| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 2,807,369 | A→C | 15.6% | T80P (ACC→CCC) | proX → | glycine betaine/proline ABC transporter periplasmic binding protein; cold shock protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,807,369 | 0 | A | C | 15.6% | 217.5 / 4.9 | 74 | T80P (ACC→CCC) | proX | glycine betaine/proline ABC transporter periplasmic binding protein; cold shock protein |
| Reads supporting (aligned to +/- strand): ref base A (54/7); new base C (2/11); total (56/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.78e-07 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GCGACATAGCGTACTTTTTGCGACAGCGTTTGCCACGCTTATCTCTACACAAACTTTTGCTGCCGATCTGCCGGGCAAAGGCATTACTGTTAATCCAGTTCAGAGCACCATCACTGAAGAAACCTTCCAGACGCTGCTGGTCAGTCGTGCGCTGGAGAAATTAGGTTATACCGTCAACAAACCCAGCGAAGTAGA‑TTACAACGTTGGCTA‑CACCTCGCTTGCTTCCGGCG‑ATGCAACCTTCACCGCCGTGAACTGGACGCCACTGCATGACAACATGTACGAAGCTGCCGGTGGCGATAAGAAATTTTATCGTGAAGGGGTATTTGTTAACGGCGCGGCACAGGGTTACCTGATCGATAAGAAAACCGCCGACCAGTACAAAATCACCAACATCGCACAACTGAAAGATCCGAAGATCGCCAAACTGTTCGATACCAACGGCGACGGAAAAGCGGATTTAACCGGTTGTA > NC_000913/2807134‑2807603 | gCGACATAGCGTACTTTTTGCGACAGCGTTTGCCACGCTTATCTCTACACAAACTTTTGCTGCCGATCTGCCGGGCAAAGGCATTACTGTTAATCCAGTTCAGAGCACCATCACTGAAGAAACCTTCCAGACGCTGCTGGTCAGTCGTGCGCTGGAGAAATTAGGTTATACCGTCAACAAACCCAGCGAAGTAGA‑TTACAACGTTGGCTA‑CACCTCGCTTGCTTCCGGCG‑ATGCAACCt > 1:628787/1‑239 (MQ=255) cGACATAGCGTACTTTTTGCGACAGCGTTTGCCACGCTTATCTCTACACAAACTTTTGCTGCCGATCTGCCGGGCAAAGGCATTACTGTTAATCCAGTTCAGAGCACCATCACTGAAGAAACCTTCCAGACGCTGCTGGTCAGTCGTGCGCTGGAGAAATTAGGTTATACCGTCAACAAACCCAGCGAAGTAGA‑TTACAACGTTGGCTA‑CACCTCGCTTGCTTCCGGCG‑ATGCAACCt > 2:122829/1‑238 (MQ=255) aGCGTACTTTTTGCGACAGCGTTTGCCACGCTTATCTCTACACAAACTTTTGCTGCCGATCTGCCGGGCAAAGGCATTACTGTTAATCCAGTTCAGAGCACCATCACTGAAGAAACCTTCCAGACGCTGCTGGTCAGTCGTGCGCTGGAGAAATTAGGTTATACCGTCAACAAACCCAGCGAAGTAGA‑TTACAACGTTGGCTA‑CACCTCGCTTGCTTCCGGCG‑ATGCAACCTTCAccg > 2:607118/1‑238 (MQ=255) cGTACTTTTTGCGACAGCGTTTGCCACGCTTATCTCTACACAAACTTTTGCTGCCGATCTGCCGGGCAAAGGCATTACTGTTAATCCAGTTCAGAGCACCATCACTGAAGAAACCTTCCAGACGCTGCTGGTCAGTCGTGCGCTGGAGAAATTAGGTTATACCGTCAACAAACCCAGCGAAGTAGA‑TTACAACGTTGGCAA‑CACCTCGCTCGCTTCCGGCG‑ATGCAACCTTCAcc > 1:907067/1‑235 (MQ=255) tACTTTTTGCGACAGCGTTTGCCACGCTTATCTCTACACAAACTTTTGCTGCCGATCTGCCGGGCAAAGGCATTACTGTTAATCCAGTTCAGAGCACCATCACTGAAGAACCCTTCCAGACGCTGCTGGTCAGTCGTGCGCTGGAGAAATTAGGTTATACCGTCAACAACCCCAGCGAAGTAGA‑TTACAACGTTGGCTA‑CACCTCGCTTGCTTCCGGCG‑ATGCAACCTTCACCGCCGTg < 1:22746/239‑1 (MQ=255) tACTTTTTGCGACAGCGTTTGCCACGCTTATCTCTACACAAACTTTTGCTGCCGATCTGCCGGGCAAAGGCATTACTGTTAATCCAGTTCAGAGCACCATCACTGAAGAAACCTTCCAGACGCTGCTGGTCAGTCGTGCGCTGGAGAAATTAGGTTATACCGTCAACAAACCCAGCGAAGTAGA‑TTACAACGTTGGCTA‑CACCTCGCTTGCTTCCGGCG‑ATGCAACCTTCACCGCCGTg > 2:1075542/1‑239 (MQ=255) tttttGCGACAGCGTTTGCCACGCTTATCTCTACACAAACTTTTGCTGCCGATCTGCCGGGCAAAGGCATTACTGTTAATCCAGTTCAGAGCACCATCACTGAAGAAACCTTCCAGACGCTGCTGGTCAGTCGTGCGCTGGAGAAATTAGGTTATACCGTCAACAAACCCAGCGAAGTAGA‑TTACAACGTTGGCTA‑CACCTCGCTTGCTTCCGGCG‑ATGCACCCTTCACCGCCGTGa > 1:338785/1‑237 (MQ=255) tttttGCGACAGCGTTTGCCACGCTTATCTCTACACAAACTTTTGCTGCCGATCTGCCGGGCAAAGGCATTACTGTTAATCCAGTTCAGAGCACCATCACTGAAGAAACCTTCCAGACGCTGCTGGTCAGTCGTGCGCTGGAGAAATTAGGTTATACCGTCAACAAACCCAGCGAAGTAGA‑TTACAACGTTGGCTA‑CACCTCGCTTGCTTCCGGCG‑ATGCAACCTTCACCGCCGTGa > 2:882597/1‑237 (MQ=255) tttGCGACAGCGTTTGCCACGCTTATCTCTACACAAACTTTTGCTGCCGATCTGCCGGGCAAAGGCATTACTGTTAATCCAGTTCAGAGCACCATCACTGAAGAAACCTTCCAGACGCTGCTGGTCAGTCGTGCGCTGGAGAAATTAGGTTATACCGTCAACAAACCCAGCGAAGTAGA‑TTACAACGTTGGCTA‑CACCTCGCTTGCTTCCGGCG‑ATGCAACCTTCACCGCCGTGAACTg > 1:347391/1‑239 (MQ=255) tGCGACAGCTTTTGCCACGCTTATCTCTACACAAACTTTTGCGGCCGATCTGCCGGGAAAAGGCATTACTGTTAATCCAGTTCAGAGCACCATCACTGAAGAAACCTTCCAGACGCTGCTGGTCAGTCGTGCGCTGGAGAAATTAGGTTATACCGTCAACAACCCCAGCGAAGTAGA‑TTACAACTTTGGCTC‑CCCCTCGCTTGCTTCCGGCG‑ATGAACCCTTCACCGCCGTGAACTGGa < 1:1276681/239‑1 (MQ=255) tGCGACAGCTTTTGCCACGCTTATCTCAACAAAAACTTTTGCGCCCGATCTGCGGGGCAAAGGCATTCCTGTTAATCAATTTCAGAGCCCCATCACTAAAGAACCCTCCCAGACCCTGCTGGTCAGTCGTGCGCTGGAAAAATTGGGTTATACCGTCAACAACCCCAGCGAAGTAGA‑TTACAACGTTGGCTA‑CCCCTCGCTTGCTTCCGGCG‑ATGCACCCTTCACCGCCGTGAACTGGa < 2:1016732/239‑1 (MQ=255) gCGACAGCGTTTGCCACGCTTATCTCTACACAAACTTTTGCTGCCGAGCTGCCGGGCAAAGGCATTACTGTTAATCCAGTTCAGACCACCATCACTGAAGAAACCTTCCAGACGCTGCTGGTCAGTCGTGCGCTGGCGATATTAGGCTATACCGTCACCAAACCCAGAGAAGTAGA‑TTACAACGATGGCTA‑CACCTCGCCTTCTTCCGGCG‑ATGCCCCCTTCACCGCCGTGAACTGGa > 2:1194570/1‑238 (MQ=255) gACAGCTTTTGCCACGCTTATCTCTACACAAACTTTTGCTGCCGATCTGCCGGGCAAAGGCATTACTGTTAATCCAGTTCAGAGCACCATCACTGAAGAACCCTTCCAGACGCGGCTGGTCAGGCGTGCGCTGGAGAAATTAGGTTATACCGTCAACAACCCCAGCGAAGTAGA‑TTACAACGTTGGCTA‑CACCTCGCTTGCTCCCGGCG‑ATGCAACCTTCACCGCCGTGAACTGGACGc < 1:1105976/239‑1 (MQ=255) cggCGTTTGCCACGCTTATCCCTACACAAATTTTTGCGGCCGATCTCCCGGGCAAAGGCATTACTGTTAACCCAGTCAAGAGCACCATCACAGAAGAACCCTTCCAGACGCTGCTGGTCAGTCGTGCGCTGAAGAAATTAGGTTATACCGTCAACAACCCCAGCGAAGTAGA‑TTCCAACGTTGGCTA‑CCCCTCGCTTGCTTCCGGCG‑ATGCACCCTTCACCGCCGTGAACTGGACGCCa < 2:869047/237‑1 (MQ=255) cACGCTTACCTCTACACAAACTTTTGCGGCCGATCTGCCGGGCAAAGGCATTACTGTTAATCCAGTTCAGAGCACCATCACTGAAGAAACCTTCCAGACGCTGCTGGTCAGTCGTGCGCTGGAGAAATTAGGTTATCCCGTAAACAACCCCAGCGAAGTAGT‑TTACAACGTTGGCTA‑CACCTCCCTTGCTTCCGGCG‑ATGAACCCTTCACCGCCGTGAACTGGACGCCACTGCATGac < 1:117228/238‑1 (MQ=255) aaaCTTTTGCTGCCGATCTGCCGGGCAAAGGCATTACTGTTAATCCAGTTCAGAGCACCATCACTGAAGAAACCTTCCAGACGCTGCTGGTCAGTCGTGCGCTGGAGAAATTAGGTTATACCGTCAACAAACCCAGCGAAGTAGA‑TTACAACGTTGGCTA‑CACCTCGCTTGCTTCCGGCG‑ATGCAACCTTCACCGCCGTGAACTGGACGCCACTGCATGACAACATGTACGAAGCTGcc > 2:1279474/1‑239 (MQ=255) aaCTTTCGCCGCCGAGGTGCCGGGCAAAGCCATGATTCAGAATCCAGCTAAGAACACCACCCAGAAAGAACCCTGCCAGACGCTGCGGGTCACTCGCACGATGGAGAAATTAGTTTATCCCGTCAAAAAACCCGGCAAAGTAGA‑TTACAACGTGGGCAG‑CACCCCCCTTGCTCCCGGCG‑AGGCACCTTTCACCGCCGTGAACTGGACGCCACTGCATGACAACATGTACGAAGCTGCCg < 2:861009/239‑1 (MQ=255) aCTTTTGCTGCCGATCTGCCGGGCAAAGGCATTACTGTTAATCCAGTTCAGAGCACCATCACTGAAGAAACCTTCCAGACGCTGCTGGTCAGTCGTGCGCTGGAGAAATTAGGTTATACCGTCAACAAACCCAGCGAAGTAGA‑TTACAACGTTGGCTA‑CACCTCGCTTGCTTCCGGCG‑ATGCAACCTTCACCGCCGTGAACTGGACGCCACTGCATGACAACATGTACGAAGCTGCCgg > 1:378586/1‑239 (MQ=255) ccGATCTGCCGGGCAAAGGCATTACTGTTAATCCAGTTCAGAGCACCATCACTGAAGAAACCTTCCAGACGCTGCTGGTCAGTCGTGCGCTGGAGAAATTAGGTTATACCGTCAACAAACCCAGCGAAGTAGA‑TTACAACGTTGGCTA‑CACCTCGCTTGCTTCCGGCG‑ATGCAACCTTCACCGCCGTGAACTGGACGCCACTGCATGACAACATGTACGAAGCTGCCGGTGGCGATAAg > 2:1163841/1‑239 (MQ=255) gggCAAAGGCATTACTGTTAATCCAGTTCAGAGCACCATCACTGAAGAAACCTTCCAGACGCTGCTGGTCAGTCGTGCGCTGGAGAAATTAGGTTATACCGTCAACAAACCCAGCGAAGTAGA‑TTACAACGTTGGCTA‑CACCTCGCTTGCTTCCGGCG‑ATGCAACCTTCACCGCCGTGAACTGGACGCCACTGCATGACAACATGTACGAAGCTGCCGGTGGCGATAAGAAATTTTATc > 1:497174/1‑239 (MQ=255) gCATTACTGTTAATCCAGTTCAGAGCACCATCACTGAAGAAACCTTCCAGACGCTGCTGGTCAGTCGTGCGCTGGAGAAATTAGGTTATACCGTCAACAAACCCAGCGAAGTAGA‑TTACAACGTTGGCTA‑CACCTCGCTTGCTTCCGGCG‑ATGCAACCTTCACCGCCGTGAACTGGACGCCACTGCATGACAACATGTACGAAGCTGCCGGTGGCGATAAGAAATTTTATCGTGAAggg > 2:613228/1‑239 (MQ=255) tACTGTTAATCCAGTTCAGAGCACCATCACTGAAGAAACCTTCCAGACGCTGCTGGTCAGTCGTGCGCTGGAGAAATTAGGTTATACCGTCAACAAACCCAGCGAAGTAGA‑TTACAACGTTGGCTA‑CACCTCGCTTGCTTCCGGCG‑ATGCAACCTTCACCGCCGTGAACTGGACGCCACTGCATGACAACATGTACGAAGCTGCCGGTGGCGATAAGAAATTTTATCGTGAAGGGGTAt > 1:212850/1‑239 (MQ=255) tACTGTTAATCCAGTTCAGAGCACCATCACTGAAGAAACCTTCCAGACGCTGCTGGTCAGTCGTGCGCTGGAGAAATTAGGTTATACCGTCAACAAACCCAGCGAAGTAGA‑TTACAACGTTGGCTA‑CACCTCGCTTGCTTCCGGCG‑ATGCAACCTTCACCGCCGTGAACTGGACGCCACTGCATGACAACATGTACGAAGCTGCCGGTGGCGATAAGAAATTTTATCGTGAAGGGGTAt > 1:503027/1‑239 (MQ=255) ttAATCCAGTTCAGAGCACCATCACTGAAGAAACCTTCCAGACGCTGCTGGTCAGTCGTGCGCTGGAGAAATTAGGTTATACCGTCAACAAACCCAGCGAAGTAGA‑TTACAACGTTGGCTA‑CACCTCGCTTGCTTCCGGCG‑ATGCAACCTTCACCGCCGTGAACTGGACGCCACTGCATGACAACATGTACGAAGCTGCCGGTGGCGATAAGAAATTTTATCGTGAAGGGGTATTTGtt > 1:695857/1‑239 (MQ=255) tCAGAGCACCATCACTGAAGAAACCTTCCAGACGCTGCTGGTCAGTCGTGCGCTGGAGAAATTAGGTTATACCGTCAACAAACCCAGCGAAGTAGA‑TTACAACGTTGGCTA‑CACCTCGCTTGCTTCCGGCG‑ATGCAACCTTCACCGCCGTGAACTGGACGCCACTGCATGACAACATGTACGAAGCTGCCGGTGGCGATAAGAAATTTTATCGTGAAGGGGTATTTGTTAACGgcg > 2:245180/1‑236 (MQ=255) tCAGAGCACCATCACTGAAGAAACCTTCCAGACGCTGCTGGTCAGTCGTGCGCTGGAAAAATTAGGTTATACCGTCAACAAACCCAGCGAAGTAGA‑TTACAACGTTGGCTA‑CACCTCGCTTGCTTCCGGCG‑ATGCAACCTTCACCGCCGTGAACTGGACGCCACTGCATGACAACATGTACGAAGCTGCCGGTGGCGATAAGAAATTTTATCGTGAAGGGGTATTTGTTAACGgcg < 1:245180/236‑1 (MQ=255) cAGACCACCATCACTGAAAAAACCTCCCAGACGCTGCGGGTCAGTCGTGCGCGGGAGAAATTAGGTTATCCCTTCAAAAACCCCAGCGAAGTAGA‑TTACACCGTTGGCTA‑CACCTCGCTTGCTCCCGGCG‑ATGCAACCTTCACCGCCGTGAACTGGACGCCACTGCATGACAACATGTACGAAGCTGCCGGTGGCGATAAGAAATTTTATCGTGAAGGGGTATTTGTTAACGGCGCGGc < 1:527072/239‑1 (MQ=255) agCACCATCACTGAAGAAACCTTCCAGACGCTGCTGGTCAGTCGTGCGCTGGAGAAATTAGGTTATACCGTCAACAAACCCAGCGAAGTAGA‑TTACAACGTTGGCTA‑CACCTCGCTTGCTTCCGGCG‑ATGCAACCTTCACCGCCGTGAACTGGACGCCACTGCATGACAACATGTACGAAGCTGCCGGTGGCGATAAGAAATTTTATCGTGAAGGGGTATTTGTTAACGGCGCGGcaca > 2:1142047/1‑239 (MQ=255) agCACCATCACTGAAGAAACCTTCCAGACGCTGCTGGTCAGTCGTGCGCTGGAGAAATTAGGTTATACCGTCAACAAACCCAGCGAAGTAGA‑TTACAACGTTGGCTA‑CACCTCGCTTGCTTCCGGCG‑ATGCAACCTTCACCGCCGTGAACTGGACGCCACTGCATGACAACATGTACGAAGCTGCCGGTGGCGATAAGAAATTTTATCGTGAAGGGGTATTTGTTAACGGCGCGGcaca > 2:1308810/1‑239 (MQ=255) cATCACTGAAGAAACCTTCCAGACGCTGCTGGTCAGTCGTGCGCTGGAGAAATTAGGTTATACCGTCAACAAACCCAGCGAAGTAGA‑TTACAACGTTGGCTA‑CACCTCGCTTGCTTCCGGCG‑ATGCAACCTTCACCGCCGTGAACTGGACGCCACTGCATGACAACATGTACGAAGCTGCCGGTGGCGATAAGAAATTTTATCGTGAAGGGGTATTTGTTAACGGCGCGGCACAGGGtt > 2:683140/1‑239 (MQ=255) tCACTGAAGAAACCTTCCAGACGCTGCTGGTCAGTCGTGCGCTGGAGAAATTAGGTTATACCGTCAACAAACCCAGCGAAGTAGA‑TTACAACGTTGGCTA‑CACCTCGCTTGCTTCCGGCG‑ATGCAACCTTCACCGCCGTGAACTGGACGCCACTGCATGACAACATGTACGAAGCTGCCGGTGGCGATAAGAAATTTTATCGTGAAGGGGTATTTGTTAACGGCGCGGCACAGGGTTa > 1:1046519/1‑238 (MQ=255) aCCTTCCAGACGCTGCTGGTCAGTCGTGCGCTGGAGAAATTAGGTTATACCGTCAACAAACCCAGCGAAGTAGA‑TTACAACGTTGGCTA‑CACCTCGCTTGCTTCCGGCG‑ATGCAACCTTCACCGCCGTGAACTGGACGCCACTGCATGACAACATGTACGAAGCTGCCGGTGGCGATAAGAAATTTTATCGTGAAGGGGTATTTGTTACCGGCGCGGCACAGGGTTACCTGATCGATaa > 1:104226/1‑239 (MQ=255) aCCTTCCAGACGCTGCTGGTCAGTCGTGCGCTGGAGAAATTAGGTTATACCGTCAACAAACCCAGCGAAGTAGA‑TTACAACGTTGGCTA‑CACCTCGCTTGCTTCCGGCG‑ATGCAACCTTCACCGCCGTGAACTGGACGCCACTGCATGACAACATGTACGAAGCTGCCGGTGGCGATAAGAAATTTTATCGTGAAGGGGTATTTGTTAACGGCGCGGCACAGGGTTACCTGATCGATaa > 1:438598/1‑239 (MQ=255) ttCCAGACGCTGCTGGTCAGTCGTGCGCTGGAGAAATTAGGTTATACCGTCAACAAACCCAGCGAAGTAGA‑TTACAACGTTGGCTA‑CACCTCGCTTGCTTCCGGCG‑ATGCAACCTTCACCGCCGTGAACTGGACGCCACTGCATGACAACATGTACGAAGCTGCCGGTGGCGATAAGAAATTTTATCGTGAAGGGGTATTTGTTAACGGCGCGGCACAGGGTTACCTGATCGATAAGaa > 1:1000371/1‑239 (MQ=255) ccAGACGCTGCTGGTCAGTCGTGCGCTGGAGAAATTAGGTTATACCGTCAACAAACCCAGCGAAGTAGA‑TTACAACGTTGGCTA‑CACCTCGCTTGCTTCCGGCG‑ATGCAACCTTCACCGCCGTGAACTGGACGCCACTGCATGACAACATGTACGAAGCTGCCGGTGGCGATAAGAAATTTTATCGTGAAGGGGTATTTGTTAACGGCGCGGCACAGGGTTACCTGATCGATAAGaaa > 1:793431/1‑238 (MQ=255) gctgctGGTCAGTCGTGCGCTGGAGAAATTAGGTTATACCGTCAACAAACCCAGCGAAGTAGA‑TTACAACGTTGGCTA‑CACCTCGCTTGCTTCCGGCG‑ATGCAACCTTCACCGCCGTGAACTGGACGCCACTGCATGACAACATGTACGAAGCTGCCGGTGGCGATAAGAAATTTTATCGTGAAGGGGTATTTGTTAACGGCGCGGCACAGGGTTACCTGATCGATAAGAAAAccgccg > 2:315120/1‑239 (MQ=255) tgctgGTCAGTCGTGCGCTGGAGAAATTAGGTTATACCGTCAACAAACCCAGCGAAGTAGA‑TTACAACGTTGGCTA‑CACCTCCCTTGCTTCCGGCG‑ATGCAACCTTCACCGCCGTGAACTGGACGCCACTGCATGACAACATGTACGAAGCTGCCGGTGGCGATAAGAAATTTTATCGTGAAGGGGTATTTGTTCACGGCGCGGCACCGGGTTACCTGATCGATAAGCAAACCGCCGa > 2:366645/1‑238 (MQ=255) gcgggTCAGTCGTGCGAGGGAGAAATTAGTTTTCACGGACAACACCCACGGCGAAGAAAA‑TACCAGCGTTGCCTA‑CCCCGCGTTTCCGCCGGCCG‑ACGCACCCTACACCGCCGTGAACTGGACGCCACTGCATGACAACATGTACGAAGCTGCCGGTGGCGATAAGAAATTTTATCGTGAAGGGGTATTTGTTAACGGCGCGGCACAGGGTTACCTGATCGATAAGAAAACCGCCGAc < 2:628787/235‑1 (MQ=255) tCGTGCGCTGGAGAAATTAGGTTATACCGTCAACAAACCCAGCGAAGTAGA‑TTACAACGTTGGCTA‑CACCTCGCTTGCTTCCGGCG‑ATGCAACCTTCACCGCCGTGAACTGGACGCCACTGCATGACAACATGTACGAAGCTGCCGGTGGCGATAAGAAATTTTATCGTGAAGGGGTATTTGTTAATGGCGCGGCACAGGGTTACCTGATCGATAAGAAAACCGCCGACCAGAACaaaa > 2:439238/1‑239 (MQ=255) tCGTGCGCTGGAGAAATTAGGTTATACCGTCAACAAACCCAGCGAAGTAGA‑TTACAACGTTGGCTA‑CACCTCGCTTGCTTCCGGCG‑ATGCAACCTTCACCGCCGTGAACTGGACGCCACTGCATGACAACATGTACGAAGCTGCCGGTGGCGATAAGAAATTTTATCGTGAAGGGGTATTTGTTAACGGCGCGGCACAGGGTTACCTGATCGATAAGAAAACCGCCGACCAGTACaaaa > 2:1338707/1‑239 (MQ=255) cgcTGGAGAAATTAGGTTATACCGTCAACAAACCCAGCGAAGTAGA‑TTACAACGTTGGCTA‑CACCTCGCTTGCTTCCGGCG‑ATGCAACCTTCACCGCCGTGAACTGGACGCCACTGCATGACAACATGTACGAAGCTGCCGGTGGCGATAAGAAATTTTATCGTGAAGGGGTATTTGTTAACGGCGCGGCACAGGGTTACCTGATCGATAAGAAAACCGCCGACCAGTACAAAATCAcc > 1:989990/1‑239 (MQ=255) cgcTGGAGAAATTAGGTTATACCGTCAACAAACCCAGCGAAGTAGA‑TTACAACGTTGGCTA‑CACCTCGCTTGCTTCCGGCG‑ATGCAACCTTCACCGCCGTGAACTGGACGCCACTGCATGACAACATGTACGAAGCTGCCGGTGGCGATAAGAAATTTTATCGTGAAGGGGTATTTGTTAACGGCGCGGCACAGGGTTACCTGATCGATAAGAAAACCGCCGACCAGTACAAAATCAcc > 2:199702/1‑239 (MQ=255) gagaAATTAGGTTATACCGTCAACAAACCCAGCGAAGTAGA‑TTACAACGTTGGCTA‑CACCTCGCTTGCTTCCGGCG‑ATGCAACCTTCACCGCCGTGAACTGGACGCCACTGCATGACAACATGTACGAAGCTGCCGGTGGCGATAAGAAATTTTATCGTGAAGGGGTATTTGTTAACGGCGCGGCACAGGGTTACCTGATCGATAAGAAAACCGCCGACCAGTACAAAATCACCAACa > 2:555183/1‑238 (MQ=255) aaTTAGGTTATACCGTCAAAAACCCCAGGGAAGTAAA‑TTAAACCGTT‑GCTACCCCCCCCCTTGCTCCCGGCG‑ATCCACCCTTCCCCGCCGTGAACTGGACGCCACTGCATGACAACATGTACGAAGCTGCCGGTGGCGATAAGAAATTTTATCGTGAAGGGGTATTTGTTAACGGCGCGGCACAGGGTTACCTGATCGATAAGAAAACCGCCGACCAGTACAAAATCACCAACATCGca < 1:882597/239‑1 (MQ=255) gTTATACCGTCAACAAACCCAGCGAAGTAGA‑TTACAACGTTGGCTA‑CACCTCGCTTGCTTCCGGCG‑ATGCAACCTTCACCGCCGTGAACTGGACGCCACTGCATGACAACATGTACGAAGCTGCCGGTGGCGATAAGAAATTTTATCGTGAAGGGGTa > 2:987459/1‑158 (MQ=255) gTTATACCGTCAACAAACCCAGCGAAGTAGA‑TTACAACGTTGGCTA‑CACCTCGCTTGCTTCCGGCG‑ATGCAACCTTCACCGCCGTGAACTGGACGCCACTGCATGACAACATGTACGAAGCTGCCGGTGGCGATAAGAAATTTTATCGTGAAGGGGTa < 1:987459/158‑1 (MQ=255) taCCGTCAACAAACCCAGCGAAGTAGA‑TTACAACGTTGGCTA‑CACCTCGCTTGCTTCCGGCG‑ATGCAACCTTCACCGCCGTGAACTGGACGCCACTGCa > 1:309689/1‑99 (MQ=255) caacaaACCCAGCGAAGTAGA‑TTACAACGTTGGCTA‑CACCTCGCTTGCTTCCGGCG‑ATGCAACCTTCACCGCCGTGAACTGGACGCCACTGCATGACAACATGTACGAAGCTGCCGGTGGCGATAAGAAATTTTATCGTGAAGGGGTATTTGTTAACGGCGCGGCACAGGGTTACCTGATCGATAAGAAAACCGCCGACCAGTACAAAATCACCAACATCGCACAACTGAAAGATCCGa > 2:1130795/1‑239 (MQ=255) caacaaACCCAGCGAAGTAGA‑TTACAACGTTGGCTA‑CACCTCGCTTGCTTCCGGCG‑ATGCAACCTTCACCGCCGTGAACTGGACGCCACTGCATGACAACATGTACGAAGCTGCCGGTGGCGATAAGAAATTTTATCGTGAAGGGGTATTTGTTAACGGCGCGGCACAGGGTTACCTGATCGATAAGAAAACCGCCGACCAGTACAAAATCACCAACATCGCACAACTGAAAGATCCGa > 1:976007/1‑239 (MQ=255) caacaaACCCAGCGAAGTAGA‑TTACAACGTTGGCTA‑CACCTCGCTTGCTTCCGGCG‑ATGCAACCTTCACCGCCGTGAACTGGACGCCACTGCATGACAACATGTACGAAGCTGCCGGTGGCGATAAGAAATTTTATCGTGAAGGGGTATTTGTTAACGGCGCGGCACAGGGTTACCTGATCGATAAGAAAACCGCCGACCAGTACAAAATCACCAACATCGCACAACTGAAAGATCCGa > 2:696762/1‑239 (MQ=255) acaacccccAGCAAGGTAAA‑TTACACCTGTGTCTA‑CCCCCCGTTTTCTCCCGGCG‑ATCCACCCTCCCCCGCCGTGAACTGGACGCCACTGCATGACAACATGTACGAAGCTGCCGGTGGCGATAAGAAATTTTATCGTGAAGGGGTATTTGTTAACGGCGCGGCACAGGGTTACCTGATCGATAAGAAAACCGCCGACCAGTACAAAATCACCAACATCGCACAACTGAAAGATCCGaa < 1:122829/233‑1 (MQ=255) aacaacCCCAGCAAAG‑AGAGCTAAAACGTTGGCTC‑CACCGCGCTTTCTGCCGGCG‑AGGAACCCTCCACCGCCGTGAACTGGACGCCACTGCATGACAACATGTACGAAGCTGCCGGTGGCGATAAGAAATTTTATCGTGAAGGGGTATTTGTTAACGGCGCGGCACAGGGTTACCTGATCGATAAGAAAACCGCCGACCAGTACAAAATCACCAACATCGCACAACTGAAAGATCCGaa < 1:1318864/239‑1 (MQ=255) acaaACCCCGGGAAGTAAATTTACAACTTTGCCCA‑CCCCCCGTTTCCTCCCGGGA‑ATGAACCCTCCCCCGCCGTGAACTGGACGCCACTGCATGACAACATGTACGAAGCTGCCGGTGGCGATAAGAAATTTTATCGTGAAGGGGTATTTGTTAACGGCGCGGCACAGGGTTACCTGATCGATAAGAAAACCGCCGACCAGTACAAAATCACCAACATCGCACAACTGAAAGATCCGaa < 1:1075542/239‑1 (MQ=255) gCGAAGTAGA‑TTACAACGTTGGCTA‑CACCTCGCTTGCTTCCGGCG‑ATGCAACCTTCACCGCCGTGAACTGGACGCCACTGCATGACAACATGTACGAAGCTGCCGGTGGCGATAAGAAATTTTATCGTGAAGGGGTATTTGTTAACGGCGCGGCACAGGGTTACCTGATCGATAAGAAAACCGCCGACCAGTACAAAATCACCAACATCGCACAACTGaa > 1:1004136/1‑220 (MQ=255) gCGAAGTAGA‑TTACAACGTTGGCTA‑CACCTCGCTTGCTTCCGGCG‑ATGCAACCTTCACCGCCGTGAACTGGACGCCACTGCATGACAACATGTACGAAGCTGCCGGTGGCGATAAGAAATTTTATCGTGAAGGGGTATTTGTTAACGGCGCGGCACAGGGTTACCTGATCGATAAGAAAACCGCCGACCAGTACAAAATCACCAACATCGCACAACTGAAAGATCCGAAGATCGCCaa > 2:204065/1‑238 (MQ=255) gCGAAGTAGA‑TTACAACGTTGGCTA‑CACCTCGCTTGCTTCCGGCG‑ATGCAACCTTCACCGCCGTGAACTGGACGCCACTGCATGACAACATGTACGAAGCTGCCGGTGGCGATAAGAAATTTTATCGTGAAGGGGTATTTGTTAACGGCGCGGCACAGGGTTACCTGATCGATAAGAAAACCGCCGACCAGTACAAAATCACCAACATCGCACAACTGAAAGATCCGAAGATCGCCaa > 1:217509/1‑238 (MQ=255) gCGAAGTAGA‑TTACAACGTTGGCTA‑CACCTCGCTTGCTTCCGGCG‑ATGCAACCTTCACCGCCGTGAACTGGACGCCACTGCATGACAACATGTACGAAGCTGCCGGTGGCGATAAGAAATTTTATCGTGAAGGGGTATTTGTTAACGGCGCGGCACAGGGTTACCTGATCGATAAGAAAACCGCCGACCAGTACAAAATCACCAACATCGCACAACTGAAAGATCCGAAGATCGCCaa > 2:779408/1‑238 (MQ=255) tAGT‑TTACAACTTGGGCTA‑CCCCCCGCTTGCTTCCGGCG‑AGGCACCTTTCCCCGCCGTGAACTGGACGCCACTGCATGACAACATGTACGAAGCTGCCGGTGGCGATAAGAAATTTTATCGTGAAGGGGTATTTGTTAACGGCGCGGc < 2:786534/148‑1 (MQ=255) tAGA‑TTACAACGTTGGCTA‑CACCTCGCTTGCTTCCGGCG‑ATGCAACCTTCACCGCCGTGAACTGGACGCCACTGCATGACAACATGTACGAAGCTGCCGGTGGCGATAAGAAATTTTATCGTGAAGGGGTATTTGTTAACGGCGCGGCACAGGGTTACCTGATCGATAAGAAAACCGCCGACCAGTACAAAATCACCAACATCGCACAACTGAAAGATCCGAAGATCGCCAAACTGTTc > 2:130516/1‑239 (MQ=255) aaCGTTGGCTA‑CACCTCGCTTGCTTCCGGCG‑ATGCAACCTTCACCGCCGTGAACTGGACGCCACTGCATGACAACATGTACGAAGCTGCCGGTGGCGATAAGAAATTTTATCGTGAAGGGGTATTTGTTAACGGCGCGGCACAGGGTTACCTGATCGATAAGAAAACCGCCGACCAGTACAAAATCACCAACATCGCACAACTGAAAGATCCGAAGATCGCCAAACTGTTCGATACCa > 1:338041/1‑238 (MQ=255) cGTTGGCTA‑CACCTCGCTTGCTTCCGGCG‑ATGCAACCTTCACCGCCGTGAACTGGACGCCACTGCATGACAACATGTACGAAGCTGCCGGTGGCGATAAGAAATTTTATCGTGAAGGGGTATTTGTTAACGGCGCGGCACAGGGTTAc > 1:786534/1‑148 (MQ=255) cGTTGGCTA‑CACCTCGCTTGCTTCCGGCG‑ATGCAACCTTCACCGCCGTGAACTGGACGCCACTGCATGACAACATGTACGAAGCTGCCGGTGGCGATAAGAAATTTTATCGTGAAGGGGTATTTGTTAACGGCGCGGCACAGGGTTACCTGATCGATAAGAAAACCGCCGACCAGTACAAAATCACCAACATCGCACAACTGAAAGATACGAAGATCGCCAAACTGTTCGATAcacacg > 1:1297865/1‑234 (MQ=255) tGGCTA‑CACCTCGCTTGCTTCCGGCG‑ATGCAACCTTCACCGCCGTGAACTGGACGCCACTGCATGACAACATGTACGAAGCTGCCGGTGGCGATAAGAAATTTTATCGTGAAGGGGTATTTGTTAACGGCGCGGCACAGGGTTACCTGATCGATAAGACAACCGCCGACCAGTACAAAATCACCAACATCGCACAACTGCAAGATCCGAAGATCGCCAAACTGTCCGATACCAACGGCg > 2:492371/1‑239 (MQ=255) tGGCTA‑CACCTCGCTTGCTTCCGGCG‑ATGCAACCTTCACCGCCGTGAACTGGACGCCACTGCATGACAACATGTACGAAGCTGCCGGTGGCGATAAGAAATTTTATCGTGAAGGGGTATTTGTTAACGGCGCGGCACAGGGTTACCTGATCGATAAGAAAACCGCCGACCAGTACAAAATCACCAACATCGCACAACTGAAAGATCCGAAGATCGACAAACTGTTCGATACCAACGGCg > 1:152665/1‑239 (MQ=255) ggCTA‑CACCTCGCTTGCTTCCGGCG‑ATGCAACCTTCACCGCCGTGAACTGGACGCCACTGCATGACAACATGTACGAAGCTGCCGGTGGCGATAAGAAATTTTATCGTGAAGGGGTATTTGTTAACGGCGCGGCACAGGGTTACCTGATCGATAAGAAAACCGCCGACCAGTACAAAATCACCAACATCGCACAACTGAAAGATCCGAAGATCGCCAAACTGTTCGATACCAACGGCg > 1:4581/1‑238 (MQ=255) cacCTCGCTTGCTTCCGGCG‑ATGCAACCTTCACCGCCGTGAACTGGACGCCACTGCATGACAACATGTACGAAGCTGCCGGTGGCGATAAGAAATTTTATCGTGAAGGGGTATTTGTTAACGGCGCGGCACAGGGTTACCTGATCGATAAGAAAACCGCCGACCAGTACAAAATCACCAACATCGCACAACTGAAAGATCCGAAGATCGCCAAACTGTTCGATACCAACGGCGACGGaa > 2:449164/1‑239 (MQ=255) acCTCGCTTGCTTCCGGCG‑ATGCAACCTTCACCGCCGTGAACTGGACGCCACTGCATGACAACATGTACGAAGCTGCCGGTGGCGATAAGAAATTTTATCGTGAAGGGGTATTTGTTAACGGCGCGGCACAGGGTTACCTGATCGATAAGAAAACCGCCGACCAGTACAAAATCACCAACATCGCACAACTGAAAGATCCGAAGATCGCCAAACTGTTCGATACCACAGGCGACGGaaa > 2:784702/1‑239 (MQ=255) ccTCGCTTGCTTCCGGCG‑ATGCAACCTTCACCGCCGTGAACTGGACGCCACTGCATGACAACATGTACGAAGCTGCCGGTGGCGATAAGAAATTTTATCGTGAAGGGGTATTTGTTAACGGCGCGGc < 1:767450/127‑1 (MQ=255) ccTCGCTTGCTTCCGGCG‑ATGCAACCTTCACCGCCGTGAACTGGACGCCACTGCATGACAACATGTACGAAGCTGCCGGTGGCGATAAGAAATTTTATCGTGAAGGGGTATTTGTTAACGGCGCGGc > 2:767450/1‑127 (MQ=255) cTCGCTTGCTTCCGGCG‑ATGCAACCTTCACCGCCGTGAACTGGACGCCACTGCATGACAACATGTACGAAGCTGCCGGTGGCGATAAGAAATTTTATCGTGAAGGGGTATTTGTTAACGGCGCGGCACAGGGTTACCTGATCGATAAGAAAACCGCCGACCAGTACAAAATCACCAACATCGCACAACTGAAAGATCCGAAGATCGCCAAACTGTTCGATACCAACGGCGACGGaaaa > 1:433230/1‑238 (MQ=255) gcttgcttCCGGCG‑ATGCAACCTTCACCGCCGTGAACTGGACGCCACTGCATGACAACATGTACGAAGCTGCCGGTGGCGATAAGAAATTTTATCGTGAAGGGGTATTTGTTAACGGCGCGGCACAGGGTTACCTGATCGATAAGAAAACCGCCGACCAGTACAAAATCACCAACATCGCACAACTGAAAGATCCGAAGATCGCCAAACTGTTCGATACCAAc > 1:1094193/1‑223 (MQ=255) tCCGGCG‑ATGCAACCTTCACCGCCGTGAACTGGACGCCACTGCATGACAACATGTACGAAGCTGCCGGTGGCGATAAGAAATTTTATCGTGAAGGGGTATTTGTTAACGGCGCGGCACAGGGTTACCTGATCGATAAGAAAACCGCCGACCAGTACAAAATCACCAACATCGCAAAACTGAAAGATCCGAAGATCGCCAAACTGTTCGATACCAACGGCGACGGAAAAGCGGATTTaa > 2:341191/1‑238 (MQ=255) ccGGCGTATCCAACCTTCCCCGCCGTGACCTGGACGCCACTGCATGACAACATGTACGAAGCTGCCGGTGGCGATAAGAAATTTTATCGTGAAGGGGTATTTTTTAACGGCGCGGCACAGGGTTACCTGATCGATAAGAAAACCGCCGACCAGTACAAAATCACCAACATCGCACAACTGAAAGATCCGAAGATCGCCAAACTGTTCGATACCAACGGCGACGGAAAAGCGGATTTAAc < 2:217509/239‑1 (MQ=255) tGCAACCTTCACCGCCGTGAACTGGACGCCACTGCATGACAACATGTACGAAGCTGCCGGTGGCGATAAGAAATTTTATCGTGAAGGGGTATTTGTTAACGGCGCGGCACAGGGTTACCTGATCGATAAGAAAACCGCCGACCAGTACAAAATCACCAACATCGCACAACTGAAAGATCCGAAGATCGCCAAACTGTTCGATACCAACGGCGACGGAAAAGCGGATTTAACCGGTTGTa > 2:654265/1‑239 (MQ=255) | GCGACATAGCGTACTTTTTGCGACAGCGTTTGCCACGCTTATCTCTACACAAACTTTTGCTGCCGATCTGCCGGGCAAAGGCATTACTGTTAATCCAGTTCAGAGCACCATCACTGAAGAAACCTTCCAGACGCTGCTGGTCAGTCGTGCGCTGGAGAAATTAGGTTATACCGTCAACAAACCCAGCGAAGTAGA‑TTACAACGTTGGCTA‑CACCTCGCTTGCTTCCGGCG‑ATGCAACCTTCACCGCCGTGAACTGGACGCCACTGCATGACAACATGTACGAAGCTGCCGGTGGCGATAAGAAATTTTATCGTGAAGGGGTATTTGTTAACGGCGCGGCACAGGGTTACCTGATCGATAAGAAAACCGCCGACCAGTACAAAATCACCAACATCGCACAACTGAAAGATCCGAAGATCGCCAAACTGTTCGATACCAACGGCGACGGAAAAGCGGATTTAACCGGTTGTA > NC_000913/2807134‑2807603 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |