Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,004,945 G→A 6.9% V28V (GTG→GTA)  pldA → outer membrane phospholipase A

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,004,9450GA 6.9% 180.3 / 4.4 58V28V (GTG→GTA) pldAouter membrane phospholipase A
Reads supporting (aligned to +/- strand):  ref base G (34/20);  new base A (2/2);  total (36/22)
Fisher's exact test for biased strand distribution p-value = 6.30e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.92e-01

GGCTTGTTCAGCGGTCAGTACGGCAGACATATCATCCTTACACTTCATTGGTTAATGAAATGTTGATTTTATGCTTCTTTGTTGTTGGTTTCTACTTTAGGAAGGGATAACTAACGGCTATGGAGTTAAGTATGTAAAATAGCCCGCAGAAAAATATTCACCTTATCAATAATTCGTTACGGAGAACACGACCGATGCGGACTCTGCAGGGCTGGTTGTTGCCGGTGTTTATGTTGCCTATGGCAGTATATGCACAAGAGGCAACGGTGAAAGAGGTGCATGACGCGCCAGCGGTGCGTGGCAGTATTATCGCCAATATGCTGCAGGAGCATGACAATCCGTTCACGCTCTATCCTTATGACACCAACTACCTCATTTACACCCAAACCAGCGATCTGAATAAAGAAGCGATTGCCAGTTACGACTGGGCGGAAAATGCGCGTAAGGATGAAGTAAAGTTTCAGTTGAGCCTGGCATTTCCGCTGTGGCGTGGGATTTTAGGCCCGAACTCGGTGTTGGGTGCGTCTTATACGCAAAAATCCTGGTGGCAACTGT  >  NC_000913/4004668‑4005222
                                                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                                                     
ggCTTGTTCAGCGGTCAGTACGGCAGACATATCATCCTTACACTTCATTGGTTAATGAAATGTTGATTTTATGCTTCTTTGTTGTTGGTTTCTACTTTAGGAAGGGATAACTAACGGCTATGGAGTTAAGTATGTAAAATAGCCCGCAGAAAAATATTCACCTTATCAATAATTCGTTACGGAGAACACGACCGATGCGGACTCTGCAGGGCTGGTTGTTGCCGGTGTTTATGTTGCCTATGGCAGTATATGCACAAGAGGCAACGGTGAAAGAGGTGCATGACgcgc                                                                                                                                                                                                                                                                             >  2:280649/1‑288 (MQ=255)
     gTTCAGCGGTCAGTACGGCAGACATATCATCCTTACACTTCATTGGTTAATGAAATGTTGATTTTATGCTTCTTTGTTGTTGGTTTCTACTTTAGGAAGGGATAACTAACGGCTATGGAGTTAAGTATGTAAAATAGCCCGCAGAAAAATATTCACCTTATCAATAATTCGTTACGGAGAACACGACCGATGCGGACTCTGCAGGGCTGGTTGTTGCCGGTGTTTATGTTGCCTATGGCAGTATATGCACAAGAGGCAACGGTGAAAGAGGTGCATGACGCGCCAGCgg                                                                                                                                                                                                                                                                       >  1:688311/1‑289 (MQ=255)
       tCAGCGGTCAGTACGGCAGACATATCATCCTTACACTTCATTGGTTAATGAAATGTTGATTTTATGCTTCTTTGTTGTTGGTTTCTACTTTAGGAAGGGATAACTAACGGCTATGGAGTTAAGTATGTAAAATAGCCCGCAGAAAAATATTCACCTTATCAATAATTCGTTACGGAGAACACGACCGATGCGGACTCTGCAGGGCTGGTTGTTGCCGGTGTTTATGTTGCCTATGGCAGTATATGCACAAGAGGCAACGGTGAAAGAGGTGCATGACGCGCCAGCGGTg                                                                                                                                                                                                                                                                     <  1:574653/289‑1 (MQ=255)
                aGTACGGCAGACATATCATCCTTACACTTCATTGGTTAATGAAATGTTGATTTTATGCTTCTTTGTTGTTGGTTTCTACTTTAGGAAGGGATAACTAACGGCTCTGGCGTTAAGTATGTAAAATAGCCCGCAGAAAAATATTCACCTTATCAATAGTTCGTTACGGAGAACACGACCGATGCGGCCTCTGCAGGGGTGGTTGTTGCCGGTGTTTATGTTGCCTATGGCGGTCTATGCACAAGCGGCAACGGTGAAAGCGGTGCATGACGCGCAAGAGGTGCGTGGCAGt                                                                                                                                                                                                                                                            >  2:61713/1‑289 (MQ=255)
                           cATATCATCCTTACACTTCATTGGTTAATGAAATGTTGATTTTATGCTTCTTTGTTGTTGGTTTCTACTTTAGGAAGGGATAACTAACGGCTATGGAGTTAAGTATGTAAAATAGCCCGCAGAAAAATATTCACCTTATCAATAATTCGTTACGGAGAACACGACCGATGCGGACTCTGCAGGGCTGGTTGTTGCCGGTGTTTATGTTGCCTATGGCAGTATATGCACAAGAGGCAACGGTGAAAGAGGTGCATGACGCGCCAGCGGTGCGTGGCAGTATTATCGCCaa                                                                                                                                                                                                                                                 <  1:609524/289‑1 (MQ=255)
                                 atcCTTACACTTCATTGGTTAATGAAATGTTGATTTTATGCTTCTTTGTTGTTGGTTTCTACTTTAGGAAGGGATAACTAACGGCTATGGAGTTAAGTATGTAAAATAGCCCGCAGAAAAATATTCACCTTATCAATAATTCGTTACGGAGAACACGACCGATGCGGACTCTGCAGGGCTGGTTGTTGCCGGTGTTTATGTTGCCTATGGCAGTATATGCACAAGAGGCAACGGTGAAAGAGGTGCATGAcg                                                                                                                                                                                                                                                                                >  1:142358/1‑252 (MQ=255)
                                 atcCTTACACTTCATTGGTTAATGAAATGTTGATTTTATGCTTCTTTGTTGTTGGTTTCTACTTTAGGAAGGGATAACTAACGGCTATGGAGTTAAGTATGTAAAATAGCCCGCAGAAAAATATTCACCTTATCAATAATTCGTTACGGAGAACACGACCGATGCGGACTCTGCAGGGCTGGTTGTTGCCGGTGTTTATGTTGCCTATGGCAGTATATGCACAAGAGGCAACGGTGAAAGAGGTGCATGAcg                                                                                                                                                                                                                                                                                <  2:142358/252‑1 (MQ=255)
                                   ccTTACACTTCATTGGTTAATGAAATGTTGATTTTATGCTTCTTTGTTGTTGGTTTCTACTTTAGGAAGGGATAACTAACGGCTATGGAGTTAAGTATGTAAAATAGCCCGCAGAAAAATATTCACCTTATCAATAGTTCGTTACGGAGAACACGACCGATGCGGACTCTGCAGGGCTGGTTGTTGCCGGTGTTTATGTTGCCTATGGCAGTATATGCACAAGAGGCAACGGTGAAAGAGGTGCATGACGCGCCAGCGGTGCGTGGCAGTATTATCGCCAATAtgctgc                                                                                                                                                                                                                                         <  1:61713/289‑1 (MQ=255)
                                      tACACTTCATTGGTTAATGAAATGTTGATTTTATGCTTCTTTGTTGTTGGTTTCTACTTTAGGAAGGGATAACTAACGGCTATGGAGTTAAGTATGTAAAATAGCCCGCAGAAAAATATTCACCTTATCAATAATTCGTTACGGAGAACACGACCGATGCGGACTCTGCAGGGCTGGTTGTTGCCGGTGTTTATGTTGCCTATGGCAGTATATGCACAAGAGGCAACGGTGAAAGAGGTGCATGACGCGCCAGCGGTGCGTGGCAGTATTATCGCCAata                                                                                                                                                                                                                                               <  2:342934/280‑1 (MQ=255)
                                      tACACTTCATTGGTTAATGAAATGTTGATTTTATGCTTCTTTGTTGTTGGTTTCTACTTTAGGAAGGGATAACTAACGGCTATGGAGTTAAGTATGTAAAATAGCCCGCAGAAAAATATTCACCTTATCAATAATTCGTTACGGAGAACACGACCGATGCGGACTCTGCAGGGCTGGTTGTTGCCGGTGTTTATGTTGCCTATGGCAGTATATGCACAAGAGGCAACGGTGAAAGAGGTGCATGACGCGCCAGCGGTGCGTGGCAGTATTATCGCCAata                                                                                                                                                                                                                                               >  1:342934/1‑280 (MQ=255)
                                      tACACTTCATTGGTTAATGAAATGTTGATTTTATGCTTCTTTGTTGTTGGTTTCTACTTTAGGAAGGGATAACTAACGGCTATGGAGTTAAGTATGTAAAATAGCCCGCAGAAAAATATTCACCTTATCAATAATTCGTTACGGAGAACACGACCGATGCGGACTCTGCAGGGCTGGTTGTTGCCGGTGTTTATGTTGCCTATGGCAGTATATGCACAAGAGGCAACGGTGAAAGAGGTACATGACGCGCCAGCGGTGCGTGGCAGTATTATCGCCAATATGCTGCAgg                                                                                                                                                                                                                                      >  1:126434/1‑289 (MQ=255)
                                      tACACTTCATTGGTTAATGAAATGTTGATTTTATGCTTCTTTGTTGTTGGTTTCTACTTTAGGAAGGGATAACTAACGGCTATGGAGTTAAGTATGTAAAATAGCCCGCAGAAAAATATTCACCTTATCAATAATTCGTTACGGAGAACACGACCGATGCGGACTCTGCAGGGCTGGTTGTTGCCGGTGTTTATGTTGCCTATGGCAGTATATGCACAAGAGGCAACGGTGAAAGAGGTACATGACGCGCCAGCGGTGCGTGGCAGTATTATCGCCAATATGCTGCAgg                                                                                                                                                                                                                                      >  1:126443/1‑289 (MQ=255)
                                            tCATTGGTTAATGAAATGTTGATTTTATGCTTCTTTGTTGTTGGTTTCTACTTTAGGAAGGGATAACTAACGGCTATGGAGTTAAGTATGTAAAATAGCCCGCAGAAAAATATTCACCTTATCAATAATTCGTTACGGAGAACACGACCGATGCGGACTCTGCAGGGCTGATTGTTGCCGGTGTTTATGTTGCCTATGGCAGTATATGCACAATAGGCAACGGCGAAAGAGGTGCATGATGCGCCAGCGGTTCGTGGCAGTCTTATCGCCCATATGCTGCAGGCGCGTg                                                                                                                                                                                                                                >  2:541025/1‑289 (MQ=255)
                                             cATTGGTTAATGAAATGTTGATTTTATGCTTCTTTGTTGTTGGTTTCTACTTTAGGAAGGGATAACTAACGGCTATGGAGTTAAGTATGTAAAATAGCCCGCAGAAAAATATTCACCTTATCAATAATTCGTTACGGAGAACACGACCGATGCGGACTCTGCAGGGCTGGTTGTTGCCGGTGTTTATGTTGCCTATGGCAGTATATGCACAAGAGGCAACGGTGAAAGAGGTGCATGACGCGCCAGCGGTGCg                                                                                                                                                                                                                                                                   >  2:130987/1‑253 (MQ=255)
                                             cATTGGTTAATGAAATGTTGATTTTATGCTTCTTTGTTGTTGGTTTCTACTTTAGGAAGGGATAACTAACGGCTATGGAGTTAAGTATGTAAAATAGCCCGCAGAAAAATATTCACCTTATCAATAATTCGTTACGGAGAACACGACCGATGCGGACTCTGCAGGGCTGGTTGTTGCCGGTGTTTATGTTGCCTATGGCAGTATATGCACAAGAGGCAACGGTGAAAGAGGTGCATGACGCGCCAGCGGTGCg                                                                                                                                                                                                                                                                   <  1:130987/253‑1 (MQ=255)
                                             cATTGGTTAATGAAATGTTGATTTTATGCTTCTTTGTTGTTGGTTTCTACTTTAGGAAGGGATAACTAACGGCTATGGAGTTAAGTATGTAAAATAGCCCGCAGAAAAATATTCACCTTATCAATAATTCGTTACGGAGAACACGACCGATGCGGACTCTGCAGGGCTGGTTGTTGCCGGTGTTTATGTTGCCTATGGCAGTATATGCACAAGAGGCAACGATGAAAGAGGTGCATGACGCGCCAGCGGTCCGTGGCAGTATTATCGCCAATATGCTGCAGGCGCAtt                                                                                                                                                                                                                                >  1:216688/1‑287 (MQ=255)
                                                                                                ttAGGAAGGGATAACTAACGGCTATGGAGTTAAGTATGTAAAATAGCCCGCAGAAAAATATTCACCTTATCAATAATTCGTTACGGAGAACACGACCGATGCGGACTCTGCAGGGCTGGTTGTTGCCGGTGTTTATGTTGCCTATGGCAGTATATGCACAAGAGGCAACGGTGAAAGAGGTGCATGACGCGCCAGCGGTGCGTGGCAGTATTATCGCCAATATGCTGCAGGAGCATGACAATCCGTTCACGCTCTATCCTTATGACACCAACTACCTCATTTACACCCa                                                                                                                                                                            <  1:280649/289‑1 (MQ=255)
                                                                                                        ggATAACTAACGGCTATGGAGTTAAGTATGTAAAATAGCCCGCAGAAAAATATTCACCTTATCAATAATTCGTTACGGAGAACACGACCGATGCGGACTCTGCAGGGCTGGTTGTTGCCGGTGTTTATGTTGCCTATGGCAGTATATGCACAAGAGGCAACGGTGAAAGAGGTGCATGACGCGCCAGCGGTGCGTGGCAGTATTATCGCCAATATGCTGCAGGAGCATGACCATCCGTTCACGCCCTATCCTTCTGACACCAACTACCTCATTTACACCCAAACCAGcc                                                                                                                                                                    >  2:540783/1‑288 (MQ=255)
                                                                                                                  cGGCTATGGAGTTAAGTATGTAAAATAGCCCGCAGAAAAATATTCACCTTATCAATAATTCGTTACGGAGAACACGACCGATGCGGACTCTGCAGGGCTGGTTGTTGCCGGTGTTTATGTTGCCTATGGCAGTATATGCACAAGAGGCAACGGTGAAAGAGGTGCATGACGCGCCAGCGGTGCGTGGCAGTATTATCGCCAATATGCTGCAGGAGCCTGACAATCAGTTTACGCGCTATCCTTATTAAACCAACTACCTCATTTACACCCCAACCAGCGCTCTCAATaa                                                                                                                                                          >  1:40011/1‑289 (MQ=255)
                                                                                                                   ggCTATGGAGTTAAGTATGTAAAATAGCCCGCAGAAAAATATTCACCTTATCAATAATTCGTTACGGAGAACACGACCGATGCGGACTCTGCAGGGCTGGTTGTTGCCGGTGTTTATGTTGCCTATGGCAGTATATGCACAAGAGGCAACGGTGAAAGAGGTGCATGACGCGCCAGCGGTGCGTGGCAGTATTATCGCCAATATGCTGCAGGAGCATGACAATCCGTTCACGCTCTATCCTTATGACACCAACTACCTCATTTACACCCAAACCAGCGATCTGAATaaa                                                                                                                                                         >  1:574037/1‑289 (MQ=255)
                                                                                                                        tGGAGTTAAGTATGTAAAATAGCCCGCAGAAAAATATTCACCTTATCAATAATTCGTTACGGAGAACACGACCGATGCGGACTCTGCAGGGCTGGTTGTTGCCGGTGTTTATGTTGCCTATGGCAGTATATGCACAAGAGGCAACGGTGAAAGAGGTGCATGACGCGCCAGCGGTGCGTGGCAGTATTATCGCCAATATGCTGCAGGAGCATGACAATCCGTTCACGCTCTATCCTTATGACACCAACTACCTCATTTACACCCAAACCAGCGATCTGAATAAAGAAGc                                                                                                                                                    >  2:675138/1‑289 (MQ=255)
                                                                                                                                     gTAAAATAGCCCGCAGAAAAATATTCACCTTATCAATAATTCGTTACGGAGAACACGACCGATGCGGACTCTGCAGGGCTGGTTGTTGCCGGTGTTTATGTTGCCTATGGCAGTATATGCACAAGAGGCAACGGTGAAAGAGGTGCATGACGCGCCAGCGGTGCGTGGCAGTATTATCGCCAATATGCTGCAGGAGCATGACAATCCGTTCACGCTCTATCCTTATGAACCCAACTACCTCATTTAAACCCCAACAAGCGACCTGCATCAAGAATCGATTACCAggt                                                                                                                                         >  2:58053/1‑285 (MQ=255)
                                                                                                                                                  cAGAAAAATATTCACCTTATCAATAATTCGTTACGGAGAACACGACCGATGCGGACTCTGCAGGGCTGGTTGTTGCCGGTGTTTATGTTGCCTATGGCAGTATATGCACAAGAGGCAACGGTGAAAGAGGTGCATGACGCGCCAGCGGTGCGTGGCAGTATTATCGCCAATATGCTGCAGGAGCATGACAATCCGTTCACGCTCTATCCTTATGACACCAACTACCTCATTTACACCCAAACCAGCGATCTGAATAAAGAAGCGATTGCCAGTTACGACTGGGCGGaaa                                                                                                                          >  1:322193/1‑289 (MQ=255)
                                                                                                                                                    aaaaaaTATTCACCGTATCAATAATTCGTGACGGAGAACACGACCGATGCGGACTCTGCAGGGCTGGGTGTTGCCGGTGGTTATGTTGCCTATGGCAGTATATGCACAAGAGGCAACGGTGAAAGAGGTGCATGACGCGCCAGCGGTGCGTGGCAGTATTATCGCCAATATGCTGCAGGAGCATGACAATCCGTTCACGCTCTATCCTTATGACACCAACTACCTCATTTACACCCAAACCAGCGATCTGAATAAAGAAGCGATTGCCAGTTACGACTGGGCGGAAAAt                                                                                                                        <  2:690918/288‑1 (MQ=255)
                                                                                                                                                                           atTCGTTACGGAGAACACGACCGATGCGGACTCTGCAGGGCTGGTTGTTGCCGGTGTTTATGTTGCCTATGGCAGTATATGCACAAGAGGCAACGGTGAAAGAGGTGCATGACGCGCCAGCGGTGCGTGGCAGTATTATCGCCAATATGCTGCAGGAGCATGACAATCCGTTCACGCTCTATCCTTATGACACCAACTACCTCATTTACACCCAAACCAGCGATCTGAATAAAGAAGCGATTGCCAGTTACGACTGGGCGGa                                                                                                                            <  1:238397/262‑1 (MQ=255)
                                                                                                                                                                           atTCGTTACGGAGAACACGACCGATGCGGACTCTGCAGGGCTGGTTGTTGCCGGTGTTTATGTTGCCTATGGCAGTATATGCACAAGAGGCAACGGTGAAAGAGGTGCATGACGCGCCAGCGGTGCGTGGCAGTATTATCGCCAATATGCTGCAGGAGCATGACAATCCGTTCACGCTCTATCCTTATGACACCAACTACCTCATTTACACCCAAACCAGCGATCTGAATAAAGAAGCGATTGCCAGTTACGACTGGGCGGa                                                                                                                            >  2:238397/1‑262 (MQ=255)
                                                                                                                                                                           atTCGTTACGGAGAACACGACCGATGCGGACTCTGCAGGGCTGGTTGTTGCCGGTGTTTATGTTGCCTATGGCAGTATATGCACAAGAGGCAACGGTGAAAGAGGTGCATGACGCGCCAGCGGTGCGTGGCAGTATTATCGCCAATATGCTGCAGGAGCATGACAATCCGTTCACGCTCTATCCTTATGACACCAACTACCTCATTTACACCCAAACCAGCGATCTGAATAAAGAAGCGATTGCCAGTTACGACTGGGCGGAAAATGCGCGTAAGGATGAAGTAAAGtt                                                                                                 >  1:611312/1‑289 (MQ=255)
                                                                                                                                                                                 tACGGAGACAACTACCGATGGTGAGTCTGGAGGGCTGGTTGTTGCCGGTGTTTATGTTGCCTATGGCAGTATATGGACAAGAGGCAACGGTGAAAGAGGTGCATGACGCGCCAGCGGTGCGTGGCAGTAGTAGCGCCAATATGCTGCAGGAGCATGACAATCCGTTCACGCTCTATCCGTATGACACGAACTACCTCATTTACACCAAAACCAGCGATCTGAATAAAGAAGCGATTGCCAGTTACGACTGGGCGGAAAATGCGCGTAAGGATGAAGTAAAGTTTCAGt                                                                                            <  2:216688/288‑1 (MQ=255)
                                                                                                                                                                                        aaCACGACCGATGCGGACTCTGCAGGGCTGGTTGTTGCCGGTGTTTATGTTGCCTATGGCAGTATATGCACAAGAGGCAACGGTGAAAGAGGTGCATGACGCGCCAGCGGTGCGAGGCAGTATTATCGCCAATATGCTGCAGGAGCATGACAATCCGTTCACGCTCTATCCTTATGACACCAACTACCTAATTTACACCCAAACCAGCGATCTGAATAAAGAATCGATTGCCAGTTACGACTGGGCGGAAAATGAGAGTAAGGCTGAAGTAAAGCTTCAGtttcacctg                                                                                    >  2:427377/1‑282 (MQ=255)
                                                                                                                                                                                                  aTGCGGACTCTGCAGGGCTGGTTGTTGCCGGTGTTTATGTTGCCTATGGCAGTATATGCACAAGAGGCAACGGTGAAAGAGGTGCATGACGCGCCAGCGGTGCGTGGCAGTATTATCGCCAATATGCTGCAGGAGCATGACAATCCGTTCACGCTCTATCCTTATGACACCAACTACCTCATTTACACCCAAACCAGCGCTCTGAATAAAGAAGCGATTGCCAGTTACGACTGGGCGGAAAATGCGCGTAAGGATGAAGTAAAGTTTCAGTTGAGCCTGGCATTTCCg                                                                           >  1:669560/1‑288 (MQ=255)
                                                                                                                                                                                                  aTGCGGACTCTGCAGGGCTGGTTGTTGCCGGTGTTTATGTTGCCTATGGCAGTATATGCACAAGAGGCAACGGTGAAAGAGGTGCATGACGCGCCAGCGGTGCGTGGCAGTATTATCGCCAATATGCTGCAGGAGCATGACAATCCGTTCACGCTCTATCCTTATGACACCAACTACCTCATTTACACCCAAACCAGCGATCTGAATAAAGAAGCGATTGCCAGTTACGACTGGGCGGAAAATGCGCGTAAGGATGAAGTAAAGTTTCAGTTGAGCCTGGCATTTcc                                                                            >  1:668459/1‑287 (MQ=255)
                                                                                                                                                                                                  aTGCGGACTCTGCAGGGCTGGTTGTTGCCGGTGTTTATGTTGCCTATGGCAGTATATGCACAAGAGGCAACGGTGAAAGAGGTGCATGACGCGCCAGCGGTGCGTGGCAGTATTATCGCCAATATGCTGCAGGAGCATGACAATCCGTTCACGCTCTATCCTTATGACACCAACTACCTCATTTACACCCAAACCAGCGATCTGAATAAAGAAGCGATTGCCAGTTACGACTGGGCGGAAAATGCGCGTAAGGATGAAGTAAAGTTTCAGTTGAGCCTGGCATTTCCg                                                                           >  2:699091/1‑288 (MQ=255)
                                                                                                                                                                                                    gCGGACTCTGCAGGGCTGGTTGTTGCCGGTGTTTATGTTGCCTATGGCAGTATATGCACAAGAGGCAACGGTGAAAGAGGTGCATGACGCGCCAGCGGTGCGTGGCAGTATTATCGCCAATATGCTGCAGGAGCATGACAATCCGTTCACGCTCTATCCTTATGACACCAACTACCTCATTTACACCCAAACCAGCGATCTGAATAAAGAAGCGATTGCCAGTTACGACTGGGCGGAAAATGCGCGTAAGGATGAAGTAAAGTTTCAGTTGAGCCTGGCATTTCCGCt                                                                         <  2:574037/288‑1 (MQ=255)
                                                                                                                                                                                                          tctGCAGGGCTGGTTGTTGCCGGTGTTTATGTTGCCTATGGCAGTATATGCACAAGAGGCAACGGTGAAAGAGGTGCATGACGCGCCAGCGGTGCGTGGCAGTATTATCGCCAATATGCTGCAGGAGCATGACAATCCGTTCACGCTCTATCCTTATGACACCAACTACCTCATTTACACCCAAACCAGCGATCTGAATAAAGAAGCGATTGCCAGTTACGACTGGGCGGAAAATGCGCGTAAGGATGAAGTAAAGTTTCAGTTGAGCCTGGCATTTCCGCTGTGGCGt                                                                  <  1:540783/289‑1 (MQ=255)
                                                                                                                                                                                                                  gCTGGTTGTTGCCGGTGTTTATGTTGCCTATGGCAGTATATGCACAAGAGGCAACGGTGAAAGAGGTGCATGACGCGCCAGCGGTGCGTGGCAGTATTATCGCCAATATGCTGCAGGAGCATGACAATCCGTTCACGCTCTATCCTTATGACACCAACTACCTCATTTACACCCAAACCAGCGATCTGAATAAAGAAGCGATTGCCAGTTACGACTGGGCGGAAAATGCGCGTAAGGATGAAGTAAAGTTTCAGTTGAGCCTGGCATTTCCGCTGTGGCGTGGGAttt                                                           <  1:675138/288‑1 (MQ=255)
                                                                                                                                                                                                                      gttgttGCCGGTGTTTATGTTGCCTATGGCAGTATATGCACAAGAGGCAACGGTGAAAGAGGTGCATGACGCGCCAGCGGTGCGTGGCAGTATTATCGCCAATATGCTGCAGGAGCATGACAATCCGTTCACGCTCTATCCTTATGACACCAACTACCTCATTTACACCCAAACCAGCGATCTGAATAAAGAAGCGATTGCCAGTTACGACTGGGCGGAAAATGCGCGTAAGGATGAAGTAAAGTTTCAGTTGAGCCTGGCATTTCCGCTGTGGCGTGGGATTTTAgg                                                       >  1:113196/1‑288 (MQ=255)
                                                                                                                                                                                                                       ttgttgCCGGTGTTTATGTTGCCTATGGCAGTATATGCACAAGAGGCAACGGTGAAAGAGGTACATGACGCGCCAGCGGTGCGTGGCAGTATTATCGCCAATATGCTGCAGGAGCATGACAATCCGTTCACGCTCTATCCTTATGACACCAACTACCTCATTTACACCCAAACCAGCGATCTGAATAAAGAAGCGATTGCCAGTTACGACTGGGCGGAAAATGCGCGTAAGGATGAAGTAAAGTTTCAGTTGAGCCTGGCATTTCCGCTGTGGCGTGGGATTTTAGGc                                                      <  2:126434/288‑1 (MQ=255)
                                                                                                                                                                                                                       ttgttgCCGGTGTTTATGTTGCCTATGGCAGTATATGCACAAGAGGCAACGGTGAAAGAGGTACATGACGCGCCAGCGGTGCGTGGCAGTATTATCGCCAATATGCTGCAGGAGCATGACAATCCGTTCACGCTCTATCCTTATGACACCAACTACCTCATTTACACCCAAACCAGCGATCTGAATAAAGAAGCGATTGCCAGTTACGACTGGGCGGAAAATGCGCGTAAGGATGAAGTAAAGTTTCAGTTGAGCCTGGCATTTCCGCTGTGGCGTGGGATTTTAGGc                                                      <  2:126443/288‑1 (MQ=255)
                                                                                                                                                                                                                            gCCGGTGTTTATGTTGCCTATGGCAGTATATGCACAAGAGGCAACGGTGAAAGAGGTGCATGACGCGCCAGCGGTGCGTGGCAGTATTATCGCCAATATGCTGCAGGAGCATGACAATCCGTTCACGCTCTATCCTTATGACACCAACTACCTCATTTACACCCAAACCAGCGATCTGAATAAAGAAGCGATTGCCAGTTACGACTGGGCGGAAAATGCGCGTAAGGATGAAGTAAAGTTTCAGTTGAGCCTGGCATTTCCGCTGTGGCGTGGGATTTTAGGCCCGa                                                  >  1:657283/1‑287 (MQ=255)
                                                                                                                                                                                                                                           gCCTATGGCAGTATATGCACAAGAGGCAACGGTGAAAGAGGTGCATGACGCGCCAGCGGTGCGTGGCAGTATTATCGCCAATATGCTGCAGGAGCATGACAATCCGTTCACGct                                                                                                                                                                                                                >  1:437553/1‑114 (MQ=255)
                                                                                                                                                                                                                                           gCCTATGGCAGTATATGCACAAGAGGCAACGGTGAAAGAGGTGCATGACGCGCCAGCGGTGCGTGGCAGTATTATCGCCAATATGCTGCAGGAGCATGACAATCCGTTCACGct                                                                                                                                                                                                                <  2:437553/114‑1 (MQ=255)
                                                                                                                                                                                                                                           gCCTATGGCAGTATATGCACAAGAGGCAACGGTGAAAGAGGTGCATGACGCGCCAGCGGTGCGTGGCAGTATTATCGCCAATATGCTGCAGGAGCATGACAATCCGTTCACGCTCTATCCTTATGACACCAACTACCTCATTTACACCCAAACCAGCGATCTGAATAAAGAAGCGATTGCCAGTTACGACTGGGCGGAAAATGCGCGTAAGGATGAAGTAAAGTTTCAGTTGAGCCTGGCATTTCCGCTGTGGCGTGGGATTTTAGGCCCGAACTCGGTGTTGGGTGCg                                 >  2:345050/1‑289 (MQ=255)
                                                                                                                                                                                                                                            ccTATGGCAGTATATGCACAAGAGGCAACGGTGAAAGAGGTGCATGACGCGCCAGCGGTGCGTGGCAGTATTATCGCCAATATGCTGCAGGAGCATGACAATCCGTTCACGCTCTATCCTTATGACACCAACTACCTCATTTACACCCAAACCAGCGATCTGAATAAAGAAGCGATTGCCAGTTACGACTGGGCGGAAAATGCGCGTAAGGATGAAGTAAAGTTTCAGTTGAGCCTGGCATTTCCGCTGTGGCGTGGGATTTTAGGCCCGAACTCGGTGTTGGGTGCg                                 >  1:2163/1‑288 (MQ=255)
                                                                                                                                                                                                                                                      tataTGCACAAGAGGCAACGGTGAAAGAGGTGCATGACGCGCCAGCGGTGCGTGGCAGTATTATCGCCAATATGCTGCAGGAGCATGACAATCCGTTCACGCTCTATCCTTATGACACCAACTACCTCATTTACACCCAAACCAGCGATCTGAATAAAGAAGCGATTGCCAGTTACGACTGGGCGGAAAATGCGCGTAAGGATGAAGTAAAGTTTCAGTTGAGCCTGGCATTTCCGCTGTGGCGTGGGATTTTAGGCCCGAACTCGGTGTTGGGTGCGTCTTATACGCa                      >  1:246721/1‑289 (MQ=255)
                                                                                                                                                                                                                                                                agagGCAACGGTGAAAGAGGTGCATGACGCGCCAGCGGTGCGTGGCAGTATTATCGCCAATATGCTGCAGGAGCATGACAATCCGTTCACGCTCTATCCTTATGACACCAACTACCTCATTTACACCCAAACCAGCGATCTGAATAAAGAAGCGATTGCCAGTTACGACTGGGCGGAAAATGCGCGTAAGGATGAAGTAAAGTTTCAGTTGAGCCTGGCATTTCCGCTGTGGCGTGGGATTTTAGGCCCGAACTCGGTGTTGGGTGCGTCTTATACGCAAAAATCCtgg            >  2:658422/1‑289 (MQ=255)
                                                                                                                                                                                                                                                                agagGCAACGGTGAAAGAGGTGCATGACGCGCCAGCGGTGCGTGGCAGTATTATCGCCAATATGCTGCAGGAGCATGACAATCCGTTCACGCTCTATCCTTATGACACCAACTACCTCATTTACACCCAAACCAGCGATCTGAATAAAGAAGCGATTGCCAGTTACGACTGGGCGGAAAATGCGCGTAAGGATGAAGTAAAGTTTCAGTTGAGCCTGGCATTTCCGCTGTGGCGTGGGATTTTAGGCCCGAACTCGGTGTTGGGTGCGTCTTATACGCAAAAATCCtgg            >  2:656508/1‑289 (MQ=255)
                                                                                                                                                                                                                                                                agagGCAACGGTGAAAGAGGTGCATGACGCGCCAGCGGTGCGTGGCAGTATTATCGCCAATATGCTGCAGGAGCATGACAATCCGTTCACGCTCTATCCTTATGACACCAACTACCTCATTTACACCCAAACCAGCGATCTGAATAAAGAAGCGATTGCCAGTTACGACTGGGCGGAAAATGCGCGTAAGGATGAAGTAAAGTTTCAGTTGAGCCTGGCATTTCCGCCGTGGCGTGGGATTTTAGGCCCGAACTCGGTGTTGGGTTCGTCTTTTACGGAAAAATCCggg            >  2:238895/1‑289 (MQ=255)
                                                                                                                                                                                                                                                                 gagGCAACGGTGAAAGAGGTGCATGACGCGCCAGCGGTGCGTGGCAGTATTATCGCCAATATGCTGCAGGAGCATGACAATCCGTTCACGCTCTATCCTTATGACACCAACTACCTCATTTACACCCAAACCAGCGATCTGAATAAAGAAGCGATTGCCAGTTACGACTGGGCGGAAAATGCGCGTAAGGATGAAGTAAAGTTTCAGTTGAGCCTGGCATTTCCGCTGTGGCGTGGGATTTTAGGCCCGAACTCGGTGTTGGGTGCGTCTTATACGCAAAAATCCtggt           >  1:259274/1‑289 (MQ=255)
                                                                                                                                                                                                                                                                     cAACGGTGAAAGAGGTGCATGACGCGCCAGCGGTGCGTGGCAGTATTATCGCCAATATGCTGCAGGAGCATGACAATCCGTTCACGCTCTATCCTTATGACACCAACTACCTCATTTACACCCAAACCAGCGATCTGAATAAAGAAGCGATTGCCAGTTACGACTGGGCGGAAAATGCGCGTAAGGATGAAGTAAAGTTTCAGTTGAGCCTGGCATTTCCGCTGTGGCGTGGGATTTTAGGCCCGAACTCGGTGTTGGGTGCGTCTTATACGCAAAAATCCTGGTGGc        <  1:345050/288‑1 (MQ=255)
                                                                                                                                                                                                                                                                     cAACGGTGAAAGAGGTGCATGACGCGCCAGCGGTGCGTGGCAGTATTATCGCCAATATGCTGCAGGAGCATGACAATCCGTTCACGCTCTATCCTTATGACACCAACTACCTCATTTACACCCAAACCAGCGATCTGAATAAAGAAGCGATTGCCAGTTACGACTGGGCGGAAAATGCGCGTAAGGATGAAGTAAAGTTTCAGTTGAGCCTGGCATTTCCGCTGTGGCGTGGGATTTTAGGCCCGAACTCGGTGTTGGGTGCGTCTTATACGCAAAAATCCTGGTGGc        <  2:657283/288‑1 (MQ=255)
                                                                                                                                                                                                                                                                      aaCGGTGAAAGAGGTGCATGACGCGCCAGCGGTGCGTGGCAGtattat                                                                                                                                                                                                                                                       <  1:320860/48‑1 (MQ=255)
                                                                                                                                                                                                                                                                      aaCGGTGAAAGAGGTGCATGACGCGCCAGCGGTGCGTGGCAGtattat                                                                                                                                                                                                                                                       >  2:320860/1‑48 (MQ=255)
                                                                                                                                                                                                                                                                      aaCGGTGAAAGAGGTGCATGACGCGCCAGCGGTGCGTGGCAGTATTATCGCCAATATGCTGCAGGAGCATGACAATCCGTTCACGCTCTATCCTTATGACACCAACTACCTCATTTACACCCAAACCAGCGATCTGAATAAAGAAGCGATTGCCAGTTACGACTGGGCGGAAAATGCGCGTAAGGATGAAGTAAAGTTTCAGTTGAGCCTGGCATTTCCGCTGTGGCGTGGGATTTTAGGCCCGAACTCGGTGTTGGGTGCGTCTTATACGCAAAAATCCTGGTGGCaa      <  1:646696/289‑1 (MQ=255)
                                                                                                                                                                                                                                                                      aaCGGGGAAATAGGTGCATGACGGGCCAGCGGTGCGTGGCAGTATTATCGCCAATATGCTGCAGGAGCATGACAATCCGTTCACGCTCTATCCTTATGACACCAACTACCTCATTTACACCCAAACCAGCGATCTGAATAAAGAAGCGATTGCCAGTTACGACTGGGCGGAAAATGCGCGTAAGGATGAAGTAAAGTTTCAGTTGAGCCTGGCATTTCCGCTGTGGCGTGGGATTTTAGGCCCGAACTCGGTGTTGGGTGCGTCTTATACGCAAAAATCCTGGTGGCaa      <  2:393765/289‑1 (MQ=255)
                                                                                                                                                                                                                                                                           tGAAAGAGGTGCATGACGCGCCAGCGGTGCGTGGCAGTATTATCGCCAATATGCTGCAGGAGCATGACAATCCGTTCACGCTCTATCCTTATGACACCAACTACCTCATTTACACCCAAACCAGCGATCTGAATAAAGAAGCGATTGCCAGTTACGACTGGGCGGAAAATGCGCGTAAGGATGAAGTAAAGTTTCAGTTGAGCCTGGCATTTCCGCTGTGGCGTGGGATTTTAGGCCCGAACTCGGTGTTGGGTGCGTCTTATACGCAAAAATCCTGGTGGCAACTGt  >  2:433221/1‑288 (MQ=255)
                                                                                                                                                                                                                                                                           tGAAAGAGGTGCATGACGCGCCAGCGGTGCGTGGCAGTATTATCGCCAATATGCTGCAGGAGCATGACAATCCGTTCACGCTCTATCCTTATGACACCAACTACCTCATTTACACCCAAACCAGCGATCTGAATAAAGAAGCGATTGCCAGTTACGACTGGGCGGAAAATGCGCGTAAGGATGAAGTAAAGTTTCAGTTGAGCCTGGCATTTCCGCTGTGGCGTGGGATTTTAGGCCCGAACTCGGTGTTGGGTGCGTCTTATACGCAAAAATCCTGGTGGCAACTGt  >  1:619043/1‑288 (MQ=255)
                                                                                                                                                                                                                                                                                    tGCATGACGCGCCAGCGGTGCGTGGCAGTATTATCGCCAATATGCTGCAGGAGCATGACAATCCGTTCACGCTCTATCCTTATGACACCAACTACCTCATTTACACCCAAACCAGCGATCTGAATAAAGAAGCGATTGCCAGTTACGACTGGGCGGAAAATGCGCGTAAGGATGAAGTAAAGTTTCAGTTGAGCCTGGCATTTCCGCTGTGGCGt                                                                  >  2:178565/1‑215 (MQ=255)
                                                                                                                                                                                                                                                                                    tGCATGACGCGCCAGCGGTGCGTGGCAGTATTATCGCCAATATGCTGCAGGAGCATGACAATCCGTTCACGCTCTATCCTTATGACACCAACTACCTCATTTACACCCAAACCAGCGATCTGAATAAAGAAGCGATTGCCAGTTACGACTGGGCGGAAAATGCGCGTAAGGATGAAGTAAAGTTTCAGTTGAGCCTGGCATTTCCGCTGTGGCGt                                                                  <  1:178565/215‑1 (MQ=255)
                                                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                                                     
GGCTTGTTCAGCGGTCAGTACGGCAGACATATCATCCTTACACTTCATTGGTTAATGAAATGTTGATTTTATGCTTCTTTGTTGTTGGTTTCTACTTTAGGAAGGGATAACTAACGGCTATGGAGTTAAGTATGTAAAATAGCCCGCAGAAAAATATTCACCTTATCAATAATTCGTTACGGAGAACACGACCGATGCGGACTCTGCAGGGCTGGTTGTTGCCGGTGTTTATGTTGCCTATGGCAGTATATGCACAAGAGGCAACGGTGAAAGAGGTGCATGACGCGCCAGCGGTGCGTGGCAGTATTATCGCCAATATGCTGCAGGAGCATGACAATCCGTTCACGCTCTATCCTTATGACACCAACTACCTCATTTACACCCAAACCAGCGATCTGAATAAAGAAGCGATTGCCAGTTACGACTGGGCGGAAAATGCGCGTAAGGATGAAGTAAAGTTTCAGTTGAGCCTGGCATTTCCGCTGTGGCGTGGGATTTTAGGCCCGAACTCGGTGTTGGGTGCGTCTTATACGCAAAAATCCTGGTGGCAACTGT  >  NC_000913/4004668‑4005222

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑