| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 2,040,157 | A→G | 19.4% | K227R (AAG→AGG) | yedY → | membrane‑anchored, periplasmic TMAO, DMSO reductase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,040,157 | 0 | A | G | 19.4% | 117.7 / 4.2 | 61 | K227R (AAG→AGG) | yedY | membrane‑anchored, periplasmic TMAO, DMSO reductase |
| Reads supporting (aligned to +/- strand): ref base A (26/22); new base G (2/11); total (28/33) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.55e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AAGCGTGGTCGATGGTGGTGCCGTGGATTGGTTTTCCGCTGCACAAATTGCTGGCGCTTGCCGAACCTACCAGCAATGCGAAGTATGTCGCTTTCGAAACAATTTATGCACCGGAACAGATGCCAGGCCAGCAGGACCGCTTTATCGGCGGTGGGCTGAAATATCCTTATGTCGAAGGATTGCGTCTCGACGAAGCAATGCATCCGCTCACACTGATGACCGTA‑GGTGTTTATGGCAAGGCGTTACCGCCACAAAATGGCGCGCCGGTGCGACTGATTGTGCC‑GTGGAAATATGGCTTTAAAGGGATTAAATCGATAGTCAGTATTAAGCTGACCCGCGAGCGTCCGCCAACCACCTGGAATCTGGCAGCGCCTGACGAATACGGTTTTTACGCCAACGTTAATCCGTATGTTGATCACCCGCGCTGGTCACAGGCTACCGAACGATTTATTGGTTCAGGCGGCATCCTCGAT > NC_000913/2039920‑2040392 | aaGCGTGGTCGATGGTGGTGCCGTGGATTGGTTTTCCGCTGCACAAATTGCTGGCGCTTGCCGAACCTACCAGCAATGCGAAGTATGTCGCTTTCGAAACAATTTATGCACCGGAACAGATGCCAGGCCAGCAGGACCGCTTTATCGGCGGTGGGCTGAAATATCCTTATGTCGAAGGATTGCGTCTCGACGAAGCAATGCATCCGCTCACACTGATGACCGTA‑GGTGTTTATGGCaa < 1:1157343/238‑1 (MQ=255) gTCGATGGTGGTGCCGTGGATTGGTTTTCCGCTGCACAAATTGCTGGCGCTTGCCGAACCTACCAGCAATGCGAAGTATGTCGCTTTCGAAACAATTTATGCACCGGAACAGATGCCAGGCCAGCAGGACCGCTTTATCGGCGGAGGGCGGTAAAAATCTTATGTCTTAGGATTCTGGTTCGACGGAGCAAGGAAGCCGCCCACACCTTTGAACGTA‑GGTGGTTATTGCTGGGCGGTa > 2:461622/1‑238 (MQ=255) gATGGTGGTGCCGTGGGTTGGTTTTCCGCTGCACAAATTGCTGGCGCTTGCCGAACCTACCAGCAATGCGAAGTATGTCGCTTTCGAAACAATTTATGCACCGGAACAGATGCCAGGCCAGCAGGACCGCTTTATCGGCGGTGGGGTGAAATATCCTTTTGTCGAAGGAGTGCGGCTCGCCGAAGCAAAGCATCCGCTCACACTGATGACCGTT‑GGGGTTTTTTGCAAAGCggttccc > 1:553398/1‑232 (MQ=255) gTGGATTGGTTTTCCGCTGCACAAATTGCTGGCGCTTGCCGAACCTACCAGCAATGCGAAGTATGTCGCTTTCGAAACAATTTATGCACCGGAACAGATGCCAGGCCAGCAGGACCGCTTTATCGGCGGTGGGGTGGAAAATCCTTATTTTGGAGGAGTGCGTCTCGGCGGAGGAAAGCATCCGCTCAAACTGATTACCGCA‑GGTGTTTTTGGCAAAGCGTTACCGCCACaaaaaagcg > 1:1352871/1‑234 (MQ=255) gATTGGTTTTCCGCTGCACAAATTGCTGGCGCTTGCCGAACCTACCAGCAATGCGAAGTATGTCGCTTTCGAAACAATTTATGCACCGGAACAGATGCCAGGCCAGCAGGACCGCTTTATCGGCGGTGGGGTGGAATATTCTTATGTCGAAGGAATGCGGCTCGACGCCACAAAGCCTCCGCTCCCAATGATGAACCTA‑GGTGTTTTTGGGCAGGCGTTACCGCCACAAAAAGgcggg > 1:936689/1‑236 (MQ=255) tGGTTTTCCGCTGCACAATTTGCTGGCGCTTGCCGAACCTCCCAGAAAGGCAAAGTATGTCGTTTTCGAAACAATTTATGCCCCGGAACAGATGCCAGGCAAGGAGGACCGTTTTATCGGGGGGGGGCTAAAATATCCTTATGTCGAAGGATTGCGTCCCGACGAAGCAATGCATCCGCTCACACTGATGCCCGTG‑GGTGTTTATGGCAAGGCTTTACCGCCACAAAATGGCGCGCCgg < 2:553398/239‑1 (MQ=255) ttCCGCTGCACAAATGGCTGGCGCTGCCCGAACCAACCGGCAATGCGAAGTATGTCGCTTTCGAAACAATTTATGCACCGGAACAGATGCCAGGCCGGCAGGCCCGCTTTATCGGCGGTGGGCTGAAATATCCTTATGTCGAAGGATTGCGTCTCGACGAAGCAATGCATCCGCTCACACTGATGCCCGTA‑GGTGTTTATGGCAAGGCGTTACCGCCACAAAATGGCGCGCCGGTGCGa < 1:1007656/239‑1 (MQ=255) acaAATTGCTGGCGCTTGCCGAACCTACCAGAAAGGCGAAGTATGTCGTTTGCGAAACAATTTATGCACCGGAACAGATCCCAGGCCAGCAGGACCGCTTTATCGGCGGTGGGCTGAAATATCCTTATGTCGAAGGATTGCGTCTCGACGAAGCAATGCATCCGCTCACACTGATGACCGTA‑GGTGTTTATGGAAAGGCGTTACCGCCACAAAATGGCGCGCCGGTGCGACTGATtgtg < 1:1429148/239‑1 (MQ=255) aaaatttGCTGGCGCTGCCCAACCCTCCCAGCAATGCGAAGTATGTCGTTTTCAAAAAAATTTATGCACCGGAACAGATGCCAGGCCAGCAGGACCGTTTTAGGGGGGGTGGGCTAAAATATCCTTGTGTCAAAGGATTGCGTCTCGACGAAGCAATGCATCCGCTCACACTGATGACCGTA‑GGTGTTTAGGGCAGGGGGTTACCGCCACAAAATGGCGCGCCGGTGCGACTGATtgtg < 2:983481/234‑1 (MQ=255) aaaaaTTGCTGCCCCTTGCCGAACCACCCAGCAAGGCGAAGGAGGTCGCTTTCGAAACAATTTATGCACCGGAAAAGATGCCAGGCCAGCAGGACCGCTTTACGGGCGGGGGGCTGAAATTTCCTTATGTCGAAGGATGGCGTCTCGACGAAGAAATGCATCCGCTCACACTGATGACCGTG‑GGTGTTTAGGGCAAGGCGTTACCGCCACAAAATGGCGCGCCGGTGCGACTGATtgtg < 2:11378/237‑1 (MQ=255) tGGCGCTTGCCGAACCTACCAGCAATGCGAAGTATGTCGCTTTCGAAACAATTTATGCACCGGAACAGATGCCAGGCCAGCAGGACCGCTTTATCGGCGGTGGGGTGGAATAACCTTTTGTCGAAGGGGTGCGTCTCCGCGAAACAAAGCAACCGCTCCCCCTGCTGACCGGAGGGGGTTTTTTGCAGGGGGTAACC‑CCCCAAAAAAGCGCCCCGGGGCGGCGGATtgtggccggggga > 2:616006/1‑230 (MQ=255) gAACCTACCAGCAATGCGAAGTATGTCGCTTTCGAAACAATTTATGCACGGGAACAGATCCCAGGCCAGCAGGACCGCTTTATCGGCGGTGGGCTGAAATATCCTTATGTCGAGGGTTGGCGTCTCGACGAAGAAATGCACCCGCTCACACTGATGACGGTA‑GGTGTTTATGGCAAGGCGTTACCGCCACAAAATGGCGCGCCGGTGCGACTGATTGTGCC‑GTGGAAATATGGCTTTaa < 2:570277/239‑1 (MQ=255) gAACCTACCAGAAATGCGAAGTATGTCGCTTTCGAAACAATTTATGCACCGGAACAGATGCCAGGCCAGCAGGACCGCTTTATCGGCGGTGGGCTGAAATATCCTTATGTCGAAGGATTGCGTCTCGACGAAGCAATGCACCCGCTCACACTGATGACCGTA‑GGTGTTTAGGGCAAGGCGTTACCGCCACAAAATGGCGCGCCGGTGCGACTGATTGTGCC‑GTGGAAATATGGCTTTaa < 1:616006/239‑1 (MQ=255) aaaCCTACAAGCATGGAGAAGTATGTCGCTTTCAAAAAAATTAATGCACGGGAACAGATGCAGGGCCAGCAGACCCTCTTTACCGGGGGTGGGCTAAAATATCCTTATGTCAAAGGATTGCGTCTCGACGAAGAAATGCATCCGCTCACACGGAGGACCGTG‑GGTGTTTATGGCAGGGGGTTACCGCCACAAAATGGCGCGCCGGTGCGACTGATTGTGCC‑GTGGAAATATGGCTTTaa < 2:549915/238‑1 (MQ=255) cAGCAATGCGAAGTATGTCGCTTTCGAAACAATTTATCCCCCGGAAAAGATGCCAGGCCAGCAGGACCGTTTTATCGGCGGTGGGCTGAAATATCCTTATGTCGAAGGATTGCGTCTCGACGAAGCAATGCATCCGCTCACACTGATGACCGTG‑GGTGTTTATGGCAAGGCGTTACCGCCACAAAATGGCGCGCCGGTGCGACTGATTGTGCC‑GTGGAAATATGGCTTTAAAGGGATTa < 1:944292/239‑1 (MQ=255) tGCGAAGTATGTCGCTTTCGAAACAATTTATGCACCGGAACAGATGCCAGGCCAGCAGGACCGCTTTATCGGCGGTGGGGTGAAAAATCCTTATTTCGAAGGATTTCGGCTCGCCGAAGCAAAGCATCCGCGCACACTGATGACCCCG‑GGGGGTTTTGGGAAAGGGGTAACCCCCCAAAAAAGGGGGCCGGGGGGACTGAGTTTGCCGGGGGAAAAATGGC‑TTAAAGGGGGTAAATAGa > 2:676972/1‑239 (MQ=255) tGCGAAGTATGTCGCTTTCAAAACAATTTATGCACCGGAACAGATGCCAGGCCAGCAGGACCGTTTTATCGGCGGTGGGCTAAAATATCCTTATGTCGAAGGATTGCGTCTCGACGAAGCAATGCATCCGCTCACACTGATGCCCGTG‑GGTGTTTATGGAAAGGCTTTACCGCCACAAAATGGCGCGCCGGTGCGACTGATTGTGCC‑GTGGAAATATGGCTTTAAAGGGATTAAATCGa < 1:1529007/239‑1 (MQ=255) cGAAGTATGTCGCTTTCGAAAAAATTTATGCCCCGAAACAGATGCCAGGCCAGCAGGCCCGTTTTATCGGCGGTGGGCTAAAATATCCTTAGGTCGAAGGATTGCGTCTCGACGAAGCAATGCACCCGCTCACACTGATGCCCGTA‑GGTGTTTAGGGAAAGGCGTTACCGCCACAAAATGGCGCGCCGGTGCGACTGATTGTGCC‑GTGGAAATATGGCTTTAAAGGGATTAAATCGa < 1:213262/237‑1 (MQ=255) gAAGTATGCCGGTTCCGAAATAATTTATCCACCGAAACAGATGCCAGGGCAGCAGGACCGGTTTATGGGCGGGGGCCTGAAATTTCTTTATGCGGAAGGATGGCGTCTGGACGAAGAAATGCACCCGCTCACACTGAGGACCGTG‑GGTGTTTATGGAAGGGCGTTACCGCCACAAAATGGCGCGCCGGTGCGACTGATTGTGCC‑GTGGAAATATGGCTTTAAAGGGATTAAATCGATa < 2:644045/238‑1 (MQ=255) aaGTGTGTGGCTTTAAAACAAATTGTTGCCCCGGAAAAGAGGCCAGGCCAGCAGGCCCCTTTTAGCGGCGGGGGGTTAAAAAATCCTTGTGTCGAGGGTTTGGGTCTCGAGGAAGAAATGCACCCGCTCACTCTGATGACCGTG‑GGTTTTTAGGGCAGGGCGTTACCGCCACAAAATGGCGCGCCGGTGCGACTGATTGTGCC‑GTGGAAATATGGCTTTAAAGGGATTAAATCGATAGt < 2:1359363/239‑1 (MQ=255) aGTATGTCGTTTCCAAAACATTTTTGGCCCCGAAAAAGATGCCGGGCCAGCAGGACCGTTTTATCGGCGGTGGGTTAAAATATCCTTATGTCGAAGGAGTGCGTCTCGACGAAGCAATGCACCCGCTCACACTGATGACCGTG‑GGTGTTTATGGAAAGGCGTTACCGCCACAAAATGGCGCGCCGGTGCGACTGATTGTGCC‑GTGGAAATATGGCTTTAAAGGGATTAAATCGATAGt < 2:686675/238‑1 (MQ=255) tcgcccttcggAAACAATTTAGGCACGGGAACAGACGCAAGGCCCGAAGCTCCGTTTTATCGGGGGTGGGCTGAAATACCCTTATGTCGAAGGATTGCGTCTCGACGAAGCAGTGCTCCCGCCCACACTGATGACGGTG‑GGTGTTTAGGGCAGGGTGTTACCGCCACAAAATGGCGCGCCGGTGCGACTGATTGTGCC‑GTGGAAATATGGCTTTAAAGGGATTAAATCGATAGTCAGTa < 2:759338/229‑1 (MQ=255) tttCGAAACAATTTATGCACCGGAACAGATGCCAGGCCAGCAGGACCGCTTTATCGGCGGTGGGGTGAAATATACTTTAGTCGAAGGAGTGCGTCTCGACGAAGCAATGCATTCGCCCACACTGATGAGCGTA‑GGTGGTTATTGCAAAGGGGTACAGCCCCACAAAGGGGCGCCGGGGCGGCTGGGTGTGTC‑GGGGGAATATGGCTTTTAAGGGGTTAAAAAGATAGACAGAATTaa > 2:448727/1‑237 (MQ=255) tttCAAAACAATTTATGCACCGGAACAGATGCCAGGCCAGCGGGACCGTTTTACCGGCGGTGGGTTGAAATATCCTTATGTCGAAGGATGGCGTCTCGACGAAGCAATGCATCCGCTCACACTGATGACCGTA‑GGTGTTTATGGCAAGGCTTTACCGCCACAAAATGGCGCGCCGGTGCGACTGATTGTGCC‑GTGGAAATATGGCTTTAAAGGGATTAAATCGATAGTCAGTATTAAGc < 1:1224109/239‑1 (MQ=255) tCGAAACAATTTATGCACCGGAACAGATGCCAGGCCAGCAGGACCGCTTTATCGGCGGTGGGCTGCAAAATTCTTATTTCGAAAGGTTTCCTTTCGACGGAGCAATGCCTCCGCCCACACTGATGACCGTT‑GGGGGTTTTTGCAAGGCGTTACCACCACCAAAACGCGCGCCGGGGGGGATGATTGTGCC‑GTGGGAATATGGGTTTAAAAGGAGTAAATAGCTAATCAGTGTTaaattt > 2:764446/1‑235 (MQ=255) tttATGCCCCGGAACAGATGCCAGGCCAGCAGGACCGCTTTATCGGCGGGGGGCTGAAATATCCTTATGTCGAAGGATTGCGTCTCGACGAAGCAATGCATCCGCTCACACTGATGACCGTA‑GGTGTTTATGGAAAGGCGTTACCGCCACAAAATGGCGCGCCGGTGCGACTGATTGTGCC‑GTGGAAATATGGCTTTAAAGGGATTAAATCGATAGt < 1:543736/217‑1 (MQ=255) cGGAACAGATGCCAGGACAGCAGGTCCGCTTTATCGGCGGTGGGCTGAAATACCCTTATGTCGAAGGATTGCGTCTCGACGAAGCAATGCACCCGCTCACCCTGATGACCGTA‑GGTGTTTATGGCAAGGCTTTACCGCCACAAAATGGCGCGCCGGTGCGACTGATTGTGCC‑GTGGAAATATGGCTTTAAAGGGATTAAATCGATAGt < 1:739785/208‑1 (MQ=255) aaCAGATGCCAGGCCAGCAGGACCGTTTTATCGGCGGTGGGCTGAAATATCCTTATGTCGAAGGATTGCGTCTCGACGAAGCAATGCATCCGCTCACACTGATGCCCGTA‑GGTGTTTATGGCAGGGCTTTACCGCCACAAAATGGCGCGCCGGTGCGACTGATTGTGCC‑GTGGAAATATGGCTTTAAAGGGATTAAATCGATAGTCAGTATTAAGCTGACCCGCGAGCGTCCGCCAac < 1:1263253/238‑1 (MQ=255) caggcagGACCGCTTTTCCGGGGGTGGGCTGAAATATCTTTATGCCGAAGGTTTGCGTCTCGACGAAGCAATGCATCCGCTCACACTGATGACCGTA‑GGTGTTTATGGCAAGGCTTTACCGCCACAAAATGGCGCGCCGGTGCGACTGATTGTGCC‑GTGGAAATATGGCTTTAAAGGGATTAAATCGATAGTCAGTATTAAGCTGACCCGCGAGCGTCCGCCAACCACCTGGAATCTgg < 1:936154/236‑1 (MQ=255) cagGACCGCTTTATCGGCGGTGGGGTGAAATATCCTTATGTCGAAGGATTGCGTCTCGACGAAGAAATGCATCCCCTCCCCCTGATGACCGCA‑GGGGTTTTTTGCAAGGCGGTACCGCCCCCAAAAGGCGCGCCCGGGCGGCCGCTTGTGCC‑GTGGGAATATTGCTTTTAAGGGGTTAAATCGATAGGTAGTATTAAACTGACCCGCGCGCGTCCCCCCACCACCCGGAATCttggtgc > 2:480151/1‑233 (MQ=255) cgttttACCGGGGGGGGTCTGAAATATCCTTATGTCGAAGGATTGCGTCTCGACAAAGCAATGCACCCGCTCACACTGATGCCCGTG‑GGTGTTTATGAAAAGCGGTTACCGCCACAAAATGGCGCGCCGGTGCGACTGATTGTGCC‑GTGGAAATATGGCTTTAAAGGGATTAAATCGATAGTCAGTATTAAGCTGACCCGCGAGCGTCCGCCAACCACCTGGAATCTGGCAGCGCCTGa < 1:1076157/236‑1 (MQ=255) tGGGCTAAAATATCCTTATGTCGAAGGATTGCGTCTCGACGAAGCAATGCATCCGCTCACACTGATGACCGTA‑GGTGTTTATGGCAAGGCGTTACCGCCACAAAATGGCGCGCCGGTGCGACTGATTGTGCC‑GTGGAAATATGGCTTTAAAGGGATTAAATCGATAGTCAGTATTAAGCTGACCCGCGAGCGTCCGCCAACCACCTGGAATCTGGCAGCGCCTGACGAATACGGtttt < 1:545712/238‑1 (MQ=255) atCCTTATGTGGAAGGATTGCTTCCCGACGAAGAAATGCATCCGCTCACACTGATGACGGTA‑GGTGTTTATGGCAAGGCGTTACCGCCACAAAATGGCGCGCCGGTGCGACTGATTGTGCC‑GTGGAAATATGGCTTTAAAGGGATTAAATCGATAGTCAGTATTAAGCTGACCCGCGAGCGTCCGCCAACCACCTGGAATCTGGCAGCGCCTGACGAATACGGTTTTTACGCCAACGtt < 1:461622/239‑1 (MQ=255) atCCTTATGTCGAAGGATGGGGCCTCGAAGAGGCAATGCACCCGCTCACCCGGATGGCGGTG‑GGTGTTTTGGGCAGGGCTTTACCGCCACAAAATGGCGCGCCGGTGCGACTGATTGTGCC‑GTGGAAATATGGCTTTAAAGGGATTAAATCGATAGTCAGTATTAAGCTGACCCGCGAGCGTCCGCCAACCACCTGGAATCTGGCAGCGCCTGACGAATACGGTTTTTACGCCAACGtt < 2:936689/239‑1 (MQ=255) acccTTATTGCGAAGGATTGCGTCGCGACGAAGCAATGCATCCGCTCACACTGATGACCGTA‑GGTGTTTAGGGCAAGGCGTTACCGCCACAAAATGGCGCGCCGGTGCGACTGATTGTGCC‑GTGGAAATATGGCTTTAAAGGGATTAAATCGATAGTCAGTATTAAGCTGACCCGCGAGCGTCCGCCAACCACCTGGAATCTGGCAGCGCCTGACGAATACGGTTTTTACGCCAACGtt < 1:480151/237‑1 (MQ=255) tCCTTATGTCGAAGGATTGCGTCTCGACGAAGCAATGCATCCGCTCACACTGATGACCGTA‑GGTGTTTATGGCAAGGCGTTACCGCCACAAAATGGCGCGCCGGTGCGACTGATTGTGCC‑GTGGAAATATGGCTTTAAAGGGATTAAATCGATAGTCAGTATTAAGCTGACCCGCGAGCGTCCGCCAACCACCTGGAATCTGGCAGCGCCTGACGAATACGGTTTTTACGCCAACGTTa > 1:1060553/1‑239 (MQ=255) tCCTTATGTCGAAGGATTGCGTCTCGACGAAGCAATGCATCCGCTCACACTGATGACCGTA‑GGTGTTTATGGCAAGGCGTTACCGCCACAAAATGGCGCGCCGGTGCGACTGATTGTGCC‑GTGGAAATATGGCTTTAAAGGGATTAAATCGATAGTCAGTATTAAGCTGACCCGCGAGCGTCCGCCAACCACCTGGAATCTGGCAGCGCCTGACGAATACGGTTTTTACGCCAACGTTa > 2:37631/1‑239 (MQ=255) tCCTTATGTCGAAGGATTGCGTCTCGACGAAGCAATGCATCCGCTCACACTGATGACCGTA‑GGTGTTTATGGCAAGGCGTTACCGCCACAAAATGGCGCGCCGGTGCGACTGATTGTGCC‑GTGGAAATATGGCTTTAAAGGGATTAAATCGATAGTCAGTATTAAGCTGACCCGCGAGCGTCCGCCAACCACCTGGAATCTGGCAGCGCCTGACGAATACGGTTTTTACGCCAACGTTa > 2:1358750/1‑239 (MQ=255) cTTATGTCGAGGGATTGCGTCCCGACGAAGCAATGCACCCGCTCACACTGATGACCGGA‑GGTTTTTATGGCAAGGCGTTACCGCCACAAAATGGCGCGCCGGTGCGACTGATTGTGCC‑GTGGAAATATGGCTTTAAAGGGATTAAATCGATAGTCAGTATTAAGCTGACCCGCGAGCGTCCGCCAACCACCTGGAATCTGGCAGCGCCTGACGAATACGGTTTTTACGCCAACGTTAAt < 2:239051/239‑1 (MQ=255) cTTATGTCGAAGGATTGCGTCCCGACGAAGCAATGCATCCGCTCACACTGATGACCGTA‑GGTGTTTATGGCAAGGCGTTACCGCCACAAAATGGCGCGCCGGTGCGACTGATTGTGCC‑GTGGAAATATGGCTTTAAAGGGATTAAATCGATAGTCAGTATTAAGCTGACCCGCGAGCGTCCGCCAACCACCTGGAATCTGGCAGCGCCTGACGAATACGGTTTTTACGCCAACGTTAAt < 1:764446/239‑1 (MQ=255) aTGTCGAAGGATTGCGTCTCGACGAAGCAATGCATCCGCTCACACTGATGACCGTA‑GGTGTTTATGGCAAGGCGTTACCGCCACAAAATGGCGCGCCGGTGCGACTGATTGTGCC‑GTGGAAATATGGCTTTAAAGGGATTAAATCGATAGTCAGTATTAAGCTGACCCGCGAGCGTCCGCCAACCACCTGGAATCTGGCAGCGCCTGACGAATACGGTTTTTACGCCAACGTTAATCCg > 2:820549/1‑239 (MQ=255) tCGAAGGATTGCGTCTCGACGAAGCAATGCATCCGCTCACACTGATGACCGTA‑GGTGTTTATGGCAAGGCGTTACCGCCACAAAATGGCGCGCCGGTGCGACTGATTGTGCC‑GTGGAAATATGGCTTTAAAGGGATTAAATCGATAGTCAGTATTAAGCTGACCCGCGAGCGTCCGCCAACCACCTGGAATCTGGCAGCGCCTGACGAATACGGTTTTTACGCCAACGTTAATCCGTAt > 1:543419/1‑239 (MQ=255) gAAGGCTTGCGTCTCGACGAAGCAATGCATCCGCTCACACTGATGACCGTA‑GGAGTTTATGGCAAGGCGTTACCGCCACAAAATGGCGCGCCGGAGCGACTGATTGTGCC‑GTGGAAATATGGCTTTAAAGGGATTAAATCGATAGTCGGTATTAAGCTGACCCGCGAGCGTCCGCCAACCACCTGGAATCTGGTAGCGCCTGACGAATACGGTTTTTACGCCAACGTTAATCCGTATGt > 2:572198/1‑239 (MQ=255) gAAGGATTGCGTCTCGACGAAGCAATGCATCCGCTCACACTGATGACCGTA‑GGTGTTTATGGCAAGGCGTTACCGCCACAAAATGGCGCGCCGGTGCGACTGATTGTGCC‑GTGGAAATATGGCTTTAAAGGGATTAAATCGATAGTCAGTATTAAGCTGACCCGCGAGCGTCCGCCAACCACCTGGAATCTGGCAGCGCCTGACGAATACGGttttt > 1:857365/1‑217 (MQ=255) aaGGATTGCGTCTCGACGAAGCAATGCATCCGCTCACACTGATGACCGTA‑GGTGTTTATGGCAAGGCGTTACCGCCACAAAATGGCGCGCCGGTGCGACTGATTGTGCC‑GTGGAAATATGGCTTTAAAGGGATTAAATCGATAGTCAGTATTAAGCTGACCCGCGAGCGTCCGCCAACCACCTGGAATCTGGCAGCGCCTGACGAATACGGTTTTTACGCCAACGTTAATCCGTATGtt > 1:99378/1‑239 (MQ=255) tGCGTCTCGACGAAGCAATGCATCCGCTCACACTGATGACCGTA‑GGTGTTTATGGCAAGGCGTTACCGCCACAAAATGGCGCGCCGGTGCGACTGATTGTGCC‑GTGGAAATATGGCTTTAAAGGGATTAAATCGATAGTCAGTATTAAGCTGACCCGCGAGCGTCCGCCAACCACCTGGAATCTGGCAGCGCCTGACGAATACGGTTTTTACGCCAACGTTAATCCGTATGTTGATCa > 2:1425473/1‑238 (MQ=255) ctggaggaAGCAATGCATCCGCTCACACGGATGACGGTG‑GGTGTTTAGGGCAGGGCGTTACCGCCACAAAATGGCGCGCCGGTGCGACTGATTGTGCC‑GTGGAAATATGGCTTTAAAGGGATTAAATCGATAGTCAGTATTAAGCTGACCCGCGAGCGTCCGCCAACCACCTGGAATCTGGCAGCGCCTGACGAATACGGTTTTTACGCCAACGTTAATCCGTATGTTGATCACCcgc < 2:329873/232‑1 (MQ=255) aaTGCATCCGCTCACAGTGATGACCGTA‑GGTGTTTATGGCAGGGCGTTACCGCCACAAAATGGCGCGCCGGTGCGACTGATTGTGCC‑GTGGAAATATGGCTTTAAAGGGATTAAATCGATAGTCAGTATTAAGCTGACCCGCGAGCGTCCGCCAACCACCTGGAATCTGGCAGCGCCTGACGAATACGGTTTTTACGCCAACGTTAATCCGTATGTTGATCACCCGCGCTGGTcaca < 1:1351161/237‑1 (MQ=255) aaTGCACCCGCCCACACTGATGCCGGTG‑GGTGTTTAGGGCAAGGCGTTACCGCCACAAAATGGCGCGCCGGTGCGACTGATTGTGCC‑GTGGAAATATGGCTTTAAAGGGATTAAATCGATAGTCAGTATTAAGCTGACCCGCGAGCGTCCGCCAACCACCTGGAATCTGGCAGCGCCTGACGAATACGGTTTTTACGCCAACGTTAATc < 2:785187/209‑1 (MQ=255) ccGCTCACACTGATGACCGTA‑GGTGTTTATGGCAAGGCGTTACCGCCACAAAATGGCGCGCCGGTGCGACTGATTGTGCC‑GTGGAAATATGGCTTTAAAGGGATTAAATCGATAGTCAGTATTAAGCTGACCCGCGAGCGTCCGCCAACCACCTGGAATCTGGCAGCGCCTGACGAATACGGTTTTTACGCCAACGTTAATCCGTATGTTGATCACCCGCGCTGGTCACAGGCTACCGa > 1:635643/1‑239 (MQ=255) cTCACACTGATGACCGTA‑GGTGTTTATGGCAAGGCGTTACCGCCACAAAATGGCGCGCCGGTGCGACTGATTGTGCC‑GTGGAAATATGGCTTTAAAGGGATTAAATCGATAGTCAGTATTAAGCTGACCCGCGAGCGTCCGCCAACCACCTGGAATCTGGCAGCGCCTGACGAATACGGTTTTTACGCCAACGTTAATCCGTATGTTGa > 1:785187/1‑209 (MQ=255) acTGTTGACCGTA‑GGTGTTTATGGCAAGGCGTTACCGCCACAAAATGGCGCGCCGGTGCGATTGATTGTGTT‑GTGTAAATATGGCTTTAAAGGGATTAAATCGATAGTCAGTATTAAGCTGACCCGCGAGCGCCCGCCAACCACCTGGAATGTGTCAGCGCCTGACGAATACGGGTTTTACGCCAACGTTAATTCGTTTGCTGATCTACCGCGCTGGACACCGGCTTCAGTACGATTTa > 2:1178374/1‑239 (MQ=255) gTA‑GGTGTTTATGGCAAGGCGTTACCGCCACAAAATGGCGCGCCGGTGCGACTGATTGTGCC‑GTGGAAATATGGCTTTAAAGGGATTAAATCGATAGTCAGTATTAAGCTGACCCGCGAGCGTCCGCCAACCACCTGGAATCTGGCAGCGCCTGACGAATACGGTTTTTACGCCAACGTTAATCCGTATGTTGATCACCCGCGCTGGTCACAGGCTACCGAACGATTTATTGGTTCAg > 2:1056412/1‑238 (MQ=255) gTA‑GGTGTTTATGGCAAGGCGTTACCGCCACAAAATGGCGCGCCGGTGCGACTGATTGTGCC‑GTGGAAATATGGCTTTAAAGGGATTAAATCGATAGTCAGTATTAAGCTGACCCGCGAGCGTCCGCCAACCACCTGGAATCTGGCAGCGCCTGACGAATACGGTTTTTACGCCAACGTTAATCCGTATGTTGATCACCCGCGCTGGTCACAGGCTACCGAACGATTTATTGGTTCAg > 1:451451/1‑238 (MQ=255) tgttttttAGGACAGGGCGTAACCGCCACAAAATGGCGCGCCGGTGCGACTGATTGTGCC‑GTGGAAATATGGCTTTAAAGGGATTAAATCGATAGTCAGTATTAAGCTGACCCGCGAGCGTCCGCCAACCACCTGGAATCTGGCAGCGCCTGACGAATACGGTTTTTACGCCAACGTTAATCCGTATGTTGATCACCCGCGCTGGTCACAGGCTACCGAACGATTTATTGGTTCAggc < 2:101421/233‑1 (MQ=255) gtgtTTATGGCAAGGCGTTACCGCCACAAAATGGCGCGCCGGTGCGACTGATTGTGCC‑GTGGAAATATGGCTTTAAAGGGATTAAATCGATAGTCAGTATTAAGCTGACCCGCGAGCGTCCGCCAACCACCTGGAATCTGGCAGCGCCTGACGAATACGGTTTTTACGCCAACGTTAATCCGTATGTTGATCACCCGCGCTGGTCACAGGCTACCGAACGATTTATTGGTTCAggcggc > 2:1349740/1‑239 (MQ=255) gtgtTTATGGCAAGGCGTTACCGCCACAAAATGGCGCGCCGGAGCGACTGATTGTGCC‑GTGGAAATATGGCTTTAAAGGGATTAAATCGATAGTCAGAATTAAGCTGACCCGCGAGCGTCCGCCAACCACCTGGAATCTGGCAGCGCCTGACGAATACGGTTTTTACGCCAACGTTAATCCGTATGTTGATCACCCGCGCTGGTCACAGGCTACCGAACGATTTATTGGTTCAggcggc > 1:950138/1‑239 (MQ=255) ttttATGGAAGGAGGTTACCGCCACAAAATGGCGCGCCGGTGCGACTGATTGTGCC‑GTGGAAATATGGCTTTAAAGGGATTAAATCGATAGTCAGTATTAAGCTGACCCGCGAGCGTCCGCCAACCACCTGGAATCTGGCAGCGCCTGACGAATACGGTTTTTACGCCAACGTTAATCCGTATGTTGATCACCCGCGCTGGTCACAGGCTACCGAACGATTTATTGGTTCAGGCGGCAt < 2:35808/238‑1 (MQ=255) tATGGCAAGGCGTTACCGCCACAAAATGGCGCGCCGGTGCGACTGATTGTGCC‑GTGGAAATATGGCTTTAAAGGGATTAAATCGGTAGTCAGTATTAAGCTGACCCGCGAGCGTCCGCCAACCACCTGGAATCTGGCAGCGCCTGACGAATACGGTTTTTACGCCAACGTTAATCCGTATGTTGATCACCCGCGCTGGTAACAGGCTACCGAACGATTTATTGGTTCAGGCGGCATcc > 2:248011/1‑238 (MQ=255) tGGAAAGGTGTTACCGCCACAAAATGGCGCGCCGGTGCGACTGATTGTGCC‑GTGGAAATATGGCTTTAAAGGGATTAAATCGATAGTCAGTATTAAGCTGACCCGCGAGCGTCCGCCAACCACCTGGAATCTGGCAGCGCCTGACGAATACGGTTTTTACGCCAACGTTAATCCGTATGTTGATCACCCGCGCTGGTCACAGGCTACCGAACGATTTATTGGTTCAGGCGGCATCCTCg < 1:874395/239‑1 (MQ=255) ggCAAGGCGTTACCGCCACAAAATGGCGCGCCGGTGCGACTGATTGTGCC‑GTGGAAATATGGCTTTAAAGGGATTAAATCGATAGTCAGTATTAAGCTGACCCGCGAGCGTCCGCCAACCACCTGGAATCTGGCAGCGCCTGACGAATACGGTTTTTACGCCAACGTTAATCCGTATGTTGATCACCCGCGCTGGTCACAGGCTACCGAACGATTTATTGGTTCAGGCGGCATCCTCGa > 2:1075482/1‑239 (MQ=255) gCAAGGCGTTACCGCCACAAAATGGCGCGCCGGTGCGACTGATTGTGCC‑GTGGAAATATGGCTTTAAAGGGGTTAAATCGATAGTCAGTATTAAGCTGACCCGCGAGCGTCCGCCAACCACCTGGAATCTGGCAGCGCCTGACGAATACGGTTTTTACGCCAACGTTAATCCGTATGTTGATCACCCGCGCTGGTCACAGGCTACCGAACGATTTATTGGTTCAGGCGGCATCCTCGAt > 2:789088/1‑239 (MQ=255) | AAGCGTGGTCGATGGTGGTGCCGTGGATTGGTTTTCCGCTGCACAAATTGCTGGCGCTTGCCGAACCTACCAGCAATGCGAAGTATGTCGCTTTCGAAACAATTTATGCACCGGAACAGATGCCAGGCCAGCAGGACCGCTTTATCGGCGGTGGGCTGAAATATCCTTATGTCGAAGGATTGCGTCTCGACGAAGCAATGCATCCGCTCACACTGATGACCGTA‑GGTGTTTATGGCAAGGCGTTACCGCCACAAAATGGCGCGCCGGTGCGACTGATTGTGCC‑GTGGAAATATGGCTTTAAAGGGATTAAATCGATAGTCAGTATTAAGCTGACCCGCGAGCGTCCGCCAACCACCTGGAATCTGGCAGCGCCTGACGAATACGGTTTTTACGCCAACGTTAATCCGTATGTTGATCACCCGCGCTGGTCACAGGCTACCGAACGATTTATTGGTTCAGGCGGCATCCTCGAT > NC_000913/2039920‑2040392 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |