Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	989,901	G→T	17.0%	S152R (AGC→AGA)	pncB ←	nicotinate phosphoribosyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	989,901	0	G	T	17.0%	167.0 / 6.5	88	S152R (AGC→AGA)	pncB	nicotinate phosphoribosyltransferase
Reads supporting (aligned to +/- strand):  ref base G (25/44);  new base T (12/4);  total (38/50)
Fisher's exact test for biased strand distribution p-value = 1.01e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.52e-01

GGCTGATTTGCTGATGTGCCTGGAACCATTCGTGTGCCTGTGTTCCCATCGGCGTGAGGGAAAGCCGACGCGCCAGATCGTAGTTGCTGGTGCCCACAAACCAGGATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCG‑ACGGGTGCCAAAATCCA‑TCAGATGGAAGCGCGACATATCAAGACCGGC‑GGTTAAC‑GCCGAGAAG‑TCGAC‑TAATTTGCTTTCCAGCGTGTCGAGGGCTTGCG‑CAAC‑GTCGGCCTGCGGTGAGCGATAGCGATGTACCATTTCACTGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGAC‑TTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCTTAAAGAAAGGCAGGGCAGAAAGC  >  NC_000913/989666‑990138                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                 ggCTGATTTGCTGATGTGCCTGGAACCATTCGTGTGCCTGTGTTCCCATCGGCGTGAGGGAAAGCCGACGCGCCAGATCGTAGTTGCTGGTGCCCACAAACCAGGATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCG‑ACGGGTGCCAAAATCCA‑TCAGATGGAAGCGCGACATATCAAGACCGGC‑GGGTAAC‑GCCGGGAAG‑TCGGC‑GAATTTGCtt                                                                                                                                                                                                                                               >  2:173858/1‑239 (MQ=255)       tttGCTGATGTGCCTGGAACCATTCGTGTGCCTGTGTTCCCATCGGCGTGAGGGAAAGCCGACGCGCCAGATCGTAGTTGCTGGTGCCCACAAACCAGGATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCG‑ACGGGTGCCAAAATCCA‑TCAGATGGAAGCGCGACATATCAAGACCGGC‑GGTTAAC‑GCCGAGAAG‑TCGAC‑TAATTTGCTTTCCAGc                                                                                                                                                                                                                                         <  2:1354006/239‑1 (MQ=255)                gtgCCTGGAACCATTCGTGTGCCTGTGTTCCCATCGGCGTGAGGGAAAGCCGACGCGCCAGATCGTAGTTGCTGGTGCCCACAAACCAGGATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCG‑ACGGGTGCCAAAATCCA‑TCAGATGGAAGCGCGACATATCAAGACCGGCGGGTTAAC‑‑CCGCGGAA‑GCGACATAATTTG‑TTTCCAGCGTGTCGaag                                                                                                                                                                                                                                >  1:1311075/1‑237 (MQ=255)                  gCCTGGAACCATTCGTGTGCCTGTGTTCCCATCGGCGTGAGGGAAAGCCGACGCGCCAGATCGTAGTTGCTGGTGCCCACAAACCAGGATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCG‑ACGGGTGCCAAAATCCA‑TCAGATGGAAGCGCGACATATCAAGACCGGC‑GGTTAAC‑GCCGAGAAG‑TCGAC‑TAATTTGCTTTCCAGCGTGTCGAGGGc                                                                                                                                                                                                                              <  1:738982/239‑1 (MQ=255)                  gCCTGGAACCATTCGTGTGCCTGTGTTCCCATCGGCGTGAGGGAAAGCCGACGCGCCAGATCGTAGTTGCTGGTGCCCACAAACCAGGATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCG‑ACGGGTGCCAAAATCCA‑TCAGATGGAAGCGCGACATATCAAGACCGGC‑GGTTAAC‑GCCGAGAAG‑TCGAC‑TAATTTGCTTTCCAGCGTGTCGAGGGc                                                                                                                                                                                                                              <  1:659136/239‑1 (MQ=255)                  gCCTGGAACCATTCGTGTGCCTGTGTTCCCATCGGCGTGAGGGAAAGCCGACGCGCCAGATCGTAGTTGCTGGTGCCCACAAACCAGGATTACTGTTGCAGACGCTTAACGATGGTTTCTTGTGCTTCGCGAGAAAAACGGCG‑ACGGGTGCCAAAATCCA‑TCAGATGGAAGCGCGACATATCAAGACCGGC‑GGTTAAC‑GCCGAGAAG‑TCGAC‑TAATTTGCTTTCCAGCGTGTCGAGGGc                                                                                                                                                                                                                              <  2:1076533/239‑1 (MQ=255)                   ccTGGAACCATTCGTGTGCCTGTGTTCCCATCGGCGTGAGGGAAAGCCGACGCGCCAGATCGTAGTTGCTGGTGCCCACAAACCAGGATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCG‑ACGGGTGCCAAAATCCA‑TCAGATGGAAGCGCGACATATCAAGACCGGC‑GGTTAAC‑GCCGAGAAG‑TCGAC‑TAATTTGCTTTCCAGCGTGTCGAg                                                                                                                                                                                                                                 <  1:61450/235‑1 (MQ=255)                   ccTGGAACCATTCGTGTGCCTGTGTTCCCATCGGCGTGAGGGAAAGCCGACGCGCCAGATCGTAGTTGCTGGTGCCCACAAACCAGGATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCG‑ACGGGTGCCAAAATCCA‑TCAGATGGAAGCGCGACATATCAAGACCGGC‑GGGTAAA‑GCCGCGAAG‑TGGAC‑TAAATTTTTTTTCAGCGGGTCGAggggt                                                                                                                                                                                                                             >  1:587505/1‑237 (MQ=255)                        aaCCATTCGTGTGCCTGTGTTCCCATCGGCGTGAGGGAAAGCCGACGCGCCAGATCGTAGTTGCTGGTGCCCACAAACCAGGATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCG‑ACGGGTGCCAAAATCCA‑TCAGATGGAAGCGCGACATATCAAGACCGGC‑GGGTAAA‑GACGAGAAG‑GCGAC‑TCAATTGCTTTTCAGCGTGTTGGGGGCCTgcg                                                                                                                                                                                                                         >  1:731928/1‑238 (MQ=255)                             ttCGTGTGCCTGTGTTCCCATCGGCGTGAGGGAAAGCCGACGCGCCAGATCGTAGTTGCTGGTGCCCACAAACCAGGATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCG‑ACGGGTGCCAAAATCCA‑TCAGATGGAAGCGCGACATATCAAGACCGGC‑GGTTTAC‑GCCGTAAAG‑GAGGC‑GAATTTGCTTTCCTGCCGGTGTAGGGCGTgccggcag                                                                                                                                                                                                                    >  2:998206/1‑232 (MQ=255)                             ttCGTGTGCCTGTGTTCCCATCGGCGTGAGGGAAAGCCGACGCGCCAGATCGTAGTTGCTGGTGCCCACAAACCAGGATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCG‑ACGGGTGCCAAAATCCA‑TCAGATGGAAGCGCGACATATCAAGACCGGC‑GGTTAAA‑GCCCAGAAG‑TCGAC‑TAATTTTCTTTTCACCGTGTCGAGGGGTTGCG‑CCAC‑g                                                                                                                                                                                                                  >  2:1175243/1‑238 (MQ=255)                             ttCGTGTGCCTGTGTTCCCATCGGCGTGAGGGAAAGCCGACGCGCCAGATCGTAGTTGCTGGTGCCCACAAACCAGGATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCG‑ACGGGTGCCAAAATCCA‑TCAGATGGAAGCGCGACATATCAAGACCGGC‑GGGTTAA‑GCCGCGAAG‑GCGGC‑TAATATGCTTTTCCCCCTGTCGAGGGACTGCG‑CGActg                                                                                                                                                                                                                  >  2:61450/1‑237 (MQ=255)                                     ccTGTGTTCCCATCGGCGTGAGGGAAAGCCGACGCGCCAGATCGTAGTTGCTGGTGCCCACAAACCAGGATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCG‑ACGGGTGCCAAAATCCA‑TCAGATGGAAGCGCGACATATCAAGACCGGC‑GGGTAAA‑ACCGCGAAG‑ACGGC‑ACAATTTCTTTTCAGCGGGGTGTGGGCGTGCG‑GAAC‑GCCgggccgc                                                                                                                                                                                                         >  2:557080/1‑234 (MQ=255)                                      cTGTGTTCCCATCGGCGTGAGGGAAAGCCGACGCGCCAGATCGTAGTTGCTGGTGCCCACAAACCAGGATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCG‑ACGGGTGCCAAAATCCA‑TCAGATGGAAGCGCGACATATCAAGACCGGC‑GGTTAAC‑GCCGAGAAG‑TCGAC‑TAATTTGCTTTCCAGCGTGTCGAGGGCTTGCG‑CAAC‑GTCGGCCTGCg                                                                                                                                                                                                        <  2:150905/239‑1 (MQ=255)                                      cTGTGTTCCCATCGGCGTGAGGGAAAGCCGACGCGCCAGATCGTAGTTGCTGGTGCCCACAAACCAGGATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCG‑ACGGGTGCCAAAAGCCA‑TCAGATGGAAGCGCGACATATCAAGACCGGC‑GGTTAAC‑GCCGAGAAG‑TCGAC‑TAATTTGCTTTCCAGCGTGTCGAGGGCTTGCG‑CAAC‑GTCGGCCTGCg                                                                                                                                                                                                        <  2:374055/239‑1 (MQ=255)                                      cTGTGTTCCCATCAGCGTGAGGGAAAGCCGACGCGCCAGTTAGTAGTGGCTGGGTCCTTCAAACCAGGATTCCTGTTGCAGACGGTTAACGATGGTTTCTTGTACTGCCCGAGACAGACGGCT‑ACGGGTGCCAAAATCCA‑TCAGATGGAAGCGCTACATATCAAGACCGGC‑GGTTAAC‑TCTGAGAAG‑TCGAC‑TAATTTTTTTTCCTGCGTGTCGAGGGCCTGCG‑CATA‑GTCGGCTTGCg                                                                                                                                                                                                        <  2:1037453/239‑1 (MQ=255)                                        gtgtTCCCATCGGCGTGAGGGAAAGCCGACGCGCCAGATCGTAGTTGCTGGTGCCCACAAACCAGGATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCG‑ACGGGTGCCAAAATCCA‑TCAGATGGAAGCGCGACATATCAAGACCGGC‑GGGTAAC‑GCCGAGAAG‑ACGAC‑TAATTTGCTTTTCAGCGTGTCGGGGGGTTGCG‑CAAC‑GTCCGCCTGCGGt                                                                                                                                                                                                      >  1:139925/1‑239 (MQ=255)                                         tgtTCCCATCGGCGTGAGGGAAAGCCGACGCGCCAGATCGTAGTTGCTGGTGCCCACAAACCAGGATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCG‑ACGGGTGCCAAAATCCA‑TCAGATGGAAGCGCGACATATCAAGACCGGC‑GGGTAAC‑GCCGAGAAGATGGGC‑AAATTT‑CTTTTCAGCGTGGCGAGGGCCTGCG‑CAAC‑CCCGGCCCGCgggg                                                                                                                                                                                                     >  1:1009214/1‑237 (MQ=255)                                          gtTCCCATCGGCGTGAGGGAAAGCCGACGCGCCAGATCGTAGTTGCTGGTGCCCACAAACCAGGATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCG‑ACGGGTGCCAAAATCCA‑TCAGATGGAAGCGCGACATATCAAGACCGGC‑GGTTAAC‑GCCGAGAAG‑TCGAC‑TAATTTGCTTTCCAGCGTGTCGAGGGCTTGCG‑CAAC‑GTCGGCCTGCGGTg                                                                                                                                                                                                     <  2:366970/238‑1 (MQ=255)                                              cctccGGCGTGAGGGAAAGCCGCCGCGCCAGATCGTAGTTTCTGGTTCCCACAAACCAGGATTCCTGTAGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCG‑ACGGGTGCCAAAATCCA‑TCAGATGGAAGCGCGACATATCAAGACCGGC‑GGTTAAC‑GCCGAGAAG‑TCGAC‑TAATTTGCTTTCCAGCGTGTCGAGGGCTTGCG‑CAAC‑GTCGGCCTGCGGTGagcga                                                                                                                                                                                                <  2:392466/235‑1 (MQ=255)                                              ccATCGGCGTGAGGGAAAGCCGACGCGCCAGATCGTAGTTGCTGGTGCCCACAAACCAGGATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCG‑ACGGGTGCCAAAATCCA‑TCAGATGGAAGCGCGACATATCAAGACCGGC‑GGTTAAC‑GCCGAGAAG‑TCGAC‑TAATTTGCTTTCCAGCGTGTCGAGGGCTTGCG‑CAAC‑GTCGGCCTGCGGTGagcga                                                                                                                                                                                                <  1:106280/239‑1 (MQ=255)                                              ccATCGGCGTGAGGGAAAGCCGACGCGCCAGATCGTAGTTGCTGGTGCCCACAAACCAGGATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCG‑ACGGGTGCCAAAATCCA‑TCAGATGGAAGCGCGACATATCAAGACCGGC‑GGTTAAC‑GCCGAGAAG‑TCGAC‑TAATTTGCTTTCCAGCGTGTCGAGGGCTTGCG‑CAAC‑GTCGGCCTGCGGTGagcga                                                                                                                                                                                                <  2:1349062/239‑1 (MQ=255)                                              ccATCGGCGTGAGGGAAAGCCGACGCGCCAGATCGTAGTTGCTGGTGCCCACAAACCAGGATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCG‑ACGGGTGCCAAAATCCA‑TCAGATGGAAGCGCGACATATCAAGACCGGC‑GGTTAAC‑GCCGAGAAG‑TCGAC‑TAATTTGCTTTCCAGCGTGTCGAGGGCTTGCG‑CAAC‑GTCGGCCTGCGGTGagcga                                                                                                                                                                                                <  2:848897/239‑1 (MQ=255)                                                aTCGGCGTGAGGGAAAGCCGACGCGCCAGATCGTAGTTGCTGGTGCCCACAAACCAGGATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCG‑ACGGGTGCCAAAATCCA‑TCAGATGGAAGCGCGACATATCAAGACCGGC‑GGTTTAA‑GCCGAGAAG‑TCGGC‑TAATTTGCTTTTCCGCGTGTCGGGGGGTTTGG‑CCAC‑GTCGGCCCGCGGGGaggggta                                                                                                                                                                                              >  1:386035/1‑234 (MQ=255)                                                aTCGGCGTGAGGGAAAGCCGACGCGCCAGATCGTAGTTGCTGGTGCCCACAAACCAGGATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCG‑ACGGGTGCCAAAATCCA‑TCAGATGGAAGCGCGACATATCAAGACCGGC‑GGTTAAC‑GCCGGGAAG‑ACGAC‑CAATTTGCTTTTCAGCGCGTCGAGGGGTTTCG‑CAAC‑GTCGGCCGCCGGTGggcgata                                                                                                                                                                                              >  1:884048/1‑239 (MQ=255)                                                     cGTGAGGGAAAGCCGACGCGCCAGATCGTAGTTGCTGGTGCCCACAAACCAGGATTCCTGTTGCAGACGCTTAACGCTGGTTTCTTGTACTTCGCGAGAAAAACGGCG‑ACGGGTGCCAAAATCCA‑TCAGATGGAAGCGCGACATATCAAGACCGGC‑GGTTAAC‑GCCGCGAAG‑TCGGA‑CAATTTTTTTTTCAGCGTGTGCGGGGCTTGCG‑CAAC‑ATCCGCCTGCGGTGggcggtatcgat                                                                                                                                                                                         >  1:531185/1‑239 (MQ=255)                                                     cGTGAGGGAAAGCCGACGCGCCAGATCGTAGTTGCTGGTGCCCACAAACCAGGATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCG‑ACGGGTGCCAAAATCCA‑TCAGATGGAAGCGCGACATATCAAGACCGGC‑GGTTAAC‑GCCGAGAAG‑TCGAC‑TAATTTGCTTTCCAGCGTGTCGAGGGCTTGCG‑CAAC‑GTCGGCCTGCGGTGagcgatagcga                                                                                                                                                                                          <  1:1268279/238‑1 (MQ=255)                                                      gTGAGGGAAAGCCGACGCGCCAGATCGTAGTTGCTGGTGCCCACAAACCAGGATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCG‑ACGGGTGCCAAAATCCA‑TCAGATGGAAGCGCGACATATCAAGACCGGC‑GGTTAAC‑GCCGAGAAG‑TCGAC‑TAATTTGCTTTCCAGCGTGTCGAGGGCTTGCG‑CAAC‑GTCGGCCTGCGGTGAGCGATAGCGATg                                                                                                                                                                                        <  1:660447/239‑1 (MQ=255)                                                      gTGAGGGAAAGCCGACGCGCCAGATCGTAGTTGCTGGTGCCCACAAACCAGGATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCG‑ACGGGTGCCAAAATCCA‑TCAGATGGAAGCGCGACATATCAAGACCGGC‑GGTTAAC‑GCCGAGAAG‑TCGAC‑TAATTTGCTTTCCAGCGTGTCGAGGGCTTGCG‑CAAC‑GTCGGCCTGCGGTGAGCGATAGCGATg                                                                                                                                                                                        <  2:1184633/239‑1 (MQ=255)                                                       tGAGGGAAAGCCGACGCGCCAGATCGTAGTTGCTGGTGCCCACAAACCAGGATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCG‑ACGGGTGCCAAAATCCA‑TCAGATGGAAGCGCGACATATCAAGACCGGC‑GGTTAAC‑GCCCCGAAG‑TTGAC‑CAATTTTCTTTTCAGCGTGTCGCGGGCTTGCG‑CAAC‑CCCGGCGCGCGGTGggggatagcgtagt                                                                                                                                                                                       >  1:261511/1‑235 (MQ=255)                                                            gAAAGCCGACGCGCCAGATCGTAGTTGCTGGTGCCCACAAACCAGGATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCG‑ACGGGTGCCAAAATCCA‑TCAGATGGAAGCGCGACATATCAAGACCGGC‑GGTTAAC‑GCCGAGAAG‑TCGAC‑TAATTTGCTTTCCAGCGTGTCGAGGGCTTGCG‑CAAC‑GTCGGCCTGCGGTGAGCGATAGCGATGTACCa                                                                                                                                                                                   <  1:988885/238‑1 (MQ=255)                                                                                  aGTTGCTGGTGCCCACAAACCAGGATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCG‑ACGGGTGCCAAAATCCA‑TCAGATGGAAGCGCGACATATCAAGACCGGC‑GGTTAAC‑GCCGCGAAG‑TCGAC‑TAATTTGCTTTTCAGCGGGTCGAGGGCTTGCG‑CAAC‑GTCGGCCTGCGGGGAGCGATAGCGATGTACCATTTCTCTGCTAAACGCCCGCaaa                                                                                                                                                            >  2:1103907/1‑239 (MQ=255)                                                                                  aGTTGCTGGTGCCCACAAACCAGGATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCG‑ACGGGTGCCAAAATCCA‑TCAGATGGAAGCGCGACATATCAAGACCGGC‑GGTTAAC‑GCCGAGAAG‑TCGAC‑TAATTTGCTTTCCAGCGTGTCGAGGGCTTgcg                                                                                                                                                                                                                         <  1:1231273/180‑1 (MQ=255)                                                                                         ggTGCCCACAAACCAGGATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCG‑ACGGGTGCCAAAATCCA‑TCAGATGGAAGCGCGACATATCAAGACCGGC‑GGTTTAC‑CACGAGAAG‑GACAA‑TAATTTGCTTTCCAGCGTGCCGCAGGCTTGCG‑CCAC‑GCCGCCCCGCGGTGTGCGGTAGCGGTGTCACATTTTACTGATAACCGCCAGCaacgacact                                                                                                                                                      >  2:7315/1‑231 (MQ=255)                                                                                            gCCCACAACCCAGGATTCCTGTGGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAAAAAAACGGCG‑ACGGGTGCCAAAATCCA‑TCAGATGGAAGCGCGACATATCAAGACCGGC‑GGTTAAC‑GCCGAGAAG‑TCGAC‑TAATTTGCTTTCCAGCGTGTCGAGGGCTTGCG‑CAAC‑GTCGGCCTGCGGTGAGCGATAGCGATGTACCATTTCACTGATAACCGCCAGCAAAGGAACTTcc                                                                                                                                                   <  1:499147/238‑1 (MQ=255)                                                                                            gCCAACAAACCAGGATCCCGGTGGAAGCCGCTTAACGATGTTTTCTTGTACTTCGCGAGAAAAACGGCG‑ACGGGTGCCAAAATCCA‑TCAGATGGAAGCGCGAAATATCAAGCCCGGC‑GGTTAAC‑GCCGAGAAG‑TCGAC‑TAATTTGCTTCCCAGCGTGTCGAGGGCTTGCG‑CAAC‑GTCGGCCTGCGGTGAGCGATAGCGATGTACCATTTCACTGATAACCGCCAGCAAAGGAACTTcc                                                                                                                                                   <  2:587505/238‑1 (MQ=255)                                                                                             cccACAAACCAGGTTTCCTGTTGAGGACGCTTAAGGATGTTTTCTTGTACTTCGCGAGAAAAACGGCG‑AGGGGGGCAAAAATCCA‑TCAGAGGGAAGCGCGACATATCAAGACCGGC‑GGTTAAC‑GCGGAGAAG‑TCGAC‑TAATTTGCTTTCCAGCGTGTCGAGGGCTTGCG‑AAAC‑GTCGGCCGGGGGTGAGCGATAGCGATGTACCATTTCACGGATAACCGCCAGCAAAGGAACTTCCCa                                                                                                                                                 <  2:238203/239‑1 (MQ=255)                                                                                                            tCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCG‑ACGGGTGCCAAAATCCA‑TCAGATGGAAGCGCGACATATCAAGACCGGC‑GGTTAAC‑GCCGAGAAG‑TCGAC‑TAATTTTCTTTCCAGCGTGTCGCGGGCTTGCG‑CAAC‑GTCGGCCCGCGGGGAGCGATAGCGATGTACCCTTTTCCTGTCAACCGCCAGGAAAGGAAATTCCCCGAGGAGGACTTCAc                                                                                                                                   >  2:908378/1‑238 (MQ=255)                                                                                                            tCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCG‑ACGGGTGCCAAAATCCA‑TCAGATGGAAGCGCGACATATCAAGACCGGC‑GGTTAAA‑GCCGCGAAG‑GCGAA‑CAATTTTCTTTTCAGGGTGTCGAGGGCTTTCG‑CCAC‑GTCGGGCTGCGGGGGGCGGTAGCGATGTACCCTTTCACTGATAAACGCCAGCAAAGGAAATTCCCCGAAGAAGACtttcc                                                                                                                                   >  2:1191028/1‑235 (MQ=255)                                                                                                                  tGCAGCCGCTTAACGATGGTTTCTTGTCCTTCGCGAAAAAAACGGCG‑AGGGGTGCCAAAATCCA‑TAAGAGGGAGGCGCACCATAACAAGCCGGGC‑GGTTACC‑GCGGAGAAG‑TCGAT‑TAATTTGCTTCCCGGCGTGTCGAGGGTTTGCG‑CAAC‑GTCGGCCTGGGGTGAGCGATAGCGATGTACCATTTCACTGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACg                                                                                                                             <  2:26679/238‑1 (MQ=255)                                                                                                                  tGCAGACGCTTAACGATGTTTTCTTGTACTGCGAAAAAAAAACGGCG‑CCGGGTGCCAAAACCGA‑TCAGATGGAAGCGGGACATATCAAGCCCGGC‑GGTTAAC‑GCCGAGACG‑TCGAC‑TATTTTGCTTCCGAGCGTGTCGAGGGCTTGCG‑CAAC‑GTCGGCCTGCGGTGGGCGATAGCGATGTCCCATTTCACGGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACgg                                                                                                                            <  2:82009/239‑1 (MQ=255)                                                                                                                   gCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCG‑ACGGGTGCCAAAATCCA‑TCAGATGGAAGCGCGACATACCAAGACCGGC‑GGTTAAA‑GCCCAGAAG‑TCGAC‑CAATTTGTTTTCCAGCGGGTTGGGGGGTTGCGACAAC‑‑TCGGCCTCCGGGGGGCGATAGCAGTGTGCCATTTTCCTGGTAAACGCCAGCAAAGGAACCTCCCAGAGGACGGCTTCCcaccactg                                                                                                                            >  2:363273/1‑236 (MQ=255)                                                                                                                         gataacggttGGTTTTTTGATCTTCG‑GAAAAAAACCGTGCACGGGTGCAAAAATCCA‑TAAGATGAAGTCGCAAAATAGCAAGACGGGC‑‑GTTAACGGCCGAGAGG‑CCGAC‑TATTTGGCTTTCCAGCGTGTCAGGGGCTTGCG‑AATC‑GTCGGCCTGCGGTGGGCATTGGCAATGTCCCTTTCCCCTGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCtt                                                                                                                     <  2:1125025/230‑1 (MQ=255)                                                                                                                             aaCGATGGTTTTTTGTACTTGGAGAAAAAAACGGCG‑AGGGGTGCCAAAATCCA‑TCAGATGGAAGCGCGACATATCAAGCCCGGC‑GGTTAAC‑GCCGAGAAG‑TCGAC‑TAATTTGCTTTCCAGCGTGTGGAGGGCTTGCG‑AAAC‑GTCGGCCTGGGGTGAGCGATAGCGATGTCCCTTTTCACTGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAAc                                                                                                                 <  1:557080/239‑1 (MQ=255)                                                                                                                                   ggTTTCTTGTACTTCGCGAGAAAAACGGCG‑ACGGGTGCCAAAATCCA‑TCAGATGGAAGCGCGACATATCAAGACCGGC‑GGTTAAC‑GCCGGGAAG‑TCGAC‑TAATTTGCTTTCCAGCGTGTCGAGGGCTTGCG‑CAAA‑GGCGGCCTGCGGTGAGCGGTAGCGATGTACCATTTTACTGATAACCGCCAGCAAAGGAACTTCCCCGAGGATGACTTCACGCCCCGGGCCGCTTAAACGAata                                                                                                            >  1:641121/1‑238 (MQ=255)                                                                                                                                   ggTTTCTTGTACTTCGCGAGAAAAACGGCG‑ACGGGTGCCAAAATCCA‑TCAGATGGAAGCGCGACATATCAAGACCGGC‑GGTTAAC‑GCCGAGAAA‑ACGAC‑TAAATTGCCTTTCAACCGGTCGAGGGGTTGCG‑CACC‑GTCGGCCCGCGGGGAGGGCTAGAGGTGGACCCTTTTTCTGATAACCGGCCGAAAAGGGACCTCCCAGCGGGAGAATTTACGCCACGGGGCGCTTAAAAGAata                                                                                                            >  2:510409/1‑238 (MQ=255)                                                                                                                                       tCTTGTACTTCGCGAGAAAAACGGCG‑ACGGGTGCCAAAATCCA‑TCAGATGGAAGCGCGACATATCAAGACCGGC‑GGTTAAC‑GCCGAGAAG‑TCGAC‑TAATTTTCTTTTCAGCGTGTCGAGGGCTTGCG‑CAAC‑GTCGGCCTGCGGTGAGCGATAGCGGTGTACCATTTCACCTATAACCCCCAGCAAAGGAACTTTCCCGAGGGTGACCTCACGCCACGGGCCGCTTTAACCAAAATccc                                                                                                        >  2:455202/1‑237 (MQ=255)                                                                                                                                       tCTTGTACTTCGCGAGAAAAACGGCG‑ACGGGTGCCAAAATCCA‑TCAGATGGAAGCGCGACATATCAAGACCGGC‑GGTTAAC‑GCCCAGAAG‑TCGAC‑TAATTTGCTTTCCAGCCGGTCGAGGGCTTGCG‑CAAC‑CTCGGCCCGCGGTGGGCGCAAACGATGTACCCTTTTCATGTTAACCCCCAGCCAAAGAACTTCCCCCAGGATTACTTCCCCCCACGGGCCCCTTAAACgccaacccc                                                                                                        >  2:625725/1‑230 (MQ=255)                                                                                                                                        cTTGTACTGCGCGAAAAAAACGGCG‑AGGGGTGCAAAAATCCA‑TCAGAGGGAAGCGCGACATATCAAGACCGGC‑GGTTAAC‑GCCGAGAAG‑TCGAC‑TAATTTGCTTCCCAGCGTGTCGAGGGCTTGCG‑CAAC‑GTGGGCCTGCGGTGAGCGATAGCGATGTACCATTTCACTGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGc                                                                                                      <  1:1103907/239‑1 (MQ=255)                                                                                                                                               tgcgagagaAAAACGGCG‑A‑GGGGGCACAAACCCAGACAGGTGAACGCGGGAAATATCAAGCCGGGG‑GGTAACC‑GCCGAGAGG‑CCGTC‑TAATTTTCTTTCCAGGGTGTCGAGGGTTTGCG‑AACC‑GTCGGCCGGGGGTGGGCGATGGCGATGTACCTTTTCCCTGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCAt                                                                                               <  2:733590/234‑1 (MQ=255)                                                                                                                                               tccgggaaaaaaaCGGCG‑CCGGGTGCCAAAATCTA‑TCAGATGGAAGCGCGACATATCAAGACGGGC‑GGTAAGC‑GCCGAAATG‑TCGAC‑AAATTCTCTTGCCAGCGTGTCGAGGGCTTCCG‑CAAC‑GGCGGCCGGCGGTGAGCGATAGCGATGTCCCATTCCACTGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCAt                                                                                               <  2:47946/231‑1 (MQ=255)                                                                                                                                               tccgcgAAAAAACCGGCG‑CGGGGGGCCAAAGCCCT‑ACCGAGGGAACCGCGAAATAACAAGCCCGGC‑GGGTACC‑CCCTAGATT‑CCGAC‑AAATTTCTTTTCCAGCGTGGCGAGGGTTGGCC‑CACC‑GTGCGCCTGCGGTGGGTGATAGCGATGTCCTTTTCCACGGAAAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCAt                                                                                               <  2:252479/237‑1 (MQ=255)                                                                                                                                                 cgcgAGAAAAACGGCG‑ACGGGTGCCAAAATCCA‑TCAGATGGAAGCGCGACATATCAAGACCGGC‑GGTTAAC‑GCCGAGAAG‑TCGAC‑TAATTTGCTTTCCAGCGTGTCGGGGGGTTGCG‑CAAC‑GTCGGCCCGCGGGGAGCGCGAGCGATGGACCATTTTCCTGATAACCCCCCGCAAAGGGACTTTCCCGAGGGTGACTTCACGCCCCCGGCCGCTTAAAACAATAACCacccttccctct                                                                                             >  2:1294113/1‑228 (MQ=255)                                                                                                                                                 cgcgAGAAAAACGGCG‑ACGGGTGCCAAAATCCA‑TCAGATGGAAGCGCGACATATCAAGACCGGC‑GGTTAAC‑GCCGAGAAG‑GCGAC‑TAATTTGCTTTCCCGCGTGTCGGGGGCTTGCG‑CAAC‑GTCGGCCTGCGGGGAGCGATAGCGATGTACCATTTTACTGATAAACGCCAGCAAAGGAACTTCCCCGAGGATGACTTCACGCCCCGGGCCGCTTAAACGAATATCCAACTTGCCCTTa                                                                                             >  1:1160199/1‑239 (MQ=255)                                                                                                                                                 cgcgAGAAAAACGGCG‑ACGGGTGCCAAAATCCA‑TCAGATGGAAGCGCGACATATCAAGACCGGC‑GGTTAAA‑GCCGAGAAG‑TCGAC‑TAATTTGGTTTTCAGCGTGTCCGGGGCGTGCG‑CCAC‑GTCGGGCTGCGGTTAGCGAGAGCGATGTACCATTTTAATGATAACACCCAGCCAAGGAACTTCCCAGAGGATGGCGTTACGCCACGGGCCGCTTAAACGAAAAACCAACCTGCCagtt                                                                                             >  2:291933/1‑236 (MQ=255)                                                                                                                                                 cgcgAGAAAAACGGCG‑ACGGGTGCCAAAATCCA‑TCAGATGGAAGCGCGACATATCAAGACCGGC‑GGCTAAC‑GCCGCGAAA‑TCGGC‑TAAATTTCTTTCCCACGTGTCGAGGGTTTGCG‑CAAC‑GCCCGCCTTCGGTGAGCGACAGCGCTGGACCATTTGCCTGATAACCGCCAGCATCAGAACCTCCCAGAGGGTGACTTCCTCCCCCAGGCCGCCTTCACGAATACTGACCCTGCCATTa                                                                                             >  2:1232069/1‑239 (MQ=255)                                                                                                                                                      aaaaacCGGCG‑ACGGGTGCCAAAATCCA‑TAAGATGGAGGCGCGAATTACAAAGGCGGGC‑GGTTAAC‑GCCGAAAAG‑TCGAC‑TAATTTGTTTTCCAGCGTGCGGAGGGCGTGCG‑AAAC‑GCCGGTGGGCGGTGAGCAATAGCGATGTCCCATTTCACTGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGt                                                                                         <  2:641121/237‑1 (MQ=255)                                                                                                                                                       aaaacCGGCG‑GCGGGGGCAAAAACCCA‑GCAAGTGGAGGCGCGAAAAATCAAGCCGGGC‑GTTTACC‑GCCGAAAGG‑TCGAC‑TAATTTGCTTTCCAGGGTGTGGAGGCTTTGCG‑AAAC‑GTCGGCCGGGGGGGAGCGATGGCGTGGTACCATTTCACTGAAAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTg                                                                                       <  2:71015/239‑1 (MQ=255)                                                                                                                                                        aaaaCGGCG‑ACGGGTGCCAAAACCCA‑TCAGAGGGAAGCGCGACATATCAAGACCGGC‑GGTTAAC‑GCCGAGAAG‑TCGAC‑TAATTTGCTTTCCAGCGTGTCGAGGGCTTGCG‑CAAC‑GTCGGCCTGCGGTGAGCGATAGCGATGTACCATTTCACTGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTgg                                                                                      <  1:455202/239‑1 (MQ=255)                                                                                                                                                             ggcg‑gcGGGTGCCAAAATCCA‑TCAGAAGGAAGCGCGACATAACAAGACCGGG‑GGTTAAC‑GCGGAGAAG‑CCGAC‑TAATTTGCTTCCCAGCGGGGCGAGGGCTGGCG‑CACC‑GTCGGCCGGCGGTGAGCGATAGCGATGTACCATTCCACGAATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACg                                                                                 <  2:1190240/239‑1 (MQ=255)                                                                                                                                                              gcg‑gcgGTTGCAAAAACCAC‑TAAGAGGGAGCCGCGACATAACAAGCCGGGC‑GGTTAAC‑GCCGAGAAG‑TCGAC‑TAATTTTCTTTCCAGCGTGCCGAGGTTTTGCG‑CACC‑GTGGGCCTGCGGTGAGCGTGAGCGATGTCCTATTTCATTGAAAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATAGCCAGCTTGCCATTATCGTTGGACACg                                                                                 <  2:1315994/238‑1 (MQ=255)                                                                                                                                                                 gagggggGCAAAAACCCA‑TCAGAGGGAAGGGAAACATACCAGGCCCGGG‑TGTTACC‑GCCGAAAAG‑CCGAT‑TAATTTGCTTTCCGGGGTGTCGAGGGTTTGCG‑AACG‑GTGGGCCTGCGGTGGGAGTTAGCGATGTACCATTTCACTGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGt                                                                               <  2:731928/235‑1 (MQ=255)                                                                                                                                                                         ccAAAATCCA‑TCAGATGGAAGCGCGACATATCAAGACCGGC‑GGTTAAC‑GCCGAGAAG‑TCGAC‑TAATTTGCTTTCCAGCGCGTTGAGGGGTTGCG‑CCAC‑GTCGGCCCGCGGTGAGCGATAACGATGTACCATTTTACTGATAACAGCCAGCAAAGGCACTTCCCACAGGATGACTTCACCCCCCGGGCCGCTTAAACCAATATCCAGCCTGCCCTTTTCGTTGGACAACGGGAC‑Ttttt                                                                      >  2:767963/1‑238 (MQ=255)                                                                                                                                                                          aaaaaCCCA‑TGAGATGGAAGCGGGACATATCAAGACCGGC‑GTTTACC‑GCCGAGAAG‑TCGAC‑TAATTTGCTTCCCAGCGTGTCGAGGGCTTGCG‑AAAC‑GTCGGCCTGGGGTGAGCGATAGCGATGTACCTTTTCACTGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGAC‑TTGTTc                                                                     <  2:386035/237‑1 (MQ=255)                                                                                                                                                                               tCCA‑TCAGATGGAAGCGCGACAAAGCACGCCCGGT‑GTTTACC‑GCCGAGAAG‑TCGAC‑TATTTTGCTTTCCGGCGTGCCGAGGGCTTGCG‑CACC‑GTCGGCCTGCGGTGAGCTATAGCGATGTCCCATTCCACTGAAAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGAC‑TTGTTCCGGGtt                                                               <  2:150269/239‑1 (MQ=255)                                                                                                                                                                                ccA‑TCAGATGGAAGCGCAACTTATCAGGCCCGGC‑GGTTACC‑GCCGAGAAG‑TCGCT‑TATTTTGCTTCCCGGGGTGTGGAGGGCTGGCG‑CAAC‑GCCGGCCGGCGGGGAGCGATGGCGAGGTACCATTTCACTGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGAC‑TTGTTCCGGGTTa                                                              <  2:1160199/239‑1 (MQ=255)                                                                                                                                                                                 cA‑TCAGATGGAAGCGCGACATATCAAGACCGGC‑GGTTAAC‑GCCCAGAAG‑TCGAA‑TAATTTGCTTTTCAGCGTGGCGAGGGGTTGCG‑CCAC‑GTCGGCCCGCGGGGAGGGGTAGCGATGTACCATTTCCATGATAACCCCCCGCACAGGGAATTTCCCGAGGATGGCTTTACGCCACGGGCCCCCTAAACGAAAATCCAGCTTGCCCTTCTCGTTGGACACGGCGCC‑TTTTTCCGGGGTac                                                             >  2:1287093/1‑238 (MQ=255)                                                                                                                                                                                          ggAAGCGCGACATATCAAGACCGGC‑GGTTAAC‑GCCGAGAAG‑TCGAC‑TAATTTGCTTTCCAGCGTGTCGAGGGGTTGCG‑CAAC‑GGCGGCCTGCGGTGAGCGGTAGCGATGTACCATTTCACTGATAACCGCCAGCAAAGGAACTTCCCAGAGGGTGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCCTGCCATTATCGTTGGACACGGTGAC‑TTGTTCCGGGTTAAAACGGa                                                       >  1:1022130/1‑237 (MQ=255)                                                                                                                                                                                           aacgcggcaCATAGCAAGCCCGGC‑GTTAACC‑GCCGAGAAG‑GCGAC‑TAATTTGCTTCCCGGGGGGTCGGGGGCTTGCG‑CAAC‑GTCGGCCGGGGGTGAGCGATAGCGATGTACCATTCCCCTGATACCCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGAC‑TTGTTCCGGGTTAAAGCGGaa                                                      <  2:884048/229‑1 (MQ=255)                                                                                                                                                                                            aaGCGCGACATATCAAGACCGGC‑GGTTAAC‑GCCGAGAAA‑TCGAC‑TAATTTGCTTTTCCGCGGGTCGAGGGCCTGCG‑CAAC‑GGCGGCCTGCGGGGGGCGGGAACGATGTACCCTTTTACCGATAACCGCCCGCCAAGGAAATTCCCCCAGGATGACTTCACGCCACGGGCCGCGTAAACGAAAATCCCCATTGCCCTTATCTGTGGACACAGGGGC‑ATTGTCCGGGGTTAAGCGGGAcac                                                   >  2:289260/1‑237 (MQ=255)                                                                                                                                                                                                  gACATATCAAGACCGGC‑GGTTAAC‑GCCGAGAAG‑TCGAC‑TAATTTGCTTTCCGGCGTGTCGAGGGCTTGCG‑CAAC‑GTCGGCCTGCGTTGAGCGATAGCGATGTACCATTTCACGGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGAC‑TTGTTCCGGGTTAAAGCGGAACTCGCGTaa                                             <  1:1175243/239‑1 (MQ=255)                                                                                                                                                                                                   caatatCAGGCCCGGG‑GGTTAAC‑GCCGAGAAG‑TCGAC‑AAATTTGTTTCCCAGCGTGTGGGGGGTTGGCG‑CAAC‑GTCGCCCGGGGGTGAGCGAGAGCGATGTACCATTTCACTGAAACCCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGAC‑TTGTTCCGGGTTAAAGCGGAACTCGCGTaa                                             <  2:139925/236‑1 (MQ=255)                                                                                                                                                                                                   acaaaacAAGACCGGG‑GGTTAAC‑GCCGAGAAG‑TAGCT‑TAATTTGTTTCCCAGCGTGGCGAGGGCTTGCG‑AAAC‑GCGGGCTGGGGGTGGGCGATAGCGATGTCCCATTCCACTGATACCCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGAC‑TTGTTCCGGGTTAAAGCGGAACTCGCGTaa                                             <  2:314892/232‑1 (MQ=255)                                                                                                                                                                                                       atCAAGACCAGC‑GCTTAAG‑CCCAAAAAC‑TCGTG‑CAATTTGTTCTCCTGCGTGACGAGGGCTGGCA‑CATC‑GACAGCTTGCGGTAAGTCATAAGGTTGTACTAATCCCCGAAAAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCAAGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGAC‑TTGTTCCGCGTTAAAGCAGAACTCGCGTAACCAGt                                        <  2:1220753/239‑1 (MQ=255)                                                                                                                                                                                                         aaaGCCCGGC‑GGTTAAC‑GCGGAGAAG‑TGGAC‑TAATTTGCTTTGCAGCGTGTGGAGGGTTTGCG‑CAAC‑GTGGCCCTGCGGTGAGGAATAGCGTTGTACAATTTCATGGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGAC‑TTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTa                                      <  1:980556/238‑1 (MQ=255)                                                                                                                                                                                                               cggc‑ggTTTAC‑GCCGAGAAG‑TCGAC‑TAATTTTCTTTTCAGCGTGTCGAGGGCTTGCG‑CAAC‑GGCGGCCTGCGGGGAGCGATAGCGATGTACCATTTTACTGATAACCGCCCGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGGGAC‑TTGTTCCGGGTTAAAACGGGACTCGCGCAACCCGTTAAGATAg                                >  2:120841/1‑239 (MQ=255)                                                                                                                                                                                                                ggc‑gtTTACC‑GCCGAAAAG‑TCGAC‑TAATTTGCTTTCCGGCGTGTCGGGGGCTTGCG‑AAGC‑GGGGCCCTGCGGTGAGCGATAGCGATTTACCATTTCACTGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGAC‑TTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGt                               <  1:448960/239‑1 (MQ=255)                                                                                                                                                                                                                ggc‑ggTTAAC‑GCCGAGACG‑TCGAC‑TAATTTGCTTTCCAGCGTGTCGAGGGCTTGCG‑CAAC‑GTCGGCCGGGGGGGAGCGATAGCGATGTCCCATTTCACTGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGAC‑TTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGt                               <  1:1287093/239‑1 (MQ=255)                                                                                                                                                                                                                 gggggTTAAC‑GCGGAGAGG‑TCGAC‑AAATTTGCTTTCCAGCGTGTGGAGGGCTTGCT‑CAAC‑GTCGGCCGGCGGTGGGGTATGGCGATGTACATTTTCACGGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGAC‑TTGGTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGt                               <  1:414600/236‑1 (MQ=255)                                                                                                                                                                                                                 gc‑ggTTACC‑GCCGAGAAG‑TCGAC‑TAATTTGCTTTCCAGCGTGTCGAGGGTTTGCG‑CAAC‑GCCGGCCTGGGGTGAGCGATAGCGATGTACCATTTCACTGAAAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGAC‑TTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGt                               <  1:173858/238‑1 (MQ=255)                                                                                                                                                                                                                 gc‑ggTTAAC‑GCCGGGAAG‑TCGAC‑TAATTTTCTTTCCAGCGGGTCGAGGGCTTGCG‑CAAC‑CTCGGCCTGCGGTGAGCGATAGCGATGGACCATTTCACTGATAACCGCCAGCAAAGGAACTTCCCCGAGGGTGACTTCACGCCCCGGGCCGCTTAAACGAATATTCCGCTTGCCATTATCGTTTGACACCGGGAC‑TTGTTCCGGGGTAAAACGGGAATCGCGGCACCAGTTAAGATAATc                              >  2:1256634/1‑239 (MQ=255)                                                                                                                                                                                                                 gc‑ggTTAAA‑GCCGCGAAG‑TCGCC‑TAAATTTCTTTCCAGCGGGTCGAGGGGTTGCG‑CAAC‑GCCGGCCCGCGGTGAGCGATAGCGATGTACCATTTCAATGATAACCGCCAGCAAAGGAAATTCCCCCAGGACGCCTTCACGCCCCGGGCCGCTTAAACGAACATCCCACTTGCCATTATCCTTGGGCCCGGTGACTTTTTTCTGGGTAAAAGC‑GCACCCGCGTAACCCGTTTAGATa                                 >  2:1074776/1‑236 (MQ=255)                                                                                                                                                                                                                        tatggCCGAGAAG‑CCGAT‑TATTTGCCTTTCCAGCGGGTCG‑GGGGTTGGC‑CAACAGTCGGCTGGCGGGGAGGCAGAGCGATGTCCCATTTCACTTATAGCCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGAC‑TTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCt                         <  2:373708/235‑1 (MQ=255)                                                                                                                                                                                                                                agaAG‑TCGAC‑TAATTTGCTTTCCAGCGTGTCGAGGGCTTGCG‑CAAC‑GTCGGCCTGCGGTGAGCGATAGCGATGTACCATTTCACTGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGAC‑TTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCTTaaagaaa                 >  2:335453/1‑239 (MQ=255)                                                                                                                                                                                                                                      tCGAC‑TAATTTGCTTTCCAGCGTGTCGAGGGCTTGCG‑CAAC‑GTCGGCCTGCGGTGAGCGATAGCGATGTACCATTTCACTGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGAC‑TTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCTTAAAGAAAggcag            >  1:406275/1‑239 (MQ=255)                                                                                                                                                                                                                                              aTTTGCTTTCCAGCGTGTCGAGGGCTTGCG‑CAAC‑GTCGGCCTGCGGTGAGCGATAGCGATGTACCATTTCACTGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGAC‑TTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCTTAAAGAAAGGCAGGGCAGaa     >  1:305231/1‑239 (MQ=255)                                                                                                                                                                                                                                              aTTTGCTTTCCAGCGTGTCGAGGGCTTGCG‑CAAC‑GTCGGCCTGCGGTGAGCGATAGCGATGTACCATTTCACTGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGAC‑TTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCTTAAAGAAAGGCAGGGCAGaa     >  1:197312/1‑239 (MQ=255)                                                                                                                                                                                                                                                 tGCTTTCCAGCGTGTCGAGGGCTTGCG‑CACC‑GTCGGCCTGCGGTGAGCGATAGCGATGTACCATTTCACTGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGAC‑TTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCTTAAAGAAAGGCAGGGCAGAAAGc  <  1:1276399/239‑1 (MQ=255)                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                 GGCTGATTTGCTGATGTGCCTGGAACCATTCGTGTGCCTGTGTTCCCATCGGCGTGAGGGAAAGCCGACGCGCCAGATCGTAGTTGCTGGTGCCCACAAACCAGGATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCG‑ACGGGTGCCAAAATCCA‑TCAGATGGAAGCGCGACATATCAAGACCGGC‑GGTTAAC‑GCCGAGAAG‑TCGAC‑TAATTTGCTTTCCAGCGTGTCGAGGGCTTGCG‑CAAC‑GTCGGCCTGCGGTGAGCGATAGCGATGTACCATTTCACTGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGAC‑TTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCTTAAAGAAAGGCAGGGCAGAAAGC  >  NC_000913/989666‑990138

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑