Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,465,778 T→C 10.2% I135V (ATC→GTC)  treB ← trehalose‑specific PTS enzyme: IIB and IIC component

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,465,7780TC10.2% 259.2 / 3.8 94I135V (ATC→GTC) treBtrehalose‑specific PTS enzyme: IIB and IIC component
Reads supporting (aligned to +/- strand):  ref base T (70/12);  new base C (2/8);  total (72/22)
Fisher's exact test for biased strand distribution p-value = 4.64e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

CCGGCAGCTGCTGCCCGAGCAGATAAGCGTTCATCAGCTGTGGAGAAACCAGTGTCACGCCAAGCACGATACCAAGGATCGGCGTGCCGCCCATTTTTTTCACCGCTGACCAGCAAATACCGACCGGCAGGTAGAAGAAGATCGCTTCACCGATCAACCACAGAAAATCGTAGATCGTTTGCAGGGAAGGGTACATTTGCGCCAGCGTCTGACCGTTGCTCATGGGCAAA‑TCGCCGATCACATTGCGAAAACCGAGG‑ATC‑AAACCGCCGCTAATCAACGCGGGCAGCAACGGGAAGAAGATCACCGCGAAATGAGAGATCAACTGCTCATGCCATTTCATATTATGCCGGGCGGCTTTTTTTACCTGCTCTTTATCAACCTGCGCCTGTCCGGTTGACGCAATCAGTGCTTGATAGTAATCACCCACGTTGGTGCCAATCACCACCTGAAATTGCCCGGCATTGGTGAAACAG  >  NC_000913/4465541‑4466013
                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                             
ccGGCAGCTGCTGCCCGAGCAGATAAGCGTTCATCAGCTGTGGAGAAACCAGTGTCACGCCAAGCACGATACCAAGGATCGGCGTGCCGCCCATTTTTTTCACCGCTGACCAGCAAATACCGACCGGCAGGTAGAAGAAGATCGCTTCACCGATCAACCACAGAAAATCGTAGATCGTTTGCAGGGAAGGGGACATTTGCGCCAGCGTCTGACCGTTGCTCATGGGCAAA‑TCGCCGATc                                                                                                                                                                                                                                              >  1:383985/1‑239 (MQ=255)
ccGGCAGCTGCTGCCCGAGCAGATAAGCGTTCATCAGCTGTGGAGAAACCAGTGTCACGCCAAGCACGATACCAAGGATCGGCGTGCCGCCCATTTTTTTCACCGCTGACCAGCAAATACCGACCGGCAGGTAGAAGAAGATCGCTTCACCCATCAACCACAGAAAATCGTAGATCGTTTGCATGGAAGGGTACATTTGCGCCAGCGTCTGACCGTTGCCCATGGGCAAA‑TAGCCGAt                                                                                                                                                                                                                                               >  2:595532/1‑238 (MQ=255)
      gctgctGCCCGAGCAGATAAGCGTTCATCAGCTGTGGAGAAACCAGTGTCACGCCAAGCACGATACCAAGGATCGGCGTGCCGCCCATTTTTTTCACCGCTGACCAGCAAATACCGACCGGCAGGTAGAAGAAGATCGCTTCACCGATCAACCACAGAAAATCGTAGATCGTTTGCAGGGAAGGGTACATTTGCGCCAGCGTCTGACCGTTGCTCATGGGCAAA‑TCGCCGATCACAtt                                                                                                                                                                                                                                         >  1:493444/1‑238 (MQ=255)
          ctgcCCGAGCAGATAAGCGTTCATCAGCTGTGGAGAAACCAGTGTCACGCCAAGCACGATACCAAGGATCGGCGTGCCGCCCATTTTTTTCACCGCTGACCAGCAAATACCGACCGGCAGGTAGAAGAAGATCGCTTCACCCATCCACCACAGAAAATCGTAGATCGTTTGCAGGGAAGGGTACATTTGCGCCAGCGTCTGACCGTTGCTCATGGGCAAA‑TCGCCGATCACATTGCGaa                                                                                                                                                                                                                                    >  2:571222/1‑239 (MQ=255)
            gcCCGAGCAGATAAGCGTTCATCAGCTGTGGAGAAACCAGTGTCACGCCAAGCACGATACCAAGGATCGGCGTGCCGCCCATTTTTTTCACCGCTGTCCAGCAAATACCGACCGGCAGGTAGAAGAAGATCGCTTCACCGATCAACCACAGAAAATCGTAGATCGTTTGCAGGGAAGGGTACATTTGCGCCAGCGTCTGACCGTTGCTCATGGGCAAA‑TCGCCGATCACATTGCGaaaa                                                                                                                                                                                                                                  <  1:761163/239‑1 (MQ=255)
              ccGAGCAGATAAGCGTTCATCAGCTGTTGAGAAACCAGTGCCACGCCAAGCACGATACCAAGGAGCGGCGTGCCGCCCATTTTTTTCACCGCTGACCAGCAAATACCGACCGGCAGGTAGAAGAAGATCGCTTCACCGATCAACCACAGAAAATCGTAGATCGTTTGCAGGGAAGGGTACATTTGCGCCAGCGTCTGACCGTTGCTCATGGGCAAA‑TCGCCGATCACATTGCGAAAAcc                                                                                                                                                                                                                                <  1:251478/239‑1 (MQ=255)
               cGAGCAGATAAGCGTTCATCAGCTGTGGAGAAACCAGTGTCACGCCAAGCACGATACCAAGGATCGGCGTGCCGCCCATTTTTTTCACCGCTGACCAGCAAATACCGACCGGCAGGTAGAAGAAGATCGCTTCACCGATCAACCACAGAAAATCGTAGATCGTTTGCAGGGAAGGGTACATTTGCGCCAGCGTCTGACCGTTGCTCATGGGCAAA‑TCGCCGATCACATTGCGAAAACCg                                                                                                                                                                                                                               <  1:104519/239‑1 (MQ=255)
               cGAGCAGATAAGCGTTCATCAGCTGTGGAGAAACCAGTGTCACGCCAAGCACGATACCAAGGATCGGCGTGCCGCCCATTTTTTTCACCGCTGACCAGCAAATACCGACCGGCAGGTAGAAGAAGATCGCTTCACCGATCAACCACAGAAAATCGTAGATCGTTTGCAGGGAAGGGTACATTTGCGCCAGCGTCTGACCGTTGCTCATGGGCAAA‑TCGCCGATCACATTGCGAAAACCg                                                                                                                                                                                                                               <  2:437125/239‑1 (MQ=255)
                  gCAGATAAGCGTTCATCAGCTGTGGAGAAACCAGTGTCACGCCAAGCACGATACCAAGGATCGGCGTGCCGCCCATTTTTTTCACCGCTGACCAGCAAATACCGACCGGCCGGGAGAAGAAGATCGCTTCACCGATCAACCACAGAAAATCGTAGAACGTTTGCAGGGAAGGGCACATTTGCGCCAGCGTCTGACCCTTGCTCATGGGCAAA‑TCGCCGATCACATTGCGAAAACCGAgg                                                                                                                                                                                                                            >  1:579644/1‑239 (MQ=255)
                                atcaGCTGTGGAGAAACCAGTGTCACGCCAAGCACGATACCAAGGATCGGCGTGCCGCCCATTTTTTTCACCGCTGACCAGCAAATACCGACCGGCAGGTAGAAGAAGATCGCTTCACCGATCAACCACAGAAAATCGTAGATCGTTTTCAGGGAAGGGAACATTTGCGCCAGCGTCTGACCGTTGCTCATGGGCAAA‑TCGCCGATCACATTGCGAAACCCGAGG‑ATC‑AAACCGCCGCt                                                                                                                                                                                                            >  1:201026/1‑239 (MQ=255)
                                atcaGCTGTGGAGAAACCAGTGTCACGCCAAGCACGATACCAAGGATCGGCGTGCCGCCCATTTTTTTCACCGCTGACCAGCAAATACCGACCGGCAGGTAGAAGAAGATCGCTTCACCGATCAACCACAGAAAATCGTAGATCGTTTGCAGGGAAGGGTACATTTGCGCCCGCGTCTGACCGTTGCTCATGGGCAAA‑TCGCCGATAACATTACGAAAACCGAGG‑ATC‑AAACCGCCGCt                                                                                                                                                                                                            >  1:444632/1‑239 (MQ=255)
                                atcaGCTGTGGAGAAACCAGTGTCACGCCAAGCACGATACCAAGGATCGGCGTGCCGCCCATTTTTTTCACCGCTGACCAGCAAATACCGACCGGCAGGTAGAAGAAGATCGCTTCACCGATCAACCACAGAAAATCGTAGATCGTTTGCAGGGAAGGGTACATTTGCGCCAGCGTCTGACCGTTGCTCATGGGCAAA‑TCGCCGATCACATTGCGAAAACCGAGG‑ATC‑AAACCGCCGCt                                                                                                                                                                                                            >  2:183771/1‑239 (MQ=255)
                                atcaGCTGTGGAGAAACCAGTGTCACGCCAAGCACGATACCAAGGATCGGCGTGCCGCCCATTTTTTTCACCGCTGACCAGCAAATACCGACCGGCAGGGAGAAGAAGATCGCTTCACCGATCAACCACAGAAAATCGTAGATCGTTTGCAGGGAAGGGGAAATTTGCGCCAGCGTCTGACCGTTGCTCATGGGCAAA‑TCGCCGATCACATTGCGAAAACCGAGG‑GTC‑AAACCGCCCCt                                                                                                                                                                                                            >  2:84964/1‑239 (MQ=255)
                                        tgGAGAAACCAGTGTCACGCCAAGCACGATACCAAGGATCGGCGTGCCGCCCATTTTTTTCACCGCTGACCAGCAAATACCGACCGGCAGGTAGAAGAAGATCGCTTCACCGATCAACCACAGAAAATCGTAGATCGTTTGCAGGGAAGGGTACATTTGCGCCAGCGTCTGACCGTTGCTCATGGGCAAA‑TCGCCGATCACATTGCGAAAACCGAGG‑ATC‑AAACCGCCGCTAATCAAc                                                                                                                                                                                                     >  2:1350907/1‑238 (MQ=255)
                                        tgGAGAAACCAGTGTCACGCCAAGCACGATACCAAGGATCGGCGTGCCGCCCATTTTTTTCACCGCTGACCAGCAAATACCGACCGGCAGGTAGAAGAAGATCGCTTCACCGATCAACCACAGAAAATCGTAGATCGTTTGCAGGGAAGGGTACATTTGCGCCAGCGTCTGACCGTTGCTCATGGGCAAA‑TCGCCGATCACATTGCGAAAACCGAGG‑ATC‑AAACCGCCGCTAATCAAc                                                                                                                                                                                                     >  1:828971/1‑238 (MQ=255)
                                        tgGAGAAACCAGTGTCACGCCAAGCACGATACCAAGGATCGGCGTGCCGCCCATTTTTTTCACCGCTGACCAGCAAATACCGACCGGCAGGTAGAAGAAGATCGCTTCACCGATCAACCACAGAAAATCGTAGATCGTTTGCAGGGAAGGGGACATTTGCGCCAGCGTCTGACCGTTGCTCATGGGCAAA‑TCGCCGATCACATTGCGAAAACCGAGG‑ATC‑AAACCGCCGCTAATCAAc                                                                                                                                                                                                     >  1:41061/1‑238 (MQ=255)
                                          gagaAACCAGTGTCACGCCAAGCACGATACCAAGGATCGGCGTGCCGCCCATTTTTTTCACCGCTGACCAGCAAATACCGACCGGCAGGTAGAAGAAGATCGCTTCACCGATCAACCACAGAAAATCGTAGATCGTTTGCAGGGAAGGGTACATTTGCGCCAGCGTCTGACCGTTGCTCATGGGCAAA‑TCGCCGATCACATTGCGAAAACCGAGG‑ATC‑AAACCGCCGCTAATCAAcgcg                                                                                                                                                                                                  >  1:71103/1‑239 (MQ=255)
                                               aCCAGTGTCACGCCAAGCACGATACCAAGGATCGGCGTGCCGCCCATTTTTTTCACCGCTGACCAGCAAATACCGACCGGCAGGTAGAAGAAGATCGCTTCACCGATCAACCACAGAAAATCGTAGATCGTTTGCAGGGAAGGGTACATTTGCGCCAGCGTCTGACCGTTGCTCATGGGCAAA‑TCGCCGATCACATTGCGAAAACCGAGG‑ATC‑AAACCGCCGCTAATCAACGCGGgca                                                                                                                                                                                              >  1:732966/1‑238 (MQ=255)
                                                  aGTGTCACGCCAAGCACGATACCAAGGATCGGCGTGCCGCCCATTTTTTTCACCGCTGACCAGCAAATACCGACCGGCAGGTAGAAGAAGATCGCTTCACCGATCAACCACAGAAAATCGTAGATCGTTTGCAGGGAAGGGTACATTTGCGCCAGCGTCTGACCGTTGCTCATGGGCAAA‑TCGCCGATCACATTGCGAAAACCGAGG‑ATC‑AAACCGCCGCTAATCAACCCGGgcagca                                                                                                                                                                                           >  1:420171/1‑238 (MQ=255)
                                                    tgtCACGCCAAGCACGATACCAAGGATCGGCGTGCCGCCCATTTTTTTCACCGCTGACCAGCAAATACCGACCGGCAGGTAGAAGAAGATCGCTTCACCGATCAACCACAGAAAATCGTAGATCGTTTGCAGGGAAGGGTACATTTGCCCCAGCGTCTGACCGTTGCTCATGGGCAAA‑TCGCCGATCACATTGCGAAAACCGAGG‑ATC‑AAACCGCCGCTAATCAACGCGGGCAGCAACg                                                                                                                                                                                        >  2:1096134/1‑239 (MQ=255)
                                                         cGCCAAGCACGATACCAAGGATCGGCGTGCCGCCCATTTTTTTCACCGCTGACCAGCAAATACCGACCGGCAGGTAGAAGAAGATCGCTTCACCGATCAACCACAGAAAATCGTAGATCGTTTGCAGGGAAGGGTACATTTGCGCCAGCGTCTGACCGTTGCTCATGGGCAAA‑TCGCCGATCACATTGCGAAAACCGAGG‑ATC‑AAACCGCCGCTAATCAACGCGGGCCGCAACGGgaag                                                                                                                                                                                   >  1:90643/1‑239 (MQ=255)
                                                         cGCCAAGCACGATACCAAGGATCGGCGTGCCGCCCATTTTTTTCACCGCTGACCAGCAAATACCGACCGGCAGGTAGAAGAAGATCGCTTCACCGATCAACCACAGAAAATCGTAGATCGTTTGCAGGGAAGGGGACATTTGCGCCAGCGTCTGACCGTTGCTCATGGGCAAA‑TCGCCGATCACATTGCGAAAACCGAGG‑ATC‑AAACCGCCGCTAATCAACGCGGGCAGCAACGGgaag                                                                                                                                                                                   >  2:243448/1‑239 (MQ=255)
                                                         cGCCAAGCACGATACCAAGGATCGGCGTGCCGCCCATTTTTTTCACCGCTGACCAGCAAATACCGACCGGCAGGGAGAAGAAGATCGCTTCACCGATCAACCACAGAAAATCGTAGATCGTTTGCAGGGAAGGGTACATTTGCGCCAGCGTCTGACCCGTGATCATGGGCAAA‑TCGCCGATCACATTGCGAAAACCGAGG‑ATC‑AAACAGCCGCTCAACAACTCGGGCAGCAACGGgaag                                                                                                                                                                                   >  2:1009193/1‑239 (MQ=255)
                                                              aGCACGATACCAAGGATCGGCGTGCCGCCCATTTTTTTCACCGCTGACCAGCAAATACCGACCGGCAGGTAGAAGAAGATCTCTTCACCGATCAACCACAGAAAATCGTAGATCGTTTGCAGGGAAGGGCACATTTGCGCCAGCGTCTGACCGTTGCTCATGGGCAAA‑TCGCCGATCACATTGCGAAAACCGAGG‑ATC‑AAACCGCCGCCAATCAAAGCGGGCAGCAACGGGAagaaga                                                                                                                                                                               >  2:1268313/1‑238 (MQ=255)
                                                                cACGATACCAAGGATCGGCGTGCCGCCCATTTTTTTCACCGCTGACCAGCAAATACCGACCGGCAGGTAGAAGAAGATCGCTTCACCGATCAACCACAGAAAATCGTAGATCGTTTGCAGGGAAGGGGACATTTGCGCCAGCGTCTGACCGTTGCTCATGGGCAAA‑TCGCCGATCACATTGCGAAAACCGAGG‑ATC‑AAACCGCCGCTAATCAACGCGGGCAGCAACGGGAAGAAGATc                                                                                                                                                                             >  1:321432/1‑238 (MQ=255)
                                                                    aTACCAAGGATCGGCGTGCCGCCCATTTTTTTCACCGCTGACCAGCAAATACCGACCGGCAGGTAGAAGAAGATCGCTTCACCGATCAACCACAGAAAATCGTAGATCGTTTGCAGGGAAGGGTACATTTGCGCCAGCGTCTGACCGTTGCTCATGGGCAAA‑TCGCCGATCACATTGCGAAAACCGAGG‑ATC‑AAACCGCCGCTAATCAACGCGGGCAGCAACGGGAAGAAGATCACcgc                                                                                                                                                                        >  1:1362381/1‑239 (MQ=255)
                                                                         agggAGCGGGGGGCCGCCTATTTTTTCCACGGCTAACAAAAAAAAACCGGCCGGAAGGTAGAAGAAGATCGCTTCACCGATAAACCACAAAAAATCGAAGTTGGTTGGCAGGGAAGGGTACATTTGGCCAAGGGCCGGACCGTTGCTAATGGGAAAA‑CCGCCGATCAAATTGAGAAAACAGAGG‑ATA‑AACCCGCCGCTAATCAACGCGGGCAGCAACGGGAAGAAGATCACCGCGAAAt                                                                                                                                                                   <  2:732966/237‑1 (MQ=255)
                                                                          aGGATCGGCGTGCCGCCCATTTTTTTCACCGCTGACCAGCAAATACCGACCGGCAGGGAGAAGAAGATCGCTTCACCGATCAACCACAGAAAATCGTAGATCGTTTGCAGGGAAGGGTACATTTGCGCCAGCGTCTGACCGCTGCTCATGGGCAAA‑TCGCCGATCACATTGCGAAAACCGAGG‑ATC‑AAACCGCCGCTAATCAACGCGGGCAGCAACGGGAAGAAGATCACCGCGAAATg                                                                                                                                                                  >  1:648986/1‑239 (MQ=255)
                                                                               cGGCGTGCCGCCCATTTTTTTCACCGCTGACCAGCAAATACCGACCGGCAGGTAGAAGAAGATCGCTTCACCGATCAACCACAGAAAATCGTAGATCGTTTGCAGGGAAGGGTACATTTGCGCCAGCGTCTGACCGTTGCTCATGGGCAAA‑TCGCCGATCACATTGCGAAAACCGAGG‑ATC‑AAACCGCCGCTAATCAACGCGGGCAGCAACGGGAAGAAGATCACCGCGAAATgagaga                                                                                                                                                             >  1:828327/1‑239 (MQ=255)
                                                                               cGGCGTGCCGCCCATTTTTTTCACCGCTGACCAGCAAATACCGACCGGCAGGTAGAAGAAGATCGCTTCACCGATCAACCACAGAAAATCGTAGATCGTTTGCAGGGAAGGGTACATTTGCGCCAGCGTCTGACCGTTGCTCATGGGCAAA‑TCGCCGATCACATTGCGAAAACCGAGG‑ATC‑AAACCGCCGCTAATCAAAGCGGGCAGCAACGGGAAGAAGATCACCGCGAAATgagcaa                                                                                                                                                             >  2:1041853/1‑236 (MQ=255)
                                                                               cGGCGTGCCGCCCATTTTTTTCACCGCTGACCAGCAAATACCGACCGGCAGGTAGAAGAAGATCGCTTCACCGATCAACCACAGAAAATCGTAGATCGTTTGCAGGGAAGGGTACATTTGCGCCAGCGTCTGACCGTTGCTCATGGGCAAA‑TCGCCGACCACATTGCGAAAACCGAGG‑ATC‑AAACCGCCGCTAATCAACGCGGGCAGCAACGGGAAGAAGATCACCGCGAAATgagaga                                                                                                                                                             >  1:993610/1‑239 (MQ=255)
                                                                               cGGCGTGCCGCCCATTTTTTTCACCGCTGACCAGCAAATACCGACCGGCAGGGAGAAGAAGATCGCTTCACCGATCAACCCCAGAAAATCGTAGATCGTTTGCAGGGAAGGGTACATTTTCGCCAGCGTCTGACCGTTGCTCATGGGCAAA‑TCGCCGCTCACATTTCGAAAACCGAGG‑ATC‑AAACCGCCGCCACTCAACGCGGGCAGCAAAGGGAAGAAGATAACAGCGAAATgagagc                                                                                                                                                             >  2:1743/1‑238 (MQ=255)
                                                                                ggCGTGCCGCCCATTTTTTTCACCGGTGACCAGCAAAGACCGACCGGCAGGAAGAAGAAGATCGCTTCACCGATCAACCACAGAAAATCGGAGATCGTTTGCAGGGAAGGGTACATTTACCCCAGCGTCTGACCGTTGCTCATGGGCTAA‑TCGCATATCACATTGCGAAAACCGCGG‑ATC‑AAACCGCCGCTAATCAACGCGGGCAGAAACGGGAAGAAAAACACCGCGaacagatagc                                                                                                                                                             >  2:337447/1‑230 (MQ=255)
                                                                                ggCGTGCCGCCCATTTTTTTCACCGCTGACCAGCAAATACCGACCGGCAGGAAGAAGAAGATTGCTTCAACGATCAACCACAGAAAAACGGAGATAGGTTGCAGGGGAGGCTACCTTTGCGCCAGCACCTGACCGATGCTCATGGGTCAA‑TAGCCGATAACCATGCGAAAACCGAAG‑CGC‑AAATCGCCGCCAATCCACACGGGCGTCACCGTGAACAAACTCACCGCGAAATGAGAGAt                                                                                                                                                            >  2:988026/1‑239 (MQ=255)
                                                                                    tGCCGCCCATTTTTTTCACCGCTGACCAGCAAATACCGACCGGCAGGTAGAAGAAGATCGCTTCACCGATCAACCACAGAAAATCGTAGATCGTTTGCAGGGAAGGGTACATTTGCGCCAGCGTCTGACCGTTGCTCATGGGCAAA‑TCGCCGATTACATTGCGAAAACCGAGG‑ATC‑AAACCGCCGCTAATCAACGCGGGCAGCAACGGGAAGAAGATCACCGCGAAATGAGGGATCa                                                                                                                                                          >  1:663978/1‑237 (MQ=255)
                                                                                       cgccCATTTTTTTCACCGCTGACCAGCAAATACCGACCGGCAGGTAGAAGAAGATCGCTTCACCGATTAACCACAGAAAATCGTAGATCGTTTGCAGGGAAGGGTACATTTGCGCCAGCGTCTGACCGTTGCTCATGGGCAAA‑TCCCCGATCACATTGCGAAAACCGAGG‑ATC‑AAACCGCCGCTAATCAACGCGGGCAGCAACGGGAAGAAGATCACCGCGAAATGAGAGATCAACt                                                                                                                                                       >  1:949345/1‑237 (MQ=255)
                                                                                        ccccATTTTTTCCCCCGCAGACAAGAAAACACCGACCGGCAGGAAGAAGAAGATCGCTTCGCCGATCACCCACAGAAAATGGTAGATCTTTTGAGGGGAGGGGTACATTTGCGCAAGCTCCGGACCTTTACCCAGGGGAAAA‑TCGCCGATCACATTGCAAAAACCGAGG‑ACC‑AAACCGCCGCTAATCAACGCGGGCAGCAACGGGAAGAAGATCACCGCGAAATGAGAGATCAACTGCt                                                                                                                                                    <  2:90643/238‑1 (MQ=255)
                                                                                        acctaTTTTTTCCCCCCCTCACAAGCAAACACCGCCGGGCAGGTAAAAGAAGATCGCTTCCCCGACCAACCACAGAAAAGCGTAGATCGTTTGCGGGGAAGGGTACATTGGCGCCAGCGTCTGCCGGTTGCTGAGGGGAAAA‑CCGCCGACCACATTGAAAAAACCGAGG‑AAC‑AAACCGCCGCTAATCAACGCGGGCAGCAACGGGAAGAAGATCACCGCGAAATGAGAGATCAACTGCt                                                                                                                                                    <  2:1422494/235‑1 (MQ=255)
                                                                                         cccATTTTTTTCACCGCTGACCAGCAAATACCGACCGGCAGGGAGAAGAAGATCGCTTCACCGCTCAACCACAGAAAATCGGAGATCGTTTGTAGGGACGGGTACATTTGGGCCAGCGCATGGCCGTTGCTCATAAGCAAA‑TCGCCGATAACATTGCGCAAACCGAGG‑ATC‑AAACAGCCGCTAATCAACTCAGGCAGCCACGTGAAGAAGCTCTCCGCGAAATGAGAGATCAAcgtct                                                                                                                                                    >  2:1441027/1‑234 (MQ=255)
                                                                                           cATTTTTTTCACCGCTGACCAGCAAATACCGACCGGCAGGTAGAAGAAGATCGCTTCACCGATCAACCACAGAAAATCGTAGATCGTTTGCAGGGAAGGGTACATTTGCGCCAGCGTCTGACCGTTGCTCATGGGCAAA‑TCGCCGATCACATTGCGAAAACCGAGG‑ATC‑AAACCGCCGCTAATCAACGCGGGCAGCAACGGGAAGAAGATCACCGCGAAATGAGAGATCAACTGCTCAt                                                                                                                                                 >  1:1174820/1‑239 (MQ=255)
                                                                                            tttttttcCCCCGCGCACCACAAAATACCGCCCGGCAGGAAAAAGAAGATCGCTCCACCGACCAACCAAAGAAAATCGTAGATCTTTGGCGGGGAGGGGTACATTTGCCCCAGCGTCTGCCGGTTGCTCATGGGCAAA‑CCGCCGACCACATGGCAAAACCCAAGG‑ATA‑AAACCGCCGCTAATCAACGCGGGCAGCAACGGGAAGAAGATCACCGCGAAATGAGAGATCAACTGCTCATg                                                                                                                                                <  1:1386272/238‑1 (MQ=255)
                                                                                                 gttCCCCGCTCACCAGAAAACACCGACCGGGTGGGAAAAGAAGGTCGATCCCCCGACCCCCAAAAGAAAATGGTAGTTTGTTGGAGGGGAATGCTACATTTCGTCCAGCGTCTGACGGTTCTTCAGGGGCAAA‑GCGCAGACCACATTG‑GAAAACCGAAG‑AACAAAACCGCCGCTAATCAACGCGGGCAGCAACGGGAAGAAGATCACCGCGAAATGAGAGATCAACTGCTCATGCCAtt                                                                                                                                           <  2:1174820/238‑1 (MQ=255)
                                                                                                           gACCAGCAAATACCGACCGGCAGGTAGAAGAAGATCGCTTCACCGATCAACCACAGAAAATCGTAGATCGTTTGCAGGGAAGGGTACATTTGCGCCAGCGTCTGACCGTTGCTCATGGGCAAA‑TCGCCGATCACATTGCGAAAACCGAGG‑ATC‑AAACCGCCGCTAATCAACGCGGGCAGCAACGGGAAGAAGATCACCGCGAAATGAGAGATCAACTGCTCATGCCATTTCAtattat                                                                                                                                  >  1:1205456/1‑238 (MQ=255)
                                                                                                                 cAAATACCGACCGGCAGGTAGAAGAAGATCGCTTCACCGATCAACCACAGAAAATCGTAGATCGTTTGCAGGGAAGGGTACATTTGCGCCAGCGTCTGACCGTTGCTCATGGGCAAA‑TCGCCGATCACATTGCGAAAACCGAGG‑ATC‑AAACCGCCGCTAATCAACGCGGGCAGCAACGGGAAGAAGATCACCGCGAAATGAGAGATCAACTGCTCATGCCATTTCATATTATGCCGggc                                                                                                                           >  1:1002737/1‑239 (MQ=255)
                                                                                                                      accgaccgGCAGGTAGAAGAAGATCGCTTCACCGATCAACCACAGAAAATCGTAGATCGTTTGCAGGGAAGGGTACATTTGCGCCAGCGTCTGACCGTTGCTCATGGGCAAA‑TCGCCGATCACATTGCGAAAACCGAGG‑ATC‑AAACCGCCGCTAATCAACGCGGGCAGCAACGGGAAGAAGATCACCGCGAAATGAGAGATCAACTGCTCATGCCATTTCATATTATGCCGGGCGGCtt                                                                                                                      >  2:703736/1‑239 (MQ=255)
                                                                                                                      accgaccgGCAGGTAGAAGAAGATCGCTTCACCGATCAACCACAGAAAATCGTAGATCGTTTGCAGGGAAGGGTACATTTGCGCCAGCGTCTGACCGTTGCTCATGGGCAAA‑TCGCCGATCACATTGCGAAAACCGAGG‑ATC‑AAACCGCCGCTAATCAACGCGGGCAGCAACGGGAAGAAGATCACCGCGAAATGAGAGATCAACTGCTCATGCCATTTCATATTATGCCGGGCGGCtt                                                                                                                      >  1:291159/1‑239 (MQ=255)
                                                                                                                          accgGCAGGTAGAAGAAGATCGCTTCACCGATCAACCACAGAAAATCGTAGATCGTTTGCAGGGAAGGGTACATTTGCGCCAGCGTCTGACCGTTGCTCATGGGCAAA‑TCGCCGATCACATTGCGAAAACCGAGG‑ATC‑AAACCGCCGCTAATCAACGCGGGCAGCAACGGGAAGAAGATCACCGCGAAATGAGAGATCAACTGCTCATGCCATTTCATATTATGCCGGGCGGCcttttt                                                                                                                  >  1:1321087/1‑239 (MQ=255)
                                                                                                                              gagggaagaagaggaTCGCTTACCCGAAAAACAAAAAAAAGTCGTAGATGGTTGGAAGCGAAGGGTACA‑TTGGGCCACGGGCGTGTCGTTGGCTTTGGGAAAACTCGGCGACCAGTTTGAAAACACCGAGG‑AAA‑AAACCGCCGCTAATCAACGCGGGCAGCAACGGGAAGAAGATCACCGCGAAATGAGAGATCAACTGCTCATGCCATTTCATATTATGCCGGGCGGCTTTTTTTAc                                                                                                               <  2:118831/232‑1 (MQ=255)
                                                                                                                                   tAGAAGAAAACCGCTACACCAATCAACCAAAGAAAGTCTGAGATCTTTTGAGGGGAGGGGTACTTTGGCGCCAGCGTCCGACGGTTCGTCAGGAAAAAA‑GCCACGAACACATGGAAAAAACAGAGA‑AAC‑AAACCGCCGCTAATCAACGCGGGCAGCAACGGGAAGAAGATCACCGCGAAATGAGAGATCAACTGCTCATGCCATTTCATATTATGCCGGGCGGCTTTTTTTACCTGct                                                                                                          <  2:321432/238‑1 (MQ=255)
                                                                                                                                        gaagaTCGCTTCACCGATCAACCACAGAAAATCGTAGATCGTTTGCAGGGAAGGGTACATTTGCGCCAGCGTCTGACCGTTGCTCATGGGCAAA‑TCGCCGATCACATTGCGAAAACCGAGG‑ATC‑AAACCGCCGCTAATCAACGCGGGCAGCAACGGGAAGAAGATCACCGCGAAATGAGAGATCAACTGCTCATGCCATTTCATATTATGCCGGGCGGCTTTTTTTACCTGCTCTTTa                                                                                                     >  1:406975/1‑238 (MQ=255)
                                                                                                                                            aTCGCTTCACCGATCAACCACAGAAAATCGTAGATCGTTTGCAGGGAAGGGTACATTTGCGCCAGCGTCTGACCGTTGCTCATGGGCAAA‑TCGCCGATCACATTGCGAAAACCGAGG‑ATC‑AAACCGCCGCTAATCAACGCGGgcagca                                                                                                                                                                                           >  1:1243116/1‑148 (MQ=255)
                                                                                                                                            aTCGCTTCACCGATCAACCACAGAAAATCGTAGATCGTTTGCAGGGAAGGGTACATTTGCGCCAGCGTCTGACCGTTGCTCATGGGCAAA‑TCGCCGATCACATTGCGAAAACCGAGG‑ATC‑AAACCGCCGCTAATCAACGCGGGCAGCAACGGGAAGAAGATCACCGCGAAATGAGAGATCAACTGCTCATGCCATTTCATATTATGCCGGGCGGCTTTTTTTACCTGCTCTTTATCaa                                                                                                 >  1:822142/1‑238 (MQ=255)
                                                                                                                                                cTTCACCGATCAACCACAGAAAATCGTAGATCGTTTGCAGGGAAGGGTACATTTGCGCCAGCGTCTGACCGTTGCTCATGGGCAAA‑TCGCCGATcac                                                                                                                                                                                                                                            <  2:743284/97‑1 (MQ=255)
                                                                                                                                                cTTCACCGATCAACCACAGAAAATCGTAGATCGTTTGCAGGGAAGGGTACATTTGCGCCAGCGTCTGACCGTTGCTCATGGGCAAA‑TCGCCGATcac                                                                                                                                                                                                                                            >  1:743284/1‑97 (MQ=255)
                                                                                                                                                cTTCACCGATCAACCACAGAAAATCGTAGATCGTTTGCAGGGAAGGGTACATTTGCGCCAGCGTCTGACCGTTGCTCATGGGCAAA‑TCGCCGATCACATTGCGAAAACCGAGG‑ATC‑AAACCGCCGCTAATCAACGCGGGCAGCAACGGGAAGAAGATCACCGCGAAATGAGAGATCAACTGCTCATGCCATTTCATATTATGCCGGGCGGCTTTTTTTACCTGCTCTTTATCAACCTgc                                                                                            >  1:344366/1‑239 (MQ=255)
                                                                                                                                                cTTCACCGATCAACCACAGAAAATCGTAGATCGTTTGCAGGGAAGGGTACATTTGCGCCAGCGTCTGACCGTTGCTCATGGGCAAA‑TCGCCGATCACATTGCGAAAACCGAGG‑ATC‑AAACCGCCGCTAATCAACGCGGGCAGCAACGGGAAGAAGATCACCGCGAAATGAGAGATCAACTGCTCATGCCATTTCATATTATGCCGGGCGGCTTTTTTTACCTGCTCTTTATCAACCTgc                                                                                            >  1:1029606/1‑239 (MQ=255)
                                                                                                                                                cTTCACCGATCAACCACAGAAAATCGTAGATCGTTTGCAGGGAAGGGTACATTTGCGCCAGCGTCTGACCGTTGCTCATGGGCAAA‑TCGCCGATCACATTGCGAAAACCGAGG‑ATC‑AAACCGCCGCTAATCAACGCGGGCAGCAACGGGAAGAAGATCACCGCGAAATGAGAGATCAACTGCTCATGCCATTTCATATTATGCCGGGCGGCTTTTTTTACCTGCTCTTTATCAACCTgc                                                                                            >  2:649859/1‑239 (MQ=255)
                                                                                                                                                cTTCACCGATCAACCACAGAAAATCGTAGATCGTTTGCAGGGAAGGGTACATTTGCGCCAGCGTCTGACCGTTGCTCATGGGCAAA‑TCGCCGATCACATTGCGAAAACCGAGG‑ATC‑AAACCGCCGCTAATCAACGCGGGCAGCAACGGGAAGAAGATCACCGCGAAATGAGAGATCAACTGCTCATGCCATTTCATATTATGCCGGGCGGCTTTTTTTACCTGCTCTTTATCAACCTgc                                                                                            >  1:572626/1‑239 (MQ=255)
                                                                                                                                                        aTCAACCACAGAAAATCGTAGATCGTTTGCAGGGAAGGGTACATTTGCGCCAGCGTCTGACCGTTGCTCATGGGCAAA‑TCGCCGATCACATTGCGAAAACCGAGG‑ATC‑AAACCGCCGCTAATCAACGCGGGCAGCAACGGGAAGAAGATCACCGCGAAATGAGAGATCAACTGCTCATGCCATTTCATATTATGCCGGGCGGCTTTTTTTACCTGCTCTTTATCAACCTGCGCCTGTcc                                                                                    >  1:759543/1‑239 (MQ=255)
                                                                                                                                                           aaCCACAGAAAATCGTAGATCGTTTGCAGGGAAGGGTACATTTGCGCCAGCGTCTGACCGTTGCTCATGGGCAAA‑TCGCCGATCACATTGCGAAAACCGAGG‑ATC‑AAACCGCCGCTAATCAACGCGGGCAGCAACGGGAAGAAGATCACCGCGAAATGAGAGATCAACTGCTCATGCCATTTCATATTATGCCGGGCGGCTTTTTTTACCTGCTCTTTATCAACCTGCGCCTGTCCGGt                                                                                 >  2:1096679/1‑239 (MQ=255)
                                                                                                                                                               aaaaaaatgcGTAGATGTTTTGGGGGAAAGGTTACATTGGGCCCGGCGTCTGCCGGTGGCTAAGGGGAAAG‑CCGCCGATCAAATTGCAAAACCCGAGA‑ACC‑AAACCGCCGCTAATCAACGCGGGCAGCAACGGGAAGAAGATCACCGCGAAATGAGAGATCAACTGCTCATGCCATTTCATATTATGCCGGGCGGCTTTTTTTACCTGCTCTTTATCAACCTGCGCCTGTCCGGTTGAc                                                                             <  1:243448/230‑1 (MQ=255)
                                                                                                                                                               aaaGAAAAGCGTAGATCGTTGGCGGGGAAGGGTACATTGGCGCCAGCTGCGGACCGTTGCTCAGGGGAAAA‑CCGCGGACCACATTGC‑AAAACCGGGGAATA‑AACCCGCCGCTAATCAACGCGGGCAGCAACGGGAAGAAGATCACCGCGAAATGAGAGATCAACTGCTCATGCCATTTCATATTATGCCGGGCGGCTTTTTTTACCTGCTCTTTATCAACCTGCGCCTGTCCGGTTGAc                                                                             <  1:571222/237‑1 (MQ=255)
                                                                                                                                                                caGAAAATCGTAGATCGTTTGCAGGGAAGGGTACATTTGCGCCAGCGTCTGACCGTTGCTCATGGGCAAA‑TCGCCGATCACATTGCGAAAACCGAGG‑ATC‑AAACCGCCGCTAATCAACGCGGGCAGCAACGGGAAGAAGATCACCGCGAAATGAGAGATCAACTGCTCATGCCATTTCATATTATGCCGGGCGGCTTTTTTTACCTGCTCTTTATCAACCTGCGCCTGTCCGGTTGAc                                                                             >  2:1204928/1‑238 (MQ=255)
                                                                                                                                                                caGAAAATCGTAGATCGTTTGCAGGGAAGGGTACATTTGCGCCAGCGTCTGACCGTTGCTCATGGGCAAA‑TCGCCGATCACATTGCGAAAACCGAGG‑ATC‑AAACCGCCGCTAATCAACGCGGGCAGCAACGGGAAGAAGATCACCGCGAAATGAGAGATCAACTGCTCATGCCATTTCATATTATGCCGGGCGGCTTTTTTTACATCCTCTTTATCAACCTGCGCCTTTTCGGTTGAc                                                                             >  2:1416298/1‑238 (MQ=255)
                                                                                                                                                                    aaaTCGTAGATGGTGTGCAGGGAAGGTTACATTGGCCCCAGCTCCCGCCCGTTGCCAATGGACAAA‑CCGCCGACCAAATTGCAAAAACCGAGA‑ATC‑AAACCGCCGCTAATCAACGCGGGCAGCAACGGGAAGAAGATCACCGCGAAATGAGAGATCAACTGCTCATGCCATTTCATATTATGCCGGGCGGCTTTTTTTACCTGCTCTTTATCAACCTGCGCCTGTCCGGTTGACGCAAt                                                                        <  1:1061426/239‑1 (MQ=255)
                                                                                                                                                                      attgTAGATCGTTTGGAGGGAGGGTTACATTTGCCCCAGCGTCTGACCTTTGCTCATGGGCAAA‑CGCCCGATCAAATTGCAAAACCCGAGA‑AAA‑AAACCGCCGCTAATCAACGCGGGCAGCAACGGGAAGAAGATCACCGCGAAATGAGAGATCAACTGCTCATGCCATTTCATATTATGCCGGGCGGCTTTTTTTACCTGCTCTTTATCAACCTGCGCCTGTCCGGTTGACGCAATCa                                                                      <  1:904355/236‑1 (MQ=255)
                                                                                                                                                                      aTCGTGTATCGTTTGCAGGGAAGGTTACATTTGCCCCAGCGTCTGACCGTTGCTAATGGACAAA‑TCGCCGATCACATGGCAAAAACCGAGA‑AAC‑AAACCGCCGCTAATCAACGCGGGCAGCAACGGGAAGAAGATCACCGCGAAATGAGAGATCAACTGCTCATGCCATTTCATATTATGCCGGGCGGCTTTTTTTACCTGCTCTTTATCAACCTGCGCCTGTCCGGTTGACGCAATCa                                                                      <  1:121761/239‑1 (MQ=255)
                                                                                                                                                                       tCGTAGATCGTTTGCAGGGAAGGGTACATTTGCGCCAGCGTCTGACCGTTGCTCATGGGCAAA‑TCGCCGATCACATTGCGAAAACCGAGG‑ATC‑AAACCGCCGCTAATCAACGCGGGCAGCAACGGGAAGAAGATCACCGCGAAATGAGAGATCAACTGCTCATGCCATTTCATATTATGCCGGGCGGCTTTTTTTACCTGCTCTTTATCAACCTGCGCCTGTCCGGTTGACGCAATCAg                                                                     >  2:987283/1‑239 (MQ=255)
                                                                                                                                                                          tAGATCGTTTGCAGGGAAGGGTACATTTGCGCCAGCGTCTGACCGTTGCTCATGGGCAAA‑TCGCCGATCACATTGCGAAAACCGAGG‑ATC‑AAACCGCCGCTAATCAACGCGGGCAGCAACGGGAAGAAGATCACCGCGAAATGAGAGATCAACTGCTCATGCCATTTCATATTATGCCGGGCGGCTTTTTTTACCTGCTCTTTATCAACCTGCGCCTGTCCGGTTGACGCAATCAgc                                                                    >  1:1203153/1‑236 (MQ=255)
                                                                                                                                                                             aTCGTTTGCAGGGAAGGGTACATTTGCGCCAGCGTCTGACCGTTGCTCATGGGCAAA‑TCGCCGATCACATTGCGAAAACCGAGG‑ATC‑AAACCGCCGCTAATCAACGCGGGCAGCAACGGGAAGAAGATCACCGCGAAATGAGAGATCAACTGCTCATGCCATTTCATATTATGCCGGGCGGCTTTTTTTACCTGCTCTTTATCAACCTGCGCCTGCCCGGTTGACGCAATCAGTGCTTg                                                               >  2:510348/1‑239 (MQ=255)
                                                                                                                                                                                 tttGCAGGGAAGGGTACATTTGCGCCAGCGTCTGACCGTTGCTCATGGGCAAA‑TCGCCGATCACATTGCGAAAACCGAGG‑ATC‑AAACCGCCGCTAATCAACGCGGGCAGCAACGGGAAGAAGATCACCGCGAAATGAGAGATCaa                                                                                                                                                         >  1:314993/1‑145 (MQ=255)
                                                                                                                                                                                 tttGCAGGGAAGGGTACATTTGCGCCAGCGTCTGACCGTTGCTCATGGGCAAA‑TCGCCGATCACATTGCGAAAACCGAGG‑ATC‑AAACCGCCGCTAATCAACGCGGGCAGCAACGGGAAGAAGATCACCGCGAAATGAGAGATCAACTGCTCATGCCATTTCATATTATGCCGGGCGGCTTTTTTTACCTGCTCTTTATCAACCTGCGCCTGTCCGGTTGACGCAATCAGTGCTTGATAg                                                           >  2:679215/1‑239 (MQ=255)
                                                                                                                                                                                 tttGCAGGGAAGGGTACATTTGCGCCAGCGTCTGACCGTTGCTCATGGGCAAA‑TCGCCGATCACATTGCGAAAACCGAGG‑ATC‑AAACCGCCGCTAATCAACGCGGGCAGCAACGGGAAGAAGATCACCGCGAAATGAGAGATCAACTGCTCATGCCATTTCATATTATGCCGGGCGGCTTTTTTTACCTGCTCTTTATCAACCTGCGCCTGTCCGGTTGACGCAATCAGTGCTTGATAg                                                           >  2:480545/1‑239 (MQ=255)
                                                                                                                                                                                 cttGCAGGGAAGGGTACATTTGCGCCAGCGTCTTACCTTTGCTCATGGGCAAA‑TCGCCGATCACAGAGCGAAAAACGAGG‑ATC‑AAACCGCCGCTAATCAACGCGGACAGCAACGAGAACAATATCACCGCGAAATGAGGGATCAACTGCTCATCCCATTTCATATTATGCCGGGCAGCTTTTTTTAACTGCTCTTTATCAACCTGCGCCTGTCCGGTTGACGCAATCAGTGCTTGTTa                                                            >  2:1105502/2‑238 (MQ=255)
                                                                                                                                                                                   tGCAGGGAAGGGTACATTTGCGCCAGCGTCTGACCGTTGCTCATGGGCAAA‑TCGCCGATCACATTGCGAAAACCGAGG‑ATC‑AAACCGCCGCTAATCAACGCGGGCAGCAACGGGAAGAAGATCACCGCGAAATGAGAGATCAACTGCTCATGCCATTTCATATTATGCCGGGCGGCTTTTTTTACCTGCTCTTTATCAACCTGCGCCTGTCCGGTTGACGCAATCAGTGCTTGCTAGTa                                                         >  2:380754/1‑239 (MQ=255)
                                                                                                                                                                                            gggTACATTTGCGCCAGCGTCTGACCGTTGCTCATGGGCAAA‑TCGCCGATCACATTGCGAAAACCGAGG‑ATC‑AAACCGCCGCTAATCAACGCGGGCAGCAACGGGAAGAAGATCACCGCGAAATGAGAGATCAACTGCTCATGCCATTTCATATTATGCCGGGCGGCTTTTTTTACCTGCTCTTTATCAACCTGCGCCTGTCCGGTTGACGCAATCAGTGCTTGATAGTAATCACCCAc                                                >  2:674859/1‑239 (MQ=255)
                                                                                                                                                                                               tACATTTGCGCCAGCGTCTGACCGTTGCTCATGGGCAAA‑TCGCCGATCACATTGCGAAAACCGAGG‑ATC‑AAACCGCCGCTAATCAACGCGGGCAGCAACGGGAAGAAGATCACCGCGAAATGAGAGATCAACTGCTCATGCCATTTCATATTATGCCGGGCGGCTTTTTTTACCTGCTCTTTATCAAc                                                                                                >  1:346439/1‑188 (MQ=255)
                                                                                                                                                                                               gaCATTTGCGCCATCGTCGCCCCGTTGCTCATGGGCAAA‑TCGCCGACCACATTGCGAAAACCGAGG‑ATC‑AAACCGCCGCTAATCGGCGCGGGCAGCAACGGGAAGAAGATCACCGCGCAATGAGTCATCAACTGCTCATGCGATTTCATATTATGCCGGGCGGCTTTGTTTAAATGCCCTTTATCCACCTGCGCCTGTCCTGTTGACGCACTCAGTGCTCGATCGTAATCACCCACGtt                                             >  2:1055824/2‑239 (MQ=255)
                                                                                                                                                                                                  aTTTGCGCCAGCGTCTGACCGTTGCTCATGGGCAAA‑TCGCCGATCACATTGCGAAAACCGAGG‑ATC‑AAACCGCCGCTAATCAACGCGGGCAGCAACGGGAAGAAGATCACCGCGAAATGAGAGATCAACTGCTCATGCCATTTCATATTATGCCGGGCGGCTTTTTTTACCTGCTCTTTATCAACCTGCGCCTGTCCGGTTGACGCAATCAGTGCTTGATAGTAATCACCCACGTTGGt                                          >  1:1349071/1‑239 (MQ=255)
                                                                                                                                                                                                       cgcCAGCGTCTGACCGTTGCTCATGGGCAAA‑TGGCCGATCACATAGCGAAAACCGAGG‑ATC‑AAACCGCCGCTAATCAACGACGGCAGCAACGGAAAGAAGATCACCGCGAGATGAGAGATCAACTGCTCATGCCATTTCATATTATGCTGGGCGCGTTTTTTTACCTGCTCTTTAACACCCTGAGCCTGTCGGTTTGAAGCTATCAGTGCTTGATATTAGTCACCCAAGttagggacaa                                     >  2:753636/1‑231 (MQ=255)
                                                                                                                                                                                                         ccAGCGTCTGACCGTTGCTCATGGGCAAA‑TCGCCGATCACATTGCGAAAACCGAGG‑ATC‑AAACCGCCGCTAATCAACGCGGGCAGCAACGGGAAGAAGATCACCGCGAAATGAGAGATCAACTGCTCATGCCATTTCATATTATGCCGGGCGGCTTTTTTTACCTGCTCTTTATCAACCTGCGCCTGTCCGGTTGACGCAATCAGTGCTTGATAGTAATCACCCACGTTGGTGCCAATc                                   >  1:1273771/1‑239 (MQ=255)
                                                                                                                                                                                                          cggCGTTTGACCGTTGCCCGAGGGCAAA‑CCCCCGAACAAATGGAGAAACCCAGGG‑AAC‑AAACCGCCGCTAATCAACGCGGGCAGCAACGGGAAGAAGATCACCGCGAAATGAGAGATCAACTGCTCATGCCATTTCATATTATGCCGGGCGGCTTTTTTTACCTGCTCTTTATCAACCTGCGCCTGTCCGGTTGACGCAATCAGTGCTTGATAGTAATCACCCACGTTGGTGCCAATca                                  <  1:1350907/237‑1 (MQ=255)
                                                                                                                                                                                                            gCGTCTGACCGTTGCTCATGGGCAAA‑TCGCCGATCACATTGCGAAAACCGAGG‑ATC‑AAACCGCCGCTAATCAACGCGGGCAGCAACGGGAAGAAGATCACCGCGAAATGAGAGATCAACTGCTCATGCCATTTCATATTATGCCGGGCGGCTTTTTTTACCTGCTCTTTATCAACCTGCGCCTGTCCGGTTGACGCAATCAGTGCTTGATAGTAATCACCCACGTTGGTGCCAATcacc                                >  1:1157459/1‑239 (MQ=255)
                                                                                                                                                                                                               tCTGCCGGTTGCTAAGGGCCAAA‑CCGCCAACCACATGGCAAAAACCGAGA‑AAA‑AAACCGCCGCTAATCAACGCGGGCAGCAACGGGAAGAAGATCACCGCGAAATGAGAGATCAACTGCTCATGCCATTTCATATTATGCCGGGCGGCTTTTTTTACCTGCTCTTTATCAACCTGCGCCTGTCCGGTTGACGCAATCAGTGCTTGATAGTAATCACCCACGTTGGTGCCAATCaccacc                             <  1:1041853/239‑1 (MQ=255)
                                                                                                                                                                                                                   ccggTTGCTCGTGGGCAAA‑CCGCCGACAGCATTGCAAAAACCGAGA‑ATA‑AACCCGCCGCTAATCAACGCGGGCAGCAACGGGAAGAAGATCACCGCGAAATGAGAGATCAACTGCTCATGCCATTTCATATTATGCCGGGCGGCTTTTTTTACCTGCTCTTTATCAACCTGCGCCTGTCCGGTTGACGCAATCAGTGCTTGATAGTAATCACCCACGTTGGTGCCAATCACCACCTGaa                         <  2:1362381/236‑1 (MQ=255)
                                                                                                                                                                                                                        tGCTCATGGGCAAA‑TCGCCGATCACATTGCGAAAACCGAGG‑ATC‑AAACCGCCGCTAATCAACGCGGGCAGCAACGGGAAGAAGATCACCGCGAAATGAGAGATCAACTGCTCATGCCATTTCATATTATGCCGGGCGGCTTTTTTTACCTGCTCTTTATCAACCTGCGCCTGTCCGGTTGACGCAATCAGTGCTTGATAGTAATCACCCACGTTGGTGCCAATCACCACCTGAAATTg                     >  1:554441/1‑238 (MQ=255)
                                                                                                                                                                                                                        tGCTCATGGGCAAA‑TCGCCGATCACATTGCGAAAACCGAGG‑ATC‑AAACCGCCGCTAATCAACGCGGGCAGCAACGGGAAGAAGATCACCGCGAAATGAGAGATCAACTGCTCATGCCATTTCATATTATGCCGGGCGGCTTTTTTTACCTGCTCTTTATCAACCTGCGCCTGTCCGGTTGACGCAATCAGTGCTTGATAGTAATCACCCACGTTGGTGCCAATCACCACCTGAAATTg                     >  1:1178923/1‑238 (MQ=255)
                                                                                                                                                                                                                        tGCTCATGGGCAAA‑TCGCCGATCACATTGCGAAAACCGAGG‑ATC‑AAACCGCCGCTAATCAACGCGGGCAGCAACGGGAAGAAGATCACCGCGAAATGAGAGATCAACTGCTCATGCCATTTCATATTATGCCGGGCGGCTTTTTTTACCTGCTCTTTATCAACCTGCGCCTGTCCGGTTGACGCAATCAGTGCTTGATAGTAATCACCCACGTTGGTGCCAATCACCACCTGAAATTg                     >  1:444413/1‑238 (MQ=255)
                                                                                                                                                                                                                         gCTCATGGGCAAA‑TCGCCGATCACATTGCGAAAACCGAGG‑ATC‑AAACCGCCGCTAATCAACGCGGGCAGCAACGGGAAGAAGATCACCGCGAAATGAGAGATCAACTGCTCATGCCATTTCATATTATGCCGGGCGGCTTTTTTTACCTGCTCTTTATCAACCTGCGCCTGTCCGGTTGACGCAATCAGTGCTTGATAGTAATCACCCACGTTGGTGCCAATCACCACCTGAAATTGc                    >  2:81751/1‑238 (MQ=255)
                                                                                                                                                                                                                          cTCATGGGCAAA‑TCGCCGATCACATTGCGAAAACCGAGG‑ATC‑AAACCGCCGCTAATCAACGCGGGCAGCAACGGGAAGAAGATCACCGCGAAATGAGAGATCAACTGCTCATGCCATTTCATATTATGCCGGGCGGCTTTTTTTACCTGCTCTTTATCAACCTGCGCCTGTCCGGTTGACGCAATCAGTGCTTGCTAGTAATCACCCACGTTGGTGCAAATCACCACCTGAAATTGccc                  >  2:1222484/1‑239 (MQ=255)
                                                                                                                                                                                                                           tCAGGGGCAAA‑CCGCCGATCCCATTGCAAAACCCGAGG‑ACA‑AAACCGCCGCTAATCAACGCGGGCAGCAACGGGAAGAAGATCACCGCGAAATGAGAGATCAACTGCTCATGCCATTTCATATTATGCCGGGCGGCTTTTTTTACCTGCTCTTTATCAACCTGCGCCTGTCCGGTTGACGCAATCAGTGCTTGa                                                              <  2:1141074/194‑1 (MQ=255)
                                                                                                                                                                                                                                  cAAA‑TCGCCGATCACATTGCGAAAACCGAGG‑ATC‑AAACCGCCGCTAATCAACGCGGGCAGCAACGGGAAGAAGATCACCGCGAAATGAGAGATCAACTGCTCATGCCATTTCATATTATGCCGGGCGGCTTTTTTTACCTGCTCTTTATCAACCTGCGCCTGTCCGGTTGACGCAATCAGTGCTTGATAGTAATCACCCACGTTGGTGCCAATCACCACCTGAAATTGCCCGGCATTgg          >  1:335340/1‑239 (MQ=255)
                                                                                                                                                                                                                                     a‑TCGCCGATCACATTGCGAAAACCGAGG‑ATC‑AAACCGCCGCTAATCAACGCGGGCAGCAACGGGAAGAAGATCACCGCGAAATGAGAGATCAACTGCTCATGCCATTTCATATTATGCCGGGCGGCTTTTTTTACCTGCTCTTTATCAACCTGCGCCTGTCCGGTTGACGCAATCAGTGCTTGATAGTAATCAc                                                    >  1:1141074/1‑194 (MQ=255)
                                                                                                                                                                                                                                         ccctaTCACATGGCAAAACCCGAGA‑ATA‑AAACCGCCGCTAATCAACGCGGGCAGCAACGGGAAGAAGATCACCGCGAAATGAGAGATCAACTGCTCATGCCATTTCATATTATGCCGGGCGGCTTTTTTTACCTGCTCTTTATCAACCTGCGCCTGTCCGGTTGACGCAATCAGTGCTTGATAGTAATCACCCACGTTGGTGCCAATCACCACCTGAAATTGCCCGGCATTGGTGAAAc    <  1:595532/235‑1 (MQ=255)
                                                                                                                                                                                                                                             aTCACATTGCGAAAACCGAGG‑ATC‑AAACCGCCGCTAATCAACGCGGGCAGCAACGGGAAGAAGATCACCGCGAAATGAGAGATCAACTGCTCATGCCATTTCATATTATGCCGGGCGGCTTTTTTTACCTGCTCTTTATCAACCTGCGCCTGTCCGGTTGACGCAATCAGTGCTTGATAGTAATCACCCACGTTGGTGCCAATCACCACCTGAAATTGCCCGGCATTGGTGAAACAg  >  1:1056941/1‑237 (MQ=255)
                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                             
CCGGCAGCTGCTGCCCGAGCAGATAAGCGTTCATCAGCTGTGGAGAAACCAGTGTCACGCCAAGCACGATACCAAGGATCGGCGTGCCGCCCATTTTTTTCACCGCTGACCAGCAAATACCGACCGGCAGGTAGAAGAAGATCGCTTCACCGATCAACCACAGAAAATCGTAGATCGTTTGCAGGGAAGGGTACATTTGCGCCAGCGTCTGACCGTTGCTCATGGGCAAA‑TCGCCGATCACATTGCGAAAACCGAGG‑ATC‑AAACCGCCGCTAATCAACGCGGGCAGCAACGGGAAGAAGATCACCGCGAAATGAGAGATCAACTGCTCATGCCATTTCATATTATGCCGGGCGGCTTTTTTTACCTGCTCTTTATCAACCTGCGCCTGTCCGGTTGACGCAATCAGTGCTTGATAGTAATCACCCACGTTGGTGCCAATCACCACCTGAAATTGCCCGGCATTGGTGAAACAG  >  NC_000913/4465541‑4466013

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑