BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	4,256,053	G→C	14.4%	G138G (GGC→GGG)	plsB ←	glycerol‑3‑phosphate O‑acyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,256,053	0	G	C	14.4%	345.4 / 10.4	135	G138G (GGC→GGG)	plsB	glycerol‑3‑phosphate O‑acyltransferase
Reads supporting (aligned to +/- strand): ref base G (43/71); new base C (14/7); total (57/78)
Fisher's exact test for biased strand distribution p-value = 1.68e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 135 total aligned reads displayed.

ATCCTGACGAGCAGGCAAACGTGGGCCTACGGCAGCCAGACGTTGACGGGCAAAGTG‑CATACGCGCCACGCGCGCCAGTTTCTGAGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAG‑CCACA‑GTACAGCGAAAAATTTC‑TGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTT‑CACGCCCCGGCGCGC‑GACCAAACATC‑ACCGACACTGGCACCATCTGCACAT‑CCAGATTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGAACAGCTTAATAGACTCTTCTTTCGGCGTGTAATAGGTGAACACACGCGGCCCGCCGTGAATGAACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGC  >  NC_000913/4255822‑4256283

aTCCTGACGAGCAGGCAAACGTGGGCCTACGGCAGCCAGACGTTGACGGGCAAAGTG‑CATACGCGCCACGCGCGCCAGTTTCTGAGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAG‑CCACA‑GTACAGCGAAAAATTTC‑TGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTTGCCttttt                                                                                                                                                                                                                                     >  1:1506502/1‑239 (MQ=255)

aTCCTGACGAGCAGGCAAACGTGGGCCTACGGCAGCCAGACGTTGACGGGCAAAGTG‑CATACGCGCCACGCGCGCCAGTTTCTGAGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAG‑CCACA‑GTACAGCGAAAAATTTC‑TGTACGCCGTTAAGCATACGCAGCGGCGGGTGCACCTTGCCttttt                                                                                                                                                                                                                                     >  2:329513/1‑239 (MQ=255)

aTCCTGACGAGCAGGCAAACGTGGGCCTACGGCAGCCAGACGTTGACGGGCAAAGTG‑CATACGCGCCACGCGCGCCAGTTTCTGAGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAG‑CCACA‑GTACAGCGAAAAATTTC‑TGTACGCCGTTAAGCATACGCAGCGGCGGGGTTACTTCGCCttttt                                                                                                                                                                                                                                     >  1:1336053/1‑239 (MQ=255)

aTCCTGACGAGCAGGCAAACGTGGGCCTACGGCAGCCAGACGTTGACGGGCAAAGTG‑CATACGCGCCACGCGCGCCAGTTTCTGAGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAG‑CCACA‑GTACAGCGAAAAATTTC‑TGTACGCCGTTAAGCATACGCAGCGGCGGGGTTACCTCGCCttttt                                                                                                                                                                                                                                     >  1:924803/1‑239 (MQ=255)

aTCCTGACGAGCAGGCAAACGTGGGCCTACGGCAGCCAGACGTTGACGGGCAAAGTG‑CATACGCGCCACGCGCGCCAGTTTCTGAGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAG‑CCACA‑GTACAGCGAAAAATTTC‑TGTACGCCGTTAAGCATACGCAGCGGCGGGGTCACTTCGCCctttt                                                                                                                                                                                                                                     >  1:64375/1‑239 (MQ=255)

  ccTGACGAGCAGGCAAACGTGGGCCTACGGCAGCCAGACGTTGACGGGCAAAGTG‑CATACGCGCCACGCGCGCCAGTTTCTGAGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAG‑CCACA‑GTACAGCGAAAAATTTC‑TGTACGCCGTTAAGCATACGCAGCGGCGGGGTCACTTCGCCtttttt                                                                                                                                                                                                                                    >  1:882806/1‑237 (MQ=255)

  ccTGACGAGCAGGCAAACGTGGGCCTACGGCAGCCAGACGTTGACGGGCAAAGTG‑CATACGCGCCACGCGCGCCAGTTTCTGAGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAG‑CCACA‑GTACAGCGAAAAATTTC‑TGTACGCCGTTAAGCATACGCAGCGGCGGGGTCACCTCCCCtttttt                                                                                                                                                                                                                                    >  2:616669/1‑237 (MQ=255)

     gACGAGCAGGCAAACGTGGGCCTACGGCAGCCAGACGTTGACGGGCAAAGTG‑CATACGCGCCACGCGCGCCAGTTTCTGAGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAG‑CCACA‑GTACAGCGAAAAATTTC‑TGTACGCCGTTAAGCATACGCAGCGGCGGGTTTTCCTCGCCTTTTT‑TAcc                                                                                                                                                                                                                                >  1:640906/1‑237 (MQ=255)

       cGAGCAGGCAAACGTGGGCCTACGGCAGCCAGACGTTGACGGGCAAAGTG‑CATACGCGCCACGCGCGCCAGTTTCTGAGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAG‑CCACA‑GTACAGCGAAAAATTTC‑TGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTT‑CACGccc                                                                                                                                                                                                                             <  1:97605/239‑1 (MQ=255)

          gCAGGCAAACGTGGGCCTACGGCAGCCAGACGTTGACGGGCAAAGTG‑CATACGCGCCACGCGCGCCAGTTTCTGAGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAG‑CCACA‑GTACAGCGAAAAATTTC‑TGTACGCCGTTAAGCATACGCAGCGGCGGGTTCCCCTCGCCCTTTT‑CTCGccccc                                                                                                                                                                                                                           >  2:878974/1‑237 (MQ=255)

          gCAGGCAAACGTGGGCCTACGGCAGCCAGACGTTGACGGGCAAAGTG‑CATACGCGCCACGCGCGCCAGTTTCTGAGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAG‑CCACA‑GTACAGCGAAAAATTTC‑TGTACGCCGTTAAGCATACGCAGCGGCGGGGTCACTTCCCCCTTTT‑TAccccccc                                                                                                                                                                                                                           >  2:466687/1‑237 (MQ=255)

           cAGGCAAACGTGGGCCTACGGCAGCCAGACGTTGACGGGCAAAGTG‑CATACGCGCCACGCGCGCCAGTTTCTGAGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAG‑CCACA‑GTACAGCGAAAAATTTC‑TGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTTGCCTTTTT‑CTCGcccccg                                                                                                                                                                                                                          >  1:211670/1‑236 (MQ=255)

           cAGGCAAACGTGGGCCTACGGCAGCCAGACGTTGACGGGCAAAGTG‑CATACGCGCCACGCGCGCCAGTTTCTGAGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAG‑CCACA‑GTACAGCGAAAAATTTC‑TGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCCCCTTTTT‑CACGCCCCCgc                                                                                                                                                                                                                         >  1:487540/1‑239 (MQ=255)

           cAGGCAAACGTGGGCCTACGGCAGCCAGACGTTGACGGGCAAAGTG‑CATACGCGCCACGCGCGCCAGTTTCTGAGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAG‑CCACA‑GTACAGCGAAAAATTTC‑TGTACGCCGTTAAGCATACGCAGCGGCGGGTGTACCTCGCCtttttttagcacccg                                                                                                                                                                                                                           >  2:525736/1‑228 (MQ=255)

             ggCAAACTTGGGCCTACGGAGGCCGGACGTTGACGGGCAAAGTG‑CATACGCGCCACGCGCGCCAGTTTCTGAGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAG‑CCACA‑GTACAGCGAAAAATTTC‑TGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTT‑CACGCCCCGgcg                                                                                                                                                                                                                        <  1:1016983/238‑1 (MQ=255)

               cAAACGGGGGCCTACGGCGGCCAGACGTTGACGGGCAAAGTG‑CCTACGCGCCACGCGCGCCAGTTTCTGAGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAG‑CCACA‑GTACAGCGAAAAATTTC‑TGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTT‑CACGCCCCGgcgcg                                                                                                                                                                                                                      <  2:1207131/238‑1 (MQ=255)

                aaaCGTGGCCCTACGGCAGCCAGACGTTGACGGGCAAAGTG‑CATACGCGCCACGCGCGCCAGTTTCTGAGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAG‑CCACA‑GTACAGCGAAAAATTTC‑TGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTT‑CACGCCCCGgcgcgc                                                                                                                                                                                                                     <  1:567642/238‑1 (MQ=255)

                 aaCGTGGGCCTACGGCAGCCAGGCGTGGACGGGCAAAGTG‑CATGCGCGCCACGCGCGCCAGTTTCTGAGCGATAGTTTTACCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAG‑CCACA‑GTACAGCGAAAAATTTC‑TGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTT‑CACGCCCCGGCGCGC‑Ga                                                                                                                                                                                                                  <  2:1316210/239‑1 (MQ=255)

                     tGGGCCTACGGCAGCCAGACGTTGACGGGCAAAGTG‑CATACGCGCCACGCGCGCCAGTTTCTGAGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAG‑CCACA‑GTACAGCGAAAAATTTC‑TGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCCCCCTTTT‑TACGCCCCCGCGCGC‑GGCCaa                                                                                                                                                                                                              >  1:103141/1‑239 (MQ=255)

                       ggCCTACGGCAGCCAGACGTTGACGGGCAAAGTG‑CATACGCGCCACGCGCGCCAGTTTCTGAGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAG‑CCACA‑GTACAGCGAAAAATTTC‑TGTACGCCGTTAAGCATACGCAGCGGCGGGGTCCCTTCCCCTTTTT‑TACCCCCCCGCCCGC‑GCCCcaa                                                                                                                                                                                                             >  2:1334398/1‑238 (MQ=255)

                        gCCTACGGCAGCCAGACGTTGACGGGCAAAGTG‑CATACGCGCCACGCGCGCCAGTTTCTGAGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAG‑CCACA‑GTACAGCGAAAAATTTC‑TGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTT‑CACGCCCCGGCGCGC‑GACCcaaaa                                                                                                                                                                                                           >  1:1354283/1‑237 (MQ=255)

                        gCCTACGGCAGCCAGACGTTGACGGGCAAAGTG‑CATACGCGCCACGCGCGCCAGTTTCTGAGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAG‑CCACA‑GTACAGCGAAAAATTTC‑TGTACGCCGTTAAGCATACGCAGCGGCGGGGGCCCTTCCCCTTTTT‑TCCGCCCCCGCCCGC‑GCCCCAAc                                                                                                                                                                                                            >  1:69454/1‑238 (MQ=255)

                         ccTACGGCCGCCAGATGTTGACGGGAAAAGTG‑CATCCGCGCCCCGCGCGCCAGTTTCTGAGCGATAGTTTTATCCGTGCCGTGTCCATCCGCCATACGGCGCAGCGAAACTGCCGGGGAGAAACGCACAAAACTGTCGCGACCGAG‑CCACA‑GTACAGCGAAAAATTTC‑TGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTT‑CACGCCCCGGCGCGC‑GACCAAACa                                                                                                                                                                                                           <  1:890021/238‑1 (MQ=255)

                             cGGCAGCCAGACGTTGACGGGCAAAGTG‑CATACGCGCCACGCGCGCCAGTTTCTGAGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCCGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAG‑CCACA‑GTACAGCGAAAAATTTC‑TGTACGCCGTTAAGCATACGCAGCGGCGGGTTTCCTTCCCCCCTTT‑CCTGCCCCGGCCCCC‑CCCCAAAcacc‑ac                                                                                                                                                                                                      >  2:1514048/1‑238 (MQ=255)

                               gCAGCCAGACGTTGACGGGCAAAGTG‑CATACGCGCCACGCGCGCCAGTTTCTGAGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAG‑CCACA‑GTACAGCGAAAAATTTC‑TGTACGCCGTTAAGCATACGCAGCGGCGGGGTCACTTCGCCTTTTT‑TACCCCCCGGCGCGC‑GCCCAAAAATC‑ACCg                                                                                                                                                                                                    >  1:326162/1‑238 (MQ=255)

                                agcccAGACGTTGCCGGGCAAAGTG‑CATACGCGCCCCGCGCGCCAGTTTCTGAGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAG‑CCACA‑GTACAGCGAAAAATTTC‑TGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTT‑CACGCCCCGGCGCGC‑GACCAAACATC‑ACCGa                                                                                                                                                                                                   <  2:663636/235‑1 (MQ=255)

                                 acccaagcGTTGGCGGGAAAAGGT‑AATACGCGCCCCGCGCCCAGGTTTCTGAGCAATAGTTTTCCCCTTCCCGTGTTCGCCCGCAAAAGGGCGCAGCGAAACGGACGGCGAAAAACGCAAAAAACTGTCGCGCCCGAG‑CCACA‑GTACAGCGAAAAATTTC‑TGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTT‑CACGCCCCGGCGCGC‑GACCAAACATC‑ACCGaca                                                                                                                                                                                                 <  2:191499/232‑1 (MQ=255)

                                  gCCAGCCGTGGACGGGCAAAGTG‑CATCCGCGCCACGCGCGCCAGTTTCTGAGCGATAGTTTTACCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAG‑CCACA‑GTACAGCGAAAAATTTC‑TGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTT‑CACGCCCCGGCGCGC‑GACCAAACATC‑ACCGaca                                                                                                                                                                                                 <  2:211670/238‑1 (MQ=255)

                                  gCCAGACGTTGACGGGCAAAGTG‑CATACGCGCCACGCGCGCCAGTTTCTGAGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAG‑CCACA‑GTACAGCGAAAAATTTC‑TGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTT‑TACCCCCCCGCGCGC‑GACCCAACATC‑Accccca                                                                                                                                                                                                 >  1:340067/1‑234 (MQ=255)

                                   ccAGACGTTGACGGGCAAAGTG‑CATACGCGCCACGCGCGCCAGTTTCTGAGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAG‑CCACA‑GTACAGCGAAAAATTTC‑TGTACGCCGTTAAGCATACGCAGCGGCGGGGTCACTTCGACTTTTT‑TACCCCCCGGCTCGC‑GACCCAAAAAT‑ACCGacac                                                                                                                                                                                                >  1:121036/1‑238 (MQ=255)

                                    cAGCCGTTG‑CGGGGCAAGTGCCATCCGCGCCCCGCGCGCCAGTTTCTGACCGATAGTTTTAGCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGCCCGAG‑CCACA‑GTACAGCGAAAAATTTC‑TGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTT‑CACGCCCCGGCGCGC‑GACCAAACATC‑ACCGaca                                                                                                                                                                                                 <  2:326162/236‑1 (MQ=255)

                                    cAGACGTTGACGGGCAAAGTG‑CATCCGCGCCCCGCGCGCCAGTTTCTGAGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAG‑CCACA‑GTACAGCGAAAAATTTC‑TGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTT‑CACGCCCCGGCGCGC‑GACCAAACATC‑ACCGaca                                                                                                                                                                                                 <  1:329513/236‑1 (MQ=255)

                                       ccGTTGACGGGCAAAGGC‑CATACGCCCCACGCGCGCCAGTTCCGGGGCAATAGTTTTACCCGTGCGGTGTTCTCCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAG‑CCACA‑GTACAGCGAAAAATTTC‑TGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTT‑CACGCCCCGGCGCGC‑GACCAAACATC‑ACCGACACTGGc                                                                                                                                                                                            <  2:882806/237‑1 (MQ=255)

                                       aCGTTGACGGGCAAAGTG‑CATACGCGCCACGCGCGCCAGTTTCTGAGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAG‑CCACA‑GTACAGCGAAAAATTTC‑TGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTTGCCTTTTT‑TCCCCCCCGGCGCGC‑GACCACAAATA‑Acccgccacggc                                                                                                                                                                                            >  1:413979/1‑229 (MQ=255)

                                         tgtGACGGGAAAAGTG‑CATACGCGCCCCGCGCGCCAGTTTCTGAGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAG‑CCACA‑GTACAGCGAAAAATTTC‑TGTACGCCTTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTT‑CACGCCCCGGCGCGC‑GACCAAACATC‑ACCGACACTGGCAcc                                                                                                                                                                                         <  1:525736/237‑1 (MQ=255)

                                             aCGGGCAAAGTG‑CATACGCGCCACGCGCGCCAGTTTCTGAGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAG‑CCACA‑GTACAGCGAAAAATTTC‑TGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACCTCGCCTTTTT‑TACCCCCCGGCGCGCGGCCCAAACA‑C‑ACCGACACCGGCAccc                                                                                                                                                                                        >  2:149660/1‑235 (MQ=255)

                                               gggCAACGTG‑CATACGCGCCACGCGCGCCAGTTTCTGAGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAG‑CCACA‑GTACAGCGAAAAATTTC‑TGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTT‑CACGCCCCGGCGCGC‑GACCAAACATC‑ACCGACACTGGCACCATCTGc                                                                                                                                                                                   <  1:1376778/239‑1 (MQ=255)

                                               ggcgaaaGTG‑CACACCCGCCACGCGCCCCAGTTCCGGAGGGTTGGTTTTATCCGTGCGATGTCCCGCCGCCATACGGCGCTGCGAACCTGACGGCGAGAAACACACAAAACTGTCGCGGCCGAG‑CCACA‑GTACAGCGAAAAATTTC‑TGTCCGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTT‑CACGCCCCGGCGCGC‑GACCAAACATC‑ACCGACACTGGCACCATCTg                                                                                                                                                                                    <  2:538490/234‑1 (MQ=255)

                                                 gCAAAGTG‑CATACGCGCCACGCGCGCCAGTTTCTGAGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAG‑CCACA‑GTACAGCGAAAAATTTC‑TGTACGCCGTTAAGCATACGCAGCGGCGGGGTCACTTCGCCTTTTT‑TACGCCCCGCCGCGC‑GACCAAACATC‑ACCGACACCGGACCCACCTGccc                                                                                                                                                                                 >  1:1120687/1‑237 (MQ=255)

                                                           aTACGCGCCACGCGCGCCAGTTTCTGAGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACGGACGGCGAGAAACGCACAAAACTGTCGCGACCGAG‑CCACA‑GTACAGCGAAAAATTTC‑TGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTT‑CACGCCCCGGCGCGC‑GACCAAACATC‑ACCGACACTGGCACCATCTGCACAT‑CCAGAt                                                                                                                                                                        <  1:705008/238‑1 (MQ=255)

                                                            tACGCGCCACGCGCGCCAGTTTCTGAGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAG‑CCACA‑GTACAGCGAAAAATTTC‑TGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTT‑CACGCCCCGGCGCCC‑CACCAAACATC‑CCCGGCACTGGGACCCTCTGCCCCA‑CCCGAttt                                                                                                                                                                      >  1:1260624/1‑239 (MQ=255)

                                                            tACGCGCCACGCGCGCCAGTTTCTGAGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAG‑CCACA‑GTACAGCGAAAAATTTC‑TGTACGCCGTTAAGCATACGCAGCGGCGGGGTTACTTCGCCTTTTT‑CACGCCCCCGCCCCC‑GCCCAAACCTC‑ACCCCCCCTGGCACCCTCTTCCCCT‑CCCGAttt                                                                                                                                                                      >  1:135470/1‑239 (MQ=255)

                                                                cgcCACGCGCGCCAGTTTCTGAGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAG‑CCACA‑GTACAGCGAAAAATTTC‑TGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCCTTTT‑CACCCCCCGGCCGGC‑GACCAAACAAC‑ACCCACACCAGCTACCCCTGCACAC‑CCCGATTTgggg                                                                                                                                                                  >  2:341639/1‑238 (MQ=255)

                                                                  ccACGCGCGCCAGTTTCTGAGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAG‑CCACA‑GTACAGCGAAAAATTTC‑TGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTT‑TACGCCCCCGCCCGC‑GACCAAACATC‑ACCCGCACTGGCACCCCCTGCCCAT‑CCACATTTTGGtt                                                                                                                                                                 >  1:978033/1‑238 (MQ=255)

                                                                  ccACGCGCGCCAGTTTCTGAGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAG‑CCACA‑GTACAGCGAAAAATTTC‑TGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCCCCTTTTT‑TACGCCCCGGCGCGC‑GACCCAACAAC‑ACCGCCACTGGGCCCATCTGCACCT‑CCCAGTTTTGGTTg                                                                                                                                                                >  1:817642/1‑239 (MQ=255)

                                                                      gcgcgcCAGTTTCTGAGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAG‑CCACA‑GTACAGCGAAAAATTTC‑TGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTT‑CACGCCCCGGCGCGC‑GACCAAACATC‑ACCGACACTGGCACCATCTGCACAT‑CCAGATTTGGGTTGCTAc                                                                                                                                                            <  1:466687/239‑1 (MQ=255)

                                                                          gcCAGTTTTTGAGCGATGTGTTTATCCGTGCCGTGTTCACCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAG‑CCACA‑GTACAGCGAAAAATTTC‑TGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTT‑CACGCCCCGGCGCGC‑GACCAAACATC‑ACCGACACTGGCACCATCTGCACAT‑CCAGATTTGGGTTGCTACGGTg                                                                                                                                                        <  2:487540/239‑1 (MQ=255)

                                                                           ccAGTTTCTGAGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAG‑CCACA‑GTACAGCGAAAAATTTC‑TGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTT‑CACGCCCCGGCGCGC‑CACCAAAAACC‑ACCCACACTGGCCCCATCTGCACCT‑CCAGATTTGGGTTTCTACGGTGc                                                                                                                                                       >  1:688769/1‑239 (MQ=255)

                                                                                      ggaaTAGTTTTATCCGTGCCGGGTGCACCCGCAAAACGGCGAAGCGAAACGGACGGCGAGAAACGCACAAAACTTTCGCGACCGAG‑CCACA‑GTACAGCGAAAAATTTC‑TGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTT‑CACGCCCCGGCGCGC‑GACCAAACATC‑ACCGACACTGGCACCATCTGCACAT‑CCAGATTTGGGTTGCTACGGTGCAAATCGAGAt                                                                                                                                             <  2:924803/235‑1 (MQ=255)

                                                                                      gCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAG‑CCACA‑GTACAGCGAAAAATTTC‑TGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTT‑CACGCCCCGGCGCGC‑GACCAAACATC‑ACCGACACTGGCACCATCTGCACAT‑CCAGATTTGGGTTGCTACGGTGCAAATCGAGAt                                                                                                                                             <  1:562415/238‑1 (MQ=255)

                                                                                      gCGATAGTTTTATCCGTGCCGTGTTCACCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAG‑CCACA‑GTACAGCGAAAAATTTC‑TGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTT‑CACGCCCCGGCGCGC‑GACCAAACATC‑ACCGACACTGGCACCATCTGCACAT‑CCAGATTTGGGTTGCTACGGTGCAAATCGAGAt                                                                                                                                             <  1:1514048/238‑1 (MQ=255)

                                                                                      cggATAGTGTTAGCCGGGCCGTTTTCATCCACCATAGGGCGCAGGGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAG‑CCACA‑GTACAGCGAAAAATTTC‑TGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTT‑CACGCCCCGGCGCGC‑GACCAAACATC‑ACCGACACTGGCACCATCTGCACAT‑CCAGATTTGGGTTGCTACGGTGCAAATCGAGAt                                                                                                                                             <  2:826538/236‑1 (MQ=255)

                                                                                         aTAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAG‑CCACA‑GTACAGCGAAAAATTTC‑TGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTT‑TACTCCCCCGCCCGC‑GCACAAACAACAACCAACACT‑GCACCCTCTGCCCATCCCAATTTTGGGTTCTTCGGGTGCAAAGC‑AGATAGTc                                                                                                                                         >  2:663356/1‑239 (MQ=255)

                                                                                         aTAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAG‑CCACA‑GTACAGCGAAAAATTTC‑TGTACGCCGTTAAGCATACGCAGCGGCGGGGTCACCTCGCCTTTTT‑TACGCCCCGGCGCGC‑GACCCAACATC‑ACCGACACTGGCACCATCTTCACCT‑CCCGATTTGGGTTGGTACGGTGCAAATCGAGATAGTc                                                                                                                                         >  1:422738/1‑239 (MQ=255)

                                                                                              tgaacccgtgccgtgTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAG‑CCACA‑GTACAGCGAAAAATTTC‑TGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTT‑CACGCCCCGGCGCGC‑GACCAAACATC‑ACCGACACTGGCACCATCTGCACAT‑CCAGATTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTgg                                                                                                                                     <  2:69454/233‑1 (MQ=255)

                                                                                               ttATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAG‑CCACA‑GTACAGCGAAAAATTTC‑TGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTT‑CACGCCCCGGCGCGC‑GACCAAACATC‑ACCGACACTGGCACCATCTGCACAT‑CCAGATTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGaa                                                                                                                                   <  1:1189943/239‑1 (MQ=255)

                                                                                               ttATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAG‑CCACA‑GTACAGCGAAAAATTTC‑TGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTT‑CACGCCCCGGCGCGC‑GACCAAACATC‑ACCGACACTGGCACCATCTGCACAT‑CCAGATTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGaa                                                                                                                                   <  1:1067615/239‑1 (MQ=255)

                                                                                               ttATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAG‑CCACA‑GTACAGCGAAAAATTTC‑TGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTT‑CACGCCCCGGCGCGC‑GACCAAACATC‑ACCGACACTGGCACCATCTGCACAT‑CCAGATTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGaa                                                                                                                                   <  1:498482/239‑1 (MQ=255)

                                                                                               ttACCCGGGCCGGGTCCATCCGGCATACGGCGCAGCGAAACTGACGGCGAAAAACGCACAAAACTGTCGCGACCGGG‑CCACA‑GTACAGCGAAAAATTTC‑TGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTT‑CACGCCCCGGCGCGC‑GACCAAACATC‑ACCGACACTGGCACCATCTGCACAT‑CCAGATTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGaa                                                                                                                                   <  2:1161246/239‑1 (MQ=255)

                                                                                               ttACCCGGGCCGGGGCCACCCGCCATACGGCGCAGCGAAAATGACGGCGAAAAACGCACAAAACTGCCGCGACCGGG‑CCACA‑GTACAGCGAAAAATTTC‑TGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTT‑CACGCCCCGGCGCGC‑GACCAAACATC‑ACCGACACTGGCACCATCTGCACAT‑CCAGATTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGaa                                                                                                                                   <  2:413979/239‑1 (MQ=255)

                                                                                               ttAACCGTGCCGGCTCCAGCCGCCATGCGGCGCAGCGAAACGGACGGCGAGAAACCCAAAAACCTGTCGCGCCCGAG‑CCACA‑GTACAGCGAAAATTTTC‑GTTACGCCGTTAAGCATACGCGGCGGCGGGTTCACTTCGCCTTTTT‑CACGCCCCGGCGCGC‑GACCAAACATC‑ACCGACACTGGCACCATCTGCACAT‑CCAGATTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGaa                                                                                                                                   <  2:970535/239‑1 (MQ=255)

                                                                                               tgaTCCGGGACGTGTGCACCCACCATACGGCACAGCGAAACTGACGGCGAGAAACGGAAAAAGCGGTCTCGACCGAG‑CCACA‑GTCCAGCGAAAAATTTC‑TGTACGCCAGTAAGCAGACGCAGCGGCGGGTTCAGTTGGCCTTTTT‑CCCGCCCCGGCGCGC‑GGCCAACCATC‑CCCGACGCTGGCACCAGCTGCACAT‑CCAGATTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGaa                                                                                                                                   <  2:1088057/237‑1 (MQ=255)

                                                                                               taaccggtgccgggTTAACCCGCCATACGGCGCAGCGAAACGTACGGCGAGAAACGCACAAAACTGTCGCGACCGAG‑CCGCA‑GTACAGCGAAAAATTTC‑TGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTT‑CACGCCCCGGCGCGC‑GACCAAACATC‑ACCGACACTGGCACCATCTGCACAT‑CCAGATTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGaa                                                                                                                                   <  2:789170/233‑1 (MQ=255)

                                                                                                gagcagcgacgtgGTCGTCCGCCATGCGGCGCGACGAAACTGGCGGCGAGAAACGCGCAACACTGGGGGGACCGGG‑CCGAA‑GTACAGCGAAAAATTTC‑TGGACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCATTTTT‑CACGCCCCGGCGCGC‑GACCAAACATC‑ACCGACACTGGCACCATCGGCACAT‑CCAGATTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGaa                                                                                                                                   <  2:34262/229‑1 (MQ=255)

                                                                                                      tgccgtgTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAG‑CCACA‑GTACAGCGAAAAATTTC‑TGTACGCCGTTAAGCATACGCAGCGGCGGGGTCACTTCGCCTTTTT‑TTCCCCCCCGCGCGC‑CACCAAACATC‑ACCCACACTCGCACCATCTCCACAC‑CCAGATTTTGGTTGCTTCGGTGCCAATCAAGATAGACGTGGGACAGGttt                                                                                                                            >  2:572923/1‑238 (MQ=255)

                                                                                                       gccgtgTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAG‑CCACA‑GTACAGCGAAAAATTTC‑TGTACGCCGTTAAGCATACGCAGCGGCGGGGTCCCTTCGCCTTTTT‑TACCCCCCGGCGCGC‑GGCCCAACACC‑CCCCACCCCGGCACCCTCTCCCCCT‑CCCGATTTTGGGTGCTACGGGGGAAATCGAGAAAGGCGTGGGACAGCATaa                                                                                                                           >  2:868270/1‑239 (MQ=255)

                                                                                                            gtTCATCCGCCATACGGCGCAGCAAAACTGACGGCGAGAAACGCACAAAACGGCCGCGACCGAG‑CCACA‑GTACAGCGAAAAATTTC‑TGTACGCCGTTAAGCATACGCAGGGGCGGGTTCACTTCGCCTTTTG‑CACGCCCCGGCGCGC‑GACCAAACATC‑ACCGACACTGGCACCATCTGCACAT‑CCAGATTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGAACAGCTTAATAGAc                                                                                                                      <  1:1093365/239‑1 (MQ=255)

                                                                                                            gtTCACCCGCCATACGGCGCAGCGAAACGGACGGCGAAAAACGCACAAAACTGTCGCGACCGAG‑CCACA‑GTACAGCGAAAAATTTC‑TGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTT‑CACGCCCCGGCGCGC‑GACCAAACATC‑ACCGACACTGGCACCATCTGCACAT‑CCAGATTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGAACAGCTTAATAGAc                                                                                                                      <  1:700338/239‑1 (MQ=255)

                                                                                                             ttCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAG‑CCACA‑GTACAGCGAAAAATTTC‑TGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTT‑CACGCCCCGGCGCGC‑GACCAAACATC‑ACCGACACTGGCACCATCTGCACAT‑CCAGATTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGAACAGCTTAATAGAc                                                                                                                      <  1:885972/238‑1 (MQ=255)

                                                                                                             tgctaccGCCAAGCGGGGCAGCGAAACTGTGGGGGAGAAACCAACAAAACGGTGGCGACCGAG‑CCACA‑GTACAGCGAAAAATTTC‑TGTACGCCGTTAAGCATGCGCAGCGGCGGGTTCACTTCGCCTTTTT‑CACGCCCCGGCGCGC‑GACCAAACATC‑GCCGACACTGGCACCATCTGCACAT‑CCAGATTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGAACAGCTTAATAGAct                                                                                                                     <  2:1406699/234‑1 (MQ=255)

                                                                                                                aTCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAG‑CCACA‑GTACAGCGAAAAATTTC‑TGTACGCCGTTAAGCATACGCAGCGGCGGGGTCCCTTCCCCTTTTT‑TACGCCCCCGCGCGC‑GACCCAACATC‑ACCCACACCGGCACCCTCTCCACAT‑CCCCATTTGGGGAGGTAACGTGCGAATAGCGATAGTCGGTGAACAACTTAAATAACtctt                                                                                                                  >  2:1410055/1‑239 (MQ=255)

                                                                                                                  ccGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAG‑CCACA‑GTACAGCGAAAAATTTC‑TGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTTGCCTTTTT‑TACGCCCCCGCGCGC‑GACCCAACATC‑ACCGGCACCGGCACCCTCTTCCCAT‑CCCCATTTTGGGTGCTACGGGGCCAATCGAGATAGTCGGGGAACAGCTTAAAAAACtcctct                                                                                                                >  1:278446/1‑239 (MQ=255)

                                                                                                                    gCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAG‑CCACA‑GTACAGCGAAAAATTTC‑TGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTT‑CACGCCCCGGCGCGC‑GACCAAACATC‑ACCGACACTGGCACCATCTGCACAT‑CCAGATTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGAACAGCTTAATAGACTCTTCttt                                                                                                              <  1:991722/239‑1 (MQ=255)

                                                                                                                     ccATACTGCGCAGCGAAACTGACAGCGAGAAACGCACAAAACAGTCGCTACCGAG‑CCACA‑GTACAGCGAAAACTTTC‑TGTACGCCGTTAAGCATACGCAGCGGCGGGATTCCTTTGCCGCTTT‑TTCGGCCCCGCTCGC‑GGCCCTACATCAACCGACCCTTCCA‑CACCTCTACAC‑CCCGACTTGGATTGCTTCGGTGCCAATCGAGATAACCGCTGACACGTTTAATAGACCCTTCttt                                                                                                              >  2:5728/1‑238 (MQ=255)

                                                                                                                       aTACGGCGCAGCGAAGCTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAG‑CCACA‑GTACAGCGAAAAATTTC‑TGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTT‑CACGCCCCGGCGCGC‑GACCAAACATC‑ACCGACACTGGCACCATCTGCACAT‑CCAGATTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGAACAGCTTAATAGACTCTTCTTTCgg                                                                                                           <  1:412119/239‑1 (MQ=255)

                                                                                                                       aTACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAG‑CCACA‑GTACAGCGAAAAATTTC‑TGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTT‑CACGCCCCGGCGCGC‑GACCAAACATC‑ACCGACACTGGCACCATCTGCACAT‑CCAGATTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGAACAGCTTAATAGACTCTTCTTTCgg                                                                                                           <  1:1479587/239‑1 (MQ=255)

                                                                                                                       aTACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAG‑CCACA‑GTACAGCGAAAAATTTC‑TGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTT‑CACGCCCCGGCGCGC‑GACCAAACATC‑ACCGACACTGGCACCATCTGCACAT‑CCAGATTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGAACAGCTTAATAGACTCTTCTTTCgg                                                                                                           <  1:244740/239‑1 (MQ=255)

                                                                                                                       aTACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAG‑CCACA‑GTACAGCGAAAAATTTC‑TGTACGCCGTTAAGCATACGCAGCGGCGGGGTCACTTCGCCCTTTT‑TTCCCCCCGGCGCGC‑GGCCAAACATC‑ACCCACCCTGGCACCCACCTCCCAC‑CCAGATTTTGGTTGCTTCCGGGCAAATCGAGATAGTGGTGGGACAGCTTAATAAACACTTCTTTTgg                                                                                                           >  2:1479587/1‑239 (MQ=255)

                                                                                                                       aTACGGCGCAGCGAAACTGACGGCGAGAAACGCAAAAAACTGTCGCGACCGAG‑CCACA‑GTACAGCGAAAAATTTC‑TGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTT‑CACGCCCCGGCGCGC‑GACCAAACATC‑ACCGACACTGGCACCATCTGCACAT‑CCAGATTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGAACAGCTTAATAGACTCTTCTTTCgg                                                                                                           <  1:1195483/239‑1 (MQ=255)

                                                                                                                         aCGGCGCAGCGAAACTGACGGCGAGAAACGCAAAAAACGGTCGCGACCGAG‑CCACA‑GTACAGCGAAAAATTTC‑TGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTT‑CACGCCCCGGCGCGC‑GACCAAACATC‑ACCGACACTGGCACCATCTGCACAT‑CCAGATTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGAACAGCTTAATAGACTCTTCTTTCGGCg                                                                                                         <  1:482940/239‑1 (MQ=255)

                                                                                                                             cgcAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAG‑CCACA‑GTACAGCGAAAAATTTC‑TGTACGCCGTTAAGCATACGCAGCGGCGGGGTCACCTCGCCTTTTT‑CACCCCCCCGCCCGC‑GACCAAAAAAC‑AACCGCACCGGCCCCCCCCTCACAA‑CCACACTTGGGGTGCTTCGGTGCAAACCCAGATAATCGTGGGACCGCTTAATAGCATCTTTTTTCTGCGTGTa                                                                                                     >  2:1382704/1‑239 (MQ=255)

                                                                                                                              gcACCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGCCCGAG‑CCACA‑GTACAGCGAAAAATTTC‑TGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTT‑CACGCCCCGGCGCGC‑GACCAAACATC‑ACCGACACTGGCACCATCTGCACAT‑CCAGATTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGAACAGCTTAATAGACTCTTCTTCCGGCGTGTaa                                                                                                    <  1:878974/239‑1 (MQ=255)

                                                                                                                                aGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAG‑CCACA‑GTACAGCGAAAAATTTC‑TGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTT‑TACGCCCCGGCGCGC‑GACCAAACATC‑ACCGACACTGGCACCATCTGCACAT‑CCCGATTTTGGTTGCTACGGGGCAAATCGAGATAGTCGTGGAACAGCTTAATAAACTCTTCTTTTGGCGTGTAATa                                                                                                  >  1:1432040/1‑239 (MQ=255)

                                                                                                                                  cGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAG‑CCACA‑GTACAGCGAAAAATTTC‑TGTACGCCGTTAAGCATACGCAGCGGCGGGTTTACTTCGCCTTTTT‑TACGCCCCCGCGCGC‑GACCCAACATC‑ACCGACACTGGCACCCTCTGCACAT‑CCAGATTTTGGGTGCTACGGTGCAAATCGAGATAGACGGGGAACAGCTTAATAGACTCTTCTTTCGGCGTGTGATAgg                                                                                                >  1:426682/1‑239 (MQ=255)

                                                                                                                                   gAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAG‑CCACA‑GTACAGCGAAAAATTTC‑TGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTT‑CACGCCCCGGCGCGC‑GACCAAACATC‑ACCGACACTGGCACCATCTGCACAT‑CCAGATTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGAACAGCTTAATAGACTCTTCTTTCGGCGTGTAATAgg                                                                                                <  1:1286181/238‑1 (MQ=255)

                                                                                                                                   gAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAG‑CCACA‑GTACAGCGAAAAATTTC‑TGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTT‑CACGCCCCGGCGCGC‑GACCAAACATC‑ACCGACACTGGCACCATCTGCACAT‑CCAGATTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGAACAGCTTAATAGACTCTTCTTTCGGCGTGTAATAgg                                                                                                <  1:773432/238‑1 (MQ=255)

                                                                                                                                                     gcaaaaaacCTGTCGCGCCCG‑G‑CCCCAGGTACA‑CCAAAAATTTCTTGTCCGCCGTAAGGCATCCGCGGGGGGGGTTTCACTCCGCTTTTTT‑CCCCCCCCGGCGCGC‑GACAAAACCCC‑ACCGACACTGGCACCATCTGCACAT‑CCAGATTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGAAACAGCTTAATAGACTCTTCTTGCGGCGTGTAATAGGTGAACACACGCGGCccgcc                                                                             <  2:1120687/234‑1 (MQ=255)

                                                                                                                                                     cccaaaaaaCCGTCGCGCCCGGGCCCGCA‑GTCCAGCAAAAATTTTG‑TGTCCCCGGTAAAGCACCCGCCGCGGCGGTTCCACTTCCCCTTTTC‑CCCCCCCGGGCGCGC‑ACCAAAACACC‑CCCGACACGGGCACCATCTGAACAT‑CCAGATTGGGGTTGCTACGGTGAAAATCGAGATAGGCGGGAAACAGCTTAATAGACTCTTCTTTCGGCGTGTAATGGGTGAACACACGCGGCccgc                                                                              <  2:817642/234‑1 (MQ=255)

                                                                                                                                                            aaCTGTCGCGACCGAG‑CCACA‑GTACAGCGAAAAATTTC‑TGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTT‑CACGCCCCGGCGCGC‑GACCAAACATC‑ACCGACACTGGCACCATCTGCACAt                                                                                                                                                                               <  1:639408/135‑1 (MQ=255)

                                                                                                                                                                     gACCGAG‑CCAAA‑GTACAGCAAAAATTTTC‑TGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCTTTTTT‑CCCGCCCCGGCGCGC‑GACCAAACACC‑CCCGACACTGGCACCATCTGCACAT‑CAAGATTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGAACAGCTTAATAGACTCTTCTTTCGGCGTGTAATAGGTGAACACCCGCGGCCCGCCGTGAATGAACACATAg                                                             <  1:1248105/239‑1 (MQ=255)

                                                                                                                                                                      gccGAG‑CCACA‑GTACAGAAAAAATTTTT‑TGTCCCCGGTTAAGCATCGGCGGGGGGGGTTTCCCTTCCCTTTTTC‑CCCGCCCCGGCGCGC‑GAAAAAACATC‑CCCGACCCGGGCACCATCTGCACAT‑CAAGATTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGAACAGCTTAATAGACTCTTCTTTCGGCGTGTAATAGGTGAACCCACGCGGCCCGCCGTGAATGAACACATAg                                                             <  2:1331567/237‑1 (MQ=255)

                                                                                                                                                                      aCCGAG‑CCACA‑GTACAGCGAAAAATTTC‑TGTACGCCGTTAAGCATACGCAGCGGCGGGGTCACTTTGCCTTTTT‑CACGCCCCCGCCCGC‑GACCAAAAATC‑ACCGACACCGGCACAATATGCACAA‑CCAGCATTGGGTTGCTCCGGGGCAAAACGAGATAGTGGTGGGACAGCTTAATAGAGCCCTCTTTCGGCGGGTAAAATGTGAACAAACCCGGCCCCGCGCGGATGAAACCATAg                                                             >  2:175532/1‑238 (MQ=255)

                                                                                                                                                                        cGAG‑CCACA‑GTACAGCGAAAAATTTC‑TGTACGCCGTTAAGCATACGCAGCGGCGGGTTAACTTTCCCTTTTT‑CACGCCCCCGCGCGC‑GACCAAAAATC‑AACGCGCCCGGCACCATCTGCACCT‑CCAGATTTAGGTTGCTACTGTGCAAAAACAGATAGTGGGTGGACAACTTAATCAACTCTTTGTTAGGCGAGTAATAGGAGAAGCAACACGGGCTGCCGTGAAGAAACAAATAacgg                                                          >  2:520836/1‑238 (MQ=255)

                                                                                                                                                                                       aGCGAAAAATTTC‑TGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTT‑CACGCCCCGGCGCGC‑GACCAAACATC‑ACCGACACTGGCACCCTCTGCACAT‑CCAGATTTTGGTTGCTACGGTGCAAATCGAGATAGTCGTGGAACAGCTTAATAGACTCTTCTTTCGGCGGGTAATAGGTGAACACACCCGGCCCGCCGCGAATGAACACATAGCGCGGCAGTAGCggg                                              >  1:537349/1‑236 (MQ=255)

                                                                                                                                                                                       aGCGAAAAATTTC‑TGTACGCCGTTAAGCATACGCAGCGGCGGGGTCACTTCGCCTTTTT‑CACGCCCCGGCGCGC‑GACCAAACATC‑ACCGACACTGGCACCATCTGCACAT‑CCAGATTTGGGGTGCTAATGTGCAAAACGATATATTCTTCTATTCGCGTTATAGACTCTTCTTTCGGCGGGTAATAGGGGAACACACGCGGCCCGCCGTGAATGAACACATAGCGCGGCAGGAGCGTg                                              >  1:900844/1‑238 (MQ=255)

                                                                                                                                                                                         cGAAAAATTTC‑TGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTTCCCTTTTT‑TACGCCCCCGCGCGC‑GGCCCAACATC‑ACCCACACCGGCACCCTCTTCACCT‑CCCGATTTTGGGTGCTACGGTGCAAAACGAGGAAGTCGTGGGACAGCTTAATAAACTCTTCTTTTGGCGGGTAATAAGGGAACACACCCGGCCCCCCGGGGATGCACACCTAACGCGGCAGAAGGGCGcc                                            >  2:24404/1‑238 (MQ=255)

                                                                                                                                                                                                     tGTCCGCCGTTAAGCATACGCAGCGGCGGGTTCATTTCCCCTTTTT‑CCCGCCCCGGCGCGC‑GACCAAACATC‑ACCGACACGGGCACCATCTGCACAT‑CCAGATTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGAACAGCTTAATAGACTCTTCTTTCGGCGTGTAATAGGTGAACACACGCGGCCCGCCGTGAATGAACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAg                                <  1:501511/239‑1 (MQ=255)

                                                                                                                                                                                                                   cATACGCAGCGGCGGGTTCACTTCGCCTTTTT‑CACGCCCCGGCGCGC‑GACCAAACATC‑ACCGACACTGGCACCATCTGCACAT‑CCAGATTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGAACAGCTTAATAGACTCTTCttt                                                                                                              <  1:1042940/147‑1 (MQ=255)

                                                                                                                                                                                                                    atccGCAGCGGCGGGTTCCCTTCCCCTTTTC‑CACCCCCCGGCGCGC‑AACAAAACATC‑CCCGACACTGGCCCCATCTGCACAT‑CCAAATTGGGGTTGCTACGGTGAAAATCGAGATAGTCGTGGAACAGCTTAATAGACTCTTCTTTCGGCGTGTAATAGGTGAACACCCGCGGCCCGCCGTGAATGAACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGt                  <  1:35781/235‑1 (MQ=255)

                                                                                                                                                                                                                    aacccccgcggcgGGTTCCCCTGCCCTTTTTCCCCCCCCCGGCGCGC‑GCCAAAACACC‑CCCGACACTGGCCCCATCTGCACAT‑CAAGATTGGGGTGGCTCGGGTGAAAATCGAGATAGTCGTGAAACAGCTAAATAGACTCTTCTTTCGGGGTTTAATAGGTGACCACCCGCGGCCCGCCGTGAATGAACATATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGt                  <  2:1217878/232‑1 (MQ=255)

ATCCTGACGAGCAGGCAAACGTGGGCCTACGGCAGCCAGACGTTGACGGGCAAAGTG‑CATACGCGCCACGCGCGCCAGTTTCTGAGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAG‑CCACA‑GTACAGCGAAAAATTTC‑TGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTT‑CACGCCCCGGCGCGC‑GACCAAACATC‑ACCGACACTGGCACCATCTGCACAT‑CCAGATTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGAACAGCTTAATAGACTCTTCTTTCGGCGTGTAATAGGTGAACACACGCGGCCCGCCGTGAATGAACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGC  >  NC_000913/4255822‑4256283

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`