Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,206,487 C→A 20.5% Q9K (CAG→AAG)  ttdA → L‑tartrate dehydratase, alpha subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,206,4870CA20.5% 202.0 / 18.7 101Q9K (CAG→AAG) ttdAL‑tartrate dehydratase, alpha subunit
Reads supporting (aligned to +/- strand):  ref base C (14/61);  new base A (18/3);  total (32/69)
Fisher's exact test for biased strand distribution p-value = 2.60e-08
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 105 total aligned reads displayed.

ATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTAC‑ATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGC‑GGTGAATTTTTCCAGACAAATACAAAAA‑CTGG‑A‑GTTG‑CC‑ATG‑AT‑GAGCGAAAGTAATAAGCAACAGGCAGTG‑AATAAGTTGACAGAG‑ATTGTCGCTAAC‑TTT‑ACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCTTCCC  >  NC_000913/3206255‑3206725
                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                  
aTCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTAC‑ATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGC‑GGTGAATTTTTCCAGACAAATACAAAAA‑CTGG‑A‑GTTG‑CC‑ATG‑AT‑GAGCGAAAGTAATAAGCAACAgg                                                                                                                                                                                                                                                 <  1:835962/236‑1 (MQ=255)
aTCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTAC‑ATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGC‑GGTGAATTTTTCCAGACAAATACAAAAA‑CTGG‑A‑GTTG‑CC‑ATG‑AT‑GAGCGAAAGTAATAAGCAACAgg                                                                                                                                                                                                                                                 <  2:57842/236‑1 (MQ=255)
aTCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTAC‑ATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGC‑GGTGAATTTTTCCAGACAAATACAAAAA‑CTGG‑A‑GTTG‑CC‑ATG‑AT‑GAGCGAAAGTAATAAGCAACAgg                                                                                                                                                                                                                                                 <  1:719283/236‑1 (MQ=255)
aTCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTAC‑ATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGC‑GGTGAATTTTTCCAGACAAATACAAAAA‑CTGG‑A‑GTTG‑CC‑ATG‑AT‑GAGCGAAAGTAATAAGCAACAgg                                                                                                                                                                                                                                                 <  2:356970/236‑1 (MQ=255)
aTCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTAC‑ATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGC‑GGTGAATTTTTCCAGACAAATACAAAAA‑CTGG‑A‑GTTG‑CC‑ATG‑AT‑GAGCGAAAGTAATAAGCAACAgg                                                                                                                                                                                                                                                 <  1:132617/236‑1 (MQ=255)
aTCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTAC‑ATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGC‑GGTGAATTTTTCCAGACAAATACAAAAA‑CTGG‑A‑GTTG‑CC‑ATG‑AT‑GAGCGAAAGTAATAAGCAACAGGc                                                                                                                                                                                                                                                <  1:738001/237‑1 (MQ=255)
  cGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGAGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTAC‑ATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGC‑GGTGAATTTTTTTAGACAAAAACAAAAA‑CAGG‑A‑GTTG‑CC‑ATG‑GC‑GAGGGAAAGAAATAAACAACAGAAAGTg                                                                                                                                                                                                                                            >  1:251238/1‑239 (MQ=255)
     tAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTAC‑ATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGC‑GGTGAATTTTTCCAGACAAATACAAAAA‑CTGG‑A‑GTTG‑CC‑ATG‑AT‑GAGCGAAAGTAATAAGCAACAGGCAGTG‑aa                                                                                                                                                                                                                                         <  1:722731/238‑1 (MQ=255)
     tAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTAC‑ATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGC‑GGTGAATTTTTCCAGACAAATACAAAAA‑CTGG‑A‑GTTG‑CC‑ATG‑AT‑GAGCGAAAGTAATAAGCAACAGGCAGTG‑aa                                                                                                                                                                                                                                         <  2:688796/238‑1 (MQ=255)
     tAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTAC‑ATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGC‑GGTGAATTTTTCCAGACAAATACAAAAA‑CTGG‑A‑GTTG‑CC‑ATG‑AT‑GAGCGAAAGTAATAAGCAACAGGCAGTG‑aa                                                                                                                                                                                                                                         <  2:1119944/238‑1 (MQ=255)
      aaTTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTAC‑ATATTCACGGTGGCAAAAAATATAAAACCGCATTTTTAGTGGTAGTTTGTGGC‑GGTGAATTTTTCCAGACAAATACAAAAA‑CTGT‑A‑GTTG‑CC‑ATG‑AT‑GAGCGAAAGTAATAAGCAAAAGGCAGTG‑aaga                                                                                                                                                                                                                                       <  2:151113/239‑3 (MQ=255)
        ttATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTAC‑ATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGC‑GGTGAATTTTTCCAGACAAATACAAAAA‑CTGG‑A‑GTTG‑CC‑ATG‑AT‑GAGCGAAAGTAATAAGCAACAGGCAGTG‑AATAAg                                                                                                                                                                                                                                     <  1:76192/239‑1 (MQ=255)
         tATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTAC‑ATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGC‑GGTGAATTTTTCCAGACAAATACAAAAA‑CTGG‑A‑GTTG‑CC‑ATG‑AT‑GAGCGAAAGTAATAAGCAACAGGCAGTG‑AATAAGt                                                                                                                                                                                                                                    <  1:1011294/239‑1 (MQ=255)
              gCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTAC‑ATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGC‑GGTGAATTTTTCCAGACAAATACAAAAA‑CTGG‑A‑GTTG‑CC‑ATG‑AT‑GAGCGAAAGTAATAAGCAACAGGCAGTG‑AATAAGTTGACa                                                                                                                                                                                                                               <  2:193477/239‑1 (MQ=255)
                   tgtgATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTAC‑ATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGC‑GGTGAATTTTTCCAGACAAATACAAAAA‑CTGG‑A‑GTTG‑CC‑ATG‑AT‑GAGCGAAAGTAATAAGCAACAGGCAGTG‑AATAAGTTGACAgag‑a                                                                                                                                                                                                                          <  1:1030916/238‑1 (MQ=255)
                          aGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTAC‑ATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGC‑GGTGAATTTTTCCAGACAAATACAAAAA‑CTGG‑A‑GTTG‑CC‑ATG‑AT‑GAGCGAAAGTAATAAGCAACAGGCAGTG‑AATAAGTTGACAGAG‑ATTGTCGCt                                                                                                                                                                                                                  <  2:237923/239‑1 (MQ=255)
                            cgcAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTAC‑ATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGC‑GGTGAAATTTTTCAGAAAAATAAAAAAA‑AATG‑G‑GGTG‑GC‑ATG‑AT‑TAGCGAAAGGAATAAACAAAAAGAAGGG‑AATAAAATGACAGAG‑Atttttgccaa                                                                                                                                                                                                                >  1:983174/1‑231 (MQ=255)
                               aGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTAC‑ATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGC‑GGTGGATTTTTTCAGAAAAAAACAAAAA‑CTTG‑A‑GTTG‑CC‑CTG‑AT‑GAGGGGAAGTAATAAACAAAAGGCAGTG‑AATAAATTGACAGAG‑ATTGttggttaatt                                                                                                                                                                                                             >  1:503738/1‑230 (MQ=255)
                                 tgtATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTAC‑ATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGC‑GGTGGATTTTTTCAGACAAAAAAAAAAA‑ATGG‑A‑GTTG‑GC‑ACG‑AT‑GAGCGGGAGGAATAAGTAACAAGGAGAG‑AAAAAATTGGCAGAG‑ATTTTCTCTTAA‑TTT‑a                                                                                                                                                                                                         >  1:1225525/1‑239 (MQ=255)
                                   tATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTAC‑ATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGC‑GGTGAATTTTTCCAGACAAATACAAAAA‑CTGG‑A‑GTTG‑CC‑ATG‑AT‑GAGCGAAAGTAATAAGCAACAGGCAGTG‑AATAAGTTGACAGAG‑ATTGTCGCTAAC‑TTT‑Acc                                                                                                                                                                                                       <  1:824393/239‑1 (MQ=255)
                                       tCGAAAAACTTTGCCATTCCCTGCCTTGGCGCAATTTTCATTCTTATTTTCGCGTTGATTCACAAACTGTTATTTAC‑ATATTCAGGGTGGCAAAAAATAAAAACCCACATTTTTAGTGGTAGTTTGTGGC‑GGTGAATTTTTCCAGACAAATACAAAAA‑CTGG‑A‑GTTG‑CC‑ATG‑AT‑GAGCGAAAGTAAAAAGCAACAGGCAGTG‑AATAAGTTGACAGAG‑TTTGTCGCTAAT‑TTT‑ACCGCCa                                                                                                                                                                                                   <  2:1225525/239‑1 (MQ=255)
                                       tCGAAAAACATTGCCATCCCCTGCTCTGGCGCAATATTCATTCTTATTGTCGCGTTGATTCACGAACTGTTAATTAC‑ATATTCACGGTGGCAAAAAATAAAAACCCACATTTTTAGGGGTAGTTTGTGGC‑GGTGAATTTTTCCAGACAAATACAAAAA‑CGGG‑A‑GTTG‑CC‑ATG‑AT‑GAGCAAAAGTAATAAGCAACAGGCAGTG‑AATAAGTTGACAGAG‑ATTGTCGCTAAC‑TTT‑ACCGCCa                                                                                                                                                                                                   <  2:1128342/239‑1 (MQ=255)
                                             aaCATTGCCATTCCCTGCTCTGGCGC‑ATATTCATTCTTATTTTCGCGTTGATTCACGTACTGTTAATTAC‑ATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGC‑GGTGGAGTTTTTCCGTGAAATACAAAAA‑CCAG‑A‑GCTG‑GT‑ATG‑GT‑GATAGGGCGTAATAAGCAACAAGGAGAG‑GGTAAAATGAAAGGG‑GTTGTGCCTAAG‑TTT‑ACCGCCATGAtgta                                                                                                                                                                                            >  2:779950/1‑236 (MQ=255)
                                               cATTGCCATCCCTTGCTCTGGCGAAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTAC‑ATATTCACGGTGGAAAAAAATATAAACCCACATTTTTAGGGGGAGTTTGGGGC‑GGTAAATTTTTCCAGACAAATACAAAAA‑CGGG‑A‑GTTG‑CC‑ATG‑AT‑GAGCGAAAGTAATAAGCAACAGGCAGTG‑AATAAGTTGACAGAG‑ATTGTCGCTAAC‑TTT‑ACCGCCATGATTTCt                                                                                                                                                                                           <  1:696702/239‑1 (MQ=255)
                                                     cacccctttctctGGCGCATTATATATTCTGATTACCGAGTTGAACAACGAACTGTTAAATGC‑ATATGCCGGGGGGAAAAAAATATAAATCAACATTTGTAGTGGGATGTTGGGGG‑GGTGATTTTAACAAAAAAAAAAAAAAAA‑CAGG‑A‑GTTT‑CC‑ATA‑AG‑GCGCTAAAAAAATAAGCAACAGACGGAA‑AAAAAGTATACAGAG‑TTTGCCGTTAAC‑TTT‑ACCGCCATGATTTCTACCCGa                                                                                                                                                                                     <  2:318866/230‑1 (MQ=255)
                                                     cATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGTTTCACGAACTGTTAATTAC‑ATATTCACGGTGGCAAAAAATAAAAAACCACATTTTTAGTGGTAGTTTGTGGC‑GGTGAATTTTTCCAAACAAATACAAAAA‑TTGG‑A‑GTTG‑CC‑ATG‑AT‑AAGCGAAAGTAATAAGCAACAGGCAGTA‑AATAAGTGGAAAGAG‑ATTGTCCCTAAC‑TTT‑ACCGCCATGATTTCTACCCGa                                                                                                                                                                                     <  1:95797/239‑1 (MQ=255)
                                                         cccTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTAC‑ATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGC‑GGTGAAATTTTTCAGACAAATACAAAAA‑CAGG‑A‑GGTG‑CC‑CTG‑AT‑GAGAGAAAAAAAAAAACAAAACACAGGG‑GAAAAATTTAAAAGG‑AATTTAGCTCAC‑TTT‑ACCGCCCACATTTTTTCCCGaaag                                                                                                                                                                                  >  2:634911/1‑236 (MQ=255)
                                                              ctctGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTAC‑ATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGC‑GGTGAATTTTTTCAGGAAAAAACAAAAA‑CTTG‑C‑GTTG‑CC‑ACT‑CT‑TTGTGCAAGGAATAAGCAACAGGCCGAG‑GATAAGATGGCAGAG‑ATTTTCGGCAAA‑TTT‑ACCGCCATGATTTTTACCCGAAAGCCtgtt                                                                                                                                                                            >  1:156371/1‑237 (MQ=255)
                                                                  ggCGAAATATTATTACTTATTACGGGGTTGATACACGAAATTTAAATTCA‑ATATTCTGGGTGGAAAAAAAGATAAAACCATTTTTTAATTGGGAGTTTG‑GGG‑GGGGAATTTTTACGAAAAAAAGCAACAA‑GTGGAA‑GTTT‑CC‑AAG‑AG‑GAGAGAAGTTAAAAGGCAACAGGTAGGA‑AAAAGGTAGACGGAA‑GTTGTCGTAATC‑TTT‑ACCGCCATGATTTCTACCCGAATGCCTGATGAc                                                                                                                                                                         <  2:1329746/238‑1 (MQ=255)
                                                                    cgcAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTAC‑ATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGC‑GGTGAATTTTTTCCGACAAATACAAAAA‑ATGG‑A‑GGTG‑GC‑CTG‑AT‑GAGAGAAAGTAATAAAAAAAAAGCAGGG‑AATAAATTTAAAGAG‑AATTTCGCCAAA‑TTT‑ACCGCCATGATTTCTACCCGAATGCCCGATGAAgtg                                                                                                                                                                      >  1:507303/1‑239 (MQ=255)
                                                                    cgaaaTATTCATTCTTATTTTCGGTTTGATTCACGAACTGTTAATTAC‑ATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGC‑GGTAAATTTTTCCAGACAAATACAAAAA‑CTGG‑A‑GTTG‑CC‑ATG‑AT‑GAGCGAAAGTAATAAGCAACAGGCAGTG‑AATAAGTTGACAGAG‑ATTGTCGCTAAC‑TTT‑ACCGCCATGATTTCTACCCGAATGCCTGATGACgt                                                                                                                                                                       <  1:1272623/235‑1 (MQ=255)
                                                                     gaaaTATTCATCTTTATTTCGTCGTTGATACACGAA‑TTTTAAATACAATATTCACGGTGACAAAAAATATAAAACCCCATTTTAAGTGGTAGTTCGCGGC‑GGTAATTTTTCCCAAACAAATACAAAAA‑CGGG‑A‑GTTC‑CC‑CTG‑AT‑GAGCGAAAGAAATAAGCACCAGGCAGTG‑AATAAGTTGCCAGAA‑ATTGTCGCTAAC‑TTT‑ACCGCCATGATTTCTACCCGAATGCCTGATGACgtg                                                                                                                                                                      <  2:561957/236‑1 (MQ=255)
                                                                         ttttcattcTTATTTTCGCTTTTATACACGAACTTTTAATTAC‑ATATTCACGGTGGAAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGC‑GGTAAATTTTTCCAAACAAATACAAAAA‑CGGG‑A‑GTGG‑CC‑ATG‑AT‑GAGCGAAAGTAATAAGCAACGGGCGGTG‑AATAAGTTGACAGAG‑ATTGTCGCTAAC‑TTT‑ACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGa                                                                                                                                                                 <  2:28948/237‑1 (MQ=255)
                                                                         tatgtattcTTATTGCCTCGTTGATTCACGAACTGTTAATTAC‑ATATTCACGGTGGCAAAAAATATAAAACCGCATTTTTAGGGGTAGTTTGGGGC‑GGTGAATTTTTCCAGACAAATAAAAAAA‑CTGG‑A‑GTTG‑CC‑TTG‑AG‑GAGCGAAAGTAATAAGCAACAGGCAGTA‑AATAAGTTGACAGGG‑ATGGCCGTTACC‑TTT‑TCCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGa                                                                                                                                                                 <  1:1342860/234‑1 (MQ=255)
                                                                         tatTCATTTTTATTTTCGCGTTGATTCACGAATTGTTAATTAC‑ATATTCAGGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGC‑GGTGAATTTTTCCAGAAAAATACAAAAA‑CTGG‑A‑GTGG‑CC‑ATG‑AT‑GAGCGAAAGTAATAAGAAACAGGCAGTG‑AATAAGTTGACAGAG‑ATTGTCGCTAAC‑TTT‑ACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGa                                                                                                                                                                 <  1:7648/239‑1 (MQ=255)
                                                                         tatTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTAC‑ATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGC‑GGTGAATTTTTCCAGACAAATACAAAAA‑CTGG‑A‑GTTG‑CC‑ATT‑AT‑GAGCGAAAGTAATAAGCAACAGGCAGTG‑AATAAGTTGACAGAG‑ATTGTCGCTAAC‑TTT‑Acc                                                                                                                                                                                                       <  1:735171/201‑1 (MQ=255)
                                                                             cattcTTATTTTCGCGTTGATTCACGAACTGTTAATTAC‑ATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGC‑GGTGAAATTTTTCCGACAAATACAAAAA‑ATGG‑A‑GTTG‑CC‑CTG‑AT‑GAGCGGAAGGAATAAAGAACAAGCAGTG‑AAAAAGATGACAGAG‑AATGTCGCCAAA‑TTT‑AACGCCATGATTTTTAACCGAATGCCTGATGACGTGGGGGGTaaa                                                                                                                                                             >  1:561957/1‑239 (MQ=255)
                                                                              attcTTATTTTCGCGTTGATTCACGAACTGTTAATTAC‑ATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGC‑GGTGAATTTTTTCAAACAAAAACAAAAA‑ATGG‑A‑GGTT‑CC‑CTG‑AT‑GAGGGAAAATAATAAGCAAAAAGCAAGG‑AATAAATTGTCAGAG‑AATGTCGCTAAC‑TTT‑AACCCCATGCTTTTTACCCGAAAGCCCGGTGACGggggggggaaa                                                                                                                                                             >  2:1272623/1‑233 (MQ=255)
                                                                                  ttATTTTCGCGTTGATTCACGAACTGTTAATTAC‑ATATTCACGGTGGAAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGC‑GGTGAATTTTTCCAGACAAATACAAAAA‑CTGG‑A‑GTTG‑CC‑ATG‑AT‑GAGCGAAAGTAATAAGCAACAGGCAGTG‑AATAAGTTGACAGAG‑ATTGTCGCTAAC‑TTT‑ACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTaaa                                                                                                                                                        <  1:871887/239‑1 (MQ=255)
                                                                                   tATTTTCGCTTTGATTCACGAACTGTTAATTGC‑ATACTCACGGTGGAAAAAAATAAAAAACCACATTTTTAGTGGTAGTTTGTGGG‑GGTGAATTTTTCCAAACAAATACAAAAA‑GTGG‑A‑GTTG‑CC‑TTG‑AG‑GAGCAAAAGTAAAAAGCAACGGGCATTA‑AAAAAGTGAACAGAG‑TTTTTCTCTAAT‑TTT‑ACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTaaaa                                                                                                                                                       <  1:992903/239‑1 (MQ=255)
                                                                                   tATTTTCGCGTTGATTCACGAACTGTTAATTAC‑ATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGC‑GGTGGAATTTTTCAGACAAAAAAAAAAA‑ATAG‑A‑GGTG‑TC‑AAT‑AT‑GTGCGGAAGTAATAAAAAACACGCAGTG‑AATAAAGTTACAGAG‑ATTGTGGCTAAC‑ATT‑ACCGCCCATATTTTTATCCCCATACCTGATAAGAGGGGGGGTAAACAaaaa                                                                                                                                                       >  1:883698/1‑239 (MQ=255)
                                                                                   tATTTTCGCGTTGATTCACGAACTGTTAATTAC‑ATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGC‑GGGGAAATTTTTCAAACAAAAAAAAAAA‑AAGG‑A‑GTTT‑GC‑ATT‑GT‑TGGCGGAAAGAATAAACAAAACGCAATGAAAAAAATTGA‑AGAG‑AATGTTGGTAAA‑CTT‑ACCCCCCTAGTTTTTTCCCGAATTCCCGTTCAAGAGGGGGAGAAACAaaaa                                                                                                                                                       >  2:1293926/1‑239 (MQ=255)
                                                                                     ttttCGCGTTGATTCACGAACTGTTAATTAC‑ATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGC‑GGTGAAATTTTCCAAACAAATAAAAAAA‑ATGG‑A‑GGTG‑CC‑CTG‑AT‑GAGCGCAAAGAAAAAGCAAAAAGCAGTG‑AATAAGATGAGAGAG‑ATTGTCGGTAAC‑TTT‑TCCGCCCAGAATTCTTCCCCAATGACTCAAGAAGAGGGGGGtaaaataaaaaa                                                                                                                                                     >  1:1028344/1‑237 (MQ=255)
                                                                                            gTTGATTCACGAACTGTTAATTAC‑ATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGC‑GGTGAATTTTTTTCCACAAAAAAAAAAA‑ATTG‑A‑GTGT‑CC‑ACT‑GT‑TAGCGGAAATAATAAGCAAAACGAAGCG‑AATAAAATGAGAGAG‑AATTTTGTTAAA‑CTT‑TCCGCCAAGATTTCTACCCCGAAACCTGAGGACGTGGGGGGTAAAATAAAACAGCTaaaa                                                                                                                                              >  1:86550/1‑238 (MQ=255)
                                                                                                          tGTTAATTAC‑ATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGC‑GGTGAATTTTTTCAGACAAAAAAAAAAA‑ATGG‑A‑GGTG‑CC‑ATG‑AT‑TAGGGAAAGGAATAAGCAAAAAGCAGGG‑AATTAATTGACAGAG‑ATTGTTTCTAAC‑TTT‑TCCGCCCTGATTTTTACCCCCATGCCTGATGAAGTGGGGGGTAAACTTAAAAAGCTAAAGGATGCCGGAAcac                                                                                                                                 >  1:1194302/1‑236 (MQ=255)
                                                                                                            ttAATTAC‑ATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGGGGAATTTTTCAAAACAAAAAAAAAAA‑TGGG‑G‑GTTG‑‑C‑ATG‑ATGGGGGGAAGGAAAAAAAAAAAAGGCAG‑G‑AATAAATTGAAAGAGAATTTTGGTTAAT‑TTT‑ACCACAATATTTTTTACTGAAATG‑CTGATAGAGTGGGGGGTAAAACAAAAAAGATAAAGGATTCCGaaaaagag                                                                                                                               >  2:273912/1‑233 (MQ=255)
                                                                                                            ttAATTAC‑ATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGC‑GGTGAATTTTTCCAGACAAATACAAAAA‑CTGG‑A‑GTTG‑CC‑ATG‑AT‑GAGCGAAAGTAATAAGCAACAGGCAGTG‑AATAAGTTGACAGAG‑ATTGTCGCTAAC‑TTT‑ACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTaaaa                                                                                                                                                       <  1:303499/214‑1 (MQ=255)
                                                                                                               aTTAC‑ATATCCACGGTGGAAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGG‑GGTGAATTTTTCAAGAAAAATAAAAAAA‑CGGG‑A‑GTTG‑CC‑ATG‑AG‑GGGCGAAAGTAATAAGCAACGGGAAGTG‑AATAAGTTGAAAGAG‑ATTGTCGCTAAC‑TTT‑ACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGa                                                                                                                           <  1:674231/239‑1 (MQ=255)
                                                                                                                     atatTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGC‑GGTGAATTTTTTCAGACAAATACAAAAA‑CTTG‑G‑GTTT‑CC‑ATG‑AT‑GAGCGAAAGAAATAAAAAAAAGGCATTG‑AGTTAAATGACAGCA‑ATTATCGCTTAC‑TTT‑ACCCCCATGATTTTTACCCGAATGCCCTCTGACGTGGTGGGTAAAATAAAAAAGATAAAGGATGCCGAAAAGTAGTCGATgggg                                                                                                                      >  1:707493/1‑239 (MQ=255)
                                                                                                                     atatTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGC‑GGTGAAATTTTTCAGACCAAAACAAAAA‑CTGG‑A‑GTTG‑CC‑ATG‑AT‑GAGGGAAAAGAATAAACAAAAGGCAGTG‑AAAAAGTTGACAGAG‑ATTGTCGCTAAC‑TTT‑ATCGCCACGCTTTCTACCCCAAAGCCTGATGACGTGGGGGGTAAAATAAAAAAGCTAAAAGATGCCCAAACATCGTCCGTgggg                                                                                                                      >  2:774665/1‑239 (MQ=255)
                                                                                                                      tatTCACGTGTGCAAAAAATATAAAACCTATTTTTTAGTGGGAGTTTGTGGC‑GTTGAATTTTCCCAGACAAAAACAAAAA‑CTGG‑A‑GTTG‑CC‑ATG‑AT‑GAGCGAAAGTAATAAGCAACGGGCAGTT‑AATAAGTTGACAGAG‑ATTGTCTCTACC‑TTT‑ACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGa                                                                                                                     <  1:480142/239‑1 (MQ=255)
                                                                                                                      tatTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGC‑GGTGAATTTTTCCAGACAAATACAAAAA‑CTGG‑A‑GTTG‑CC‑ATG‑AT‑GAGCGAAAGTAATAAGCAACAGGCAGTG‑AATAAGTTGACAGAG‑ATTGTCGTTAAC‑TTT‑ACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGa                                                                                                                     <  1:1166729/239‑1 (MQ=255)
                                                                                                                      tatTCACGGGGGAAAAAAATATAAAACCACTTTTTTAGTGGTAGTTTGTGGC‑GGTGAATTTTTCCAGACAAATACAAAAA‑CGGG‑A‑GTTG‑CC‑ATG‑AT‑GAGCGAAAGAAATAAGCAACAGGCAGTG‑AATAAGTTGACAGAG‑ATTGTCGCTAAC‑TTT‑ACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGa                                                                                                                     <  2:772923/239‑1 (MQ=255)
                                                                                                                       atTGACGGTGGCAAAAAATACAAAACCACATTTTTAGTGGTAGTTTGGGGC‑GGTGAATTTTTCCAGACAAATAAAAAAG‑CTGG‑A‑GTTG‑CT‑AGG‑AT‑GAGCGAAAGTAATAAGCAAGAGGCAGTG‑AATAAGTTGACAGAG‑ATTGTCGCTAAC‑TTT‑ACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGaa                                                                                                                    <  2:86550/239‑1 (MQ=255)
                                                                                                                       atTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGC‑GGTGAATTTTTTCAGACAAAAACAAAAA‑CTGG‑A‑GGTG‑CC‑ATG‑AT‑GAGCGGAAGTAAAAAACAAAAAGCAGGG‑AATAAATTGACAGAG‑ATTGTCGCTAAC‑TTT‑ACCGCCCTGATTTCTACCCGAATGCCCGATGACGTGGGGGGTAAACTAAAAAAACTAAAAGATGGCGAAACGTCGTCGGTggggga                                                                                                                    >  2:896696/1‑237 (MQ=255)
                                                                                                                       atTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGC‑GGTGAATTTTTTCAAACAAATACAAAAA‑ATGG‑A‑GTTG‑CC‑ATG‑AT‑GAGCGAAAATTAAAAGCAAAAAGCAGTG‑AATAAGATGAGAGAG‑ATTGTCGCTAAC‑ATT‑TACGCCATGCTTTTTAACCGGATACCTGATGAAGGGGGGGGTAAAAAAAAACAGCTAAAGGAGGCCGAAACGGCGTCGCTggggga                                                                                                                    >  1:48406/1‑237 (MQ=255)
                                                                                                                        tgcagggTGGAAAAAAATATAAAACCACTTGGTTGGGGGTAGTTGGTGGC‑GGTGAATTTTTCAAGAAAAAAAAAAAAA‑CGGG‑A‑GTGG‑CC‑ATG‑AT‑GAGCGAAAGTAATAAGCAAGAGGAAGGA‑AGTAGGTTGAAGGAG‑ATTGTCGATAAT‑TTT‑ACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGa                                                                                                                     <  1:127005/232‑1 (MQ=255)
                                                                                                                         tCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGC‑GGGGAAATTTTTCAAACAAAAAAAAAAA‑AAGG‑AGGTTG‑CC‑‑TG‑AT‑GAGGGGAAAAAATAAACAACAAGAAAGG‑AATAAGATGATCGAG‑ATTGTCGCCAAC‑ATT‑TCCGCCCCAATTTTTACCCGAAAGGCTGATGACGTTGGGGGGAAAATCAAACCGCTAAAAGAAGCCGAAAAATCGGCGGTTGGGaaaa                                                                                                                  >  2:191838/1‑239 (MQ=255)
                                                                                                                           tgggTGGAAAAAAATATAAAACCACATGTTTAGTGGGAGTTTGTGGG‑GGTGAATTTTTCAAGACAAATAAAAAAA‑CTGG‑A‑GTTG‑CC‑ATG‑GT‑GAGAGAAAGTAATAAGCAACAGGCAGTG‑AATAATTTGACAGAA‑ATTGGCGCTAAC‑TTT‑ACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAAtt                                                                                                                <  1:301350/237‑1 (MQ=255)
                                                                                                                             gggggCAAAAAATATAAAACCACATGTTTAGTGGTAGTTTGTGGC‑GATGATTTTTTCCAGAAAAACACAAAAG‑CTGG‑G‑TTTG‑CG‑ATG‑AT‑GGGCCAAAGTAATAAGCAACAGGCAGTG‑AATAAGTGGACAGAG‑ATTGGCGCTAAC‑TTG‑ACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTAt                                                                                                              <  1:779950/236‑1 (MQ=255)
                                                                                                                              gggacAAAAAATAAAAAACCCTCTTTTTAGTGGTATTTTGTGGG‑GGTGAAATTTTACAGAAAAATCAAAAAG‑CGTG‑A‑TGTG‑CA‑ATG‑AT‑GGGCAAAAATAATAAACATTAGGCAGTT‑AAGAAGTTGACGGAT‑ATTGTCTTTAAC‑TCT‑ACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTAt                                                                                                              <  2:1046192/234‑1 (MQ=255)
                                                                                                                              ggagaaaaaaaTATAAAACCAAATTTTTAGTGTTATTTTGGGGC‑GGTAAATTTTTCAAGACAAATAAAAAAA‑CTGG‑A‑GTGG‑CC‑ATG‑AG‑GAGCAAAAGTAAAAACCAAGAGGAATTA‑AATAAGTTGAAGGAG‑ATTGCCG‑TAACTTTT‑ACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTAt                                                                                                              <  2:1028344/233‑1 (MQ=255)
                                                                                                                               tGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGC‑GGTGAAATTTTTCCCAGAAAAATAAAAA‑ATTG‑A‑GGTG‑CA‑ACG‑AT‑AAGGGAAAGAAATAATTCACAGGCAGGG‑ACTAAGTTGTTAGAG‑AATGTTGCTAAC‑CTT‑ACCGACATTGTTTATACCCGAAAGCCTTATTACGTGGTGGAAAAACTAAAAAACCTAAACGAAGTCCAAACGTGGTCGGTGGGGAAAATTATc                                                                                                             >  1:1298972/1‑238 (MQ=255)
                                                                                                                                  cAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGC‑GGTGAAATTTTTCAGAAAAATAAAAAAA‑ATGG‑A‑GGTG‑TC‑ATG‑AT‑GAGCGAAAGTAAAAAAAAAAAAGCAGGG‑AATAAGATGACAGAG‑ATTGGCGCTAAA‑TTT‑ACCGCCATGATTTTTACCCGAATGGCTGATGACGTGGGGGGTAAACTAAAACAGCTAAAAGGAGCCCAAAAGTCGTCGATGGGGGAAAATATCTAcc                                                                                                         >  1:949350/1‑239 (MQ=255)
                                                                                                                                       aaaataAACCCAATTTTTTAGGGTTAGTTTGGGGC‑GGTGAATTTATCAAGAAAAAAAAAAAAA‑TTGG‑A‑GTGG‑CT‑ATG‑AT‑GAGCGAAAGTAATAAGCAACGGGCAGTA‑AATAAGACGAGAGGA‑TTTGCCGCTAAT‑TTT‑ACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACg                                                                                                    <  1:429396/236‑1 (MQ=255)
                                                                                                                                       aaTAAAAAACCACATTTTTAGTGGTAGTTTGGGGC‑GGTGAATTTTTCCAGAAAAATACAAAAA‑CTGG‑A‑GTTG‑CC‑AGG‑AT‑GAGCGAAAGTAATAAGCAACAGGCAGTA‑AATAAGTTAACAGAG‑ATTGTCGCTAAC‑TTT‑ACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACg                                                                                                    <  2:1194302/239‑1 (MQ=255)
                                                                                                                                                                gtttgggggC‑GGTGAATTTTTCCAGAAAAATACAAAAG‑CTGG‑A‑GTGG‑CC‑ATG‑AT‑GAGCAAAAGTAATAAGCAACAGGCAGTG‑AAAAAGTGGCAAAAG‑TTTGTCCTTAAC‑TTT‑ACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTTCCATACGATGTTCGACAACATGCAAAAAGCGa                                                                           <  1:88424/232‑1 (MQ=255)
                                                                                                                                                                atttggtgGC‑GGTGAATTTTTCCAAAAAAATACAAAAC‑CTGG‑A‑GTAT‑CC‑ATG‑AT‑GGACGAAATAAAAAACAAACAGGCATTG‑AATAAGTTATCATAT‑ATTTTCGCTAAT‑TTT‑ACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGa                                                                           <  2:707493/234‑1 (MQ=255)
                                                                                                                                                                aGTTTGTGGG‑GGTAAATTTTCCAAAAAAAATACAAAAA‑CTGG‑A‑GTTG‑‑C‑ATGAAG‑GAGCAAAATTAAAAAGCAACAGGCAGTA‑AATAAGTGAACAGAG‑ATTGTCGCTAAC‑TTG‑ACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGa                                                                           <  2:1019870/239‑1 (MQ=255)
                                                                                                                                                                aGTTTGTGGC‑GGTGAATTTTTCCAGACCAATACAAAAA‑CTGG‑A‑GGTG‑CC‑ATG‑AT‑GAGCGAAAGTAATAAACAAAAGGCAGTG‑AATAAGATGACAGAG‑ATTGTTGCTAAC‑TTT‑ACCCCCATGCTTTCTACCCGAAAGCCTGATGACGTGGTGGATAAACTAAAAAAGCTAAAGGATGCCGAAAAGTCGTCGGTGGGGAAAAATATCTACCATAAAATGTTTGACAACATACAAAAAACGa                                                                           >  1:1000310/1‑239 (MQ=255)
                                                                                                                                                                aGTTTGTGGC‑GGTGAATTTTTCCAGACAAATACAAAAA‑CTGG‑A‑TTTG‑CA‑AGG‑AG‑GAGCGAAAGTAAGAAGCAACAGGCGGTG‑AATAAGTTGACAGAG‑ATTGTCGCTAAC‑TTT‑ACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGa                                                                           <  2:117489/239‑1 (MQ=255)
                                                                                                                                                                      tgGC‑GGTAAATTTTCCCAGACAAATAAAAAAA‑CTGG‑A‑GTTG‑CC‑ATG‑AT‑AAGCGAAAGAAAAAACCAACAGGCAGTG‑AATAAGTTGACAGAG‑ATTGTCGCTAAC‑TTT‑ACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGAc                                                                      <  1:272301/238‑1 (MQ=255)
                                                                                                                                                                           ggTAAATTTTTCCAAAAAAATAAAAAAA‑CGGG‑A‑GTTG‑CA‑ATG‑AT‑GAGCAAAAGTAATAAGCAACAGGCAGTA‑AATAAGTTGACAGAG‑ATTGTCGCTAAC‑TTT‑ACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGa                                                                  <  1:531321/238‑1 (MQ=255)
                                                                                                                                                                                   tttCAAGAAAAAAAAAAAAA‑CTGG‑G‑GTTG‑CC‑ATG‑AT‑GAGCAAAAGAAATAAGCAACAGGAAGTG‑AATAAGTTGACAGGA‑ATTGCCGCTAAC‑TTT‑ACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTcctg                                                         <  2:945321/239‑1 (MQ=255)
                                                                                                                                                                                    tttcaaaaaaaaACAAAAAGCTGG‑A‑GTGC‑GA‑ATG‑AT‑GAGAGAAAGGAATAAAAAACAGGCGGTG‑AAAAAGTGGACAGAG‑TGTGTCGTTACC‑TTT‑CCCGCCAGGATTTCGACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTcctg                                                         <  2:713947/231‑1 (MQ=255)
                                                                                                                                                                                     acagaaCAAATACAAAAA‑CTGG‑‑‑GTTG‑CCAATA‑AG‑GAGCGAAAGAAATAAGCAACAGGCAGAA‑AATAAGTTAAAAGAG‑ATTGTCGC‑AAC‑TTTAACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTcctgcc                                                       <  2:251238/234‑1 (MQ=255)
                                                                                                                                                                                          aaaaaTAAAAAAA‑CTAG‑‑‑GTTGCCC‑ATG‑AT‑GAGAAAAATGAATAAGCAACAGGCAGTG‑AATAAGTTGACAGAG‑ATTGTCGTTAAC‑TTT‑ACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCa                                                  <  2:527294/237‑1 (MQ=255)
                                                                                                                                                                                           aaaaaACAAAAA‑TGGG‑A‑GTTG‑CC‑ATG‑AA‑GAGGAAAAGGAAAAAGCAACGGGAAGTA‑AAAAAGTGAACAGAG‑ATTGTCGCTAAT‑TTT‑ACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTc                                                   <  2:59070/236‑1 (MQ=255)
                                                                                                                                                                                            aaaTACAAAAA‑CGGG‑A‑GTTG‑CC‑ATA‑AT‑GAGGAAAAGTAATAAGAAACAGGCAGTA‑AATAAGTTGAAAGAG‑ATTGTCGCTAAC‑TTT‑ACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAgg                                                <  1:1293926/239‑1 (MQ=255)
                                                                                                                                                                                             aaTAAAAAAC‑CTGG‑A‑GTGG‑CC‑ATG‑AG‑GAGCGAAAGAAATAAGAAACAGGCAGTG‑AAAAATTTGAAAGAG‑ATTGTCGCTAAC‑TTT‑ACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGa                                               <  2:949350/239‑1 (MQ=255)
                                                                                                                                                                                                   aaaa‑CTGG‑A‑GTTG‑CC‑AGG‑AT‑GAGCAAAAATAATAAGCAAGAGGCAGTG‑AATAAGTTGACAGAG‑ATTGTCGCTACT‑TTT‑ACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCgg                                         <  2:507303/239‑1 (MQ=255)
                                                                                                                                                                                                   aaaa‑CGGG‑A‑GTTT‑CC‑AGG‑AG‑GAGCAAAATTAATAAGCAACAGGCAGTA‑AAAAAGTTGACAGAG‑ATTGTCGCTAAC‑TTA‑ACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCgg                                         <  1:509066/239‑1 (MQ=255)
                                                                                                                                                                                                     aa‑CTGG‑A‑TTGG‑CC‑ATG‑AT‑GAGCAAAAGTAAAAAGCAAAAGGAAGTG‑AATAAGTTGAAAGGT‑ATGGCCGCTAAT‑TTT‑ACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCgggg                                       <  1:981200/239‑1 (MQ=255)
                                                                                                                                                                                                     aa‑CTGG‑A‑GTTG‑CC‑ATG‑AT‑GAGCGAAAGTAATAAGCAACAGGCAGTA‑AATAAGTTGACAGAG‑ATTGTCGCTACC‑TTT‑ACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCgggg                                       <  2:792659/239‑1 (MQ=255)
                                                                                                                                                                                                     aa‑CGGG‑T‑GTGG‑CC‑ATG‑AT‑GAGGAAAAATAATAAACAACAGGCAGTG‑AATAAGTTGAAGGAG‑ATTGCCGTAAAC‑TTT‑ACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCgggg                                       <  2:395858/239‑1 (MQ=255)
                                                                                                                                                                                                      a‑CTGG‑A‑GTTG‑CC‑ATG‑AT‑GAGCGAAAGTAATAAGCAACAGGCAGTG‑AATAAGTTGACAGAG‑ATTGTCGCTAAC‑TTT‑ACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACGCCGGGga                                      >  1:798271/1‑239 (MQ=255)
                                                                                                                                                                                                           g‑A‑GTTG‑CC‑ATG‑AT‑GAGCGAAAGTAATAAGCAACAGGCAGTG‑AATAAGTTGACAGAG‑ATTGTCGCTAAC‑TTT‑ACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGAtt                                  >  2:204027/1‑239 (MQ=255)
                                                                                                                                                                                                                  g‑CC‑ATG‑AT‑GAGCGAAAGTAATAAGCAACAGGCAGTG‑AATAAGTTGACAGAG‑ATTGTCGCTAAC‑TTT‑ACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGt                              >  1:1009050/1‑238 (MQ=255)
                                                                                                                                                                                                                  g‑CC‑ATG‑AT‑GAGCGAAAGTAATAAGCAACAGGCAGTG‑AATAAGTTGACAGAG‑ATTGTCGCTAAC‑TTT‑ACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGt                              >  1:1270539/1‑238 (MQ=255)
                                                                                                                                                                                                                     c‑ATG‑AT‑GAGGAAAAGTAATAAGAAACTGTCAGTG‑AATAAGTTGACAGAG‑ATGGTCGTTAAC‑TTT‑ACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGttc                            <  1:176245/238‑1 (MQ=255)
                                                                                                                                                                                                                     c‑ATG‑AT‑GAGCGAAAGTAAAAAGCAACAGGCAGTG‑AATAAGTTGACAGAG‑ATTGCCGCTAAC‑TTT‑ACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGttc                            <  1:896696/238‑1 (MQ=255)
                                                                                                                                                                                                                      aatg‑ag‑gAGCAAAGATAAAAAGCAACGGGCAGGG‑AATAAGTTGCAAGAG‑ATTGCGGCAAAT‑TTA‑ACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTGCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGttc                            <  2:740454/237‑1 (MQ=255)
                                                                                                                                                                                                                       aagggt‑gaGCAAAAGAAATAAGAAACAGGCAGTG‑AAGAAGTTGAAAGAG‑ATTGTCGCTAAC‑TTT‑ACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGttc                            <  2:740417/233‑1 (MQ=255)
                                                                                                                                                                                                                           at‑gaGCGAAAGTAATAAGCAACAGGCAGTG‑AATAAGTTGACAGAG‑ATTGTCGCTAAC‑TTT‑ACCGCCATGATTTCTACCCGAATGCCTGATGACgtg                                                                                                                                                                      >  2:73660/1‑96 (MQ=255)
                                                                                                                                                                                                                           at‑gaGCAAAAGTAATAAGCAACAGGCAGTG‑AATAAGTTGACAGAG‑ATTGTCGCTAAC‑TTT‑ACCGCCATGATTTCTACCCGAATGCCTGATGACgtg                                                                                                                                                                      <  1:73660/96‑1 (MQ=255)
                                                                                                                                                                                                                              gggggaaagaaaAAAACACAAGGCATTA‑AATATGTTGAAGGAG‑CTGGTCTATAAT‑TTT‑CCCGCCATGATTTCTACCCGAATGCCTGATGACGTTGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTCCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGtt                      <  2:1260604/234‑1 (MQ=255)
                                                                                                                                                                                                                                          taaGCAACAGGCAGTG‑AATAAGTTGACAGAG‑ATTGTCGCTAAC‑TTT‑ACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTccc         >  1:1133048/1‑239 (MQ=255)
                                                                                                                                                                                                                                             gCAACAGAGAATG‑AAAAAGGTGACAGAG‑TGTGTCACTACC‑TTT‑ACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTGCGTTAAAGTCGGTTCCCg        <  1:811517/237‑1 (MQ=255)
                                                                                                                                                                                                                                                 cAGGCAGTG‑AATAAGTTGACAGAG‑ATTGTCGCTAAC‑TTT‑ACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCTTccc  >  2:913483/1‑239 (MQ=255)
                                                                                                                                                                                                                                                 cAGGCAGTG‑AATAAGTTGACAGAG‑ATTGTCGCTAAC‑TTT‑ACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCTTcc   >  2:663346/1‑238 (MQ=255)
                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                  
ATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTAC‑ATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGC‑GGTGAATTTTTCCAGACAAATACAAAAA‑CTGG‑A‑GTTG‑CC‑ATG‑AT‑GAGCGAAAGTAATAAGCAACAGGCAGTG‑AATAAGTTGACAGAG‑ATTGTCGCTAAC‑TTT‑ACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCTTCCC  >  NC_000913/3206255‑3206725

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑