BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	4,530	T→G	11.3%	V266G (GTG→GGG)	thrC →	L‑threonine synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,530	0	T	G	11.3%	396.4 / 2.5	146	V266G (GTG→GGG)	thrC	L‑threonine synthase
Reads supporting (aligned to +/- strand): ref base T (16/104); new base G (23/2); total (40/106)
Fisher's exact test for biased strand distribution p-value = 2.74e-14
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 149 total aligned reads displayed.

TCAGGCGCTGGTGAAGCAGGCGTTTGATGATGAAGAACTGAAAGTGGCGCTAGGGTTAAACTCGGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCTGTTGCGCAGCTGCCGCAGGAGACGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCA‑CTCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAACGTGAACGATACCGTGCCACGTTTCCTGCACGACGGTCAGTGGTCACCCAAAGCGACTCAGGCGACGTTATCCAACGCGATGGACGTGAGT‑CAGCCGAACAACTGGCCGCGTGTGGAAGAGTTGTTCCGCCG‑CAAAATCTGGCAACTGAAAGAGCTGGGTTATGCAGCCGTGGATGATGAAACCACGCAACAGACAATGCGTGAGTTAAAAGA  >  NC_000913/4300‑4767

         ggTGAAGCAGGCGTTTGATGATGAAGAACTGAAAGTGGCGCTAGGGTTAAACTCGGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCTGTTGCGCAGCTGCCGCAGGAGACGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAGAGGA‑CCCGGGCTGCCGGGGGAACGTTTTAttgttg                                                                                                                                                                                                                                >  2:1421263/1‑239 (MQ=255)

         ggTGAAGCAGGCGTTTGATGATGAAGAACTGAAAGTGGCGCTAGGGTTAAACTCGGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCTGTTGCGCAGCTGCCGCAGGAGACGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGGCTGCTGGCGCAATCA‑CTCGGTCTGCCGGGGAAACGTTTTAtttgt                                                                                                                                                                                                                                 >  1:237218/1‑235 (MQ=255)

          gTGAAGCAGGCGTTTGATGATGAAGAACTGAAAGTGGCGCTAGGGTTAAACTCGGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCTGTTGCGCAGCTGCCGCAGGAGACGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCA‑CTCGGTCTGCCCGGGAGACGTTTTAttgttg                                                                                                                                                                                                                                >  2:1003762/1‑238 (MQ=255)

          gTGAAGCAGGCGTTTGATGATGAAGAACTGAAAGTGGCGCTAGGGTTAAACTCGGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCTGTTGCGCAGCTGCCGCAGGAGACGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGGCGTCTGGGGGAGCGA‑CCAGGGCTGCCGGGGAGACGGttttttttgga                                                                                                                                                                                                                               >  2:913578/1‑234 (MQ=255)

              aGCAGGCGTTTGATGATGAAGAACTGAAAGTGGCGCTAGGGTTAAACTCGGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCTGTTGCGCAGCTGCCGCAGGAGACGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCA‑CTCGGTCTGCCGGTGAAACGTTTTATTGCTGCGAcc                                                                                                                                                                                                                           <  1:1228452/239‑1 (MQ=255)

               gCAGGCGTTTGATGATGAAGAACTGAAAGTGGCGCTAGGGTTAAACTCGGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCTGTTGCGCAGCTGCCGCAGGAGACGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGGCTGCTGGCGGAGGCA‑CTCGGGCCTGCGGGGGAACGGTTTTTTTCTGCGGCCa                                                                                                                                                                                                                          >  2:124942/1‑239 (MQ=255)

                    cGTTTGATGATGAAGAACTGAAAGTGGCGCTAGGGTTAAACTCGGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCTGTTGCGCAGCTGCCGCAGGAGACGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCA‑CTCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAAc                                                                                                                                                                                                                        <  1:672538/236‑1 (MQ=255)

                    cGTTTGATGATGAAGAACTGAAAGTGGCGCTAGGGTTAAACTCGGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCTGTTGCGCAGCTGCCGCAGGAGACGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCA‑CTCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAAc                                                                                                                                                                                                                        <  1:1100182/236‑1 (MQ=255)

                    cGTTTGATGATGAAGAACTGAAAGTGGCGCTAGGGTTAAACTCGGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCTGTTGCGCAGCTGCCGCAGGAGACGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCA‑CTCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAAc                                                                                                                                                                                                                        <  2:281742/236‑1 (MQ=255)

                    cGTTTGATGATGAAGAACTGAAAGTGGCGCTAGGGTTAAACTCGGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCTGTTGCGCAGCTGCCGCAGGAGACGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCA‑CTCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAAc                                                                                                                                                                                                                        <  1:587455/236‑1 (MQ=255)

                    cGTTTGATGATGAAGAACTGAAAGTGGCGCTAGGGTTAAACTCGGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCTGTTGCGCAGCTGCCGCAGGAGACGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCA‑CTCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAAc                                                                                                                                                                                                                        <  1:764167/236‑1 (MQ=255)

                    cGTTTGATGATGAAGAACTGAAAGTGGCGCTAGGGTTAAACTCGGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCTGTTGCGCAGCTGCCGCAGGAGACGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCA‑CTCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAAc                                                                                                                                                                                                                        <  1:743889/236‑1 (MQ=255)

                    cGTTTGATGATGAAGAACTGAAAGTGGCGCTAGGGTTAAACTCGGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCTGTTGCGCAGCTGCCGCAGGAGACGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCA‑CTCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAAc                                                                                                                                                                                                                        <  1:1522326/236‑1 (MQ=255)

                    cGTTTGATGATGAAGAACTGAAAGTGGCGCTAGGGTTAAACTCGGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCTGTTGCGCAGCTGCCGCAGGAGACGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGCCTGCTGGCGAAGTCA‑CTCGGTCCGCCGGGGGAACGTTTTATTGCTGCGACCAACgcg                                                                                                                                                                                                                     >  1:951894/1‑237 (MQ=255)

                        tgatgatgaAGAGCTGAAAGTGGCGCTAGGGTTAAACTCGGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCTGTTGCGCAGCTGCCGCAGGAGACGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCA‑CTCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAACGTGAACg                                                                                                                                                                                                                 <  2:1025045/239‑1 (MQ=255)

                        tgatgatgaAGAACTGAAAGTGGCGCTAGGGTTAAACTCGGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCTGTTGCGCAGCTGCCGCAGGAGACGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCA‑CTCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAACGTGAACg                                                                                                                                                                                                                 <  1:1377571/239‑1 (MQ=255)

                              tgaAGAACTGAAAGTGGCGCTAGGGTTAAACTCGGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCTGTTGCGCAGCTGCCGCAGGAGACGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCCTGCGACCGCA‑CTCGGTCTGCCGGGGAAAAGATTTATTGTTGCGCCCCACGTGAACGaacacc                                                                                                                                                                                                           >  2:206604/1‑234 (MQ=255)

                              tgaAGAACTGAAAGTGGCGCTAGGGTTAAACCCGGCTACCTCGACTACCAACAGCTGTTTGCTGCAGCAGATTTGCTACTACTTTGAAGATGTCGCGCAGCTGCCGCAGGAGACGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGTGAAGTCA‑CTCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCa                                                                                                                                                                                                                          <  2:1426505/224‑1 (MQ=255)

                               gaagaaCTGAAAGTGGCGCTAGGGTTAAACTCGGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCTGTTGCGCAGCTGCCGCAGGAGACGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCA‑CTCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAACGTGAACGATACCGt                                                                                                                                                                                                          <  1:124942/239‑1 (MQ=255)

                                 agaaCTGAAAGTGGCGCTAGGGTTAAACTCGGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCTGTTGCGCAGCTGCCGCAGGAGACGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCA‑CTCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAACGTGAACGATACCGTGc                                                                                                                                                                                                        <  1:861410/239‑1 (MQ=255)

                                 agaaCTGAAAGTGGCGCTAGGGTTAAACTCGGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCTGTTGCGCAGCTGCCGCAGGAGACGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCA‑CTCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAACGTGAACGATACCGTGc                                                                                                                                                                                                        <  1:710175/239‑1 (MQ=255)

                                        aaaGTGGCGCTAGGGTTAAACTCGGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCTGTTGCGCAGCTGCCGCAGGAGACGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGGCCGCTTGCGCAGTCC‑CTCGGTCTGGCGAGGAAACGTTTTATTTCTTGGCCCCACCTGAACGa                                                                                                                                                                                                                >  1:1426505/1‑224 (MQ=255)

                                             ggCGCTAGGGTTAAACTCGGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCTGTTGCGCAGCTGCCGCAGGAGACGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGGAGTCA‑CTCGGCCTGCCGGTGAAAAGTTTTTTTGCTGCGCGCAACGGGAACGATACCGGGCCACGTTTCCtt                                                                                                                                                                                             >  2:574032/1‑237 (MQ=255)

                                             ggCGCTAGGGTTAAACTCGGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCTGTTGCGCAGCTGCCGCAGGAGACGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCA‑CTCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAACGTGAACGATACCGTGCCACGTTTCCt                                                                                                                                                                                              <  2:1165161/237‑1 (MQ=255)

                                               cgcTAGGGTTAAACTCGGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCTGTTGCGCAGCTGCCGCAGGAGACGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGGCTGCTGGCGAAGTCA‑CTCGGGCTGCCGGTGAAACGGTTTATTTCTGCGACCAACGTGAACGATACCGTGGCACCGTTTCTGCac                                                                                                                                                                                          >  1:927205/1‑239 (MQ=255)

                                                gttAGGTTTAAACTCGGCTAACTCGATTAACATCAGCCGTTTGCTTGCGCAGATTTGCTACTACTTTGAAGCTGTTGCGCAGCTGCCGCAGGAGACGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCA‑CTCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAACGTGAACGATACCGTGCCACGTTTCCTGCacg                                                                                                                                                                                         <  2:932390/237‑1 (MQ=255)

                                                gcTAGGGTTAAACTCGGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCTGTTGCGCAGCTGCCGCAGGAGACGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCA‑CTCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAACGTGAACGATACCGTGCCACGTTTCCTGCacg                                                                                                                                                                                         <  1:153346/239‑1 (MQ=255)

                                                gcTAGGGTTAAACTCGGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCTGTTGCGCAGCTGCCGCAGGAGACGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCA‑CTCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAACGTGAACGATACCGTGCCACGTTTCCTGCacg                                                                                                                                                                                         <  2:146863/239‑1 (MQ=255)

                                                  tAGGGTTAAACTCGGCTAACTCGATTACCATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCTGTTGCGCAGCTGCCGCAGGAGACGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCA‑CTCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAACGTGAATGATACCGTGCCACGTTTCCTGCacga                                                                                                                                                                                        <  1:910058/238‑1 (MQ=255)

                                                       ttAAACTCGGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCTGTTGCGCAGCTGCCGCAGGAGACGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCA‑CTCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAACGTGAACGATACCGTGCCACGTTTCCTGCACGACGGTc                                                                                                                                                                                   <  2:1221748/238‑1 (MQ=255)

                                                       ttAAACTCGGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCTGTTGCGCAGCTGCCGCAGGAGACGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCA‑CTCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAACGTGAACGATACCGTGCCACGTTTCCTGCACGACGGTc                                                                                                                                                                                   <  2:779949/238‑1 (MQ=255)

                                                          aaCTCGGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCTGTTGCGCAGCTGCCGCAGGAGACGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCA‑CTCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAACGTGAACGATACCGTGCCACGTTTCCTGCACGACGGTCAGTg                                                                                                                                                                               <  1:315381/239‑1 (MQ=255)

                                                          aaCTCGGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCTGTTGCGCAGCTGCCGCAGGAGACGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCA‑CTCGGTCTGCCGGGGAAAAGTTTTTTTTCTTCGACCCACCGGGACGATAACGTGCCCCGGTTCCTCCacgaccggccggg                                                                                                                                                                               >  1:821767/1‑231 (MQ=255)

                                                            cTCGGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCTGTTGCGCAGCTGCCGCAGGAGACGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCA‑CTCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAACGTGAACGATACCGTGCCACGTTTCCTGCACGACGGTCAGTGGt                                                                                                                                                                             <  1:1011034/239‑1 (MQ=255)

                                                               ggCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCTGTTGCGCAGCTGCCGCAGGAGACGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCA‑CTCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAACGTGAACGATACCGTGCCACGTTTCCTGCACGACGGTc                                                                                                                                                                                   <  2:747595/230‑1 (MQ=255)

                                                               ggCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCTGTTGCGCAGCTGCCGCAGGAGACGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCA‑CTCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAACGTGAACGATACCGTGCCACGTTTCCTGCACGACGGTc                                                                                                                                                                                   >  1:747595/1‑230 (MQ=255)

                                                                gCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCTGTTGCGCAGCTGCCGCAGGAGACGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTTCTGGCGAAGTCA‑CCCGGTCTTCCGGTGAAAAGTTTTTTTTCTGCGACCAACGTGAAAGATACCAGGCCCCCGTTCCTCCACCACGGTcgcgtggcaa                                                                                                                                                                          >  2:1347245/1‑229 (MQ=255)

                                                                         aTTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCTGTTGCGCAGCTGCCGCAGGAGACGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGGGTGCTGGCGGAGTCAGCCCGGCCGACCGGGGAAA‑GTTTTTTTTCTTGGACCGACCTGGACGATACCATCCCCCGGTTCCTTCTCGGCCGGCAGCTGTCACCCAAAGCgg                                                                                                                                                                 >  2:178708/1‑237 (MQ=255)

                                                                          ttAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCTGTTGCGCAGCTGCCGCAGGAGACGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCA‑CTCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAACGTGAACGATACCGTGCCACGTTTCCTGCACGACGGTCAGTGGTCACCCAAAGCGACt                                                                                                                                                               <  1:728520/239‑1 (MQ=255)

                                                                              cATCTGCCGTGTGCTGGCGCAGAGTTGCTACTACTTTGAAGCTGTTGCGCAGCTGCCGCAGGAGACGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCA‑CTCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAACGTGAACGATACCGTGCCACGTTTCCTGCACGACGGTCAGTGGTCACCCAAAGCGACTCAgg                                                                                                                                                           <  2:1079406/239‑1 (MQ=255)

                                                                                   gCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCTGTTGCGCAGCTGCCGCAGGAGACGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCA‑CTCGGTCTGCCGGTGAAACGTTTTTTTTCTGCGACCAACGTGAACGCTACCGGGCCACGTTTCCTGCACGACGGTCAGTGGGCACCCACAGCGACCCAGGCGCCg                                                                                                                                                      >  1:576906/1‑239 (MQ=255)

                                                                                       tttGCTGGCGCAGATTTGCTACTACTTTGAAGCTGTTGCGCAGCTGCCGCAGGAGACGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGGTTTCTGGGGGAGGCA‑CCCGGTCCGCCGGGGAAACGCTTTATTGCTGGGGCCAACGTGAACGATACCGTGCCCAGTTTCCCTCACGACGGGCCGTGGTCCCCCAAAGCGCTCCAGGCGACGTTAt                                                                                                                                                  >  2:748399/1‑239 (MQ=255)

                                                                                        ttGCTGGCGCAGATTTGCTACTACTTTGAAGCTGTTGCGCAGCTGCCGCAGGAGACGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCA‑CTCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAACGTGAACGATACCGTGCCACGTTTCCTGCACGACGGTCAGTGGTCACCCAAAGCGACTCAGGCGACGTTATc                                                                                                                                                 <  1:504153/239‑1 (MQ=255)

                                                                                         tGCTGGCGCAGATTTGCTACTACTTTGAAGCTGTTGCGCAGCTGCCGCAGGAGACGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCA‑CTCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAACGTGAACGATACCGTGCCACGTTTCCTGCACGACGGTCAGTGGTCACCCAAAGCGACTCAGGCGACGTTATcc                                                                                                                                                <  1:1386445/239‑1 (MQ=255)

                                                                                              gcgcAGATTTGCTACTACTTTGAAGCTGTTGCGCAGCTGCCGCAGGAGACGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTTCCGGCGAAGACA‑CCCGGGCTGTCGGGGGAACGTTTTATTTGTGCGACCAACGGGAACGAAACCGGGCCACGCTTCCTTCCCGGCCGGCCGAGGTCACCCCCAACGACCCAGGGGGCGCTATctccag                                                                                                                                            >  2:496802/1‑233 (MQ=255)

                                                                                                     tttGCTCCTCATTTGAAGCGGTTGCGCAGCTGCCGCAGGAGACGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCA‑CTCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAACGTGAACGATACCGTGCCACGTTTCCTGCACGACGGTCAGTGGTCACCCAAAGCGACTCAGGCGACGTTATCCAACGCGATGGa                                                                                                                                     <  2:967223/238‑1 (MQ=255)

                                                                                                        gCTACTACTTTGAAGCTGTTGCGCAGCTGCCGCAGGAGACGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCA‑CTCGGTCTGCCGGTGAAACGTTTTATTGCTGCGAcc                                                                                                                                                                                                                           <  1:523956/149‑1 (MQ=255)

                                                                                                           gctactTTGAAGCTCTTGCGCAGCTGCCGCAGGAGACGCGCAACCAGGTGGTTGTCGCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCA‑CTCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAACGAGAACGATACCGTGCCACGTTTCCTGCACGACGGTCAGTGGTCACCCAAAGCGACTCAGACGACGTTATCCAACGCGATGGACGTGAGt                                                                                                                              <  2:951894/238‑1 (MQ=255)

                                                                                                           actactTTGAAGCTGTTGCGCAGCTGCCGCAGGAGACGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCA‑CTCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAACGTGAACGATACCGTGCCACGTTTCCTGCACGACGGTCAGTGGTCACCCAAAGCGACTCAGGCGACGTTATCCAACGCGATGGACGTGAGt                                                                                                                              <  1:1209857/239‑1 (MQ=255)

                                                                                                            ctactTTGAAGCTGTTGCGCAGCTGCCGCAGGAGACGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCA‑CTCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAACGTGAACGATACCGTGCCACGTTTCCTGCACGACGGTCAGTGGTCACCCAAAGCGACTCAGGCGACGTTATCCAACGCGATGGACGTGAGT‑c                                                                                                                            <  1:59509/239‑1 (MQ=255)

                                                                                                             tactTTGAAGCTGTTGCGCAGCTGCCGCAGGAGACGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCA‑CTCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAACGTGAACGATACCGTGCCACGTTTCCTGCACGACGGTCAGTGGTCACCCAAAGCGACTCAGGCg                                                                                                                                                         <  1:1313167/210‑1 (MQ=255)

                                                                                                              actTTGAAGCTGTTGCGCAGCTGCCGCAGGAGACGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGGAGGCA‑CCCGGTCTGCCGGGGGAACGGTTTTTTTCTGCGACCCACGTGAAAGAAACCGTGCCCCGTTTCCTGCCCGCCCGTCAGGGGGTCCCCAAAACCACTCCAGCGACGTTTTCCAACCCGCTGGACGGGTGT‑Ca                                                                                                                           >  2:1251321/1‑238 (MQ=255)

                                                                                                              actTTGAAGCTGTTGCGCAGCTGCCGCAGGAGACGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCA‑CTCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAACGTGAACGATACCGTGCCACGTTTCCTGCACGACGGTCAGTGGTCACCCAAAGCGACTCAGGCGACGTTATCCAACGCGATGGACGTGAGT‑CAg                                                                                                                          <  1:1003762/239‑1 (MQ=255)

                                                                                                              acgtggAAGCTGTTGCGCAGCTGCCGCAGGAGACGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCA‑CTCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAACGTGAACGATACCGTGCCACGTTTCCTGCACGACGGTCAGTGGTCACCCAAAGCGACTCAGGCGACGTTATCCAACGCGATGGACGTGAGT‑CAg                                                                                                                          <  2:201533/234‑1 (MQ=255)

                                                                                                               ctTTGAAGCTGTTGCGCAGCTGCCGCAGGAGACGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGGCTTCTGGCGAAGCCA‑CTCGGTCTGCCGGGGAAAAGTTTTATTTTTTCGACCAACGTGGACGAAAACGTGCCACGTTTCCTGTACGACGGGCAGAGGTCACCCAAAACGGCTCAGGCGGCGTTTACCAACCCGAAgggagtggggacg                                                                                                                           >  2:275911/1‑227 (MQ=255)

                                                                                                                tttGAAGCTGTTGCGCAGCTGCCGCAGGAGACGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCA‑CTCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAACGTGAACGATACCGTGCCACGTTTCCTGCACGACGGTCAGTGGTCACCCAAAGCGACTCAgg                                                                                                                                                           <  1:1258841/205‑1 (MQ=255)

                                                                                                                 ttGAAGCTGTTGCGCAGCTGCCGCAGGAGACGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCA‑CTCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAACGTGAACGATACCGTGCCACGTTTCCTGCACGACGGTCAGTGGTCACCCAAAGCGACTCAGGCGACGTTATCCAACGCGATGGACGTGAGT‑CAGCCg                                                                                                                       <  1:362254/239‑1 (MQ=255)

                                                                                                                  tGAAGCTGTTGCGCAGCTGCCGCAGGAGACGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGACGTCA‑CTCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAACGTGAACGATACCGTGCCACGTTTCCTGCACGACGGTCAGTGGTCACCCAAAGCGACTCAGGCGACGTTATCCAACGCGATGGACGTGAGT‑CAGCCGa                                                                                                                      <  1:206604/239‑1 (MQ=255)

                                                                                                                   gAAGCTGTTGCGCAGCTGCCGCAGGAGACGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCA‑CTCGGTCTGCCGGGGAAAAGTTTTATTTCTGCGACCAACGTGAACGATACCGTGCCACGTTTCCTGCACGACGGTCAGTGGGCACCCAAAGCGACTCAGGCGACGTTATCCAACGCGATGGACGGGAGT‑CAGACGa                                                                                                                      >  1:787164/1‑238 (MQ=255)

                                                                                                                    aaGCTGTTGCGCAGCTGCCGCAGGAGACGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCA‑CTCGGTCTGCCGGTGaaa                                                                                                                                                                                                                                             <  1:427125/119‑1 (MQ=255)

                                                                                                                         gTTGCGCAGCTGCCGCAGGAGACGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCA‑CTCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAACGTGAACGATACCGTGCCACGTTTCCTGCACGACGGTCAGTGGTCACCCAAAGCGACTCAGGCGACGTTATCCAACGCGATGGACGTGAGT‑CAGCCGAACAACTg                                                                                                               >  1:740642/1‑239 (MQ=255)

                                                                                                                         gTTGCGCAGCTGCCGCAGGAGACGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGGCTGCTGGCGAAGGCC‑CCCGGTCTGCCGGGGAAACGTTTTATTTCTGCGACCAACGTGAACCATACCGTGCCCCGGTTCCTGCCCGACGGGCAGTGGGCACCCAAAGCGACCCAGGCGACGGTATCCCACGCGATGGACGGGAGG‑CAGCCGGACAACTg                                                                                                               >  2:922243/1‑239 (MQ=255)

                                                                                                                            gcgcAGCTGCCGCAGGAGACGCGCAACCAGCTTGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCA‑CTCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAACGTGAACGATACCGTGCCACGTTTCCTGCACGACGGTCAGTGGTCACCCAAAGCGACTCAGGCGACGTTATCCAACGCGATGGACGTGAGT‑CAGCCGAACAACTGGcc                                                                                                            <  1:173860/239‑1 (MQ=255)

                                                                                                                            gcgcAGCTGCCGCAGGAGACGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCA‑CTCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAACGTGAACGATACCGTGCCACGTTTCCTGCACGACGGTCAGTGGTCACCCAAAGCGACTCAGGCGACGTTATCCAACGCGATGGACGTGAGT‑CAGCCGAACAACTGGcc                                                                                                            <  1:1018215/239‑1 (MQ=255)

                                                                                                                              gcAGCTGCCGCAGGAGACGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCA‑CTCGGTCTGCCGGTGAAACGTTTTATTTCTGCGACCAACGTGAACGATACCGTGCCACGTTTCCTGCACGACGGTCAGTGGTCACCCAAAGCGACTCAGGCGACGTTATCCAACGCGATGGACGTGAGT‑CAGCCGAACAACTGGCcgc                                                                                                          <  1:275911/239‑1 (MQ=255)

                                                                                                                              gcAGCTGCCGCAGGAGACGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCA‑CTCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAACGTGAACGATACCGTGCCACGTTTCCTGCACGACGGTCAGTGGTCACCCAAAGCGACTCAGGCGACGTTATCCAACGCGATGGACGTGAGT‑CAGCCGAACAACTGGCcgc                                                                                                          <  2:206753/239‑1 (MQ=255)

                                                                                                                                  cTGCCGCAGGAGACGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGGCTGCTGGCGGAGGCA‑CTCCGTCTGGCGGGGGAACGTTTTTTTTGTGCGACCAACCGGGACGATACCCTGCCCCCGTTTCTTCACGGCGGTCAGTTGGCCCCCCAAGCGACTCAGGCGACGTTATCCCACACGATGGGCGTGGGG‑CAACAGCACAACAGGCCCCgggt                                                                                                      >  2:402648/1‑239 (MQ=255)

                                                                                                                                    gCCGCAGGAGACGGACAGCCAGGTGGTTGTCTCGGTGCCAAGCGGAAACGTCGGCGCGTTGACGGCGGGTCTGCTGGCGAAGTCA‑CTCTGTCTGCCGGTGAAACGGTTTATTGCTGCGACCAACGTGAACGATACCGTGCCACGTTTCCTGCACGACGGTCAGTGGTCACCCAAAGCGACTCAGGCGACGTTATCCAACGCGATGGACGTGAGT‑CAGCCGAACAACTGGCCGCGTGTgg                                                                                                    <  2:834504/239‑1 (MQ=255)

                                                                                                                                               cgcgCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCA‑CTCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAACGTGAACGATACCGTGCCACGTTTCCTGCACGACGGTCAGTGGTCACCCAAAGCGACTCAGGCGACGTTATCCAACGCGATGGACGTGAGT‑CAGCCGAACAACTGGCCGCGTGTGGAAGAGTTGTTc                                                                                         <  2:1299399/239‑1 (MQ=255)

                                                                                                                                                                          gaaGCGGAAACTTCGGCGAGTTGACGGCGGGTCTGCTGGCGATGTAA‑CTCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAACGTGAACGATACCGTGCCACTTTTCCTGCACGACGGTCAGTGGTCACCCAAAGCGACTCAGGCGACGTTATCCAACGCGATGGACGTGAGT‑CAGCCGAACAACTGGCCGCGTGTGGAAGAGTTGTTCCGCCG‑CAAAATCTGGCAACTGAAagag                                                             <  2:681493/238‑1 (MQ=255)

                                                                                                                                                                          cAAGCGGAACCTTCGGCGATTTGACGGCGGGTGTGCTGGCGAAGTCA‑CTCGGTCTGCCGGTGAAATGTTTTATTGCTGCGACCAACGTGAACGATACCGTGCCACGTTTCCTGCACGACGGTCAGTGGTCACCCAAAGCGACTCAGGCGACGTTATCCAACGCGATGGACGTGAGT‑CAGCCGAACAACTGGCCGCGTGTGGAAGAGTTGTTCCGCCG‑CAAAATCTGGCAACTGAAagag                                                             <  2:113142/239‑1 (MQ=255)

                                                                                                                                                                          cAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCA‑CTCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAACGTGAACGATACCGTGCCACGTTTCCTGCACGACGGTCAGTGGTCACCCAAAGCGACTCAGGCGACGTTATCCAACGCGATGGACGTGAGT‑CAGCCGAACAACTGGCCGCGTGTGGAAGAGTTGTTCCGCCG‑CAAAATCTGGCAACTGAAagag                                                             <  1:1347245/239‑1 (MQ=255)

                                                                                                                                                                          cAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCA‑CTCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAACGTGAACGATACCGTGCCACGTTTCCTGCACGACGGTCAGTGGTCACCCAAAGCGACTCAGGCGACGTTATCCAACGCGATGGACGTGAGT‑CAGCCGAACAACTGGCCGCGTGTGGAAGAGTTGTTCCGCCG‑CAAAATCTGGCAACTGAAagag                                                             <  1:748399/239‑1 (MQ=255)

                                                                                                                                                                            aGCGGAACCTTCGGCGTTTGGACGGCGGGTCTGCTGGCGAAGTCA‑CTCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAACGGGAACGATACCGTGCCACGTTTCCTGCACGACGGTCAGTGGTCACCCAAAGCGACTCAGGCGACGTTATCCAACGCGATGGACGTGAGT‑CAGCCGAACAACTGGCCGCGTGTGGAAGAGTTGTTCCGCCG‑CAAAATCTGGCAACTGAAAGAGCt                                                           <  2:143896/239‑1 (MQ=255)

                                                                                                                                                                            aGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCA‑CTCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAACGTGAACGATACCGTGCCACGTTTCCTGCACGACGGTCAGTGGTCACCCAAAGCGACTCAGGCGACGTTATCCAACGCGATGGACGTGAGT‑CAGCCGAACAACTGGCCGCGTGTGGAAGAGTTGTTCCGCCG‑CAAAATCTGGCAACTGAAAGAGCt                                                           <  1:965964/239‑1 (MQ=255)

                                                                                                                                                                                gAACCTTCGGAGAGTTGGCGGCGGGTCTGCTGGCGGAGTCA‑CTGGGTCGGCCGGTGAAACCTTTTATTGCGGCAGCCAACGTGAACGATACCGTGCAGGGTTGCCTGCACGGCGTGCAGTGGTCACCCAAAGCGACTCAGTCGACGTTATCCAACGCGTTGGACGTGAGT‑CAGCCGAACAACTGGCCGCTTGTGGAAGCGTTGTTCCGCCG‑CAAAATCTGGCAACTGAAAGAGCTggg                                                        <  2:355700/238‑1 (MQ=255)

                                                                                                                                                                                    cTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCA‑CTCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAACGTGAACGATACCGTGCCACGTTTCCTGCACGACGGTCAGTGGTCACCCAAAGCGACTCAGGCGACGTTATCCAACGCGATGGACGTGAGT‑CAGCCGAACAACTGGCCGCGTGTGGAAGAGTTGTTCCGCCG‑CAAAATCTGGCAACTGAAAGAGCTGGGTTATg                                                   <  1:496802/239‑1 (MQ=255)

                                                                                                                                                                                    cTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGACC‑CTCGGTCTGCCGGTAAAACGTTTTATTGCTGCGACCAACGTGAACGATACCGTGCCACGTTTCCTGCACGACGGTCAGTGGTCACCCAAAGCGACTCAGGCGACGTTATCCAACGCGATGGACGTGAGT‑CAGCCGAACAACTGGCCGCGTGTGGAAGAGTTGTTCCGCCG‑CAAAATCTGGCAACTGAAAGAGCTGGGTTATg                                                   <  2:506498/239‑1 (MQ=255)

                                                                                                                                                                                     gtgggCGATTGGGCGGGGGGCTGGCTGGCGAGGGCA‑CTCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAACGTGAACGATACCGTGCCACGTTTCCTGCACGACGGTCAGGGGTCGCCCAAAGCGACTCAGGCGACGTTATCCAACGCGATGGACGTGAGT‑CAGCCGAACAACTGGCCGCGTGTGGAAGAGTTGTTCCGCCG‑CAAAATCTGTCAACTGAAAGAGCTGGGTTATg                                                   <  2:1347370/235‑1 (MQ=255)

                                                                                                                                                                                        ggCGATTTGACGGCGGGTCTGCTGGCGAAAGCA‑CTCGGGCTGCCGGCGAAACGGTTTATTTCTGCGGGCAACGTGAACGATACCGTGGCACGGTTCCTGCACGACGGGCAGGGGGGCACCAAAGCGACTCAAGGGGCGGTATCCAACACGCTCGGCGAGAGTACAACGGAACAACT‑GCCGCGGGTGGGAGAGTGGTTCCCCCGCCAACGTTGGGCAAC‑GAAACAGCTGGGGTATGCAg                                                >  2:1214049/1‑238 (MQ=255)

                                                                                                                                                                                           gATTTGACGGGGGGTCTGCTGGCGAAGTTA‑CTCGGTCTGCCGGTCAAACGTTTTATTGCCGCGACCAACGTGAACGATACCGTGCCACGTTTCCTGCACGACGGTCAGTGGTCACCCAAAGCGCCTCAGGCGACGTTATCCAACGCGATGGACGTGAGT‑CAGCCGAACAACTGGCCGCGTGTGGAAGAGTTGTTCCGCCG‑CAAAATCTGGCAACTGAAAGAGCTGGGTTATGCAGCCg                                             <  2:259970/238‑1 (MQ=255)

                                                                                                                                                                                                         tCTGCTGGCGAGGGCA‑CGCGGTCTGCCGGTGAAACGTTTTATTGCTGAGACCAACGTGAACGATACCGTGCCACGTTCCCTGCACGACGGTCAGTGGTCACCCAAAGCGACTCAGGCGACGTTATCCAACGCGATGGACGTGAGT‑CAGCCGAACAACTGGCGGGGTGTGGAAGAGTTGTTCCGCCG‑CAAAATCTGGCAACTGAAAGAGCTGGGTTATGCAGCCGTGGATGATGAAAcc                               <  2:1310481/238‑1 (MQ=255)

                                                                                                                                                                                                         tCTGCTGGCGAAGTCA‑CTCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAACGTGAACGATACCGTGCCACGTTTCCTGCACGACGGTCAGTGGTCACCCAAAGCGACTCAGGCGACGTTATCCAACGCGATGGACGTGAGT‑CAGCCGAACAACTGGCCGCGTGTGGAAGAGTTGTTCCGCCG‑CAAAATCTGGCAACTGAAAGAGCTGGGTTATGCAGCCGTGGATGATGAAAcc                               <  1:1074213/238‑1 (MQ=255)

                                                                                                                                                                                                         gccgctgGCGAAGTCC‑CCCGGTCTGCCGGTGAAACGTTTTATTGCTGCGCCCAACGTGAACGATACCGTGCCACGTTTCCTGCACGACGGTCAGTGGTCACCCAAAGCGACGCAGGCGACGTTATCCAACGCGATGGACGTGAGT‑CAGCCGAACAACTGGCCGCGTGTGGAAGAGTTGTTCCGCCG‑CAAAATCTGGCAACTGAAAGAGCTGGGTTATGCAGCCGTGGATGATGAAAcc                               <  2:821767/235‑1 (MQ=255)

                                                                                                                                                                                                          ctgctgGCGAAGTCA‑CACGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAACGTGAACGATACCGTGCCACGTTTCCTGCACGACGGTCAGTGGTCACCCAAAGCGACTCAGGCGACGTTATCCAACGCGATGGACGTGAGT‑CAGCCGAACAACTGGCCGCGTGTGGAAGAGTTGTTCCGCCG‑CAAAATCTGGCAACTGAAAGAGCTGGGTTATGCAGCCGTGGATGATGAAACCa                              <  1:905677/238‑1 (MQ=255)

                                                                                                                                                                                                           tgctgGCGAAGTCA‑CTCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAACGTGAACGATACCGTGCCACGTTTCCTGCACGACGGTCAGTGGTCACCCAAAGCGACTCAGGCGACGTTATCCAACGCGATGGACTTGAGT‑CAGCCGAACAACTGGCCGCGTGTGGAAGAGTTGTTCCGCCG‑CAAAATCTGGCAACTGa                                                                  <  1:667039/201‑1 (MQ=255)

                                                                                                                                                                                                           tgctgGCGAAATCA‑CTCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAACGTGAACGATACCGTGCCACGTTTCCTGCACGACGGTCAGTGGTCACCCAAAGCGACTCAGGCGACGTTATCCAACGCGATGGACGTGAGT‑CAGCCGAACAACTGGCCGCGTGGGGAAGAGTTGTTCCGCCG‑CAAAATCTGGCAACTGAAAGAGCTTGGTTATGCAGCCGTGGATGAAGAAACCAc                             >  2:984771/1‑238 (MQ=255)

                                                                                                                                                                                                              tgGCGAAGTCC‑CTCGGTCTGCCGGGGAAACGTTTTATTGCGGCGACCAACGGGAACGATACCGTGCCACGTTTCCTGCACGACGGTCAGTGGTCCCCCAAAGCGATTCAGGCGACGTTATCCAACGCGATGGACGTGAGT‑CAGCCGAACAACTGGCCGCGTGTGGAAGAGTTGTTCCGCCG‑CAAAATCTGGCAACTGAAAGAGCTGGGTTATGCAGCCGTGGATGATGAAACCACGCa                          <  2:254095/238‑1 (MQ=255)

                                                                                                                                                                                                              tgGCGAAGTCA‑CTCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAACGTGAACGATACCGTGCCACGTTTCCTGCACGACGGTCAGTGGTCACCCAAAGCGACTCAGGCGACGTTATCCAACGCGATGGACGTGAGT‑CAGCCGAACAACTGGCCGCGTGTGGAAGAGTTGTTCCGCCG‑CAAAATCTGGCAACTGAAAGAGCTGGGTTATGCAGCCGTGGATGATGAAACCACGCaa                         <  2:740642/239‑1 (MQ=255)

                                                                                                                                                                                                              tgGCGAAGTCA‑CTCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAACGTGAACGATACCGTGCCACGTTTCCTGCACGACGGTCAGTGGTCACCCAAAGCGACTCAGGCGACGTTATCCAACGCGATGGACGTGAGT‑CAGCCGAACAACTGGCCGCGTGTGGAAGAGTTGTTCCGCCG‑CAAAATCTGGCAACTGAAAGAGCTGGGTTATGCAGCCGTGGATGATGAAACCACGCaa                         <  1:803635/239‑1 (MQ=255)

                                                                                                                                                                                                               ggCGAAGGCA‑CTCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAACGTGAACGATACCGTGCCACGTTTCCTGCACGACGGTCAGTGGTCACCCAAAGCGACTCAGGCGACGTTATCCAACGCGATGGACGTGAGT‑CAGCCGAACAACTGGCCGCGTGTGGAAGAGTTGTTCCGCCG‑CAAAATCTGGCAACTGAAAGAGCTGGGTTATGCAGCCGTGGATGATGAAACCACGCAAc                        <  1:61040/239‑1 (MQ=255)

                                                                                                                                                                                                                 cGAAGTCA‑CTCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAACGTGAACGATACCGTGCCACGTTTCCTGCACGACGGTCAGTGGTCACCCAAAGCGACTCAGGCGACGTTATCCAACGCGATGGACGGGAGT‑CAGCCGAACAACTGGCCGCGTGTGGAAGAGTTGTTCCGCCG‑CAAAATCTGGCAACTGAAAGAGCTGGGTTATGCAGCCGTGGATGATGAAACCACGCAACa                       <  2:1517198/238‑1 (MQ=255)

                                                                                                                                                                                                                   aaGTCA‑CTCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAACGTGAACGATACCGTGCCACGTTTCCTGCACGACGGTCAGTGGTCACCCAAAGCGACTCAGGCGACGTTATCCAACGCGATGGACGTGAGT‑CAGCCGAACAACTGGCCGCGTGTGGAAGAGTTGTTCCGCCG‑CAAAATCTGGCAACTGAAAGAGCTGGGTTATGCAGCCGTGGATGATGAAACCACGCAACAGAc                    >  1:558453/1‑239 (MQ=255)

                                                                                                                                                                                                                    aGTCA‑CTCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAACGTGAACGATACCGTGCCACGTTTCCTGCACGACGGTCAGTGGTCACCCAAAGCGACTCAGGCGACGTTATCCAACGCGATGGACGTGAGT‑CAGCCGAACAACTGGCCGCGTGTGGAAGAGTTGTTCCGCCG‑CAAAATCTGGCAACTGAAAGAGCTGGGTTATGCAGCCGTGGATGATGAAACCACGCAACAGACa                   <  2:548364/239‑1 (MQ=255)

                                                                                                                                                                                                                          cTCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAACGTGAACGATACCGTGCCACGTTTCCTGCACGACGGTCAGTGGTCACCCAAAGCGACTCAGGCGACGTTATCCAACGCGATGGACGTGAGT‑CAGCCGAACAACTGGCCGCGTGTGGAAGAGTTGTTCCGCCG‑CAAAATCTGGCAACTGAAAGAGCTGGGTTATGCAGCCGTGGATGATGAAACCACGCAACAGACAATGCg              <  1:1107840/239‑1 (MQ=255)

                                                                                                                                                                                                                          cTCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAACGTGAACGATACCGTGCCACGTTTCCTGCACGACGGTCAGTGGTCACCCAAAGCGACTCAGGCGACGTTATCCAACGCGATGGACGTGAGT‑CAGCCGAACAACTGGCCGCGTGTGGAAGAGTTGTTCCGCCG‑CAAAATCTGGCAACTGAAAGAGCTGGGTTATGCAGCCGTGGATGATGAAACCACGCAACAGACAATGCg              <  1:1063755/239‑1 (MQ=255)

                                                                                                                                                                                                                          cTCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAACGTGAACGATACCGTGCCACGTTTCCTGCACGACGGTCAGTGGTCACCCAAAGCGACTCAGGCGACGTTATCCAACGCGATGGACGTGAGT‑CAGCCGAACAACTGGCCGCGTGTGGAAGAGTTGTTCCGCCG‑CAAAATCTGGCAACTGAAAGAGCTGGGTTATGCAGCCGTGGATGATGAAACCACGCAACAGACAATGCg              <  1:999227/239‑1 (MQ=255)

                                                                                                                                                                                                                           tCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAACGTGAACGATACCGTGCCACGTTTCCTGCACGACGGTCAGTGGTCACCCAAAGCGACTCAGGCGACGTTATCCAACGCGATGGACGTGAGT‑CAGCCGAACAACTGGCCGCGTGTGGAAGAGTTGTTCCGCCG‑CAAAATCTGGCAACTGAAAGAGCTGGGTTATGCAGCCGTGGATGATGAAACCACGCAACAGACAATGCGt             >  2:1500253/1‑239 (MQ=255)

                                                                                                                                                                                                                               tCTGCCGGTGAAACGTTTTATTGCTGCGACCAACGTGAACGATACCGTGCCACGTTTCCTGCACGACGGTCAGTGGTCACCCAAAGCGACt                                                                                                                                                               >  1:203107/1‑91 (MQ=255)

                                                                                                                                                                                                                                       tGAAACGTTTTATTGCTGCGACCAACGTGAACGATACCGTGCCACGTTTCCTGCACGACGGTCAGTGGTCACCCAAAGCGACt                                                                                                                                                               >  2:671617/1‑83 (MQ=255)

TCAGGCGCTGGTGAAGCAGGCGTTTGATGATGAAGAACTGAAAGTGGCGCTAGGGTTAAACTCGGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCTGTTGCGCAGCTGCCGCAGGAGACGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCA‑CTCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAACGTGAACGATACCGTGCCACGTTTCCTGCACGACGGTCAGTGGTCACCCAAAGCGACTCAGGCGACGTTATCCAACGCGATGGACGTGAGT‑CAGCCGAACAACTGGCCGCGTGTGGAAGAGTTGTTCCGCCG‑CAAAATCTGGCAACTGAAAGAGCTGGGTTATGCAGCCGTGGATGATGAAACCACGCAACAGACAATGCGTGAGTTAAAAGA  >  NC_000913/4300‑4767

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`