| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 3,206,484 | C→A | 22.3% | Q8K (CAA→AAA) | ttdA → | L‑tartrate dehydratase, alpha subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,206,484 | 0 | C | A | 22.3% | 53.4 / 3.1 | 35 | Q8K (CAA→AAA) | ttdA | L‑tartrate dehydratase, alpha subunit |
| Reads supporting (aligned to +/- strand): ref base C (10/17); new base A (4/4); total (14/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.85e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.79e-01 | |||||||||||
ATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAG‑ACAAATACAAAAACTGGAGTTGCCATGATGA‑GCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTA‑CCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTC > NC_000913/3206248‑3206716 | aTTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAG‑ACAAATACAAAAACTGGAGTTGCCATGATGA‑GCGAAAGTAATAAGCaa < 1:285995/239‑1 (MQ=255) ttCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGGGGAATTTTTCAC‑AAAAAAACAAAAAATGGAGTTGTCCTTGTGT‑GGGAAAGTAATAAGAAAc > 2:250348/1‑239 (MQ=255) tCAGCATCGTTAATTATCCGCAGTTTTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCTTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAG‑ACAAATACAAAAACTGGAGTTGCCATGATGA‑GCGAAAGTAATAAGCAACa < 2:341723/239‑1 (MQ=255) cATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTTAT‑ACCAAAAAAAAAAATCGAGTTGGCCTGATTA‑GCGACAGGAATAAACaaacagca > 2:25988/1‑233 (MQ=255) aTCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAG‑ACAAATACAAAAACTGGAGTTGCCATGATGA‑GCGAAAGTAATAAGCAACAGGc < 1:195128/237‑1 (MQ=255) ttAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGAGAATTTTTTCAG‑ACAAAAAAAAAAAATTGAGTTTCCATGGTGA‑GCGAAAGGAATAAGAAAAAGGCAATGaa > 1:103531/1‑239 (MQ=255) aGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAAATTTTTTAA‑ACAAAAACAAAAAAAGTAGTTGCCATGATGA‑GCGGAAGTAATAAACAACAAGCAGTGAATAAGATTACAGAGATTTTAGCt > 1:13288/1‑239 (MQ=255) aaaaCATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCTGAAAAAATAC‑AAAACAGGAGATGTCATGTAGAGGGGAAATAAAAAAAAAACAGGAAGAGAAAAGATTAACA‑AGATTGTTGCTACATTTAACACCATggt > 1:389349/1‑237 (MQ=255) aaCATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCCA‑CCAAAAAAAAAAACTGTAGATGGCGTTTAGA‑GGGAAAGTAATAAACAACAAGAACTGAATAAATTGATAGAGAGTGTTAGCAACTTTACCGCCCTGAatt > 2:54487/1‑239 (MQ=255) ctttGCCTTTCCCTGCTCTGGCGAAATATTCATTCTTATTTTCGTGTTAATTCACAAACTGTTAATTAAATATTCAGGGTGGCAAAAAATAAAAAACCACATTTTTAGTGGTAGTTTGGGGGGGTGAATTTTTCCAA‑AAAAATACAAAAACTGGAGTGGCCATGATGA‑GCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCt < 2:13288/237‑1 (MQ=255) cATTCCCCGCTCGGGCGAAATATTCATTCTTATTTTCGTGTTGATTCACGAACTGTTAATTAAATATTCACGGTGGAAAAAAATATAAAACCACATTTTTAGGGGTAGTTGGTGGGGGTGAATTTTTCAAG‑ACAAATAAAAAAACTGGGTTTGCCATGATGA‑GCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTGGTTAACTTTACCGCCATGATTTCTA‑CCCGa < 2:389349/239‑1 (MQ=255) aTTCCCTGCTCTGGCTAAATTTTAATTCTTTTTTCCGCGTTATTTCACGAACTGTTAATTACATATTCGCGGTGGCAAAAAATATAAAACCACATTTTTATGGGTAGTTTGTGGGGGTAAATTTTTCCAG‑ACAAATACAAAAACTGGAGTTGCCATGATGA‑GCGAAAGTAATAAGCAACAGGCAGTAAATAAGTTGACAGAGATTGTCGTTAACTTTACCGCCATGATTTCTA‑CCCGaa < 2:210357/239‑1 (MQ=255) tGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGGAAATTTTCAG‑ACAAATAAAAAAAATTGAATTGCCATGGTGA‑GGGGAAGTAATAAGCAAAACGCAGTGAATAAATTTGCAGAGATTGTTGCTAACATTACCGGCCTGATTTTTATCCCGatagctg > 1:435241/1‑233 (MQ=255) ggCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGAGAGATTTTTTAG‑ACAAAAACAAAAACAAGAGTTGCGATGGTGA‑GCGCAAGTAATAAACAAAAGAGAGGGAATAAATTGACAGAGAATTTTGCTCACTTTACCGCCACGATTTTTT‑ACCGCAAGCCtgtttatg > 1:150395/1‑233 (MQ=255) gcgcAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGGATTTTTTTAG‑AAAAAAAAAAAAAATGGAGTTTGCATGAGTA‑GGGGAAGGTATAAACAAAAAGCAAGGAAAAAAGTTACAGAGATTGTCGCTAAATTTACACCCCTGATTTTCA‑ACCGAAAGCCtggttgaat > 2:57195/1‑232 (MQ=255) gcAATATTCATTTTTATTTTCGCTTTGATTAACAAACTGTTAATTATATATTCTGGGTGGCAAAAAATATAAAACCACATTTTGAGTGGTAGTTTGGGGCGGTGAATTTTTCAAG‑AAAAATAAAAAAACTGGAGTTGCCATGATGA‑GCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTA‑CCCGAATGCCTGATGACgtg < 2:435241/238‑1 (MQ=255) tatTCATTCTTATTTTGGCGTTAATTCACAAACTGTTAATTACATATTGAGGGTGGAAAAAAATATAAACCCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTACCAG‑AAAAATACAAAAACTGGAGTTGCCAGGATGA‑GCGAAATTAATAAGCAACAGGCAGAGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTA‑CCCGAATGCCTGATGACGTGGTGGa < 1:25988/239‑1 (MQ=255) tatTCATTCTTATTTACGCGTTGATACACGAACTTTTAATTACATTTTCCCGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAG‑ACAAATACAAAAACTGGAGTTGCCATGATGA‑GCGAAAGTAATAAGCAACAGGCAGTAAATAAGTTGACAGAGATTGTCGCTACCTTTCCCGCCATGATTTCTA‑CCCGAATGCCTGATGACGTGGTGGa < 2:266158/239‑1 (MQ=255) tttCGCTTTAATTCGAGAACTTTTAATTACATATTCACGGTGGCAAAAAATAAAAACCCACATTTTTAGTGGTATTTTGTGGCGGTGAATTTTTCAAA‑AAAAATAAAAAAACTGGATTTGCCATGATGA‑GCAAAAGTAATAACCAACGGGCAGTAAATAATTTGACAGAAATTGTCGCTAACTTTACCGCCATGATTTCTA‑CCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAg < 1:12985/239‑1 (MQ=255) tgaaTTACATATTCACGGTGGAAAAAATAATAAACACCCATTTTAATTGGTATTTTGGTGGGGTTAATTTTCCAAA‑ACAAATAAAAAAACTGGAGTTGCCATGAGGA‑GCTAATGTATTAAGAAACAGGCAGTAAAAAATTTGACAGAGATTGTCGTGAACTTTACCGCCATGATTTCTA‑CCCGAATGCCTGATGACGTGGTGGATAAACTaaaa < 1:363901/212‑1 (MQ=255) tatTCAGGTGGGAAAAAAATAAAAAACCACATTTTTAGTGGTAGTTTGGGGCGGTGAATTTTTCCAG‑AAAAATACAAAAACTGGAGTTGCCATGATGA‑GCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTAACCGCCATGATTTCTA‑CCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGa < 2:103531/239‑1 (MQ=255) tatTCACGGTGGCAAAAAAAAAAAACCCACATTTTGAGTGGTAGTTTGTGGCGGTGAATTTTCCCAA‑CAAAAAACAAAAACTGGGGTTGCCATGATAA‑GCAAAATTAATAAGCAACAGACAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTA‑CCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGa < 1:287084/239‑1 (MQ=255) atTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAA‑AGAAAAACAAAAAAACGAGGTTTCATGTTGA‑GCGAAAGTAAAAAACAACACGAAGTGAATAAGATGTCAGAGACTTTCGCTAACATTATCACCAAGATTTCTA‑TCCCAAAGTCTGATAACGTTGTGGTGAAACTAAAACAGATaaaag > 1:99391/1‑212 (MQ=255) ggagaaaaaaaTAAAAAACCATATTTTTAGGGGTAGTTTGGGGCGGTAAATTTTTCCAA‑AAAAATAAAAAAACTGGAGTTGACATGATGA‑GCAAAAGAAATAAGCAACAGGAAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTA‑CCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTAt < 2:219371/233‑1 (MQ=255) tGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAAATTTTTCAG‑ACAAAAACAAAAAAATGAGGTGGCATGATGA‑GGGGAAGGAATAAAAAAAAAGCAAAGAAAAAATTGAAAGAGAATGTTGCTAAATTTTACGCCATGATTTTTA‑CCCCAAAGCCTGATGACGTGGGGGATAAACTAAAAAAGCTAAAAGATGCCGGAACGACGTCGATTGGGGAAATTATCt > 1:170046/1‑239 (MQ=255) aaTAAAAACCAACATTTTTAGTGGTATTTTGGGGGGGTAAATTTTCCCAA‑AAAAATACAAAAACGGGAGTTGCCATGAGGA‑GCGAAAGAAAAAAACAACAGCCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTAGCCGCCATGATTTCTA‑CCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACg < 1:92411/239‑1 (MQ=255) taaaaaaCCAAATTTTTAGTGGTAGTTTGGGGGGGTGAATTTTTCAAG‑AAAAATAAAAAAACGGGAGTTGCCAGGATGA‑GCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTA‑CCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGa < 2:187751/217‑1 (MQ=255) gggTAGTTGGGGGCGGTGAATTTTTCAAG‑AAAAAAAGAAAAACTGGAGTGGCCATAATGA‑GAAAAAGTAATAAGAAACGGGCAGTGAATAAGTTGAAAGGGATTGTGACTAATTGTACCGCCATGATTTCTA‑CCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCaaaa < 1:250348/237‑1 (MQ=255) aaaaaaaaaaaaaTTGAATTTGCAAGAATAA‑GCGAAAGTAACAAGAAACGGGAGGAAAATAAGTTGCAAGAAATGGCCGCTAACTTTACCGCCATGATTTCTA‑CCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCa < 1:57195/231‑1 (MQ=255) cAAATACAAAAACTGGAGTTGCCATGATGA‑GCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTA‑CCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAg > 1:507347/1‑239 (MQ=255) ataagagaaatttGCCATGATGA‑GCGAAAGTAATAAGCAACAGGCAGTAAAAAAGTTGACAGAGTTTGTCGTAAACTTTACCGCCATGATTTCTA‑CCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCg < 2:411135/228‑1 (MQ=255) aaaCTGGAGTTGCCATGATGA‑GCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTA‑CCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCggg > 2:307976/1‑239 (MQ=255) aaCTGGGGTTGCAAGGAGGA‑GGAAAAGTAAAAAGCAAGGGGAGGGGAATAGATTGAAGGAGATTGCGGCAAACTTAACCGCCATGATTTCTA‑CCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCgggg < 2:150395/239‑1 (MQ=255) a‑GCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTA‑CCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGAGAGATTAt > 1:411135/1‑227 (MQ=255) aataaCAAACAGGAAGTGAATAGTTTGACAGAGTTGTTCGTAAACTTTACCGCCATGATTTCTA‑CCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTc < 1:304520/239‑1 (MQ=255) | ATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAG‑ACAAATACAAAAACTGGAGTTGCCATGATGA‑GCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTA‑CCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTC > NC_000913/3206248‑3206716 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |