BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	3,153,892	T→C	12.8%	Y110Y (TAT→TAC)	yghB →	general envelope maintenance protein; DedA family inner membrane protein; putative multidrug efflux transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,153,892	0	T	C	12.8%	330.5 / 2.7	117	Y110Y (TAT→TAC)	yghB	general envelope maintenance protein; DedA family inner membrane protein; putative multidrug efflux transporter
Reads supporting (aligned to +/- strand): ref base T (62/35); new base C (2/16); total (65/52)
Fisher's exact test for biased strand distribution p-value = 4.01e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 117 total aligned reads displayed.

CATGTTTGCCACGCTGTTTTTAG‑AAAACGGCCTGCTGCCCGCCTCATTTTTGCCAGGCGACAGCTTGTTGATACTGGCAGGCGCATTGATTGCCCAGGGGGTTATGGATTTTCTGCCTACGATTGCGATTCTGA‑CCGCCGCAGCAAGTCTGGGCTGCTGGCTAAGTTATATTCAGGGGCGCTG‑GTTAGGG‑AATACC‑AAAA‑CGGTGAAAGGCT‑GGCTGGCA‑CAGCTTCCTGCTAAATAT‑CACCAGCG‑CGCCACCTGCA‑TGTTTGACCGCCACGGTCTGCTGGCGCTGCTGGCTGGACGTTTTCTTGCATTTGTCCGTACGCTGCTGCCAACCATGGCGGGAATTTCCGGTCTGCCAAACCGC‑CGCTTCCAGTTTTTCAACTGGTTAAGTGGATTGCTGTGGGTCAGCGTGGTAACCAGTTTTGGCTATGCCTTAAGTATGATTCCGTTCGTTAAACGCCATGAAGATCAGGTA  >  NC_000913/3153655‑3154126

cATGTTTGCCACGCTGTTTTTAG‑AAAACGGCCTGCTGCCCGCCTCATTTTTGCCAGGCGACAGCTTGTTGATACTGGCAGGCGCATTGATTGCCCAGGGGGTTATGGATTTTCTGCCTACGATTGCGATTCTGA‑CCGCCGCAGCAAGTCTGGGCTGCTGGCTAAGTTATATTCAGGGGCGCTG‑GTTAGGG‑AATACC‑AAAA‑CGGTGAAAGGCT‑GGCTGGCA‑CAGCTTCCTGCTAAATAT‑c                                                                                                                                                                                                                                              >  1:591996/1‑239 (MQ=255)

       gCCACGTTGTTTTTAG‑AAAACGGCCTGCTCCCCGCCTCATTTTTGCCAGGCGACAGCTTGTTGATACTGGCAGGCGCATTGATTGCCCGGGGGGTTATGGATTTTCTGCCTACGATTGCGATTCTGA‑CCGCCGCAGCAAGTCTGGGCTGCTGGCTAAGTTATATTCAGGGGCGCTG‑GTTAGGG‑AATACC‑AAAA‑CGGTGAAAGGCT‑GGCTGGCA‑CAGCTTCCTGCTAAATAT‑CACCAgcg                                                                                                                                                                                                                                       <  2:490076/239‑1 (MQ=255)

         cACGCTGTTTTTAG‑AAAACGGCCTGCTGCCCGCCTCATTTTTGCCAGGCGACAGCTTGTTGATACTGGCAGGCGCATTGATTGCCCAGGGGGTTATGGATTTTCTGCCTACGATTGCGATTCTGA‑CCGCCGCGGCAAGTCTGGGCTGCTGGCTAAGTTATATTCAGGGGCGCTG‑GTTAGGG‑AATACC‑AAAA‑CGGTGAAAGGCT‑GGCTGGCA‑CAGCTTCCTGTCAAATAT‑CACCAgacc                                                                                                                                                                                                                                      >  2:234769/1‑235 (MQ=255)

          aCGCTGTTTTTAG‑AAAACGGCCTGCTGCCCGCCTCATTTTTGCCAGGCGACAGCTTGTTGATACTGGCAGGCGCATTGATTGCCCAGGGGGTTATGGATTTTCTGCCTACGATTGCGATTCTGA‑CCGCCGCAGCAAGTCTGGGCTGCTGGCTAAGTTATATTCAGGGGCGCTG‑GTTAGGG‑AATACC‑AAAA‑CGGTGAAAGGCT‑GGCTGGCA‑CAGCTTCCTGCTAAATAT‑CACCAgcg‑cgc                                                                                                                                                                                                                                   <  1:1210988/239‑1 (MQ=255)

             ggttttttAGAAAAAGGGCCTGCGGCCCGCTTGATTTTTGCCAGGCGAAAGGTTGTTGATTCTGGCAGGCGCTTTGATTGCCCAGGGGGTTGTGGATTTTCTGCTTCCGATTGCGATTCTGA‑CCGCCGCAGCAAGTCTGGGCTGCTGGCTAAGTTATATTCAGGGGCGCTG‑GTTAGGG‑AATACC‑AAAA‑CGGTGAAAGGCT‑GGCTGGCA‑CAGCTTCCTGCTAAATAT‑CACCAGCG‑CGcc                                                                                                                                                                                                                                  <  2:181136/235‑1 (MQ=255)

                           aCGGCCTGCTGCCCGCCTCATTTTTGCCAGGCGACAGCTTGTTGATACTGGCAGGCGCATTGATTGCCCAGGGGGTTATGGATTTTCTGCCTACGATTGCGATTCTGA‑CCGCCGCAGCAAGTCTGGGCTGCTGGCTAAGTTATATTCAGGGGCGCTG‑GTTAGGG‑AATACC‑AAAA‑CGGTGAAAGGCT‑GGCTGGCA‑CAGCTTCCTGCTAAATAT‑CACCAGCG‑CGCCACCCGCA‑TGTTTGa                                                                                                                                                                                                                   >  2:357979/1‑238 (MQ=255)

                                ctgcgccccGCGTCATTTTGCCCGGCCGCAAGTTTGTTGATACTGGCGGCCGCATTGATTGCCCGGGGGTTTAGGGTTTTTCTGCCTACGATTGCGTTTCGGC‑CCGCCGCAGCAAGTCTGGGCTGCTGGCTAAGTTATATTCAGGGGCGCTG‑GTTAGGG‑AATACC‑AAAA‑CGGTGAAAGGCT‑GGCTGGCA‑CAGCTTCCTGCTAAATAT‑CACCAGCG‑CGCCACCTGCA‑TGTTTGACCGCCa                                                                                                                                                                                                             <  2:1050453/233‑1 (MQ=255)

                                  gctgcCCGCCTCATTTTTGCCAGGCGACAGCTTGTTGATACTGGCAGGCGCATTGATTGCCCAGGGGGTTATGGATTTTCTGCCTACGATTGCGATTCTGA‑CCGCCGCAGCAAGTCTGGGCTGCTGGCTAAGTTATATTCAGGGGCGCTG‑GTTAGGG‑AATACC‑AAAA‑CGGTGAAAGGCT‑GGCTGGCA‑CAGCTTCCTGCTAAATAT‑CACCAGCG‑CGCCACCTGCC‑TGTTTGACCGCCACg                                                                                                                                                                                                           >  1:826587/1‑239 (MQ=255)

                                         gCCTCATTTTTGCCAGGCGACAGCTTGTTGATACTGGCAGGCGCATTGATTGCCCAGGGGGTTATGGCTTTTCTGCCTACGATTGCGATTCTGA‑CCGCCGCAGCAAGTGTGGCCCTCTTGCTAAGTTATATTCAGGGGCGCTG‑GTTAGGG‑AATACC‑AAAA‑CGGTGAAAAGCT‑GGCTGGCA‑CAGCTGCCTTCTAAATCA‑CAccc                                                                                                                                                                                                                                          >  1:79505/1‑202 (MQ=255)

                                                     cccggCGACGGGTTGTTGATACGGGGAGGCGGTTTGTTTGCCCGGGGGGTTGGGGATTTTCTGCCTACGATGGCGATTGTGA‑CCGCCGCAGCAAGTCTGGGCTGCTGGCTAAGTTATATTCAGGGGCGCTG‑GTTAGGG‑AATACC‑AAAA‑CGGTGAAAGGCT‑GGCTGGCA‑CAGCTTCCTGCTAAATAT‑CACCAGCG‑CGCCACCTGCA‑TGTTTGACCGCCACGGTCTGCTGGCGCTgctgg                                                                                                                                                                                         <  2:483552/235‑1 (MQ=255)

                                                          cGACAGCTTGTTGATACTGGCAGGCGCATTGATTGCCCAGGGGGTTATGGATTTTCTGCCTACGATTGCGATTCTGA‑CCGCCGCAGCAAGTCTGGGCTGCTGGCTAAGTTATATTCAGGGGCGCTG‑GTTAGGG‑AATACC‑AAAA‑CGGTGAAAGGCT‑GGCTGGCA‑CAGCTTCCTGCTAAATAT‑CACCAGCG‑CGCCACCTGCA‑TGTTTGACCGCCACGGTCTGCTGGCGCTGCTGGCTGGAc                                                                                                                                                                                   >  2:235787/1‑239 (MQ=255)

                                                                     ggactgtGGGAGGGGCAGTGATTGCCCGGGGGTTTATGGATTTTCTGCTTACGAGGGGGATTCGGA‑CCGCCGCAGAAAGTCTGGGCTGCGGGCTAAGTTATATTCAGGGGCGCTG‑GTTAGGG‑AATACC‑AAAA‑CGGTGAAAGGCT‑GGCTGGCA‑CAGCTTCCTGCTAAATAC‑CACCAGCG‑CGCCACCTGCA‑TGTTTGACCGCCACGGTCTGCTGGCGCTGCTGGCTGGACGTTTTCTTGCa                                                                                                                                                                        <  2:817307/233‑1 (MQ=255)

                                                                             gCAGGCGCATTGATTGCCCAGGGGGTTATGGATTTTCTGCCTACGATTGCGATTCTGA‑CCGCCGCAGCAAGTCTGGGCTGCTGGCTAAGTTATATTCAGGGGCGCTG‑GTTAGGG‑AATACC‑AAAA‑CGGTGAAAGGCT‑GGCTGGCA‑CAGCTTCCTGCTAAATAT‑CACCAGCG‑CGCCACCTGCA‑TGTTTGACCGCCACGGTCTGCTGGCGCTGCTGGCTGGACGTTTTCTTGCATTTGTCCg                                                                                                                                                                >  2:1359118/1‑239 (MQ=255)

                                                                              cAGGCGCATTGATTGCCCAGGGGGTTATGGATTTTCTGCCTACGATTGCGATTCTGA‑CCGCCGCAGCAAGTCTGGGCTGCTGGCTAAGTTATATTCAGGGGCGCTG‑GTTAGGG‑AATACC‑AAAA‑CGGTGAAAGGCT‑GGCTGGCA‑CAGCTTCCTGCTAAATAT‑CACCAGCG‑CGCCACCTGCA‑TGTTTGACCGCCACGGTCTGCTGGCGCTGCTGGCTGGACGTTTTCTTGCATTTGTCCg                                                                                                                                                                <  1:96400/238‑1 (MQ=255)

                                                                              cAGGCGCATTGATTGCCCAGGGGGTTATGGATTTTCTGCCTACGATTGCGATTCTGA‑CCGCCGCAGCAAGTCTGGGCTGCTGGCTAAGTTATATTCAGGGGCGCTG‑GTTAGGG‑AATACC‑AAAA‑CGGTGAAAGGCT‑GGCTGGCA‑CAGCTTCCTGCTAAATAT‑CACCAGCG‑CGCCACCTGCA‑TGTTTGACCGCCACGGTCTGCTGGCGCTGCTGGCTGGACGTTTTCTTGCATTTGTCCGt                                                                                                                                                               >  2:123119/1‑239 (MQ=255)

                                                                                  cgcATTGATTGCCCAGGGGGTTATGGATTTTCTGCCTACGATTGCGATTCTGA‑CCGCCGCAGCAAGTCTGGGCTGCTGGCTAAGTTATATTCAGGGGCGCTG‑GTTAGGG‑AATACC‑AAAA‑CGGTGAAAGGCT‑GGCTGGCA‑CCGCTTCCTGCTAAATAT‑CACCAGCG‑CGCCACCTTCA‑TGTTTTACCGCCCCGGTCTGCCGGCGCTGCTGGCTTGCCGTTTTCTTGCATTTTTCCGTACg                                                                                                                                                            >  2:828701/1‑238 (MQ=255)

                                                                                    cATGGATTGCCCGGGGGGTTATGGTTTTTCGTCCTACGATTGCGTTTCTGA‑CCGCCGCAGCAAGTCTGGGCTGCTGGCTAAGTTATATTCAGGGGCGCTG‑GTTAGGG‑AATACC‑AAAA‑CGGTGAAAGGCT‑GGCTGGCA‑CAGCTTCCTGCTAAATAT‑CACCAGCG‑CGCCACCTGCA‑TGTTTGACCGCCACGGTCTGCTGGCGCTGCTGGCTGGACGTTTTCTTGCATTTGTCCGTACgctg                                                                                                                                                         <  2:696881/239‑1 (MQ=255)

                                                                                     attgattgCCCAGGGGGTTATGGATTTTCTGCCTACGATTGCGATTCTGA‑CCGCCGCAGCAAGTCTGGGCTGCTGGCTAAGTTATATTCAGGGGCGCTG‑GTTAGGG‑AATACC‑AAAA‑CGGTGAAAGGCT‑GGCTGGCA‑CAGCTTCCTGCTAAATAT‑CACCAGCG‑CGCCACCTGCA‑TGTTTGACCGCCACGGTCTGCTGGCGCTGCTGGCTGGACGTTTTCTTGCATTTGTCCGTACgct                                                                                                                                                          >  1:1248484/1‑237 (MQ=255)

                                                                                       tgattgCCCAGGGGGTTATGGATTTTCTGCCTACGATTGCGATTCTGA‑CCGCCGCAGCAAGTCTGGGCTGCTGGCTAAGTTATATTCAGGGGCGCTG‑GTTAGGG‑AATACC‑AAAA‑CGGTGAAAGGCT‑GGCTGGCA‑CAGCTTCCTGCTAAATAT‑CACCAGCG‑CGCCACCTGCA‑TGTTTGACCGCCACGGTCTGCTGGCGCTGCTGGCTGGACGTTTTCTTGCATTTGTCCGTACgctgct                                                                                                                                                       >  2:1071573/1‑238 (MQ=255)

                                                                                           tgCCCAGGGGGTTATGGATTTTCTGCCTACGATTGCGATTCTGA‑CCGCCGCAGCAAGTCTGGGCTGCTGGCTAAGTTATATTCAGGGGCGCTG‑GTTAGGG‑AATACC‑AAAA‑CGGTGAAAGGCT‑GGCTGGCA‑CAGCTTCCTGCTAAATAT‑CACCAGCG‑CGCCACCTGCA‑TGTTTGACCGCCACGGTCTGCTGGCGCTGCTGGCTGGACGTTTTCTTGCATTTGTCCGTACGCTGCTGCCa                                                                                                                                                   >  1:1275168/1‑238 (MQ=255)

                                                                                             cccAGGGGGTTATGGTTTTTCGGCCTCCGTTTGCG‑TTCCGACCCGCCGCAGCAAGTCTGGGCTGCTGGCTAAGTTATATTCAGGGGCGCTG‑GTTAGGG‑AATACC‑AAAA‑CGGTGAAAGGCT‑GGCTGGCA‑CAGCTTCCTGCTAAATAT‑CACCAGCG‑CGCCACCTGCA‑TGTTTGACCGCCACGGTCTGCTGGCGCTGCTGGCTGGACGTTTTCTTGCATTTGTCCGTACGCTGCTGCCAAc                                                                                                                                                 <  2:402780/238‑1 (MQ=255)

                                                                                                      ttATGGATTTTCTGCCTACGATTGCGATTCTGA‑CCGCCGCAGCAAGTCTGGGCTGCTGGCTAAGTTATATTCAGGGGCGCTG‑GTTAGGG‑AATACC‑AAAA‑CGGTGAAAGGCT‑GGCTGGCA‑CAGCTTCCTGCTAAATAT‑CACCAGCG‑CGCCACCTGCA‑TGTTTGACCGCCACGGTCTGCTGGCGCTGCTGGCTGGACGTTTTCTTGCATTTGTCCGTACGCTGCTGCCAACCATGGCggg                                                                                                                                        <  1:235787/238‑1 (MQ=255)

                                                                                                      ttATGGATTTCCTGCCTACGTTTGCGATTCTGA‑CCGCCGCAGCAAGTCTGGGCTGCTGGCTAAGTTATATTCAGGGGCGCTG‑GTTAGGG‑AATACC‑AAAA‑CGGTGAAAGGCT‑GGCTGGCA‑CAGCTTCCTGCTAAATAT‑CACCAGCG‑CGCCACCTGCA‑TGTTTGACCGCCACGGTCTGCTGGCGCTGCTGGCTGGACGTTTTCTTGCATTTGTCCGTACGCTGCTGCCAACCATGGCggg                                                                                                                                        <  1:1313987/238‑1 (MQ=255)

                                                                                                       tATGGATTTTCTGCCTACGATTGCGATTCTGA‑CCGCCGCAGCAAGTCTGGGCTGCTGGCTAAGTTATATTCAGGGGCGCTG‑GTTAGGG‑AATACC‑AAAA‑CGGTGAAAGGCT‑GGCTGGCA‑CAGCTTCCTGCTAAATAT‑CACCAGCG‑CGCCACCTGCA‑TGTTTGACCGCCACGGTCTGCTGGCGCTGCTGGCTGGACGTTTTCTTGCATTTGTCCGTa                                                                                                                                                              >  1:509438/1‑215 (MQ=255)

                                                                                                       tATGGATTTTCTGCCTACGATTGCGATTCTGA‑CCGCCGCAGCAAGTCTGGGCTGCTGGCTAAGTTATATTCAGGGGCGCTG‑GTTAGGG‑AATACC‑AAAA‑CGGTGAAAGGCT‑GGCTGGCA‑CAGCTTCCTGCTAAATAT‑CACCAGCG‑CGCCACCTGCA‑TGTTTGACCGCCACGGTCTGCTGGCGCTGCTGGCTGGACGTTTTCTTGCATTTGTCCGTACGCTGCTGCCCACCATGGCgggga                                                                                                                                      >  2:452495/1‑237 (MQ=255)

                                                                                                       tATGGATTTTCTGCCTACGATTGCGATTCTGA‑CCGCCGCAGCAAGTCTGGGCTGCTGGCTAAGTTATATTCAGGGGCGCTG‑GTTAGGG‑AATACC‑AAAA‑CGGTGAAAGGCT‑GGCTGGCA‑CAGCTTCCTGCTAAATAT‑CACCAGCG‑CGCCACCTGCA‑TGTTTGACCGCCACGGTCTGCTGGCGCTGCTGGCTGGACGTTTTCTTGCATTTGTCCGTACGCTGCTGCCAACCATGGCGGGaa                                                                                                                                      >  1:943212/1‑239 (MQ=255)

                                                                                                       tATGGATTTTCTGCCTACGATTGCGATTCTGA‑CCGCCGCAGCAAGTCTGGGCTGCTGGCTAAGTTATATTCAGGGGCGCTG‑GTTAGGG‑AATACC‑AAAA‑CGGTGAAAGGCT‑GGCTGGCA‑CAGCTTCCTGCTAAATAT‑CACCAGCG‑CGCCACCTGCA‑TGTTTGACCGCCACGGTCTGCTGGCGCTGCTGGCTGGACGTTTTCTTGCATTTGTCCGTACGCTGCTGCCAACCATGGCGGGaa                                                                                                                                      >  1:544883/1‑239 (MQ=255)

                                                                                                       tATGGATTTTCTGCCTACGATTGCGATTCTGA‑CCGCCGCAGCAAGTCTGGGCTGCTGGCTAAGTTATATTCAGGGGCGCTG‑GTTAGGG‑AATACC‑AAAA‑CGGTGAAAGGCT‑GGCTGGCA‑CAGCTTCCTGCTAAATAT‑CACCAGCG‑CGCCACCTGCA‑TGTTTGACCGCCACGGTCTGCTGGCGCTGCTGGCTGGACGTTTTCTTGCATTTGTCCGTACGCTGCTGCCAACCATGGCGGGaa                                                                                                                                      >  1:1197576/1‑239 (MQ=255)

                                                                                                        aTGGATTTTCTGCCTACGATTGCGATTCTGA‑CCGCCGCAGCAAGTCTGGGCTGCTGGCTAAGTTATATTCAGGGGCGCTG‑GTTAGGG‑AATACC‑AAAA‑CGGTGAAAGGCT‑GGCTGGCA‑CAGCTTCCTGCTAAATAT‑CACCAGCG‑CGCCACCTGCA‑TGTTTGACCGCCACGGTCTGCTGGCGCTGCTGGCTGGACGTTTTCTTGCAttt                                                                                                                                                                     <  1:330363/207‑1 (MQ=255)

                                                                                                        aTGGATTTTCTGCCTACGATTGCGATTCTGA‑CCGCCGCAGCAAGTCTGGGCTGCTGGCTAAGTTATATTCAGGGGCGCTG‑GTTAGGG‑AATACC‑AAAA‑CGGTGAAAGGCT‑GGCTGGCA‑CAGCTTCCTGCTAAATAT‑CACCAGCG‑CGCCACCTGCA‑TGTTTGACCGCCACGGTCTGCTGGCGCTGCTGGCTGGACGTTTTCTTGCAttt                                                                                                                                                                     >  2:330363/1‑207 (MQ=255)

                                                                                                            aTTTTCTGCCTACGATTGCGATTCTGA‑CCGCCGCAGCAAGTCTGGGCTGCTGGCTAAGTTATATTCAGGGGCGCTG‑GTTAGGG‑AATACC‑AAAA‑CGGTGAAAGGCT‑GGCTGGCA‑CAGCTTCCTGCTAAATAT‑CACCAGCG‑CGCCACCTGCA‑TGTTTGACCGCCACGGTCTGCTGGCGCTGCTGGCTGGACGTTTTCTTGCATTTGTCCGTACGCTGCTGCCAACCATGGCGGGGAttttc                                                                                                                                 >  1:653866/1‑237 (MQ=255)

                                                                                                              tttCTGCCTACGATTGCGATTCTGA‑CCGCCGCAGCAAGTCTGGGCTGCTGGCTAAGTTATATTCAGGGGCGCTG‑GTTAGGG‑AATACC‑AAAA‑CGGTGAAAGGCT‑GGCTGGCA‑CAGCTTCCTGCTAAATAT‑CACCAGCG‑CGCCACCTGCA‑TGTTTGACCGCCACGGTCTGCTGGCGCTGCTGGCTGGACGTTTTCTTGCATTTGTCCGTACGCTGCTGCCAACCATGGCGGGAATTTCCgg                                                                                                                               >  1:1139761/1‑239 (MQ=255)

                                                                                                                tttGCCTACGATTGCGATTCTGA‑CCGCCGCAGCAAGTCTGGGCTGCTGGCTAAGTTATATTCAGGGGCGCTG‑GTTAGGG‑AATACC‑AAAA‑CGGTGAAAGGCT‑GGCTGGCA‑CAGCTTCCTGCTAAATAT‑CACCAGCG‑CGCAACCTGCA‑TGTTTGACCGCCACGGTCTGCTGGCGCTGCTGGCTGGACGTCTTCTTGCATTTGTCCGTACGCTGCTGCCACCCCTGGCGGGacgtttcgt                                                                                                                               >  2:1223085/3‑229 (MQ=255)

                                                                                                                tCTGCCTACGATTGCGATTCTGA‑CCGCCGCAGCAAGTCTGGGCTGCTGGCTAAGTTATATTCAGGGGCGCTG‑GTTAGGG‑AATACC‑AAAA‑CGGTGAAAGGCT‑GGCTGGCA‑CAGCTTCCTGCTAAATAT‑CACCAGCG‑CGCCACCTGCA‑TGTTTGACCGCCACGGTCTGCTGGCGCTGCTGGCTGGACGTTTTCTTGCATTTGTCCGTACGCTGCTGCCAACCATGGCGGGAATTTCCgg                                                                                                                               >  1:1195641/1‑237 (MQ=255)

                                                                                                                tCTGCCTACGATTGCGATTCTGA‑CCGCCGCAGCAAGTCTGGGCTGCTGGCTAAGTTATATTCAGGGGCGCTG‑GTTAGGG‑AATACC‑AAAA‑CGGTGAAAGGCT‑GGCTGGCA‑CAGCTTCCTGCTAAATAT‑CACCAGCG‑CGCCACCTGCA‑TGTTTGACCGCCACGGTCTGCTGGCGCTGCTGGCTGGACGTTTTCTTGCATTTGTCCGTACGCTGCTGCCAACCATGGCGGGAATTTCCgg                                                                                                                               >  1:1043507/1‑237 (MQ=255)

                                                                                                                              cGATTCTGA‑CCGCCGCAGCAAGTCTGGGCTGCTGGCTAAGTTATATTCAGGGGCGCTG‑GTTAGGG‑AATACC‑AAAA‑CGGTGAAAGGCT‑GGCTGGCA‑CAGCTTCCTGCTAAATAT‑CACCAGCG‑CGCCACCTGCA‑TGTTTGACCGCCACGGTCTGCTGGCGCTGCTGGCTGGACg                                                                                                                                                                                  <  1:378343/172‑1 (MQ=255)

                                                                                                                              cGATTCTGA‑CCGCCGCAGCAAGTCTGGGCTGCTGGCTAAGTTATATTCAGGGGCGCTG‑GTTAGGG‑AATACC‑AAAA‑CGGTGAAAGGCT‑GGCTGGCA‑CAGCTTCCTGCTAAATAT‑CACCAGCG‑CGCCACCTGCA‑TGTTTGACCGCCACGGTCTGCTGGCGCTGCTGGCTGGACg                                                                                                                                                                                  >  2:378343/1‑172 (MQ=255)

                                                                                                                              cGATTCTGA‑CCGCCGCAGCAAGTCTGGGCTGCTGGCTAAGTTATATTCAGGGGCGCTG‑GTTAGGG‑AATACC‑AAAA‑CGGTGAAAGGCT‑GGCTGGCA‑CAGCTTCCTGCTAAATAT‑CACCAGCG‑CGCCACCTGCA‑TGTTTGACCGCCACGGTCTGCTGGCGCTGCTGGCTGGACGTTTTCTTGCATTTGTCCGTACGCTGCTGCCAACCATGGCGGGAATTTCCGGTCTGCCAAAccgc‑c                                                                                                                >  1:879598/1‑237 (MQ=255)

                                                                                                                                aTTCTGA‑CCGCCGCAGCAAGTCTGGGCTGCTGGCTAAGTTATATTCAGGGGCGCTG‑GTTAGGG‑AATACC‑AAAA‑CGGTGAAAGGCT‑GGCTGGCA‑CAGCTTCCTGCTAAATAT‑CACCAGCG‑CGCCACCTGCA‑TGTTTGACCGCCACGGTCTGCTGGCGCTGCTGGCTGGACGTTTTCTTGCATTTGTCCGTACGCTGCTGCCAACCATGGCGGGGATTTTCGGTCTGCCAAACCGC‑CGCt                                                                                                             >  1:417810/1‑238 (MQ=255)

                                                                                                                                aTTCTGA‑CCGCCGCAGCAAGTCTGGGCTGCTGGCTAAGTTATATTCAGGGGCGCTG‑GTTAGGG‑AATACC‑AAAA‑CGGTGAAAGGCT‑GGCTGGCA‑CAGCTTCCTGCTAAATAT‑CACCAGCG‑CGCCACCTGCA‑TGTTTGACCGCCACGGTCTGCTGGCGCTGCTGGCTGGACGTTTTCTTGCATTTGTCCGTACGCTGCTGCCAACCATGGCGGGAATTTCCGGTCTGCCAAACCGC‑CGCt                                                                                                             >  1:1106260/1‑238 (MQ=255)

                                                                                                                                   tcggcccgccgCAGCAAGTCTGGGCTGTTGGTTAAGTTATATTCAGGGGCGCTG‑GTTAGGG‑AATACC‑AAAA‑CGGTGAAAGGCT‑GGCTGGCA‑CAGCTTCCTGCTAAATAT‑CACCAGCG‑CGCCCCCTGCT‑TGTTTGCCCGCCCCGGTCTGCTGGCGCTGCTGGCTGGACGTTTTCTTGCATTTGTCCGTACGCTGCTGCCAACCATGGCGGGAATTTCCGGTCTGCCAAACCGC‑CGCTTcc                                                                                                          <  2:371189/234‑1 (MQ=255)

                                                                                                                                   tccacccgcctCATCATGTCTGGGCTGCTTGCTAAGTTATATTGGGGGGCGTGT‑TTTAGGA‑AATACC‑AAAC‑CGTTGAAAGGCT‑GCCTGGCA‑CAGCTTCCTGCTAAATAT‑ACCCAGCG‑CGCCCCCTGCA‑TTTTTGCCCGCCACGGTCTGCTGTCGCTGCTGGCTGGACGTTTTCTTGCATTTTTCCGTACGCTGCTGCCAACCATGGCGGGAATTTCCGGTCTCCCAATCCGC‑CGCTTcc                                                                                                          <  2:287904/234‑1 (MQ=255)

                                                                                                                                   cTGA‑CCGCCGCAGCAAGTCTGGGCTGCTGGCTAAGTTATATTCAGGGGCGCTG‑GTTAGGG‑AATACC‑AAAA‑CGGTGAAAGGCT‑GGCTGGCA‑CAGCTTCCTGCTAAATAT‑CACCAGCG‑CGCCACCTGCA‑TGTTTGACCGCCACGGTCTGCTGGCGCTGCTGGCTGGACGTTTTCTTGCATTTGTCCGTACGCTGCTGCCAACCATGGCGGGAATTTCCGGTCTGCCAAACCGC‑CGCTTCCa                                                                                                         >  1:870980/1‑239 (MQ=255)

                                                                                                                                      a‑CCGCCGCAGCAAGTCTGGGCTGCTGGCTAAGTTATATTCAGGGGCGCTG‑GTTAGGG‑AATACC‑AAAA‑CGGTGAAAGGCT‑GGCTGGCA‑CAGCTTCCTGCTAAATAT‑CACCAGCG‑CGCCACCTGCA‑TGTTTGACCGCCACGGTCTGCTGGCGCTGCTGGCTGGACGTTTTCTTGCATTTGTCCGTACGCTGCTGCCAACCATGGCGGGAATTTCCGGTCTGCCAAACCGC‑CGCTTCCCGtt                                                                                                      >  1:1201698/1‑239 (MQ=255)

                                                                                                                                            ggcaacaAGTCGGGGCTGTGGGTTAGTTTATATTCGGGGGCGCGT‑GTTAGGA‑AATACC‑AAAA‑CGGTGAAAGGCT‑GGCTGGCA‑CAGCTTCCTGCAAAATAC‑CCCCAGCG‑CGCCCCCTGCA‑TGTTTGACCGCCACGGTCTGCTGGCGCTGCTGGCTGGACGTTTTCTTGCATTTGTCCGTACGCTGCTGCCAACCATGGCGGGAATTTCCGGTCTGCCAAACCGC‑CGCTTCCAGTTTTTc                                                                                                  <  2:701937/237‑1 (MQ=255)

                                                                                                                                            cgcAGCAAGTCTGGGCTGCTGGCTAAGTTATATTCAGGGGCGCTG‑GTTAGGG‑AATACC‑AAAA‑CGGTGAAAGGCT‑GGCTGGCA‑CAGCTTCCTGCTAAATAT‑CACCAGCG‑CGCCACCTGCA‑TGTTTGACCGCCACGGTCTGCTGGCGCTGCTGGCTGGACGTTTTCTTGCATTTGTCCGTACGCTGCTGCCAACCATGGCGGGAATTTCCGGTCTGCCAAACCGC‑CGCTTCCAGtttttta                                                                                                 >  1:1076133/1‑237 (MQ=255)

                                                                                                                                               agcaAGTCTGGGCTGCTGGCTAAGTTATATTCAGGGGCGCGG‑TTTAGGA‑AATACC‑AAAA‑CGGGGAAAGGCT‑GGCTGGCA‑CATCTTCCTGCTAAATAT‑CCCCAGCG‑CGCCACCTGCA‑TTTTTGACCGCCACGGTCTGCTGGCGCTGCTGGCTGGACGTTTTCTTGCATTTGTCCGTACGCTGCTGCCAACCATGGCGGGAATTTCCGGTCTGCCAAACCGC‑CGCTTCCAGTTTTTCAAc                                                                                               <  1:458093/238‑1 (MQ=255)

                                                                                                                                                           tggttgGCAAAGTTATATTCGGGGGGGCTG‑GTTGGGA‑AATACC‑AAAC‑GGGTAAAGGGTG‑GGCGGGCA‑CAGCTCCCTGTAAAATAC‑CCCCAGCG‑CCCCCCCTGAT‑TGTTTGACCGCCACGGTCGGCTGGCGCTGCTGGCTGGACGTTTTCTTGCATTTGTCCGTACGCTGCTGCAAACCATGGCGGGAATTTCCGGTCTGCCAAACCGC‑CGCTTCCAGTTTTTCAACTGGTTAAGTGGa                                                                                   <  2:879598/234‑1 (MQ=255)

                                                                                                                                                           aaaagagacaggTTATATTCAGGGGCGCTG‑GTTAGGG‑AACACA‑AAAC‑CGGGGAAAGGCT‑GGCTGGCA‑CAGTTTCCTGCTAAATAC‑CCCCGGCG‑CGCCCCCTGCA‑TGTTTGACCGCCCCGGTCTGCTGGCGCTGCTGGCTGGACGTTTTCTTGCATTTGTCCGTACGCTGCTGCCAACCATGGCGGGAATTTCCGGTCTGCCAAACCGC‑CGCTTCCAGTTTTTCAACTGGTTAAGTGGAt                                                                                  <  2:1098724/228‑1 (MQ=255)

                                                                                                                                                            tgctgGTAAAGTTATATTGAGGGGCGCTG‑GTTAGGA‑AAAACA‑AAAA‑CGGTGAAAGCCG‑GGCTGGCA‑CAGCTTCCTGCAAAATAT‑CCCCTGCG‑CGCCCCCTGCA‑TTTTGGCCCCCCCCGGTCTGCGGGCGCTGCTGGCTGGACGTTTTCTTGCATTTGTCCGTACGCTGCTGCCAACCATGGCGGGAATTTCGGGTCTGCCAAACCGC‑CGCTTCCAGTTTTTCAACTGGTTAAGTGGAt                                                                                  <  2:1043507/238‑1 (MQ=255)

                                                                                                                                                            tgctgGCTAAGTTATATTCAGGGGCGCTG‑TTTAGGG‑AATACC‑AAAA‑CGGTGAAAGGCT‑GGCTGGCA‑CAGCTTCCTGCTAAATAT‑CACCAGCG‑CGCCACCTGCA‑TGTTTGACCGCCACGGTCTGCTGGCGCTGCTGGCTGGACGTTTTCTTGCATTTGTCCGTACGCTGCTGCCAACCATGGCGGGAATTTCCGGTCTGCCAAACCGC‑CGCTTCCAGTTTTTCAACTGGTTAAGTGGAt                                                                                  <  1:1071573/238‑1 (MQ=255)

                                                                                                                                                            tgctgGCTAAGTTATATGCAGGGGCGCTG‑TTTAGGA‑AATACC‑AAAA‑CGGTGAAAGGCT‑GGCTGGCA‑CGGCTTCCTCCAAAATAC‑CACCAGGG‑CGCCACCTGCA‑TGTTTGACCGCCACGGTCTGCTGGCGCTGCTGGCTGGACGTTTTCTTGCATTTGTCCGTACGCTGCTGCCAACCATGGCGGGAATTTCCGGTCTGCCAAACCGC‑CGCTTCCAGTTTTTCAACTGGTTAAGTGGAt                                                                                  <  1:234769/238‑1 (MQ=255)

                                                                                                                                                            tgctgGCTAAGTTATATCCAGGGGCGCTG‑GTTAGGG‑AATCCC‑AAAC‑CGGTGAAAGGCT‑GGCTGGCA‑CAGCTTCCTGCTAAATAT‑CACCAGCG‑CGCCCCCTGCA‑TGTTTGACCGCCACGGTCTGCTGGCGCTGCTGGCTGGACGTTTTCTTGCATTTGTCCGTACGCTGCTGCCAACCATGGCGGGAATTTCCGGTCTGCCAAACCGC‑CGCTTCCAGTTTTTCAACTGGTTAAGTGGAt                                                                                  <  1:826956/238‑1 (MQ=255)

                                                                                                                                                            tgcgggtaaatttATTTTCGGGGGCTCGG‑TTTAGGA‑AATCCA‑AAAA‑GGGTGAAAGGCT‑GGCGGGCC‑CAGCTCCCTGCTAAATAT‑CACCGGCG‑CGCCCCCTGCT‑TTTTTGCCCGCCACGGTCTGCTGGCGCTGCTGGCTGGACGTTTTCTTGCATTTGTCCGTACGCTGCTGCCAACCATGGCGGGAATTTCCGGTCTGCCAAACCGC‑CGCTTCCAGTTTTTCAACTGGTTAAGTGGAt                                                                                  <  2:37581/227‑1 (MQ=255)

                                                                                                                                                            tgcggctaaaGTTATTTCCGGGGGCCCGG‑TTTAGGA‑AATCCC‑AAAC‑CGG‑GAAAGGGTGGGCTGGCA‑CCGTTCCTTGCAAAATAC‑CCCCGGCG‑CGCCCCCTGCT‑TTTTTGCCCGCCACGGTCTGCTGGCGCTGCTGGCTGGACGTTTTCTTGCATTTGTCCGTACGCTGCTGCCAACCATGGCGGGAATTTCCGGTCTGCCAAACCGC‑CGCTTCCAGTTTTTCAACTGGTTAAGTGGAt                                                                                  <  2:544883/230‑1 (MQ=255)

                                                                                                                                                              ctgGCTAAGTTATATTCAGGGGCGCTG‑GTTAGGG‑AATACC‑AAAA‑CGGTGAAAGGCT‑GGCTGGCA‑CAGCTTCCTGCTAAATAT‑CACCAGCG‑CGCCACCTGCA‑TGTTTGACCGCCACGGTCTGCTGGCGCTGCTGGCTGGACGTTTTCTTGCATTTGTCCGTACGCTGCTGCCAACCATGGCGGGAATTTCCGGTTTGCCAAACCGC‑CGCTTCCAGTTTTTCAACTTGTTAAggggtat                                                                                 >  2:1084955/1‑234 (MQ=255)

                                                                                                                                                                     aGTTATATTCAGGGGCGCTG‑GTTAGGG‑AATACC‑AAAA‑CGGTGAAAGGCT‑GGCTGGCA‑CAGCTTCCTGCTAAATAT‑CCCCAGCG‑CGCCACCTGCA‑TGTTTGACCGCCACGGTCTGCTGGCGCTGCTGGCTGGACGTTTTCTTGCATTTGTCCGTACGCTGCTGCCAACCATGGCGGGAATTTCCGGTCTGCCAAACCGC‑CGCTTCCAGTTTTTCAACTGGTTAAGTGGATTGCTGTGGGt                                                                        <  1:915965/239‑1 (MQ=255)

                                                                                                                                                                     aGTAATATTGAGGGGCGCTG‑GTTA‑GG‑AAAACC‑AAAAGCGGTGAAAGGCG‑GGTGGGCG‑CAGCTTCCTGCTAAATAC‑CCCCAGCG‑CGCCACCTGCA‑TGTTGGCCCGCCACGGTCTGCTGGCGCTGCTGGCTGGACGTTTTCTTGCATTTGTCCGTACGCTGCTGCCAACCATGGCGGGAATTTCCGGTCTGCCAAACCGC‑CGCTTCCAGTTTTTCAACTGGTTAAGTGGATTGCTGTGGGt                                                                        <  2:859427/239‑1 (MQ=255)

                                                                                                                                                                         ttatttcggggggCTGTGTTGGGGAAATACA‑AAAA‑GGGTGAAGGGTT‑GGCTGGCA‑CAGTTTCCTGCTAAATAC‑CCCCGGGG‑CGCCCCCTGCA‑TGTTTGCCCGCCACGGTCTGCTGGCGCTGCTGGCTGGACGTTTTCTTGCATTTGTCCGTACGCTGCTGCCAACCATGGCGGGAATTTCCGGTCTGCCAAACCGC‑CGCTTCCAGTTTTTCAACTGGTTAAGTGGATTGCTGTGGGTCa                                                                      <  2:325276/232‑1 (MQ=255)

                                                                                                                                                                           atTCAGGGGCGCTG‑GTTAGGG‑AATACC‑AAAA‑CGGTGAAAGGCT‑GGCTGGCA‑CAGCTTCCTGCTAAATAT‑CACCAGCG‑CGCCACCTGCA‑TGTTTGACCGCCACGGTCTGCTGGCGCTGCTGGCTGGACGTTTTCTTGCATTTGTCCGTACGCTGCTGCCAACCATGGCGGGAATTTCCGGTCTGCCAAACCGC‑CGCTTCCAGTTTTTCAACTGGTTAAGTGGATTGCTGTGGGTCAGCGt                                                                  >  1:859427/1‑239 (MQ=255)

                                                                                                                                                                           atTCAGGGGCGCTG‑GTTAGGG‑AATACC‑AAAA‑CGGTGAAAGGCT‑GGCTGGCA‑CAGCTTCCTGCTAAATAT‑CACCAGCG‑CGCCACCTGCA‑TGTTTGACCGCCACGGTCTGCTGGCGCTGCTGGCTGGACGTTTTCTTGCATTTGTCCGTACGCTGCTGCCAACCATGGCGGGAATTTCCGGTCTGCCAAACCGC‑CGCCTCCAGTTTTTCAACTGGTTTAGTGGATTGCTGGGGGTCAGCGt                                                                  >  1:593777/1‑239 (MQ=255)

                                                                                                                                                                                ggggCGCTG‑GTTAGGG‑AATACC‑AAAA‑CGGTGAAAGGCT‑GGCTGGCA‑CAGCTTCCTGCTAAATAT‑CACCAGCG‑CGCCACCTGCA‑TGTTTGACCGCCACGGTCTGCTGGCGCTGCTGGCTGGACGTTTTCTTGCATTTGTCCGTACGCTGCTGCCAACCATGGCGGGAATTTCCGGTCTGCCAAACCGC‑CGCTTCCAGTTTTTCAACTGGTTAAGTGGATTGCTGTGGGTCAGCGTGGTa                                                              >  1:221001/1‑238 (MQ=255)

                                                                                                                                                                                 gggCGCTG‑GTTAGGG‑TATACC‑AAAA‑CGGTGAAAGGCT‑GGCTGGCA‑CAGCTTCCTGCTAAATAT‑CACCAGCG‑CGCCACCTGCA‑TGTTTGACCGCCACGGTCTGCTGGCGCTGCTGGCTGGACGTTTTCTTGCATTTGTCCGTACGCTGCTGCCAACCATGGCGGGAATTTCCGGTCTTCCAAACCGC‑CGCTTCCAGTTTTTCCCCTGGTTAAGTGGATTGCTGTGGGTCCGCGTGGTaa                                                             >  1:139883/1‑238 (MQ=255)

                                                                                                                                                                                 gggCGCTG‑GTTAGGG‑AATACC‑AAAA‑CGGTGAAAGGCT‑GGCTGGCA‑CAGCTTCCTGCTAAATAT‑CACCAGCG‑CGCCACCTGCA‑TGTTTGACCGCCACGGTCTGCTGGCGCTGCTGGCTGGACGTTTTCTTGCATTTGTCCGTACGCTGCTGCCAACCATGGCGGGCATTTTCGGTCTGCCAAACCGC‑CGCTTCCAGTTTTTCAACTGGTTAAGTGGATTGCTTCGGGTCAGCGTGGGaa                                                             >  2:1200807/1‑238 (MQ=255)

                                                                                                                                                                                 gggCGCTG‑GTTAGGG‑AATACC‑AAAA‑CGGTGAAAGGCT‑GGCTGGCA‑CAGCTTCCTGCTAAATAT‑CACCAGCG‑CGCCACCTGCA‑TGTTTGACCGCCACGGTCTGCTGGCGCTGCTGGCTGGACGTTTTCTTGCATTTGTCCGTACGCTGCTGCCAACCATGGCGGGAATTTCCGGTCTGCCCAACCGC‑CCCTTCCCGTTTTTCAACTGGTTTAGTGGATTGCTGTGGGTCCGCGTGGGaa                                                             >  2:863741/1‑238 (MQ=255)

                                                                                                                                                                                 gggCGCTG‑GTTAGGG‑AATACC‑AAAA‑CGGTGAAAGGCT‑GGCTGGCA‑CAGCTTCCTGCTAAATAT‑CACCAGCG‑CGCCACCTGCA‑TGTTTGACCGCCACGGTCTGCTGGCGCTGCTGGCTGGACGTTTTCTTGCATTTGTCCGTACGCTGCTGCCAACCATGGCGGGAATTTCCGGTCTGCCAAACCGC‑CGCTTCCCGTTTTTCAACTGGTTAAGTGGATTGCTGTGGGt                                                                        >  1:1098724/1‑227 (MQ=255)

                                                                                                                                                                                 gggCGCTG‑GTTAGGG‑AATACC‑AAAA‑CGGTGAAAGGCT‑GGCTGGCA‑CAGCTTCCTGCTAAATAT‑CACCAGCG‑CGCCACCTGCA‑TGTTTGACCGCCACGGTCTGCTGGCGCTGCTGGCTGGACGTTTTCTTGCATTTGTCCGTACGCTGCTGCCAACCATGGCGGGAATTTCCGGTCTGCCAAACCGC‑CGCTTCCAGTTTTTCAACTGGTTAAGTGGATTGCTGTGGGTCAGCGTGGTaa                                                             >  1:249410/1‑238 (MQ=255)

                                                                                                                                                                                  ggggcTG‑GTTAGGA‑AATCCC‑AAAC‑GGGTGAAAGGCT‑GGCGGCCA‑CAGTTCCCTGCAAAATAC‑CCCCGGCG‑CCCCCCCTGCA‑TGTTTGACCGCCACGGTCTGCTGGCGCTGCTGGCTGGACGTTTTCTTGCATTTGTCCGTACGCTGCTGCCAACCATGGCGGGAATTTCCGGTCTGCCAAACCGC‑CGCTTCCAGTTTTTCAACTGGTTAAGTGGATTGCTGTGGGTCAGCGTGGTAAc                                                            <  2:1139761/235‑1 (MQ=255)

                                                                                                                                                                                    gggaggtgtAGGG‑AAAACCAAAAA‑CGGTAAAAGGCG‑GGCGGGCA‑CAGCTTCCGGCAAAATAC‑CCCCAGCG‑CGCCCCCTGCT‑GTTTTGCCCGCCACGGTCTGCTGGCGTTGCTGGCTGGACGTTTTCTTGCATTTGTCCGTCCGCTGCTGCCAACCATGGCGGGAATTTCCGGTCTGCCAAACCGC‑CGCTTCCAGTTTTTCAACTGGTTAAGTGGATTGCTGTGGGTCAGCGTGGTAAc                                                            <  2:730365/230‑1 (MQ=255)

                                                                                                                                                                                    cgcTG‑GTTAGGG‑AATACC‑AAAA‑CGGTGAAAGGCT‑GGCTGGCA‑CAGCTTCCTGCTAAATAT‑CACCAGCG‑CGCCACCTGCA‑TGTTTGACCGCCACGGTCTGCTGGCGCTGCTGGCTGGACGTTTTCTTGCATTTGTCCGTACGCTGCTGCCAACCATGGCGGGAATTTCCGGTCTGCCAAACCGC‑CGCTTCCAGTTTTTCAACTGGTTAAGTGGATTGCTGTGGGTCAGCGTGGTAACCAg                                                         >  1:836717/1‑239 (MQ=255)

                                                                                                                                                                                              gggaagtACA‑AAAC‑CGGTGAAAGCCT‑‑GCTGGCACCATCTTCCGCTTAATTAT‑CACCAGCG‑CGCCCCCTCCT‑TTTTTGCGCGCCACGTTCTTCTGGCGCGGCTGGCTGGACGTTTTCTGGCAGTTGTCCAGACGCTGCTCCCACCCATGGCGGGATTTTCCGGTCTGCCAAGCCGC‑CGCTTCCAGTTTTTCAACTGGTTAAGTGGATTTCTGTGGGTCAGCGTGGTAACCAGTTTTGGCt                                                 <  2:374625/233‑1 (MQ=255)

                                                                                                                                                                                               ggaaaTCCC‑AAAA‑CGGGGAAGGGCG‑GGGTGACA‑CAGTTTCTTGTTAAATAT‑CACCAGGG‑CGCCCCCTGTT‑TGTTTGCCCGCCACGGTCTGCTGGCGCTGCTGGCTGGACGTTTTCTTGCATTTGTCCGTACGCTGCTGCCAACCATGGCGGGAATTTCCGGTCTGCCAAACCGC‑CGCTTCCAGTTTTTCAACTGGTTAAGTGGATTGCTGTGGGTCAGCGTGGTAACCAGTTTTGGCt                                                 <  2:1024023/235‑1 (MQ=255)

                                                                                                                                                                                               gg‑AATCCA‑AAAA‑CGGGAAAAGGCT‑GGCTGGCC‑CAGCTTCCTGTTAAATAT‑CCCCGGCG‑CGCCACCTGCA‑TGTTTGACCGCCTCGGTCTGCTGCCGCTGCTGGCTGGACGTTTTCTTGCATTTGTCCGTACGCTGCTGCCAACCATGGCGGGAATTTCCGGTCTGCCAAACCGC‑CGCTTCCAGTTTTTCAACTGGTTAAGTGGATTGCTGTGGGTCAGCGTGGTAACCAGTTTTGGCTa                                                <  1:1131947/238‑1 (MQ=255)

                                                                                                                                                                                                gaaaTCCC‑AAAA‑CGGTGAAAGGCG‑GGCGGGCC‑CAGTTTCCTGTTAAATTA‑CCCCAGCG‑CGCCACCTGCA‑TGTTTGCCCGCCACGGTCTGCTGGCGCTGCTGGCTGGACGTTTTCTTGCATTTGTCCGTACGCTGCTGCCAACCATGGCGGGAATTTCCGGTCTGCCAAACCGC‑CGCTTCCAGTTTTTCAACTGGTTAAGTGGATTGCTGTGGGTCAGCGTGGTAACCAGTTTTGGCTa                                                <  2:584861/236‑1 (MQ=255)

                                                                                                                                                                                                   aTACC‑AAAA‑CGGTGAAAGGCT‑GGCTGGCA‑CAGCTTCCGCCTAAATAT‑CCCCAGCG‑CGCCACCTGCA‑GGTTTTCCCGCCACGGTCTGCTGGCGCTGCTGGCTGGCCGTTTTCTTGCATTTGTCCGTACGCTGCTGCCAACCATGGCGGGAATTTCCGGTCTGCCAAACCGC‑CGCTTCCAGTTTTTCAACTGGTTAAGTGGATTGCTGTGGGTCAGCGTGGTAACCAGTTTTGGCTATGcc                                            <  1:211314/239‑1 (MQ=255)

                                                                                                                                                                                                     aCC‑AAAC‑CGGTGAAGGGCG‑GTCTGGCC‑CTG‑TTTCTCCTAAATTTCCCCCGGCC‑CCCCCCCTGCT‑TTTTTGCCCGCCCCGTTCGCCTGGCGCTGCTGGCTGGACGTTTTCTTGCATTTGTCCGTACGCTGCTGCCAACCATGGCGGGAATTTCCGGTCTGCCAAACCGC‑CGCTTCCAGTTTTTCAACTGGTTAAGTGGATTGCTGTGGGTCAGCGTGGTAACCAGTTTTGGCTATGCCtt                                          <  2:907634/239‑1 (MQ=255)

                                                                                                                                                                                                     aCC‑AAAA‑CGGTGAAAGGCT‑GGCTGGCA‑CAGCTTCCTGCTAAATAT‑CACCAGCG‑CGCCACCTGCA‑TGTTTGACCGCCACg                                                                                                                                                                                                           <  1:603906/79‑1 (MQ=255)

                                                                                                                                                                                                     aCC‑AAAA‑CGGTGAAAGGCT‑GGCTGGCA‑CAGCTTCCTGCTAAATAT‑CACCAGCG‑CGCCACCTGCA‑TGTTTGACCGCCACGGTCTGCTGGCGCTGCTGGCTGGACGTTTTCTTGCATTTTTCCGTACGCTGCTGCCAACCATGGCGGGAATTTTCCTTCTCCCAAACCGC‑CGCTTCCCGCTTTTCAACTGGTTAAGTGGATTGCTGTGGGTCAGCGTGGTAACCAGTTTTGGCTATGCCtt                                          >  2:265164/1‑239 (MQ=255)

                                                                                                                                                                                                     aCC‑AAAA‑CGGTGAAAGGCT‑GGCTGGCA‑CAGCTTCCTGCTAAATAT‑CACCAGCG‑CGCCACCTGCA‑TGTTTGACCGCCACGGTCTGCTGGCGCTGCTGGCTGGACGTTTTCTTGCATTTGTCCGTACGCTGCTGCCAACCATGGCGGGAATTTCCGGTCTGCCAAACCGC‑CGCTTCCCGGTTTTTCACTGGTTAAGTGGGTTGCTGGGGGGCAGCGGGGTGACCAGGTTTGGCTTTGCCtt                                          >  2:21944/1‑239 (MQ=255)

                                                                                                                                                                                                      actaaaa‑CGGTGAAAGGCT‑GGCTGGCA‑CAGCTTCCTGCTAAATAT‑CACCAGCG‑CGCCACCTGCA‑TGTTTGACCGCCACGGTCTGCTGGCGCTGCTGGCTGGACGCTTTCTTGCATTTGTCCGTACGCTGCTGCCAACCATGGCGGGAATTTCCGGTCTGCCAAACCGC‑CGCTTTCCGCTTTTCATCTGGTTAAGGGGTTTGCTGTAGGTCAGCGGGGAAACGAGTTTTTGCTATGccct                                          >  2:105811/4‑237 (MQ=255)

                                                                                                                                                                                                           aa‑CGGTGAAAGGCT‑GGCTGGCA‑CAGCTTCCTGCTAAATAT‑CACCAGCG‑CGCCACCTGCA‑TGTTTGACCGCCACGGTCTGCTGGCGCTGCTGGCTGGACGTTTTCTTGCATTTGTCCGTACGCTGCTGCCAACCATGGCGGGAATTTCCGGTCTGCCAAACCGC‑CGCTTCCAGTTTTTCAACTGGTTAAGTGGATTGCTGTGGGTCCGCGTGGTAACCAGTTTTGGCCTGGCCTTAAGtg                                     >  2:1264833/1‑238 (MQ=255)

                                                                                                                                                                                                           aa‑CGGTGAAAGGCT‑GGCTGGCA‑CAGCTTCCTGCTAAATAT‑CACCAGCG‑AGCCACCTGCA‑TGTTTGACCGCCACGGTCTGCTGGCGCTGCTGGCTGGACGTTTTCTTGCATTTGTCCGTACGCTGCTGCCAACCATGGCGGGTATTTCCGGTCTTCCAAACCGCGCGTTTCC‑GCTTTTCAACTGGCTAGGCGGCTTGGTGTGGGTCCGCGTGGTAACCAGATTTGGCGATGGCCTTAGTa                                     >  2:1299587/1‑239 (MQ=255)

                                                                                                                                                                                                               ggTGAAAGGCT‑GGCTGGCA‑CAGCTTCCTGCTAAATAT‑CACCAGCG‑CGCCACCTGCA‑TGTTTGACCGCCACGGTCTGCTGGCGCTGCTGGCTGGACGTTTTCTTGCATTTGTCCGTACGCTGCTGCCAACCATGGCGGGAATTTCCGGTCTGCCAAACCGC‑CGCTTCCAGTTTTTCAACTGGTTAAGTGGATTGCTGTGGGTCAGCGTGGTAACCAGTTTTGGCTATGCCTTAAGTATGa                                  <  1:1359118/239‑1 (MQ=255)

                                                                                                                                                                                                                 tGAAAGGCT‑GGCTGGCA‑CAGCTTCCTGCTAAATAT‑CACCAGCG‑CGCCACCTGCA‑TGTTTGACCGCCACGGTCTGCTGGCGCTGCTGGCTGGACGTTTTCTTGCATTTGTCCGTACGCTGCTGCCAACCATGGCGGGAATTTCCGGTCTGCCAAACCGC‑CGCTTCCAGTTTTTCAACTGGTTAAGTGGATTGCTGTGGGTCAGCGTGGTAACCAGTTTTGGCTATGCCTTAAGTATGa                                  >  1:1035802/1‑237 (MQ=255)

                                                                                                                                                                                                                 tGAAAGGCT‑GGCTGGCA‑CAGCTTCCTGCTAAATAT‑CACCAGCG‑CGCCACCTGCA‑TGTTTGACCGCCACGGTCTGCTGGCGCTGCTGGCTGGACGTTTTCTTGCATTTGTCCGTACGCTGCTGCCAACCATGGCGGGAATTTCCGGTCTGCCAAACCGC‑CGCTTCCAGTTTTTCAACTGGTTAAGTGGATTGCTGTGGGGCAGGGTGGGAACCAGTTTTGGCTATGCCTTaaagatgtt                                 >  2:917415/1‑231 (MQ=255)

                                                                                                                                                                                                                 tGAAAGGCT‑GGCTGGCA‑CAGCTTCCTGCTAAATAT‑CACCAGCG‑CGCCACCTGCA‑TGTTTGACCGCCACGGTCTGCTGGCGCTGCTGGCTGGACGTTTTCTTGCATTTGTCCGTACGCTGCTGCCAACCATGGCGGGAATTTCCGGTCTGCCAAACCGC‑CGCCTCCAGTTTTTCCACTGGGTAAGTGGGTTGCTGTGGGCTCGCGGGGTACCCAGGTTTGGGTATGCCCTAAGtcttgt                                 >  2:1018781/1‑233 (MQ=255)

                                                                                                                                                                                                                 tGAAAGGCT‑GGCTGGCA‑CAGCTTCCTGCTAAATAT‑CACCAGCG‑CGCCACCTGCA‑TGTTTGACCGCCACGGTCTGCTGGCGCTGCTGGCTGGACGTTTTCTTGCATTTGTCCGTACGCTGCTGCCAACCATGGCGGGAATTTCCCGTCTGCCAAACCGC‑CGCTTCCCGGTTTTCAACTGGTTAAGTGGTTTGCTTTGGGTCAGCGTGGTAACCAGTTTTGGCTATGCCTTAAGTATGAt                                 >  2:155729/1‑238 (MQ=255)

                                                                                                                                                                                                                   aaaGGCT‑GGCTGGCA‑CAGCTTCCTGCTAAATAT‑CACCAGCG‑CGCCACCTGCA‑TGTTTGACCGCCACGGTCTGCTGGCGCTGCTGGCTGGACGTTTTCTTGCATTTGTCCGTACGCTGCTGCCAACCATGGCGGGAATTTCCGGTCTGCCAAACCGC‑CGCTTCCAGTTTTTCAACTGGTTAAGTGGGTTGCTGTGGGTCAGCGTGGTAACCAGTTTTGGCTATGCCTTAAGTATGATTcc                              >  1:1029917/1‑239 (MQ=255)

                                                                                                                                                                                                                       gct‑ggctgCCC‑CACCTCCCTGTAAAATAC‑CCCCGGCG‑CGCCCCTTGCA‑GGTTTGCCCGCCCCGGTCTGCTGGCGCTGCTGGCTGGACGTTTTCTTGCATTTGTCCGTACGCTGCTGCCAACCATGGCGGAAATTTCCGGTCTGCCAAACCGC‑CGCTTCCAGTTTTTCAACTGGTTAAGTGGATTGCTGTGGGTCAGCGTGGTAACCAGTTTTGGCTATGCCTTAAGTATGATTCcgt                            <  2:1183569/237‑1 (MQ=255)

                                                                                                                                                                                                                           ggctggcA‑CAGCTTCCTGCTAAATAT‑CACCAGCG‑CGCCACCTGCA‑TGTTTGACCGCCACGGTCTGCTGGCGCTGCTGGCTGGACGTTTTCTTGCATTTGTCCGTACGCTGCTGCCAACCATGGCGGGGATTTCCGGTCTGCCAAACCGC‑CGCTTCCAGCTTTTCAACTGGGTAAGTGGATTGCTGTGGGTCCGCGTGGTGACCAGTTTTGGCTCTGCCTTAAGTATGATTCcgttcgtt                       >  2:317854/1‑239 (MQ=255)

                                                                                                                                                                                                                            gctggcA‑CAGCTTCCTGCTAAATAT‑CACCAGCG‑CGCCACCTGCA‑TGTTTGACCGCCACGGTCTGCTGGCGCTGCTGGCTGGACGTTTTCTTGCATTTGTCCGTACGCTGCTGCCAACCATGGCGGGGATTTCCCGTCTGCCAAACCGC‑CGCTTCCAGTTTTTCAACTGGTTTAGTGGTTTGCTGTGGGTCAGCGTGGCAACCAGCTTTGGCTCTGCCTTCAGAAAGATTCCGTTCGTTa                      >  2:1027797/1‑239 (MQ=255)

                                                                                                                                                                                                                            gctggcA‑CAGCTTCCTGCTAAATAT‑CACCAGCG‑CGCCACCTGCA‑TGTTTGACCGCCACGGTCTGCTGGCGCTGCTGGCTGGACGTTTTCTTGCATTTGTCCGTACGCTGCTGCCAACCATGGCGGGAATTTCCGGTCTGCCAAACCGC‑CGCTTCCAGTTTTTCAACTGGTTAAGTGGATTGCTGGGGGTCCGCGTGGGAACCAGTTTTTGCTATGCCTTTAGTATGATTTCGTTCGGTa                      >  1:691315/1‑239 (MQ=255)

                                                                                                                                                                                                                            gctggcA‑CAGCTTCCTGCTAAATAT‑CACCAGCG‑CGCCACCTGCA‑TGTTTGACCGCCACGGTCTGCTGGCGCTGCTGGCTGGACGTTTTCTTGCATTTGTCCGTACGCTGCTGCCAACCATGGCGGGAATTTCCGGTCTGCCAAACCGC‑CGCTTCCAGTTTTTCAACTGGTTAAGTGGATTGCTGGGGGTCAGCGTGGTAACCAGTTTTGGCTATGCCTTAAGTATGATTCCGTTCGTTa                      >  2:764051/1‑239 (MQ=255)

                                                                                                                                                                                                                                 ca‑caTCTTCCTGCAAAATAC‑CCCCAGCG‑CGCCCCCTGCA‑TGTTTGCCCGCCCGGGTCTGCTGGCTCTGCTGGCTGGACGTTTTCTTGCATTTGTCCGTACGCTGCTGCCAACCAGGGCGGGAATTTCCGGTCTGCAAAACCGC‑CGCTTCCAGTTTTTCAACTGGTTAAGTGGATTGCTGTGGGTCAGCGTGGTAACCAGTTTTGGCTATGCCTTAAGTATGATTCCGTTCGTTAAACg                  <  2:1277181/238‑1 (MQ=255)

                                                                                                                                                                                                                                      gCTTCCTGCTAAAAAC‑CCCCGGCG‑CGCCACCTGAA‑TGTTTGACCGCCACGGTCTGCTGGCGCTGCTGGCTGGACGTTTTCTTGCATTTGTCCGTACGCTGCTGCCAACCATGGCGGGAATTTCCGGTCTGCCAAACCGC‑CGCTTCCAGTTTTTCAACTGGTTAAGTGGATTGCTGTGGGTCAGCGTGGTAACCAGTTTTGGCTATGCCTTAAGTATGATTCCGTTCGTTAAACGCCATg             <  1:357979/239‑1 (MQ=255)

                                                                                                                                                                                                                                        gacgggcaaaaTAT‑CCCCGGCG‑CGCCACGTGCA‑TGTTTGCCCGCCCCGGTCTGCTGGCGCGGCTGGCTGGACGTTTTCTTGCATTTGTCCGTACGCTGCTGCCAACCATGGCGGGAATTTCCGGTCTGCCAAACCGC‑CGCTTCCAGTTTTTCAACTGGTTAAGTGGATTGCTGTGGGTCAGCGTGGTAACCAGTTTTGGCTATGCCTTAAGTATGATTCCGTTCGTTAAACGCCATg             <  1:773565/229‑1 (MQ=255)

                                                                                                                                                                                                                                        cttctggcaaaTTT‑CCCCCGCGCCGCCCCCTGCA‑TGTTGGCCCGCCACGGTCTGCTGGCGCTGCTGGCTGGACGTTTTCTTGCATTTGTCCGTACGCTGCTGCCAACCATGGCGGGAATTTCCGGTCTGCCAAACCGC‑CGCTTCCAGTTTTTCAACTGGTTAAGTGGATTGCTGTGGGTCAGCGTGGTAACCAGTTTTGGCTATGCCTTAAGTATGATTCCGTTCGTTAAACGCCa               <  1:21944/228‑1 (MQ=255)

                                                                                                                                                                                                                                          cgtGCAAAATAC‑CACC‑GCG‑CGCCCGCTGGATTTTTTGCCCGCCCCGGTCTGCTGGCGCTGCGGGCTGGACGTTTCCTTGCTTTTGTCCGTACGCTGCGGCCAACCATGGCGGGAATTTCCGGTCTGCCAAACCGC‑CGCTTCCAGTTTTTCAACTGGTTAAGTGGATTGCTGTGGGTCAGCGTGGTAACCAGTTTTGGCTATGCCTTAAGTATGATTCCGTTCGTTAAACGCCATGAAGa         <  2:593777/237‑1 (MQ=255)

                                                                                                                                                                                                                                           ttGCTAAATAT‑CCCCGGCG‑CGCCACTGGCA‑TGTTTGCCCGCCCCGGTTTGCTGGCGCTGCTGGCTGGACGTTTTCTTGCATTTGGCCGTACGCTGCTGCCAACCATGGCGGGAATTTCCGGTCTGCCAAACCGC‑CGCTTCCAGTTTTTCAACTGGTTAAGTGGATTGCTGTGGGTCAGCGTGGTAACCAGTTTTGGCTATGCCTTAAGTATGATTCCGTTCGTTAAACGCCATGAAGAt        <  1:938647/238‑1 (MQ=255)

                                                                                                                                                                                                                                            tgttAAATAC‑CCCCGGCG‑CGCCCCCTGCT‑TTTTTGCCCGCCACGGTCTGCTGGCGCTGCTGGCTGGACGTTTTCTTGCATTTGTCCGTACGCTGCTGCCAACCATGGCGGGAATTTCCGGTCTGCCAAACCGC‑CGCTTCCAGTTTTTCAACTGGTTAAGTGGATTGCTGTGGGTCAGCGTGGTAACCAGTTTTGGCTATGCCTTAAGTATGATTCCGTTCGTTAAACGCCATGAAGAt        <  2:385048/235‑1 (MQ=255)

CATGTTTGCCACGCTGTTTTTAG‑AAAACGGCCTGCTGCCCGCCTCATTTTTGCCAGGCGACAGCTTGTTGATACTGGCAGGCGCATTGATTGCCCAGGGGGTTATGGATTTTCTGCCTACGATTGCGATTCTGA‑CCGCCGCAGCAAGTCTGGGCTGCTGGCTAAGTTATATTCAGGGGCGCTG‑GTTAGGG‑AATACC‑AAAA‑CGGTGAAAGGCT‑GGCTGGCA‑CAGCTTCCTGCTAAATAT‑CACCAGCG‑CGCCACCTGCA‑TGTTTGACCGCCACGGTCTGCTGGCGCTGCTGGCTGGACGTTTTCTTGCATTTGTCCGTACGCTGCTGCCAACCATGGCGGGAATTTCCGGTCTGCCAAACCGC‑CGCTTCCAGTTTTTCAACTGGTTAAGTGGATTGCTGTGGGTCAGCGTGGTAACCAGTTTTGGCTATGCCTTAAGTATGATTCCGTTCGTTAAACGCCATGAAGATCAGGTA  >  NC_000913/3153655‑3154126

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`