Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,145,076 A→C 14.4% N361T (AAC→ACC)  pflD → putative glycine radical domain‑containing pyruvate formate‑lyase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,145,0760AC14.4% 340.6 / 3.9 162N361T (AAC→ACC) pflDputative glycine radical domain‑containing pyruvate formate‑lyase
Reads supporting (aligned to +/- strand):  ref base A (81/44);  new base C (2/31);  total (83/79)
Fisher's exact test for biased strand distribution p-value = 3.65e-10
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 165 total aligned reads displayed.

TCATTAACCCAGGGCGAAGATGCGGCGTTCCTGAAAGAACTGCTCGAATCTTTATGGGTGAAATGCAACGACATCGTGCTGTTGCGCTCCACCAGTAGCGCGCGTTATTTCGCCGGTTTCCCGACCGGCTATACCGCACTGCTCGGCGGGTTAACCGAGAACGGACGTAGCGCGGTGAACGTGCTTTCGTTCCTTTGCCTTGACGCCTATCAAAGCGTGCAATTACCGCAACCGAACCTCGGCGTGCGCACTAACGCCTTGATCGACACGCCGTTCCTGATGAAAACCGCCGAAACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCGTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCT  >  NC_000913/4144841‑4145313
                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                              
gcggtgaCCCAGGGCGAAGATGCGGCGTTCCTGAAAGAACTGCTCGAATCTTTATGGGTGAAATGCAACGACATCGTGCTGTTGCGCTCCACCAGTAGCGCGCGTTATTTCGCCGGTTTCCCGACCGGCTATACCGCACTGCTCGGCGGGTTAACCGAGAACGGACGTAGCGCGGTGAACGTGCTTTCGTTCCTTTGCCTTGACGCCTATCAAAGCGTGCAATTACCGCAACCGAAcc                                                                                                                                                                                                                                              <  1:1417232/232‑1 (MQ=255)
 catTAACCCAGGGCGAAGATGCGGCGTTCCTGAAAGAACTGCTCGAATCTTTATGGGTGAAATGCAACGACATCGTGCTGTTGCGCTCCACCAGTAGCGCGCGTTATTTCGCCGGTTTCCCGACCGGCTATACCGCACTGCTCGGCGGGTTAACCGAGAACGGACGTAGCGCGGTGAACGTGCTTTCGTTCCTTTGCCTTGACGCCTATCCAAGCGTGCAATTACCGCAACCGAACCt                                                                                                                                                                                                                                             >  1:299653/1‑238 (MQ=255)
 catTAACCCAGGGCGAAGATGCGGCGTTCCTGAAAGAACTGCTCGAATCTTTATGGGTGAAATGCAACGACATCGTGCTGTTGCGCTCCACCAGTAGCGCGCGTTATTTCGCCGGTTTCCCGACCGGCTATACCGCACTGCTCGGCGGGTTAACCGAGAACGGACGTAGCGCGGTGAACGTGCTTTCGTTCCTTTGCCTTGACGCCTATCAAAGCGTGCAATTACCGCAACCGAACCTc                                                                                                                                                                                                                                            >  1:111661/1‑239 (MQ=255)
  atTAACCCAGGGCGAAGATGCGGCGTTCCTGAAAGAACTGCTCGAATCTTTATGGGTGAAATGCAACGACATCGTGCTGTTGCGCTCCACCAGTAGCGCGCGTTATTTCGCCGGTTTCCCGACCGGCTATACCGCACTGCTCGGCGGGTTAACCGAGAACGGACGTAGCGCGGTGAACGTGCTTTCGTTCCTTTGCCTTGACGCCTATCAAAGCGTGCAATTACCGCAACCGAACCTCg                                                                                                                                                                                                                                           <  2:1294788/239‑1 (MQ=255)
   ttAACCCAGGGCGAAGATGCGGCGTTCCTGAAAGAACTGCTCGAATCTTTATGGGTGAAATGCAACGACATCGTGCTGTTGCGCTCCACCAGTAGCGCGCGTTATTTCGCCGGTTTCCCGACCGGCTATACCGCACTGCTCGGCGGGTTAACCGAGAACGGACGTAGCGCGGTGAACGGGCTTTCGTTCCTTTGCCTTGACGCCTATCAAAGCGTGCAATTACCGCAACCGAACCTCg                                                                                                                                                                                                                                           >  2:1224472/1‑238 (MQ=255)
     aaCCCAGGGCGAAGATGCGGCGTTCCTGAAAGAACTGCTCGAATCTTTATGGGTGAAATGCAACGACATCGTGCTGTTGCGCTCCACCAGTAGCGCGCGTTATTTCGCCGGTTTCCCGACCGGCTATACCGCACTGCTCGGCGGGTTAACCGAGAACGGACGTAGCGCGGTGAACGTGCTTTCGTTCCTTTGCCTTGACGCCTATCAAAGCGTGCAATTACCGCAACCGAACCTCGGCg                                                                                                                                                                                                                                        >  1:67600/1‑239 (MQ=255)
      aCCCAGGGCGAAGATGCGGCGTTCCTGAAAGAACTGCTCGAATCTTTATGGGTGAAATGCAACGACATCGTGCTGTTGCGCTCCACCAGTAGCGCGCGTTATTTCGCCGGTTTCCCGACCGGCTATACCGCACTGCTCGGCGGGTTAACCGAGAACGGACGTAGCGCGGTGAACGTGCTTTCGTTCCTTTGCCTTGACGCCTATCAAAGCGTGCAATTACCGCCACCGAAACTCCGCGt                                                                                                                                                                                                                                       >  2:487207/1‑239 (MQ=255)
       cccAGGGCGAAGATGCGGCGTTCCTGAAAGAACTGCTCGAATCTTTATGGGTGAAATGCAACGACATCGTGCTGTTGCGCTCCACCAGTAGCGCGCGTTATTTCGCCGGTTTCCCGACCGGCTATACCGCACTGCTCGGCGGGTTAACCGAGAACGGACGTAGCGCGGTGAACGTGCTTTCGTTCCTTTGCCTTGACGCCTATCAAAGCGTGCAATTACCGCAACCGAACCTCGGCGTg                                                                                                                                                                                                                                      <  2:862902/239‑1 (MQ=255)
         ccaggCGAAGATGAGGCGTTCCTGAAAGAACTGCTCGAATCTTTATGGGTGAAATGCAACGACATCGTGCTGTTGCTCTCCACCAGTAGCGCGCGTTATTCCGCCGGTTTCCCGACCGGCTATACCGCACTGCGCGGCGGGTTAACCGAGAACGGACGTAGCGCGGTGAACGTGCTTTCGTTCCTTTGCCTTGACGCCTATCAAAGCGTGCAATTACCGCAACCGAACCTCGGCGTgcg                                                                                                                                                                                                                                    <  2:1166260/236‑1 (MQ=255)
           gggCGAAGATGCGGCGTTCCTGAAAGAACTGCTCGAATCTTTATGGGTGAAATGCAACGACATCGTGCTGTTGCGCTCCACCAGTAGCGCGCGTTATTTCGCCGGTTTCCCGACCGGCTATACCGCACTGCTCGGCGGGTTAACCGAGAACGGACGTAGCGCGGTGAACGTGCTTTCGTTCCTTTGCCTTGACGCCTATCAAAGCGTGCAATTACCGCAACCGGACCTCGGCGTgcgc                                                                                                                                                                                                                                   >  1:1261390/1‑238 (MQ=255)
           gggCGAAGATGCGGCGTTCCTGAAAGAACTGCTCGAATCTTTATGGGTGAAATGCAACGACATCGTGCTGTTGCGCTCCACCAGTAGCGCGCGTTATTTCGCCGGTTTCCCGACCGGCTATACCGCACTGCTCGGCGGGTTAACCGAGAACGGACGTAGCGCGGTGAACGTGCTTTCGTTCCTTTGCCTTGACGCCTATCAAAGCGTGCAATTACCGCAACCGAACCTCGGCGTgcgc                                                                                                                                                                                                                                   >  1:1188930/1‑238 (MQ=255)
           gggCGAAGATGCGGCGTTCCTGAAAGAACTGCTCGAATCTTTATGGGTGAAATGCAACGACATCGTGCTGTTGCGCTCCACCAGTAGCGCGCGTTATTTCGCCGGTTTCCCGACCGGCTATACCGCACTGCTCGGCGGGTTAACCGAGAACGGACGTAGCGCGGTGAACGTGCTTTCGTTCCTTTGCCTTGACGCCTATCAAAGCGTGCAATTACCGCAACCGAACCTCGGCGTgcgc                                                                                                                                                                                                                                   >  1:422272/1‑238 (MQ=255)
           gggCGAAGATGCGGCGTTCCTGAAAGAACTGCTCGAATCTTTATGGGTGAAATGCAACGACATCGTGCTGTTGCGCTCCACCAGTAGCGCGCGTTATTTCGCCGGTTTCCCGACCGGCTATACCGCACTGCTCGGCGGGTTAACCGAGAACGGACGTAGCGCGGTGAACGTGCTTTCGTTCCTTTGCCTTGACGCCTATCAAAGCGGGCAATTACCGCAACCGACCCTCGGCGtcccc                                                                                                                                                                                                                                   >  2:281961/1‑234 (MQ=255)
           gggCGAAGATGCGGCGTTCCTGAAAGAACTGCTCGAATCTTTATGGGTGAAATGCAACGACATCGTGCTGTTGCGCTCCACCAGTAGCGCGCGTTATTTCGCCGGTTTCCCGACCGGCTATACCGCACTGCTCGGCGGGTTAACCGAGAACGGACGTAGCGCGGGGAACGTGCTTTCGTTCCTTTGCCTTGACGCCCATCCAAGCGTGCAATTACCCCAACCGAACCTCCGctttggc                                                                                                                                                                                                                                   >  2:1329322/1‑232 (MQ=255)
           gggCGAAGATGCGGCGTTCCTGAAAGAACTGCTCGAATCTTTATGGGTGAAATGCAACGACATCGTGCTGTTGCGCTCCACCAGTAGCGCGCGTTATTTCGCCGGTTTCCCGACCGGCTATACCGCACTGCTCGGCGGGTTAACCGAGAACGGACGCAGCGCGGTGAACGGGCTTTCGTTCCTTTTCCTTGACGCCTATCAAAGCGTGCAATTACCGCAACCGCACCTCGGCGTgcgc                                                                                                                                                                                                                                   >  1:591420/1‑238 (MQ=255)
           gggCGAAGATGCGGCGTTCCTGAAAGAACTGCTCGAATCTTTATGGGTGAAATGCAACGACATCGTGCTGTTGCGCTCCACCAGTAGCGCGCGTTATTTCGCCGGTTTCCCGACCGGCTATACCGCACTGCTCGGCGGGTTAACCGAGAACGGACCTAGCGCGGTGAACGGGCTTTCGTTCCCTTGCCTTGACGCCTATCAAAGCGTGCAATTACCGCAACCGTACCTCGGCGTgcgc                                                                                                                                                                                                                                   >  1:335447/1‑238 (MQ=255)
           gggCGAAGATGCGGCGTGCCTGAAAGAACTGCTCGAATCTTTATGGGTGAAATGCAACGACATCGTGCTGTTGCGCTCCACCAGTAGCGCGCGTTATTTCGCCGGTTTCCCGACCGGCTATACCGCACTGCTCGGCGGGTTAACCGAGAACGGACGTAGCGCGGTGAACGTGCTTTCGTTCCTTTGCCTTGACGCCTATCAAAGCGTGCAATTACCGCAACCGAACCTc                                                                                                                                                                                                                                            <  2:165593/229‑1 (MQ=255)
            ggCGAAGATGCGGCGTTCCTGAAAGAACTGCTCGAATCTTTATGGGTGAAATGCAACGACATCGTGCTGTTGCGCTCCACCAGTAGCGCGCGTTATTTCGCCGGTTTCCCGACCGGCTATACCGCACTGCTCGGCGGGTTAACCGAGAACGGACGTAGCGCGGTGAACGTGCTTTCGTTCCTTTGCCTTGACGCCTATCAAAGCGTGCAATTACCGCAACCGAACCTCGGCGTgcgc                                                                                                                                                                                                                                   >  1:973978/1‑237 (MQ=255)
            ggCGAAGATGCGGCGTTCCTGAAAGAACTGCTCGAATCTTTATGGGTGAAATGCAACGACATCGTGCTGTTGCGCTCCACCAGTAGCGCGCGTTATTTCGCCGGTTTCCCGACCGGCTATACCGCACTGCTCGGCGGGTTAACCGAGAACGGACGTAGCGCGGGGAACGTGCTTTCGTTCCTTTGCCTTGACGCCTATCAAAGCGTGCAATTACCGCCACCGACCCTCGGCGTgcgcc                                                                                                                                                                                                                                  >  2:1257701/1‑237 (MQ=255)
              cGAAGATGCGGCGTTCCTGAAAGAACTGCTCGAATCTTTATGGGTGAAATGCAACGACATCGTGCTGTTGCGCTCCACCAGTAGCGCGCGTTATTTCGCCGGTTTCCCGACCGGCTATACCGCACTGCTCGGCGGGTTAACCGAGAACGGACGTAGCGCGGTGAACGTGCTTTCGTTCCTTTGCCTTGACGCCTATCAAAGCGTGCAATTACCGCAACCGAACCTCGGCGTGCGCACt                                                                                                                                                                                                                                <  2:673505/238‑1 (MQ=255)
              cGAAGAGGAGGCGTGACTGGAAGAACTGATAGAATCGTTATGGTTGAAATGAAACGACACCGTGCTGTTGCGCACCACCAGTAGCGCGCGTTATTTCGCCGGGTTCCCGACCGGGTAGACCGCACTGCTCGGCGGGTTAACCGAGAACGCACGTAGCGCGGTGAACGTGCTTTCGTTCCTTTGCCTTGACGCCGATCAAAGCGTGCAATTACCGCAGCCGAACCTCGGCGTGCGAACt                                                                                                                                                                                                                                <  2:484040/238‑1 (MQ=255)
               gAAGATGCGGCGTTCCTGAAAGAACTGCTCGAATCTTTATGGGTGAAATGCAACGACATCGTGCTGTTGCGCTCCACCAGTAGCGCGCGTTATTTCGCCGGTTTCCCGACCGGCTATACCGCACTGCTCGGCGGGTTAACCGAGAACGGACGTAGCGCGGTGAACGTGCTTTCGTTCCTTTGCCTTGACGCCTATCAAAGCGTGCAATTACCGCAACCGAACCTCGGCGTGCGCACTa                                                                                                                                                                                                                               <  2:806090/238‑1 (MQ=255)
               gAAGATGCGGCGTTCCTGAAAGAACTGCTCGAATCTTTATGGGTGAAATGCAACGACATCGTGCTGTTGCGCTCCACCAGTAGCGCGCGTTATTTCGCCGGTTTCCCGACCGGCTATACCGCACTGCTCGGCGGGTTAACCGAGAACGGACGTAGCGCGGTGAACGTGCTTTCGTTCCTTTGCCTTGACGCCTATCAAAGCGTGCAATTACCGCAACCGAACCTCGGCGTGCGCACTa                                                                                                                                                                                                                               <  1:399840/238‑1 (MQ=255)
                    tGCGGCGTTCGTGAAAGAACTTCTCGAAGCTTTATGGGTGAAATGCAACGACATCGTGCTGTTGCGCTCCAACAGTAGCGCGCGTTATTTCGCCGGTTTCCCGACCGGCTATACCGCACTGCTCGGCGGGTTAACCGAGAACGGACGTAGCGCGGTGAACGTGCTTTCGTTCCTTTGCCTTGACGCCTATCAAAGCGTGCAATTACCGCAACCGAACCTCGGCGTGCGCACTAACGcc                                                                                                                                                                                                                          <  2:1123941/238‑1 (MQ=255)
                    agcggcgTCGTTGAAAGACCTGCGCGAATCTTTATGGGTGAAATGCAGCGGCCGCGTGCTTTGTGGCTCCACCGGTGGCGCGCATGAATCCGCCGGTTTCCCGACCGGCTATACCGCACTGCTCGGCGGGTTAAACGAGAACGGACGTATCGCTGTGAACGTGCTTTCGTTCCTTTGCCGTGTCGCCTATCATAGCGTGCAATTTCCGCAACCGAGCCTCGGCGTGCGCACTAACGc                                                                                                                                                                                                                           <  2:1344514/236‑1 (MQ=255)
                      cggcgTTCCTGAAAGAACTGCTCGAATCTTTATGGGTGAAATGCAACGACATCGTGCTGTTGCGCTCCACCAGTAGCGCGCGTTATTTCGCCGGTTTCCCGACCGGCTATACCGCACTGCTCGGCGGGTTAACCGAGAACGGACGTAGCGCGGTGAACGTGCTTTCGTTCCTTTGCCTTGACGCCTATCAAAGCGTGCAATTACCGCAACCGAACCTCGGCGTg                                                                                                                                                                                                                                      >  1:504219/1‑224 (MQ=255)
                      cggcgTTCCTGAAAGAACTGCTCGAATCTTTATGGGTGAAATGCAACGACATCGTGCTGTTGCGCTCCACCAGTAGCGCGCGTTATTTCGCCGGTTTCCCGACCGGCTATACCGCACTGCTCGGCGGGTTAACCGAGAACGGACGTAGCGCGGTGAACGTGCTTTCGTTCCTTTGCCTTGACGCCTATCAAAGCGTGCAATTACCGCAACCGAACCTCGGCGTGCGCCCTAACGCCTTg                                                                                                                                                                                                                       >  2:359801/1‑239 (MQ=255)
                      cggcgTTCCTGAAAGAACTGCTCGAATCTTTATGGGTGAAATGCAACGACATCGTGCTGTTGCGCTCCACCAGTAGCGCGCGTTATTTCGCCGGTTTCCCGACCGGCTATACCGCACTGCTCGGCGGGTTAACCGAGAACGGACGTAGCGCGGTGAACGTGCTTTCGTTCCTTTGCCTTGACGCCTATCAAAGCGTGCAATTACCGCAACCGAACCTCGGCGTGCGCACTAACGCCTTg                                                                                                                                                                                                                       >  1:233768/1‑239 (MQ=255)
                            tCCTGAAAGAACTGCTCGAATCTTTATGGGTGAAATGCAACGACATCGTGCTGTTGCGCTCCACCAGTAGCGCGCGTTATTTCGCCGGTTTCCCGACCGGCTATACCGCACTGCTCGGCGGGTTAACCGAGAACGGACGTAGCGCGGTGAACGTGCTTTCGTTCCTTTGCCTTGACGCCTATCAAAGCGTGCAATTACCGCAACCGAACCTCGGCGTGCGCACTAACGCCTTGATCGac                                                                                                                                                                                                                <  1:926146/239‑1 (MQ=255)
                                      aCTGCTCGAAGCTTTATGGGGCAAATGCACCGCCATCGTTCTGTTGCGTTCCTCCAGTAGCGCGCGTTATTTCGCCGTTTTCCCGGCCGGCTATACCGCGCTGCTCGGCGGGTTAACCGAGAACGGACGTAGCGCGGTGAACGTGCTTTCGTTCCTTTGCCTTGACGCCTATCAAAGCGTGCAATTTCCGCACCCGAGCCTCGGCGTGCGCACTAACGCCTTGATCGACACGCCGTTc                                                                                                                                                                                                       <  2:834806/238‑1 (MQ=255)
                                        tGCTCGAATCTTTATGGGTGAAATGCAACGACATCGTGCTGTTGCGCTCCACCAGTAGCGCGCGTTATTTCGCCGGTTTCCCGACCGGCTATACCGCACTGCTCGGCGGGTTAACCGAGAACGGACGTAGCGCGGTGAACGTGCTTTCGTTCCTTTGCCTTGACGCCTATCAAAGCGTGCAATTACCGCAACCGAACCTCGGCGTGCGCACTAACGCCTTGATCGACACGCCGTTCCtg                                                                                                                                                                                                    >  1:107806/1‑239 (MQ=255)
                                                           gAAATGCAACGACATCGTGCTGTTGCGCTCCACCAGTAGCGCGCGTTATTTCGCCGGTTTCCCGACCGGCTATACCGCACTGCTCGGCGGGTTAACCGAGAACGGACGTAGCGCGGTGAACGGGCTTTCGTTCCTTTGCCTTGACGCCTATCAAAGCGTGCAATTACCGCAACCGAACCTCGGCTTGCGCACTAACGCCTTGATCGACACGCCGTTCCTGATGAAAACCGCCGAAACCa                                                                                                                                                                                 >  1:804453/1‑239 (MQ=255)
                                                            aaaTGCAACGACATCGTGCTGTTGCGCTCCACCAGTAGCGCGCGTTATTTCGCCGGTTTCCCGACCGGCTATACCGCACTGCTCGGCGGGTTAACCGAGAACGGACGTAGCGCGGTGAACGTGCTTTCGTTCCTTTGCCTTGACGCCTATCAAAGCGTGCAATTACCGCAACCGAACCTCGGCGTGCGCACTAACGCCTTGATCGACACGCCGTTCCTGATGAACACCGCCGAAAACAt                                                                                                                                                                                >  2:193264/1‑239 (MQ=255)
                                                            aaaTGCAACGACATCGTGCTGTTGCGCTCCACCAGTAGCGCGCGTTATTTCGCCGGTTTCCCGACCGGCTATACCGCACTGCTCGGCGGGTTAACCGAGAACGGACGTAGCGCGGTGAACGTGCTTTCGTTCCTTTGCCTTGACGCCTATCAAAGCGTGCAATTACCGCAACCGAACCTCGGCGTGCGCACTAACGCCTTGATCGACACGCCGTTCCTGATGAAAACCGCAGAAACCAt                                                                                                                                                                                >  2:842044/1‑239 (MQ=255)
                                                                   aCGACATCGTGCTGTTGCGCTCCACCAGTAGCGCGCGTTATTTCGCCGGTTTCCCGACCGGCTATACCGCACTGCTCGGCGGGTTAACCGAGAACGGACGTAGCGCGGTGAACGTGCTTTCGTTCCTTTGCCTTGACGCCTATCAAAGCGTGCAATTACCGCAACCGAACCTCGGCGTGCGCACTAACGCCTTGATCGACACGCCGTTCCTGATGAAAACCGCCGAAACCATTCGCTTc                                                                                                                                                                         >  1:1122553/1‑239 (MQ=255)
                                                                           gTGCTGTTGCGCTCCACCAGTAGCGCGCGTTATTTCGCCGGTTTCCCGACCGGCTATACCGCACTGCTCGGCGGGTTAACCGGGAACGGGCGGAGCGCGGTGGACGTGCTTTCGTTCCTTTGCCCTGACGGCTATCAAAGCGTGCAATTACCGCAAACCAAAATCGCCGCGCGCAATAATGCCTTGTTCGACCCGCCGTTCCTGATGAAAACCGCCCAAAACATTCTCTTCGGTACCgg                                                                                                                                                                 >  2:1440725/1‑239 (MQ=255)
                                                                                      cTCCACCAGTAGCGCGCGTTATTTCGCCGGTTTCCCGACCGGCTATACCGCACTGCTCGGCGGGTTAACCGAGAACGGACGTAGCGCGGTGAACGTGCTTTCGTTCCTTTGCCTTGACGCCTATCAAAGCGTGCAATTACCGCAACCGAACCTCGGCGTGCGCACTAACGCCTTGATCGACACGCCGTTCCTGATGAAAACCGCCGAAACCATTCGCTTCGGTACCGGTATTCCGCcaa                                                                                                                                                      >  2:1377702/1‑239 (MQ=255)
                                                                                      cTCCACCAGTAGCGCGCGTTATTTCGCCGGTTTCCCGACCGGCTATACCGCACTGCTCGGCGGGTTAACCGAGAACGGACGTAGCGCGGTGAACGTGCTTTCGTTCCTTTGCCTTGACGCCTATCAAAGCGTGCAATTACCGCAACCGAACCTCGGCGTGCGCACTAACGCCTTGATCGACACGCCGTTCCTGATGAAAACCGCCGAAACCATTCGCTTCGGTACCGGTATTCCGCaaa                                                                                                                                                      >  1:1251762/1‑239 (MQ=255)
                                                                                                   gcgcgTTATTTCGCCGGTTTCCCGACCGGCTATACCGCACTGCTCGGCGGGTTAACCGAGAACGGACGTAGCGCGGTGAACGTGCTTTCGTTCCTTTGCCTTGACGCCTATCAAAGCGTGCAATTACCGCAACCGAACCTCGGCGTGCGCACTAACGCCTTGATTGACACGCCCGTCCTGATGACAACCCCCGCAACCATTCGCTTCGGTACCGGTATTCCGCAAATCCTTGACGATGa                                                                                                                                         >  2:742818/1‑239 (MQ=255)
                                                                                                   gcgcgTTATTTCGCCGGTTTCCCGACCGGCTATACCGCACTGCTCGGCGGGTTAACCGAGAACGGACGTAGCGCGGTGAACGTGCTTTCGTTCCTTTGCCTTGACGCCTATCAAAGCGTGCAATTACCGCAACCGAACCTCGGCGTGCGCACTAACGCCTTGATCGACACGCCGTTCCTGATGAAAACCGCCGAAACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGa                                                                                                                                         >  1:543794/1‑239 (MQ=255)
                                                                                                   gcgcgTTATTTCGCCGGTTTCCCGACCGGCTATACCGCACTGCTCGGCGGGTTAACCGAGAAAGGACGTAGCGCGGTGAACGTGCTTTCCTTCCTTTGCCTTGACGCCTATCAAAGCGTGCAATTACCGCAACCGAACCTCGGCGTGCGCACTTACGCCTTGGTCGACACGCCCGTCCTTATGAAAACCCCCGAAAACATCCGCCTCGGTACCCGGATTACGCAAATCTTTTACCAtta                                                                                                                                         >  2:431140/1‑237 (MQ=255)
                                                                                                               gCCGGTTTCCCGACCGGCTATACCGCACTGCTCGGCGGGTTAACCGAGAACGGACGTAGCGCGGTGAACGTGCTTTCGTTCCTTTGCCTTGACGCCTATCAAAGCGTGCAATTACCGCAACCGAACCTCGGCGTGCGCACTAACGCCTTGATCG‑‑ACGCCGTTCCTGATGAAAACCGCCGAAACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAg                                                                                                                           <  1:1331288/239‑1 (MQ=255)
                                                                                                                 cGGTTCCCCCACCGGTTATGCCGCGCTCTCCGGCGGGTTAACCGAGAACGGACGTAGCGCGGTGAACGTTCTTTCGTTCCTTTGCCTTGACGCCTATCAAAGCGTGCAATTACCGCACCCGACCCTCGGCTTGCCCCCAACCGCCTTGACCGACACGCCGTTCCTGATGAAAACCGCCGAAACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAg                                                                                                                           <  2:693690/239‑1 (MQ=255)
                                                                                                                              ggCTATACCGCACTGCTCGGCGGGTTAACCGAGAACGGACGTAGCGCGGTGAACGTGCTTTCGTTCCTTTGACTTGACGCCTATCAAAGCGTGCAATTACCGCAACCGAACCTCGGCGTGCGCACTAACGCCTTGATCGACACGCCGTTCCTGATGAAAACCGCCGAAACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCg                                                                                                              >  1:1149173/1‑239 (MQ=255)
                                                                                                                                   taCCGCCCTGCGCGGCGGTTTAACCGAAAACTGCCGTGGCGGGGTGAACGTGCTTCCTTTCTTTTGCCTTGCCCCCAATCAAAGCGGGCAAATCCCGCAACCGACCCTCGGCGTGCGCACTAACCCCTTGTTCGCCACGCCGTTCCTGATGAAAACCGCCGAAACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCg                                                                                                         <  2:107806/239‑1 (MQ=255)
                                                                                                                                       gCACTGCTCGGCGGGTTAACCGAGAACGGACGTAGCGCGGTGAACCTGCTTTCGTTCCTTTGCCTTGACGCCTATCAAAGCGTGCAATTACCGCAACCGCACCTCGGCGTGCGCACTAACGCCTTGATCGACACCCCGTTCCTGATGCAAACCTCCGAAACCATTCGCTTCGGCACAGGTATTCCGCAAACCTTTAACGTTCAAGGGGTGGTGCCAGCGGTCCTCAACCGTGGGGTTTc                                                                                                     >  2:565956/1‑239 (MQ=255)
                                                                                                                                          cTGCTCGGCGGGTTAACCGAGATGTGGCGTATCGCGGTGAACGTGCCTTCGTTCCTTTGCCTTGACGCCTATCAAAACGAGCAATTACCGCAACCGAACCTCGGCGGGCGCACTCACGCCTTGATCGACACGCCGTTCCTGATGGAAACCGCCGAAACCATTCCCCTCGGCACCGGACTTCCGCTAATCTTTAACCATTAAGTGGGGGTGCCCCCGTTACCCAACCCAGGGGtttg                                                                                                     >  2:1111528/1‑235 (MQ=255)
                                                                                                                                             cTCGGCGGGTTAACCGAGAACGGACGTAGCGCGGTGAACGTGCTTTCGTTCCTTTGCCTTGACGCCTATCAAAGCGTGCAATTACCGCAACCGAACCTCGGCGTGCGCACTAACGCCTTGATCGACACGCCGTTCCTGATGAAAACCGCCGAAACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGa                                                                                               >  1:1324231/1‑239 (MQ=255)
                                                                                                                                             cTCGGCGGGTTAACCGAGAACGGACGTAGCGCGGTGAACGTGCTTTCGTTCCTTTGCCTTGACGCCTATCAAAGCGTGCAATTACCGCAACCGAACCTCGGCGTGCGCACTAACGCCTTGATCGACACGCCGTTCCTGATGAAAACCGCCGAAACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGa                                                                                               >  1:1312852/1‑239 (MQ=255)
                                                                                                                                             cTCGGCGGGTTAACCGAGAACGGACGTAGCGCGGTGAACGTGCTTTCGTTCCTTTGCCTTGACGCCTATCAAAGCGTGCAATTACCGCAACCGAACCTCGGCGTGCGCACTAACGCCTTGATCGACACGCCGTTCCTGATGAAAACCGCCGAAACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGa                                                                                               >  1:744894/1‑239 (MQ=255)
                                                                                                                                             cTCGGCGGGTTAACCGAGAACGGACGTAGCGCGGTGAACGTGCTTTCGTTCCTTTGCCTTGACGCCTATCAAAGCGTGCAATTACCGCAACCGAACCTCGGCGTGCGCACTAACGCCTTGATCGACACGCCGTTCCTGATGAAAACCGCCGAAACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGa                                                                                               >  1:648985/1‑239 (MQ=255)
                                                                                                                                             cTCGGCGGGTTAACCGAGAACGGACGTAGCGCGGTGAACGTGCTTTCGTTCCTTTGCCTTGACGCCTATCAAAGCGTGCAATTACCGCAACCGAACCTCGGCGTGCGCACTAACGCCTTGATCGACACGCCGTTCCTGATGAAAACCGCCGAAACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGGGGGGTTTTGCTGGa                                                                                               >  2:1002160/1‑239 (MQ=255)
                                                                                                                                                   ggtttAACCAAGACCGGCCGTAGCGCGGTGAACGTGCTTTCGTTCCTTTGCTTTGACGCCTATAAAAGCGTGCAATTCCCGCACCCGACCCTCGGCGTGCGCACTAACGCCTTGATCGCCACGCCGTTCCTGATGAAAACCGCCGAAACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATg                                                                                          <  2:1251762/235‑1 (MQ=255)
                                                                                                                                                     gTTAACGGAGAACGGACGTGGCGCGGTGACCGTGCTTTCGTTCCTTTGCCTGGACGCCTATCAAAGCGTGAATTTACCGCAACCGACCCTCGGCGTGCGCACTACCGCCTTGATCGCCACGCCGTTCCTGATGAAAACCGCCGAAACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATgcg                                                                                        <  2:422272/238‑1 (MQ=255)
                                                                                                                                                     gTTAACCGAGAACGGACGTAGCGCGGTGAACGTGCTTTCGTTCCTTTGCCTTGACGCCTATCCAAGCGTGCAATTACCGCAACCGGACCTCGGCGTGCGCACTAACGCCTTGATCGACACGCCGTTCCTGATGAAAACCGCCGAAACCATTCCGTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCCGCTTTCCTCAACCGTGCCGTTTCGCTGGAAGATgcg                                                                                        >  2:167229/1‑238 (MQ=255)
                                                                                                                                                       tAACCGAGAACGGCCGTAGCGCGGTGAACGTGCTTTCGTTCCTTTGCCTTGCCGCCTATAAAAGCGTGCAATTCCCGCACCCGAACCTCGGCGTGCGCACTAACGCCTTGATCGCCACGCCGTTCCTGATGAAAACCGCCGAAACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATgcgcgc                                                                                     <  2:692408/239‑1 (MQ=255)
                                                                                                                                                       tAACCGAGAACGGACGTAGCGCGGTGAACGTGCTTTCGTTCCTTTGCCTTGACGCCTATCAAAGCGTGCAATTACCGCACCCGAACCTCGGCGTGCGCACTAACGCCTTGATCGACACGCCGTTCCTGATGAAAACCGCCGAAACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATgcgcgc                                                                                     <  1:623245/239‑1 (MQ=255)
                                                                                                                                                         cccGCGAACGGACGTAGGGGGGTGAACGGCATTTCGTTCTTTTGCCTGGCCGCCTATCAAAGCGTGCAATTCCCGCAACCGACCCCCGGCGGGCGCACAAACGCCTTGACGGCCGCGCCGTCCGTGATGAAAACCGCCGAAACCATGCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGGGa                                                                                   <  2:357567/238‑1 (MQ=255)
                                                                                                                                                         acggagaACGGAGGTGGGGTGGTGAACGCGCTTGGGTATGTATGCCTGGACTTCTATCAGAGCGTGAAATTGCCGCAGCCGAGCCGGGGCGGGCGCACTACCGCCGTGCTCAACACCCGGTTCAAGATGAAAACCGCCGAAACCAGTCGCTTCGGTACCGGTATACCGCAAATCTTTAACGATGAAGAGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGATGGAAGATGCGCGCGa                                                                                   <  2:1203478/236‑1 (MQ=255)
                                                                                                                                                         aCCGAGAACGGACGTAGCGCGGTGAACGTGCTTTCGTTCCTTTGCCTTGACGCCTATCAAAGCGTGCAATTCCCGCAACCGACCCTCGGCGTGCGCACTAACGCCTTGACCGACACGCCGTTCCTGATGAAAACCGCCGAAACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGa                                                                                   <  1:100698/239‑1 (MQ=255)
                                                                                                                                                            gagaACGGACGTAGCGCGGTGACCGTGCTTCCGTTCCTTTGCCTTGCCGCCTATCAAAGCGTGCAATTCCCGCACCCGACCCCCGGCGTGCGCACTAACGCCTTGATCGACACGCCGTTCCTGATGAAAACCGCCGAAACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTa                                                                                <  1:281961/239‑1 (MQ=255)
                                                                                                                                                              aaaCGGACGAAGCGCGGTGAACGTGCTTTCGTCCCTTTGCCTGGACGCCTATCAAAGCGTGCAATTACCGCAACCGAACCTCGGCGTGCGCACTAACGCCTTGATCGACACGCCGTTCCTGATGAAAACCGCCGAAACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTAtt                                                                              <  1:423701/238‑1 (MQ=255)
                                                                                                                                                               accgaccgaaGCGCGGTGAACGTGTTTTCGTCCCTTTGCCTGGACGCCTATCAAAGCGTCAAATTACCGCACCCGACCCCCGGCGTGCGCCCTAACGCCTTGATCGACACGCCGTTCCTGATGAAAACCGCCGAAACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTAtt                                                                              <  2:648985/229‑1 (MQ=255)
                                                                                                                                                                aCGGACGTAGCGCGGTGAACGTGTTTTCTTTCCTTTGCCTTGACCCCTATCAAGGCGTGCATTTACCGCAACCGACCCTCGGCGTGCCCATTAACGCCTGGATCGACGCGCCGTTCCTGATGAAAACCGCCGAAACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTAtt                                                                              <  1:457649/237‑1 (MQ=255)
                                                                                                                                                                aCGGACGTAGCGCGGTGAACGTGCTTTCGTTCCTTTGCCTTGACGCCTATCAAAGCGTGCAATTACCGCAACCGAACCTCGGCGTGCGCACTAACGCCTTGATCGACACGCCGTTCCTGATGAAAACCGCCGAAACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTAtt                                                                              <  1:367005/237‑1 (MQ=255)
                                                                                                                                                                    gcGTGGCGGGGTGCAGTTGCTTTCGTTCCTTTGCCTTGCCGCCTACAAAGGCGTGCAATTACCGCACCCGACCCGCGGCGTGCGCACTACCGCCTTGATCGACACGCCGTTCCTGATGAAAACCGCCGAAACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCGTa                                                                         <  2:233768/237‑1 (MQ=255)
                                                                                                                                                                    aCGTAGCGCGGTGAACGTGCTTTCGTTCCTTTGCCTTGACGCCTATCAAAGCGTGCAATTACCGCAACCGAACCTCGGCGTGCGCACTAACGCCTTGATCGACACGCCGTTCCTGATGAAAACCGCCGAAACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCGTAg                                                                        >  1:465986/1‑239 (MQ=255)
                                                                                                                                                                        aGCGCGTTGAACGTGCTTTCGTTCCTTGCCCTTGACGCCTACCAAAGCGTGCAATTACCGCAACCGACCCCCGGCGTGCGCACTAACGCTTTGACCGACACGCCGTTCCTGATGAAAACCGCCGAAACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCGTAGTggg                                                                    <  1:1391665/239‑1 (MQ=255)
                                                                                                                                                                        aGCGCGGTGAACGTGCTTTCGTCCCTTTGCCTTGGCGCCTATCAAAGCGTGCAATTCCCGCAACCGAACCTCGGCGTGCGCACTAACGCTTTGATCGACACGCCGTTCCTGATGAAAACCGCCGAAACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCGTAGTggg                                                                    <  1:1184934/239‑1 (MQ=255)
                                                                                                                                                                                gAACGTGCTTTCGGTCCTTTGCCTTGACGCCTATCAAAGCGTGCAATTACCGCAACCGAACCTCGGCGTGCGCACTAACGCCTTGATCGACACGCCGTTCCTGATGAAAACCGCCGACACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCGTAGTGGGCTGTGTgg                                                            >  2:1241885/1‑239 (MQ=255)
                                                                                                                                                                                  aCGTGCTTTCTTTCCTTTGCCTTGACGCCTAGCAGAGCGTCGAATTCCCGCAACCGACCCTCGGCGTGCGCACTAACGCCTTGATCGACACCCCGTTCCTGATGAAAACCGCCGAAACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCGTAGTGGGCTGTGTGGaa                                                          <  1:1280541/239‑1 (MQ=255)
                                                                                                                                                                                         ttCGTTCCTTTGCCTTGACGCCTATCAAAGCGTGCAATTACCGCAACCGAACCTCGGCGTGCGCACTAACGCCTTGATCGACACGCCGTTCCTGATGAAAACCGCCGAAACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCg                                                                           >  1:435495/1‑215 (MQ=255)
                                                                                                                                                                                           cGTTCCTGTGCCTTGACGCCTTCAAACGGGGGAAATTACCGCAACCGAACCTCGGGGTGGGCACAAACGCCTTGACCGCACCGCCGTCCCTGATGAAAACCGCCGAAACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCGTAGTGGGCTGTGTGGAATTATCTAtt                                                 <  2:756511/239‑1 (MQ=255)
                                                                                                                                                                                                  ttGCCTTTACGCCTATCAAAGCGTCAAATTACCGCAACCGACCCTCGGCGTGCCCACTACCGCCTTGATCGCCACGCCGTTCCTGATGAAAACCGCCGAAACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCGTAGTGGGCTGTGTGGAATTATCTATTCCCGGCa                                          <  1:18481/239‑1 (MQ=255)
                                                                                                                                                                                                  ttGCCTTGACGCCTATCAAAGCGTGCAATTACCGCAACCGAACCTCGGCGTGCCCACTAGCGCCTGGCTCGCCACGCCGTTCCGGGTGAAAACCGCCGAAACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCGTAGTGGGCTGTGTGGAATTATCTATTCCCGGCa                                          <  1:567361/239‑1 (MQ=255)
                                                                                                                                                                                                  ttGCCGTGACGCCTATCAAACCGTGCAATTACCGCAACCGACCCCCGGCGTGCGCACTAACGCCTTGATCGACACGCCGTTCCTGATGAAAACCGCCGAAACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCGTAGTGGGCTGTGTGGAATTATCTATTCCCGGCa                                          <  1:1059119/239‑1 (MQ=255)
                                                                                                                                                                                                  gtGCCTGGACGCATAACACAGCGGGAAAATCCCGCACCGGAACCCGCGCGCGCGCACAACCGTCTTGACCGACACGCCGTTCCTGATAAAAACCGCCGAAACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCGTAGTGGGCTGTGTGGAATTATCTATTCCCGGCa                                          <  2:649913/238‑1 (MQ=255)
                                                                                                                                                                                                          aCGCCTATCAAAGCGTGCAATTACCGCAACCGAACCTCGGCGTGCGCACTAACGCCTTGATCGACACGCCGTTCCTGATGAAAACCGCCGAAACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCaa                                                                                                                 >  2:214141/1‑160 (MQ=255)
                                                                                                                                                                                                          aCGCCTATCAAAGCGTGCAATTACCGCAACCGAACCTCGGCGTGCGCACTAACGCCTTGATCGACACGCCGTTCCTGATGAAAACCGCCGAAACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCaa                                                                                                                 <  1:214141/160‑1 (MQ=255)
                                                                                                                                                                                                            cccttacaaacgCGTGTAATTCCTGCACCCGTTCCCCGGGTGGCGCACTACCCCCTGGTGCGAAGCGCCGTTCCTGATAAATACCGCCGAAACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCGTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACg                                 <  2:733209/228‑1 (MQ=255)
                                                                                                                                                                                                                       gCGTGCAATTACCGCAACCGAACCTCGGCGTGCGCACTAACGCCTTGATCGACACGCCGTTCCTGATGAAAACCGCCGAAACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCGTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGAc                      >  1:476044/1‑239 (MQ=255)
                                                                                                                                                                                                                          tGCAATTACCGCAACCGAACCTCGGCGTGCGCACTAACGCCTTGATCGACACGCCGTTCCTGATGAAAACCGCCGAAACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCGTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACAt                    >  1:369971/1‑238 (MQ=255)
                                                                                                                                                                                                                          tGCAATTACCGCAACCGAACCTCGGCGTGCGCACTAACGCCTTGATCGACACGCCGTTCCTGATGAAAACCGCCGAAACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCCCTGGAAGAGGCGCGCGCCTATTCCGTCGTGGGCTGTGTGGAATTTTCTGTTTCCGGCAGAACCTCCGGccgtgatgacat                    >  2:875506/1‑227 (MQ=255)
                                                                                                                                                                                                                            cAATTCCCGCAACCGACCCCCGGCGTGCGCACTAACGCCTTGATCGACACGCCGTTCCTGATGAAAACCGCCGAAACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCGTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATcg                  <  1:152845/238‑1 (MQ=255)
                                                                                                                                                                                                                            cAATTCCCGCAACCGAACCTCGGCGTGCGCACAAACGCCTTGTTCGACACGCCGTTCCTGAGAAAAACCGCCGAAACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCGTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATcgc                 <  2:525145/239‑1 (MQ=255)
                                                                                                                                                                                                                            cAATTACCGCACCCGACCCTCGGCGTGCGCCCTAACGCCTTGATCGACACGCCGTTCCTGATAAAAACCGCCGAAACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCGTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATcg                  <  2:369971/238‑1 (MQ=255)
                                                                                                                                                                                                                            cAATTACCGCAACCGAACCTCGGCGTGCGCACTAACGCCTTGATCGACACGCCGTTCCTGATGAAAACCGCCGAAACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCGTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATcgc                 >  1:107039/1‑239 (MQ=255)
                                                                                                                                                                                                                            cAATTACCGCAACCGAACCTCGGCGTGCGCACTAACGCCTTGATCGACACGCCGTTCCTGATGAAAACCGCCGAAACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCGTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATcgc                 >  2:707851/1‑239 (MQ=255)
                                                                                                                                                                                                                             aatcccccaacccGAACCTCGGCGTGCGCACTAACGCCTTGAGCGACACGCCGTTCCGGATGAAAACCGCCGAAACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCGTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATcgc                 <  2:1292573/227‑1 (MQ=255)
                                                                                                                                                                                                                             aaTTACCGCAACCGAACCTCGGCGTGCGCACTAACGCCTTGATCGACACGCCGTTCCTGATGAAAACCGCCGAAACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCGTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATcgc                 <  1:460580/238‑1 (MQ=255)
                                                                                                                                                                                                                             aaTTACCGCAACCGAACCTCGCCGTGCGCACTAACGCCTTGATCGACACGCCGTTCCTGATAAAAACCGCCGAAACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCGTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATcgcg                <  1:835623/239‑1 (MQ=255)
                                                                                                                                                                                                                                      acccGACCCGCGGCGTGCCCACAACCCCTTTAGTCGACACGCCGTTCCTGATGAAAACCGCCGAAACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCGTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAAt       <  2:465636/237‑1 (MQ=255)
                                                                                                                                                                                                                                      aaCCGACCCTCGGCGTGCCCACAAGCGCCTTGATCGACACGCCTTTCCTGATGAAAACCGCCGAAACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTTGCTGGAAGATGCGCGCGACTATTCCGTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAAt       <  2:543794/239‑1 (MQ=255)
                                                                                                                                                                                                                                      aaCCGACCCACGGCGTGCGCACTAACGCTTTGTTCGACACGCCGTTCCTGATGAAAACCGCCGAAACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCGTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAAt       <  1:487207/239‑1 (MQ=255)
                                                                                                                                                                                                                                      aaCCGAACCTCGGCGTGCGCACTAACGCCTTGATCGACACGCCGTTCCTGATGAAAACCGCCGAAACCATTCGCTTCGGTACCTGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCGTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTaa        >  1:926726/1‑238 (MQ=255)
                                                                                                                                                                                                                                       aCCGAACCTCGGCGTGCGCACTACCGCCTTGATCGACACGCCGTTCCTGATGAAAACCGCCGAAACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTAtt                                                                              <  1:1027795/167‑1 (MQ=255)
                                                                                                                                                                                                                                       aCCGAACCTCGGCGTGCGCACTAACGCCTTGATCGACACGCCGTTCCTGATGAAAACCGCCGAAACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTAtt                                                                              >  2:1027795/1‑167 (MQ=255)
                                                                                                                                                                                                                                       aCCGAACCTCGGCGTGCGCACTAACGCCTTGATCGACACGCCGTTCCTGATGAAAACCGCCGAAACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCGTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTaa        >  2:907208/1‑237 (MQ=255)
                                                                                                                                                                                                                                           aaCCTCGGCGTGCGCACTAACGCCTTGATCGACACGCCGTTCCTGATGAAAACCGCCGAAACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCGTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATct     >  1:711948/1‑236 (MQ=255)
                                                                                                                                                                                                                                           aaCCTCGGCGTGCGCACTAACGCCTTGATCGACACGCCGTTCCTGATGAAAACCGCCGAAACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCGTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGGTTAATct     >  1:704609/1‑236 (MQ=255)
                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                              
TCATTAACCCAGGGCGAAGATGCGGCGTTCCTGAAAGAACTGCTCGAATCTTTATGGGTGAAATGCAACGACATCGTGCTGTTGCGCTCCACCAGTAGCGCGCGTTATTTCGCCGGTTTCCCGACCGGCTATACCGCACTGCTCGGCGGGTTAACCGAGAACGGACGTAGCGCGGTGAACGTGCTTTCGTTCCTTTGCCTTGACGCCTATCAAAGCGTGCAATTACCGCAACCGAACCTCGGCGTGCGCACTAACGCCTTGATCGACACGCCGTTCCTGATGAAAACCGCCGAAACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCGTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCT  >  NC_000913/4144841‑4145313

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: