Predicted mutation | |||||||
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evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | NC_000913 | 2,151,681 | G→T | 19.0% | intergenic (+35/+30) | yegJ → / ← yegK | uncharacterized protein/ser/thr phosphatase‑related protein |
Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 2,151,681 | 0 | G | T | 19.0% | 32.1 / 2.5 | 35 | intergenic (+35/+30) | yegJ/yegK | uncharacterized protein/ser/thr phosphatase‑related protein |
Reads supporting (aligned to +/- strand): ref base G (14/9); new base T (3/3); total (17/18) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 6.69e-01 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.76e-01 |
ATGTTTATTGGCGTAATCAGCAATGACCCTGGTATTGTTACAAACGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAG‑ATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTATACCATTGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGG‑TTG‑AA‑TTGTTGTCTT‑CAA‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCAT‑CCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGCATATTTAACGCCAGTCGCTGGATA > NC_000913/2151443‑2151912 | aTGTTTATTGGCGTAATCAGCAATGACCCTGGTATTGTTACAAACGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAG‑ATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTATACCATTGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGG‑TTg < 1:561242/239‑1 (MQ=255) taatGGCGTAATCAGCAATGAACCTGGTATTGTTAAAAACGTAGAATATGGCTAGTATTGGAAAATCAAGAAAGAAG‑ATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTATACGATTGATCCTTTATTGGTCGCCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGG‑TTG‑AA‑ttg < 2:947592/236‑1 (MQ=255) tATTGGCGTAATCAGCAATGACCCTGGTATTGTTACAAACGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAG‑ATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTATACCATTGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGG‑TTG‑AA‑ttg < 1:396693/239‑1 (MQ=255) tAATCAGCAATGACCCTGGTATTGTTACAAACGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAG‑ATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTATACCATTTATTCTTTTTTTGTTACCTTTCCAAAAGAAGAAAACGACGAGCGAAGAGCAAAAATAGTTTCCTTATCAAACGTTATATTTTTTTTTTAAAATAGCGG‑TGT‑AA‑TTTTTGttttt > 1:699365/1‑238 (MQ=255) gcAGGGGGGGAAAAAAAAAAAAAAAGAATATTTAAGGTTGGATGTATACGCGGGGGGAAAAAATTTCGGGGGTTTATACCATTGATCTTTTTTTGGTCCCCTATCAAAAAGAAAACGCCGAAGAGAAAAGAGAAAAATTAGTGCGTTAATCAGCCTTTTTTTTTTTTTTTAAAACGGGGGG‑TTG‑AA‑TTGTTGTCTT‑CAA‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGcc < 2:468798/237‑1 (MQ=255) aGTAGAAAAACAAAAAAGAAT‑ATAGTTAAGACTGGTGTTATAGGCGGGGGAATAAATTTCGCGGGTGTTATACATTTAATCCTTTATGGCCCTCCAACCAAAAAGAAAAAGCGGATGAGCTAAGAAAAAAATTAGTTCGATAGCCAGCGTTTATATTTTTTGTTTAACCGGGGGG‑TTG‑AA‑TTGTTTTCTC‑AAC‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGtt < 2:972473/238‑1 (MQ=255) aaTAAAAAAAAAAG‑ATATTTCAGCCTGGATGTATCCGCGGGGGAAAAAAATTTACGGCGGTTATCCCATTGTTCTTTTATTGCTCACCTACCAAAAAGAAGAAGCCAACGAGAAAAGAAAAAAATATGTTCGTTAATCACCCTTTATATTTATTGTTTAACCTGGGGG‑TTA‑AA‑TTTTTGTCTT‑CAA‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTatcatc < 1:443884/239‑1 (MQ=255) tacctcatgcTGGAGTTACACGCGCTGGAATAAAATTTACGGGCTTTACACTTTGGACCCTTTATGGCACACCTAGCAAAAAAAAGGAGGCGACAGGTCAAGAAAAAAAAGTCTGCGCTACACACCCGTTTTATTTATTGTTTAGCCTGGGGG‑TAA‑AT‑TTGTT‑TCCT‑CAACCCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCAT‑CCGTACGCTCGtt < 2:115863/230‑1 (MQ=255) gTTATACCATTGATCCTTTATTGGTCCCCTACCAAAAAGAAAAAGCCGAGGAGCTAAGAGAAAAATTAGTCCGCTAACCAGCCGTTATATTTATTGTTTAAACGGGCGG‑TTG‑AA‑TTGTTGTCTT‑AAA‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTatcatcat < 1:211309/182‑1 (MQ=255) ctttGATCTTTTATTGGCCCCCTACCAAAAAGAAAAAGCCGAAGAGCTAAGAGAAAAATTAGTTCGTTAATCAGCTTTTATTTTTTTTTTTAAAACGGGGGT‑TTA‑AT‑TTTTTTTCTC‑AAA‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCAT‑CCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAg < 1:544188/237‑1 (MQ=255) tGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGG‑TTG‑TA‑TTTTTTTTTT‑CCA‑CCGCCATGAGAAAATCACTCTTCCCCCAGCGCCAGTGCCAACGTTTTATCCTCCT‑CCGTACCCTc > 2:211309/1‑182 (MQ=255) gATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGG‑TTG‑AA‑TTGTTGTCTT‑CCA‑CCGCCATGAGGAAATCAATCTGCCCCCAGCGCCAGTGCCAACCGTTTATCATTAT‑CCGTTAGCCCGTGTAACGCCGGGCTGGACCAAAACTGCTTTTACAATTTCGGTTATAAATCGAgttgt > 1:430787/1‑239 (MQ=255) ttATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGG‑TTG‑AA‑TTGTTGTCTT‑CAA‑CCGCCATGAGAAAATCACTCTGCCCACAGCGCCAGTGCCAACGGTTTTTCATCAT‑CCGTAAGCTCGTTTACCGCCGGGCTGGACAAAAAATGCTTTAACAATTCAGGTAATAAAACGAGTTGTTCCTGc > 1:567766/1‑239 (MQ=255) tATTGCTCCCCTACCAAAAAAAAAAAGCCGACGAGCTAAGAGCAAAATTATTCCGTAAATCAGCTGTTATATTTATTTTTAAAACTGGGGG‑TTA‑AA‑TTGTTGTCTT‑CAC‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCAT‑CCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCg < 1:914921/239‑1 (MQ=255) gTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGG‑TTG‑AA‑TTTTTGTCTT‑CAA‑CCCCCATGAGAAACTCACTCTGCCCCCAACGCCCGTGCCCACGGTTTTTCATCAT‑CCGTACGCTCGTTTACCGCCCGGCTGGACAAAAACATCTTTAACAATTTAGGGAATAAATCGAGTTGTTCCTGCGTtgct > 2:268980/1‑239 (MQ=255) tCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGG‑TTG‑TA‑TTGTTGTCTT‑CAA‑CCGCCATGAGAAACTCACTCTGCCCCCAGCGCCAGTGCCCACGGTTTATCATCAT‑CCGTACACTCGTTTTCCGCCCGGCCGGACAAAAACTGCTTTAACCAATCCGGGAATAAATCGAGTTGTTTCttggtttct > 2:977994/1‑230 (MQ=255) tCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGG‑TTG‑AA‑TTGTTGTTTT‑CAA‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCAT‑CCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGGTGTTTCTGCGTtgct > 1:897092/1‑238 (MQ=255) aCCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGG‑TTG‑AA‑TTGTTGTCTT‑CAA‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCAT‑CCGTACGCCCGTTTACCCCCCGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAAACGAGTTTTTTCTGCggttcttc > 1:1091478/1‑231 (MQ=255) aaGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGG‑TTG‑AA‑TTGTTGTGTT‑CAA‑CCCCCATTAGAAAATCACTCTGCCCCCAGCGCCAGTGCCAACGGTTTATCATCAT‑CCGTACGCTCGTTTACCGCCGGGGTGGGCAAAAACTGCTTTCACAATTTCGGCAATAAATCGAGTTTTTCCTGCGGTTCCGCCGCCAGTCCCATGGAAAACGGGGGaaagca > 2:749916/1‑237 (MQ=255) aaaaTTTGTTCCATAAACAGCCCTTATTATTTTTTTTTAAACCGGGGG‑TTT‑AATTTTTTGTTTT‑CAA‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCAT‑CCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGca < 1:295498/239‑1 (MQ=255) aaaaTTGGTTCCTTAATAACCCTTTATTTTTTTTGTTAAACCTGG‑GG‑TTT‑AA‑ATTTTTTCCT‑CAACCCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCAT‑CCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGcac < 1:500990/239‑1 (MQ=255) tAGTTCGTTATACACCCTTTTTTTTTTTTATTAAACCTGG‑GG‑TTT‑AA‑TTTTTTTTTTCCAA‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCAT‑CCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATgag < 1:99038/239‑1 (MQ=255) tgtccatAATCAGCCGTTATTTTTATTGTTAAAACTGGGGG‑TTG‑AA‑TTTTTGTCTT‑AAA‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCAT‑CCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATgagga < 1:307402/233‑1 (MQ=255) cTAATCAGCCGTTATATTTATTGTTTAAACTGGCGG‑ATG‑TA‑TTGTTTTTTT‑TCA‑ACGCCATGAGAAAATAACTCTGCCCACAACCCCCATGCCCACGTTTTTTTATCATCCCGTAC‑CTCTGTTACCGCCGGGGTGGACAAAAAATTCTTTTACAATTCATGGAATAAATAGCGGTGTTTCTGCGGTTCTTCCGCCAGTCCATTGTAAAACGGGGAAAAAAAAGGCACATGAGGGGAAtt > 2:1231099/1‑239 (MQ=255) acaaGCCTTTTTATTTATTGTTAAACCGGGCGG‑TTG‑AT‑TTTTTTTCTT‑CAA‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCAT‑CCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATc < 1:872061/236‑1 (MQ=255) ccGTTATATTTATTGTTTAAACTGGCGG‑TTG‑GA‑TTGATGTCTT‑CCG‑ACGCCATCAGAAAATCACTCTTCCCCCAACGCCAGGGCCAACCTTTTATCATCAT‑CCGAAAGCTCGTTTTTCCCCGGGCTGCACCAAAAATGCTTTAACAATTCCAGCAATAATTCATGTTGTTCCGGCGTTGCTGCCGCCAGTCCATTGAAAAAAGGGATAAACAAATGCACATGAGGAGAAGTATCCAGCa > 2:636701/1‑239 (MQ=255) attGTTTAAACTGGCGG‑TTG‑AA‑TTGTTTTCTT‑CAA‑CCGCCTTGAGAAAATCACTCTGACCACAGCGCCAGTGCCAACGTGTTTTCATCAT‑CCGTTCGCTCGTTTAACGCCGCGCTGGACAAAAAATTCTTTTACAATTCAGGTAATAAATTGAGTTGTTTCTGCGTTGCTGCCGCCAATCCATTGGAAAAAGGGGAGAAAAAAGGGACAATAGGAGAATTATCCAGGATATTTAACGcc > 1:1331392/1‑239 (MQ=255) attattAAACCGGCGGTTTG‑AC‑TGTTTGTCAC‑CAA‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCAT‑CCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGCATATTTAACGcc < 1:1231099/235‑1 (MQ=255) tGTTTAAACTGGCGG‑TTT‑AA‑TTGTTGTCTT‑CAA‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGGTTTATCATCAT‑CCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAAAGGGGGAAAGAAAGGCACATGAGGAGAATTATCCAGCATATTTAACGCCAg > 1:753409/1‑239 (MQ=255) tGTTTAAACTGGCGG‑TTT‑AA‑TTGTTGTCTT‑CAA‑ACCCCATGAGAAACTCACTCTGCCCACAGCGCCCGTGCCAACGTTTTATCATCAT‑CCGTACGCTCGTTTACCGCCGGGCGGGACAAAAACTGCTTTTACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTTCTGCCGCCCGTCCCTTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGCATATTTAACGCCAg > 1:760198/1‑239 (MQ=255) tGTTTAAACTGGCGG‑TTG‑AA‑TTGTTGTTTT‑CAA‑CCGCCATGAGAAAATCACTCTGCCCCCAACGCCAGTGCCCACGTTTTATCATCAT‑CCGTACGCCCGTTTACCGCCGGGCTGGACAAAAAATGCTTTTACAATTCAGGTAATAAACTGAGTTGTTCCTGCGGTGCTGCCGCCCGTCCATTTGAAAACGGGGGAAAGAAAAGCACAAGAGGAGGATTATCCAGCCTATTTAACGcccg > 1:66511/1‑237 (MQ=255) tGTTTAAACTGGCGG‑CTG‑AA‑TTGTTGTCTT‑CAA‑CCGCCATGAGAAACTCACCCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCAT‑CCGTACGCCCGTTTTCCGCCGGGCTGGACAAAAACTTCTTTAACAATTCCGGTAATAAATCGAGTTGTTCCTGCGGTTCTGCCGCCAGTCCATTGAAAAACGGGGGAAAGAAAGGCACATGAGGAGAATTATCCAGCATATTTTACGCCaa > 1:884981/1‑238 (MQ=255) tGTTAAAACGGCCGT‑TTA‑AT‑TTTTTGCCTA‑CAC‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCAT‑CCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGCATATTTAACGCCAg < 1:1095048/239‑1 (MQ=255) tttAACCGGGGGG‑TTA‑AA‑TTGTTGTCTT‑AAA‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCAT‑CCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAg < 1:1315240/150‑1 (MQ=255) ggg‑TTGAAA‑TTGTTTTTTC‑CAA‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCAT‑CCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGCATATTTAACGCCAGTCGCTGGATa < 1:1012115/237‑1 (MQ=255) cGG‑TTG‑AA‑TTTTTTTCTT‑TAA‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGGTTTATTATCAT‑CCGTACGCCCGTTTTCCCCCGGGCTGGACAAAAACTGCTTTTACAATTCAGGGAATAAATCGAGTTGTTTCTGc > 2:1315240/1‑150 (MQ=255) | ATGTTTATTGGCGTAATCAGCAATGACCCTGGTATTGTTACAAACGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAG‑ATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTATACCATTGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGG‑TTG‑AA‑TTGTTGTCTT‑CAA‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCAT‑CCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGCATATTTAACGCCAGTCGCTGGATA > NC_000913/2151443‑2151912 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |