BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	7,733	T→G	16.7%	N76T (AAC→ACC)	yaaJ ←	putative transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	7,733	0	T	G	16.7%	245.1 / 5.4	107	N76T (AAC→ACC)	yaaJ	putative transporter
Reads supporting (aligned to +/- strand): ref base T (33/51); new base G (19/2); total (54/53)
Fisher's exact test for biased strand distribution p-value = 1.92e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 108 total aligned reads displayed.

GCCATAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGACGTCACGTTCTTTATAAAGTTGTGCCAGAGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACAT‑CCAAAAGACGGCTCCAGGTCCACCGGCGGTAATAGCCAGCGCAACGCC‑GGCCAGGTTGCCGCTACCCACGCGCGCCG‑CAAGACTGGTACACAATGAC‑TGAAATGAGGTTAAACCGCCTGGCTGTGGATGAATGCTA‑TTTTTAAG‑ACTTTTGCCAAACTGGCGGATGT‑AGC‑GAAAC‑TGCACAAATCC‑GGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAGGACGCTGTTAATGAAGGAGAAAAAATCTGGCATGCATATCC  >  NC_000913/7495‑7967

gCCATAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGACGTCACGTTCTTTATAAAGTTGTGCCAGAGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACAT‑CCAAAAGACGGCTCCAGGTCCACCGGCGGTAATAGCCAGCGCAACGCC‑GGCCAGGt                                                                                                                                                                                                                                                    <  2:1187201/239‑1 (MQ=255)

         gATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGACGTCACGTTCTTTATAAAGTTGTGCCAGAGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACAT‑CCAAAAGACGGCTCCAGGTCCACCGGCGGGAATAGCCCGCGCAAACCC‑CAGCAGGGTGCCGcgc                                                                                                                                                                                                                                            >  2:885757/1‑236 (MQ=255)

           tGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGACGTCACGTTCTTTATAAAGTTGTGCCAGAGCACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACAT‑CCAACAGCCGGCTCCAGGTCCACCGGCGGTAATAACCAGCGCAAAGCC‑GGCCCGGGTGCCGCTAcc                                                                                                                                                                                                                                          >  1:860106/1‑238 (MQ=255)

                aaaacGCCGGCGAACAGAACGCCCATCAAGCGCATCCCCCGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGACGTCACGTTCTTTATAAAGTTGTGCCAGAGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACAT‑CCAAAAGACGGCTCCAGGTCCACCGGCGGTAATAGCCAGCGCAACGCC‑GGCCAGGTTGCCGCTACCCAcgcg                                                                                                                                                                                                                                    <  2:348325/239‑1 (MQ=255)

                aaaaaGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGACGTCACGTTCTTTATAAAGTTGTGCCAGAGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACAT‑CCAAAAGACGGCTCCAGGTCCACCGGCGGTAATAGCCAGCGCAACGCC‑GGCCAGGTTGCCGCTACCCAcgcg                                                                                                                                                                                                                                    <  1:1143506/239‑1 (MQ=255)

                aaaaaGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCAGTGACGTCACGTTCTTTATAAAGTTGTGCCAGAGACCATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACAT‑CCAAAAGACGGCTCCAGGTCCACCGGCGGTAATAGCCAGCGCAACGCC‑GGCCAGGTTGCCGCTACCCAcg                                                                                                                                                                                                                                      <  1:754457/237‑1 (MQ=255)

                         gCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGACGTCACGTTCTTTATAAAGTTGTGCCAGAGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACAT‑CCAAAAGACGGCTCCAGGTCCACCGGCGGTAATAGCCAGCGCAACGCC‑GGCCAGGGTGCCGCTACCCACGCGCGCCG‑CAAg                                                                                                                                                                                                                          >  1:227152/1‑239 (MQ=255)

                            aaCAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGACGTCACGTTCTTTATAAAGTTGTGCCAGAGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACAT‑CCAAAAGACGGCTCCAGGTCCACCGGCGGTAATAGCCAGCGCAACGCC‑GGCCAGGTTGCCGCTACCCCCGCGCGCCG‑CAAg                                                                                                                                                                                                                          >  1:907311/1‑236 (MQ=255)

                              cAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGACGTCACGTTCTTTATAAAGTTGTGCCAGAGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACAT‑CCAAAAGTCGGCTCCAGGTCCACCCGCGGTAATAGCCAGCGCCAGCCC‑CGCCCTGTCGCCGCTCCCCACGCGAGCCG‑Cacagctcg                                                                                                                                                                                                                     >  2:818768/1‑232 (MQ=255)

                                gAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGACGTCACGTTCTTTATAAAGTTGTGCCAGAGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACAT‑CCAAAAGACGGCTCCAGGTCCACCGGCGGTAATAACCACCGCCACACC‑GGCCAGGTTGCCGCCAACCACCCGCGCCC‑CAAGACCggga                                                                                                                                                                                                                   >  1:1337632/1‑237 (MQ=255)

                                      ccATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGACGTCACGTTCTTTATAAAGTTGTGCCAGAGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACAT‑CCAAAAGACGGCTCCAGGTCCACCGGCGGTAATAACCAGCGCAAACCC‑CGCCCGGGTGCCGCTACCCCCGCGCGCCG‑CCAGACTGGGCCACAAt                                                                                                                                                                                                             >  1:114147/1‑239 (MQ=255)

                                         cccacggatcccccAGCCGGCGCGCCATATACCATGCCGGTCCGCCACGAACCTGCCCATTGACGTCACGTTCTTTATAAAGTTGTGCCAGAGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACAT‑CCAAAAGACGGCTCCAGGTCCACCGGCGGTAATAGCCAGCGCAACGCC‑GGCCAGGTTGCCGCTACCCACGCGCGCCG‑CAAGACTGGTACACAATGAc                                                                                                                                                                                                          <  2:415760/229‑1 (MQ=255)

                                              cgcccccccAGCCCCCGCCCCAGATCCCATCCCGTTCCGCCACGAAACTGCCCATGGGCGTCACGTTCTTTATAAAGTTGTGCCAAAGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACAT‑CCAAAAGACGGCTCCAGGTCCACCGGCGGTAATAGCCAGCGCAACGCC‑GGCCAGGTTGCCGCTACCCACGCGCGCCG‑CAAGACTGGTACACAATGAC‑TGaaa                                                                                                                                                                                                    <  2:189185/234‑1 (MQ=255)

                                              cgcATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGACGTCACGTTCTTTATAAAGTTGTGCCAGAGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACAT‑CCAAAAGACGGCTCCAGGTCCACCGGCGGTAATAACCAGCCCAACACC‑GGCCCGGTTGGCGCTACCCCCGCGCGCCG‑CCAAGCTGGGACACAAAGAC‑TGaaa                                                                                                                                                                                                    >  2:75802/1‑239 (MQ=255)

                                               gcATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGACGTCACGTTCTTTATAAAGTTGTGCCAGAGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACAT‑CCAAAAGACGGCTCCAGGTCCACCGGCGGTAATAGCCAGCGCAACGCC‑GGCCAGGTTGCCGCTACCCACGCGCGCCG‑CAAGACTGGTACACAATGAC‑TGAAAt                                                                                                                                                                                                   <  1:1415305/239‑1 (MQ=255)

                                                cATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGACGTCACGTTCTTTATAAAGTTGTGCCAGAGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACAT‑CCAAAAGACGGCTCCAGGTCCACCGGCGGAAATAACCAGACCAACCCC‑GGCCCGGTTGCCGCTACCCACGCGCGCCG‑CAAGACCGGGACACAATGAC‑TGAAATg                                                                                                                                                                                                  >  1:1087405/1‑239 (MQ=255)

                                                   ccccAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGACGTCACGTTCTTTATAAAGTTGTGCCAGAGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACAT‑CCAAAAGACGGCTCCAGGTCCACCGGCGGTAATAGCCAGCGCAACGCC‑GGCCAGGTTGCCGCTACCCACGCGCGCCG‑Caa                                                                                                                                                                                                                           <  1:63210/212‑1 (MQ=255)

                                                             cgcgcCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGACGTCACGTTCTTTATAAAGTTGTGCCAGAGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACAT‑CCAAAAGACGGCTCCAGGTCCACCGGCGGTAATAGCCAGCGCAACGCC‑GGCCAGGTTGCCGCTACCCACGCGCGCCG‑CAAGa                                                                                                                                                                                                                         >  1:1021502/1‑204 (MQ=255)

                                                             cgcgcCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGACGTCACGTTCTTTATAAAGTTGTGCCAGAGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACAT‑CCAAAAGACGGCTCCAGGTCCACCGGCGGTAATAGCCAGCGCAACGCC‑GGCCAGGTTGCCGCTACCCACGCGCGCCG‑CAAGa                                                                                                                                                                                                                         <  2:1021502/204‑1 (MQ=255)

                                                             cgcgcCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGACGTCACGTTCTTTATAAAGTTGTGCCAGAGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACAT‑CCAAAAGACGGCTCCAGGTCCACCGGCGGTAATAACCAGCGCAACGCCGGGCCAGG‑TGCCGCTACCCCCGCCCGCCC‑CACGACTGGGACACACAGAC‑CGGAATGAGGGTAAACCGc                                                                                                                                                                                      >  2:1288884/1‑238 (MQ=255)

                                                             cgcgcCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGACGTCACGTTCTTTATAAAGTTGAGCCAGAGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACAT‑CCAAAAGACGGCTCCAGGTCCACCGGCGGTAATAACCCGCGCAACACC‑CGCCCGGGTGCCCCCAACCCCGCCCGCCGCCAAGA‑TGGGACACAATGAC‑TAGTACGAGGTTAAAcccc                                                                                                                                                                                      >  2:743324/1‑236 (MQ=255)

                                                                gcCAAATCCCATGCCGGGCCGCCACGAAACTGCCCATTGACGTCACGTTCTTTATAAAGTTGTGCAAGAGAACATCCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACAT‑CCAAAAGACGGCTCCAGGTCCACCGGCGGTAATAGCCAGCGCAACGCC‑GGCCAGGTTGCCGCTACCCACGCGCGCCG‑CAAGACTGGTACACAATGAC‑TGAAATGAGGTTAAACCGCCTgg                                                                                                                                                                                  <  2:907311/239‑1 (MQ=255)

                                                                   atatCCCATGCCGGTCCGCCACGAAACTGCCCATTGACGTCACGTTCTTTATAAAGTTGTCCCAGAGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACAT‑CCAAAAGACGGCTCCAGGTCCACCGGCGGTAATAGCCAGCGCAACGCC‑GGCCAGGTTGCCGCTACCCACGCGCGCCG‑CAAGACTGGTACACAATGAC‑TGAAATGAGGTTAAACCGCCTGGCtg                                                                                                                                                                               <  1:75802/239‑1 (MQ=255)

                                                                       cccATGCCGGTCCGCCCAGAAACGACCCTTTGACGTCCCGTTTTTTAAAAAGTTGGCCCAGAAAACATTCGCAAGACGAGGTCGCCATGCCGATAAACGCGGCACCCCACAT‑CCAAAAGACGGCTCCAGGTCCACCGGCGGTAATAGCCAGCGCAACGCC‑GGCCAGGTTGCCGCTACCCACGCGCGCCG‑CAAGACTGGTACACAATGAC‑TG‑AATGAGGTTAAACCGCCTGGCTGTGGAt                                                                                                                                                                          <  2:183550/238‑1 (MQ=255)

                                                                        ccATGCCGGTCCGCCACGAAACTGCCCATTGCCGTCACTTTCTTTATAAAGTGGTGCCAGAGAACTTCCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACAT‑CCAAAAGACGGCTCCAGGTCCACCGGCGGTAATAGCCAGCGCAACGCC‑GGCCAGGTTGCCGCTACCCACGCGCGCCG‑CAAGACTGGTACACAATGAC‑TGAAATGAGGTTAAACCGCCTGGCTGTGGAt                                                                                                                                                                          <  1:1108755/239‑1 (MQ=255)

                                                                           tGCCGGTCCGCCACGAAACTGCCCATTGACGTCACGTTCTTTATAAAGTTGTGCCAGAGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACAT‑CCAAAAGACGGCTCCAGGTCCCCCGGCGGTAATAGCCAGCGCAACGCC‑GGCCAGGTTGCCGCTACCCACGCGCGCCG‑CAAGACTGGTACACAATGAC‑TGAAATGAGGTTAAACCGCCTGGCTGTGGATGaa                                                                                                                                                                       <  1:817117/239‑1 (MQ=255)

                                                                            gCCGGTCCGCCACGAAACTGCCCATTGACGTCACGTTCTTTATAAAGTTGTGCCAGAGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACAT‑CCAAAAGACGGCTCCAGGTCCACCGGCGGTAATAGCCAGCGCAACGCC‑GGCCCGGTTGCCGCCACCCCCGCGCGCCG‑CAAGAATGGTACACACTGAC‑TGAAAAGAGGTTAAACCGCCCGGCTGTGGATGAAt                                                                                                                                                                      >  1:1081197/1‑239 (MQ=255)

                                                                            gCCGGTCCGCCACGAAACTGCCCATTGACGTCACGTTCTTTATAAAGTTGTGCCAGAGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACAT‑CCAAAAGACGGCTCCAGGTCCACCGGCGGTAATAGCCAGCGCAACGCC‑CGCCAGGGTGCCGCTACCCACCCGCCCCG‑CCAAACTGGGAAACAATGAC‑TGAAATGAGGTTAAACCGACTGGGtggtgtggaat                                                                                                                                                                      >  2:392518/1‑230 (MQ=255)

                                                                            gCCGGTCCGCCACGAAACTGCCCATTGACGTCACGTTCTTTATAAAGTTGTGCCAGAGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACAT‑CCAAAAGACGGCTCCAGGTCCACCGGCGGTAATAACCCGCGCAACGCC‑GGCCAGGGTGCCCCTACCCCCGCGCGCCC‑CCCGACCGGGACACAATGAC‑TGAAAAGAGGTTTAACCGCCCGGGTGTGGGTGaaa                                                                                                                                                                      >  2:1409775/1‑238 (MQ=255)

                                                                            gCCGGTCCGCCACGAAACTGCCCATTGACGTCACGTTCTTTATAAAGTTGTGCCAGAGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACAT‑CCAAAAGACGGCTCCAGGTCCACCGGCGGTAATAACCAGCCCCCCGCC‑CGCCAGGGTGCCGCTCCCCACCCCCGCCG‑CAAGACTGGTACACAATGAC‑TGCAATGAGGTTAAACCGCCCCGCAGTGGATg                                                                                                                                                                         >  2:716656/1‑236 (MQ=255)

                                                                            gCCGGTCCGCCACGAAACTGCCCATTGACGTCACGTTCTTTATAAAGTTGTGCCAGAGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACAT‑CCAAAAGACGGCTCCAGGTCCACCGGCGGTAATAACCAGCCCAACCCC‑CGCCAGGGTTCCGCTACCCACGCGCGCCG‑CCAGACTGGTACACAATGAC‑CGAAATGAGGGTAAACCGCCTTGCTGGGGGTGAAt                                                                                                                                                                      >  1:1108594/1‑239 (MQ=255)

                                                                            gCCGGTCCGCCACGAAACTGCCCATTGACGTCACGTTCTTTATAAAGTTGTGCCAGAGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACAT‑CCAAAAGACGGCTCCAGGTCCACCGGCGGTAACAACCACCGCCACGCC‑GGCCACGTTGCCGCCTCCCACGCCCCCCA‑CCACACCGGGTCACAATACC‑CGCAATGAGGTTAAACCCCCTTGccttggtggaat                                                                                                                                                                      >  2:1283720/1‑228 (MQ=255)

                                                                               ggtccccacagAACCTGCCCATTGACGCCACGTTCTTTATAAAGTTTGGCCAGAGAACATTCGGCAAAGGAGGGCGCCATGCCGATAACCGCGGCAACCCACAT‑CCAAAAGGCGGCTCCAGGTCCACCGGCGGTAATAGCCTGCGCAACCCC‑GGCCAGGTTGCCGCTACCCACGCGCGCCG‑CAAGACTGGTACACAATGAC‑TGAAATGAGGTTAAACCGCCTGGCTGTGGATGAATGCt                                                                                                                                                                   <  2:706575/229‑1 (MQ=255)

                                                                                gTCCGCCACGAAACTGCCCATTGACGTCACGTTCTTTATAAAGTTGTGCCAGAGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACAT‑CCAAAAGACGGCTCCAGGTCCACCGGCGGTAATAGCCAGCGCAACGCC‑GGCCCGGTTGCCGCTACCCACGCGCGCCG‑CAAGACTGGTACACAATGAC‑TGAAATGAGGTTAAACCGCCTGGCTGTGGGTGAATGCTa                                                                                                                                                                  >  1:581792/1‑239 (MQ=255)

                                                                                gTCCGCCACGAAACTGCCCATTGACGTCACGTTCTTTATAAAGTTGTGCCAGAGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACAT‑CCAAAAGACGGCTCCAGGTCCACCGGCGGTAATAGCCAGCGCAACGCC‑GGCCAGGTTGCCGCTACCCACGCGCGCCG‑Caa                                                                                                                                                                                                                           <  1:1049826/183‑1 (MQ=255)

                                                                                gTCCGCCACGAAACTGCCCATTGACGTCACGTTCTTTATAAAGTTGTGCCAGAGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACAT‑CCAAAAGACGGCTCCAGGTCCACCGGCGGTAATAGCCAGCGCAACGCC‑GGCCAGGTTGCCGCTACCCACGCGCGCCG‑CAAGACTGGTACACAATGAC‑TGaaa                                                                                                                                                                                                    <  2:133447/205‑1 (MQ=255)

                                                                                gTCCGCCACGAAACTGCCCATTGACGTCACGTTCTTTATAAAGTTGTGCCAGAGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACAT‑CCAAAAGACGGCTCCAGGTCCACCGGCGGTAATAGCCAGCGCAACGCC‑GGCCAGGTTGCCGCTACCCACGCGCGCCG‑CAAGACTGGTACACAATGAC‑TGaaa                                                                                                                                                                                                    >  1:133447/1‑205 (MQ=255)

                                                                                 tCCGCCACGAAACTGCCCATTGACGTCACGTTCTTTATAAAGTTGTGCCAGAGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACAT‑CCAAAAGACGGCTCCAGGTCCACCGGCGGTAATAGCCAGCGCAACGCC‑GGCCCGGTTGGCGCTACCCACGCGCGCCG‑CAAGACAGGGAAAAAATGAC‑TGAAATGAGGTTAAACCGCCCGGCTGGGGGAGAAAGCTA‑t                                                                                                                                                                >  2:555033/1‑239 (MQ=255)

                                                                                     ccACGAAACTGCCCATTGACGTCACGTTCTTTATAAAGTTGTGCCAGAGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCCAAAAGACGGCTCCAGGTCCACCGGCGGGAAAAGGCAGCGGAACACC‑GGCCAGGTTGGCGCCACCCACCACCGCCG‑CAAGACTGGTAAACAATGCA‑TGAAATGAGGTTAAAACGCCTGGCTGTGGATGGATGCAA‑tttt                                                                                                                                                             >  2:511917/1‑239 (MQ=255)

                                                                                          aaaCTGCCCATTGACGTCACGTTCTTTATAAAGTTGTGCCAGAGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACAT‑CCAAAAGACGGCTCCAGGTCCACCGGCGGTAATAGCCAGCGCAACGCC‑GGCCAGGTTGCCGCTACCCCCCCGCGCCG‑CCAGACTGGGaca                                                                                                                                                                                                                 >  2:1049826/1‑183 (MQ=255)

                                                                                                cccATTGACGTCACGTTCTTTATAAAGTTGTGCCAGAGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACAT‑CCAAAAGACGGCTCCAGGTCCACCGGCGGTAATAGCCAGCGCAACGCC‑GGCCAGGTTGCCGCTAACCACGCGCGGCG‑CAAGACTGGTAACCAAAGAC‑TGAAATGAGGTTAAAACGCCTGGCTGGGGGTGAATGCTA‑TTTTTTAG‑AAttttt                                                                                                                                                 >  2:284720/1‑237 (MQ=255)

                                                                                                  catgggcGTCAGGTTCTTTAAAAAGTTGTGCCGAAAAACATGCGGCAAACGCGGTCGCCATGCCGATAAACGCGGCAACCCACAG‑CCAAAAGACGGCTCCAGGTCCACCGGCGGTAATAGCCAGCGCAACGCC‑GGCCAGGTTGCCGCTACCCACGCGCGCCG‑CAAGACTGGTACACAATGAC‑TGAAATGAGGTTAAACCGCCTGGCTGTGGATGAATGCTA‑TTTTTAAG‑ACTTTTGCCa                                                                                                                                              <  2:860106/233‑1 (MQ=255)

                                                                                                          tCACGTTCTTTATAAAGTTGTGCCAGAGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACAT‑CCAAAAGACGGCTCCAGGTCCACCGGCGGTAATAGCCAGCGCAACGCC‑GGGCAGGTTGCCGCTACCCACGCGCGCCG‑CAAGAATGGTACACAAAGGC‑TGAAATGAGGGTAAAACGCCTGGCTGGGGGTGGATGCTA‑TTTTTAAG‑ACTTTTGCCAAACTGGCg                                                                                                                                      >  2:229462/1‑239 (MQ=255)

                                                                                                          tCACGTTCTTTATAAAGTTGTGCCAGAGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACAT‑CCAAAAGACGGCTCCAGGTCCACCGGCGGCAATAGCCAGCGCAACGCC‑GGCCCGGGTGCCGCCACCCACGCGCGCCG‑CAAGACTGGGACACAATGACTTGAATAGAGG‑TAAACCCCGCGGCTGGGGGTGGATGCCA‑TTTTTAAC‑AGTTTTGcctcaccgggg                                                                                                                                      >  2:1237027/1‑230 (MQ=255)

                                                                                                                    tataAAGTTGTGCCAGAGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACAT‑CCAAAAGACGGCTCCAGGTCCACCGGCGGTAATAGCCAACCCAAAGCC‑CGCCAGGGTTGCGCTACCCACCCGCGCCG‑CCAAACTCGGACACAACGAC‑CGAAATGGAGTTAAACACCCCCGCTCTGGAGGCAAGGTA‑TTTTTTAG‑ACTTTTGGCAAACAGGCGGGTGg‑aga‑ga                                                                                                                          >  2:427037/1‑239 (MQ=255)

                                                                                                                      taAAGTTGTGCCAGAGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACAT‑CCAAAAGACGGCTCCAGGTCCACCGGCGGTAATAGCCAGCGCAACGCC‑GGCCAGGTTGCCGCTACCCACGCGCGCCG‑CAAGACTGGTACACAATGAC‑TGAAATGAGGTTAAACCGCCTGGCTGTGGATGAATGCTA‑TTTTTAAG‑ACTTTTGCCAAACTGGCGGATGT‑AGC‑Gaaa                                                                                                                        <  1:1288884/239‑1 (MQ=255)

                                                                                                                           ttGTGCCAGAGACAATCCGGCAAACGAGGGCGCCATGCCGATAAACGAGGCAACCCACAT‑CCAAAAGACGGTTCCAGGTCCACCGGCGGTAATAGCCAGCGCAACGCC‑GGCCAGGTTGCCGCTACCCACGCGCGCCG‑CAAGACTGGTACACAATGAC‑TGAAATGAGGTTAAACCGCCTGGCTGTGGATGAATGCTA‑TTTTTAAG‑ACTTTTGCCAAACTGGCGGATGT‑AGC‑GAAAC‑TGca                                                                                                                  <  1:1409775/239‑1 (MQ=255)

                                                                                                                              tgCCAGAGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACAT‑CCAAAAGACGGCTCCAGGTCCACCGGCGGTAATAGCCCGCGCAACGCC‑GGCCAGGGTGCCGCTACCCACCCGCGCCG‑CAAGACTGGTACACAATGAC‑TGAAATAAGGTTAAACCGCCTGGCTGTGGATGAAAGCTA‑TTTTTAAG‑ACTTTTTCCAAACTGGCGGGTGT‑AGA‑GAAAC‑TACACaa                                                                                                               >  2:471598/1‑239 (MQ=255)

                                                                                                                              tgCCAGAGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACAT‑CCAAAAGACGGCTCCAGGTCCACCGGCGGTAATAGCCAGCGCAACGCC‑GGCCCGGTTGGCGCTACCCACGCGCGCCG‑CAAGAATGGGACACAATGAC‑TGAAATGAGGTTAAACCGCCCGGCGGGGGGTGGATGCTATTTTTTAAG‑A‑TTTTGCCAAACTGGCGGAAGT‑ATG‑GAAAcctgacaa                                                                                                                >  2:799923/1‑232 (MQ=255)

                                                                                                                              tgCCAGAGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACAT‑CCAAAAGACGGCTCCAGGTCCACCGGCGGTAATAGCCAGCGCAACGCC‑GGCCCGGTTGCCGCTACCCCCGCGCGCCG‑CAAGACTGGGACAAAATGAC‑TGGAATGGGGGTAAAACGCCTGGCTGGGGGTGAATGCTA‑TTTTTAAA‑ACTTTTGCCAAACTCGCGGGTGG‑AGC‑Ggaaaacgacaa                                                                                                                >  2:188013/1‑231 (MQ=255)

                                                                                                                              tgCCAGAGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACAT‑CCAAAAGACGGCTCCAGGTCCACCGGCGGTAATAGCCAGCGCAACGCC‑GGCCCGGGTGGCGCTACCCACGCGCGCCG‑CAAAACTGGGACACAATTAC‑TGAAATGAGGTTAAACCGCCTGGCTGTGGGTGAATGCTA‑TTTTTAAG‑ACTTTTGCCAAACTGGCGGGAGT‑AGA‑GAAACTTGcaca                                                                                                                >  2:1238458/1‑239 (MQ=255)

                                                                                                                              tgCCAGAGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACAT‑CCAAAAGACGGCTCCAGGTCCACCGGCGGTAATAGCCAGCGCAACGCC‑GGCCAGGATGCCGCCACCCACCCGCCCCC‑CAAGAACTGTACACAATTAA‑TGAAAAGAGGGTAAACCACCTGGCTGGGGGTGAATGCTA‑TTTTTAAGAATTTTTGCCAAA‑TCGCGGAATT‑AGG‑GGAAA‑TGCACaa                                                                                                               >  2:908464/1‑239 (MQ=255)

                                                                                                                              tgCCAGAGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACAT‑CCAAAAGACGGCTCCAGGTCCACCGGCGGTAATAGCCAGCGCAACGCC‑CGCCCGGTTGCCGCTACCCACGCGCGCCG‑CAAGACTGGTACACAATGAC‑TGAAATGGGGGTTAACCGCCTGGCTGGGGATGGATGCTA‑TTTTTTAG‑ACTTTTGCCAAACCTGCGGGGGT‑AGC‑GGAAcctcacca                                                                                                                >  2:578997/1‑232 (MQ=255)

                                                                                                                                ccAGAGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACAT‑CCAAAAGACGGCTCCAGGTCCACCGGCGGTAATAGCCAGCGCAACGCC‑CGCCAGGGTGCCGCTACCCCCGCGCGCCC‑CCAGAGTGGTACACAAAGAC‑GGAAATTAGGGTAAACCCCCCGGGCGGGGATGAAAGGTA‑TTTTTTAG‑ACTTTTGCCCAAACGGCCGGTGT‑AGCGGAAAC‑TGACCaaa                                                                                                              >  2:1335365/1‑239 (MQ=255)

                                                                                                                                    agaACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACAT‑CCAAAAGACGGCTCCAGGTCCACCGGCGGCAATAACCCGCGCAACGCC‑GGCCCGGTTGCCGCTACCCACGCGCGCCG‑CCAGACTGGACCACACTGAC‑TGAAATGAGGTTAAACCGCCTGGCTGTGGATGAAAGGTC‑TTTTTAAG‑ACTTTTGCCAAACTGGCGGACGT‑AGG‑CGAAC‑CGCACAAAAcccg                                                                                                         >  2:275808/1‑237 (MQ=255)

                                                                                                                                     gaACATTCGGAAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACAT‑CCAAAAGACGGCTCCAGGTCCACCGGCGGTAATAGCCAGCGCAACGCC‑GGCCAGGTTGCCGCTACCCACGCGCGCCG‑CAAGACTGGTACACAATGAC‑TGAAATGAGGTTAAACCGCCTGGCTGTGGATGAATGCTA‑TTTTTAAG‑ACTTTTGCCAAACTGGCGGATGT‑AGC‑GAAAC‑TGCACAAATcc                                                                                                           <  1:1198640/236‑1 (MQ=255)

                                                                                                                                                aaaCGAGGTCGCCATGCCGATAAACGCGGCAACCCACAT‑CCAAAAGACGGCTCCAGGTCCACCGGCGGTAATAGCCAGCGCAACGCC‑GGCCAGGTTGCCGCTACCCACGCGCGCCG‑CAAGACTGGTACACAATGAC‑TGAAATGAGGTTAAACCGCCTGGCTGTGGATGAATGCTA‑TTTTTAAG‑ACTTTTGCCAAACTGGCGGATGT‑AGC‑GAAAC‑TGCACAAATCC‑GGTGCGAAAAGTg                                                                                             <  1:555033/238‑1 (MQ=255)

                                                                                                                                                aaaCGAGGTCGCCACGCCGATAAACGCGGCAACCCACAT‑CCAAAAGACGGCTCCAGGTCCACCGGCGGTAATAGCCAGCGCAACGCC‑GGCCAGGTTGCCGCTACCCACGCGCGCCG‑CAAGACTGGTACACAATGAC‑TGAAATGAGGTTAAACCGCCTGGCTGTGGATGAATGCTA‑TTTTTAAG‑ACTTTTGCCAAACTGGCGGATGT‑AGC‑GAAAC‑TGCACAAATCC‑GGTGCGAAAAGTg                                                                                             <  1:1301027/238‑1 (MQ=255)

                                                                                                                                                aaaCGAGGTCGCAATGCCGATAAACGCGGCAACCCACAT‑CCAAAAGACGGCTCCAGGTCCACCGGCGGTAATAGCCAGCGCAACGCC‑GGCCAGGTTGCCGCTACCCACGCGCGCCG‑CAAGACTGGTACACAATGAC‑TGAAATGAGGTTAAACCGCCTGGCTGTGGATGAATGCTA‑TTTTTAAG‑ACTTTTGCCAAACTGGCGGATGT‑AGC‑GAAAC‑TGCACAAATCC‑GGTGCGAAAAGTg                                                                                             <  2:330117/238‑1 (MQ=255)

                                                                                                                                                aaaCGAGGCCGCCATGCCGATAAACGCGGCAACCCACAC‑CCAAAAGACGGCTCCAGGTCCACCGGCGGTAATAGCCAGCGCAACGCC‑GGCCAGGTTGCCGCTACCCACGCGCGCCG‑CAAGACTGGTACACAATGAC‑TGAAATGAGGTTAAACCGCCTGGCTGTGGATGAATGCTA‑TTTTTAAG‑ACTTTTGCCAAACTGGCGGATGT‑AGC‑GAAAC‑TGCACAAATCC‑GGTGCGAAAAGTg                                                                                             <  1:1378946/238‑1 (MQ=255)

                                                                                                                                                 aaCGAGGCCGCCATGCCGATAAACGCGGCAACCCACAT‑CCAAAAGACGGCTCCAGGTCCACCGGCGGTAATAGCCAGCGCAACGCC‑GGCCAGGTTGCCGCTACCCACGCGCGCCG‑CAAGACTGGTACACAATGAC‑TGAAATGAGGTTAAACCGCCTGGCTGTGGATGAATGCTA‑TTTTTAAG‑ACTTTTGCCAAACTGGCGGATGT‑AGC‑GAAAC‑TGCACAAATCC‑GGTGCGAAAAGTGaa                                                                                           <  2:1093621/239‑1 (MQ=255)

                                                                                                                                                          gCCATGCCGATAAACGCGGCAACCCACAT‑CCAAAAGACGGCTCCAGGTCCACCGGCGGTAATAGCCAGCGCAACGCC‑GGCCAGGTTGCCGCTACCCACGCGCGCCG‑CAAGACTGGTACACAATGAC‑TGAAATGAGGTTAAACCGCCTGGCTGTGGATGAATGCTA‑TTTTTAAG‑ACTTTTGCCAAACTGGCGGATGT‑AGC‑GAAAC‑TGCACAAATCC‑GGTGCGAAAAGTGAACCaacaac                                                                                   <  1:427037/238‑1 (MQ=255)

                                                                                                                                                            cATGCCGAAAAACGCGGCAACCCACAC‑CCAAAAGACGGCCCCAGGTCCACCGGCGGTAATAGCCAGCGCAACCCC‑GGCCAGGTTGCCGCTACCCACGCGCGCCG‑CAAGACTGGTACACAATGAC‑TGAAATGAGGTTAAACCGCCTGGCTGTGGATGAATGCTA‑TTTTTAAG‑ACTTTTGCCAAACTGGCGGATGT‑AGC‑GAAAC‑TGCACAAATCC‑GGTGCGAAAAGTGAACCAACAACCTg                                                                                <  2:114147/239‑1 (MQ=255)

                                                                                                                                                              tGCCGATAAACGCGGCAACCCACAT‑CCAAAAGACGGCTCCAGGTCCACCGGCGGTAATAGCCAGCGCAACGCC‑CGCCAGGGTGCCGCCACCCCCGCGCGCCG‑CAAGACTGGTACACAATGAC‑TGAAATGAGGTTAAACCGCCCGGCTGTGGATGAATGCTA‑TTTTTTAG‑TCTTTTGTCCAACTTGGGGGTGTGAGG‑GAAAT‑TCCACAATACC‑GGGCGGAAAAGAGACCAAACAAccag                                                                                >  2:1001613/1‑236 (MQ=255)

                                                                                                                                                               gCCGATAAACGAGGCAACCCACAT‑CCAAAAGACGGCTCCAGGTCCACCGGCGGTAATAGCCAGCGCAACGCC‑GGCCAGGTGGCCGCTACCCACGCGCGCCG‑CAAGACTGGTACAAAATGAC‑TGAAATGAGGTTAAACCGCCTGGCTGTGGATGAATGCTA‑TTTTTAAG‑ACTTTTGCCAAACTGGCGGATGT‑AGC‑GAAAC‑TGCACAAATCC‑GGTGCGAAAAGTGAACCAACAACCTgcgc                                                                             <  1:1283720/239‑1 (MQ=255)

                                                                                                                                                                      aagcaggaaaCCCACAA‑ACAATAGATGGCTCCAGGTCTACCGCCGGTAACAGCCAGCGCAGAGCC‑GGCCAGGTTGCCATTCCCCCCGAGCGTCT‑CAGGATTGTTCCAAAAAGAC‑TAAAATGAGGTTAAACCGCCTGGCTATGGATGAATGCTA‑GTTTTAAG‑GCTTTTGCCAAACTGGCGGATGT‑AGC‑GAAAC‑TGCACAAATCC‑GGTGCGAAAAGTGAACCAACAACCTGCGCCGAagag                                                                      <  2:1369233/231‑1 (MQ=255)

                                                                                                                                                                      aaCGCGGCAACCCACAT‑CCAAAGAGCGCCGCCAGGTCCACCGGCGGTAATGGCCAGGGCAACGCC‑GGCCAGTTTGCCGCTGCGCGCGCGCGCCG‑CAAGACTGGTACACAATGAC‑TGAAATGAGGTTAAACCGCCTGGCTGTGGATGAATGCTA‑TTTTTAAG‑ACTTTTGGCAAACTGGCGGATGT‑AGC‑GAAAC‑TGCACAAATCC‑GGTGCGAAAAGTGAACCAACAACCTGCGCCGAagag                                                                      <  2:632448/239‑1 (MQ=255)

                                                                                                                                                                        cgcgGCAACCCACAT‑CCAAAAGACGGCTCCAGGTCCACCGGCGGTAATAGCCAGCGCAACGCC‑GGCCAGGTTGCCGCTACCCACGCGCGCCG‑CAAGACTGGGACACAATGAC‑TGAAATGAGGGTAAACCGCCTGGCTGTGGATGAATGCTA‑TTTTTAAG‑ACTTTTGCCAAACTGGCGGGTGT‑AGC‑GAAAA‑CGCACAAAACC‑GGGGCGAAAAAGGGACCAAAAACCTGCGCCGAAGAGCa                                                                    >  2:349259/1‑239 (MQ=255)

                                                                                                                                                                          cgGCAACCCACAT‑CCAAAAGCCGGCTCCAGGTCCACCGGCGGTAATAGCCAGCGCAACGCC‑GGCCAGGTTGCCGCTACCCACGCGCGCCG‑CAAGACTGGTACACAATGAC‑TGAAATGAGGTTAAACCGCCTGGCTGTGGATGAATGCTA‑TTTTTAAG‑ACTTTTGCCAAACTGGCGGATGT‑AGC‑GAAAC‑TGCACAAATCC‑GGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAgg                                                                  <  1:1238458/239‑1 (MQ=255)

                                                                                                                                                                          cgGCAACCCACAT‑CCAAAAGACGGCTCCAGGTCCACCGGCGGTAATAGCCAGGCCAACGCC‑GGCCAGGTTGCCGCTACCCACGCGCGCCG‑CAAGACTGGTACACAATGAC‑TGAAATGAGGTTAAACCGCCTGGCTGTGGATGAATGCTA‑TTTTTAAG‑ACTTTTGCCAAACTGGCGGATGT‑AGC‑GAAAC‑TGCACAAATCC‑GGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAgg                                                                  <  1:701177/239‑1 (MQ=255)

                                                                                                                                                                          cgGCAACCCACAT‑CCAAAAGACGGCTCCAGGTCCACCGGCGGTAATAACCAGCGCAACGCC‑GGCCAGGGTGCCGCCACCCCCGCGCGCCG‑CAAGACTGGGACACAATGAC‑TGAAATGAGGGTAAACCCCCCGGCTGTGGGTGAATGCTA‑TTTTTTAG‑ACTTTTGCCCAACTGGCGGGTGT‑AGC‑GAAAC‑TGCACAAATCC‑GGTGCGAAAAGTGAGACAACAAACCGCGCCCAAGAGCAgg                                                                  >  2:417790/1‑239 (MQ=255)

                                                                                                                                                                           ggCAACCCACATCCCAAAAGACGGCTCCAGGTCCACCGGCGGTAATAGCCAGCGCAACGCC‑GGCCAGGTTGCCGCTACCCACGCGCGCCG‑CAAGACTGGTACACAATGAC‑TGAAATGAGGTTAAACCGCCTGGCTGTGGATGAATGCTA‑TTTTTAAG‑ACTTTTGCCAAACTGGCGGATGT‑AGC‑GAAAC‑TGCACAAATCC‑GGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAgg                                                                  <  1:511917/239‑1 (MQ=255)

                                                                                                                                                                           ggCAACCCACAT‑CCAAAAGACGGCTCCAGGTCCACCGGCGGTAATAGCCAGCGCAACGCC‑GGCCAGGTTGCCGCTACCCACGCGCGCCG‑CAAGACTGGTACACAATGAC‑TGAAATGAGGTTAAACCGCCTGGCTGTGGATGAATGCTA‑TTTTTAAG‑ACTTTTGCCAAACTGGCGGATGT‑AGC‑GAAAC‑TGCACAAATCC‑GGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAgg                                                                  <  1:188013/238‑1 (MQ=255)

                                                                                                                                                                           ggCAACCCACAT‑CCAAAAGACGGCCCCAGGTCCACCGGCGGTAATAGCCAGCGCAACGCC‑GGCCAGGTTGCCGCTACCCACGCGCGCCG‑CAAGACTGGTACACAATGAC‑TGAAATGAGGTTAAACCGCCTGGCTGTGGATGAATGCTA‑TTTTTAAG‑ACTTTTGCCAAACTGGCGGATGT‑AGC‑GAAAC‑TGCACAAATCC‑GGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAgg                                                                  <  2:411698/238‑1 (MQ=255)

                                                                                                                                                                             cAACCCCCAT‑CCAAAAAACGGCACCAGGTCCCCCGGCGGTAATAGCAAGCGCAACGCC‑GGCCAGGTCGCCGCTCCCCACGCGCGCCG‑CAAGACTGATACACAATGAC‑TGAAATGAGGTTAAACCGCCTGGCTGTGGATGAATGCTA‑TTTTTAAG‑ACTTTTGCCAAACTGGCGGATGT‑AGC‑GAAAC‑TGCACAAATCC‑GGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTa                                                                <  1:908464/238‑1 (MQ=255)

                                                                                                                                                                                      t‑CCAAAAGACGGCTCCAGGTCCCCCGGCGGTAATAGCCAGCGCAACGCC‑GGCCAGGTTGCCGCTACCCACGCGCGCCG‑CAAGACTGGTACACAATGAC‑TGAAATGAGGTTAAACCGCCTGGCTGTGGATGAATGCTA‑TTTTTAAG‑ACTTTTGCCAAACTGGCGGATGT‑AGC‑GAAAC‑TGCACAAATCC‑GGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTAcc                                                      <  1:799923/239‑1 (MQ=255)

                                                                                                                                                                                            aaGACGGCTCCAGGTCCACCGGCGGTAATAGCCCGCGCAACGCC‑GGGCCGGGTGCCGCTACCCCCGCGCGCCG‑CACGACCGGTACACAAAGAC‑TGCAATGAGGGTAAACACCCTGGCCGTGGATGAATGCTA‑TTTTTAAG‑ACTTTTGCCAAACACGCGGGTGT‑GGC‑GGGAA‑TGGACAAAAAC‑GGAGCGACAAGTGAAACAACAACAGCCGCCCAAGAACAGGCAAATCAtactcgatc                                                  >  2:14494/1‑230 (MQ=255)

                                                                                                                                                                                                    gccAGGTCCCCCGGCGGTAATAGCCAGCGCAACGCC‑GGCCAGGTTGCCGCTACCCACGCGCGCCG‑CAAGACTGGTACACAATGAC‑TGAAATGAGGTTAAACCGCCTGGCTGTGGATGAATGCTA‑TTTTTAAG‑ACTTTTGCCAAACTGGCGGATGT‑AGC‑GAAAC‑TGCACAAATCC‑GGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAGGa                                         <  1:578997/238‑1 (MQ=255)

                                                                                                                                                                                                     ccAGGTCCACCGGCGGTAATAGCCAGCGCAACGCC‑GGCCAGGTTGCCGCTACCCACGCGCGCCG‑CAAGACTGGTACACAATGAC‑TGAAATGAGGTTAAACCGCCTGGCTGTGGATGAATGCTA‑TTTTTAAG‑ACTTTTGCCAAACTGGCGGATGT‑AGC‑GAAAC‑TGCACAAATCC‑GGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAGGAc                                        <  1:392518/239‑1 (MQ=255)

                                                                                                                                                                                                      cAGGTCCACCGGCGGTAATAGCCAGCGCAACGCC‑GGCCAGGTTGCCGCTACCCACGCGCGCCG‑CAAGACTGGTACACAATGAC‑TGAAATGAGGTTAAACCGCCTGGCTGTGGAt                                                                                                                                                                          >  1:361375/1‑114 (MQ=255)

                                                                                                                                                                                                      cAGGTCCACCGGCGGTAATAGCCAGCGCAACGCC‑GGCCAGGTTGCCGCTACCCACGCGCGCCG‑CAAGACTGGTACACAATGAC‑TGAAATGAGGTTAAACCGCCTGGCTGTGGAt                                                                                                                                                                          <  2:361375/114‑1 (MQ=255)

                                                                                                                                                                                                      cAGGGCCGCCGGCGGAAAAAGCCAGCGCAACGCC‑GGCCAGGGTGCCGCTACCCAAGCGCGCCG‑CAAGACTGGTACACAATGAC‑TGAAAAGAGGTTAAACCGCCTGGCTGTGGATGAATGCTA‑TTTTTAAG‑ACTTTTGCCAAACTGGCGGATGT‑AGC‑GAAAC‑TGCACAAATCC‑GGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAGGACg                                       <  1:716656/239‑1 (MQ=255)

                                                                                                                                                                                                      cAGGCCCACCGGCGGTAATACCCAGCGCAACGCC‑GGCAAGGTTGCCACTACCCACGCGCGCCG‑CAAGACTGGTACACAATGAC‑TGAAATGAGGTTAAACCGCCTGGCTGTGGATGAATGCTA‑TTTTTAAG‑ACTTTTGCCAAACTGGCGGATGT‑AGC‑GAAAC‑TGCACAAAGCC‑GGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAGGACg                                       <  1:1086074/239‑1 (MQ=255)

                                                                                                                                                                                                        ggTCCACCGGCGGTAATAGCCAGCGCAACGCC‑GGCCAGGGTGCCGCTACCCACGCGCGCCG‑CAAGACTGGTACACAATGAC‑TGAAATGAGGGTGAACCGCCCGGCTGTGGGTGAATGCTA‑TTTTTTAG‑ACTTTTGCCAAAATGGCGGCGGT‑AGG‑GAAAA‑AGCACAAAACCGGGTCCAGAAAGGAAACAAACAACCT‑CCCCCAAGAGCCGGGGAATCATTTACGATTCCCAAAGGCCGcc                                     >  2:1067201/1‑238 (MQ=255)

                                                                                                                                                                                                                   ggTAATAGCCAGCGCAACGCC‑GGCCAGGTTGCCGCTACCCACGCGCGCCG‑CAAGACTGGTACACAATGAC‑TGAAATGAGGTTAAACCGCCTGGCTGTGGATGAATGCTA‑TTTTTAAG‑ACTTTTGCCAAACTGGCGGATGT‑AGC‑GAAAC‑TGCACAAATCC‑GGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAGGACGCTGTTAATGAAg                           <  1:229462/238‑1 (MQ=255)

                                                                                                                                                                                                                               cgcAACGCC‑GGCCAGGTTGCCGCTACCCACGCGCGCCG‑CAAGACTGGTACACAATGAC‑TGAAATGAGGTTAAACCGCCTGGCTGTGGATGAATGCTA‑TTTTTAAG‑ACTTTTGCCAAACTGGCGGATGT‑AGC‑GAAAC‑TGCACAAATCC‑GGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAGGACg                                       >  1:324118/1‑214 (MQ=255)

                                                                                                                                                                                                                               cgcAACGCC‑GGCCAGGTTGCCGCTACCCACGCGCGCCG‑CAAGACTGGTACACAATGAC‑TGAAATGAGGTTAAACCGCCTGGCTGTGGATGAATGCTA‑TTTTTAAG‑ACTTTTGCCAAACTGGCGGATGT‑AGC‑GAAAC‑TGCACAAATCC‑GGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAGGACg                                       <  2:324118/214‑1 (MQ=255)

                                                                                                                                                                                                                               cgcAACGCC‑GGCCAGGTTGCCGCTACCCACGCGCGCCG‑CAAGACTGGTACACAATGAC‑TGAAATGAGGTTAAACCGCCTGGCTGTGGATGAATGCTA‑TTTTTAAG‑ACTTTTGCCAAACTGGCGGATGT‑AGC‑GAAAC‑TGCACAAATCC‑GGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAGGACGCTGTTAATGAAGGAGAAAAAATCTg              >  1:63534/1‑239 (MQ=255)

                                                                                                                                                                                                                               cgcAACGCC‑GGCCAGGTTGCCGCTACCCACGCGCGCCG‑CAAGACTGGTACACAATGAC‑TGAAATGAGGTTAAACCGCCTGGCTGTGGATGAATGCTA‑TTTTTAAG‑ACTTTTGCCAAACTGGCGGATGT‑AGC‑GAAAC‑TGCACAAATCC‑GGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAGGACGCTGTTAATGAAGGAGAAAAAATCTg              >  2:54068/1‑239 (MQ=255)

                                                                                                                                                                                                                               cgcAACGCC‑GGCCAGGTTGCCGCTACCCACGCGCGCCG‑CAAGACTGGTACACAATGAC‑TGAAATGAGGTTAAACCGCCTGGCTGTGGATGAATGCTA‑TTTTTAAG‑ACTTTTGCCAAACTGGCGGATGT‑AGC‑GAAAC‑TGCACAAATCC‑GGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAGGACGCTGTTAATGAAGGAGAAAAAATCTg              >  1:782632/1‑239 (MQ=255)

                                                                                                                                                                                                                               cgcAACGCC‑GGCCAGGTTGCCGCTACCCACGCGCGCCG‑CAAGACTGGTACACAATGAC‑TGAAATGAGGTTAAACCGCCTGGCTGTGGATGAATGCTA‑TTTTTAAG‑ACTTTTGCCAAACTGGCGGATGT‑AGC‑GAAAC‑TGCACAAATCC‑GGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAGGACGCTGTTAATGAAGGAGAAAAAATCTg              >  2:29762/1‑239 (MQ=255)

                                                                                                                                                                                                                               cgcAACGCC‑GGCCAGGTTGCCGCTACCCACGCGCGCCG‑CAAGACTGGTACACAATGAC‑TGAAATGAGGTTAAACCGCCTGGCTGTGGATGAATGCTA‑TTTTTAAG‑ACTTTTGCCAAACTGGCGGATGT‑AGC‑GAAAC‑TGCACAAATCC‑GGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAGGACGCTGTTAATGAAGGAGAAAAAATCTg              >  2:260541/1‑239 (MQ=255)

                                                                                                                                                                                                                                ccagcccc‑GGCCAGGTGCCCGCTACCCACGCGCGCCG‑CAAGACTGGTACACAATGAC‑TGAAATGAGGTTAAACCGCCTGGCTGTGGATGAATGCTA‑TTTTTAAG‑ACTTTTGCCAAACTGGCGGATGT‑AGC‑GAAAC‑TGCACAAATCC‑GGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAGGACGCTGTTAATGAAGGAGAAAAAATCTgg             <  2:1108594/233‑1 (MQ=255)

                                                                                                                                                                                                                                  aaCGCC‑GGCCAGGTTGCCGCTACCCACGCGCGCCG‑CAAGACTGGTACACAATGAC‑TGAAATGAGGTTAAACCGCCTGGCTGTGGATGAATGCTA‑TTTTTAAG‑ACTTTTGCCAAACTGGCGGATGT‑AGC‑GAAAC‑TGCACAAATCC‑GGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAGGACGCTGTTAATGAAGGAGAAAAAATCTGgc            <  1:1044719/238‑1 (MQ=255)

                                                                                                                                                                                                                                  aaCGCC‑GGCCAGGTTGCCGATACCCACGCGCGCCG‑CAAGACTGGTACACAATGAC‑TGAAATGAGGTTAAACCGCCTGGCTGTGGATGAATGCTA‑TTTTTAAG‑ACTTTTGCCAAACTGGCGGATGT‑AGC‑GAAAC‑TGCACAAATCC‑GGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAGGACGCTGTTAATGAAGGAGAAAAAATCTGgc            <  1:1067201/238‑1 (MQ=255)

                                                                                                                                                                                                                                    cGCC‑GGCCAGGTTGCCGCTACCCACGCGCGCCG‑CAAGACTGGTACACAATGAC‑TGAAATGAGGTTAAACCGCCTGGCTGTGGATGAATGCTA‑TTTTTAAG‑ACTTTTGCCAAACTGGCGGATGT‑AGC‑GAAAC‑TGCACAAATCC‑GGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAGGACGCTGTTAATGAAGGAGAAAAAATCTGgcat          >  2:1295524/1‑238 (MQ=255)

                                                                                                                                                                                                                                    cGCC‑GGCCAGGTTGCCGCTACCCACGCGCGCCG‑CAAGACTGGTACACAATGAC‑TGAAATGAGGTTAAACCGCCTGGCTGTGGATGAATGCTA‑TTTTTAAG‑ACTTTTGCCAAACTGGCGGATGT‑AGC‑GAAAC‑TGCACAAATCC‑GGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAGGACGCTGTTAATGAAGGAGAAAAAATCTGgcat          >  1:121533/1‑238 (MQ=255)

                                                                                                                                                                                                                                          gCCAGGTTGCCGCTACCCACGCGCGCCG‑CAAGACTGGTACACAATGAC‑TGAAATGAGGTTAAACCGCCTGGCTGTGGATGAATGCTA‑TTTTTAAG‑ACTTTTGCCAAACTGGCGGATGT‑AGC‑GAAAC‑TGCACAAATCC‑GGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAGGACGCTGTTAATGAAGGAGAAAAAATCTGGCATGCata     >  2:715338/1‑238 (MQ=255)

                                                                                                                                                                                                                                          gCCAGGTTGCCGCTACCCACGCGCGCCG‑CAAGACTGGTACACAATGAC‑TGAAATGAGGTTAAACCGCCTGGCTGTGGATGAATGCTA‑TTTTTAAG‑ACTTTTGCCAAACTGGCGGATGT‑AGC‑GAAAC‑TGCACAAATCC‑GGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGAACCCCAAAGGACGCTGTTAATGAAGGAGAAACAATCAGGCAAGCata     >  2:115747/1‑238 (MQ=255)

                                                                                                                                                                                                                                            cAGGTTGCCGCTACCCACGCGCGCCG‑CAAGACTGGTACACAATGAC‑TGAAATGAGGTTAAACCGCCTGGCTGTGGATGAATGCTA‑TTTTTAAG‑ACTTTTGCCAAACTGGCGGATGT‑AGC‑GAAAC‑TGCACAAATCC‑GGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAGGACGCTGTTAATGAAGGAGAAAAAATCTGGCATGCATATcc  <  1:284720/239‑1 (MQ=255)

GCCATAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGACGTCACGTTCTTTATAAAGTTGTGCCAGAGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACAT‑CCAAAAGACGGCTCCAGGTCCACCGGCGGTAATAGCCAGCGCAACGCC‑GGCCAGGTTGCCGCTACCCACGCGCGCCG‑CAAGACTGGTACACAATGAC‑TGAAATGAGGTTAAACCGCCTGGCTGTGGATGAATGCTA‑TTTTTAAG‑ACTTTTGCCAAACTGGCGGATGT‑AGC‑GAAAC‑TGCACAAATCC‑GGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAGGACGCTGTTAATGAAGGAGAAAAAATCTGGCATGCATATCC  >  NC_000913/7495‑7967

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`