Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,151,691 C→T 19.1% intergenic (+45/+20) yegJ → / ← yegK uncharacterized protein/ser/thr phosphatase‑related protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,151,6910CT19.1% 77.7 / 4.8 50intergenic (+45/+20)yegJ/yegKuncharacterized protein/ser/thr phosphatase‑related protein
Reads supporting (aligned to +/- strand):  ref base C (23/17);  new base T (6/4);  total (29/21)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.90e-01

TATTGTTACAAACGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTATACCATTGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTGAATTGTTGTCTTCAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGCATATTTAACGCCAGTCGCTGGATACCATCCGTA  >  NC_000913/2151475‑2151921
                                                                                                                                                                                                                            |                                                                                                                                                                                                                                         
tATTGTTAAAAACGGAGAATAGGGCAGGGAGTGAAAAAAAAAGAAAAAAGATATTTCAGTCTGGATTTATACGGGGGGGAAAAAAATTTGCGGTGTTTATACCATTGATCTTTTATTGGTCACTTATCAAAAAAAAGAAGCCGACGAGAAAAGAGAAAAATTGGTTCGCTAACCCGCCTTTATATTTATTGTTAAAACGGGCGGTTGAATTGTTGTCTTCAACCGCCATGAGAAactc                                                                                                                                                                                                                    <  2:939130/238‑1 (MQ=255)
 aTTGTTACAAACGTAGAATATGGCGAGGAGGGAAAAAAAAAAAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGAATAAAATTTACGGCGGTTATCCCTTTGATCTTTTATTGGTCACCTATCAAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGTTAACCAGCCGTTATATTTATTGTTTAAACGGGCGGTTGAATTGTTTTTTTCAACCGCCATGAGAAactcac                                                                                                                                                                                                                  <  1:842872/239‑1 (MQ=255)
                  atatGGCCGGGGGTGAAAAAAAAAAAAAGAAGATATTCCAGACTGGATTTATACGCGCGGGGATAAAATTTACGGGGGTTATACAATTAATCTTTTATTGGTCACCTACCAAAAAGAAGAAGCCGACGAGCTAAGAGAAAAATTAGTTCCTTAATCAGCCTTTATATTTATTGTTAAACCGGGCGGTTAAATTTTTGTCTTCAACCGCCATGAGAAACTCACTCTGCCCACAGCGcc                                                                                                                                                                                                   <  1:1370609/237‑1 (MQ=255)
                     tGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTATACCATTTATCCTTTTTTTGTCACCTATTCAAAAAAAGAAGACGACGAGCTAAGAGCAAAAATAGTTTGCTTATTAGACGTTATATTTATTTTTTAAAATAGCGGGTGTATTTTTTTTTTTTAACACCCTTGAAAAAACACACTTTCCCCACAGCCCCAg                                                                                                                                                                                                 >  1:404175/1‑238 (MQ=255)
                         cAGGAGTGGAAAAAAAAAAAGAAAGTTTTTTCAGCCGGGATGTATCCGCGGGGGAAAAAATTTACCGGGTGTTATCCCATTGTTCCTTTATGGGTCCCCTACCAAAAAGAAGAACCCAACGAGTTAAGAGAAAAATTGTTCCGCTAATAAGCGTTTATTTTTATTGTTTAAACGGGGGGTTAAATTGTTGTCTTCAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCaa                                                                                                                                                                                           <  1:501181/238‑1 (MQ=255)
                             aGTGGAAAAAAAAGAAAAAAGATTTTACACTCTGTATGTATACGCGGTGGAAAAAATTTTACGGGGTTTATCCCATTGATCTTTTTTGGTCCCCCTATCAAAAAAAAGAGGCCCGGGAGCAAAGAAAAAAATTTTTTCGTAAACCACCCTTTATATTTTTTTTTTAAACGGGGGGTGAAATTGTTTTCTTCAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGtt                                                                                                                                                                                       <  2:1073912/238‑1 (MQ=255)
                                                      ttCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTATACCATTGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTGAATTGTTGTCtt                                                                                                                                                                                                                                         <  1:797680/165‑1 (MQ=255)
                                                               gttGTATACGCGGGGGGAAAAAATTTCCGGGGTTTACACTGTTGATCTTTTTTGGGTAACATATCAAAAAGAAGAAGCCGACGAGTAAAAATAAAAATTATTTCCATAAACATCTTTTATATTTATTTTTAAAACTGGGGGTTTAATTCTTTCCCTAATCCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGc                                                                                                                                                     <  1:1296637/236‑1 (MQ=255)
                                                                       cgcgggGGGATAAAATTTACGGGGTTTATCCCATTGATCTTTTATTGGTCACTTATCAAAAAGAAGAAGCCGACGATCTAAGAGAAAAATTATTTCGCAAATCAGCCGTTATATTTTTTGTTAAACCGGGGGGTTAATTTGTTTTCTTCAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACaaaa                                                                                                                                            <  1:507052/239‑1 (MQ=255)
                                                                       cgcgggGGAAAAAAATTTACGGCGGTTATACCTTTTATCCTTTTTGGCTCCCCTCCCAAAAAAAAAAAGCCGCCGATCAAAGAGAAAAATTGGTTCGCTAATCAGCCTTTATATTTTTTTTTTAACCGGGGGGTTGATTTTTTGTCTTCAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACaaaa                                                                                                                                            <  1:1475065/239‑1 (MQ=255)
                                                                                       ttACGGGGTTTATACATTTGATCTTTTATTGGTCCCCTATCAAAAAGAAAAAGCCGAAGAGCTAAGAGAAAAATTATTCCGCTAATCAGCGTTTATTTTTTTTTTTTAAACGGGCGTTTGAATTGTTGTCTTCAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTc                                                                                                                           <  1:1417096/239‑1 (MQ=255)
                                                                                       ttACGGGGGTTAAACAATTGATCTTTTTTTGGTCCCCTACCAAAAAAAAGAAGCCGACGAGAAAAGAAAAAAATTATTCCGTTAACCAGCTGTTATTTTTATTTTTAAAACGGGGGTTTGATTTTTTGTTTTCAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTc                                                                                                                           <  1:762124/239‑1 (MQ=255)
                                                                                        tacgggaggTTATAACTTTGATCTTTTATTGGTCCCCTAACAAAAAAAAAAACCCGACGAGTAAAGAAAAAAATTAGTTCGCTAACCAGCCTTTATTTTTTTTGTTAAACCGGGCGGTTGAATTGTTGTCTCCAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTc                                                                                                                           <  1:30365/232‑1 (MQ=255)
                                                                                                tttATACCTTTTATCCTTTTTTGGTCCCCTATCAAAAAAAAAAACCGAACGAGCTAAGAAAAAAATTTGTTCGTTAATCACCCTTTTTTTTTTTTTTTAAAACGGGGGTTTGAATTTTTGTTTCCAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGtaat                                                                                                                    <  1:1409538/237‑1 (MQ=255)
                                                                                                        aTTTATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGGTTTAATTTTGTTTTTCAACCCCCTTAGGAAAACAACCTTCCCCCCACGCCCCGGGCCACCGTTTTTTCATATACCTTACCCCCGTTTCCCCCGCGGCTGGGCAAAAACTCTTTTAAAAATTCCgggataaaaat                                                                                                               >  2:254185/1‑227 (MQ=255)
                                                                                                          tGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTGGATTTTTTTTTTCCACCCGCATTAAAAAATCACTCTTCCCACAACGCCAGTGCCAACGGTTTATCATCATCCCAACCCCCGCTTACCGCCGGGCTGGGCAAAAACTTCTTTTACCATTTCAGGAATTAAATAGAgtt                                                                                                          >  2:1125891/1‑239 (MQ=255)
                                                                                                          tGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTGAATTGTTGTCTTTAACCGCCATGAGAAAATAACTCTGCCCACAGCGCCAGTGCCAACGTTTTTTCATCATCCGTACGCCCCTTTACCCCCGGGCTGGGCAAAAACTGCTTTAACAATTCAGGGAATAAATCGAgtta                                                                                                         >  2:87208/1‑238 (MQ=255)
                                                                                                          tGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTGAATTGTTGTCTTCTACCGCCAAGAGAAACTCACTCTCCCCACCGCGCCACTGCCCACGTTTTATCATCATCCCTACGCTCGTTTAACCCCGCGCTGGGCCACAACCTCTTTTACAATTAAGGTAATAAAACCAgttt                                                                                                         >  2:1402626/1‑238 (MQ=255)
                                                                                                           gATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTTAATTGTTTTCTTCAACCGCCATGAGAAAATCACTCTGCCCACAGCGCCAGTGCCAACCTTTTTTCATCATCCGTATGCCCCCTTACCGCCCGGCTGGACCAAAACTGCTTTTACAATTTAGGTCATAAATCGtgttgt                                                                                                        >  1:842855/1‑239 (MQ=255)
                                                                                                           gATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTGAATTGTTGTCTTCAACCGCCATGAGAAACTCACTCTGCCCACAACGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAgttgt                                                                                                        >  1:1218018/1‑239 (MQ=255)
                                                                                                           gATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTGAATTGTTGTCTTCAACCGCCATGAGAAAATCACTCTGCCCACAGCGCCACGGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGGCAAAAACTGCTTTAACAATTCAGGTAATAAATCGAgttgt                                                                                                        >  1:620733/1‑239 (MQ=255)
                                                                                                            aTCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTGAATTGTTGTCTTCCACCCCCCTGAGAAAATCACTCTGCCCCCAGCGCCAGTGCCAACGTTTTATCATCATCCCTACGCTCGTTTACCGCCGGGCTGGACAAAAAATGCTTTAACAATTCCGGTAATAAATCGAgttggt                                                                                                       >  2:993269/1‑237 (MQ=255)
                                                                                                              ccTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTGAATTTGTGTTTTCAACCGCCATGAGAAAACCCCTCTGCCCACAGCGCCCGTGCCAACGGTTTATCATCATCCGGACGCTCGTTTACCCCCGGGCTGGGCCAAAACTGCTTTAACAAATCAAGGAATAAATCGAggtttt                                                                                                       >  2:586337/1‑232 (MQ=255)
                                                                                                                   aTTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTGCATTTTTTTCTTCAACCGCCCTGAGAAACTCACTCTGCCCCCAGCGCCAGCGCCCACGCTTTATCATCCTCCGCACCCCCGTTTACCGCCCGGCTGGCCAAAAACTGCTCTAACAATTCACGCAATAAATCGCGTTCTTCCTGCgg                                                                                                >  2:710382/1‑238 (MQ=255)
                                                                                                                       gTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTGGATTGTTGTCTTCAACCGCCCTTAGAAACTCACTCTGCCCAACGCGCCAGTGCCAACGTTTTATCACCCTCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTTACAATTCTGGGAATAAATCGAGTTGTTCCTGCGtt                                                                                               >  2:360203/1‑236 (MQ=255)
                                                                                                                       gTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTGAATTGTTTTTTTTCACCGCCGTAAGGAACTCACTCTTCCCACAGCGCCAGTGCCAACGTTTTTTCAACCACCGTACCCTCGTTTTCCGCCGGGCCGGGCAAAAACCGCTTTATCAATTTAGGTCATAAAACGAGCTGTTCCTGCgattca                                                                                            >  2:64607/1‑234 (MQ=255)
                                                                                                                         caataaataaaaaaaaaaaaGCCGAGAAGAAAAGAAAAAAATTTGTTCGATAAGCATCTGTTATATTTATTGTATAAACGGGGGGTTAGATTTTTGTCAAAAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTtgct                                                                                            <  2:620733/228‑1 (MQ=255)
                                                                                                                           aCCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTGAATTGTTGTCTTCTACCGCCATTAGAAACTCACTCTGCCCACAACGCCCATGCCAACGCTTTTTCATCATCCGTAAGCCCGTTTTCCGCCCGGCTGGGCCAAAACTTCTTTAACCATTTAGGTAATAAATCGAGTTGTTTCCTCGTtgctgc                                                                                          >  2:488447/1‑238 (MQ=255)
                                                                                                                           aCCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTGAATTGTTGTCTTCAACCGCCCTGAGAAACTCACTCTGCCCCCCGCGCCAGTGCCCACGTTTTATCATCATCCGTACGCTCGTTTTCCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTtgctgc                                                                                          >  1:1334267/1‑238 (MQ=255)
                                                                                                                           aCCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTGAATTGTTGTCTTCAACCGCCATGAGAAAATCACTCTGCCCCCAGCGCCAGTGCCAACGGTTTTTCATTATCCGTAAGCCCGTTTACCCCCGGGCCGGACAAAAACTGCTTTTACAATTCAGGGAATAAATCCAGTTTTTCCTGCGtttgtgc                                                                                          >  1:274662/1‑233 (MQ=255)
                                                                                                                             cTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTGAATTGTTTTCTTCAACCCCCCTTAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTTTCATCATCCGTACGCTCGTTTATCGCCCGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGGTGTTCCTGCGTTGCTgccg                                                                                        >  1:1198077/1‑238 (MQ=255)
                                                                                                                             cTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTGAAATGTTGTCTTCAAACCCCATGAGAAAATCAATCTTCCCACAGCGCCAGTGGCAACGTTTTTTTATCATCAGTAACCCCCGTTAACGCCAGGGGGGGCAAAAACTGCTTTAACAATTTAGGAACTAAATCAGGTTGTTCCTGCGTTGCTgccg                                                                                        >  2:617755/1‑238 (MQ=255)
                                                                                                                               aTCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTTAAATGGTGTCTTCAACCGCCCTGAGAAACTCACTCTTCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCCCGTTTACCGCCGGGCTGGGGAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCa                                                                                     >  1:1260394/1‑239 (MQ=255)
                                                                                                                                            agCCGACGAGCAAGGGTAAAAATTAGTTCGAAAATCAGCCTTTATTTTTTTTGTTAAACCGGGCGTTTAAATTGTTGTCTTCAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGaaaaa                                                                        <  1:1021560/239‑1 (MQ=255)
                                                                                                                                                  cgaGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTGAATTGTTGTCTTCAACCGCCATGAGAAACTCACTCTTCCCACAGCGCCAGTGCCAACGTTTTTTCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTTACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCCTTGAAAAACGGGGt                                                                  >  1:494350/1‑239 (MQ=255)
                                                                                                                                                       aaagaaaaaaaTTATTTCGCTAATCAGCCTTTTTATTTATTTTTAAAACTGGGGGTTAAATTTTTTTCTTAAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTaaaga                                                             <  1:1101029/232‑1 (MQ=255)
                                                                                                                                                           agCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTGAATTGTTGTCTTCAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGCGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAgg                                                         >  1:568244/1‑239 (MQ=255)
                                                                                                                                                                   aggTTCGTTACACACCCGTTATTTTATTTGTTAAAACGGGGGGTGAATTGTTGGCTTCAATCCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTGATCATCATCCGTACGCGCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTGGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATgag                                                 <  1:1101241/237‑1 (MQ=255)
                                                                                                                                                                     gTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTGAATTGTTGTCTTCAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCCGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCCGGCTGGACAAAAACTGCTTTAACAATTCCGGTAATAAATCGGGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTTAAAAACGGGGTAAAGAAAAGCACATgaggg                                               >  1:723251/1‑238 (MQ=255)
                                                                                                                                                                           tAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTGAATTTTTTTTTTCAACCGCCATGAGAAAACCACTCTTCCCACAGCGCCCGTGCCAACGTTTTATCTTTATTCCGACGCCCGGTTATCACCGGGGTGGACAAAAAATGCCTTTACAATTCAGGTAATAAATCGGATTGTTTCCTCATCGCTGCCGCCCGTCCATTGAAAAAAGGGGTAAAGCAAGGCACATAAGGGGAATTa                                         >  2:409412/1‑239 (MQ=255)
                                                                                                                                                                                 gCCTTTATATTTTTTTTATACACGGGGGGTTGATTGTTTTTCCTCAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGc                                   <  1:488447/239‑1 (MQ=255)
                                                                                                                                                                                            tattGTTTAAACTGGCGGTTTAATTGTTGTCTTCAACCGCCATGAGAAACTCACTCTGCCCCCAGCGCCCGTGCCAACGTTTTATCATCATCCCTACGCTCGTTTACCCCCCGGCTGGGCAAAAAATTCTTTTACAATTCAGGGAATAAATCGAGTTGTTTCTGCGTTGCCGCCCCCAGGCCATTTAAAAAAGGGGGAAAAAAAAGCACCAGAAGGGGAATATTCAGCATATTTaaag                         >  2:1265149/1‑236 (MQ=255)
                                                                                                                                                                                            tattGTTTAAACTGGCGGTTGAATTGTTGTCTTTAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCCGGGCCAACGTTTTTTCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTTACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCCGTCCATTGAAAAACGGGGGAAAGAAAGGCACATGAGGAGGATTATCCAGCATATTTAACg                         >  1:1478506/1‑238 (MQ=255)
                                                                                                                                                                                               tGTTTAAACTGGCGGTTGAATTTTTTTCTTCAAACGCCATGAGAAACCCACTCTGCCCACAGCGCCAGTGCCAAAGTTTTTTCCTCATTCCTACGCTCGTTTACCCCCCGGCTGGACAAAAAATGCTTTAATAATTCCGGTAATAAAATGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCAATGAAAAAAAGGGTAAAGAAAAGCACATTAGGAGAATTATCCAGGATATTTTACGACaa                     >  2:1177603/1‑238 (MQ=255)
                                                                                                                                                                                               tGTTTAAACTGGCGGTTGAATTGTTGTCTTCAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGGTTTATCATCATCCGGACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTTACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCCTTGAAAAACCGGGGAAAGAAAGGCACATGAGGAGAATTATCCAGCATATTTactttc                       >  1:1369070/1‑232 (MQ=255)
                                                                                                                                                                                                   tAAACTGGCGGTTGAATTGTTGTCTTCAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTTACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGCATATTTAACGCCAGTCg                  >  1:1260263/1‑238 (MQ=255)
                                                                                                                                                                                                        gggCGGTTAAATTGTTTTCTACAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGCATATTTAACGCCAGTCGCTGGAt            <  1:87208/238‑1 (MQ=255)
                                                                                                                                                                                                          gCGGTTGAATTGTTGTCTTCAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCCGTGCCAACGTTTTTTCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGGACATtagga                                               >  1:1372737/1‑202 (MQ=255)
                                                                                                                                                                                                                    ttttttgtTTCCACCCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGCATATTTAACGCCAGTCGCTGGATACCATCCGTa  <  1:818027/235‑1 (MQ=255)
                                                                                                                                                                                                                    aTTGTTGTCTCCAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGCATATTTAACGCCAGTCGCTGGATACCATCCGTa  <  1:321336/239‑1 (MQ=255)
                                                                                                                                                                                                                            |                                                                                                                                                                                                                                         
TATTGTTACAAACGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTATACCATTGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTGAATTGTTGTCTTCAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGCATATTTAACGCCAGTCGCTGGATACCATCCGTA  >  NC_000913/2151475‑2151921

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: