Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	3,862,674	C→A	13.3%	pseudogene (930/1617 nt)	glvC ←	pseudogene, arbutin specific enzyme IIC component of PTS;enzyme; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system, arbutin‑like IIB component; PTS system, arbutin‑like IIC component

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,862,674	0	C	A	13.3%	94.8 / 2.1	78	pseudogene (930/1617 nt)	glvC	pseudogene, arbutin specific enzyme IIC component of PTS;enzyme; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system, arbutin‑like IIB component; PTS system, arbutin‑like IIC component
Reads supporting (aligned to +/- strand):  ref base C (30/36);  new base A (3/8);  total (34/44)
Fisher's exact test for biased strand distribution p-value = 3.35e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.26e-02

TTTGGGTCAGCATCATATCCGCGTGGTTGCTGAACATCGGGATCCAGTTTTGCGGT‑AAAACCTAG‑TCAATCAGACCTCCGCCCATGTTGCCCACCACACCAAAGAGATACATTACGGTCGACATTGAGG‑CCGCCAGCACGGCGTGTACCGCAAAC‑AGCAACGGTGAAATGAACAG‑GAAGGTAAAT‑T‑CC‑AGCGGTT‑CGGTAA‑TT‑CCCA‑CCAG‑CATGGC‑GGTTA‑A‑GG‑TTGCGGGAATCAG‑CAAG‑CCCGCCAC‑TTTTA‑CCCGATTTTCCGGTGCGGCAGTGAAGTACATCGCTAAAGAAATGCCCA‑CGGCA‑CCAA‑AGA‑TTTTTGA‑GT‑TACCGTGCAGGGCAAAACCGCCTTCCGGGAACAACGATTTCAGCGGCT‑C‑GGCGCTCAAACTGAACTC‑TTGCAGATGCTGCGCCCAGTACATCTGAATGCCGCCTTCAACAGCTGCCGGACCAAAGATAAACTGTCCGTAGATGAAG  >  NC_000913/3862436‑3862905                                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                    tttGGGTCAGCATCATATCCGCGTGGTTGCTGAACATCGGGATCCAGTTTTGCGGT‑AAAACCTAG‑TCAATCAGACCTCCGCCCATGTTGCCCACCACACCAAAGAGATACATTACGGTCGACATTGAGG‑CCGCCAGCACGGCGTGTACCGCAAAC‑AGCAACGGTGAAATGAACAG‑GAAGGTAAAT‑T‑CC‑AGCGGTT‑CGGTAA‑TT‑CCCA‑CCAG‑CATGGC‑GGTTA‑A‑AG‑TTGCGGGGAACAG‑c                                                                                                                                                                                                                                                     >  1:790821/1‑239 (MQ=255)        cAGCATCATATCCGCGTGGTTGCTGAACATCGGGATCCAGTTTTGCGGT‑AAAACCTAG‑TCAATCAGACCTCCGCCCATGTTGCCCACCACACCAAAGAGATACATTACGGTCGACATTGAGG‑CCGCCAGCACGGCGTGTACCGCAAAC‑AGCAACGGTGAAATGAACAG‑GAAGGTAAAT‑T‑CC‑AGCGGTT‑CGGTAA‑TT‑CCCA‑CCAG‑CATGGC‑GGTTA‑A‑AG‑GTGCGGGAAATAG‑CAAG‑ccc                                                                                                                                                                                                                                              >  1:318034/1‑238 (MQ=255)               atatCCGCGTGGTTGCTGAACATCGGGATCCAGTTTTGCGGT‑AAAACCTAG‑TCAATCAGACCTCCGCCCATGTTGCCCACCACACCAAAGAGATACATTACGGTCGACATTGAGG‑CCGCCAGCACGGCGTGTACCGCAAAC‑AGCAACGGGGAAATGAACAG‑AAAGGGAAAT‑T‑CC‑AGAGGTT‑CGGTAA‑TT‑CCCA‑CCAG‑CATGGC‑GGTTACA‑GG‑TT‑CGGGGATCAG‑CCAA‑GCCGCCAC‑ttt                                                                                                                                                                                                                                     >  1:1412847/1‑239 (MQ=255)                     gcgTGGTTGCTGAACATCGGGATCCGGTTTTGCGGT‑AAAACCTAG‑TCAATCAGACCTCCGCCCATGTTGCCCACCACACCAAAGAGATACATTACGGTCGACATTGAGG‑CCGCCAGCACGGCGTGTACCGCAAAC‑AGCAACGGTGAAATGAACAG‑GAAGGTAAAT‑T‑CC‑AGCGGTT‑CGGTAA‑TT‑CCCA‑CCAG‑CATGGC‑GGTTT‑A‑TA‑GTGAGGGGATCCG‑CCAA‑CCCCCCAC‑CTTTT‑ccc                                                                                                                                                                                                                               >  1:457582/1‑238 (MQ=255)                     gcgTGGTTGCTGAACATCGGGATCCAGTTTTGCGGT‑AAAACCTAG‑TCAATCAGACCTCCGCCCATGTTGCCCACCACACCAGAGAGATACATTACGGTCGACATTGAGG‑CCGCCAGCACGGCGTGTACCGCAAAC‑AGCAACGGTGAAATGAACAG‑GAAGGTAAAT‑T‑CC‑AGCGGTT‑CGGTAA‑TT‑CCCA‑CCAG‑CATGGC‑GGTTA‑A‑AG‑TTGCGGGGATCAA‑CAAG‑CCCGCCAC‑TTTTT‑ccc                                                                                                                                                                                                                               >  1:289763/1‑238 (MQ=255)                     gcgTGGTTGCTGAACATCGGGATCCAGTTTTGCGGT‑AAAACCTAG‑TCAATCAGACCTCCGCCCATGTTGCCCACCACACCAAAGAGATACATTACGGTCGACATTGAGG‑CCGCCAGCACGGCGTGTACCGCAAAC‑AGCAACGGTGAAATGAACAG‑GAAGGTAAAT‑T‑CC‑AGCGGTT‑CGGTAA‑TT‑CCCA‑CCAG‑CATGGC‑GGTTT‑A‑AG‑TTGCGGGGAGCAG‑ACAA‑CCCGccccatcttccat                                                                                                                                                                                                                                >  2:750237/1‑228 (MQ=255)                     gcgTGGTTGCTGAACATCGGGATCCAGTTTTGCGGT‑AAAACCTAG‑TCAATCAGACCTCCGCCCATGTTGCCCACCACACCAAAGAGATACATTACGGTCGACATTGAGG‑CCGCCAGCACGGCGTGTACCGCAAAC‑AGCAACGGTGAAATGAACAG‑GAAGGTAAAT‑T‑CC‑AGCGGTT‑CGGTAA‑TT‑CCCA‑CCAG‑CATGGC‑GGTTA‑A‑AG‑GTGTGGGGATCAG‑CAAA‑ACCCCCCC‑TTTTT‑ccc                                                                                                                                                                                                                               >  1:181023/1‑238 (MQ=255)                     gcgTGGTTGCTGAACATCGGGATCCAGTTTTGCGGT‑AAAACCTAG‑TCAATCAGACCTCCGCCCATGTTGCCCACCACACCAAAGAGATACATTACGGTCGACATTGAGG‑CCGCCAGCACGGCGTGTACCGCAAAC‑AGCAACGGTGAAATGAACAG‑GAAGGTAAAT‑T‑CC‑AGCGGTT‑CGGTAA‑TT‑CCCA‑CCAG‑CATGGC‑GGGTT‑A‑GG‑TTGCGGGGAACAA‑CAAG‑CCCGCCCC‑tttttacc                                                                                                                                                                                                                                >  1:825566/1‑234 (MQ=255)                     gcgTGGTTGCTGAACATCGGGATCCAGTTTTGCGGT‑AAAACCTAG‑TCAATCAGACCTCCGCCCATGTTGCCCACCACACCAAAGAGATACATTACGGTCGACATTGAGG‑CCGCCAGCACGGCGTGTACCGCAAAC‑AGCAACGGTGAAATGAACAG‑GAAGGTAAAT‑T‑CC‑AGCGGGT‑CGGGAA‑TT‑CCCA‑CCAG‑AATGGC‑GGGTA‑A‑AG‑TTGAGGGGATAAGCCAAG‑CAC‑CCAC‑tttttacc                                                                                                                                                                                                                                >  1:714003/1‑234 (MQ=255)                     gcgTGGTTGCTGAACATCGGGATCCAGTTTTGCGGT‑AAAACCTAG‑TCAATCAGACCTCCGCCCATGTTGCCCACCACACCAAAGAGATACATTACGGTCGACATTGAGG‑CCGCCAGCACGGCGTGTACCGCAAAC‑AGCAACGGTGAAATGAACAG‑GAAGGGAAAT‑T‑CC‑AGCGGTT‑CGGTAA‑TT‑CCCA‑CCAG‑CATGGC‑GGTTA‑A‑AG‑GTGGGGGGATGAG‑CAAA‑CCCCCCCC‑TTTTT‑ccc                                                                                                                                                                                                                               >  1:1378929/1‑238 (MQ=255)                      cgTGGTTGCTGAACATCGGGATCCAGTTTTGCGGT‑AAAACCTAG‑TCAATCAGACCTCCGCCCATGTTGCCCACCACACCAAAGAGATACATTACGGTCGACATTGAGG‑CCGCCAGCACGGCGTGTACCGCAAAC‑AGCAACGGTGAAATGAACAG‑GAAGGTAAAT‑T‑CC‑AGCGGTT‑CGGTAA‑TT‑CCCA‑CCAG‑CATGGC‑GGTTA‑A‑GG‑GGGCGGGGATCAG‑CAAG‑CCCGCCAC‑TTTTT‑CCCgg                                                                                                                                                                                                                             >  1:903045/1‑238 (MQ=255)                          gTTGCTGAACATCGGGATCCAGTTTTGCGGT‑AAAACCTAG‑TCAATCAGACCTCCGCCCATGTTGCCCACCACACCAAAGAGATACATTACGGTCGACATTGAGG‑CCGCCAGCACGGCGTGTACCGCAAAC‑AGCAACGGTGAAATGAACAG‑GAAGGTAAAT‑T‑CC‑AGCGGTT‑CGGTAA‑TT‑CCCA‑CCAG‑CATGGC‑GGGTA‑A‑GG‑TTGGGGGGATCAG‑CAAA‑CCCCCCCC‑CTTTT‑CCCCGtttt                                                                                                                                                                                                                         >  2:1089673/1‑239 (MQ=255)                          gTTGCTGAACATCGGGATCCAGTTTTGCGGT‑AAAACCTAG‑TCAATCAGACCTCCGCCCATGTTGCCCACCACACCAAAGAGATACATTACGGTCGACATTGAGG‑CCGCCAGCACGGCGTGTACCGCAAAC‑AGCAAAGGTGAAATGAACAG‑GAAGGTAAAT‑T‑CC‑AGCGGTT‑CGGTAA‑TT‑CCCA‑CCAG‑CATGGC‑GGTTA‑A‑GG‑TGGCGGGGATTAG‑GAAG‑ACCCCCCC‑tttttcaccgtttt                                                                                                                                                                                                                          >  2:55985/1‑229 (MQ=255)                                 aaCAGCGCGACCCAGTTTTGGGGA‑AAAACTAGG‑TCAACCGGCCCCCCACCAATGTGCCCCCCCACCCAAAAGGAATACATTACGGTCGACATGGAGG‑CGGCCAGCCCGGGGTGACCCGAAAAC‑AGCAGCGGTGAAATGAACAG‑AAAGGTAAAT‑T‑CC‑GGGGGTT‑GGGTAA‑TT‑CCCA‑CCAG‑CAGGGC‑GGTTA‑G‑GG‑TGGCGGGAATCAC‑CAAG‑CCCGCCAC‑TTTTA‑CCCGATTTTCCGGTg                                                                                                                                                                                                                   <  2:1129762/238‑1 (MQ=255)                                      cGGGATCCAGTTTTGCGGT‑AAAACCTAG‑TCAATCAGACCTCCGCCCATGTTGCCCACCACACCAAAGAGATACATTACGGTCGACATTGAGG‑CCGCCAGCACGGCGTGTACCGCAAAC‑AGCAACGGTGAAATGAACAG‑GAAGGTAAAT‑T‑CC‑AGCGGTT‑CGGTAA‑TT‑CCCA‑CCAG‑CATGGC‑GGTTA‑A‑GG‑GTGTGGGGATCAG‑CAAG‑CCCCGCACTTTTTA‑CCCG‑TTTTTCGGTGCGGGAg                                                                                                                                                                                                             >  1:30464/1‑239 (MQ=255)                                             ctgTTTTG‑GGC‑AAAACCCAGTTCACTCGCACCCCGGCCCATGTCCCCCACCCCCCCAAAAGGATACATTCCGTGCGACAGGGAGG‑CCCCGAGCCGGGCGTGTACCGAAAAC‑AGAAACGGAGAAATGAAGAG‑GAATG‑AAAT‑T‑CC‑CGGGGTT‑CGGTAA‑TTCCCCA‑CCGT‑CTGGGC‑GGTTA‑G‑GG‑TTCGGGAAATCAG‑CAAC‑CCCGCCAC‑TTTTA‑CCCGATTTTCCGGTGCGGCAGTGAAGTa                                                                                                                                                                                                      <  2:802187/237‑1 (MQ=255)                                             cggTTTTGCGAA‑AAAACCAAG‑ACAAACAGACCCCCGCCCATGTCCCCCCCCCCCCCAAAGAAAACCATTCGGGTCGACATGGAGG‑CCGCCAGCAGGGCGTGTCCCGCAAAA‑AGCAGGGGTGAAAGGAAGGG‑GAA‑GTAAAT‑TCCC‑GGGGGTT‑GGGTAA‑TC‑CCCA‑CCAG‑CAGGGG‑GGTTA‑G‑GG‑TTGCGGAAATCAG‑CAAG‑CCCGCCAC‑TTTTA‑CCCGATTTTCCGGTGCGGCAGTGAAGTa                                                                                                                                                                                                      <  2:971338/237‑1 (MQ=255)                                               gTTTTGAGGTAAAAACCTGG‑ACAAACAGCCCCCCCCCCTGGTGCCCCCCCACCCAAAAAAGAAACATTACGGTCGACAGGGAGC‑CCGCAAGCGCGGGGTGTCCACCAAAC‑AGCAAGGGGAAAAGGAACAG‑GAAGGAAAAT‑C‑CC‑AGGGGTT‑CGTTAA‑TC‑CCCA‑CCGG‑CGGGGC‑GGTTA‑G‑GG‑TGGCGGGAATCAG‑AAAG‑CCCGCCAC‑TTTTA‑CCCGATTTTCCGGTGCGGCAGTGAAGTAc                                                                                                                                                                                                     <  2:500797/239‑1 (MQ=255)                                                ttttGCGGT‑AAAACCTAG‑TCAATCAGACCTCCGCCCATGTTGCCCACCACACCAAAGAGATACATTACGGTCGACATTGAGG‑CCGCCAGCACGGCGTGTACCGCAAAC‑AGCAACGGTGAAATGAACAG‑GAAGGTAAAT‑T‑CC‑AGCGGTT‑CGGTAA‑TT‑CCCA‑CCAG‑CATGGC‑GGTTA‑A‑AG‑ATGCGGGGAACAG‑CAAG‑CCCGCCAC‑CTTTT‑ACCGGTTTTTCGGGGCGGCAGGGAAGGACa                                                                                                                                                                                                    >  1:857407/1‑238 (MQ=255)                                                     cGGT‑AAAACCTAG‑TCAATCAGACCTCCGCCCATGTTGCCCACCACACCAAAGAGATACATTACGGTCGACATTGAGG‑CCGCCAGCACGGCGTGTACCGCAAAC‑AGCAACGGTGAAATGAACAG‑GAAGGTAAAT‑T‑CC‑AGCGGTT‑CGGTAA‑TT‑CCCA‑CCAG‑CATGGC‑GGTTA‑A‑GG‑GTGCGGGGATAAG‑CAAG‑CCCCCCCC‑CTTTA‑CCCGATTTTTCGGGGCGGCCGTGGAGGACATCGCTa                                                                                                                                                                                              >  2:1130446/1‑239 (MQ=255)                                                                             cccccGCCCATGTTCCCCACCACACAAAAGAAATACATTAGGGTCAAAATGGAGG‑CCGCCAGCACGGCGTGGACCGCAAAC‑AGAAAGGGGGAAATGAACAG‑GAAGTAAAAT‑T‑CA‑GGCGGTT‑CGGTAT‑TC‑CCCA‑CCAG‑CAGGGG‑GGTTA‑A‑GG‑TTGGGGGAATCAG‑CAAG‑CCCGCCAC‑TTTTA‑CCCGATTTTCCGGTGCGGCAGTGAAGTACATCGCTAAAGAAATGCCCA‑CGGCA‑CCaa                                                                                                                                                                       <  1:149081/235‑1 (MQ=255)                                                                                     tatttcccccACCACACAAAAGAGATACATTACGGTCGATATGGAGC‑CGGCCAGCACGGCGTGTACCGCAAAC‑AGAAGAGGTGAAATAAACAG‑GAAGGAAATT‑T‑CC‑AGCGTTT‑CGTTAA‑TC‑CCCC‑CCAG‑CAGGGG‑GGTTA‑A‑GG‑TGGGGGGAACCAC‑AAAG‑CCCGCCAC‑TTTTA‑CCCGATTTTCCGGTGCGGCAGTGAAGTACATCGCTAAAGAAATGCCCA‑CGGCA‑CCAA‑AGA‑TTTTTg                                                                                                                                                            <  2:1412847/232‑1 (MQ=255)                                                                                               cccacaccaAAGAGATACATTGCGGCCGACATTGAGG‑CCGCCAGCACGGCGGGTACCGCAAAC‑AGCAACGGTGAAATGAAAAG‑GAAGTAAAAT‑T‑CC‑AGCGGTT‑CGGTAA‑TC‑CCCA‑CCAG‑CAGGGC‑GGTTA‑G‑GG‑TTGGGGGAATCAG‑CAAG‑CCCGCCAC‑TTTTA‑CCCGATTTTCCGGTGCGGCAGTGAAGTACATCGCTAAAGAAATGCCCA‑CGGCA‑CCAA‑AGA‑TTTTTGA‑GT‑TACCGTg                                                                                                                                                <  1:503987/238‑1 (MQ=255)                                                                                               cccacaccaAAGAGACACATCACGGTCGACAGGGAGG‑CCGCGAGCAGGGCGGGACCCACAAAC‑GGCAAGGGGAAAATAAAGAG‑GAAGGAAATT‑C‑CC‑AGCGGTT‑GGGT‑A‑TT‑CCCACCCAG‑CAGGGG‑GGTTA‑G‑GG‑TGGGGGGAATCAG‑AAAG‑CCCGCCAC‑TTTTA‑CCCGATTTTCCGGTGCGGCAGTGAAGTACATCGCTAAAGAAATGCCCA‑CGGCA‑CCAA‑AGA‑TTTTTGA‑GT‑TACCGTg                                                                                                                                                <  2:599045/238‑1 (MQ=255)                                                                                               accacaacaAAGATATACATTGCGGGCGACATGGAGG‑CCGCCAGCACGGCGTGCACCCAAAAC‑AGCAACGGTAAAAAGAACGG‑GAAGGTAAAT‑T‑CC‑GGCGGTT‑CGGTAT‑TC‑CCCC‑CCAG‑CAGGGC‑GTTTA‑A‑GG‑TTGCGGGAATCAG‑CAAG‑CCCGCCAC‑TTTTA‑CCCGATTTTCCGGTGCGGCAGTGAAGTACATCGCTAAAGAAATGCCCA‑CGGCA‑CCAA‑AGA‑TTTTTGA‑GT‑TACCGTg                                                                                                                                                <  2:209102/239‑1 (MQ=255)                                                                                                      caAAGAGATACATTACGGTCGACATTGAGG‑CCGCCAGCACGGCGTGTACCGCAAAC‑AGCAACGGTGAAATGAACAG‑GAAGGTAAAT‑T‑CC‑AGCGGTT‑CGGTAA‑TT‑CCCA‑CCAG‑CATGGC‑GGTTATA‑GG‑TGGGGGGAA‑CAG‑CAAA‑CCACCCCC‑TTTTA‑TCCCCATTTCCGGTGGGGCAGGGAAGGACATCCCCAAAAAAAATACCA‑CCGCA‑CCAA‑AAA‑TTTTTTA‑GTATACCgggtgggcg                                                                                                                                          >  2:855977/1‑231 (MQ=255)                                                                                                              tACATTACGGTCGACATTGAGG‑CCGCCAGCACGGCGTGTACCGCAAAC‑AGCAACGGTGAAATGAACAG‑GAAGGTAAAT‑T‑CC‑AGCGGTT‑CGGTAA‑TT‑CCCA‑CCAG‑CATGGC‑GGTTA‑A‑GG‑TTGGGGGAATCAG‑CAAG‑CCCGCCCC‑TTTTA‑ACCGATTTTCCGGTGCGGCAGGGGAGTAAAACGATAAAAAAAAGCCCA‑CCGCC‑CCAACAAG‑TTTTTTA‑G‑‑TACCATGCCGGGCAAAACCGc                                                                                                                                  >  2:363447/1‑238 (MQ=255)                                                                                                                   tACGGGCGACAATGGGGCCCGCAGGCACGGGGGGTACC‑CAAACAAGAACCGGAGAAATGAACAG‑GAAGGTAAAT‑T‑CA‑AGCTGTT‑CGTTAA‑TC‑CCCC‑CCAG‑CAGGGC‑GGTTA‑G‑GG‑TGGCGGAAACCAG‑CAAG‑CCCGCCAC‑TTTTA‑CCCGATTTTCCGGTGCGGCAGTGAAGTACATCGCTAAAGAAATGCCCA‑CGGCA‑CCAA‑AGA‑TTTTTGA‑GT‑TACCGTGCAGGGCAAAACCGCCTTcc                                                                                                                             <  2:691442/239‑1 (MQ=255)                                                                                                                     gggTCGCAATTGGGC‑CCGCCAGCAGGGCGTGGACCGCAAAC‑AGAAACGGAGAAAAGAACAG‑GAGGCTAATG‑T‑CG‑GGCGGGT‑AGATAA‑GC‑CCCA‑CCGG‑CAGGGC‑GGTGA‑C‑GT‑TGGGGGCAATGAG‑CAAG‑CCCGCCAC‑TTTTA‑CCCGATTTTCCGGTGCGGCAGTGAAGTACATCGCTAAAGAAATGCCCA‑CGGCA‑CCAA‑AGA‑TTTTTGA‑GT‑TACCGTGCAGGGCAAAACCGCCTTCCggg                                                                                                                          <  2:1378929/238‑1 (MQ=255)                                                                                                                     cGGTCGACATTGAGG‑CCGCCAGCACGGCGTGTACCGCAAAC‑AGCAACGGTGAAATGAACAG‑GAAGGTAAAT‑T‑CC‑AGCGGTT‑CGGTAA‑TT‑CCCA‑CCAG‑CATGGC‑GGTTA‑A‑GG‑GTTCGGGGGTCAG‑ACAG‑CCCCCCCC‑CTTTACCCCGTTTTTCCGG‑GCGGCCGGGGAGGAAATCCCGCAAAAAAATCCCA‑CCGCACCCAA‑ACA‑TTTTTTG‑GT‑TCCGTTGCGGGGC‑AAACCGCCCTCCcgg                                                                                                                          >  2:837355/1‑239 (MQ=255)                                                                                                                     cGGTCGACATTGAGG‑CCGCCAGCACGGCGTGTACCGCAAAC‑AGCAACGGTGAAATGAACAG‑GAAGGTAAAT‑T‑CC‑AGCGGTT‑CGGTAA‑TT‑CCCA‑CCAG‑CATGGC‑GGGTA‑A‑GG‑TTTCGGGGATCAG‑CAAA‑GCCGCCAC‑TTTTA‑CCCGATTTTTCCGTGCGGCAGTGAAAGAAAACCCCAAAAAAAAGCCCC‑CGGCA‑CCAA‑AGA‑ATTTTTA‑GT‑TAACGTGCAGGGCAAAAACCCCTTCCcgg                                                                                                                          >  1:1165811/1‑239 (MQ=255)                                                                                                                     cGGTCGACATTGAGG‑CCGCCAGCACGGCGTGTACCGCAAAC‑AGCAACGGTGAAATGAACAG‑GAAGGTAAAT‑T‑CC‑AGCGGTT‑CGGTAA‑TT‑CCCA‑CCAG‑CATGGC‑GGGTA‑A‑AG‑TTTCGGGAAACAG‑CCAA‑ACCGCCAC‑TTTTT‑CCCCGTTTTCCCGTGGGGCCGGGAAGTACACCGCTAAAAAAATGCCCA‑CCGGC‑CCAA‑AGA‑ATTTTTA‑GT‑TTACGTGCAGGGGAAAAACCCCtttccgg                                                                                                                          >  1:560183/1‑234 (MQ=255)                                                                                                                        tCGACATTGAGG‑CCGCCAGCACGGCGTGGACCGCAAAC‑AGCAACGGCGAAATGAACAG‑GAAGGTAACT‑T‑CC‑AGCGGTT‑CGGTAA‑TT‑CCCA‑CCAG‑CATGGCGGGTTA‑G‑GC‑TTGC‑GGAACCAG‑CAAG‑CCCGCCCC‑TTTTT‑TCCCCTTTTTCCATGGGGGAGTGAAGTGCAACGCTAAACAAATGCCCC‑CCGCA‑CCCA‑AGA‑TTTTTTAGGT‑TCCGGTGACGGGC‑ACACCGCCCTCCggggcc                                                                                                                       >  2:990244/1‑236 (MQ=255)                                                                                                                          gACATTGAGG‑CCGCCAGCACGGCGTGTACCGCAAAC‑AGCAACGGTGAAATGAACAG‑GAAGGTAAAT‑T‑CC‑AGCGGTT‑CGGTAA‑TT‑CCCA‑CCAG‑CATGGC‑GGATA‑A‑AG‑GTGCGGGAAACAA‑CCAA‑ACCCCCCC‑CTTTA‑CCCCCTTTTTCGGGGGGGGAGGGAAGGACAACGCCTAAAAAATTCCCACCGGCC‑CCAA‑AAA‑TTTTTAA‑GT‑AACCACCAGGGGAAAAACCACCTCCCGGGacaa                                                                                                                      >  2:325539/1‑236 (MQ=255)                                                                                                                           aCATTGAGG‑CCGCCAGCACGGCGTGTACCGCAAAC‑AGCAACGGTGAAATGAACAG‑GAAGGTAAAT‑T‑CC‑AGCGGTT‑CGGTAA‑TT‑CCCA‑CCAG‑CATGGC‑GGTTA‑A‑GG‑TTGCGGGGATCAG‑CAAG‑CCCCCCAC‑CTTTA‑CCCCATTTTTCGGTGCGGGCGTGGAAGACATCGCCAAAGAAAAACCCC‑CCGCC‑CCAC‑AGACTTTTTGA‑‑T‑TACCGTGCAGGGCCAAACCGCCCTCCGCGaacaaa                                                                                                                    >  2:364358/1‑238 (MQ=255)                                                                                                                                 aGG‑CCGCCAGCACGGCGTGTACCGCAAAC‑AGCAACGGTGAAATGAACAG‑GAAGGTAAAT‑T‑CC‑AGCGGTT‑CGGTAA‑TT‑CCCA‑CCAG‑CATGGC‑GGGTA‑A‑AG‑GTGCGGGGAACAG‑CAAGACCCGCCCC‑TTTTA‑CCC‑AGTTTCCGGTGCGGCCGGGGAGGAAAAAGCCGAAACAAAGACCA‑CGGCC‑CCCA‑AGA‑ATTTTGT‑GT‑TACCGTGCCGGGCAAAACACCCCTCCCGGgacaaccgattc                                                                                                              >  2:263677/1‑233 (MQ=255)                                                                                                                                                       ccacAAAC‑AGAAGCGGGAAAATGAACAG‑GAAGGTAATC‑T‑CC‑AGCGTTT‑CGTTAA‑TC‑CCCA‑CCAG‑CGTGGC‑GGTTA‑A‑GG‑TGGGGGAAATCAG‑CAAG‑CCCGCCAC‑TTTTA‑CCCGATTTTCCGGTGCGGCAGTGAAGTACATCGCTAAAGAAATGCCCA‑CGGCA‑CCAA‑AGA‑TTTTTGA‑GT‑TACCGTGCAGGGCAAAACCGCCTTCCGGGAACAACGATTTCAGCGGCT‑C‑GGCGCTCAAACTg                                                                                       <  2:519041/236‑1 (MQ=255)                                                                                                                                                         aaaacc‑AGCAACGTGAAAATAAACAG‑GAA‑GTAAAT‑T‑CCGAGCGTTG‑TGGTAA‑CT‑CCCC‑CCAG‑CAGGGC‑GGTTA‑A‑GG‑TGGCGGGAACCAG‑CAAG‑CCCGCCAC‑TTTTA‑CCCGATTTTCCGGTGCGGCAGTGAAGTACATCGCTAAAGAAATGCCCA‑CGGCA‑CCAA‑AGA‑TTTTTGA‑GT‑TACCGTGCAGGGCAAAACCGCCTTCCGGGAACAACGATTTCAGCGGCT‑C‑GGCGCTCAAACTGaa                                                                                     <  2:1201108/234‑1 (MQ=255)                                                                                                                                                          gcaacacaacaCGGTGAAATGAAAAG‑AGAGG‑AAAT‑T‑CC‑CCGGGTTACAGTAA‑CC‑CCCA‑CCAA‑AAGGGC‑GTTTA‑A‑GG‑TGGAGGGAAACAG‑CAAC‑ACCGCCAC‑TTTTA‑CCCGATTTTCCGGTGCGGCAGTGAAGTACATCGCTAAAGAAATGCCCA‑CGGCA‑CCAA‑AGA‑TTTTTGA‑GT‑TACCGTGCAGGGCAAAACCGCCTTCCGGGAACAACGATTTCAGCGGCT‑C‑GGCGCTCAAACTGaa                                                                                     <  1:702820/229‑1 (MQ=255)                                                                                                                                                          cAAAC‑AGCAACGGTGAAATGAACAG‑GAAGGTAAAT‑T‑CC‑AGCGGTT‑CGGTAA‑TT‑CCCA‑CCAG‑CATGGC‑GGTTA‑A‑GG‑TTGCGGGAATCAG‑CAAG‑CCCGCCAC‑TTTTA‑CCCGATTTTCCGGTGCGGCAGTGAAGGACAACGCTAAAGAAATGCCCA‑CGGCC‑CCCA‑AGA‑TTTTTTA‑GG‑TATCGTGCAGGGCAAAACCGCCTTCCGGGGACAACGATTTCAGCGGCT‑C‑GGCGCTCAAAATGAAc                                                                                    >  1:504044/1‑239 (MQ=255)                                                                                                                                                                aGCAACGGTGAAATGAACAG‑GAAGGTAAAT‑T‑CC‑AGCGGTT‑CGGTAA‑TT‑CCCA‑CCAG‑CATGGC‑GGTTA‑A‑GG‑TTTCGGGAAACAG‑CAAG‑CCCGCCCC‑CTTTT‑CCCCATTTTCCGGGGGGGCAGGGAAGGACCACGCTAAAAAAAATCCCA‑CCGCA‑CCCA‑AGA‑TTTTTTA‑GT‑TTACGTGCCGGGCAAAACCGCCTTCCGGGAACAACGATTTCAGCCGCTCC‑GGGGCCCAAAATGaatcct                                                                                 >  2:950982/1‑234 (MQ=255)                                                                                                                                                                   aaCGGGAAAATGAACAG‑GAAGGTAAAT‑T‑CC‑GGCGGTC‑CGGTAA‑TT‑CCCC‑CCAG‑CATGGC‑GGTTA‑G‑GG‑TTGCGGAAATCAG‑CAAG‑CCCGCCAC‑TTTTA‑CCCGATTTTCCGGTGCGGCAGTGAAGTACATCGCTAAAGAAATGCCCA‑CGGCA‑CCAA‑AGA‑TTTTTGA‑GT‑TACCGTGCAGGGCAAAACCGCCTTCCGGGAACAACGATTTCAGCGGCT‑C‑GGCGCTCAAACTGAACTC‑TTGCAg                                                                           <  1:1071264/239‑1 (MQ=255)                                                                                                                                                                        tGAAATGAACAG‑GAAGGTAAAT‑T‑CC‑AGCGGTT‑CGGTAA‑TT‑CCCA‑CCAG‑CATGGC‑GGTTA‑A‑GG‑TTGCGGGAATCAG‑CAAG‑CCCGCCCC‑CTTTA‑CCCCATTTTTCGGTGCGGCAGTGAAGTACATCGCCAAAAAAATACCCA‑CGGCC‑CCAA‑AGA‑TTTTTTA‑GT‑TACCGTGCAGGGCAAAAACGCCCTCCGGGAAAAACGATTTTCGCGGCC‑C‑GGGCCCCAAACTGGACCC‑TTTCAGAt                                                                         >  2:1345907/1‑236 (MQ=255)                                                                                                                                                                         aaaaagaaaag‑AATGGTAAAT‑T‑CC‑AGGGGTT‑CGGTAA‑TC‑CCCA‑CCAG‑AAGGGC‑GTTAA‑G‑GG‑TTGCGGGAATCAG‑AAAA‑CCCGCCAC‑TTTTA‑CCCGATTTTCCGGTGCGGCAGTGAAGTACATCGCTAAAGAAATGCCCA‑CGGCA‑CCAA‑AGA‑TTTTTGA‑GT‑TACCGTGCAGGGCAAAACCGCCTTCCGGGAACAACGATTTCAGCGGCT‑C‑GGCGCTCAAACTGAACTC‑TTGCAGAtgct                                                                      <  1:148216/237‑1 (MQ=255)                                                                                                                                                                          aaaTGAACAGTGAAGTTAATT‑T‑CT‑GGGGTTC‑CGGTAT‑TC‑CCGA‑CGAG‑CAGGGC‑GGTTA‑G‑GG‑TGGGGGAAAGCAG‑AAAG‑CCCGCCAC‑TTTTA‑CCCGATTTTCCGGTGCGGCAGTGAAGTACATCGCTAAAGAAATGCCCA‑CGGCA‑CCAA‑AGA‑TTTTTGA‑GT‑TACCGTGCAGGGCAAAACCGCCTTCCGGGAACAACGATTTCAGCGGCT‑C‑GGCGCTCAAACTGAACTC‑TTGCAGAtgct                                                                      <  2:815081/238‑1 (MQ=255)                                                                                                                                                                          aaaTGAACAG‑GAAGGTAAAT‑T‑CC‑AGCGGTT‑CGGTAA‑TT‑CCCA‑CCAG‑CATGGC‑GGTTA‑A‑GG‑GTGCGGGAATCAG‑CAAG‑CCCGCCAC‑CTTTA‑CCCGATTTTTCGGTGCGGCAGTGAAGTAAATCGCTAAAGAAATGCCCC‑CGGCA‑CCAA‑AGA‑TTTTTTA‑GT‑TACCGTGCCGGGCAAAACCGCCTTCCCGGAACAACGATTTCAGCGGCT‑CGGGGGCCCAAATTG‑ACTC‑TTTCAGAtggtg                                                                     >  2:431485/1‑238 (MQ=255)                                                                                                                                                                           aaTGAGCAG‑GTAGGAAAAT‑C‑CC‑AGCAGTT‑CGGTAA‑GC‑CCCC‑CCGT‑CAGGGC‑GTTTA‑A‑GG‑TTGCGGAAACCAG‑AAGG‑CCCGCCAC‑TTTTA‑CCCGATTTTCCGGTGCGGCAGTGAAGTACATCGCTAAAGAAATGCCCA‑CGGCA‑CCAA‑AGA‑TTTTTGA‑GT‑TACCGTGCAGGGCAAAACCGCCTTCCGGGAACAACGATTTCAGCGGCT‑C‑GGCGCTCAAACTGAACTC‑TTGCAGATGCTgcg                                                                   <  1:344983/239‑1 (MQ=255)                                                                                                                                                                           aaTGAACAG‑GAGGGTAATT‑C‑CG‑GGGGGGT‑CGTTAA‑TC‑CCCC‑CCCG‑CGTGGG‑GGTTA‑G‑GG‑TTGCGGGAATCAG‑CAAC‑CCCGCCAC‑TTTTA‑CCCGATTTTCCGGTGCGGCAGTGAAGTACATCGCTAAAGAAATGCCCA‑CGGCA‑CCAA‑AGA‑TTTTTGA‑GT‑TACCGTGCAGGGCAAAACCGCCTTCCGGGAACAACGATTTCAGCGGCT‑C‑GGCGCTCAAACTGAACTC‑TTGCAGATGCTgcg                                                                   <  2:614651/239‑1 (MQ=255)                                                                                                                                                                           aaTGAACAG‑GAGGAAAAAT‑T‑CC‑AGCGGTT‑CGTTAA‑TT‑CCCA‑CCAG‑CAGGGC‑GGTTA‑A‑GG‑TGGCGGAAATCAG‑CAAC‑CCCGCCAC‑TTTTA‑CCCGATTTTCCGGTGCGGCAGTGAAGTACATCGCTAAAGAAATGCCCA‑CGGCA‑CCAA‑AGA‑TTTTTGA‑GT‑TACCGTGCAGGGCAAAACCGCCTTCCGGGAACAACGATTTCAGCGGCT‑C‑GGCGCTCAAACTGAACTC‑TTGCAGATGCTgcg                                                                   <  1:1057897/239‑1 (MQ=255)                                                                                                                                                                             tgacaaG‑GAAGGTAAAT‑G‑CC‑CGCGGTT‑‑GGTAATTT‑CCCC‑CCAG‑AGTGGC‑GGTTA‑G‑GG‑TGGAGGAAATCAG‑CAAC‑CCCGCCAC‑TTTTA‑CCCGATTTTCCGGTGCGGCAGTGAAGTACATCGCTAAAGAAATGCCCA‑CGGCA‑CCAA‑AGA‑TTTTTGA‑GT‑TACCGTGCAGGGCAAAACCGCCTTCCGGGAACAACGATTTCAGCGGCT‑C‑GGCGCTCAAACTGAACTC‑TTGCAGATGCTGCGcc                                                                 <  1:277568/234‑1 (MQ=255)                                                                                                                                                                             tGAACAG‑GAAGTAAATT‑T‑CC‑AGCGGTT‑CGTTAA‑TC‑CCCC‑CCAG‑CATGGC‑GGTTA‑A‑GT‑GGGCGGGAATCAG‑CAAG‑CCCGCCAC‑TTTTA‑CCCGATTTTCCGGTGCGGCAGTGAAGTACATCGCTAAAGAAATGCCCA‑CGGCA‑CCAA‑AGA‑TTTTTGA‑GT‑TACCGTGCAGGGCAAAACCGCCTTCCGGGAACAACGATTTCAGCGGCT‑C‑GGCGCTCAAACTGAACTC‑TTGCAGATGCTGCGcc                                                                 <  1:855977/239‑1 (MQ=255)                                                                                                                                                                             tGAACAG‑GAAGATAAAT‑C‑CC‑AGCGGTT‑CGGTAT‑TC‑CCCC‑CCAG‑GTGGGC‑GTTTA‑G‑GG‑TTGCGGGAACCAG‑CAGC‑CCCGCCAC‑TTTTA‑CCCGATTTTCCGGTGCGGCAGTGAAGTACATCGCTAAAGAAATGCCCA‑CGGCA‑CCAA‑AGA‑TTTTTGA‑GT‑TACCGTGCAGGGCAAAACCGCCTTCCGGGAACAACGATTTCAGCGGCT‑C‑GGCGCTCAAACTGAACTC‑TTGCAGATGCTGCGcc                                                                 <  1:776243/239‑1 (MQ=255)                                                                                                                                                                               aaagg‑gAAGAAAAAT‑C‑CC‑AGAGGTG‑CGTGAA‑TC‑CCCA‑CAGG‑CTGGGT‑GATAA‑A‑GG‑TTGGGGGAATCAC‑CAAC‑CCCGCCAC‑TTTTA‑CCCGATTTTCCGGTGCGGCAGTGAAGTACATCGCTAAAGAAATGCCCA‑CGGCA‑CCAA‑AGA‑TTTTTGA‑GT‑TACCGTGCAGGGCAAAACCGCCTTCCGGGAACAACGATTTCAGCGGCT‑C‑GGCGCTCAAACTGAACTC‑TTGCAGATGCTGCGcc                                                                 <  2:638797/233‑1 (MQ=255)                                                                                                                                                                                aagg‑gAAGTAAATT‑T‑CC‑ACGGTTT‑CGTTAC‑TC‑CCCA‑CCAG‑TATGGT‑GGTTA‑A‑GG‑TTGGGGGAATCAG‑AAAG‑CCCGCCAC‑TTTTA‑CCCGATTTTCCGGTGCGGCAGTGAAGTACATCGCTAAAGAAATGCCCA‑CGGCA‑CCAA‑AGA‑TTTTTGA‑GT‑TACCGTGCAGGGCAAAACCGCCTTCCGGGAACAACGATTTCAGCGGCT‑C‑GGCGCTCAAACTGAACTC‑TTGCAGATGCTGCGCCCAg                                                              <  1:1403231/236‑1 (MQ=255)                                                                                                                                                                                aCAG‑GAA‑GTAAATGT‑CC‑CGCGGTT‑GGGTAA‑TT‑CCCA‑CCCG‑CATGGC‑GTTTA‑G‑GG‑TTGCGGAAATAAG‑CAAG‑CCCGCCAC‑TTTTA‑CCCGATTTTCCGGTGCGGCAGTGAAGTACATCGCTAAAGAAATGCCCA‑CGGCA‑CCAA‑AGA‑TTTTTGA‑GT‑TACCGTGCAGGGCAAAACCGCCTTCCGGGAACAACGATTTCAGCGGCT‑C‑GGCGCTCAAACTGAACTC‑TTGCAGATGCTGCGCCCAg                                                              <  2:871218/239‑1 (MQ=255)                                                                                                                                                                                  aG‑GAAGGTAAAT‑T‑CG‑GGCGGTT‑CGGTAA‑TT‑CCCA‑CCAG‑CGTGGC‑GTTGA‑A‑GG‑TTGCGGAAAGCAG‑CAAG‑CCCGCCAC‑TTTTA‑CCCGATTTTCCGGTGCGGCAGTGAAGTACATCGCTAAAGAAATGCCCA‑CGGCA‑CCAA‑AGA‑TTTTTGA‑GT‑TACCGTGCAGGGCAAAACCGCCTTCCGGGAACAACGATTTCAGCGGCT‑C‑GGCGCTCAAACTGAACTC‑TTGCAGATGCTGCGcc                                                                 <  1:920924/234‑1 (MQ=255)                                                                                                                                                                                      aaGGT‑AAT‑T‑CC‑CGGGGTT‑CGGGTA‑TC‑CCCC‑CCGGACAGGGT‑GGTTA‑G‑GG‑TTGGGGAAACAAC‑CACG‑CCCGCCAC‑TTTTA‑CCCGATTTTCCGGTGCGGCAGTGAAGTACATCGCTAAAGAAATGCCCA‑CGGCA‑CCAA‑AGA‑TTTTTGA‑GT‑TACCGTGCAGGGCAAAACCGCCTTCCGGGAACAACGATTTCAGCGGCT‑C‑GGCGCTCAAACTGAACTC‑TTGCAGATGCTGCGCCCAGTACAt                                                         <  2:976403/239‑1 (MQ=255)                                                                                                                                                                                             aT‑T‑CC‑CGCGGTG‑CTGTAA‑TC‑CCCC‑CCAG‑CAT‑GC‑GGTTT‑A‑GGTTTGCGGGAACCAG‑CAAC‑CCCGCCAC‑TTTTA‑CCCGATTTTCCGGTGCGGCAGTGAAGTACATCGCTAAAGAAATGCCCA‑CGGCA‑CCAA‑AGA‑TTTTTGA‑GT‑TACCGTGCAGGGCAAAACCGCCTTCCGGGAACAACGATTTCAGCGGCT‑C‑GGCGCTCAAACTGAACTC‑TTGCAGATGCTGCGCCCAGTACATCTGAATg                                                  <  1:1345084/239‑1 (MQ=255)                                                                                                                                                                                               ttccc‑AGCGGTT‑CGTTAA‑TC‑CCCC‑CAAC‑GAGGGC‑GGTTA‑A‑GG‑TGGGGGGAATCAG‑CAAG‑CCCGCCAC‑TTTTA‑CCCGATTTTCCGGTGCGGCAGTGAAGTACATCGCTAAAGAAATGCCCA‑CGGCA‑CCAA‑AGA‑TTTTTGA‑GT‑TACCGTGCAGGGCAAAACCGCCTTCCGGGAACAACGATTTCAGCGGCT‑C‑GGCGCTCAAACTGAACTC‑TTGCAGATGCTGCGCCCAGTACATCTGAATg                                                  <  1:1346214/236‑1 (MQ=255)                                                                                                                                                                                                atcccggCGGTT‑CGGTAA‑TC‑CCCA‑CCAG‑GAGGGC‑GTTTA‑G‑GG‑TGGGGGGAATCAG‑CAAC‑CCCGCCAC‑TTTTA‑CCCGATTTTCCGGTGCGGCAGTGAAGTACATCGCTAAAGAAATGCCCA‑CGGCA‑CCAA‑AGA‑TTTTTGA‑GT‑TACCGTGCAGGGCAAAACCGCCTTCCGGGAACAACGATTTCAGCGGCT‑C‑GGCGCTCAAACTGAACTC‑TTGCAGATGCTGCGCCCAGTACATCTGAATg                                                  <  1:950982/233‑1 (MQ=255)                                                                                                                                                                                                atccaaGCGGTT‑CGGTAA‑TC‑CCCA‑CCAG‑CGTGGC‑GGTTA‑A‑GG‑TGGCGGGAATCAG‑CAAG‑CCCGCCAC‑TTTTA‑CCCGATTTTCCGGTGCGGCAGTGAAGTACATCGCTAAAGAAATGCCCA‑CGGCA‑CCAA‑AGA‑TTTTTGA‑GT‑TACCGTGCAGGGCAAAACCGCCTTCCGGGAACAACGATTTCAGCGGCT‑C‑GGCGCTCAAACTGAACTC‑TTGCAGATGCTGCGCCCAGTACATCTGAATg                                                  <  1:1130446/234‑1 (MQ=255)                                                                                                                                                                                                aggcgaGCGGGT‑CGTTAA‑TC‑CCCA‑CCAG‑CA‑GGG‑GGTTA‑ATGG‑TGGCGGAAAACAG‑CACG‑CCCGCCAC‑TTTTA‑CCCGATTTTCCGGTGCGGCAGTGAAGTACATCGCTAAAGAAATGCCCA‑CGGCA‑CCAA‑AGA‑TTTTTGA‑GT‑TACCGTGCAGGGCAAAACCGCCTTCCGGGAACAACGATTTCAGCGGCT‑C‑GGCGCTCAAACTGAACTC‑TTGCAGATGCTGCGCCCAGTACATCTGAATg                                                  <  2:489542/234‑1 (MQ=255)                                                                                                                                                                                                  cccgggggTT‑GGGTAC‑CT‑CCCA‑CACG‑CATGGT‑GGTTG‑T‑GG‑TTGCGGGAACAAC‑CAAC‑CCCGCCAC‑TTTTA‑CCCGATTTTCCGGTGCGGCAGTGAAGTACATCGCTAAAGAAATGCCCA‑CGGCA‑CCAA‑AGA‑TTTTTGA‑GT‑TACCGTGCAGGGCAAAACCGCCTTCCGGGAACAACGATTTCAGCGGCT‑C‑GGCGCTCAAACTGAACTC‑TTGCAGATGCTGCGCCCAGTACATCTGAATgcc                                                <  2:1360069/233‑1 (MQ=255)                                                                                                                                                                                                  cc‑AGCGGTG‑CGGTAA‑CT‑CCCC‑CCAT‑CGTGGC‑GGTTA‑G‑GG‑TGGCGGGAATCAG‑AAAG‑CCCGCCAC‑TTTTA‑CCCGATTTTCCGGTGCGGCAGTGAAGTACATCGCTAAAGAAATGCCCA‑CGGCA‑CCAA‑AGA‑TTTTTGA‑GT‑TACCGTGCAGGGCAAAACCGCCTTCCGGGAACAACGATTTCAGCGGCT‑C‑GGCGCTCAAACTGAACTC‑TTGCAGATGCTGCGCCCAGTACATCTGAATgccg                                               <  1:596998/239‑1 (MQ=255)                                                                                                                                                                                                                     t‑CCCA‑CCAG‑CATGGC‑GGTTA‑A‑GG‑TTGCGGGGATCAG‑CAAG‑CCCCCCCC‑CTTTA‑CCCCATTTTTCGGTGCGGCAGGGAAGTAAATCGCTAAAGAAAAGCCCC‑CGGCA‑CCAA‑AGA‑TTTTTTT‑GT‑TACCGTGCCGGGCAAAACCGCCTTCCGGGAACAACGATTTTAGCGGCT‑C‑CGCGCTCCAAACGAACCCTTTTCGCAGGTGGCGCCC‑GTACAATCGAAGTCCGCCTTCAACAGccgccg                               >  2:994221/1‑239 (MQ=255)                                                                                                                                                                                                                         ca‑ccaG‑TGTGCC‑GGTTA‑A‑GG‑GCGCGGGAGTCAG‑CAAT‑CCCGCCAC‑TTTTA‑CCCGATTTTCCGGTGCTGCAGTTAAGTACATCGCTAAAGAAATGCTCA‑CGGCA‑CCAT‑AGA‑TTTTTGA‑GT‑TACCGTGCAGGGCAAACCCGCCCTCCGGGAACAACGATTTCAGCTGCT‑C‑GGCGCTCAAACTGAACTC‑TTGCAGATGCTGCGCCTAGTCCATCTGAATGCCGCCTTCAACAGCTGCCGGag                            <  2:848869/239‑2 (MQ=255)                                                                                                                                                                                                                          ccccaG‑CATGGC‑GGTTA‑G‑GG‑TTGCGGGGAGCAG‑CAAG‑CCCGCCAC‑TTTTA‑CCCGATTTTCCGGTGCGGCAGTGAAGTACATCGCTAAAGAAATGCCCA‑CGGCA‑CCAA‑AGA‑TTTTTGA‑GT‑TACCGTGCAGGGCAAAACCGCCTTCCGGGAACAACGATTTCAGCGGCT‑C‑GGCGCTCAAACTGAACTC‑TTGCAGATGCTGCGCCCAGTACATCTGAATGCCGCCTTCAACAGCTGCCGGAc                            <  1:837355/237‑1 (MQ=255)                                                                                                                                                                                                                          ccacaG‑CGTGGC‑GGTTA‑G‑GG‑TGGGGGAAACCAC‑CAAC‑CCCGCCAC‑TTTTA‑CCCGATTTTCCGGTGCGGCAGTGAAGTACATCGCTAAAGAAATGCCCA‑CGGCA‑CCAA‑AGA‑TTTTTGA‑GT‑TACCGTGCAGGGCAAAACCGCCTTCCGGGAACAACGATTTCAGCGGCT‑C‑GGCGCTCAAACTGAACTC‑TTGCAGATGCTGCGCCCAGTACATCTGAATGCCGCCTTCAACAGCTGCCGGAc                            <  2:1264615/236‑1 (MQ=255)                                                                                                                                                                                                                          a‑ccaG‑CATGGC‑GGTTA‑A‑GG‑GTGCGGGAATCAG‑CAAG‑CCCGCCAC‑TTTTA‑CCCGATTTTCCGGTGCGGCAGTGAAGAACATCGCTAAAGAAAAGCCCA‑CGGCA‑CCAA‑AGA‑TTTTTGA‑GT‑TACCGTGCAGGGCAAAACCGCCTTCCGGGAAAAACGATTTCAGCGGCT‑C‑GGCGCTCAAACTGAACTC‑TTGCAGATGCTGCGCCCAGTACATCTGAATGCCGCCCTCAACAGCTGcccggac                           >  1:321188/1‑234 (MQ=255)                                                                                                                                                                                                                            ccaG‑CAGGGC‑GGTTA‑A‑GG‑TTGCGGGAACAAG‑CAAG‑CCCGCCAC‑TTTTA‑CCCGATTTTCCGGTGCGGCAGTGAAGTACATCGCTAAAGAAATGCCCA‑CGGCA‑CCAA‑AGA‑TTTTTGA‑GT‑TACCGTGCAGGGCAAAACCGCCTTCCGGGAACAACGATTTCAGCGGCT‑C‑GGCGCTCAAACTGAACTC‑TTGCAGATGCTGCGCCCAGTACATCTGAATGCCGCCTTCAACAGCTGCCGGACCa                          <  2:321188/239‑1 (MQ=255)                                                                                                                                                                                                                                      c‑GGTTA‑A‑GG‑GGGCGGAAAACAG‑CAAG‑CCCGCCAC‑TTTTA‑CCCGATTTTCCGGTGCGGCAGTGAAGTACATCGCTAAAGAAATGCCCA‑CGGCA‑CCAA‑AGA‑TTTTTGA‑GT‑TACCGTGCAGGGCAAAACCGCCTTCCGGGAACAACGATTTCAGCGGCT‑C‑GGCGCTCAAACTGAACTC‑TTGCAGATGCTGCGCCCAGTACATCTGAATGCCGCCTTCAACAGCTGCCGGACCAAAGATaaa                  <  2:481073/238‑1 (MQ=255)                                                                                                                                                                                                                                      c‑GGTAA‑G‑GG‑TTGCGGGAATCAG‑CAAG‑CCCGCCAC‑TTTTA‑CCCGATTTTCCGGTGCGGCAGTGAAGTACATCGCTAAAGAAATGCCCA‑CGGCA‑CCAA‑AGA‑TTTTTGA‑GT‑TACCGTGCAGGGCAAAACCGCCTTCCGGGAACAACGATTTCAGCGGCT‑C‑GGCGCTCAAACTGAACTC‑TTGCAGATGCTGCGCCCAGTACATCTGAATGCCGCCTTCAACAGCTGCCGGACCAAAGATaaa                  <  1:994221/238‑1 (MQ=255)                                                                                                                                                                                                                                          gtA‑A‑GG‑ATGCGGGAATCAG‑AAAG‑CCCGCCAC‑TTTTA‑CCCGATTTTCCGGGGCGGCAGTGAAGGACATCGCTAAAGAAATGCCCA‑CGGGA‑CCAA‑AAA‑TTTTTTG‑GG‑TACCGTGCAGGGAAAAACCGCCTTCCGGGAACAAAGGTTTCAGCGGCT‑C‑GGCGCTTCAACTTAACAC‑TTGCAAAACCTGCGACCCGTACAACTGCATTCCGCCTTTCACCGCTGCCGGAACCAAGATAAAATga              >  2:1011712/2‑238 (MQ=255)                                                                                                                                                                                                                                                 g‑TTGCGAGAATCAG‑CAGG‑CCCGCCAC‑TTTTA‑CCTGATTTTCCGGTGCGGCAGTGAAGTACATCGCTAAAGAAATGCCCA‑CGGCA‑CCAA‑AGA‑TTTTTGA‑GT‑TACCGTGCAGGGCAAAACCGCCTTCCGGGAACAACGATTTCAGCGGCT‑C‑GGCGCTCAAACTGAACTC‑TTGCAGATGCTGCGCCCAGTACATCTGAATGCCGCCTTCAACAGCTGCCGGACCAAAGATAAACTGTCCGt          <  1:750237/238‑1 (MQ=255)                                                                                                                                                                                                                                                       gggAATCAG‑CAAG‑CCCGCCAC‑TTTTA‑CCCGATTTTCCGGTGCGGCAGTGAAGTACATCGCTAAAGAAATGCCCA‑CGGCA‑CCAA‑AGA‑TTTTTGA‑GT‑TACCGTGCAGGGCAAAACCGCCTTCCGGGAACAACGATTTCAGCGGCT‑C‑GGCGCTCAAACTGAACTC‑TTGCAGATGCTGCGCCCAGTACATCTGAATGCCGCCTTCAACAGCTGCCGGACCAAAGATAAACTGTCCGTAGATGa    >  2:716273/1‑239 (MQ=255)                                                                                                                                                                                                                                                       gggAATCAG‑CAAG‑CCCGCCAC‑TTTTA‑CCCGATTTTCCGGTGCGGCAGTGAAGTACATCGCTAAAGAAATGCCCA‑CGGCA‑CCAA‑AGA‑TTTTTGA‑GT‑TACCGTGCAGGGCAAAACCGCCTTCCGGGAACAACGATTTCAGCGGCT‑C‑GGCGCTCAAACTGAACTC‑TTGCAGATGCTGCGCCCAGTACATCTGAATGCCGCCTTCAACAGCTGCCGGACCAAAGATAAACTGTCCGTAGATGa    >  1:482142/1‑239 (MQ=255)                                                                                                                                                                                                                                                       gggAATCAG‑CAAG‑CCCGCCAC‑TTTTA‑CCCGATTTTCCGGTGCGGCAGTGAAGTACATCGCTAAAGAAATGCCCA‑CGGCA‑CCAA‑AGA‑TTTTTGA‑GT‑TACCGTGCAGGGCAAAACCGCCTTCCGGGAACAACGATTTCAGCGGCT‑C‑GGCGCTCAAACTGAACTC‑TTGCAGATGCTGCGCCCAGTACATCTGAATGCCGCCTTCAACAGCTGCCGGACCAAAGATAAACTGTCCGTAGATGa    >  1:1192973/1‑239 (MQ=255)                                                                                                                                                                                                                                                         aaaccAG‑CAAC‑CCCGCCAC‑TTTTA‑CCCGATTTTCCGGTGCGGCAGTGAAGTACATCGCTAAAGAAATGCCCA‑CGGCA‑CCAA‑AGA‑TTTTTGA‑GT‑TACCGTGCAGGGCAAAACCGCCTTCCGGGAACAACGATTTCAGCGGCT‑C‑GGCGCTCAAACTGAACTC‑TTGCAGATGCTGCGCCCAGTACATCTGAATGCCGCCTTCAACAGCTGCCGGACCAAAGATAAACTGTCCGTAGATGaa   <  2:1165811/234‑1 (MQ=255)                                                                                                                                                                                                                                                          aaTCAG‑CAAG‑CCTGCCAC‑TTTTA‑CCCGATTTTCCGGTGCGGCAGTGAAGTACATCGCTAAAGAAATGCCCA‑CGGCA‑CCAA‑AGA‑TTTTTGA‑GT‑TACCGTGCAGGGCAAAACCGCCTTCCGGGAACAACGATTTCAGCGGCT‑C‑GGCGCTCAAACTGAACTC‑TTGCAGATGCTGCGCCCAGTACATCTGAATGCCGCCTTCAACAGCTGCCGGACCAAAGATAAACTGTCCGTAGATGAAg  <  1:140039/238‑1 (MQ=255)                                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                    TTTGGGTCAGCATCATATCCGCGTGGTTGCTGAACATCGGGATCCAGTTTTGCGGT‑AAAACCTAG‑TCAATCAGACCTCCGCCCATGTTGCCCACCACACCAAAGAGATACATTACGGTCGACATTGAGG‑CCGCCAGCACGGCGTGTACCGCAAAC‑AGCAACGGTGAAATGAACAG‑GAAGGTAAAT‑T‑CC‑AGCGGTT‑CGGTAA‑TT‑CCCA‑CCAG‑CATGGC‑GGTTA‑A‑GG‑TTGCGGGAATCAG‑CAAG‑CCCGCCAC‑TTTTA‑CCCGATTTTCCGGTGCGGCAGTGAAGTACATCGCTAAAGAAATGCCCA‑CGGCA‑CCAA‑AGA‑TTTTTGA‑GT‑TACCGTGCAGGGCAAAACCGCCTTCCGGGAACAACGATTTCAGCGGCT‑C‑GGCGCTCAAACTGAACTC‑TTGCAGATGCTGCGCCCAGTACATCTGAATGCCGCCTTCAACAGCTGCCGGACCAAAGATAAACTGTCCGTAGATGAAG  >  NC_000913/3862436‑3862905

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑