BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	65,670	T→G	12.9%	A37A (GCA→GCC)	polB ←	DNA polymerase II

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	65,670	0	T	G	12.9%	308.0 / 2.7	109	A37A (GCA→GCC)	polB	DNA polymerase II
Reads supporting (aligned to +/- strand): ref base T (16/75); new base G (15/3); total (31/78)
Fisher's exact test for biased strand distribution p-value = 1.43e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 109 total aligned reads displayed.

CATCAGATAGCGTTCTGGCGGACGCACATCGGCCTCGTAGACGGTAACGCCACCTTCACGCAGGCGCTTTTCGTAATTCATCAATTGGCGATGGGCGCGACAGTAAAGGCCATACACCGGCTGGCGGTGAAAATCCTTTAACGCCAGCGGTGTCAGGCGAAAGCCTTGTTCACCCTGCAAAATATGCTGAGCGCGGGGAACCTGATCGGCGGGAATAAACGCCACGGACTCTTGCGGTGCAAGCGTAA‑CCTG‑CAACGGCCCGTTGTCCGTCGCCAG‑CCAGAAGGAGACTTCTGTCCCTTGCGGGGTGTCCCGCCAGTGTC‑GGGTTAAGATAAAACCTGCCTGCGCCACGCTGAAAATCCATCAAAAAA‑CCA‑GGCTTGAGTATA‑G‑CCT‑GGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCTTCGCGCCATGCTTACGCAGATAGTGTTTATCCA  >  NC_000913/65433‑65907

cATCAGATAGCGTTCTGGCGGACGCACATCGGCCTCGTAGACGGTAACGCCACCTTCACGCAGGCGCTTTTCGTAATTCATCAATTGGCGATGGGCGCGACAGTAAAGGCCATACACCGGCTGGCGGTGAAAATCCTTTAACGCCAGCGGTGTCAGGCGAAAGCCTTGTTCACCCTGCAAAATATGCTGAGCGCGGGGAACCTGATCGGCGGGAATAAACGCCACGGACTCTTGCGGTg                                                                                                                                                                                                                                                       <  2:704172/239‑1 (MQ=255)

       tAGCGTTCTGGCGGACGCACATCGGCCTCGTAGACGGTAACGCCACCTTCACGCAGGCGCTTTTCGTAATTCATCAATTGGCGATGGGCGCGACAGTAAAGGCCATACACCGGCTGGCGGTGAAAATCCTTTAACGCCAGCGGTGTCAGGCGAAAGCCTTGTTCACCCTGCAAAATATGCTGAGCGCGGGGAACCTGATCGGCGGGAATAAACGCCACGGACTCTTGCGGTGCAAGc                                                                                                                                                                                                                                                  <  2:31672/237‑1 (MQ=255)

       tAGCGTTCTGGCGGACGCACATCGGCCTCGTAGACGGTAACGCCACCTTCACGCAGGCGCTTTTCGTAATTCATCAATTGGCGATGGGCGCGACAGTAAAGGCCATACACCGGCTGGCGGTGAAAATCCTTTAACGCCAGCGGTGTCAGGCGAAAGCCTTGTTCACCCTGCAAAATATGCTGAGCGCGGGGAACCTGATCGGCGGGAATAAACGCCACGGACTCTTGCGGTGCAAGCg                                                                                                                                                                                                                                                 <  2:817537/238‑1 (MQ=255)

          cGTTCTGGCGGACGCACATCGGCCTCGTAGACGGTAACGCCACCTTCACGCAGGCGCTTTTCGTAATTCATCAATTGGCGATGGGCGCGACAGTAAAGGCCATACACCGGCTGGCGGTGAAAATCCTTTAACGCCAGCGGTGTCAGGCGAAAGCCTTGTTCACCCTGCAAAATATGCTGAGCGCGGGGAACCTGATCGGCGGGAATAAACGCCACGGACTCTTGCGGGGCAAGCGTaa                                                                                                                                                                                                                                              >  1:282793/1‑238 (MQ=255)

           gTTCTGGCGGACGCACATCGGCCTCGTAGACGGTAACGCCACCTTCACGCAGGCGCTTTTCGTAATTCATCAATTGGCGATGGGCGCGACAGTAAAGGCCATACACCGGCTGGCGGTGAAAATCCTTTAACGCCAGCGGTGTCAGGCGAAAGCCTTGTTCACCCTGCAAAATATGCTGAGCGCGGGGAACCTGATCGGCGGGAATAAACGCCACGGACTCTTGCGGTGCAAGCGTAA‑c                                                                                                                                                                                                                                            <  1:208696/238‑1 (MQ=255)

            ttCTGGCGGACGCACATCGGCCTCGTAGACGGTAACGCCACCTTCACGCAGGCGCTTTTCGTAATTCATCAATTGGCGATGGGCGCGACAGTAAAGGCCATACACCGGCTGGCGGTGAAAATCCTTTAACGCCAGCGGTGTCAGGCGGAAGCCTTGTTCACCCTGCAAAATATGCTGAGCGCGGGGGACCTGATCGGCGGGGAAAAACGCCACCGACTCTTGCGGGGGCAGCGCAA‑ccg                                                                                                                                                                                                                                          >  2:67528/1‑238 (MQ=255)

                 gCGGACGCACATCGGCCTCGTAGACGGTAACGCCACCTTCACGCAGGCGCTTTTCGTAATTCATCAATTGGCGATGGGCGCGACAGTAAAGGCCATACACCGGCTGGCGGTGAAAATCCTTTAACGCCAGCGGGGTCAGGCGAAAGCCTTGTTTACCCTGCAAAATATTCTGAGCGCGGGGAACCTGATCGGCGGGAGACACCCCCACCGACCGTTGCGCGGCAAGCCGAA‑CCTG‑CCAc                                                                                                                                                                                                                                    >  2:1072640/1‑239 (MQ=255)

                    gACGCACATCGGCCTCGTAGACGGTAACGCCACCTTCACGCAGGCGCTTTTCGTAATTCATCAATTGGCGATGGGCGCGACAGTAAAGGCCATACACCGGCTGGCGGTGAAAATCCTTTAACGCCAGCGGTGTCAGGCGAAAGCCTTGTTCACCCTGCAAAATATGCTGAGCGCGGGGAACCTGATCGGCGGGGAAAAAAGCCCCGGAGCCCTGCGGGGGAAGCGTAA‑ACCG‑CAACgg                                                                                                                                                                                                                                  >  2:365581/1‑238 (MQ=255)

                      cGCACATCGGCCTCGTAGACGGTAACGCCACCTTCACGCAGGCGCTTTTCGTAATTCATCAATTGGCGATGGGCGCGACAGTAAAGGCCATACACCGGCTGGCGGTGAAAATCCTTTAACGCCAGCGGTGTCAGGCGAAAGCCTTGTTCACCCTGCAAAATATGCTGAGCGCGGGGAACCTGATCGGCGGGGAAAAAAGCCCCCGGCCCTTGCGGGGGAAGGGGAA‑CCTG‑CCACgggc                                                                                                                                                                                                                                >  2:277906/1‑236 (MQ=255)

                                  tCGTAGACGGTAACGCCACCTTCACGCAGGCGCTTTTCGTAATTCATCAATTGGCGATGGGCGCGACAGTAAAGGCCATACACCGGCTGGCGGTGAAAATCCTTTAACGCCAGCGGTGTCAGGCGAAAGCCTTGTTCACCCTGCAAAATATGCTGAGCGCGGGGAACCTGATCGGCGGGAATAAACGCCACGGACTCTTGCGGTGCAAGCGTAA‑CCTG‑CAACGGCCCGTTGTCCGTCgc                                                                                                                                                                                                                   <  2:440395/239‑1 (MQ=255)

                                          ggTAACGCCACCTTCACGCAGGCGCTTTTCGTAATTCATCAATTGGCGATGGGCGCGACAGTAAAGGCCATACACCGGCTGGCGGTGAAAATCCTTTAACGCCAGCGGTGTCAGGCGAAAGCCTTGTTCACCCTGCAAAATATGCTGAGCGCGGGGAACCTGATCGGCGGGAAAAAACGCCCCGGACCCTTTCGGGGCAAGCGCAA‑CCCGCCAACGGCCC‑TTGTCCGTCgcccccccga                                                                                                                                                                                                           >  2:911048/1‑232 (MQ=255)

                                              aCGCCCCCTTCACGCAGGCGCTTTGCGTAATTCATCAATTGGCGATGGGCGCGACAGTAAAGGCCATACACCGGCTGGAGGTTAAAAGCCTTTAACGCCAGCGGTGTCAGGCGAAAGCCTTGTTCACCCTGCAAAATATGCTGAGCGCGGGGAACCTGATCGGCGGGAATAAACGCCACGGACTCTTGCGGTGCAAGCGTAA‑CCTG‑CAACGGCCCGTTGTCCGTCGCCAG‑CCAGAAGga                                                                                                                                                                                                      <  2:1277717/239‑1 (MQ=255)

                                              aCGCCACCTTCACGCAGGCGCTTTTCGTAATTCATCAATTGGCGATGGGCGCGACAGTAAAGGCCATACACCGGCTGGCGGTGAAAATCCTTTAACGCCAGCGGTGTCAGGCGAAAGCCTTGTTCACCCTGCAAAATATGCTGAGCGCGGGGAACCTGATCGGCGGGGATAAACGCCACGGACTCTTTCGGTGCAAGCGGAA‑CCTG‑CAACGGCCCGTTGTTCGGCGCCAG‑CCCAAAGga                                                                                                                                                                                                      >  1:344693/1‑239 (MQ=255)

                                              aCGCCACCTTCACGCAGGCGCTTTTCGTAATTCATCAATTGGCGATGGGCGCGACAGTAAAGGCCATACACCGGCTGGCGGTGAAAATCCTTTAACGCCAGCGGTGTCAGGCGAAAGCCTTGTTCACCCTGCAAAATATGCTGAGCGCGGGGAACCTGATCGGCGGGAATAAACGCCACGGACTCTTGCGGTGCAAGCGTAA‑CCTG‑CAACGGCCCGTTGTCCGTCGCCAG‑CCAGAAGga                                                                                                                                                                                                      <  2:1013099/239‑1 (MQ=255)

                                              aCGCCACCTTCACGCAGGCGCTTTTCGTAATTCATCAATTGGCGATGGGCGCGACAGTAAAGGCCATACACCGGCTGGCGGTGAAAATCCTTTAACGCCAGCGGTGTCAGGCGAAAGCCTTGTTCACCCTGCAAAATATGCTGAGCGCGGGGAACCTGATCGGCGGGAATAAACGCCACGGACTCTTGCGGTGCAAGCGTAA‑CCTG‑CAACGGCCCGTTGTCCGTCGCCAG‑CCAGAAGga                                                                                                                                                                                                      <  1:397378/239‑1 (MQ=255)

                                              aCGCCACCTTCACGCAGGCGCTTTTCGTAATTCATCAATTGGCGATGGGCGCGACAGTAAAGGCCATACACCGGCTGGCGGTGAAAATCCTTTAACGCCAGCGGTGTCAGGCGAAAGCCTTGTTCACCCTGCAAAATATGCTGAGCGCGGGGAACCTGATCGGCGGGAATAAACGCCACGGACTCTTGCGGTGCAAGCGTAA‑CCTG‑CAACGGCCCGTTGTCCGTCGCCAG‑CCAGAAGga                                                                                                                                                                                                      <  2:777251/239‑1 (MQ=255)

                                              aCGCCACCTTCACGCAGGCGCTTTTCGTAATTCATCAATTGGCGATGGGCGCGACAGTAAAGGCCATACACCGGCTGGCGGTGAAAATCCTTTAACGCCAGCGGTGTCAGGCGAAAGCCTTGTTCACCCTGCAAAATATGCTGAGCGCGGGGAACCTGATCGGCGGGAATAAACGCCACGGACTCTTGCGGTGCAAGCGTAA‑CCTG‑CAACGGCCCGTTGTCCGTCGCCAG‑CCAGAAGga                                                                                                                                                                                                      <  2:1242034/239‑1 (MQ=255)

                                              aCGCCACCTTCACGCAGGCGCTTTTCGTAATTCATCAATTGGCGATGGGCGCGACAGTAAAGGCCATACACCGGCTGGCGGTGAAAATCCTTTAACGCCAGCGGTGTCAGGCGAAAGCCTTGTTCACCCTGCAAAATATGCTGAGCGCGGGGAACCTGATCGGCGGGAATAAACGCCACGGACTCTTGCGGTGCAAGCGTAA‑CCTG‑CAACGGCCCGTTGTCCGTCGCCAG‑CCAGAAGga                                                                                                                                                                                                      <  1:487175/239‑1 (MQ=255)

                                              aCGCCACCTTCACACAGGCGCTTTTCGTAATTCATCAATTGGCGATGGGCGCGACAGTAAAGGCCATACACCGGCTGGCGGTGAAAATCCTTTAACGCCAGCGGTGTCAGGCGAAAGCCTTGTTCACCCTGCAAAATATGCTGAGCGCGGGGAACCTGATCGGCGGGAATAAACGCCACGGACTCTTGCGGTGCAAGCGTAA‑CCTG‑CAACGGCCCGTTGTCCGTCGCCAG‑CCAGAAGga                                                                                                                                                                                                      <  1:255337/239‑1 (MQ=255)

                                                     cTTCACGCAGGCGCTTTTCGTAATTCATCAATTGGCGATGGGCGCGACAGTAAAGGCCATACACCGGCTGGCGGTGAAAATCCTTTAACGCCAGCGGTGTCAGGCGAAAGCCTTGTTCACCCTGCAAAATATGCTGAGCGCGGGGAACCTGATCGGCGGGAATAAACGCCACGGACTCTTGCGGTGCAAGCGTAA‑CCTG‑CAACGGCCCGTTGTCCGTCGCCAG‑CCAGAAGGAGACTTCt                                                                                                                                                                                               <  1:41114/239‑1 (MQ=255)

                                                       tCACGCAGGCGCTTTTCGTAATTCATCAATTGGCGATGGGCGCGACAGTAAAGGCCATACACCGGCTGGCGGTGAAAATCCTTTAACGCCAGCGGTGTCAGGCGAAAGCCTTGTTCACCCTGCAAAATATGCTGAGCGCGGGGAACCTGATCGGCGGGAATAAACGCCACGGACTCTTGCGGTGCAAGCGTAA‑CCTG‑CAACGGCCCGTTGTCCGTCGCCAG‑CCAGAAGGAGACTTCTGt                                                                                                                                                                                             <  2:130046/239‑1 (MQ=255)

                                                        cACGCAGGCGCTTTTCGTAATTCATCAATGGGCGATGGGCGCGACAGTAAAGGCCATACACCGGCTGGCGGTGAAAATCCTTTAACGCCAGCGGTGTCAGGCGAAAGCCTTGTTCACCCTGCAAAATATGCTGAGCGCGGGGAACCTGATCGGCGGGAATAAACGCCACGGACTCTTGCGGTGCAAGCGTAA‑CCTG‑CAACGGCCCGTTGTCCGTCGCCAG‑CCAGAAGGAGACTTCTGt                                                                                                                                                                                             <  1:1190819/238‑1 (MQ=255)

                                                              ggCGCTTTTCGTAATTCATCAATTGGCGATGGGCGCGACAGTAAAGGCCATACACCGGCTGGCGGTGAAAATCCTTTAACGCCAGCGGTGTCAGGCGAAAGCCTTGTTCACCCTGCAAAATATGCTGAGCGCGGGGAACCTGATCGGCGGGGATAAACCCCCCGGACCCTTGCGGTGGCAGGGGAA‑ACCC‑CAACAGCCCGGTGGTCGTCGCCAG‑CCAGAAGGGGACGTCTGTCCCTTGc                                                                                                                                                                                      >  2:788263/1‑239 (MQ=255)

                                                               gcgcTTTTCGTAATTCATCAATTGGCGATGGGCGCGACAGTAAAGGCCATACACCGGCTGGCGGTGAAAATCCTTTAACGCCAGCGGTGTCAGGCGAAAGCCTTGTTCACCCTGCAAAATATGCTGAGCGCGGGGAACCTGATCGGCGGGGATAAAAGCCACGGACCCTTTCGGGGGCAGCGTAA‑CCCG‑CCACGGCCCCGTGTCCGTCGCCAC‑CCAGAAAGAGACATCTGTCCCCTGCg                                                                                                                                                                                     >  2:26235/1‑239 (MQ=255)

                                                                 gcTTTTCGTAATTCATCAATTGGCGATGGGCGCGACAGTAAAGGCCATACACCGGCTGGCGGTGAAAATCCTTTAACGCCAGCGGTGTCAGGCGAAAGCCTTGTTCACCCTGCAAAATATGCTGAGCGCGGGGAACCTGATCGGCGGGAATAAACGCTACGGACTCTTGCGGTGCAAGCGTAA‑CCTG‑CAACGGCCCGATGTCCGTCGCCAG‑CCAGAAGGAGACTTCTGTCCCTTGCgg                                                                                                                                                                                    <  1:37665/238‑1 (MQ=255)

                                                                   ttttCGTAATTCATCAATTGGCGATGGGCGCGACAGTAAAGGCCATACACCGGCTGGCGGTGAAAATCCTTTAACGCCAGCGGTGTCAGGCGAAAGCCTTGTTCACCCTGCAAAATATGCTGAGCGCGGGGAACCTGATCGGCGGGAATAAACGCCACGGACTCTTGCGGTGCAAGCGTAA‑CCTG‑CAACGGCCCGTTGTCCGTCGCCAG‑CCAGAAGGAGACTTCTGTCCCTTGCGGGgt                                                                                                                                                                                 <  1:365581/239‑1 (MQ=255)

                                                                          aaTTCATCAATTGGCGATGGGCGCGACAGTAAAGGCCATACACCGGCTGGCGGTGAAAATCCTTTAACGCCAGCGGGGTCAGGCGAAAGCCTTGTTCACCCTGCAAAATATGCTGAGCGCGGGGAACCTGATCGGCGGGAATAAACGCCACGGACTCTTGCGGTGCAAGCGTAA‑CCTG‑CAACGGCCCGTTGTCCGTCGCCAG‑CCAGAAGGAGACTTCTGTCCCTTGCGGGGTGTCCCg                                                                                                                                                                           <  1:299837/238‑1 (MQ=255)

                                                                          aaTTCATCAAGTGGCGAGGGGCGCGACAGTAAAGGCCATACACCGGCTGGCGGTAAAAATCCTTTAACGCCAGCGGTGTCAGGCGAAAGCCTTGTTCCCCCTGAAAAATATGCTGAGCGCGGGGAACCTGATCGGCGGAAATAAACGCCACGGACTCTTGCGGTGCAAGCGTAA‑CCTG‑CAACGGCCCGTTGTCCGTCGCCAG‑CCAGAAGGAGCCTTCTGTCCCTTGCGGGGTGTCCCGc                                                                                                                                                                          <  2:1016649/239‑1 (MQ=255)

                                                                               atcaATTGGCGATGGGCGCGACAGTAAAGGCCATACACCGGCTGGCGGTGAAAATCCTTTAACGCCAGCGGTGTCAGGCGAAAGCCTTGTTCACCCTGCAAAATATGCTGAGCGCGGGGAACCTGATCGGCGGGAATAAACGCCACGGACTCTTGCGGTGCAAGCGTAA‑CCTG‑CAACGGCCCGTTGTCCGTCGCCAG‑CCAGAAGGAGACTTCTGTCCCTTGCGGGGTGTCCCGCCAgtg                                                                                                                                                                     <  2:239546/239‑1 (MQ=255)

                                                                                tcaATTGGCGATGGGCGCGACAATAAAGGCCATACACCGGCTGGCGGTGAAAATCCTTTAACGCCAGCGGTGTCAGGCGAAAGCCTTGTTCACCCTGCAAAATATGCTGAGCGCGGGGAACCTGATCGGCGGGAATAAACGCCACGGACTCTTGCGGTGCAAGCGTAA‑CCTG‑CAACGGCCCGTTGTCCGTCGCCAG‑CCAGAAGGAGACTTCTGTCCCTTGCGGGGTGTCCCGCCAgtgt                                                                                                                                                                    <  1:863883/239‑1 (MQ=255)

                                                                                ccaATTGGCGATGGGCGCGACAGTAAAGGCCATACACCGGCTGGCGGTGAAAATCCTTTAACGCCAGCGGTGTCAGGCGAAAGCCTTGTTCACCCTGCAAAATATGCTGAGCGCGGGGAACCTGATCGGCGGGAATAAACGCCACGGACTCTTGCGGTGCAAGCGTAA‑CCTG‑CAACGGCCCGTTGTCCGTCGCCAG‑CCAGAAGGAGACTTCTGTCCCTTGCGGGGTGTCCCGCCAgtgt                                                                                                                                                                    <  1:814940/238‑1 (MQ=255)

                                                                                    ttGGCGATGGGCGCGACAGTAAAGGCCATACACCGGCTGGCGGTGAAAATCCTTTAACGCCAGCGGTGTCAGGCGAAAGCCTTGTTCACCCTGCAAAATATGCTGAGCGCGGGGAACCTGATCGGCGGGAATAAACACCACCGGCTCTTTCGGGGGAAAAGCAACCCCC‑CAAGGGCCCGTT‑TCCGTCCCCCG‑CCCGAACGGGACTTCTGTCCCCTGCGGGGGGTCCCGCCCGTGTC‑ggg                                                                                                                                                               >  2:579222/1‑239 (MQ=255)

                                                                                       gCGATGGGCGCGACAGTAAAGGCCATACACCGGCTGGCGGTGAAAATCCTTTAACGCCAGCGGTGTCAGGCGAAAGCCTTGTTCACCCTGCAAAATATGCTGAGCGCGGGGAACCTGATCGGCGGGAATAAAAGCCCCCGGCTCTTGCGGGGGAAACGTAA‑ACCG‑CCACCGCCCCCTGTCCCTCCCCCGCCCAGAGGGGGAC‑TCTTTCCCCTTCGGGGGGGCCCCCCCGGGGC‑ggggtg                                                                                                                                                            >  2:1108408/1‑236 (MQ=255)

                                                                                                            gCCATACACCGGCTGGCGGTGAAAATCCTTTAACGCCAGCGGTGTCAGGCGAAAGCCTTGTTCACCCTGCAAAATATGCTGAGCGCGGGGAACCTGATCGGCGGGAATAAACGCCCCGGACTCTTTCGGGGGAAGCGTAA‑ACTG‑CCACGGCCCCTTTTCCGTCGCCCA‑CCCGAAGGGGACTTCTTTCCCCTGCGGGGTGTCCCCCCCGTGGC‑GGGTTTAGATAAAACCTGCCTCCGcc                                                                                                                                        >  2:591459/1‑238 (MQ=255)

                                                                                                             ccATACACCGGCTGGCGGTGAAAATCCTTTAACGCCAGCGGTGTCAGGCGAAAGCCTTGTTCACCCTGCAAAATATGCTGAGCGCGGGGAACCTGATCGGCGGGAATAAACGCCACGGACTCTTGCGGTGCAAGCGTAA‑CCTG‑CAACGGCCCGTTGTCCGTCGCCAG‑CCAGAAGGAGACTTCTGTCCCTTGCGGGGTGTCCCGCCAGTGTC‑GGGTTAAGATAAAACCTGCCTGCGCCa                                                                                                                                       <  1:322698/238‑1 (MQ=255)

                                                                                                               aTACACCGGCTGGCGGTGAAAATCCTTTAACGCCAGCGGTGTCAGGCGAAAGCCTTGTTCACCCTGCAAAATATGCTGAGCGCGGGGAACCTGATCGGCGGGAATAAACGCCACGGACTCTTGCGGGGCAAGCGGAA‑CCTG‑CAACGGCCCGCTGTCCGGCGCCAG‑CCCGAAGGAGACTTCTGTCCCTTGCGGGGTGTCCCGCCAGTGTC‑GGGGTAAGATAAAACCCGCCTGCGCCCCg                                                                                                                                     >  1:518784/1‑238 (MQ=255)

                                                                                                                            cGGTGAAAATCCTTTAACGCCAGCGGTGTCAGGCGAAAGCCTTGTTCACCCTGCAAAATATGCTGAGCGCGGGGAACCTGATCGGCGGGAATAAACGCCACGGACTCTTGCGGTGCAAGCGTAA‑CCTG‑CAACGGCCCGTTGTCCGTCGCCAG‑CCAGAAGGAGACTTCTGTCCCTTGCGGGGTGTCCCGCCAGTGTC‑GGGTTAAGATAAAACCTGCCTGCGCCACGCTGAAAATCCATc                                                                                                                        <  1:370207/238‑1 (MQ=255)

                                                                                                                                  aaaTCCTTTAACGCCAGCGGTGTCAGGCGAAAGCCTTGTTCACCCTGCAAAATATGCTGAGCGCGGGGAACCTGATCGGCGGGAATAAACGCCACGGACTCTTGCGGTGCAAGCGTAA‑CCTG‑CAACGGCCCGTTGCCCGTCGCCAG‑CCAGAAGGAGACTTCTGTCCCTTGCGGGGTGTCCCGCCAGTGTC‑GGGTTAAGATAAAACCTGCCTGCGCCACGCTGAAAATCCATCAAAAAA‑c                                                                                                                <  1:67528/239‑1 (MQ=255)

                                                                                                                                    aTCCTTTAACGCCAGCGGTGTCAGGCGAAAGCCTTGTTCACCCTGCAAAATATGCTGAGCGCGGGGAACCTGATCGGCGGGAATAAACGCCACGGACTCTTGCGGTGCAAGCGTAA‑CCTG‑CAACGGCCCGTTGTCCGTCGCCAG‑CCAGAAGGAGACTTCTGTCCCTTGCGGGGTGTCCCGCCAGTGTC‑GGGTTAAGATAAAACCTGCCTGCGCCACGCTGAAAATCCATCAAAAAA‑CCa                                                                                                              <  2:481280/239‑1 (MQ=255)

                                                                                                                                    aTCCTTTAACGCCAGCGGTGTCAGGCGAAAGCCTTGTTCACCCTGCAAAATATGCTGAGCGCGGGGAACCTGATCGGCGGGAATAAACGCCACGGACTCTTGCGGTGCAAGCGTAA‑CCTG‑CAACGGCCCGTTGTCCGTCGCCAG‑CCAGAAGGAGACTTCTGTCCCTTGCGGGGTGTCCCGCCAGTGTC‑GGGTTAAGATAAAACCTGCCTGCGCCACGCTGAAAATCCATCAAAAAA‑CCa                                                                                                              <  1:833218/239‑1 (MQ=255)

                                                                                                                                    aTCCTTTAACGCCAGCGGTGTCAGGCGAAAGCCTTGTTCACCCTGCAAAATATGCTGAGCGCGGGGAACCTGATCGGCGGGAATAAACGCCACGGACTCTTGCGGTGCAAGCGTAA‑CCTG‑CAACGGCCCGTTGTCCGTCGCCAG‑CCAGAAGGAGACTTCTGTCCCTTGCGGGGTGTCCCGCCAGTGTC‑GGGTTAAGATAAAACCTGCCTGCGCCACGCTGAAAATCCATCAAAAAA‑CCa                                                                                                              <  1:63993/239‑1 (MQ=255)

                                                                                                                                    aTCCTTTAACGCCAGCGGTGTCAGGCGAAAGCCTTGTTCACCCTGCAAAATATGCTGAGCGCGGGGAACCTGATCGGCGGGAATAAACGCCACGGACTCTTGCGGTGCAAGCGTAA‑CCTG‑CAACGGCCCGTTGTCCGTCGCCAG‑CCAGAAGGAGACTTCTGTCCCTTGCGGGGTGTCCCGCCAGTGTC‑GGGTTAAGATAAAACCTGCCTGCGCCACGCTGAAAATCCATCAAAAAA‑CCa                                                                                                              <  1:1108408/239‑1 (MQ=255)

                                                                                                                                       cTTTAACGCCAGCGGTGTCAGGCGAAAGCCTTGTTCACCCTGCAAAATATGCTGAGCGCGGGGAACCTGATCGGCGGGAATAAACGCCACGGACTCTTGCGGTGCAAGCGTAA‑CCTG‑CAACGGCCCGTTGTCCGTCGCCAG‑CCAGAAGGAGACTTCTGTCCCTTGCGGGGTGTCCCGCCAGTGTC‑GGGTTAAGATAAAACCTGCCTGCGCCACGCTGAAAATCCATCAAAAAA‑CCA‑GGc                                                                                                          <  1:374158/239‑1 (MQ=255)

                                                                                                                                       cTTTAACGCCAGCGGTGTCAGGCGAAAGCCTTGTTCACCCTGCAAAATATGCTGAGCGCGGGGAACCTGATCGGCGGGAATAAACGCCACGGACTCTTGCGGTGCAAGCGTAA‑CCTG‑CAACGGCCCGTTGTCCGTCGCCAG‑CCAGAAGGAGACTTCTGTCCCTTGCGGGGTGTCCCGCCAGTGTC‑GGGTTAAGATAAAACCTGCCTGCGCCACGCTGAAAATCCATCAAAAAA‑CCA‑GGc                                                                                                          <  1:1133296/239‑1 (MQ=255)

                                                                                                                                        tttAACGCCAGCGGTGTCAGGCGAAAGCCTTGTTCACCCTGCAAAATATGCTGAGCGCGGGGAACCTGATCGGCGGGAATAAACGCCACGGACTCTTGCGGTGCAAGCGTAA‑CCTG‑CAACGGCCCGTTGTCCGTCGCCAG‑CCAGAAGGAGACTTCTGTCCCTTGCGGGGTGTCCCGCCAGTGTC‑GGGTTAAGATAAAACCTGCCTGCGCCACGCTGAAAATCCATCAAAAAA‑CCA‑GGCt                                                                                                         <  1:179813/239‑1 (MQ=255)

                                                                                                                                        ttgaaCGCCAGCGGTGTCAGGCGAAGGCCGTGTCCACCCTGCAAACGGTGCTGAGGGGGGGGAACCTGATCGTCGGCACTAAACGCCGCGGACTCTTGCGGGGCAACCGTAC‑CTTG‑CACCGGCCCGTTGTCCGTCGCCAG‑CCAGAAGGAGACTTCTGTCCCTTGCGGGGTGCGCCGCCAGTGTC‑GGGTTAAGATAAAACCTGCCTGCGCCACGCTG‑AAATCCATCAAAAAA‑CCA‑GGCtt                                                                                                        <  2:450340/236‑1 (MQ=255)

                                                                                                                                             cGCCAGCGGTGTCAGGCGAAAGCCTTGTTCACCCTGCAAAATATGCTGAGCGCGGGGAACCTGATCGGCGGGAATAAACGCCACGGACTCTTGCGGTGCAAGCGTAA‑CCTG‑CAACGGCCCGTTGTCCGTCGCCAG‑CCAGAAGGAGACTTCTGTCCCTTGCGGGGTGTCCCGCCAGTGTC‑GGGTTAAGATAAAACCTGCCTGCGCCACGCTGAAAATCCATCAAAAAA‑CCA‑GGCTTGAGt                                                                                                    <  1:1018365/239‑1 (MQ=255)

                                                                                                                                              gCCAGCGGTGTCAGGCGAAAGCCTTGTTCACCCTGCAAAATATGCTGAGCGCGGGGAACCTGATCGGCGGGAATAAACGCCACGGACTCTTGCGGTGCAAGCGTAA‑CCTG‑CAACGGCCCGTTGTCCGTCGCCAG‑CCAGAAGGAGACTTCTGTCCCTTGCGGGGTGTCCCGCCAGTGTC‑GGGTTAAGATAAAACCTGCCTGCGCCACGCTGAAAATCCATCAAAAAA‑CCA‑GGCTTGAGta                                                                                                   <  1:788263/239‑1 (MQ=255)

                                                                                                                                                        tCAGGCGAAAGCCTTGTTCACCCTGCAAAATATGCTGAGCGCGGGGAACCTGATCGGCGGGAATAAACGCCACGGACTCTTGCGGTGCAAGCGTAA‑CCTG‑CAACGGCCCGTTGTCCGTCGCCAG‑CCAGAAGGAGACTTCTGTCCCTTGCGGGGTGTCCCGCCAGTGTC‑GGGTTAAGATAAAACCTGCCTGCGCCACGCTGAAAATCCATCAAAAAA‑CCA‑GGCTTGAGTATA‑G‑CCT‑GGt                                                                                       <  1:911048/238‑1 (MQ=255)

                                                                                                                                                             cGAAAGCCTTGTTCACCCTGCAAAATATGCTGAGCGCGGGGAACCTGATCGGCGGGAATAAAACCCCCGGACTCTTGCGGTGCAAGCGTAA‑ACTG‑CAACGGCCCGGTGTCCGTCGCCAG‑CCCGAAGGAGGCTTCTGTCCCCTTCGGGGGGGCCCGCCCGGGGCGGGGTTAAAAAAAAACC‑GCCTGCGCCCCCCGGGAAAACCATCAAAAAA‑ACA‑GGGTTGAGAATATG‑CCG‑GGtttgtt                                                                                  >  2:224483/1‑236 (MQ=255)

                                                                                                                                                               aaaGCCTTGTTCACCCTGCAAAATATGCTGAGCGCGGGGAACCTGATCGGCGGGAATAAACGCCACGGGCTCTTGCGGTGCAAGCGTAA‑CCTG‑CAACGGCCCGTTGTCCGTCGCCAC‑CCAGAAGGAGACTTCTGTCCCTTGCGGGGGGTCCCCCCAGTGGCGGGGTTAAG‑GAAAAACTGCCCTCGCCCCGCTGAAAAACCATCAAAAAACCCA‑GCCTTG‑GTAAA‑GCCCT‑GGttt‑gtttg                                                                               >  2:1188080/1‑239 (MQ=255)

                                                                                                                                                                  gCCTTGTTCACCCTGCAAAATATGCTGAGCGCGGGGAACCTGATCGGCGGGAATAAACGCCACGGACTCTTGCGGTGCAAGCGTAA‑CCTG‑CAACGGCCCGTTGTCCGTCGCCAG‑CCAGAAGGAGACTTCTGTCCCTTGCGGGGGGTCCCGCCCGTGGC‑GGGGTTAGATAAAAACCTCCCGCGCCACGCTGGAAAACCCACCAAAAA‑CCA‑GGCGTTGGGATT‑A‑CCC‑GGTTTTgtttgttt                                                                            >  2:147273/1‑239 (MQ=255)

                                                                                                                                                                       gTTCACCCTGCAAAATATGCTGAGCGCGGGGAACCTGATCGGCGGGAATAAACGCCACGGACTCTTGCGGTGCAAGCGTAA‑CCTG‑CAACGGCCCGTTGTCCGTCGCCAG‑CCAGAAGGAGACTTCTGTCCCTTGCGGGGTGTCCCGCCAGTGTC‑GGGTTAAGATAAAACCTGCCTGCGCCACGCTGAAAATCCATCAAAAAA‑CCA‑GGCTTGAGTATA‑G‑CCT‑GGTTTCGTTTGATTGGCtg                                                                       <  1:277906/239‑1 (MQ=255)

                                                                                                                                                                       gTTCACCCTGCAAAATAGGCTGAGCGGGGGGAACCTGATCGGCGGGAATAAACGCCAGGGACTCTTGCGGTGAAAGCGTAA‑CCTG‑AAACGGCCCGTTGTCGGTCGCCAG‑CCAGAAGGAGACTACTGTCCCTTGCGGGGTGTCCCGGCAGTGTC‑GGGTTAAGATAAAACCTGCCTGCGCCACGCTGAAAATCCATCAAAAAA‑CCA‑GGCTTGAGTATA‑G‑CCT‑GGTTTCGTTTGATTGGCtg                                                                       <  1:977219/239‑1 (MQ=255)

                                                                                                                                                                       gTTCACCCGGCAAAATATGCCGAGCGGGGGGAGCCTGATCGGGGGGAATAAACGCCACGGACTCTTGCGGTGCAAGCGTAC‑CCTG‑CAACGGCCCGTTGTCCGTCGCCAG‑CCAGAAGGAGACTTCTGTCCCTTGCGGGGTGTCCCGCCAGTGTC‑GGGTTAAGATAAAACCTGCCTGCGCCACGCTGAAAATCCATCAAAAAA‑CCA‑GGCTTGAGTATA‑G‑CCT‑GGTTTCGTTTGATTGGCtg                                                                       <  2:515309/239‑1 (MQ=255)

                                                                                                                                                                       gTTCACCCGGCAAAAAATGCTGAGCGGGGGGAACCTGATCGGCGGGAATAAACGCCCCGGACTCTTGCGGTGCAAGCGTAC‑CCTG‑CAACGGCCCGTTGTCCGTCGCCAG‑CCAGAAGGAGACTTCTGCCCCTTGCGGGGTGTCCCGCCAGTGTC‑GGGTTAAGATAAAACCTGCCTGCGCCACGCTGAAAATCCATCAAAAAA‑CCA‑GGCTTGAGTATA‑G‑CCT‑GGTTTCGTTTGATTGGCtg                                                                       <  2:518784/239‑1 (MQ=255)

                                                                                                                                                                             ccTGCAAAATATGCTGAGCGCGGGGAACCTGATCGGCGGGAATAAACGCCACGGACTCTTGCGGTGCAAGCGTAA‑CCTG‑CAACGGCCCGTTGTCCGTCGCCAG‑CCAGAAGGAGACTTCTGTCCCTTTCGGGGTGTCCCGCCAGTGTC‑GGGGTAAGATAAAAACTGTCCGCGCCACGCTGAAAATCCATCCAAAAA‑CCC‑GGCTTCGGGATA‑A‑CCT‑GGTTTTGTTTTTTTGGCTGGGGgtt                                                                 >  2:653722/1‑239 (MQ=255)

                                                                                                                                                                             ccTGCAAAATATGCTGAGCGCGGGGAACCTGATCGGCGGGAATAAACGCCACGGACTCTTGCGGGGCAAGCGTAA‑CCTG‑CAACGGCCCGTTGTCCGTCGCCAG‑CCAGAAGGAGACTTCTGTCCCTTGCGGGGGGTCCCGCCAGTGTC‑GGGGTAAAAAAAAACCTGCCCGCGCCCCGCTGGAAATCCATCAAAAAA‑AAA‑AGGTTGAGGATA‑G‑CCC‑GGGTTCTTTTTAtttggtttgggtt                                                                 >  2:275577/1‑228 (MQ=255)

                                                                                                                                                                              cTGCAAAATATGCTGAGCGCGGGGAACCTGATCGGCGGGAATAAACGCCACGGACTCTTGCGGTGCAAGCGTAA‑CCTG‑CAACGGCCCGTTGTCCGTCGCCAG‑CCAGAAGGAGACTTCTGTTCCCTGCGGGGGGTCCCGCCCGTGTC‑GGGTTAAAATAAAACCCGCCTGCGCCCCGCTGGAAATCCCTCAAAAAA‑CCA‑GGCTTGAGTATA‑G‑CCT‑GGTTTCGTTTTGTTGGGCTTGGtttt                                                                >  2:1057315/1‑239 (MQ=255)

                                                                                                                                                                               ggCAAAATATGCTGAGCGCGGGGACCCTGATCGGCGGGAATAACCGCCACGGCCTCTTGCGGTGCAAGCGTAC‑CCTG‑CAACGGCCCGTTGTCCGTCGCCAG‑CCAGAAGGAGACTTCTGTCCCTTGCGGGGTGTCCCGCCAGTGTC‑GGGTTAAGATAAAACCTGCCTGCGCCACGCTGAAAATCCATCAAAAAA‑CCA‑GGCTTGAGTATA‑G‑CCT‑GGTTTCGTTTGATTGGCTGTGGTTTta                                                               <  2:1156825/238‑1 (MQ=255)

                                                                                                                                                                                gCAAATTATGCTGAGCGCGGGGAACTTGATCGGCGGGAATAAACGCCCCGGACTCTTGCGGTGCAAGCGTAA‑CCTG‑CAACGGCCCGTTGTCCGTCGCCAG‑CCAGAAGGAGACTTCTGTCCCTTGCGGGGTGTCCCGCCAGTGTC‑GGGTTAAGATAAAACCTGCCTGCGCCACGCTGAAAATCCATCAAAAAA‑CCA‑GGCTTGAGTATA‑G‑CCT‑GGTTTCGTTTGATTGGCTGTGGTTTtat                                                              <  2:344693/239‑1 (MQ=255)

                                                                                                                                                                                gCAAAATATGCTGGGGGGGGGGAACCTGATCGGCGGGAATAACCTCCGCGGACTCTGGCGGTGCAAGCGTAA‑CCTG‑CAACGGCCCGTGGTCCGTCGCCAG‑CCAGAAGGAGACTTCTGTCCCTTGCGGGGTGTCCCGCCAGTGTC‑GGGTTAAGATAAAACCTGCCTGCGCCACGCTGAAAAGCCATCAAAAAA‑CCA‑GGCTTGAGTATA‑G‑CCT‑GGTTTCGTTTGATTGGCTGTGGTTTtat                                                              <  2:395509/239‑1 (MQ=255)

                                                                                                                                                                                gCAAAATATGCTGAGCGCGGGGAACCTGATCGGCGGGAATAAACGCCACGGACTCTTGCGGTGCAAGCGTAA‑CCTG‑CAACGGCCCGTTGTCCGTCGCCAG‑CCAGAAGGAGACTTCTGTCCCTTGCGGGGTGTCCCGCCAGTGTC‑GGGTTAAGATAAAACCTGCCTGCGCCACGCTGAAAATCCATCAAAAAA‑CCA‑GGCTTGAGTATA‑G‑CCT‑GGTTTCGTTTGATTGGCTGTGGTTTtat                                                              <  1:926760/239‑1 (MQ=255)

                                                                                                                                                                                gCAAAATATGCTGAGCGCGGGGAACCTGATCGGCGGGAATAAACGCCACGGACTCTTGCGGTGCAAGCGTAA‑CCTG‑CAACGGCCCGTTGTCCGTCGCCAG‑CCAGAAGGAGACTTCTGTCCCTTGCGGGGTGTCCCGCCAGTGTC‑GGGTTAAGATAAAACCTGCCTGCGCCACGCTGAAAATCCATCAAAAAA‑CCA‑GGCTTGAGTATA‑G‑CCT‑GGTTTCGTTTGATTGGCTGTGGTTTtat                                                              <  1:1225153/239‑1 (MQ=255)

                                                                                                                                                                                  aaaaTATGCTGAGCGCGGGGAACCTGATCGGCGGGAATAAACGCCCCGGACTCTTGCGGTGCAAGCGTAA‑CCTG‑CAACGGCCCGTTGTCCGTCGCCAG‑CCAGAAGGAGACTTCTGTCCCTTGCGGGGTGTCCCGCCAGTGTC‑GGGTTAAGATAAAACCTGCCTGCGCCACGCTGAAAATCCATCAAAAAA‑CCA‑GGCTTGAGTATA‑G‑CCT‑GGTTTCGTTTGATTGGCTGTGGTTTTATAc                                                            <  1:1009158/239‑1 (MQ=255)

                                                                                                                                                                                  aaaaTATGCTGAGCGCGGGGAACCTGATCGGCGGGAATAAACGCCACGGACTCTTGCGGTGCAAGCGTAA‑CCTG‑CAACGGCCCGTTGTCCGTCGCCAG‑CCAGAAGGAGACTTCTGTCCCTTGCGGGGTGTCCCGCCAGTGTC‑GGGTTAAGATAAAACCTGCCTGCGCCACGCTGAAAATCCATCAAAAAA‑CCA‑GGCTTGAGTATA‑G‑CCT‑GGTTTCGTTTGATTGGCTGTGGTTTTATAc                                                            <  1:275577/239‑1 (MQ=255)

                                                                                                                                                                                  aaaaTATGCTGAGCGCGGGGAACCTGATCGGCGGGAATAAACGCCACGGACTCTTGCGGTGCAAGCGTAA‑CCTG‑CAACGGCCCGTTGTCCGTCGCCAG‑CCAGAAGGAGACTTCTGTCCCTTGCGGGGTGTCCCGCCAGTGTC‑GGGTTAAGATAAAACCTGCCTGCGCCACGCTGAAAATCCATCAAAAAA‑CCA‑GGCTTGAGTATA‑G‑CCT‑GGTTTCGTTTGATTGGCTGTGGTTTTATAc                                                            <  1:1167058/239‑1 (MQ=255)

                                                                                                                                                                                  aaaaTATGCTGAGCGCGGGGAACCTGACCGGCGGGAATAAACGCCACGGACTCTTGCGGTGCAAGCGTAA‑CCTG‑CAACGGCCCGTTGTCCGTCGCCAG‑CCAGAAGGAGACTTCTGTCCCTTGCGGGGTGTCCCGCCAGTGTC‑GGGTTAAGATAAAACCTGCCTGCGCCACGCTGAAAATCCATCAAAAAA‑CCA‑GGCTTGAGTATA‑G‑CCT‑GGTTTCGTTTGATTGGCTGTGGTTTTATAc                                                            <  2:1314308/239‑1 (MQ=255)

                                                                                                                                                                                  aaaaGATGCTGAGCGCGGGGAACCTGATCGGCGGGAATAAACGCCACGGACTCTTGCGGTGCAAGCGTAA‑CCTG‑CAACGGCCCGTTGTCCGTCGCCAG‑CCAGAAGGAGACTTCTGTCCCTTGCGGGGTGTCCCGCCAGTGTC‑GGGTTAAGATAAAACCTGCCTGCGCCACGCTGAAAATCCATCAAAAAA‑CCA‑GGCTTGAGTATA‑G‑CCT‑GGTTTCGTTTGATTGGCTGTGGTTTTATAc                                                            <  2:282793/239‑1 (MQ=255)

                                                                                                                                                                                     atatGCTGAGCGCGGGGAGCCTGATCGGCGGGAATAAACGCCACGGACTCTTGCGGTGCAAGCGTAA‑CCTG‑CAACGGCCCGTTGTCCGTCGCCAG‑CCAGAAGGAGACTTCTGTCCCTGGCGGGGTGTCCCGCCAGTGTC‑GGGTTAAGATAAAACCTGCCTGCGCCACGCTGAAAATCCATCAAAAAA‑CCA‑GGCTTGAGTATA‑G‑CCT‑GGTTTCGTTTGATTGGCTGTGGTTTTATACAGt                                                         <  1:1210888/239‑1 (MQ=255)

                                                                                                                                                                                     atatGCAGAGCGCGGGGAACCTGATCGGCGGGAATAACCGCCACGGCCTCTTGCGGTGCAAGCGTAA‑CCTG‑CAACGGCCCGTTGTCCGTCGCCAG‑CCAGAAGGAGACTTCTGTCCCTTGCGGGGTGTCCCGCCAGTGTC‑GGGTTAAGATAAAACCTGCCTGCGCCACGCTGAAAATCCATCAAAAAA‑CCA‑GGCTTGAGTATA‑G‑CCT‑GGTTTCGTTTGATTGGCTGTGGTTTTATACAGt                                                         <  1:930373/239‑1 (MQ=255)

                                                                                                                                                                                       atGCTGAGCGCGGGGAACCTGATCGGCGGGAATAAACGCCACGGACTCTTGCGGTGCAAGCGTAA‑CCTG‑CAACGGCCCGTTGTCCGTCGCCAG‑CCAGAAGGAGACTTCTGTCCCTTGCGGGGTGTCCCGCCAGTGTC‑GGGGTAAGATAAAAACTGCCTGCGCCACGCTGAAAATCCATCAAAAAA‑ACA‑GGCTTGAGGATT‑G‑CCC‑GGTTTCGTTTGATTGGGTGTGGTTTTTTACAGTCa                                                       >  1:28056/1‑239 (MQ=255)

                                                                                                                                                                                        tGCTGAGCGCGGGGAACCTGATCGGCGGGAATAAACGCCACGGACTCTTGCGGTGCAAGCGTAA‑CCTG‑CAACGGCCCGTTGTCCGTCGCCAG‑CCAGAAGGAGACTTCTGTCCCTTGCGGGGTGTCCCGCCAGTGTC‑GGGTTAAGATAAAACCTGCCTGCGCCACGCTGAAAATCCATCAAAAAA‑CCA‑GGCTTGAGTATA‑G‑CCT‑GGTTTCGTTTGATTGGCTGTGGTTTTATAc                                                            <  1:1069545/233‑1 (MQ=255)

                                                                                                                                                                                         gCTGAGCGCGGGGAACCTGATCGGCGGGAATAAACGCCACGGACTCTTGCGGTGCAAGCGTAA‑CCTG‑CCACGGCCCGTTGTCCGTCGCCCG‑CCAGAAGGAGACTTCTGTCCCTTGCGGGGGGTCCCGCCAGTGGC‑GGGGTAAAATAAAAACTGCCCGCGCCCCGCTGAAAAACCATCAAAAAA‑ACA‑CGGTTGAGGATA‑A‑CCT‑CGTTTTGTTTGTTTGGGGGGGGTTTTTTACAgcactt                                                     >  2:919037/1‑234 (MQ=255)

                                                                                                                                                                                           tGAGCGCGGGGAACCTGATCGGCGGGAATAAACGCCACGGACTCTTTCGGTGCAAGCGTAA‑CCCC‑CAACGGCCCGTTGTCCGTCCCCAG‑CCAGAAGGAGACTTCTGTCCCTTGCGGGGGGGCCCCCCCGTGTC‑CGGGTTATATCAAACCTCCCCTCGCCCCCCTGAAAATCCATCAAAAAA‑CCC‑CGCCTGAGGGTT‑G‑CCTCGGTTCGGTTTATTTGTCAGTGTTTTTTTACATTcttat                                                    >  2:20053/1‑235 (MQ=255)

                                                                                                                                                                                           tGAGCGCGGGGAACCTGATCGGCGGGAATAAACGCCACGGACTCTTGCGGTGCAAGCGTAA‑CCTG‑CAACGGCCCGTTGTCCGTCGCCAG‑CCAGAAGGAGACTTCTGTCCCTTGCGGGGTGTCCCGCCAGTGTC‑GGGTTAAGATAAAACCTGCCTGCGCCACGCTGAAAATCCATCAAAAAA‑CCA‑GGCTTGAGTATA‑G‑CCT‑GGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTa                                                    <  1:597539/238‑1 (MQ=255)

                                                                                                                                                                                            gAGCGCGGGGAACCTGATCGGCGGGAATAAACGCCACGGACTCTTGCGGTGCAAGCGTAA‑CCTG‑CAACGGCCCGTTGTCCGTCGCCAG‑CCAGAAGGAGACTTCTGTCCCTTGCGGGGTGTCCCGCCAGTGTC‑GGGTTAAGATAAAACCTGCCTGCGCCACGCTGAAAATCCATCAAAAAA‑CCA‑GGCTTGAGTATA‑G‑CCT‑GGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACt                                                  <  1:1135480/239‑1 (MQ=255)

                                                                                                                                                                                              gcgcGGGGAACCTGATCGGCGGGAATAAACGCCACGGACTCTTGCGGGGCAAGCGTAA‑CCTG‑CAACGGCCCGTTGTCCGTCGCCAG‑CCAGAAGGAGACTTCTGTCCCTTGCGGGGGGTCCCGCCCGTGTC‑GGGGTAAGATAAAACCTGCCTGCGCCACGCTGAAAATCCCTCAAAAAA‑ACA‑GGCTTGAGTATA‑G‑CCT‑GGTTTCGTTTGATTGGGTGTGGGTTTTTAAAGTCAtttatt                                                 >  2:550714/1‑234 (MQ=255)

                                                                                                                                                                                                gcgGGGAACCTGTCCGGAGGAAATAAACGCCACGGACTCTTGCGGTGCAAGCTTAC‑CCTG‑CAACGGCCCGTGGTCCGTCGCCAG‑CCAGAATGAGACTTCTTTCCCTTGCGGGGTGTCCCGCCAGTGTC‑GGGTTAAGATAAAACCTGCCTGCGCCACGCTGAAAATCCATCAAAAAA‑CCA‑GGCTTGAGTATA‑G‑CCT‑GGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGcc                                               <  2:378/238‑1 (MQ=255)

                                                                                                                                                                                                gcgGGGAACCTGATCGGCGGGAATAAACGCCACGGACTCTTGCGGTGCAAGCGTAA‑CCTG‑CAACGGCCCGTTGTCCGTCGCCAG‑CCAGAAGGAGACTTCTGTCCCTTGCGGGGTGTCCCGCCAGTGTC‑GGGTTAAGATAAAACCTGCCTGCGCCACGCTGAAAATCCATCAAAAAA‑CCA‑GGCTTGAGTATA‑G‑CCT‑GGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGcc                                               <  1:387394/238‑1 (MQ=255)

                                                                                                                                                                                                  ggggAACCTGATCGGCGGGAATAAACGCCACGGGCTCTTTCGGGGCAAGCGTAA‑CCTG‑CAACGGCCCGTTGTCCGTCGCCAG‑CCCGAAGGAGACCTCTGTCCCTTGCGGGGGGTTCCGCCAGTGGC‑GGGGTAAGATAAAACCTGCCCTCCCCCCGCCGAAAAATCACCCAAAAA‑CCAGGGTTTGGTTAAT‑G‑CCG‑GGTTTGGTTTTATTGGTGGGGGTTTTTTACATGCATTCCtcttgt                                             >  2:1069545/1‑234 (MQ=255)

                                                                                                                                                                                                  ggggAACCTGATCGGCGGGAATAAACGCCACGGACTCTTGCGGTGCAAGCGTAA‑CCTG‑CAACGGCCCGTTGTCCGTCGCCAG‑CCAGAAGGAGACTTCTGTCCCTTGCGGGGTGTCCCGCCAGTGTC‑GGGTTAAGATAAAACCTGCCTGCGCCACGCTGAAAATCCATCAAAAAA‑CCA‑GGCTTGAGTATA‑G‑CCT‑GGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGcc                                               <  1:535448/236‑1 (MQ=255)

                                                                                                                                                                                                         cTGATCGGCGGGAATAAACGCCACGGACTCTTGCGGTGCAAGCGTAA‑CCTG‑CAACGGCCCGTTGTCCGTCGCCAG‑CCAGAAGGAGACTTCTGTCCCTTGCGGGGTGTCCCGCCAGTGTC‑GGGTTAAGATAAAACCTGCCTGCGCCACGCTGAAAATCCATCAAAAAA‑CCA‑GGCt                                                                                                         <  1:417810/174‑1 (MQ=255)

                                                                                                                                                                                                           gATCGGCGGGAATAAACGCCACGGACTCTTGCGGGGCAAGCGTAA‑CCTG‑CACCGGCCCGTTGTCCGTCGCCAG‑CCAGAAGGAGACTTCTGTCCCTTGCGGGGTGTCCCGCCAGTGTC‑GGGTTAAGATAAAACCTGCCTGCGCCACGCTGAAAATCCATCAAAAAA‑CCA‑GGCTTGAGTATA‑G‑CCT‑GGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCt                                   <  2:452020/239‑1 (MQ=255)

                                                                                                                                                                                                                  gggAATAAACGCCACGGACTCTTGCGGTGCAAGCGTAA‑CCTG‑CAACGGCCCGTTGTCCGTCGCCAG‑CCAGAAGGAGACTTCTGTCCCTTGCGGGGTGTCCCGCCAGTGTC‑GGGTTAAGATAAAACCTGCCTGCGCCACGCTGAAAATCCATCAAAAAA‑CCA‑GGCTTGAGTATA‑G‑CCT‑GGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCTTCgcgc                             <  1:1072640/238‑1 (MQ=255)

                                                                                                                                                                                                                  gggAATAAACGCCACGGACTCTTGCGGTGCAAGCGTAA‑CCTG‑CAACGGCCCGTGGCCCGCCGCCAG‑CCAGAAGGAGACTTCTGTCCCTTGCGGGGTGTCCCGCCAGTGTC‑GGGTTAAGATAAAACCTGCCTGCGCCACGCTGAAAATCCATCAAAAAA‑CCA‑GGCTTGAGTATA‑G‑CCT‑GGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCTTCgcgc                             <  2:363327/238‑1 (MQ=255)

                                                                                                                                                                                                                       tAAACGCCACGGACTCTTGCGGTGCAAGCGTAA‑CCTG‑CAACGGCCCGTGGTCCGTCGCCAG‑CAAGAAGGAGACTTCTGCCCCTTGCGGGGTGTCCCGCCAGTGTC‑GGGTTAAGATAAAACCTGCCTGCGCCACGCTGAAAATCCATCAAAAAA‑CCA‑GGCTTGAGTATA‑G‑CCT‑GGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCTTCGCGCCATGCt                       <  1:926031/239‑1 (MQ=255)

                                                                                                                                                                                                                               aCGGACTCTTGCGGTGCAAGCGTAG‑CCTG‑CAACGGCCCGTTGTCCGTCGCCAG‑CCAGAAGGAGACTTCTGTCCCTTGCGGGGTGTCCCGCCAGTGTC‑GGGTTAAGATAAAACCTGCCTGCGCCACGCTGAAAATCCATCAAAAAA‑CCA‑GGCTTGAGTATA‑G‑CCT‑GGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCTTCGCGCCATGCTTACGCAGa               <  1:662599/239‑1 (MQ=255)

                                                                                                                                                                                                                               aCGGACTCTTGCGGTGCAAGCGTAA‑CCTG‑CAACGGCCCGTTGTCCGTCGCCAG‑CCAGAAGGAGACTTCTGTCCCTTGCGGGGTGTCCCGCCAGTGTC‑GGGTTAAGATAAAACCTGCCTGCGCCACGCTGAAAATCCATCAAAAAA‑CCA‑GGCTTGAGTATA‑G‑CCT‑GGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATTCCTTCCCGCCCTGCTTACGCAgt               >  2:174171/1‑238 (MQ=255)

                                                                                                                                                                                                                               aCGGACTCTTGCGGTGCAAGCGTAA‑CCTG‑CAACGGCCCGTTGTCCGTCGCCAG‑CCAGAAGGAGACTTCTGTCCCTTGCGGGGTGTCCCGCCAGTGTC‑GGGTTAAGATAAAACCTGCCTGCGCCACGCTGAAAATCCATCAAAAAA‑CCA‑GGCTTGAGTATA‑G‑CCT‑GGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCTTCGCGCCATGCTTACGCAGa               <  1:174171/239‑1 (MQ=255)

                                                                                                                                                                                                                                cGGACTCTTGCGGTGCAAGCGTAA‑CCTG‑CAACGGCCCGTTGTCCGTCGCCAG‑CCAGAAGGAGACTTCTGTCCCTTGCGGGGTGTCCCGCGAGTGTC‑GGGTTAAGATAAAACCTGCCTGCGCCACGCTGAAAATCCATCAAAAAA‑CCA‑GGCTTGAGTATA‑G‑CCT‑GGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCTTCGCGCCATGCTTACGCAGAt              <  1:711437/239‑1 (MQ=255)

                                                                                                                                                                                                                                cGGACTCTTGCGGTGCAAGCGTAA‑CCTG‑CAACGGCCCGTTGTCCGTCGCCAG‑CCAGAAGGAGACTTCTGTCCCTTGCGGGGTGTCCCGCCAGTGTC‑GGGTTAAGATAAAACCTGCCTGCGCCACGCTGAAAATCCATCAAAAAA‑CCA‑GGCTTGAGTATA‑G‑CCT‑GGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCTTCGCGCCATGCTTACGCAGAt              <  1:550714/239‑1 (MQ=255)

                                                                                                                                                                                                                                   aCTCTTGCGGTGCAAGCGTAA‑CCTG‑CAACGGCCCGTTGTCCGTCGCCAG‑CCAGAAGGAGACTTCTGTCCCTTGCGGGGTGTCCCGCCAGTGTC‑GGGTTAAGATAAAACCTGCCTGCGCCACGCTGAAAATCCATCAAAAAA‑CCA‑GGCTTGAGTATA‑G‑CCT‑GGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCTTCGCGCCATGCTTACGCAGATAgt           >  1:611669/1‑239 (MQ=255)

                                                                                                                                                                                                                                    ctctTGCGGTGCAAGCGTAA‑CCTG‑CAACGGCCCGTTGTCCGTCGCCAG‑CCAGAAGGAGACTTCTGTCCCTTGCGGGGTGTCCCGCCAGTGTC‑GGGTTAAGATAAAACCTGCCTGCGCCACGCTGAAAATCCATCAAAAAA‑CCA‑GGCTTGAGTATA‑G‑CCT‑GGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCTTCGCGCCATGCTTACGCAGATAgt           <  1:39819/238‑1 (MQ=255)

                                                                                                                                                                                                                                     tctcGCGGTACAAGCTTAA‑CCTG‑AAACGACCCGTTGTCCGTCGCCAG‑CCAGAAGGAGACTTCTGTCCCTTGGGGGGTGTCCCGCCAGTGTC‑GGGTTAAGATAAAACCTGCCTGCGCCACGCTGAAAATCCATCAAAAAA‑CCA‑GGCTTGAGTATA‑G‑CCT‑GGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCTTCGCGCCATGCTTACGCAGATAgtgt         <  1:563627/239‑1 (MQ=255)

                                                                                                                                                                                                                                     tctTGCGGTGCAAGCGTAA‑CCTG‑CAACGGCCCGTTGTCCGTCGCCAG‑CCAGAAGGAGACTTCTGTCCCTTGCGGGGTGTCCCGCCAGTGTC‑GGGTTAAGATAAAACCTGCCTGCGCCACGCTGAAAATCCATCAAAAAA‑CCA‑GGCTTGAGTATA‑G‑CCT‑GGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCTTCGCGCCATGCTTACGCAGATAgtgt         <  1:224483/239‑1 (MQ=255)

                                                                                                                                                                                                                                     tctTGCGGTGCAAGCGTAA‑CCTG‑CAACGGCCCGTTGTCCGTCGCCAG‑CCAGAAGGAGACTTCTGTCCCTTGCGGGGTGTCCCGCCAGTGTC‑GGGTTAAGATAAAACCTGCCTGCGCCACGCTGAAAATCCATCAAAAAA‑CCA‑GGCTTGAGTATA‑G‑CCT‑GGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCTTCGCGCCATGCTTACGCAGATAgtgt         <  1:1057315/239‑1 (MQ=255)

                                                                                                                                                                                                                                       ttGCGGTGCAAGCGTAA‑CCTG‑CAACGGCCCGTTGTCCGTCGCCAG‑CCAGAAGGAGACTTCTGTCCCTTGCGGGGTGTCCCGCCAGTGTC‑GGGTTAAGATAAAACCTGCCTGCGCCACGCTGAAAATCCATCAAAAAA‑CCA‑GGCTTGAGTATA‑G‑CCT‑GGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACt                                                  >  2:612718/1‑196 (MQ=255)

                                                                                                                                                                                                                                       ttGCGGTGCAAGCGTAA‑CCTG‑CAACGGCCCGTTGTCCGTCGCCAG‑CCAGAAGGAGACTTCTGTCCCTTGCGGGGTGTCCCGCCAGTGTC‑GGGTTAAGATAAAACCTGCCTGCGCCACGCTGAAAATCCATCAAAAAA‑CCA‑GGCTTGAGTATA‑G‑CCT‑GGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACt                                                  <  1:612718/196‑1 (MQ=255)

                                                                                                                                                                                                                                        tGCGGTGCAAGCGTAA‑CCTG‑CAACGGCCCGTTGTCCGTCGCCAG‑CCAGAAGGAGACTTCTGTCCCTTGCGGGGTGTCCCGCCAGTGTC‑GGGTTAAGATAAAACCTGCCTGCGCCACGCTGAAAATCCATCAAAAAA‑CCA‑GGCTTGAGTATA‑G‑CCT‑GGGTTCGTTTGATTGGCTGTGGATTTTTACAGTAATTACTGCCCGTAATATGCCTTCGCGCCATGCTTCAGCAGATAGTGtttt      >  2:1126867/1‑238 (MQ=255)

CATCAGATAGCGTTCTGGCGGACGCACATCGGCCTCGTAGACGGTAACGCCACCTTCACGCAGGCGCTTTTCGTAATTCATCAATTGGCGATGGGCGCGACAGTAAAGGCCATACACCGGCTGGCGGTGAAAATCCTTTAACGCCAGCGGTGTCAGGCGAAAGCCTTGTTCACCCTGCAAAATATGCTGAGCGCGGGGAACCTGATCGGCGGGAATAAACGCCACGGACTCTTGCGGTGCAAGCGTAA‑CCTG‑CAACGGCCCGTTGTCCGTCGCCAG‑CCAGAAGGAGACTTCTGTCCCTTGCGGGGTGTCCCGCCAGTGTC‑GGGTTAAGATAAAACCTGCCTGCGCCACGCTGAAAATCCATCAAAAAA‑CCA‑GGCTTGAGTATA‑G‑CCT‑GGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCTTCGCGCCATGCTTACGCAGATAGTGTTTATCCA  >  NC_000913/65433‑65907

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`