCAGGGCATGATTCACATGGTATTGGCATGATCCCAAGCTATGTACGCTCCTGGAGTCAGGGGCACCTGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGCGGCGGTCACGCTCGATGGC‑GATCGCG‑CATTTGGTCAGGTCCC‑GGCACATGAAGc                                                                                                                                                                                          >  1:1038776/1‑239 (MQ=255)                                           gCTGTATTTCGTCAGATGGGTAGCGAGGAACAAGAAGCGAAATTAGTTGCCGATCATTTAATCGCGGCAAACCTGGCAGGGCATGATTCACATGGTATTGGCATGATCCCAAGCTATGTACGCTCCTGGAGTCAGGGGCACCTGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGCGGCGGGCACGCTCACTTGC‑GATCGCG‑CTTTTGGTACAGTCGC‑GGGACATGaatc                                                                                                                                                                                          >  1:1058052/1‑237 (MQ=255)                                              gTATTTCGTCAGATGGGTAGCGAGGAACAAGAAGCGAAATTAGTTGCCGATCATTTAATCGCGGCAAACCTGGCAGGGCATGATTCACATGGTATTGGCATGATCCCAAGCTATGTACGCTCCTGGAGTCAGGGGCACCTGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGCGGCGGTCACGCTCGATGGC‑GATCGCG‑CATTTGGTCAGGTCGC‑GGCACATGAAGCGAt                                                                                                                                                                                       <  2:463047/239‑1 (MQ=255)                                               tATTTCGTCAGATGGGTAGCGAGGAACAAGAAGCGAAATTAGTTGCCGATCATTTAATCGCGGCAAACCTGGCAGGGCATGATTCACATGGTATTGGCATGATCCCAAGCTATGTACGCTCCTGGAGTCAGGGGCACCTGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGCGGCGGTCACGCTCGATGGC‑GATCGCG‑CATTTGGTCAGGTCGC‑GGCCCATGAAGCGATg                                                                                                                                                                                      >  1:6982/1‑239 (MQ=255)                                                aTTTCGTCAGATGGGTAGCGAGGAACAAGAAGCGAAATTAGTTGCCGATCATTTAATCGCGGCAAACCTGGCAGGGCATGATTCACATGGTATTGGCATGATCCCAAGCTATGTACGCTCCTGGAGTCAGGGGCACCTGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGCGGCGGTCACGCTCGATGGC‑GATCGCG‑CATTTGGTCAGGTCGC‑GGCACATGAAGCGATg                                                                                                                                                                                      <  2:90531/238‑1 (MQ=255)                                                    cGTCAGATGGGTAGCGAGGAACAAGAAGCGAAATTAGTTGCCGATCATTTAATCGCGGCAAACCTGGCAGGGCATGATTCACATGGTATTGGCATGATCCCAAGCTATGTACGCTCCTGGAGTCAGGGGCACCTGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGCGGGGGTCACGCTCGCTGGC‑GAACCCG‑CCTTTTGGCAGGGCGC‑GGCACATGACGCGGTGgcgcc                                                                                                                                                                                 >  2:484789/1‑238 (MQ=255)                                                         gATGGGTAGCGAGGAACAAGAAGCGAAATTAGTTGCCGATCATTTAATCGCGGCAAACCTGGCAGGGCATGATTCACATGGTATTGGCATGATCCCAAGCTATGTACGCTCCTGGAGTCAGGGGCACCTGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGGGGCGGTCACGCTCGATTGG‑GATCTCG‑CATTTGGGCAGGTCGC‑GGCCCACGAAACGATGGGGCCGGGGa                                                                                                                                                                            >  1:1093936/1‑239 (MQ=255)                                                         gATGGGTAGCGAGGAACAAGAAGCGAAATTAGTTGCCGATCATTTAATCGCGGCAAACCTGGCAGGGCATGATTCACATGGTATTGGCATGATCCCAAGCTATGTACGCTCCTGGAGTCAGGGGCACCTGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGGGGCGGGCACGCTCGATGGG‑GGGCCCG‑CCTTTGGTGAGGGGGC‑GGGCCATAAAGCGAGCGCGCTggggg                                                                                                                                                                            >  2:499975/1‑238 (MQ=255)                                                         gATGGGTAGCGAGGAACAAGAAGCGAAATTAGTTGCCGATCATTTAATCGCGGCAAACCTGGCAGGGCATGATTCACATGGTATTGGCATGATCCCAAGCTATGTACGCTCCTGGAGTCAGGGGCACCTGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGCGGCGGTCACGCTCGATGGC‑GATCGCG‑CATTTGGTCAGGTCGC‑GGCACATGAAGCGATGGCGCTggggg                                                                                                                                                                            >  1:643172/1‑238 (MQ=255)                                                         gATGGGTAGCGAGGAACAAGAAGCGAAATTAGTTGCCGATCATTTAATCGCGGCAAACCTGGCAGGGCATGATTCACATGGTATTGGCATGATCCCAAGCTATGTACGCCCCTGGAGTCAGGGGCACCTGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGCGGCGGGCCCCCCCCCTGGC‑GAGCGCG‑CACTTGTGCACGGCGG‑GGCCCCTGGAGCGGTGGGGCCggggg                                                                                                                                                                            >  2:74212/1‑238 (MQ=255)                                                              gTAGCGAGGAACAAGAAGCGAAATTAGTTGCCGATCATTTAATCGCGGCAAACCTGGCAGGGCATGATTCACATGGTATTGGCATGATCCCAAGCTATGTACGCTCCTGGAGTCAGGGGCACCTGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGCGGCGGTCCCGCTCGGCGGCGGCACCCG‑C‑CTTGGGCCGGTCGCGGGGCCATGAGGCGCTGGCGCGGGGGATTgg                                                                                                                                                                        >  2:877542/1‑238 (MQ=255)                                                                   gAGGAACAAGAAGCGAAATTAGTTGCCGATCATTTAATCGCGGCAAACCTGGCAGGGCATGATTCACATGGTATTGGCATGATCCCAAGCTATGTACGCTCCTGGAGTCAGGGGCACCTGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGCGGCGGTCACGCTCGATGGC‑GATCGCG‑CATTTGGTCAGGTCGC‑GGCACATGAAGCGATGGCGCTGGGGATTGAGAAAg                                                                                                                                                                   <  2:551010/238‑1 (MQ=255)                                                                    aGGAACAAGAAGCGAAATTAGTTGCCGATCATTTAATCGCGGCAAACCTGGCAGGGCATGATTCACATGGTATTGGCATGATCCCAAGCTATGTACGCTCCTGGAGTCAGGGGCACCTGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGGGGCGGGCACCCTCGATGGC‑GAACGCG‑CATTTTGGTAGGTCGC‑GGCACATGAAACGGTGGGGCTGGGGGTTggggaaag                                                                                                                                                                  >  1:850897/1‑231 (MQ=255)                                                                    aGGAACAAGAAGCGAAATTAGTTGCCGATCATTTAATCGCGGCAAACCTGGCAGGGCATGATTCACATGGTATTGGCATGATCCCAAGCTATGTACGCTCCTGGAGTCAGGGGCACCTGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGCGGCGGGCACGCTCGATGGC‑GATCGCG‑CCTTTTGGCAGGGCGC‑GGGACATGAAGCGAGGGGGCCGGGGGTTGAGaaaagg                                                                                                                                                                 >  1:699259/1‑236 (MQ=255)                                                                        actggagtcGAAAATAGTTGCCGATCTTTTAATCGCGGCAATCCTGTCAGCTCATGATTCACATGGTATTCGCATGATCCCAATCTATGTACAGTCCTCGAGTCAGGGGCACCTGGAAATCAACCTTCATGCGAAAACCGTTAAAGAGGCGGGGGCGGCGGTCACGGTCGATGGC‑GATCGCG‑CATTTGGTCAGGGCGC‑GGCATATGAAGCAATGGCGCTGGGGATTGAGAAAGCGCAt                                                                                                                                                              <  2:1058052/230‑1 (MQ=255)                                                                              agCGAAATTAGTTGCCGATCATTTAATCGCGGCAAACCTGGCAGGGCATGATTCACATGGTATTGGCATGATCCCAAGCTATGTACGCTCCTGGAGTCAGGGGCACCTGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGCGGCGGTCACGCTCGATGGC‑GATCGCG‑CATTTGGTCAGGTCGC‑GGCACATGAAGCGATGGCGCTGGGGATTGAGAAAGCGCATCAGCAc                                                                                                                                                        <  2:553288/238‑1 (MQ=255)                                                                              agCGAAATTAGTTGCCGATCATTTAATCGCGGCAAACCTGGCAGGGCATGATTCACATGGTATTGGCATGATCCCAAGCTATGTACGCTCCTGGAGTCAGGGGCACCTGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGCGGCGGTCACGCTCGATGGC‑GATCGCG‑CATTTGGTCAGGTCGC‑GGCACATGAAGCGATGGCGCTGGGGATTGAGAAAGCGCATCAGCACg                                                                                                                                                       <  1:418498/239‑1 (MQ=255)                                                                                                        tCGCGGCAAACCTGGCAGGGCATGATTCACATGGTATTGGCATGATCCCAAGCTATGTACGCTCCTGGAGTCAGGGGCACCTGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGCGGCGGTCACGCTCGATGGC‑GATCGCG‑CATTTGGTCAGGTCGC‑GGCACATGAAGCGATGGCGCTGGGGATTGAGAAAGCGCATCAGCACGGTATTGCCGCCGTGGCGCTACATAAc                                                                                                                             <  1:606073/239‑1 (MQ=255)                                                                                                         cgcgGCAAACCTGGCAGGGCATGATTCACATGGTATTGGCATGATCCCAAGCTATGTACGCTCCTGGAGTCAGGGGCACCTGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGCGGCGGTCACGCTCGATGGC‑GATCGCG‑CATTTGGTCAGGTCGC‑GGCACATGAAGCGATGGCGCTGGGGATTGAGAAAGCGCATCAGCACGGTATTGCCGCCGTGGCGCTACATAACt                                                                                                                            <  2:1038776/239‑1 (MQ=255)                                                                                                                           gCATGATTCACATGGTATTGGCATGATCCCAAGCTATGTACGCTCCTGGAGTCAGGGGCACCTGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGCGGCGGTCACGCTCGATGGC‑GATCGCG‑CATTTGGTCAGGTCGC‑GGCACATGAAGCGATGGCGCTGGGGATTGAGAAAGCGCATCAGCACGGTATTGCCGCCGTGGCGCTACATAACTCGCATCATATCGGcc                                                                                                             <  1:856714/236‑1 (MQ=255)                                                                                                                                  tCACATGGTATTGGCATGATCCCAAGCTATGTACGCTCCTGGAGTCAGGGGCACCTGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGCGGCGGTCACGCTCGATGGC‑GATCGCG‑CATTTGGTCAGGTCGC‑GGCACATGAAGCGATGGCGCTGGGGATTGAGAAAGCGCATCAGCACGGTATTGCCGCCGTGGCGCTACATAACTCGCATCATATCGGCCGTATCGGTTa                                                                                                   <  2:305889/239‑1 (MQ=255)                                                                                                                                    acaTGGTATTGGCATGATCCCAAGCTATGTACGCTCCTGGAGTCAGGGGCACCTGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGCGGCGGTCACGCTCGATGGC‑GATCGCG‑CATTTGGTCAGGTCGC‑GGCACATGAAGCGATGGCGCTGGGGATTGAGAAAGCGCATCAGCACGGTATTGCCGCCGTGGCGCTACATAACTCGCATCATATCGGCCGTATCGGTTACt                                                                                                 <  1:415496/239‑1 (MQ=255)                                                                                                                                    acaTGGTATTGGCATGATCCCAAGCTATGTACGCTCCTGGAGTCAGGGGCACCTGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGCGGCGGTCACGCTCGATGGC‑GATCGCG‑CATTTGGTCAGGTCGC‑GGCACATGAAGCGATGGCGCTGGGGATTGAGAAAGCGCATCAGCACGGTATTGCCGCCGTGGCGCTACATAACTCGCATCATATCGGCCGTATCGGTTACt                                                                                                 <  1:499975/239‑1 (MQ=255)                                                                                                                                    acaTGGTATTGGCATGATCCCAAGCTATGTACGCTCCTGGAGTCAGGGGCACCTGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGCGGCGGTCACGCTCGAAGGC‑GATCGCG‑CATTTGGTCAGGTCGC‑GGCACATGAAGCGATGGCGCTGGGGATTGAGAAAGCGCATCAGCACGGTATTGCCGCCGTGGCGCTACATAACTCGCATCATATCGGCCGTATCGGTTACt                                                                                                 <  1:775059/239‑1 (MQ=255)                                                                                                                                         gTATTGGCATGATCCCAAGCTATGTACGCTCCTGGAGTCAGGGGCACCTGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGCGGCGGTCACGCTCGATGGC‑GATCGCG‑CATTTGGt                                                                                                                                                                                                               <  1:958157/125‑1 (MQ=255)                                                                                                                                          tATTGGCATGATCCCAAGCTATGTACGCTCCTGGAGTCAGGGGCACCTGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGCGGCGGTCACGCTCGATGGC‑GATCGCG‑CATTTGGTCAGGTCGC‑GGCACATGAAGCGATGGCGCTGGGGATTGAGAAAGCGCATCAGCACGGTATTGCCGCCGTGGCGCTACATAAc                                                                                                                             <  1:206197/205‑1 (MQ=255)                                                                                                                                          tATTGGCATGATCCCAAGCTATGTACGCTCCTGGAGTCAGGGGCACCTGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGCGGCGGTCACGCTCGATGGC‑GATCGCG‑CATTTGGTCAGGTCGC‑GGCACATGAAGCGATGGCGCTGGGGATTGAGAAAGCGCATCAGCACGGTATTGCCGCCGTGGCGCTACATAACTCGc                                                                                                                         <  1:982297/209‑1 (MQ=255)                                                                                                                                          tATTGGCATGATCCCAAGCTATGTACGCTCCTGGAGTCAGGGGCACCTGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGCGGCGGTCACGCTCGATGGC‑GATCGCG‑CATTTGGTCAGGTCGC‑GGCACATGAAGCGATGGCGCTGGGGATTGAGAAAGCGCATCAGCACGGTATTGCCGCCGTGGCGCTACATAACTCGCATCATATCGGCCGTATCGGTTACTGGGCg                                                                                            <  1:854607/238‑1 (MQ=255)                                                                                                                                          tATTGGCATGATCCCAAGCTATGTACGCTCCTGGAGTCAGGGGCACCTGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGCGGCGGTCACGCTCGATGGC‑GATCGCG‑CATTTGGTCAGGTCGC‑GGCACATGAAGCGATGGCGCTGGGGATTGAGAAAGCGCATCAGCACGGTATTGCCGCCGTGGCGCTACATAACTCGCATCATATCGGCCGTATCGGTTACTGGGCg                                                                                            <  2:643172/238‑1 (MQ=255)                                                                                                                                            ttGGCATGATCCCAAGCTATGTACGCTCCTGGAGTCAGGGGCACCTGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGCGGCGGTCACGCTCGATGGC‑GATCGCG‑CATTTGGTCAGGTCGC‑GGCACATGAAGCGATGGCGCTGGGGATTGAGAAAGCGCATCAGCACGGTATTGCCGCCGTGGCGCTACATAACTCGCATCATATCGGCCGTATCGGTTACTGGGCGGAg                                                                                         <  1:561357/239‑1 (MQ=255)                                                                                                                                                    aTCCCAAGCTATGTACGCTCCTGGAGTCAGGGGCACCTGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGGGGCGGGCACCCCCGATGGC‑GATCGCG‑CATTTGGGCCGGGGGC‑GGCACCTGAAGCGAGGGGGCCGGGGGTTTAGAAAGGGCCTCAGCACGGGATTTCCGCCGGGGCGCGACATAACTCCCACCACAACGGCCGTATCGGGTGCTTGGGGGGGCAGtgtgg                                                                                 >  2:58087/1‑238 (MQ=255)                                                                                                                                                    aTCCCAAGCTATGTACGCTCCTGGAGTCAGGGGCACCTGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGCGGCGGGCCAGCTCGATGGC‑GATCGCG‑CCTTTGGTCAGGGCGC‑GGGCCATGAAGAGGTGGCGCTGGGGATTGGGAAAGCGCATCAGCACGGTATTTGCGCCGTGGGGCTCCATTACACCCATCCTACAGGCCGTATCGGTTACTGGGGGGAGGAGggtgt                                                                                 >  2:764108/1‑238 (MQ=255)                                                                                                                                                            cTAGGTACGCTCCTGGAGTCAGGGGCACCTGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGCGGCGGTCACGCTCGATGGC‑GATCGCG‑CATTTGGTCAGGGCGC‑GGCACATGAAGCGATGGCGCTGGGGATTGAGAAAGCGCATCAGCACGGTATTGCCGCCGTGGCGCGACATAACTCGCATCATATCGGCCGTATCGGTTACTGGGCGGa                                                                                          <  1:534212/222‑1 (MQ=255)                                                                                                                                                                      tCCTGGAGTCAGGGGCACCTGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGCGGGGGGCACGCCCGATGGC‑GATCGCG‑CCTTTGGGCCGGGCGC‑GGGACATGAAGCGATGGCGCTGGGGGGTGAGAAAGCGCATCAGCACGGTATTGGCGCCGGGGCGCGACAATACTCGCATCCTATCGGGCGGGTCCGGTACTGGGGGGGGCCGGGGGCAGGGGCggggggtgtg                                                                 >  2:559497/1‑231 (MQ=255)                                                                                                                                                                      tCCTGGAGTCAGGGGCACCTGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGCGGCGGTCACGCTCGATGGC‑GATCGCG‑CATTTGGTCAGGTCGC‑GGCACATGAAGCGATGGCGCTGGGGATTGAGAAAGCGCATCAGCACGGTATTGCCGCCGTGGCGCTACATAACTCGCATCATATCGGCCGTATCGGTTACTGGGCGGAGCAGTGTGCAGCGGCGGGGTTTGtctc                                                               <  1:722284/239‑1 (MQ=255)                                                                                                                                                                      tCCTGGAGTCAGGGGCACCTGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGCGGCGGGCCCGCCCCGAGGC‑GAACGCGCCTTTTGGTC‑GGGCGC‑GGGCCATGAAGCGGAGGGGCCGGGGGGTGGGAAAACGGCTCAGCACGGGGTTGGCGCCCGGGGGGTTCATAAACCGCCTCCTATCGGCCGGATCGGTTTCTGGGGGGGGCCGCGGGGAGCGggggggggtggg                                                                 >  2:669967/1‑231 (MQ=255)                                                                                                                                                                             gTCAGGTGCACCTGCAAATTAACCATCATGCCAAACCCGTTAAAGAGGCGGGGGCGGCGGTCACGCTCGATGGC‑GATCGCG‑CATTTGGTCAGGTCGC‑GGCACATGAAGCGATGGCGCTGGGGATTGAGAAAGCGCATCAGCACGGTATTGCCGCCGTGGCGCTACATAACTCGCATCATATCGGCCGTATCGGTTACTGGGCGGAGCAGTGTGCAGCGGCGGGGTTTGTCTCTATCCa                                                         <  2:371621/238‑1 (MQ=255)                                                                                                                                                                             gTCAGGGGCACCTGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGCGGCGGTCACGCTCGATGGC‑GATCGCG‑CATTTGGTCAGGTCGC‑GGCACATGAAGCGATGGCGCTGGGGATTGAGAAAGCGCATCAGCACGGTATTGCCGCCGTGGCGCTACATAACTCGCATCATATCGGCCGTATCGGTTACTGGGCGGAGCAGTGTGCAGCGGCGGGGTTTGTCTCTATCCa                                                         <  1:484789/238‑1 (MQ=255)                                                                                                                                                                             gTCAGGGGCACCTGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGCGGCGGTCACGCTCGATGGC‑GATCGCG‑CATTTGGTCAGGTCGC‑GGCACATGAAGCGATGGCGCTGGGGATTGAGAAAGCGCATCAGCACGGTATTGCCGCCGTGGCGCTACATAACTCGCATCATATCGGCCGTATCGGTTACTGGGCGGAGCAGTGTGCAGCGGCGGGGTTTGTCTCTATCCa                                                         <  1:764108/238‑1 (MQ=255)                                                                                                                                                                                aGGGGCACCTGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGGGGCGGGCACGCCCGACGGC‑GAGCGCG‑CATTTTGCCAGGGCGC‑GGCACATGAAGCGAGGGCGCTGGGGATTGGGAAAGCGCCTCCGCACGGTAGTGACGCCGGGGCGCTGCCAAACTCCCATCATATCCGCCGCATCGGTTACTTGGGGGAGCCGGGGGGAGCGGCGGGGGTGGTCTCTATcacccat                                                     >  2:37490/1‑233 (MQ=255)                                                                                                                                                                                aGGGGCACCTGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGCGGCGGGCAACCCCGATGGC‑GATCCCG‑CCTTTGGGCAGGGCCG‑GGCACATGAAACGATGGCGCGGGGGGGTGAGAAAACGCCGCAGCACCGGATTTCCGCCGGGGCGCTACATAAACCGCATCCAATCGGGCGGATAGGTTACGTGGCGGGGCAGAGTGGAGCGGCGGGGGTTTtctctctctcacct                                                     >  2:1115487/1‑233 (MQ=255)                                                                                                                                                                                            aaaTTAACCATCATGCCAAAACCGTTAAAGGGGCGGGGGCGGCGGTCACGCTCGATGGC‑GATCGCG‑CATTGGGTCAGGTCGC‑GGCACATGAAGCGATGGCGCTGGGGATTGAGAAAGCGCATCAGCACGGTATTGCCGCCGTGGCGCTACATAACTCGCATCATATCGGCCGTATCGGTTACTGGGCGGAGCAGTGTGCAGCGGCGGGGTTTGTCTCTATCCACTTTGTTAGCGTGGt                                          <  2:81468/238‑1 (MQ=255)                                                                                                                                                                                            aaaTTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGCGGCGGTCACGCTCGATGGC‑GATCGCG‑CATTTGGTCAGGTCGC‑GGCACATGAAGCGATGGCGCTGGGGATTGAGAAAGCTCATCAGCACGGTATTGCCGCCGTGGCGCTACATAACTCGCATCATATCGGCCGTATCTGTTACTGGGCGGAGCAGTGTGCAGCGGCGGGGTTTGTCTCTATCCACTTTGTTAGCGTGGt                                          <  2:100025/238‑1 (MQ=255)                                                                                                                                                                                            aaaTTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGCGGCGGTCACGCTCGATGGC‑GATCGCG‑CATTTGGTCAGGTCGC‑GGCACATGAAGCGATGGCGCTGGGGATTGAGAAAGCGCATCAGCACGGTATTGCCGCCGTGGCGCTACATAACTCGCATCATATCGGCCGTATCGGTTACTGGGCGGAGCAGTGTGCAGCGGCGGGGTTTGTCTCTATCCACTTTGTTAGCGTGGt                                          <  1:747258/238‑1 (MQ=255)                                                                                                                                                                                            aaaTTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGCGGCGGTCACGCTCGATGGC‑GATCGCG‑CATTTGGTCAGGTCGC‑GGCACATGAAGCGATGGCGCTGGGGATTGAGAAAGCGCATCAGCACGGTATTGCCGCCGTGGCGCTACATAACTCGCATCATATCGGCCGTATCGGTTACTGGGCGGAGCAGTGTGCAGCGGCGGGGTTTGTCTCTATCCACTTTGTTAGCGTGGt                                          <  1:767429/238‑1 (MQ=255)                                                                                                                                                                                            aaaTTAACCATCAGGCAAAAACGGTTAAAGAGGCGGGGGCGGCGGTCACGCTCGATGGC‑GATCGCG‑CATTTGGTCAGGTCGC‑GGCACATGAAGCGATGGCGCTGGGGATTGAGAAAGCGCATCAGCACGGTATTGCCGCCGTGGCGCTACATAACTCGCATCATATCGGCCGTATCGGTTACTGGGCGGAGCAGTGTGCAGCGGCGGGGTTTGTCTCTATCCACTTTGTTAGCGTGGt                                          <  2:396679/238‑1 (MQ=255)                                                                                                                                                                                            aaaTGAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGCGGCGGTCACGCTCGATGGC‑GATCGCG‑CATTTGGTCAGGTCGC‑GGCACATGAAGCGATGGCGCTGGGGATTGAGAAAGCGCATCAGCACGGTATTGCCGCCGTGGCGCTACATAACTCGCATCATATCGGCCGTATCGGTTACTGGGCGGAGCAGTGTGCAGCGGCGGGGTTTGTCTCTATCCACTTTGTTAGCGTGGt                                          <  2:850897/238‑1 (MQ=255)                                                                                                                                                                                               ttAACCATCATGCGAAAACCGTTAAAGAGGCGGGGGCGGCGGGCACGCTCGATGGC‑GATCGCG‑CATTTGGTCAGGTCGC‑GGCACATGAAGCGATGGCGCTGGGGATTGAGAAAGCGCATCAGCACGGTATTGCCGCCGTGGCGCTACATAACTCGCATCATATCGGCCGTATCGGTTACTGGGCGGAGCAGTGTGCAGCGGCGGGGTTTGTCTCTATCCACTTTGTTAGCGTGGTCGGt                                      <  2:851714/239‑1 (MQ=255)                                                                                                                                                                                                                    atAAAGAGGCGGGGGCGGCGGGCACGGTCGAGGGC‑GAGCGCG‑CATTTGGTCAGGTCGC‑GGCACATGAAGCGATGGCGCTGGGGATTGAGAAAGCGCACCAGTACGGTATTGCCGCCGTGGCGCTACATAACCCGCATCATATCGGCCGTATCGGTTACTGGGCGGAGCAGTGTGCAGGGGCGGGGTTTGTCTCTATCCACTTTTTTAGCGTGGTCGGTATTCCAATGGTCGCGCCGTTc                 <  2:545498/238‑1 (MQ=255)                                                                                                                                                                                                                      aaaGAGGCGGGGGCGGCGGTCACGCTCGATGGC‑GATCGCG‑CATTTGGTCAGGTCGC‑GGCACATGAAGCGATGGCGCTGGGGATTGAGAAAGCGCATCAGCACGGTATTGCCGCCGTGGCGCTACATAACTCGCATCATATCGGCCGTATCGGTTACTGGGCGGAGCAGTGTGCAGCGGCGGGGTTTGTCTCTATCCACTTTGTTAGCGTGGTCGGTATTCCAATGGTCGCGCCGTTCCa               <  1:662848/239‑1 (MQ=255)                                                                                                                                                                                                                                    ggcggTCCCGCTCGATGGC‑GATCGCG‑CATTTGGTCAGGTCGC‑GGCACATGAAGCGATGGCGCTGGGGATTGAGAAAGCGCAGCAGCCCGGTATTGCCGCCGTGGCGCTACATAACTCGCATCATATCGGCCGTATCGGTTACTGGGCGGAGCAGTGTGCAGCGGCGGGGTTTGTCTCTATCCACTTTGTTAGCGTGGTCGGTATTCCAATGGTCGCGCCGTTCCACGGTCGCGACAGc  <  2:699259/238‑1 (MQ=255)                                                                                                                                                                                                                                    ggcggTCACTCTGGAGGGC‑GATCGCG‑CATTTGGGCAGGTCGC‑GGCACATGAAGCGATGGCGCTGGGGATTGAGAAAGCGCATCAGCACGGTATTGCCGCCGTGGCGCTACATAACTCGCATCATATCGGCCGTATCGGTTACTGGGCGGAGCAGTGTGCAGCGGCGGGGTTTGTCTCTATCCACTTTGTTAGCGTGGTCGGTATTCCAATGGTCGCGCCGTTCCACGGTCGCGACAGc  <  2:466746/238‑1 (MQ=255)                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                            GGTCATCGCTTTGATGCTCAGACGCTGCACAGTTTTATTCAGGCTGTATTTCGTCAGATGGGTAGCGAGGAACAAGAAGCGAAATTAGTTGCCGATCATTTAATCGCGGCAAACCTGGCAGGGCATGATTCACATGGTATTGGCATGATCCCAAGCTATGTACGCTCCTGGAGTCAGGGGCACCTGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGCGGCGGTCACGCTCGATGGC‑GATCGCG‑CATTTGGTCAGGTCGC‑GGCACATGAAGCGATGGCGCTGGGGATTGAGAAAGCGCATCAGCACGGTATTGCCGCCGTGGCGCTACATAACTCGCATCATATCGGCCGTATCGGTTACTGGGCGGAGCAGTGTGCAGCGGCGGGGTTTGTCTCTATCCACTTTGTTAGCGTGGTCGGTATTCCAATGGTCGCGCCGTTCCACGGTCGCGACAGC  >  NC_000913/836360‑836824

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑