BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	353,673	A→G	12.2%	E323G (GAG→GGG)	prpE →	propionate‑‑CoA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	353,673	0	A	G	12.2%	260.7 / 2.3	110	E323G (GAG→GGG)	prpE	propionate‑‑CoA ligase
Reads supporting (aligned to +/- strand): ref base A (46/48); new base G (14/2); total (60/50)
Fisher's exact test for biased strand distribution p-value = 5.44e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 116 total aligned reads displayed.

GACCGGCAAACCTAAAGGCGTGCAGCGTGACGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCAGCGTGTTCTTTTGCGCATCGGATATCGGCTGGGTGGTGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTATCGTTTACGAAGGATTGCCGACCTGGCC‑GGACTGCGG‑CGT‑GTGGTGGACAATCGTCGAGAAATA‑TCAGG‑TTAGCCGGATGTTCTCA‑GCGCCG‑ACCGCCA‑TTCGCGTGCTGAAAAAATTCCCTACCGCTGAAATTCGCAAACACGATCTCTCGTCGCTGGAAGTGCTCTATCTGGCTGGAGAACCGCTGGACGAGCCGACCGCCAGTTGGGTGAGCAATACGCTGGATGTGCCGGTCATCGACAACTACTGGCAGACCGAATCCGGCTGGCCGATTATGGCGATTGCTCGCGG  >  NC_000913/353434‑353907

gACCGGCAAACCTAAAGGCGTGCAGCGTGACGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCAGCGTGTTC‑TTTGCGCATCGGATATCGGCTGGGTGGTGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTATCGTTTACGAAGGATTGCCGACCTGGCC‑GGACTGCGG‑CGT‑GTGGTGGACAATCGTCga                                                                                                                                                                                                                                                 <  1:900224/239‑1 (MQ=255)

  ccGGCAACCCTAAAGGCGTGCAGGGTGACGTCGGCGGATATGCGGTGGCGCTGGCGTCCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCAGCGTGTTCTTTTGCGCATCGGATATCGGCTGGGTGGTGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTATCGTTTACGAAGGATTGCCGACCTGGCC‑GGACTGCGG‑CGT‑GTGGTGGACAATCGTCga                                                                                                                                                                                                                                                 <  2:1341461/238‑1 (MQ=255)

  ccGGCAAACCTAAAGGCGTGCAGCGTGACGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCAGCGTGTTCTTTTGCGCATCGGATATCGGCTGGGTGGTGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTATCGTTTACGAAGGATTGCCGACCTGGCC‑GGACTGCGG‑CGT‑GTGGTGGACAATCGTCgag                                                                                                                                                                                                                                                <  2:141750/239‑1 (MQ=255)

  ccGGCAAACCTAAAGGCGTGCAGCGTGACGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCAGCGTGTTCTTTTGCGCATCGGATATCGGCTGGGTGGTGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTATCGTTTACGAAGGATTGCCGACCTGGCC‑GGACTGCGG‑CGT‑GTGGTGGACAATCGTCgag                                                                                                                                                                                                                                                <  2:755558/239‑1 (MQ=255)

            tAAAGGCGTGCAGCGTGACGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGGCACCATTTTTGGCGGCAAAGCGGGCAGCGTGTTCTTTTGCGCATCGGATATCGGCTGGGTGGTGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTATCGTTTACGAAGGATTGCCGACCTGGCC‑GGACTGCGG‑CGT‑GTGGTGGACAATCGTCGAGAAATA‑TCAgg                                                                                                                                                                                                                                     <  1:640773/239‑1 (MQ=255)

              aaGGCGTGCAGCGTGACGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCAGCGTGTTCTTTTGCGCATCGGATATCGGCTGGGTGGTGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTATCGTTTACGAAGGATTGCCGACCTGGCC‑GGACTGCGG‑CGT‑GTGGTGGACAATCGTCGAGAAATA‑TCAGG‑t                                                                                                                                                                                                                                   <  2:181601/238‑1 (MQ=255)

               aGGCGTGCAGCGGGACGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACGCCATTTTTGGCGGCAAAGCGGGCAGCGTGTTCTTTTGCGCATCGGATATCGGCTGGGTGGTGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTATCGTTTACGAAGGATTGCCGACCTGGCC‑GGACTGCGG‑CGT‑GTGGTGGACAATCGTCGAGAAATA‑TCAGG‑TTa                                                                                                                                                                                                                                 <  2:140929/239‑1 (MQ=255)

                  cGTGCAGCGTGACGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCCGCGGGTTCTTTTGCGCATCGGATATCGGGTGGGTGGTGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTATCGTTTACGAAGGATTGCCGACCTGGCC‑GGACTGCGG‑CGT‑GTGGTGGACAATCGTCGAGAAATA‑TCAGG‑TTAGc                                                                                                                                                                                                                               <  1:718800/238‑1 (MQ=255)

                  cGTGCAGCGTGACGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCAGCGTGTTCTTTTGCGCATCGGATATCGGCTGGGTGGTGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTATCGTTTACGAAGGATTGCCGACCTGGCC‑GGACTGCGG‑CGT‑GTGGTGGACAATCGTCGAGAAATA‑TCAGG‑TTAGc                                                                                                                                                                                                                               <  1:469786/238‑1 (MQ=255)

                    tGCAGCGTGACGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCAGCGTGTTCTTTTGCGCATCGGATATCGGCTGGGTGGTGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGTGGGGGGCTATCGTTTTCGCAAGATTGCCCGCCCTGCC‑GGGCTGGGG‑CGG‑GGGGGGGGCAAACGTCGGGAAAAA‑Tcgcggttggcgg                                                                                                                                                                                                                             >  2:753080/1‑228 (MQ=255)

                    tGCAGCGTGACGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCAGCGTGTTCTTTTGCGCATCGGATATCGGCTGGGTGGTGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGGTGGGGACTATCGGTTACGGAGGGTTGCCGGCCTGGGC‑GGGCTGCGG‑CGG‑GGGGGGGGCAATCGTCGGGAAATA‑TCAGG‑TTAGcccg                                                                                                                                                                                                                            >  1:1150902/1‑237 (MQ=255)

                    tGCAGCGTGACGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCAGCGTGTTCTTTTGCGCATCGGATATCGGCTGGGTGGTGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTATCGTTTACGAAGGATTGCCGACCTGGCC‑GGACTGCGG‑CGT‑GTGGTGGACAATCGTCGAGAAATA‑TCAGG‑TTAGCCgg                                                                                                                                                                                                                            >  1:887086/1‑239 (MQ=255)

                    tGCAGCGTGACGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCAGCGGGTTCTTTTGCGCATCGGATATCGGCTGGGTGGTTGGGCATTCGTATATCGTTTACGCGCCCCTGCCGGCGGGGGGGGCGCCCATCGGTTTTGAAGGCTTTCCGCACTCGCC‑CGGCTGCCG‑CCTGGGGGTGGAC‑ATCGTGCGGAACTA‑TTAGG‑GTAGcccg                                                                                                                                                                                                                            >  2:963796/1‑237 (MQ=255)

                    tGCAGCGTGACGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGAACCCATTTTTGGCGGCAAAGCGGGCAGCTTGTTCTTTTGCGCATCGGATATCGGCTGGGTGGTGGGGCATTCGTATAACGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTATCGTTTACGAAGGATTGCCGGCCTGGCC‑GGACTGCGG‑CGT‑GTGGTGGACAATCGTCGAGAAATA‑GCAGG‑TTAGCCgg                                                                                                                                                                                                                            <  2:144197/239‑1 (MQ=255)

                     gCAGCGTGACGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCAGCGTGTTCTTTTGCGCATCGGATATCGGCTGGGTGGTGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTATCGTTTACGAAGGATTGCCGGCCTGGCC‑GGGCTGCGG‑CGG‑GGGGGGGACAATCGTCGGGGAATA‑TTCGG‑TTAGCCggg                                                                                                                                                                                                                           >  1:1170317/1‑238 (MQ=255)

                     gCAGCGTGACGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCAGCGTGTTCTTTTGCGCATCGGATATCGGCTGGGTGGTGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTATCGGTTACGGAAGATTGGCGGCCTGGCC‑GGACTGCGG‑CGT‑GTGGTTGGCAATCGTCCGGGAATT‑TCAgggttgacggg                                                                                                                                                                                                                            >  1:955537/1‑230 (MQ=255)

                     gCAGCGTGACGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCAGCGTGTTCTTTTGCGCATCGGATATCGGCTGGGTGGTGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGAGGGCGGCTATCGTTTACGAAGGATTGCCGACCTGGCC‑GGACTGCGG‑CGT‑GTGGGGGACAATCGTCGGGCAATA‑TCAGG‑GTAGCAGGa                                                                                                                                                                                                                           >  1:542603/1‑239 (MQ=255)

                           tGACGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCAGCGTGTTCTTTTGCGCATCGGATATCGGCTGGGTGGTGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGGTGGGGGCTATCCTTTACGAAGGGTTGCCGAACGGGCC‑GGGCTGCGG‑CGG‑GGGGGGGACACTCGGCGGGAAATA‑TTGGG‑TTGGCCGGGTGGtct                                                                                                                                                                                                                     >  2:241300/1‑239 (MQ=255)

                                   ggggATATGCGGGGGGGCTGGCGCCCTCGAGGGACACCATTTTTGGCGGCAAGGCGGGCGGCGTGTTCTTTGGCGCATCGGATATGGGCGGGGTGGTGGGGCATTCGTATATCGTGTACGCGCCGCTGCTGGCGGGGATGGCGACTATCGTTTACGAAGGATTGCCGACCTGGCC‑GGACTGCGG‑CGT‑GTGGTGGACAATCGTCGAGAAATA‑TCAGG‑TTAGCCGGATGTTCTCA‑GCGcc                                                                                                                                                                                                             <  2:132768/236‑1 (MQ=255)

                                      gttaggCGGTGGCGCTGGCGCCTCCGATGGACACCTTTTTTGGCGGCAAAGCGGGAAGCGTGTTTTTTTGCGCATCGGATATCGGCTGGGTGGTGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTATCGTTTACGAAGGATTGCCGACCTGGCC‑GGACTGCGG‑CGT‑GTGGTGGACAATCGTCGAGAAATA‑TCAGG‑TTAGCCGGATGTTCTCA‑GCGCCG‑Ac                                                                                                                                                                                                         <  2:476594/233‑1 (MQ=255)

                                      gattggCGGTGGCGTGGGCGACCTCGATGGACACCATTGTGGGCGGCAAAGCGGGCAGCGTGTTCTTTTGCGCATCGGATATCGGCTGGGTGGTGGGGCATTCGTATAGCGGTTACGCGCCGCTGCTGGCGGGGATGGCGACTATCGTTTACGAAGGATTGCCGGCCTGGCC‑GGACTGCGG‑CGT‑GTGGTGGACAATCGTCGAGAAATA‑TCAGG‑TTAGCCGGATGTTCTCA‑GCGCCG‑Ac                                                                                                                                                                                                         <  2:469581/233‑1 (MQ=255)

                                                ggCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCAGCGTGTTCTTTTGCGCATCGGATATCGGCTGGGTGGTGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGGTGGCGACTATCGTTTACGAAGGATTGCCGACCTGGCC‑GGGCTGCGG‑CGT‑GTGGTGGACAATCGGCGGGGAATA‑TCAGG‑TTAGCCGGGTGTTCTCA‑GCGCCG‑ACCGCCA‑TTcgcg                                                                                                                                                                                             >  1:1369334/1‑239 (MQ=255)

                                                 gcgcTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCAGCGTGTTCTTTTGCGCATCGGATATCGGCTGGGTGGTGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGGTGGGGAATATCGTTTTAGAAGGAATGCCGGACCGGGC‑GGACCGAGG‑GGG‑GGTGTGGGAAAACGTGGAGGAAAT‑TCAAG‑GTTGTCGGCGGTTCTCT‑ACGCCG‑ACCGcccattgcac                                                                                                                                                                                             >  2:793489/1‑231 (MQ=255)

                                                 gcgcTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCAGCGTGTTCTTTTGCGCATCGGATATCGGCTGGGTGGTGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTATCGTTTACGAAGGATTGCCGACCTGGCC‑GGACTGCGG‑CGT‑GTGGTGGACAATCGTCGGGAAAAA‑TCAGG‑GTAGCCGGGTGTTCTCA‑GCGCCG‑ACCCCCA‑TTCGCGt                                                                                                                                                                                            >  1:1103793/1‑239 (MQ=255)

                                                 gcgcTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCAGCGTGTTCTTTTGCGCATCGGATATCGGCTGGGTGGTGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTATCGTTTACGAAGGATTGCCGACCTGGCC‑GGACTGCGG‑CGT‑GTGGGGGACAATCGTCGAGAAATA‑TCAGG‑TTAGCCGGGTGTTCTCA‑GCGCCG‑ACCGCCA‑TTCGCGt                                                                                                                                                                                            >  1:553286/1‑239 (MQ=255)

                                                                 tGGACACCATTTTTGGCGGCAAAGCGGGCAGCGTGTTCTTTTGCGCATCGGATATCGGCTGGGTGGTGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTATCGTTTACGAAGGATTGCCGACCTGGCC‑GGACTGCGG‑CGT‑GTGGTGGACAATCGTCGAGAAATA‑TCAGG‑TTAGCCGGATGTTCTCA‑GCGCCG‑ACCGCCA‑TTCGCGTGCTGAAAAAATTccc                                                                                                                                                                             <  2:887086/238‑1 (MQ=255)

                                                                         aTTTTTGGCGGCAAAGCGGGCAGCGTGTTCTTTTGCGCATCGGATATCGGCTGGGTGGTGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTATCGTTTACGAAGGATTGCCGACCTGGCC‑GGACTGCGG‑CGT‑GTGGTGGACAATCGTCGAGAAATA‑TCAGG‑TTAGCCGGGTGTTCTCA‑GCGCCG‑ACCGCCA‑TTCGCGTGCTGGAAAAATTCCCTACCGCTGa                                                                                                                                                                    >  1:29868/1‑239 (MQ=255)

                                                                           tgttGGCGGAAAAGCGGGCAGCGTGTTCTTTTGCGCATCGGATATCGGTTGGGTGGTGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTATCGTTTACGAAGGATTGCCGACCTGGCC‑GGACTGCGG‑CGT‑GTGGTGGACAATCGTCGAGAAATA‑TCAGG‑TTAGCCGGATGTTCTCA‑GCGCCG‑ACCGCCA‑TTCGCGTGCTGAAAAAATTCCCTACCGCTGaaa                                                                                                                                                                  <  2:1174181/237‑1 (MQ=255)

                                                                                  ggcAAAGCGGGCAGCGTGTTCTTTTGCGCATCGGATATCGGCTGGGTGGTGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTATCGTTTACGAAGGATTGCCGACCTGGCC‑GGACTGCGG‑CGT‑GTGGTGGACAATCGTCGAGAAATA‑TCAGG‑TTAGCCGGATGTTCTCA‑GCGCCG‑ACCGCCA‑TTCGCGTGCTGaaa                                                                                                                                                                                     >  1:73102/1‑213 (MQ=255)

                                                                                  ggcAAAGCGGGCAGCGTGTTCTTTTGCGCATCGGATATCGGCTGGGTGGTGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTATCGTTTACGAAGGATTGCCGACCTGGCC‑GGACTGCGG‑CGT‑GTGGTGGACAATCGTCGAGAAATA‑TCAGG‑TTAGCCGGATGTTCTCA‑GCGCCG‑ACCGCCA‑TTCGCGTGCTGaaa                                                                                                                                                                                     <  2:73102/213‑1 (MQ=255)

                                                                                  ggcAAAGCGGGCAGCGTGTTCTTTTGCGCATCGGATATCGGCTGGGTGGTGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTATCGTTTACGAAGGATTGCCGACCTGGCC‑GGACTGCGG‑CGT‑GTGGTGGACAATCGTCGAGAAATA‑TCAGG‑TTAGCCGGATGTTCTCA‑GCGCCG‑ACCGCCA‑TTCGCGTGCTGAAAAAATTCCCTACCGCTGAAATTCGCa                                                                                                                                                            <  1:985771/238‑1 (MQ=255)

                                                                                  ggcAAAGCGGGCAGCGTGTTCTTTTGCGCATCGGATATCGGCTGGGTGGGGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATTGCGACTCTCGTTTACCCAGGAATGCCGACCCTGCC‑GGACTGCGG‑CGT‑GTGGGGGGCCATCCGCGAGAGATA‑TCCGG‑TTAGCCGGGTGTTTTCC‑GCGCCG‑GCCCCCC‑TTCCCCGGCCGGAAAAATTCCCTACCGCTGGAATTCTCaa                                                                                                                                                           >  2:1084678/1‑239 (MQ=255)

                                                                                  ggcAAAGCGGGCAGCGTGTTCTTTTGCGCAGCGGATATCGGCTGGGTGGGGGGGCCTTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTATCGTTTACGAAGGATTGCCGACCTGGCC‑GGACTGCGG‑CGT‑GTGGTGGACAATCGTCGAGAAATA‑TCAGG‑TTAGCCGGATGTTCTCA‑GCGCCG‑ACCGCCA‑TTCGCGTGCTGAAAAAATTCCCTACCGCTGAAATTCGCa                                                                                                                                                            <  1:60135/238‑1 (MQ=255)

                                                                                                 gtgtTCTTTTGCGCATCGGATATCGGCTGGGTGGTGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTATCGTTTACGAAGGATTGCCGACCTGGCC‑GGACTGCGG‑CGT‑GTGGTGGACAATCGTCGAGAAATA‑TCAGG‑TTAGCCGGATGTTCTCA‑GCGCCG‑ACCGCCA‑TTCGCGTGCTGAAAAAATTCCCTACCGCTGAAATTCGCAAACACGAtctctc                                                                                                                                               <  2:448966/236‑1 (MQ=255)

                                                                                                 gtgtTCTTTTGCGCATCGGATATCGGCTGGGTGGTGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTATCGTTTACGAAGGATTGCCGACCTGGCC‑GGACTGCGG‑CGT‑GTGGTGGACAATCGTCGAGAAATA‑TCAGG‑TTAGCCGGATGTTCTCA‑GCGCCG‑ACCGCCA‑TTCGCGTGCTGAAAAAATTCCCTACCGCTGAAATTCGCAAACACGAtctctc                                                                                                                                               <  1:793489/236‑1 (MQ=255)

                                                                                                   gtTCTTTTGCGCATCGGATATCGGCTGGGTGGTGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGGTGGGGGCTTTCGGTTTCGGAGGATTGCCGGCCTGGCC‑GGGCTGCGG‑GGG‑GGGGGGGGGAAACGTCCGGGAAAA‑TCAGGTTTAGCCGG‑GGTTTTCA‑GCGCCC‑GCCCCCATTTCCGGGGCGGAAAAAATTTCCACCCCCGGAAATT‑GCACAAACCAtctctccctc                                                                                                                                           >  2:137418/1‑234 (MQ=255)

                                                                                                   gtTCTTTTGCGCATCGGATATCGGCTGGGTGGTGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTATCGTTTACGAAGGATTGCCGACCTGGCC‑GGACTGCGG‑CGT‑GTGGTGGACAATCGTCGAGAAATA‑TCAGG‑TTAGCCGGATGTTCTCA‑GCGCCG‑ACCGCCA‑TTCGCGTGCTGAAAAAATTCCCTACCGCTGAAATTCGCAAACACGATCTCTCGTCGc                                                                                                                                          <  1:1028603/239‑1 (MQ=255)

                                                                                                       ttttGCGCAGCGGATATCGGCGGGGTGGTGGGTCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTATCGTTTACGAAGGATTGCCGACCTGGCC‑GGACTGCGG‑CGT‑GTGGTGGACAATCGTCGAGAAATA‑TCAGG‑TTAGCCGGATGTTCTCA‑GCGCCG‑ACCGCCA‑TTCGCGTGCTGAAAAAATTCCCTACCGCTGAAATTCGCAAACACGATCTCTCGTCGCTGGa                                                                                                                                      <  2:115180/239‑1 (MQ=255)

                                                                                                            cgcATCGGATATCGGCTGGGTGGTGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGGTGGCGACTATCGTTTACGAAGGATTGCCGACCTGGCC‑GGAATGCGG‑CGT‑GTGGGGGACAATCGTCGGGAAATA‑TCAGG‑GTTGCCGGGTGTTCTCA‑GCGCCG‑ACCGCCA‑TTCGCGTGCTGAAAAAATTTCCTACCGCCGAAATTCTCACACACGAACCCTCGTCGCTGGAAGTg                                                                                                                                  >  2:348512/1‑238 (MQ=255)

                                                                                                            cgcATCGGATATCGGCTGGGTGGTGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGAATATCGTTTACGAAGGATTGCCGACCTGGCC‑GGACTGCGG‑CGT‑GTGGGGGACAATCGTCGAGAAATA‑TCAGG‑TTAGCCGGATGTTCTCA‑GCGCCG‑ACCGCCA‑TTCGCGTGCTGAAAAAATTTCCTACCGCTGAAATTCGCAAACACGATCTCTCGTCGCTGGAAGt                                                                                                                                   >  1:115180/1‑237 (MQ=255)

                                                                                                                 cggaattCGGCTGGGTGGTGGGGCGCTCGATTATGGTTTACGCGCCGCTGCTGGGGGGGTTGGTGACTATCGTTTACGAAGGCTTGCCGCCGTGGCC‑GGGTTGCGG‑CGT‑GTGGTGGACAATCGTCGAGAAAAA‑TCAGG‑TTAGCCGGATGTTCTCA‑GCGCCG‑CCCGCCA‑TTCGCGTGCTGAAAAAATTCCCTACCGCTGAAATTCGCAAACACGATCTCTCGTCGCTGGAAGTGCTCTAt                                                                                                                            <  2:955537/233‑1 (MQ=255)

                                                                                                                             gggTGGTGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTATCGTTTACGAAGGATTGCCGACCTGGCC‑GGACTGCGG‑CGT‑GTGGTGGGCAATCGTCGAGAAATA‑TCAGG‑TTAGCCGGATGTTCTCA‑GCGCCG‑ACCGCCA‑TTCGCGTGCTGAAAAAATTCCCTACCGCTGCAATTCGCCAACACGATCTCTCGTCGCTGGGAGGGCTCTATCTGGCTTgaga                                                                                                                 >  2:96249/1‑238 (MQ=255)

                                                                                                                             gggAGGTGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGGTGGCGAGTATCGTTTAAGAAGGATTGCCGCACCGGCC‑GGGCTGCGG‑GGG‑GTGGGGGACAATCGGCGGGAAATA‑TCAGG‑GTTGCCGGCTGTTTTTC‑GGGGCG‑ACCGCCA‑CTCCCGTGCTGACAAAATTCCCTACCCACGAAATTTGCAAACACGCACTCTCCTTGCTGGAAGTGCTTCATatggctgtcga                                                                                                                 >  2:63301/1‑234 (MQ=255)

                                                                                                                              ggtggtGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGGTGGCGACTATCGTTTACGCAGGATTGCCGGCCTCGCC‑GGACCGCGG‑CGG‑GGGGGGGGCAAACCTCGGGGAATACTCGGG‑TT‑GCCGGATTGTTTCA‑GCGCCGAACCGCCT‑TCCGCG‑GCTTGAAAAATTCCCTACCCGCGAAATTGGCCAACCCGATCTCCGCTCGCGTGAAGGGCTCTCTtcggctggggca                                                                                                                >  2:122710/1‑234 (MQ=255)

                                                                                                                              ggtgggGGGGCAGTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTATCGTTTACGAAGGATTGCCGACCTGGCC‑GGACTGCGG‑CGT‑GTGGTGGACAATCGTCGAGAAATA‑TCAGG‑TTAGCCGGATGTTCTCA‑GCGCCG‑ACCGCCA‑TTCGCGTGCTGAAAAAATTCCCTACCGCTGAAATTCGCAAACACGATCTCTCGTCGCTGGAAGTGCTCTATct                                                                                                                          <  1:545029/228‑1 (MQ=255)

                                                                                                                              gggggtggGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTATCGTTTACGAAGGATTGCCGACCTGGCC‑GGACCGCGG‑GGG‑GTGGGGGGCAATCGTCGAGGAAAA‑TCAGG‑GTAGACGGGTGTTTTCA‑GCGCCG‑ACCGCCC‑TTAGCGTGCTGGAAAAAATCCCTACCGCTGAAATTCGCAAACACGAACTCTCGTCGCTGGAGGAGGCCTATctggcgtgggaa                                                                                                                >  2:517477/4‑231 (MQ=255)

                                                                                                                                  gtggGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTATCGTTTACGAAGGATTGCCGACCTGGCC‑GGACTGCGG‑CGT‑GTGGTGGACAATCGTCGAGAAATA‑TCAGG‑TTAGCCGGATGTTCTCA‑GCGCCG‑ACCGCCA‑TTCGCGTGCTGAAAAAATTCCCTACCGCTGAAATTCGCCAAACCGATCTCTCCTCGCTGGAAGTGCCCTATCTGGCTGGAGAAcc                                                                                                              >  1:358995/1‑236 (MQ=255)

                                                                                                                                   tggGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGGTGGCGACTATCGTTTACGAAGGGTTGCCGACCTGGCC‑GGACTGCGG‑CGT‑GTGGGGGACAATCGTCGAGAAATA‑TCAGG‑GTAGCCGGGTGTTCTCA‑GCGCCG‑GCCGCCA‑TTCGCGTGCTGAAAAAATTCCCTACCGCTGAAATTCGCAAACACGATCTCTCGTCGCTGGAAGTGCTCTATCTGGCTGGAGAACCGcgg                                                                                                          >  2:266781/1‑237 (MQ=255)

                                                                                                                                        cATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGGTGGCGACTATCGTTTACGAAGGATTGCCGACCTGGCC‑GGACTGCGG‑CGT‑GTGGTGGGCAATCGTCGAGAAATA‑TCAGG‑TTAGCCGGATGTTCTCA‑GCGCCG‑ACCGCCA‑TTCGCGTGCTGAAAAAATTCCCTACCGCTGAAATTCGCAAAAACGATCTCTCGGCGCTGGCAGTGCTCTATCTGGCTGGGGAAACGCTGGaggg                                                                                                     >  2:1169728/1‑236 (MQ=255)

                                                                                                                                        cATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTATCGTTTACGAAGGATTGCCGACCTGGCC‑GGACTGCGG‑CGT‑GTGGTGGACAATCGTCGAGAAATA‑TCAGG‑TTAGCCGGGTGTTCTCC‑GCGCCG‑ACCGCCC‑TTCGCGTGCTGGAAAAATTCCCTCCCGCTGAAATTCGCAAACACGATCTCTCGTCGCTGGCAGTGCTCTTTCTGGCTTGCGAAcctttctcttt                                                                                                     >  2:545029/1‑230 (MQ=255)

                                                                                                                                               atgtCGTTTAGGGGCGGTTGCTGGGGGGGGTGGCGATTATCTTTTACGAGGGTTTCCGGGCTGGGCG‑GGCCTGCGG‑GGG‑GTGGGGGACAATCGTCGAAAAATA‑TCAGG‑TTAGCCGGATG‑TCTCAGGCCCGG‑CCCGCCA‑TTCGCGTGCTGAAAAAATTCCCTACCGCTGAAATTCGCAAACACGATCTCTCGTCGCTGGAAGTGCTCTATCTGGCTGGAGAACCGCTGGACGAGCCGAc                                                                                               <  2:1043650/235‑1 (MQ=255)

                                                                                                                                               atatCGTTTACGCGCCGCTGCTGGCGGGGAGGGCGACTATCGTTTACGAAGGATTGCCGACCTGGCC‑GGACTGCGG‑CGT‑GTGGTGGACAATCGTCGAGAAATA‑TCAGG‑TTAGCCGGATGTTCTCA‑GCGCCG‑ACCGCCA‑TTCGCGTGCTGAAAAAATTCCCTACCGCTGAAATTCGCAAACACGATCTCTCGTCGCTGGAAGTGCTCTATCTGGCTGGAGAACCGCTGGACGAGCCGAc                                                                                               <  1:137418/238‑1 (MQ=255)

                                                                                                                                                tatCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTATCGTTTACGAAGGATTGCCGACCTGGCC‑GGACTGCGG‑CGT‑GTGGTGGACAATCGTCGAGAAATA‑TCAGG‑TTAGCCGGATGTTCTCA‑GCGCCG‑ACCGCCA‑TTCGCGTGCTGAAAAAATTTCCTACCGCTGAAATTCGCAAACACGATCTCTCGTCGCTGGAAGTGCTCTATCTGGCTGGAGAACCGCTGGACGAGCCGAcc                                                                                              >  1:774453/1‑238 (MQ=255)

                                                                                                                                                 atCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTCTCGTTTTAGAAGGATTTCCGACCTGGCC‑GGACTGCGG‑CGT‑GGGGGGGACAATAGTCGAGAAATA‑TCAGG‑TTAGCCGGGTGGTCTCA‑GCGCCG‑GCCGCCA‑TTCGCGGGCTGAAAAAATTCCCTACCCCTGGAATTCCCCAACACCATCTCTCGTCTGTGGAAGTGGCCCATCTGGGCGGTGAAACGCTGGGCGAGCCGcccc                                                                                             >  2:173509/1‑237 (MQ=255)

                                                                                                                                                  tCGTTTAGGCGCCGCTGCTGGCGGGGATGGCGACTATCGTTTACGAAGGATTGCCGCCCTGGCG‑GGACGGCGG‑CGT‑GTGGTGGACAATCGTCGAGAAATA‑TCAGG‑TTAGCCGGATGTTCTCA‑GCGCCG‑ACCGCCA‑TTCGCGTGCTGAAAAAATTCCCTACCGCTGAAATTCGCAAACACGATCTCTCGTCGCTGGAAGTGCTCTATCTGGCTGGAGAACCGCTGGACGAGCCGACCGcc                                                                                           <  1:753080/239‑1 (MQ=255)

                                                                                                                                                     tttACGCGCCGCTGCTGGCGGGGGGGGCGGCTATCGTTTACGAAGGATTGCCGACCCGGCC‑GGACTGCGG‑CGT‑GGGGGGGAAAATCGTCGAGAAATA‑TCAGG‑GTAGCCGGGTGTTCTCA‑GCGCCG‑ACCGCCA‑TTCGCGGGCTGAAAAAATTCCCTACCGCTGAAATTCCCAAACAACATCTCTCGTCGCTGGAAGTGCTCTCTCTGGCTGGGGAACCGCTGGACGCGCCCACCCCCCGt                                                                                        >  2:1256575/1‑239 (MQ=255)

                                                                                                                                                     tttACGCGCCGCTGCTGGCGGGGATGGCGACTATCGTTTACGAAGGATTGCCGACCTGGCC‑GGACTGCGG‑CGT‑GTGGTGGACAATCGTCGAGAAATA‑TCAGG‑TTAGCCGGGTGTTCTCA‑GCGCCG‑ACCGCCA‑TTCGCGTGCTGAAAAAATTCCCTACCGCTGAAATTCGCAAACACGATCTCTCGTCGCTGGAAGTGCTCTATCTTGCTGGAGAACCGCTGGACGAGCCGACCGCCAGt                                                                                        >  1:327712/1‑239 (MQ=255)

                                                                                                                                                     tttACGCGCCGCTGCTGGCGGGGATGGCGACTATCGTTTACGAAGGATTGCCGACCTGGCC‑GGACTGCGG‑CGT‑GTGGTGGACAATCGTCGAGAAATA‑TCAGG‑TTAGCCGGATGTTCTCA‑GCGCCG‑ACCGCCC‑TTCCCGTGCTGAAAAAATTCCCTACCGCTGAAATTCGCAAACACGATCTCTCGTCGCTGGAAGTGGTCTATCTGGCTTGAGGAACGCTGGACGAGCCGACCGCCAGt                                                                                        >  2:1407156/1‑239 (MQ=255)

                                                                                                                                                     tttACGCGCCGCTGCTGGCGGGGATGGCGACTATCGTTTACGAAGGATTGCCGACCTGGCC‑GGACTGCGG‑CGT‑GTGGTGGACAAACGTCGAGAAATA‑TCAGG‑TTAGCCGGATGTTCTCA‑GCGCCG‑AACGCCA‑TTCGCGTGCTGGAAAAATTCCCTACCGCTGAAATTCGCAAACACGATCTCTCGGCGCTGGAAGTGCTCTATCTGGCTGGAGAACCGCCGGAGGAGCCGACCCCCCGt                                                                                        >  1:1085762/1‑239 (MQ=255)

                                                                                                                                                     tttACGCGCCGCTGCTGGCGGGGATGGCGACTATCGTTTACGAAGGATTGCCGACCTGGCC‑GGACTGCGG‑CGT‑GTGGGGGACAATCGTCGAGAAATA‑TCAGG‑TTAGCCGGGTGTTCTCA‑GGGCCG‑ACCGCCA‑TTCGCGTGCTGGAAAAATTCCCTACCGCTGAAATTCGCAAACACGATCTCTCGTCGCTGGAAGGGCTCTATCTGGCTGGGGAACCGCTGGAAGAGCCGACCGCCa                                                                                          >  2:1323198/1‑237 (MQ=255)

                                                                                                                                                     tttACGCGCCGCTGCTGGCGGGGATGGCGACTATCGTTTACGAAGGATTGCCGACCTGGCC‑GGAATGCGG‑CGG‑GTGGTGGGCAATCGTCGAAAAAAA‑TCAAG‑TTAGCCGGATGTTCTCA‑GCGCCG‑ACCGCCC‑TTCGCGTGCTGAAAAAATTCCCTACAGGTGAAAATTGCCAAAACGATCTCCCGTCCCTGGAAGGGCTCTATCTGGCTGGGGGACCGCGGGACGAGCCGACCGCCacg                                                                                        >  2:1011485/1‑237 (MQ=255)

                                                                                                                                                       tACGCGCCGCTGCTGGCGGGGATGGCGACTATCGTTTACGAAGGATTGCCGACCTGGCC‑GGACTGCGG‑CGT‑GTGGTGGACAATCGTCGAGGAATA‑TCAGG‑TTAGCCGGATGTTCTCA‑GCGCCG‑ACCGCCA‑TTCGCGTGCTGAAAAAATTCCCTACCGCTGAAATTCGCAAACACGATCTCTCGTCGCTGGAAGAGCTCTATCTGGCTGGAGGACCGCTGGACGAGCCGACCGCCAGTTg                                                                                      >  2:1272689/1‑239 (MQ=255)

                                                                                                                                                       tACGCGCCGCTGCTGGCGGGGATGGCGACTATCGTTTAAGAAGGATTGCCGACCTGGCC‑GGACTGCGG‑CGT‑GTGGTGGACAATCGTCGAGAAATA‑TCAGG‑TTAGCCGGATGTTCTCA‑GCGCCG‑GCCGCCA‑TTCGCGTGCTGAAAAAATTCCCTACCGCTGAAATTCGCAAACACGATCTCTCGTCGCTGGAAGGGCTCTATCTGGCTGGAGAACCGCTGGACGAGCCGACCGCCAGttt                                                                                      >  1:956813/1‑238 (MQ=255)

                                                                                                                                                        aCGCGCCGCTGCTGGCGGGGATGGCGACTATCGTTTACGAAGGATTGCCGACCTGGCC‑GGACTGCGG‑CGT‑GTGGTGGACAATCGTCGAGAAATA‑TCAGG‑TTAGCCGGATGTTCTCC‑GCGCCG‑AACGCCA‑TTCGCGTGCTGAAAAAATTCCCTACCGCTGAAATTCGCAAACACGATCTCTCGTCGCTGGGAGTGCTCTATCTGGCTGGAGAACCGCTGGACGAGCCGCCCCCCAGTTgg                                                                                     >  1:492781/1‑239 (MQ=255)

                                                                                                                                                        aCGCGCCGCTGCGGGCGGGGATGGCGACTATCGTTTACGAAGGATTGCCGACCTGGCC‑GGACTGCGG‑CGT‑GTGGTGGACAATCGTCGAGAAATA‑TCAGG‑TTAGCCGGATGTTCTCA‑GCGCCG‑ACCGCCA‑TTCGCGTGCTGAAAAAATTCCCTACCGCTGAAATTCGCAAACACGATCTCTCGTCGCTGGAAGTGCTCTATCTGGCTGGAGAACCGCTGGACGAGCCGACCGCCAGTTgg                                                                                     <  1:96249/239‑1 (MQ=255)

                                                                                                                                                        aCGCCCCGCTGGTGGTGGGGATGGCGACTAGCGTGACCGAGGGATTG‑CGTCCCGGCCTGGAGTGGGG‑CGG‑GGGGTGCACACTCGGGGAAAAATT‑TCGGG‑AAAGCATGCGGTCCGAT‑GCCCCG‑ACCGCCA‑TTGGCGGCCTGAAAAAATTCCCTACCGCTGAAATTCGCAAACACGATCGCTCGTCGCTGGAAGTGCTCTATCGGGCTGGATATCCGCTGGACGAGCCGCCCGCCAGTTgg                                                                                     <  2:1097892/239‑1 (MQ=255)

                                                                                                                                                          gcgcCGCTGCTGGGGGGGATGGCGATTATCGTTTAGGAAGGATTGCCGACCGGCCC‑GGACGGCGG‑CGT‑GTGGTGGACAATCGTCAAGAAATA‑TAAGG‑TTAGCCGGATGTCCTAA‑GCGCCG‑ACCGCCA‑TTCGCGTGCTGAAAAAATTCCCTACCGCTGAAATTCGCAAACACGATCTCTCGTCGCTGGAAGTGCTCTATCTGGCTGGAGAACCGCTGGACGAGCCGACCGCCAGTTGGGt                                                                                   <  2:38526/239‑1 (MQ=255)

                                                                                                                                                          gccccgcGGGTGGCGGGGATGGCGACTATCGTTTACGAAGGATTGCCGACCTGGCC‑GGACTGCGG‑CGT‑GGGGTGGACAATCGTCGAGAAATA‑CCAGG‑TTAGCCGGATGTTCTCA‑GCGCCG‑ACCGCCA‑TTCGCGTGCTGAAAAAATTCCCTACCGCTGAAATTCGCAAACACGATCTCTCGTCGCTGGAAGTGCTCTATCTGGCTGGAGAACCGCTGGACGAGCCGACCGCCAGTTGGGt                                                                                   <  1:1084678/236‑1 (MQ=255)

                                                                                                                                                              cgcTGCTGGCGGGGAGGGCGACTATCGTTTACGAAGGATTGCCGGCCCGGCC‑GGACCGCGG‑CGG‑GTGGTGGACAATCCTCGAGAAATA‑TCAGG‑TTTGCCGGGTCTTCTCA‑GCCCCC‑ACCGCCC‑TTCGCGTGCTGAAAAAATTCCCTACCCCTGAAATTCCCAAACACCAACCCTCGTCGCTGGTAGTGCCACATCCTGGTGGGGCACCGCTGGAAGAGCCGACCGccccatgggcgag                                                                                >  2:309226/1‑227 (MQ=255)

                                                                                                                                                                     gggggggTTGCCGACTGTCGTTAACGAAGGATGGCCGACCTGGCC‑GGGCTGGGG‑CGG‑GTGGTGGAGAATCGTGGAAAAAGT‑CCAGT‑TTAGCCGGATGTTCCCG‑GCGCCG‑ACCGCCA‑TTCGCGTGCTGAAAAAATTCCCTACCGCTGAAATTCGCAAACACGATCTCTCGTCGCTGGAAGTGCTCTATCTGGCTGGAGAACCGCTGGACGAGCCGACCGCCAGTTGGGTGAGCAATACg                                                                         <  2:898179/235‑1 (MQ=255)

                                                                                                                                                                           gATGGCGACTATCGTTTTCGAAGGATTGCCGACCTGGCC‑GGACTGCGG‑CGT‑GTGGTGGACAATCGTCGGGAAATA‑TCAGG‑TTAGCCGGATGTTCTCA‑GCGCCG‑ACCGCCA‑TTCGCGTGCTGAAAAAATTCCCTACCGCTGAAATTCGCAAACACGATCTCTCGTCGCGGGAAAAGCTCTATCTGCCTGGGGAACCGCTGGACGAGCCCCCCCCCCAGTGGGTGCGCAATACGCTGGAtg                                                                  >  2:865327/1‑239 (MQ=255)

                                                                                                                                                                              ggCGACTATCGTTTACGAAGGATTGCCGACCTGGCC‑GGACTGCGG‑CGT‑GTGGTGGACAATCGTCGAGAAATA‑TCAGG‑TTAGCCGGATGTTCTCA‑GCGCCG‑ACCGCCA‑TTCGCGTGCTGAAAAAATTCCCTACCGCTGAAATTCGCAAACACGATCTCTCGTCGCTGGAAGTGCTCTATCTGGCTGGAGAACCGCTGGACGAGCCGACCGCCAGTTGGGTGAGCAATACGCTGGAtgtg                                                                >  1:1302313/1‑238 (MQ=255)

                                                                                                                                                                                cGACTATCGTTTACGAAGGATTGCCGACCTGGCC‑GGACTGCGG‑CGT‑GTGGTGGACAATCGTCGAGAAATA‑TCAGG‑TTAGCCGGATGTTCTCA‑GCGCCG‑ACCGCCA‑TTCGCGTGCTGAAAAAATTCCCTACCGCTGAAATTCGCAAACACGATCTCTCGTCGCTGGAAGTGCTCTATCTGGCTGGAGAACCGCTGGACGAGCCGAGCGCCAGTTGGGTGAGCCATACGCTGGAtggtccg                                                             >  2:1300844/1‑234 (MQ=255)

                                                                                                                                                                                   tttgcGTTGAGGAAGGATTGCCGCCCTGGCC‑GGGCGGGGG‑GGT‑GGGGGGGCAAATGGGCGAGAAATA‑CCGGT‑TTAGCCGAATGTTCTCA‑GCGCGC‑ACCGCCA‑TTCGCTTGCTGAAAAAATTCCCTACCGCTGAAATTCGCAAACACGATCTCTCGTCGCTGGAAGTGCTCTATCTGGCTGGAGAACCGCTGGACGAGCCGACCGCCAGTTGGGTGAGCAATACGCTGGATGTGCCGGt                                                           <  2:1124115/234‑1 (MQ=255)

                                                                                                                                                                                         tttACGAAGGATTGCCGACCTGGCC‑GGACTGCGG‑CGT‑GTGGTGGACAATCGTCGAGAAATA‑TCAGG‑TTAGCCGGATGTTCTCA‑GCGCCG‑ACCGCCA‑TTCGCGTGCTGAAAAAATTCCCTACCGCTGAAATTCGCAAACACGATCTCTCGTCGCTGGAAGTGCTCTATCTGGCTGGAGAACCGCTGGACGAGCCGACCGCCAGTTGGGTGAGCAATACGCTGGATGTGCCGGTCATCGAc                                                    >  2:107608/1‑239 (MQ=255)

                                                                                                                                                                                         ttaaCGATGGATTGCCGCCCTGGGC‑GGCCGGGGG‑CGG‑GGGGTGGAAAATCGTCGGAAAAAT‑TCGGG‑TTCGCCGGATGTTCTCA‑GCGCCG‑CCCGCCA‑TTCGCGTGCTGAAAAAATTCCCTACCGCTGAAATTCGCAAACACGATCTCTCGTCGCTGGAAGTGCTCTATCTGGCTGGAGAACCGCTGGACGAGCCGACCGCCAGTTGGGTGAGCAATACGCTGGATGTGCCGGTCATCGa                                                     <  2:542603/235‑1 (MQ=255)

                                                                                                                                                                                            aCGAAGGATTGCCGCCCGGGCC‑GGACTGCGG‑CGT‑GTGGTGGACAATCGTCGAGAAATA‑TCAGG‑TTAGCCGGATGTTCTCA‑GCGCCG‑ACCGCCA‑TTCGCGTGCTGAAAAAATTCCCTACCGCTGAAATTCGCAAACACGATCTCTCGTCGCTGGAAGTGCTCTATCTGGCTGGAGAACCGCTGGACGAGCCGACCGCCAGTTGGGTGAGCAATACGCTGGATGTGCCGGTCATCGACAac                                                 <  1:1023230/239‑1 (MQ=255)

                                                                                                                                                                                             cGAAGGATTGCCCGCCCGGCC‑CGGATGTGGTCGT‑TGGGTGGAAATTCGCCGAAAAATT‑TCAGT‑TGACCCGGATGTTCCCG‑GCCCCA‑CCCGCCA‑TTCGCGTGCTGAAAAAATTCCCTACCGCTGAAATTCGCAAACACGATCTCTCGTCGCTGGAAGTGCTCTATCTGGCTGGAGAACCGCTGGACGAGCCGACCGCCAGTTGGGTGAGCAATACGCTGGATGTGCCGGTCATCGACAac                                                 <  2:1150902/239‑1 (MQ=255)

                                                                                                                                                                                              gAAGGATTGCCGACCTGGCC‑GGACTGCGG‑CGT‑GTGGTGGACAATCGTCGAGAAATA‑TCAGG‑TTAGCCGGATGTTCTCA‑GCGCCG‑ACCGCCA‑TTCGCGTGCTGAAAAAATTCCCTACCGCTGAAATTCGCAAACACGATCTCTCGTCGCTGGAAGTGCTCTATCTGGCTGGAGAACCGCTGGACGAGCCGACCGCCAGTTGGGTGAGCAATACGCTGGATGTGCCGGTCATCGACAact                                                >  1:1372326/1‑238 (MQ=255)

                                                                                                                                                                                                aGGATTGCCGACCTGGCC‑GGACTGCGG‑CGT‑GTGGTGGGCAATCGTCGAGAAATA‑TCAGG‑TTAGCCGGATGTTCTCA‑GCGCCG‑ACCGCCA‑TTCGCGTGCTGAAAAAATTCCCTACCGCTGAAATTCGCAAACACGATCTCTCGTCGCTGGAAGTGCTCTATCTGGCTGGAGAACCGCTGGACGAGCCGACCGCCAGTTGGGTGAGCAATACGCTGGATGTGCCGGTCATCGACAact                                                >  2:1402110/1‑236 (MQ=255)

                                                                                                                                                                                                aGGATTGCCGACCTGGCC‑GGACTGCGG‑CGT‑GTGGTGGACAATCGTCGAGAAATA‑TCAGG‑TTAGCCGGATGTTCTCA‑GCGCCG‑ACCGCCA‑TTCGCGTGCTGAAAAAATTCCCTACCGCTGAAATTCGCAAACACGATCTCTCGTCGCTGGAAGTGCTCTATCTGGCTGGAGAACCGCTGGACGAGCCGACCGCCAGTTGGGTGAGCAATACGCTGGATGTGCCGGTCATCGACAact                                                >  1:228181/1‑236 (MQ=255)

                                                                                                                                                                                                    ttGCCGACCTGGCC‑GGACTGCGG‑CGT‑GTGGTGGACAATCGTCGAGAAATA‑TCAGG‑TTAGCCGGATGTTCTCA‑GCGCCG‑ACCGCCA‑TTCGCGTGCTGAAAAAATTCCCTACCGCTGAAATTCGCAAACACGATCTCTCGTCGCTGGAAGTGCTCTATCTGGCTGGAGAACCGCTGGACGCGCCGACCGCCAGTTGGGTGAGCAATACGCTGGATGTGCCGGTCATCGACCACTACTGGCa                                         >  1:581044/1‑239 (MQ=255)

                                                                                                                                                                                                          aCCTGGCC‑GGACTGCGG‑CGT‑GTGGTGGACAATCGTCGAGAAATA‑TCAGG‑TTAGCCGGATGTTCTCA‑GCGCCG‑ACCGCCA‑TTCGCGTGCTGAAAAAATTCCCTACCGCTGAAATTCGCAAACACGATCTCTCGTCGCTGGAAGTGCTCTATCTGGCTGGAGAACCGCTGGACGAGCCGACCGCCAGTTGGGTGAGCAATACGCTGGATGTGCCGGTCATCGACAact                                                >  1:1197941/1‑226 (MQ=255)

                                                                                                                                                                                                            cTGGCC‑GGACTGCGG‑CGT‑GTGGTGGACAATCGTCGAGAAATA‑TCAGG‑TTAGCCGGATGTTCTCA‑GCGCCG‑ACCGCCA‑TTCGCGTGCTGAAAAAATTCCCTACCGCTGAAATTCGCAAACACGATCTCTCGTCGCTGGAAGTGCTCTATCTGGCTGGAGAACCGCTGGACGAGCCGACCGCCAGTTGGGTGAGCAATACGCTGGATGGGCCGGTCATCGACAACTACTGGCAGACCGaa                                  >  2:381887/1‑238 (MQ=255)

                                                                                                                                                                                                              ggCC‑GGACTGCGG‑CGT‑GTGGTGGACAATCGTCGAGAAATA‑TCAGG‑TTAGCCGGATGTTCTCA‑GCGCCG‑ACCGCCA‑TTCGCGTGCTGAAAAAATTCCCTACCGCTGAAATTCGCAAACACGATCTCTCGTCGCTGGAAGTGCTCTATCTGGCTGGAGAACCGCTGGACGAGCCGACCGCCAGTTGGGTGAGCAATACGCTGGATGTGCCGGTCATCGACAACTACCGGCAGACCGAATc                                >  2:667997/1‑238 (MQ=255)

                                                                                                                                                                                                               gCC‑GGACTGCGG‑CGT‑GTGGTGGACAATCGTCGAGAAATA‑TCAGG‑TTAGCCGGATGTTCTCA‑GCGCCG‑ACCGCCA‑TTCGCGTGCTGAAAAAATTCCCTACCGCTGAAATTCGCAAACACGATCTCTCGTCGCTGGAAGTGCTCTATCTGGCTGGAGAACCGCTGGACGAGCCGACCGCCAGTTGGGTGAGCAATACGCTGGATGTGCCGGTCATCGACAACTACTGGCAGACCGAATCCg                              >  1:549310/1‑239 (MQ=255)

                                                                                                                                                                                                                     aCTGCGG‑CGT‑GGGGTGGACAATCGTCGAGAAATA‑TCAGG‑TTAGCCGTATGTCCTCA‑GCGCCG‑CCCGCCA‑TTCGCGTGCTGAAAAAATTCCCTACCGCTGAAATTCGCAAACACGATCTCTCGTCGCTGGAAGTGCTCTATCTGGCTGGAGAACCGCTGGACGAGCCGACCGCCAGTTGGGTGAGCAATACGCTGGATGTGCCGGTCATCGACAACTACTGGCAGACCGAATCCGGCTg                          <  1:173509/238‑1 (MQ=255)

                                                                                                                                                                                                                      cggcgg‑cgT‑GGGGTGGACAACCGGCGAAAAATA‑TCAGG‑TTGGCCGTATGTTCCCA‑GCGCAG‑ACCGCCA‑TTCGCGTGCTGAAAAAATTCCCTACCGCTGAAATTCGCAAACACTATCTCTCGTCGCTGAAAGTGCTCTATCTGGCTGGAGAACCGCTGGACGAGCCGACCGCCAGTTGGGTGAGCAATACGCTGGATGTGCCGGTCATCGACAACTACTGGCAGACCGAATCCGGCTGGc                        <  1:1021081/237‑1 (MQ=255)

                                                                                                                                                                                                                      cTGCGG‑CGT‑GTGGTGGACAATCGTCGAGAAATA‑TCAGG‑TTAGCCGGATGTTCTCA‑GCGCCG‑ACCGCCA‑TTCGCGTGCTGAAAAAATTCCCTACCGCTGAAATTCGCAAACACGATCTCTCGTCGCTGGAAGTGCTCTATCTGGCTGGAGAACCGCTGGACGAGCCGACCGCCAGTTGGGTGAGCAATACGCTGGATGTGCCGGTCATCGACAACTACTGGCAGACCGAATCCGGCTGGc                        <  1:107608/239‑1 (MQ=255)

                                                                                                                                                                                                                         ggcggctgtgggtggAAAACCGGCGAGAAATT‑TCGGG‑TTGCCCGGATGTTCCAA‑GCGCCG‑CCCGCCA‑TTCGCGTGCTGAAAAAATTCCCTACCGCTGAAATTCGCAAACACGAGCTCTCGTCGCTGGAAGTGCTCTATCTGGCTGGAGAACCGCTGGACGAGCCGACCGCCAGTTGGGTGAGCAATACGCTGGATGTGCCGGTCATCGACAACTACTGGCAGACCGAATCCGGCTGGc                        <  2:358995/228‑1 (MQ=255)

                                                                                                                                                                                                                                      ggaaaacggtggagaAATA‑TCAGG‑TTAGCCGGATGTTCCCA‑GCGCCG‑CCCGCCA‑TTCGCGTGCTGAAAAAATTCCCTACCGCTGAAATTCGCAAACACGATCTCTCGTCGCTGGAAGTGCTCTATCTGGCTGGAGAACCGCTGGACGAGCCGACCGCCAGTTGGGTGAGCAATACGCTGGATGTGCCGGTCATCGACAACTACTGGCAGACCGAATCCGGCTGGCCGATTATGGCGAt           <  2:1372326/227‑1 (MQ=255)

                                                                                                                                                                                                                                           agcgtcgAAAAATA‑ACAGG‑TTAGCCGGATGTCCTCA‑GCGCCG‑CCCGCCA‑TTCGCGTGCTGAAAAAATTCCCTACCGCTGAAATTCGCAAACACGATCTCTCGTCGCTGGAAGTGCTCTATCTGGCTGGAGAACCGCTGGACGAGCCGACCGCCAGTTGGGTGAGCAATACGCTGGATGTGCCGGTCATCGACAACTACTGGCAGACCGAATCCGGCTGGCCGATTATGGCGATTGCTcg     <  2:553286/237‑1 (MQ=255)

                                                                                                                                                                                                                                           aTCGTCGAGAAATA‑TCAGT‑TTAGCCGGATGTTCTCA‑GCGCCG‑ACCGCCA‑TTCGCGTGCTGAAAAAATTCCCTACCGCTGAAATTCGCAAACACGATCTCTCGTCGCTGGAAGTGCTCTATCTGGCTGGAGAACCGCTGGACGAGCCGACCGCCAGTTGGGTGAGCAATACGCTGGATGTGCCGGTCATCGACAACTACTGGCAGACCGAATCCGGCTGGCCGATTATGGCGATTGCTcg     <  1:348512/239‑1 (MQ=255)

                                                                                                                                                                                                                                            tcgtcgAGAAATA‑TCATG‑AAAAACGGATGTTCTCA‑GCGCCG‑ACCGCCA‑TTCGCGGGCTGAAAAAATACACTACCGCTGAAATTCGCAACCACGATCTCTCGCCGCTGGAGATGCTCTATCTGGATGGAGAACCGCTGGACGTGCGGACCGCCGCGTGGCTGAGCAATCCGCTGGATGTGCCGGTCATCGACAACGACAGGCAGACCGAAGACTGCTGGCCGATTCTGGCCATGGCTcgc    >  2:837985/1‑239 (MQ=255)

                                                                                                                                                                                                                                            tcgtcgAGAAATA‑TCAGG‑TTAGCCGGATGTTCTCA‑GCGCCG‑ACCGCCA‑TTCGCGTGCTGAAAAAATTCCCTACCGCTGAAATTCGCAAACACGATCTCTCGTCGCTGGAAGTGCTCTATCTGGCTGGAGAACCGCTGGACGAGCCGACCGCCAGTTGGGTGAGCAATACGCTGGATGTGCCGGTCATCGACAACTACTGGCAGACCGAATCCGGCTGGCCGATTCTGGCGATTGCTcgc    >  1:997650/1‑239 (MQ=255)

                                                                                                                                                                                                                                            tcgtcgAGAAATA‑TCAGG‑TTAGCCGGATGTTCTCA‑GCGCCG‑ACCGCCA‑TTCGCGTGCTGAAAAAATTCCCTACCGCTGAAATTCGCAAACACGATCTCTCGTCGCTGGAAGTGCTCTATCTGGCTGGAGAACCGCTGGACGAGCCGACCGCCAGTTGGGTGAGCAATACGCTGGATGTGCCGGTCATCGACAACTACTGGCAGACCGAATCCGGCTGGCCGATTATGGCGATTGCTcgc    >  1:900322/1‑239 (MQ=255)

                                                                                                                                                                                                                                            tcgtcgAGAAATA‑TCAGG‑TTAGCCGGATGTTCTCA‑GCGCCG‑ACCGCCA‑TTCGCGTGCTGAAAAAATTCCCTACCGCTGAAATTCGCAAACACGATCTCTCGTCGCTGGAAGTGCTCTATCTGGCTGGAGAACCGCTGGACGAGCCGACCGCCAGTTGGGTGAGCAATACGCTGGATGTGCCGGTCATCGACAACTACTGGCAGACCGAATCCGGCTGGCCGATTATGGCGATTGCTcgc    >  1:1062472/1‑239 (MQ=255)

                                                                                                                                                                                                                                             cgtcgAAAAAGA‑CCGGG‑TTGGGCGGTTTTTCCCA‑GCCCCG‑CCCGCCA‑TTGGCGTTCTGAAAAAATTCCCTACCGCTGAAATCCCAAAACACGATCTCTCGTCGCTGGAAGTGCTCTATCTGGCGGGAGAACCGCTGGACGAGCCGACCGCCAGTTGGGTGAGCAATACGCTGGATGTGCCGGTCATCGACAACTACTGGCAGACCGAATCCGGCTGGCCGATTATGGCGATTGCTcgcg   <  2:774453/239‑1 (MQ=255)

                                                                                                                                                                                                                                               tcgAGAAATA‑TCAGG‑TTAGCCGGATGTTCTCA‑GCGCCG‑ACCGCCA‑TTCGCGTGCTGAAAAAATTCCCTACCGCTGAAATTCGCAAACACGATCTCTCGTCGCTGGAAGTGCTCTATCTGGCTGGAGAACCGCTGGACGAGCCGACCGCCAGTTGGGTGAGCAATACGCTGGATGTGCCGGTCATCGACAACTACTGGCAGACCGAATCCGGCTGGCCGATTATGGCGATTGCTCGCgg  >  1:1226836/1‑238 (MQ=255)

                                                                                                                                                                                                                                                 gagaAATA‑TCAGG‑TTAGCCGGATGTTCTCA‑GCGCCG‑ACCGCCA‑TTCGCGTGCTGAAAAAATTCCCTACCGCTGAAATTCGCAAACACGATCTCTCGTCGCTGGAAGTGCTCTATCTGGCTGGAGAACCGCTGGACGAGCCGACCGCCAGTTGGGTGAGCAATACGCTGGATGTGCCGGTCATCGACAACTACTGGCAGACCGAATCCGGCTGGCCGATTATGGCGATTGCTCGCgg  >  1:789635/1‑236 (MQ=255)

GACCGGCAAACCTAAAGGCGTGCAGCGTGACGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCAGCGTGTTCTTTTGCGCATCGGATATCGGCTGGGTGGTGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTATCGTTTACGAAGGATTGCCGACCTGGCC‑GGACTGCGG‑CGT‑GTGGTGGACAATCGTCGAGAAATA‑TCAGG‑TTAGCCGGATGTTCTCA‑GCGCCG‑ACCGCCA‑TTCGCGTGCTGAAAAAATTCCCTACCGCTGAAATTCGCAAACACGATCTCTCGTCGCTGGAAGTGCTCTATCTGGCTGGAGAACCGCTGGACGAGCCGACCGCCAGTTGGGTGAGCAATACGCTGGATGTGCCGGTCATCGACAACTACTGGCAGACCGAATCCGGCTGGCCGATTATGGCGATTGCTCGCGG  >  NC_000913/353434‑353907

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`