BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	3,206,438	G→A	18.1%	intergenic (‑182/‑25)	ttdR ← / → ttdA	transcriptional activator of ttdABT/L‑tartrate dehydratase, alpha subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,206,438	0	G	A	18.1%	205.6 / 16.7	112	intergenic (‑182/‑25)	ttdR/ttdA	transcriptional activator of ttdABT/L‑tartrate dehydratase, alpha subunit
Reads supporting (aligned to +/- strand): ref base G (30/57); new base A (11/9); total (45/67)
Fisher's exact test for biased strand distribution p-value = 1.25e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 113 total aligned reads displayed.

CCAGTACCTGCAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTAGTGG‑TAGTTTGTGGCGG‑T‑GAATTTTTCCAGACAAATACAAAAACTGGA‑GTTGCCA‑TGATGAGCGAAAGTAATAAGC‑AACAGGCAGTGAATAAGTTGACAGAGATTGTCGCT‑AACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGAT‑GCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTC  >  NC_000913/3206214‑3206676

ccAGTACCTGCAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTAGTGG‑TAGTTTGTGGCGG‑T‑GAATTTTTCCAGACAAATACAAAAAc                                                                                                                                                                                                                                       <  2:872787/239‑1 (MQ=255)

    tACCTGCAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTAGTGG‑TAGTTTGTGGCGGCT‑CAATTTTT‑CAGACCAATACAAAAACTCGa                                                                                                                                                                                                                                   >  1:314969/1‑239 (MQ=255)

    tACCTGCAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTAGTGG‑TAGTTTGTGGCGG‑TGGATTTTTTCCATAAAAAAACaaaaatagt                                                                                                                                                                                                                                    >  2:555187/1‑235 (MQ=255)

    tACCTGCAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTAGTGG‑TAGTTTGTGGCGG‑T‑GGATTTTTTCAGACCAATAaaaaaaattgg                                                                                                                                                                                                                                   >  1:975127/1‑234 (MQ=255)

     aCCTGCAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTAGTGG‑TAGTTTGTGGCGG‑T‑GAATTTTTTTAGACAAATACAAAAAATGAA‑g                                                                                                                                                                                                                                 >  1:599583/1‑239 (MQ=255)

     aCCTGCAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTAGTGG‑TAGTTTGTGGCGG‑T‑GAATTTTTTCAGACAAATACAAAAAATaga‑g                                                                                                                                                                                                                                 >  1:480813/1‑239 (MQ=255)

     aCCTGCAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTAGTGG‑TAGTTTGTGGCGG‑T‑GAATTTTTCCAGACAAATACAAAAACTGGA‑g                                                                                                                                                                                                                                 <  1:213386/239‑1 (MQ=255)

        tGCAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTAGTGG‑TAGTTTGTGGCGG‑T‑GAATTTTTTTATACAAATAAAAAAACATGA‑GTTg                                                                                                                                                                                                                              >  1:311670/1‑239 (MQ=255)

        tGCAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTAGTGG‑TAGTTTGTGGCGG‑T‑GAATTTTTTTAGACACATACaaaaaatcgggtt                                                                                                                                                                                                                                >  1:613454/1‑230 (MQ=255)

         gCAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTAGTGG‑TAGTTTGTGGCGG‑T‑GAATTTTTCCAGACAAATACAAAAACTGGA‑GTTGc                                                                                                                                                                                                                             <  1:908141/239‑1 (MQ=255)

                     cTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTAGTGG‑TAGTTTGTGGCGG‑T‑GAATTTTTCCAGACAAATACAAAAACTGGA‑GTTGCCA‑TGATGAGCg                                                                                                                                                                                                                 <  1:81087/238‑1 (MQ=255)

                      tAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTAGTGG‑TAGTTTGTGGCGG‑T‑GAATTTTTCCAGACAAATACAAAAACTGGA‑GTTGCCA‑TGATGAGCGa                                                                                                                                                                                                                <  2:1153003/238‑1 (MQ=255)

                      tAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTAGTGG‑TAGTTTGTGGCGG‑T‑GAATTTTTCCAGACAAATACAAAAACTGGA‑GTTGCCA‑TGATGAGCGa                                                                                                                                                                                                                <  2:206757/238‑1 (MQ=255)

                      tAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTAGTGG‑TAGTTTGTGGCGG‑T‑GAATTTTTCCAGACAAATACAAAAACTGGA‑GTTGCCA‑TGATGAGCGa                                                                                                                                                                                                                <  1:460665/238‑1 (MQ=255)

                        aaGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTAGTGG‑TAGTTTGTGGCGG‑T‑GAATTTTTCCAGACa                                                                                                                                                                                                                                                  <  2:856585/204‑1 (MQ=255)

                             cAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTAGTGG‑TAGTTTGTGGCGG‑T‑GAATTTTTCCAGACAAATACAAAAACTGGA‑GTTGCCA‑TGATGAGCGAAAGtaat                                                                                                                                                                                                         <  2:363801/238‑1 (MQ=255)

                              aGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTAGTGG‑TAGTTTGTGGCGG‑T‑GAATTTTTTCAGACAAAAACAAAAAATTGG‑GTTGTCA‑TTGTGTGGGGAAGgaataa                                                                                                                                                                                                       >  1:522800/1‑239 (MQ=255)

                                   ttCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTAGTGG‑TAGTTTGTGGCGG‑T‑GAATTTTTCCAGACAAATACAAAAACTGGA‑GTTGCCA‑TGATGAGCGAAAGTAATAAGC‑AAc                                                                                                                                                                                                 <  2:864547/239‑1 (MQ=255)

                                         aTCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTAGTGG‑TAGTTTGTGGCGG‑T‑GAATTTTTCCAGACAAATACAAAAACTGGA‑GTTGCCA‑TGATGAGCGAAAGTAATAAGC‑AACAgg                                                                                                                                                                                              <  1:75802/236‑1 (MQ=255)

                                         aTCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTAGTGG‑TAGTTTGTGGCGG‑T‑GAATTTTTCCAGACAAATACAAAAACTGGA‑GTTGCCA‑TGATGAGCGAAAGTAATAAGC‑AACAgg                                                                                                                                                                                              <  2:1186004/236‑1 (MQ=255)

                                         aTCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTAGTGG‑TAGTTTGTGGCGG‑T‑GAATTTTTCCAGACAAATACAAAAACTGGA‑GTTGCCA‑TGATGAGCGAAAGTAATAAGC‑AACAgg                                                                                                                                                                                              <  1:220231/236‑1 (MQ=255)

                                           cGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTAGTGG‑TAGTTTGTGGCGG‑T‑GAATTTTTCCAGACAAATACAAAAACTGGA‑GTTGCCA‑TGATGAGCGAAAGTAATAAGC‑AACAGGCAGTg                                                                                                                                                                                         <  2:118861/239‑1 (MQ=255)

                                           cGTTAATTATCCGCAGTTGGGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTAGTGG‑TAGTTTGTGGCGG‑A‑GAATTTTTTCAGGCAAAAACAAAAACTTGA‑GGTGGCA‑TTGTGTGGGAAAGGAATAAGC‑AAAAggacggg                                                                                                                                                                                         >  1:975497/1‑234 (MQ=255)

                                             ttAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTAGTGG‑TAGTTTGTGGCGG‑T‑GAATTTTTTCAAAACAATACAAAAACATGA‑GTTGCCC‑TGATGAGCGGAAAGAACAAGC‑cacaagaaaggaa                                                                                                                                                                                       >  1:194187/1‑230 (MQ=255)

                                              tAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTAGTGG‑TAGTTTGTGGCGG‑T‑GAATTTTTCCAGACAAATACAAAAACTGGA‑GTTGCCA‑TGATGAGCGAAAGTAATAAGC‑AACAGGCAGTGaa                                                                                                                                                                                       <  1:986923/238‑1 (MQ=255)

                                              tAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTAGTGG‑TAGTTTGTGGCGG‑T‑GAATTTTTCCAGACAAATACAAAAACTGGA‑GTTGCCA‑TGATGAGCGAAAGTAATAAGC‑AACAGGCAGTGaa                                                                                                                                                                                       <  2:142378/238‑1 (MQ=255)

                                               aaTTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTAGTGG‑TAGTTTGTGGCGG‑T‑GAATTTTTCCAGACAAATACAAAAACGGGA‑GTTGCCA‑TGATGAGCGAAAGTAATAAGC‑AACAGGCAGTGAATa                                                                                                                                                                                     <  1:390139/239‑1 (MQ=255)

                                                            tgtgATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTAGTGG‑TAGTTTGTGGCGG‑T‑GAATTTTTCCAGACAAATACAAAAACTGGA‑GTTGCCA‑TGATGAGCGAAAGTAATAAGC‑AACAGGCAGTGAATAAGTTGACAgaga                                                                                                                                                                         <  2:297507/238‑1 (MQ=255)

                                                              tgATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTAGTGG‑TAGTTTGTGGCGG‑G‑GAATTTTTTCAGACAAATACAAAAACTGGA‑GTTGCCA‑TGATGAGCGAAAATAATAAAC‑AAAAGGCAGTGAATAAGTTGACAGAGATTg                                                                                                                                                                      >  1:487376/1‑239 (MQ=255)

                                                                     cgcAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTAGTGG‑TAGTTTGTGGCGG‑T‑GAATTTTTTCTTACAAAAACAAAAAACGGG‑GTTGGCA‑TGGTAGGCGAAAAGAATAAAG‑AAAAAGAAGTGCATAAATTGACAAAGAGTGTTGCT‑aa                                                                                                                                                              >  1:985118/1‑239 (MQ=255)

                                                                     cgcAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTAGTGG‑TAGTTTGTGGCGG‑T‑GAATTTTTTCCCATAAATACAAAAAATGGA‑GTTGGCA‑TGATGTGGGGAAGATATAAAC‑AACAAGCCGGGGATAAATTTATACAGATTTTTGCT‑aa                                                                                                                                                              >  1:43561/1‑239 (MQ=255)

                                                                            tATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTAGTGG‑TAGTTTGTGGCGG‑T‑GAATTTTTTCAAACAAAAACAAAAAAAAGA‑GGTGTCA‑TGATGAGAGGAAAGAATAAAC‑AAAAGGAAAGGGATAAAATGACAGAGAATGGTGCT‑acaatttt                                                                                                                                                        >  1:433037/1‑231 (MQ=255)

                                                                            tATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTAGTGG‑TAGTTTGTGGCGG‑T‑GAATTTTTCCAGACAAATACAAAAACTGGA‑GTTGCCA‑TGATGAGCGa                                                                                                                                                                                                                <  1:1149144/184‑1 (MQ=255)

                                                                                tCGAAAAACATTGCCATTCCCGGCTCTGGCGAAATTTTCATTCTTATTTTCGCGTTGATTCACAAACTGTTAATTACATATTCACGGTGGAAAAAAATAAAAAACCAC‑ATTTTTAGTGG‑TAGTTTGGGGGGG‑T‑AAATTTTTCCAGAAAAATAAAAAAACGGGA‑GTTGCCA‑TGATGAGCAAAATTAAAAAGC‑AACAGGCAGTAAATAAGTTGACAGAGATTGTCGCT‑AACTTTACCGCCa                                                                                                                                                   <  2:277728/239‑1 (MQ=255)

                                                                                    aaaaCATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTAGTGG‑TAGTTTGTGGCGG‑T‑GAATTTTTTCAGACAAAAAAAAAAACATGA‑GTTGGCA‑TGATGAGCGGAAGAAAAAAAA‑AAAAAGCAAGGAAAAAAATGACAGAGAATGTTGCT‑AAATTTACCCCCATGAt                                                                                                                                               >  1:987055/1‑239 (MQ=255)

                                                                                      aaCATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTAGTGG‑‑AGTTTGTGGCGG‑T‑GAATTTTTTTCAACAAATACAAAAAATGGA‑GGTGTCA‑AGATGAGGGGAAGTAATAAGA‑AACAAGCAGTGAATAAATTGACAGAGATTGTCGCT‑AACTTTACCGCCATGAtt                                                                                                                                              >  1:1217691/1‑237 (MQ=255)

                                                                                                     tGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTAGTGG‑TAGTTTGTGGCGG‑T‑TAATTTTTTCTGACAAATAAAAAAAATTGA‑GTTGCCC‑CGTTGAGCGAAAGGAATAAGA‑AACAGGCACTGAATAAAATGACAAAGATTTTCGCT‑ACCTTTACCCCCATGCTTTCTTCCCGGATTccct                                                                                                                              >  1:73837/1‑237 (MQ=255)

                                                                                                     tGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTAGTGG‑TAGTTTGTGGCGG‑T‑GAATTTTTTTAGACAAAAAAAAAAAATGGAGGTTGCCT‑TG‑TGGGGGGAAGGAAAAAAA‑AAAAGGCAATGAATAAGTTGATAAAGAGTTTCGCT‑TAATTTACCGCCATGATTTTTACACGacaggctc                                                                                                                              >  2:624652/1‑232 (MQ=255)

                                                                                                     tGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTAGTGG‑TAGTTTGTGGCGG‑T‑GAATTTTTCCAGACAAATACAAAAACTGGA‑GTTGCCA‑TGATGAGCGAAAGTAATAAGC‑AACAGGCAGTGAATAAg                                                                                                                                                                                   <  1:590123/187‑1 (MQ=255)

                                                                                                       ctttGGCGCAATATTCATTCTTATTTTCGCGTTAATTCACGACCTGTTAATTACATATTCACGGTGACAAAAAATAAAAAACCAC‑ATTTTAAGTGG‑‑AGTTTGTGGCGG‑T‑AAATTTTTCCAAAAAAATAAAAAAACTGGA‑GTTGCCA‑TGATGAGAGAAAGTAATAACC‑AACAGGCAGTAAATAAGTTGACAGAGATGGCCGCT‑AACTTTACCGCCATGATTTCTACCCGAATGCCtgatg                                                                                                                           <  2:1217691/236‑1 (MQ=255)

                                                                                                       ctctGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTAGTGG‑TAGTTTGTGGCGG‑T‑GAATTTTTTCAGAAAAAAACAAAAAATGGA‑GTTGCCA‑TGATGAGGGGAAGAAATAAAA‑AAAAAGCAAGGAAAAAAAAGAGAGAGAATGGCGCG‑AAAATTACAGCGATGAATTTTACCCGaaagacagag                                                                                                                            >  2:1183843/1‑231 (MQ=255)

                                                                                                        tctGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTAGTGG‑TAGTTTGTGGCGG‑T‑GAATTTTTCCAGACAAATACAAAAACTGGA‑GTTGCCA‑TGATGAGCAAAAGAAATAAGC‑AACAGGCAGTGAATAATTTGACAGAGATTGTCGTT‑AACTTTACCGCCATGATTTCTACCCGAATGCCtgatg                                                                                                                           <  1:826841/239‑1 (MQ=255)

                                                                                                          tGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTAGTGG‑TAGTTTGTGGCGG‑T‑GGAAATTTCCCGTAAAATACAAAAAATTGT‑GGTGGTC‑AGATGAGGAAAAGAAATAAAC‑AAAAGGCAGTGAATTAGATGACAGAAAATGTCCCT‑AACTTTTTCGCCCTGCTTTCTTACCGTATACCttattaa                                                                                                                         >  1:800471/1‑236 (MQ=255)

                                                                                                            gcgcAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTAGTGG‑TAGTTTGTGGCGG‑T‑GTAATTTTTCAGACAAATAAAAAAACTGGA‑GTGGCCA‑TGATGAGGGGAAGTAATAAGA‑AACAGGAAGAGCATAAAATGATAGAGATTGTCGCT‑AACTTTACCGCCCAGAATTCTACCCTAATGCCtgatggagt                                                                                                                       >  1:408108/1‑235 (MQ=255)

                                                                                                            gcgcAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTAGTGG‑TAGTTTGTGGCGG‑T‑GAATTTTTTCAGAAAAAAAAAAAAAATGGA‑GTTGCCA‑TGGTGGGCGGAAATAATAAAA‑AAAAAGCAGTTAATAAGTTGGCAGAGAATGTTGCT‑AAATTTTACCCCATAATTTTTAACCCAAATCCtggtggagg                                                                                                                       >  1:431741/1‑235 (MQ=255)

                                                                                                            gcgcAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTAGTGG‑TAGTTTGTGGCGG‑T‑GAATTTTTTCAAAAAAAAAAAAAAACTGGA‑GTTGCCC‑ATATGGGCGGAAGGAATAAAA‑AACAAGAAAGGAAAAAAGTTAAAAAAATTGTTGGTAAACTTTCCGCCCAGGATTTTTACCCGATTGccgatagagg                                                                                                                        >  1:581942/1‑231 (MQ=255)

                                                                                                            gcgcAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTAGTGG‑TAGTTTGTGGCGG‑A‑GGATTTTTTCAGACAAAAAAAAAAAATGGA‑GTTGGCA‑TGTTGAGCGAAAGTAATAAAC‑AACCAGCAGGGAATAAATTGACAGAGAATGTCGCT‑AAATTTAACGCCCTGATTTCTAACCGAATGCCtgattgagt                                                                                                                       >  1:102849/1‑234 (MQ=255)

                                                                                                             cgcAATATTCATTCTTATTTTCGCGTTGATTCACGAACTTTTAATTACATATTCACGGTGGCAAAAAATAAAAAACCAC‑ATTTTTAGTGG‑TAGTTTGTGGGGG‑T‑GAATTTTTCCAGACAAATACAAAAACTGGA‑GTTGCCA‑TGATGAGCGAAAGTAATAAGC‑AACAGGCAGTGAATAAGTTGACAGAGATTGTCGCT‑AACTTTAc                                                                                                                                                        <  1:572396/205‑1 (MQ=255)

                                                                                                             cacAATATTCATTCTTATTTTCGCGTTGATACACGAACTTTTAATTACATATTCACGGTGGAAAAAAATATAAAACCAC‑ATTTTTAGTGG‑TAGTTTGGGGCGG‑T‑GAATTTTTCCAGACAAATACAAAAACGGGA‑GTTGCCA‑TGATGAGCGAAAGTAATAAGC‑AACAGGCAGTGAATAAGTTGACAGAGATTGTCGCT‑AACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACgt                                                                                                                       <  2:863678/236‑1 (MQ=255)

                                                                                                             cacAAAATTCATTCTTATTTTGGCGTTAATTCACGAACTGTTAATTACATAGTCAGGGGGGCAAAAAATATAAAACCAC‑ATTTTTAGTGG‑AAGTTGGGGGGGG‑T‑GAATTTTTCCACAAAAATACAAAATCTGGT‑GTTCAGA‑AAGAGACGGAAAAAAACAAGC‑AACAGGTGGAGAATAAGAAGAAAAAGATTGTGGCA‑ATATTTACCGCCATGATTTCTACCCGAATGCCTGATGACgt                                                                                                                       <  2:796575/236‑1 (MQ=255)

                                                                                                             caaaaTATCCATGCTTATTTCGACTTTGATGCAGCAATTGTAAATGACATTTTCACAGTGAAAAAAAAAATAAAACCAC‑ATTGAGAGTGG‑TAGTTGTGGGCGG‑T‑AAATTTTTCAAAACAAAAAAAAAAAAAGGA‑TTTGCGA‑TGATGATAAAAAGAAATAAGA‑AACAGGCGGTAAATAATTGGACAGGGTTTGTCCCA‑AACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACgt                                                                                                                       <  2:680323/235‑1 (MQ=255)

                                                                                                              gcAATATTCATTCTTATTTTCGCTTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTAGTGG‑TAGTTTGTGGCGG‑T‑AAATTTTTCCAGAAAAATACAAAAACTGGA‑GTTGCCA‑TGATGAGCAAAAGTAATAAGC‑AACAGGCAGTAAATAAGTTGACAGAGATTGTCGCT‑AATTTTACCGCCATGATTTCTACCCGAATGCCTGATGACgtg                                                                                                                      <  1:292805/238‑1 (MQ=255)

                                                                                                              aaaaTATTAATTCTTATTTTGGCGTTGATTCACGATTTGTTAATTACAGATCCAGGGTGGAAAAAAATAAAAAACAAC‑ATTTTGAGTGG‑TAGTTTGTGGCGG‑T‑GAATTTTCCCAGACAAATACAAAAACAGGA‑GTTGCTA‑TGAGGAGCGAAATAAATAAGC‑ACCGGGAAGAGAATAAGTTGACAGATATTGTCGTT‑AACTTTCCCGCCATGATTTCTACCCGAATGCCTGATGACgtg                                                                                                                      <  1:904719/236‑1 (MQ=255)

                                                                                                                  tatttattTTTTTTTTCGCATTAATTCACTAAATTTTAATAACATATACAGGGTGGAAAAAAATAAAAATCCAG‑ATTATAGATGG‑GTTTTGGGGGGAG‑T‑GATTTTTTGCAGAGAAATAAAAATAGGGGG‑GTTGCGT‑AGAGGAGTAAAAGTAATAAGA‑AACGGGGTGTAGATAGGTAGAAAAGAATTGTCGGT‑AGCTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGa                                                                                                                 <  2:880155/239‑1 (MQ=255)

                                                                                                                  tatTCATTTTTAGTTTCGCGTTGATTCACGAACTGTAAATTACATATTCGCGGGGGCAAAAAATATAAAACCAC‑ATTTTTAGTGT‑TGGTTTGGGGCGG‑T‑AAATTTTCCCAGACAAAAACAAAAACTGGA‑GTTGCCA‑TAATGGGGGAAAATAATAAGA‑AACGGGCAGTAAATAAGTTGACAGAGTTTGTCGCT‑AACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGa                                                                                                                 <  1:1115247/239‑1 (MQ=255)

                                                                                                                  aattcattcTTATTTTCGCGTGGATTCATGAACTGTTAAGTACAGATTCACGGGGGCAAAAAATATAAACCCAC‑ATTTTTAGTGG‑TAGTTTGTGGCGG‑T‑AAATTTTTCCAGACAAAGACAAAAACTGGA‑GTTGCCT‑TGATGAGCGAAAGTAATAAGC‑ACCAGGCAGTGAATAAGTTGACAGAGATTGTCGTT‑AACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGa                                                                                                                 <  1:911028/238‑1 (MQ=255)

                                                                                                                   attcattcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTAGTGG‑TAGTTTGTGGCGG‑T‑GAATTTTTCCAGACAAATACAAAAACTGGA‑GTTGCCA‑TGATGAGCGAAAGTAATAAGC‑AACAgg                                                                                                                                                                                              <  1:872637/162‑1 (MQ=255)

                                                                                                                    ttcattcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTAGTGG‑TAGTTTGTGGCGG‑T‑GAATTTTTTCAGACAAATACAAAAAATGGA‑GTTTCCA‑TGATTGACGAAAGTAATAAAG‑AACAGGCAATGAATAAGTTGACAGAGATTGTCCCT‑AACTTTACCCCCCTGATTTTTTTCCGCAAGCATTCTGACGGGGTGGATa                                                                                                               >  1:769192/1‑239 (MQ=255)

                                                                                                                           ttATTTTCGCGTTGATTCACGAACTTTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTAGTGG‑TAGTTTGTGGCGG‑T‑GAATTTTTCCAGAAAAATACAAAAACTGGA‑GTTGCCA‑TGATGAGCGAAAGTAATAAGC‑AACAGGCAGTGAATAAGTTGACAGAGATTGTCGCT‑AACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTaaa                                                                                                        <  1:939662/239‑1 (MQ=255)

                                                                                                                            tATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTAGTGG‑TAGTTTGTGGCGG‑T‑GAATTTTTTTAAAAAAAAAAAAAAAATGGA‑GTTGTTA‑TTATTAGCGGAAATAAAAAAC‑AAAAAGGAGTGAAAAAATTGACAGAGATTGTCGCT‑AAAATTACACACATCATTTTTAAACGAATGCCTGATGACATGTGGGGTAAAACaaaa                                                                                                       >  2:461981/1‑239 (MQ=255)

                                                                                                                            tATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTAGTGG‑TAGTTTGTGGCGG‑T‑GAATTTTTTCCAACAAAAACAAAAAATTGA‑GGTGTCC‑TTGTGAGGGGAAAGAATAAAA‑CACAAGCAGTGGATAAATTGACAGAGATTGTCGCT‑AAATTTACCGCCACGATTTCTTCCCCAATGCCTGATGGCGTGGGGGATTAACTaaaa                                                                                                       >  2:680575/1‑239 (MQ=255)

                                                                                                                              ttttCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTAGTGG‑TAGTTTGTGGCGG‑T‑GAAATTTTTTCGACAAAAACAAAAAATGGA‑GTTGCCA‑TGATGAGCGAAAGGAATAAAC‑AAAAGGCAATGAATAAATTGACAGAGAATGTCGCT‑AACTTTACCGCCATGATTTTTTCCCGAAAGCCTGATGACGAGGTGGGtaaaataaaaaa                                                                                                     >  1:1095181/1‑237 (MQ=255)

                                                                                                                              ttttCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTAGTGG‑TAGTTTGTGGCGG‑T‑GAAATTTTTCAGACAAATAAAAAAAATGGA‑GGTGCCC‑TGATGAGCGGGAGTAATAAGC‑AACACGCAGTGAATAAAGTGACAGAGATTGTCGCT‑AACTTTACCGCCATGATTTTTACCCGAATGCCTGATGACGTGGTGGATAAACTaaaaaa                                                                                                     >  1:866702/1‑237 (MQ=255)

                                                                                                                                     gTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTAGTGG‑TAGTTTGTGGCGG‑T‑GAATTTTTTCAAACAAAAACAAAAAATGGA‑GTTTGCA‑TGATGAGGGGAAGTAATAAAC‑AAAAGGCAGGGAAAAAGATGACAGAGATTGTCGGC‑AAATTTTCCGCCCTGAATTTTACCCCAAAACCTTGTGTCGTGGGGGGTAAACTTAAACAACTAAAg                                                                                              >  2:939662/1‑239 (MQ=255)

                                                                                                                                              cGAACTTTAAATAACATATTCGCGGTGGAAAAAAATATAAAACCAC‑ATTTTTAGTGG‑TATTTTGTGGCGG‑T‑AAATTTTTCCAGAAAAATAAAAAAACGGGA‑GTTGCCA‑TGATGAGCGAAAGTAATAAGC‑AACAGGCAGTGAATAAGTTGACAGAGATTGTCGCT‑AACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGAT‑GCCGaa                                                                                    <  2:307624/239‑1 (MQ=255)

                                                                                                                                                   ttttATTTAAATATGCACGTTAGCAAAAAATATAAACCCAC‑A‑TTTTGGTGGTTAGTTTGTGGGGG‑T‑AATTTTTTCCAGAAAAATACAAAACCTGGT‑GTTGCCA‑TGATGAGCAAAAGTAAAAAGC‑AAGAGACAGTAAATAAGTTGACAGGAATTGTCGCT‑ATCTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGAT‑GCCGAAAcgtc                                                                               <  2:54715/237‑1 (MQ=255)

                                                                                                                                                   tGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTAGTGG‑TAGTTTGTGGCGG‑T‑GAATTTTTTCAAACAAATAAAAAAAAATGA‑GGTTCCA‑TGATGTGCGAAAGAAAAAAAC‑AACAAGCAGTGAAAAAGTTGGCAAAGATTTTCGCT‑AACTTTATCCCCATGATTTTTACCCGAAAGCCTGATGTAGTGGTGGGTAAAAAAAAACAGCTAAAAAAT‑GCCGcaaact                                                                                >  2:70247/1‑235 (MQ=255)

                                                                                                                                                     ttAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTAGTGG‑TAGTTTGTGGCGG‑G‑GAAATTTTTCAAACAAATAAAAAAACTGGA‑GTTTGCC‑TGGTGGGCGACAGTAATAAGC‑AACAGGCAATGAATAAGTTGACAGAGATTGTCGCC‑CACTTTAACACCACTAATTTTACCCGAACACCTGATTTCGTGGTGGGTAAACTAAAAAAGCCCACAGAT‑GCCGAAAcgccc                                                                              >  2:113577/1‑235 (MQ=255)

                                                                                                                                                     ttAATTACATATTCACGGTGGAAAAAAAAAAAAAACAAC‑ATTTTTAGTGG‑TAGTTGGTGGGGG‑G‑GAATTTTTCAAAAAAAATAAAAAAACTGGA‑GTTGCCA‑TGATGAGCGAAAGTAATAAGC‑AACAGGCAGTAAATAAGTTAACAGAGATTGTCGCT‑AACTTTACCGCCATGATTTCTACCCGAATGCCtgatg                                                                                                                           <  2:511277/194‑1 (MQ=255)

                                                                                                                                                       aaTTACATATTCAGGGTGACAAAAAATAAAAAACCAA‑ATTTTTAGTGG‑TAGTTTGTGGGGG‑T‑GAATTTTTCCAGAAAAATACAAAAACGGGA‑GTTGCCA‑TGATGAGCGAAAGTAAAAAGC‑AACAGGCAGTGAATAAGTTGACAGAGATTGTCGCT‑AACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGAT‑GCCGAAACGtcgtcg                                                                           <  2:522800/239‑1 (MQ=255)

                                                                                                                                                       aaTTACATATGCACGGTGGCAAAAAATAAAAAACCAC‑ATTTTTAGTGG‑TATTTTGGGGCGG‑T‑GAATTTTTCCAGACAAATACAAAAACTGGA‑GTGGCCA‑TGATGAGCAAAAGTAATAAGC‑AACAGGCAGTGAATAAGTTGACAGAGATTGTCGCT‑AACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGAT‑GCCGAAACGtcgtcg                                                                           <  1:1006830/239‑1 (MQ=255)

                                                                                                                                                            aatatTCACGGGGGAAAAAAATATAAAACAAC‑ATTTTTAGTGG‑TAGTTTGTGGCGG‑T‑GAATTTTTCCAGAAAAAAAAAAAAACTGGA‑GTTGCCA‑TGATGAGCGAAAGTAATAAGC‑AACAGGCAGTGAATAAGTTGACAGAGATTGTCGCT‑AACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGAT‑GCCGAAACGTCGTCGATggg                                                                      <  2:480813/238‑1 (MQ=255)

                                                                                                                                                             atatTCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTAGTGG‑TAGTTTGTGGGGG‑T‑GAATTTTTCCAGACAAATACAAAAACTGGA‑GTTGCCA‑TGATGAGCAAAAGTAATAAGC‑AACGGGCAGTGAATAGTTTGACAGAAATTGTCGCT‑AACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGa                                                                                                                 <  1:799563/196‑1 (MQ=255)

                                                                                                                                                              tatTCAGGGTGGAAAAAAATATAAAACACA‑ATTTTTAGGGG‑TAGTTTGTGGCGG‑T‑GAATTTTTCCAGACAAATAAAAAAACTGGA‑GTTGCCA‑TGATGAGCAAAAGTAAAAAGC‑AACAGGCATTGAATAAGTTGACAGAGATTGTCGAT‑AACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGAT‑GCCGAAACGTCGTCGATGGGGa                                                                    <  1:425040/239‑1 (MQ=255)

                                                                                                                                                              tatTCACGGTGGCAAAAAATATAAAACCAC‑ATTTTAATGGG‑TAGTTTGTGGCGG‑T‑GAATTTTTCCAGACAAATACAAAAACTGGA‑GTTGCCA‑TGATGAGCGAAAGTAATAAGC‑AACAGGCAGTGAATAAGTTGACAGAGATTGTCGCT‑AACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGAT‑GCCGAAACGTCGTCGATGGGGa                                                                    <  1:1130141/239‑1 (MQ=255)

                                                                                                                                                              tatTCACGGGGGAAAAAAATAAAAACCCAC‑ATTTTTAGTGG‑TATTTTGTGGCGG‑T‑GAATTTTTCCAGAAAAAAACAAAAACTGGA‑GTTGCCA‑TGATGAGCAAAAGTAATAAGC‑AACAGGAAGTGAATAAGTTGACAGAGATTGTCGCT‑AACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGAT‑GCCGAAACGTCGTCGATGGGGa                                                                    <  1:532241/239‑1 (MQ=255)

                                                                                                                                                               atTCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTAGTGG‑TAGTTTGTGGCGG‑T‑GAATTTTTTCCGACAAAAACAAAAACTGGG‑GTTTCCC‑TGATGAGCGAAAGTAATAAAC‑AACAGGAAGAGAATAAGTTGACAGAGATTGTCGCT‑AACTTTAACGCCCTGATTTTTACCCCAATGCCTGATGACGGGGGGGATAAACTAAAAAAAATAAAGGATGGCCGAAA‑ATCGTCGGTggggga                                                                   >  1:262041/1‑237 (MQ=255)

                                                                                                                                                               atTCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTAGTGG‑TAGTTTGTGGCGG‑T‑GAATTTTTTCAGACAAAAAAAAAAAATGGG‑GGTGCCA‑TGGTGAGCGAAAGGAATAAGC‑AACAGGCAGTGAATAAATTGACAGAGATTTTCGCT‑TACTTTTCCCCCCTGATTTCTTACCCAAAGCCTGATGACGTGGGGGGTAAACTAAAACAACTAAAAGAA‑TCCCGAACAGCGTCGGTggggga                                                                   >  1:232164/1‑237 (MQ=255)

                                                                                                                                                               atTCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTAGTGG‑TAGTTTGTGGCGG‑T‑GAATTTTTTCAAACAAAAACAAAAAATGGA‑GTTGGCC‑TGATGTGGGAAATGAATAAAA‑AACAAGCAGTGAAAAAGATGACAGAGAGTTTCGCT‑AACTTTTACGCCCTGATTTCTACCCGAATTCCTGATGACGGGGGGGGTTAAATAAAACAGATAAAAGAA‑GCCGCAACGTCCTCGCTgggggc                                                                   >  2:1006830/1‑237 (MQ=255)

                                                                                                                                                                   caggTGGAAAAAAATAAAAAACCAA‑ATTGTTATTGT‑TGGTTGGTGGCGG‑T‑AAATTTTTCAAAAAAAATACAAAAACTGGA‑GTTCCAA‑TGATGAGCGAAAGAAAAAAGC‑AACAGGCGGTGAATAAGTGGAAAGAGATTTCCGTT‑AATTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGAT‑GCCGAAACGTCGTCGATGGGGAAAAtt                                                               <  2:1095181/237‑1 (MQ=255)

                                                                                                                                                                     ggTGACAAAAAAAATAAACCCAC‑ATTTTTAGTGG‑TAGTGTGTGGCGG‑T‑GAATTTTTCCAGAAAAATAAAAAAACGGGA‑GTGGCCA‑TTAGAGGCAAAAGTAATAAGC‑AACAGGCAGTAATTAAGTTAAAAGAGATTGTCGTT‑AACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGAT‑GCCGAAACGTCGTCGATGGGGAAAATTAt                                                             <  1:506847/239‑1 (MQ=255)

                                                                                                                                                                      tgggCAAAAAATATAAACCCAC‑ATTTTTAGGGG‑TAGTTTGTGGCGG‑A‑GAATTTTTCAAGAAAAATAAAAAAACTGGA‑GTTGCCA‑TGATGAGCGAAAGTAATAAGC‑AACAGGCAGTAAATAAGTTGACAGAGATTGTCGCT‑AACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGAT‑GCCGAAACGTCGTCGATGGGGAAAATTAt                                                             <  2:190656/236‑1 (MQ=255)

                                                                                                                                                                      gtgaaaaaaaaTAAAAAACCAA‑ATTTGTAGTGG‑TAGGTTGTGGCGG‑T‑GAATTTTACCAAAAAAAAACAAAAAGTGGA‑GTTGCCA‑TGATGAGCGAAAGAAATAAGC‑AACAGGCAGTGAATAAGTGGACAGAGATTGTCGCT‑AACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGAT‑GCCGAAACGTCGTCGATGGGGAAAATTAt                                                             <  2:194187/233‑1 (MQ=255)

                                                                                                                                                                       tGGCAAAAATAATAAAACCAC‑ATTTTTATTGT‑TAGTTTGTGGCGG‑T‑AAATTTTTCCAGACAAATAAAAAAACGGGA‑GTTGCCT‑TGATGAGCAAAATAAATAAGC‑AACAGGCAGTAAATAAGTTGACAGAGATTGTCGCT‑AACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGAT‑GCCGAAACGTCGTCGATGGGGAAAATTATc                                                            <  1:1036074/238‑1 (MQ=255)

                                                                                                                                                                         gCAAAAAATATAAAACCAC‑ATTTTTAGTGG‑TAGTTTGTGGCGG‑T‑GAATTTTTTCAGACAAATAAAAAAACTGGA‑GTTGCCA‑TGATGAGCGGAAGGAATAAAC‑AACAAGCAGGGAAAAAGTTGACAGAGATTGTCGCT‑AACTTTAACGCCAAGATTTTTAACCGAATGCCTGATGACGTGGGGGGTAAAATAAAAAAGCTAAAGGAT‑GCCGAAAACTCGGCGATGGGGGAAAATATCTa                                                          >  1:608616/1‑238 (MQ=255)

                                                                                                                                                                         gCAAAAAATATAAAACCAC‑ATTTTTAGTGG‑TAGTTTGTGGCGG‑T‑GAATTTTTTCAAAAAAAAAAAAAAAATGGG‑GGTGGCA‑TGGTGAGCGGAAGGAAAAAAA‑AAAAGGCAGGGAATAAAATGAAAAAGAATGTTGCT‑AACATTAACCCCACGGTTTTTTCCCCAAATCCCGATGGCGCGGGGGGTAAAATCAAAAAACCAAAAAAT‑GCCGAAAAGAGGTCGCGGGGGGGAAATATCTa                                                          >  2:1048868/1‑238 (MQ=255)

                                                                                                                                                                             aaaaaaTAAAGCGAC‑ATTTTTAGTGG‑TATTTTGTGGCGG‑T‑TAATTTTTCAAAAAAAAAACAAAAACGGGA‑GTTGCCA‑TGATGACGGAAAGAAAAAAGC‑AACAGGCAGTGAATAATTTCACATAGATTGCCGCT‑AACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGAT‑GCCGAAACGTCGTCGATGGGGAAAATTATCTACCATa                                                     <  2:1199158/239‑1 (MQ=255)

                                                                                                                                                                             aaaaaaTAAAACCAC‑ATTTGTAGGGG‑TAGTTTGTGGCGT‑T‑AAATTTTTCCAGAAAAATACAAAAACGGGA‑GTTGCCA‑TGATGACGAAAAGTAATAAGC‑AACAGGCAGTGAATAAGTTGACAGAGTTTGTCGCT‑AATTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGAT‑GCCGAAACGTCGTCGATGGGGAAAATTATCTACCATa                                                     <  1:555187/239‑1 (MQ=255)

                                                                                                                                                                             aaaaTAAAAAACCAC‑ATTTTTAGGGG‑TAGTTTGTGGCGG‑T‑AAATTTTCCCAGAAAAAAACAAAAACGGGA‑GTTGCCA‑TGATGAGCGAAAGTAATAAGC‑AACAGGCAGTGAATAAGTTGACAGAGTTTGTCGCT‑AACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGAT‑GCCGAAACGTCGTCGATGGGGAAAATTATCTACCATa                                                     <  1:680575/239‑1 (MQ=255)

                                                                                                                                                                                aaaaaaaaACACAATTTTTAGTGG‑TATTTTGTGGGGT‑T‑AAATTTTTCAAAAAAAATAAAAAAACTGGA‑GTTGCCA‑TGAGGAGCAAAAGTAATAAGC‑AACAGGCAGTGAATAAGTGGACAGAGATGGTCGCT‑AACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGAT‑GCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACg                                                   <  2:262041/235‑1 (MQ=255)

                                                                                                                                                                                   taAAACCAC‑ATTTTTAGTGG‑TAGTTTGTGGCGG‑T‑GAATTTTTTCAGACAAATACAAAAAAAGGG‑GTTGCCA‑TGATGAGCGAAAGTAATAAAC‑AACAGGCAGTGAATAAGTTGACAGAGATTGTCGCT‑AACTTTACCGCCCTGATTTCTTCCCGAATGCCTGATGAc                                                                                                                         >  1:478258/1‑166 (MQ=255)

                                                                                                                                                                                         ccgaaTTTTTAGTGG‑TAGTTTGTGGCGG‑T‑AAATTTTTCAAGACAAATACAACAACTGGA‑GTTGCCA‑TGATGAGCAAAAGTAATAGGC‑ACAAGGCAGTGAATAATGTGACAGAAATGTCCGTT‑AACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGAT‑GCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGac                                          <  1:1177898/235‑1 (MQ=255)

                                                                                                                                                                                         cac‑aTTTTTAGTGG‑TAGTTTGTGGCGG‑T‑GAATTTTTCCAGACAAATACAAAAACTGGA‑GTTGCCA‑TGATGAGCGAAAGTAATAAGC‑AACAgg                                                                                                                                                                                              <  2:725546/92‑1 (MQ=255)

                                                                                                                                                                                          ac‑aTTTTAAGTGG‑TAGTTTGGGGCGG‑A‑AAAGTTGTCAAGAAAAATAAAAAAACGGGA‑GGTGCAA‑TGATGAGC‑AAAGAAAAAAACAAAGAGGTAGAAAAGAAGGTGACCAGGATAGTCGCT‑AACTTAACCGCCATGATTTCTACCCGAATGCCTGATGGCGTGGTGGATAAACTAAAACAGCTAAAGGAT‑GCCGAAACGTCGTCGATGGGGAAAATTATCTACCAGACGATGTTCGacaa                                        <  2:73837/239‑1 (MQ=255)

                                                                                                                                                                                                       g‑TAGTTTGTGGCGG‑T‑GAATTTTTTCCGACAAATACAAAAAATGGA‑GTTTCCC‑TGATTAGCGGAAGTAATAAAC‑AACAAGCAGTGAATAAGTTGGCAGAGATTTTCGCT‑AACTTTACCGCCCTGATTTTTTCCCCAATGCCTGATGACGTGGTGGATAAACTAAAAAAACTAAAGGAT‑GCCGCAACGTCGTCGATGGGGGAAATTATCTACCATACGATGTTCGACAACATGCcaaaaac                            >  1:76032/1‑237 (MQ=255)

                                                                                                                                                                                                          attttGTGGCGG‑T‑GAATTTTCCAAGACAAAAAAAAAACCTGGA‑TTTGCAA‑GGATGAGGGAAAGTAATAAGC‑AACAGGCAGTGAATAAGTGGACAGAGATTGTCCCT‑AACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGAT‑GCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGa                          <  1:898436/237‑1 (MQ=255)

                                                                                                                                                                                                                tgGGGG‑T‑AAATTTATCAAGAAAAAAAAAAAAACGGGG‑GATGCAA‑TGATGAGAAAAAGTAATAAGC‑AAAAGGCAGTGAATAAGTTAGAGGGGATTGCCGTA‑AACTTGACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGAT‑GCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGAc                     <  2:339576/238‑1 (MQ=255)

                                                                                                                                                                                                                           aattttCCAAAAAAAAAAAAAAACTGGG‑GTTGGCATTAAGGGGCGAAAGAAAAAAGC‑AAAGGGCAGTGAAAAAGTGGAAGGGGATTGTCGCT‑AACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGAT‑GCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCg             <  2:987055/236‑1 (MQ=255)

                                                                                                                                                                                                                                acaaGACAAATAAAAAAACTGGT‑GTTGCCA‑TTATGAGCAAAAGTAATAAGC‑ACCAGGCGGTGAATAAGTTGACAGAGATTGTCGCT‑AACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGAT‑GCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTcctgcc      <  1:1231645/236‑1 (MQ=255)

                                                                                                                                                                                                                                    gACAAATACAAAAACTGGA‑GTTGCCA‑TGATGAGCGAAAGTAATAAGC‑AACAGGCAGTGAATAAGTTGACAGAGATTGTCGCT‑AACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGAT‑GCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTcctgcatggc  >  2:939611/1‑237 (MQ=255)

CCAGTACCTGCAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTAGTGG‑TAGTTTGTGGCGG‑T‑GAATTTTTCCAGACAAATACAAAAACTGGA‑GTTGCCA‑TGATGAGCGAAAGTAATAAGC‑AACAGGCAGTGAATAAGTTGACAGAGATTGTCGCT‑AACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGAT‑GCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTC  >  NC_000913/3206214‑3206676

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`