Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,285,877 C→G 13.3% P134A (CCG→GCG)  agaD → N‑acetylgalactosamine‑specific enzyme IID component of PTS

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,285,8770CG13.3% 262.9 / 6.4 106P134A (CCG→GCG) agaDN‑acetylgalactosamine‑specific enzyme IID component of PTS
Reads supporting (aligned to +/- strand):  ref base C (21/69);  new base G (14/2);  total (35/71)
Fisher's exact test for biased strand distribution p-value = 1.63e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 114 total aligned reads displayed.

CGCGGCGATGAAAGATAACCTCGAATTTATTAATACCCACCCGAATCTGGTCGGATTCCTGATGGGGTTATTAATTTCGATGGAAGAAAAAGGAGAAAACCGCGACACCATTAAAGGCCTCAAAGTGGCACTGTTTGGCCCAATCGCCGGGATTGGCGATGCGATTTTCTGGTTTACTTTGTTGCCGATTATGGC‑GGGAATTTGCTCA‑TCATTTGCCAGCCAG‑GGAAACCTGCT‑GGGGCCGATTCTA‑TTTTTCGCCGTTTACCTGCTTATCTTTTTCCTGCGCGTCGGC‑TGGACCCACGTCGGTTATTCAGTCGGCGTGAAGGCGATCGATAAAGTGCGAGAGAACTCGCAGATGATTGCCCGTTCGGCAACCATCCTCGGGATCACGGTAATCGGCGGGCTGATCGCTTCGTATGTGCATATTAACGTGGTGACATCGTTTGCCATCGACAATACCCACAGCGTTGCGCTGCAGCA  >  NC_000913/3285639‑3286115
                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                
cgcgGCGAAGAAAGATAACGTCGAATTTATTAATACCCACCCGAATCTGGTCGGATTCCTGATGGAGTTATTAATTTCGATGGAAGAAAAAGGAGAAAACCGCGACACCATTAAAGGCCTCAAAGTGGCACTGTTTGGCCCAATCGCCGGGATTGGCGATGCGATTTTCTGGTTTACTTTGTTGCCGATTATGGC‑GGGAATTTGCTCA‑TCTTTTGCCAGCCAG‑GGAAACCTGCT‑GGGGc                                                                                                                                                                                                                                                  <  1:1032758/239‑1 (MQ=255)
 gcggcgATGAAAGATAACCTCGAATTTATTAATACCCACCCGAATCTGGTCGGATTCCTGATGGGGTTATTAATTTCGATGGAAGAAAAAGGAGAAAACCGCGACACCATTAAAGGCCTCAAAGTGGCACTGTTTGGCCCAATCGCCGGGATTGGCGATGCGATTTTCTGGTTTACTTTGTTGCCGATTATGGC‑GGGAATTTGCTCA‑TCATTTGCCAGCCAG‑GGAAACCTGCT‑GGGGc                                                                                                                                                                                                                                                  <  1:460701/238‑1 (MQ=255)
 gcggcgATGAAAGATAACCTCGAATTTATTAATACCCACCCGAATCTGGTCGGATTCCTGATGGGGTTATTAATTTCGATGGAAGAAAAAGGAGAAAACCGCGACACCATTAAAGGCCTCAAAGTGGCACTGTTTGGCCCAATCGCCGGGATTGGCGATGCGATTTTCTGGTTTACTTTGTTGCCGATTATGGC‑GGGAATTTGCTCA‑TCATTTGCCAGCCAG‑GGAAACCTGCT‑GGGGc                                                                                                                                                                                                                                                  <  1:505734/238‑1 (MQ=255)
 gcggcgATGAAAGATAACCTCGAATTTATTAATACCCACCCGAATCTGGTCGGATTCCTGATGGGGTTATTAATTTCGATGGAAGAAAAAGGAGAAAACCGCGACACCATTAAAGGCCTCAAAGTGGCACTGTTTGGCCCAATCGCCGGGATTGGCGATGCGATTTTCTGGTTTACTTTGTTGCCGATTATGGC‑GGGAATTTGCTCA‑TCATTTGCCAGCCAG‑GGAAACCTGCT‑GGGGc                                                                                                                                                                                                                                                  <  2:623222/238‑1 (MQ=255)
   ggagATGAAAGATAACCTCGAATTTATTAATACCCACCCGAATCTGGTCGGATTCCTGATGGGGTTATTAATTTCGATGGAAGAAAAAGGAGAAAACCGCGACTCCATTAAAGGCCTCAAAGTGGCACTGTTTGGCCCAATCGCCGGGATTGGCGATGCGATTTTCTGGTTTACTTTGTTGCCGATTATGGC‑GGGAATTTGCTCA‑TCATTTGCCAGCCAG‑GGAAACCTGCT‑GGGGCCGa                                                                                                                                                                                                                                               <  1:1507958/236‑1 (MQ=255)
               tagacTCGATTTTATTAATACCCGCCCGAATCTGGTCGGATTCCTGATGGGGTTATTAATTTCGATGGAAGAAAAAGGAGAAAACCGCGACACCATTAAAGGCCTCAAAGTGGCACTGTTTGGCCCAATCGCCGGGATTGGCGATGCGATTTTCTGGTTTACTTTGTTGCCGATTATGGC‑GGGAATTTGCTCA‑TCATTTGCCAGCCAG‑GGAAACCTGCT‑GGGGCCGATTCTA‑TTTTTCg                                                                                                                                                                                                                                  <  2:46691/235‑1 (MQ=255)
               tAACCTCGAATTTATTAATACCCACCCGAATCTGGTCGGATTCCTGATGGGGTTATTAATTTCGATGGAAGAAAAAGGAGAAAACCGCGACACCATTAAAGGCCTCAAAGTGGCACTGTTTGGCCCAATCGCCGGGATTGGCGATGCGATTTTCTGGTTTACTTTGTTGCCGATTATGGC‑GGGAATTTGCTCA‑TCATTTGCCAGCCAG‑GGAAACCTGCT‑GGGGCCGATTCTA‑ttttt                                                                                                                                                                                                                                    <  1:755208/237‑1 (MQ=255)
               tAACCTCGAATTTATTAATACCCACCCGAATCTGGTCGGATTCCTGATGGGGTTATTAATTTCGATGGAAGAAAAAGGAGAAAACCGCGACACCATTAAAGGCCTCAAAGTGGCACTGTTTGGCCCAATCGCCGGGATTGGCGATGCGATTTTCTGGTTTACTTTGTTGCCGATTATGGC‑GGGAATTTGCTCA‑TCATTTGCCAGCCAG‑GGAAACCTGCT‑GGGGCCGATTCTA‑TTTTTCg                                                                                                                                                                                                                                  <  1:1316818/239‑1 (MQ=255)
                aaCCGCGAATTTATGAATTCCCACGCGAATGTGGTCGGATTCCTGATGGGGTTATTAATTTCGATGGAAGAAAAAGGAGGAAACCGCGACACCATTAAAGGGCTCAAAGTGGCACGGGTTGGCCCAATCGCCGGGATTGGCGATGCGATTTTCTGGTTTACTTTGTTGCCGATTATGGC‑GGGAATTTGCTCA‑TCCTTTGCCAGCCAG‑GGAAACCTGCT‑GGGGCGGATTCTA‑TTTTTCGc                                                                                                                                                                                                                                 <  2:33708/239‑1 (MQ=255)
                 aCCTCGAATTTATTAATACCCACCCGAATCTGGTCGGATTCCTGATGGGGTTATTAATTTCGATGGAAGAAAAAGGAGAAAACCGCGACACCATTAAAGGCCTCAAAGTGGCACTGTTTGGCCCAATCGCCGGGATTGGCGATGCGATTTTCTGGTTTACTTTGTTGCCGATTATGGC‑GGGGATTTTCTCT‑TCCTTTTTCACCCAG‑GGGAACCTCAT‑CGGGCCGCATCTC‑ttttttcc                                                                                                                                                                                                                                 >  1:1313223/1‑235 (MQ=255)
                      gAATTTATTAATACCCACCCGAATCTGGTCGGATTCCTGATGGGGTTATTAATTTCGATGGAAGAAAAAGGAGAAAACCGCGACACCATTAAAGGCCTCAAAGTGGCACTGTTTGGCCCAATCGCCGGGATTGGCGATGCGATTTTCTGGTTTACTTTGTTGCCGATTATGGC‑GGGAATTTGCTCA‑TCATTTGCCAGCCAG‑GGAAACCTGCT‑GGGGCCGATTCTA‑TTTTTCGCCGTTTa                                                                                                                                                                                                                           <  1:209225/239‑1 (MQ=255)
                       aaTTTATTAATACCCACCCGAATCTGGTCGGATTCCTGATGGGGTTATTAATTTCGATGGAAGAAAAAGGAGAAAACCGCGACACCATTAAAGGCCTCAAAGTGGCACTGTTTGGCCCAATCGCCGGGATTGGCGATGCGATTTTCTGGTTTACTTTGTTGCCGATTATGGC‑GGGGATTTTCTCT‑TCTCTTGCCACCCCG‑GGGAACCTGCT‑CGGGGCGGTTCTA‑TTTTTCTcctcttt                                                                                                                                                                                                                           >  2:13292/1‑233 (MQ=255)
                        aTTTATTAATACCCACCCGAATCTGGTCGGATTCCTGATGGGGTTATTAATTTCGATGGAAGAAAAAGGAGAAAACCGCGACACCATTAAAGGCCTCAAAGTGGCACTGTTTGGCCCAATCGCCGGGATTGGCGATGCGATTTTCTGGTTTACTTTGTTGCCGATTATGGC‑GGGAATTTGCTCA‑TCATTTGCCAGCCAG‑GGAAACCTGCT‑GGGGCCGATTCTA‑TTTTTCGCCGTTTAc                                                                                                                                                                                                                          <  2:1120939/238‑1 (MQ=255)
                        aTTTATTAATACCCACCCGAATCTGGTCGGATTCCTGATGGGGTTATTAATTTCGATGGAAGAAAAAGGAGAAAACCGCGACACCATTAAAGGCCTCAAAGTGGCACTGTTTGGCCCAATCGCCGGGATTGGCGATGCGATTTTCTGGTTTACTTTGTTGCCGATTATGGC‑GGGAATTTGCTCA‑TCATTTGCCAGCCAG‑GGAAACCTGCT‑GGGGCCGATTCTA‑TTTTTCGCCGTTTAc                                                                                                                                                                                                                          <  2:1588052/238‑1 (MQ=255)
                            attaATACCCACCCGAATCTGGTCGGATTCCTGATGGGGTTATTAATTTCGATGGAAGAAAAAGGAGAAAACCGCGACACCATTAAAGGCCTCAAAGTGGCACTGTTTGGCCCAATCGCCGGGATTGGCGATGCGATTTTCTGGTTTACTTTGTTGCCGATTATGGC‑GGGAATTTGCTCA‑TCATTTGCCAGCCAG‑GGAAACCTGCT‑GGGGCCGATTCTA‑TTTTTCGCCGTTTACCTGCt                                                                                                                                                                                                                     <  1:352037/239‑1 (MQ=255)
                                     cacccGAATCTGGTCGGATTCCTGATGGGGTTATTAATTTCGATCGAAGAAAAAGGAGAAAACCGCGACACCATTAAAGGCCTCAAAGTGGCACTGTTTGGCCCAATCGCCGGGATTGGCGATGCGATTTTCTGGTTTACTTTGTTGCCGATTATGGC‑GGGGAATTGGTTA‑TCATTTGCCAGCCAC‑GGGAAACTGCT‑GGGGGCGAGTTTT‑TTTTTTTCCGGTTTCCTGCTTTTCttttt                                                                                                                                                                                                            >  1:1184264/1‑239 (MQ=255)
                                      acccGAATCTGGTCGGATTCCTGATGGGGTTATTAATTTCGATGGAAGAAAAAGGAGAAAACCGCGACACCATTAAAGGCCTCAAAGTGGCACTGTTTGGCCCAATCGCCGGGATTGGCGATGCGATTTTCTGGTTTACTTTGTTGCCGATTATGGCGGGGATTTTGGTC‑‑TCCTTTTTCAACCCG‑GGGAAACTGCT‑CGGGCCGGTTCTATTTTTTTCCCGTTTACCT‑CTTATCcttttt                                                                                                                                                                                                           >  2:141383/1‑238 (MQ=255)
                                      acccGAATCTGGTCGGATTCCTGATGGGGTTATTAATTTCGATGGAAGAAAAAGGAGAAAACCGCGACACCATTAAAGGCCTCAAAGTGGCACTGTTTGGCCCAATCGCCGGGATTGGCGATGCGATTTTCTGGTTTACTTTGTTGCCGATTATGGC‑GGGGATTTTCTCT‑TTATTTTTGCGCCCG‑GGCGATCTGCT‑GGGGGCGGGTCTT‑TTTTTTGCCTTTTACCTGCTTTTCtttttt                                                                                                                                                                                                           >  2:1606685/1‑238 (MQ=255)
                                      acccGAATCTGGTCGGATTCCTGATGGGGTTATTAATTTCGATGGAAGAAAAAGGAGAAAACCGCGACACCATTAAAGGCCTCAAAGTGGCACTGTTTGGCCCAATCGCCGGGATTGGCGATGCGATTTTCTGGTTTACTTTGTTGCCGATTATGGC‑GGGGATTTGCTCT‑TCATTTTTCTGCCCG‑GGGAAACTGCT‑GGGGCCGGTTTTA‑TTTTTTTTCGTTTTCCTGCttttttttttt                                                                                                                                                                                                           >  1:18826/1‑238 (MQ=255)
                                      acccGAATCTGGTCGGATTCCTGATGGGGTTATTAATTTCGATGGAAGAAAAAGGAGAAAACCGCGACACCATTAAAGGCCTCAAAGTGGCACTGTTTGGCCCAATCGCCGGGATTGGCGATGCGATTTTCTGGTTTACTTTGTTGCCGATTATGGC‑GGGAATTTTCTCA‑TCATTTTCCAGCCAGTGGGAACC‑CAT‑GGGGGCGGTTTTA‑TTTTTTGTCGTTTTCCTGCttttttttttt                                                                                                                                                                                                           >  1:1549682/1‑238 (MQ=255)
                                      acccGAATCTGGTCGGATTCCTGATGGGGTTATTAATTTCGATGGAAGAAAAAGGAGAAAACCGCGACACCATTAAAGGCCTCAAAGTGGCACTGTTTGGCCCAATCGCCGGGATTGGCGATGCGATTTTCTGGTTTACTTTGTTGCCGATTATGGC‑GGGAATTTGCTCT‑TCCTTTGCCAGCCAG‑GGAAACCTGCT‑GGGGGCGGTTCTA‑TTTTTTTCCTTTTACCTGCTTATCtttttt                                                                                                                                                                                                           >  2:915920/1‑238 (MQ=255)
                                      acccGAATCTGGTCGGATTCCTGATGGGGTTATTAATTTCGATGGAAGAAAAAGGAGAAAACCGCGACACCATTAAAGGCCTCAAAGTGGCACTGTTTGGCCCAATCGCCGGGATTGGCGATGCGATTTTCTGGTTTACTTTGTTGCCGATTATGGC‑GGGAATTTGCTCT‑TCATTTTTCACCCCC‑GGCAACCTCCT‑GGGGCGGGTTCTC‑TTTTTTTCCGTTTTTCTGCCTttttttttt                                                                                                                                                                                                           >  2:1532272/1‑238 (MQ=255)
                                      acccGAATCTGGTCGGATTCCTGATGGGGTTATTAATTTCGATGGAAGAAAAAGGAGAAAACCGCGACACCATTAAAGGCCTCAAAGTGGCACTGTTTGGCCCAATCGCCGGGATTGGCGATGCGATTTTCTGGTTTACTTTGTTGCCGATTATGGC‑GGGAAGTTTGTCA‑TCTTTTTCCAGCCCA‑GGACAACTCCT‑GTGGGCGGCTTTC‑TTTTTCTCCCTTTGCCTGTTTACtttttt                                                                                                                                                                                                            >  1:1171155/1‑238 (MQ=255)
                                      acccGAATCTGGTCGGATTCCTGATGGGGTTATTAATTTCGATGGAAGAAAAAGGAGAAAACCGCGACACCATTAAAGGCCTCAAAGTGGCACTGTTTGGCCCAATCGCCGGGATTGGCGATGCGATTTTCTGGTTTACTTTGTTGCCGATTATGGC‑GGGAAATTTCTCA‑TCTATTTCCACTCCC‑GGAAACCCGCC‑GGGGGCCATTCTC‑TTTTTTTCCGTTTTTCTGTTTTTCtttttt                                                                                                                                                                                                           >  2:1312528/1‑238 (MQ=255)
                                       cccGAATCTGGTCGGATTCCTGATGGGGTTATTAATTTCGATGGAAGAAAAAGGAGAAAACCGCGACACCATTAAAGGCCTCAAAGTGGCACTGTTTGGCCCAATCGCCGGGATTGGCGATGCGATTTTCTGGTTTACTTTGTTGCCGATTATGGC‑GGGAATTTTGTCT‑TCCTTTTCCCGCCAG‑GGACACCTGCT‑GGGGCCGGTTCTT‑TTTTTTTCCGTTTACCTGCtttttttttt                                                                                                                                                                                                            >  1:244235/1‑237 (MQ=255)
                                       cccGAATCTGGTCGGATTCCTGATGGGGTTATTAATTTCGATGGAAGAAAAAGGAGAAAACCGCGACACCATTAAAGGCCTCAAAGTGGCACTGTTTGGCCCAATCGCCGGGATTGGCGATGCGATTTTCTGGTTTACTTTGTTGCCGATTATGGC‑GGGAATTTGCTCA‑TCATTTTCCCGCCAG‑GGGAACCTGCT‑GGGGCCGGTTCTA‑TTTTTTTCCCGTTTCCTTCtttttttttt                                                                                                                                                                                                            >  1:443584/1‑237 (MQ=255)
                                                        tCCTGATGGGGTTATTAATTTCGATGGAAGAAAAAGGAGAAAACCGCGACACCATTAAAGGCCTCAAAGTGGCACTGTTTGGCCCAATCGCCGGGATTGGCGATGCGATTTTCTGGTTTACTTTGTTGCCGATTATGGC‑GGGAATTTGCTCA‑TCATTTGCCAGCCAG‑GGAAACCTGCT‑GGGGCCGATTCTA‑TTTTTCGCCGTTTACCTGCTTATCTTTTTCCTGCGCGTCGGC‑TGGAcc                                                                                                                                                                                        <  1:1049103/239‑1 (MQ=255)
                                                           tGATGTGGTTATTAATTTCGATGGAAGAAAAAGAAGAAAACCGCGACACCATTAAAGGCCTCAAAGTGGCACTGTTTGGCCCAATCGCCGGGATTGGCGATGCGATTTTCTGGTTTACTTTGTTGCCGATTATGGC‑GGGAATTTGCTCA‑TCATTTGCCAGCCAG‑GGAAACCTGCT‑GGGGCCGATTCTA‑TTTTTCGCCGTTTACCTGCTTATCTTTTTCCTGCGCGTCGGC‑TGGACCCAc                                                                                                                                                                                     <  1:265826/239‑1 (MQ=255)
                                                           tGATGGGGTTACTAATTTCGAGGGAAGAAAAAGGAGAAAACCGCGACACCATTAAAGGCCTCAAAGTGGCACTGTTTGGCCCAATCGCCGGGATTGGCGATGCGATTTTCTGGTTTACTTTGTTGCCGATTATGGC‑GGGAATTTGCTCA‑TCATTTGCCAGCCAG‑GGAAACCTGCT‑GGGGCCGATTCTA‑TTTTTCGCCGTTTACCTGCTTATCTTTTTCCTGCGCGTCGGC‑TGGACCCAc                                                                                                                                                                                     <  2:387359/239‑1 (MQ=255)
                                                            gATGGGGTTATTAATTTCGATGGAAGAAAAAGGAGAAAACCGCGACACCATTAAAGGCCTCAAAGTGGCACTGTTTGGCCCAATCGCCGGGATTGGCGATGCGATTTTCTGGTTTACTTTGTTGCCGATTATGGC‑GGGGATTTTTTCA‑TCATTTTTCAGCCCG‑GGGAAACTGCT‑GGGGGCGGTTCTT‑TTTTTTGTCGGTTTACTTCTTATCTTTTTTTTTTGCGTCGGC‑Tgggacccc                                                                                                                                                                                     >  1:4898/1‑232 (MQ=255)
                                                                         aattCGATGGAAGAAAAAGGAGAAAACCGCGACACCATTAAAGGCCTCAAAGTGGCACTGTTTGGCCCAATCGCCGGGATTGGCGATGCGATTTTCTGGTTTACTTTGTTGCCGATTATGGC‑GGGAATTTGCTCA‑TCATTTGCCAGCCAG‑GGAAACCTGCT‑GGGGCCGATTCTA‑TTTTTCGCCGTTTACCTGCTTATCTTTTTCCTGCGCGTCGGC‑TGGACCCACGTCGGTTATTCAGt                                                                                                                                                                       <  1:589995/237‑1 (MQ=255)
                                                                         aTTTCGATGGAAGAAAAAGGAGAAAACCGCGACACCATTAAAGGCCTCAAAGTGGCACTGTTTGGCCCAATCGCCGGGATTGGCGATGCGATTTTCTGGTTTACTTTGTTGCCGATTATGGC‑GGGAATTTGCTCA‑TCATTTGCCAGCCAG‑GGAAACCTGCT‑GGGGCCGATTCTA‑TTTTTCGCCGTTTACCTGCTTATCTTTTTCCTGCGCGTCGGC‑TGGACCCACGTCGGTTATTCAGt                                                                                                                                                                       <  2:1392035/239‑1 (MQ=255)
                                                                          tttCGATGGAAGAAAAAGGAGAAAACCGCGACACCATTAAAGGCCTCAAAGTGGCACTGTTTGGCCCAATCGCCGGGATTGGCGATGCGATTTTCTGGTTTACTTTGTTGCCGATTATGGC‑GGGAATTTGCTCA‑TCATTTGCCAGCCAG‑GGAAACCTGCT‑GGGGCCGATTCTA‑TTTTTCGCCGTTTACCTGCTTATCTTTTTCCTGCGCGTCGGC‑TGGACCCACGTCGGTTATTCAGTc                                                                                                                                                                      <  2:750345/239‑1 (MQ=255)
                                                                           ttCGATGGAAGAAAAAGGAGAAAACCGCGACACCATTAAAGGCCTCAAAGTGGCACTGTTTGGCCCAATCGCCGGGATTGGCGATGCGATTTTCTGGTTTACTTTGTTGCCGATTATGGC‑GGGAATTTGCTCA‑TCATTTTTCCGCCCC‑GGAAAACTGCC‑GGGGCCGCTTCTA‑TTTTTTTCCGCTTACCTTCTTATCTTTTTTTTCCGCGGCGGC‑CGGGCCCACGTCGGGTATTTTGTCg                                                                                                                                                                     >  1:251380/1‑239 (MQ=255)
                                                                                  gaagaaAAAGGAGAAACCCGCAACACCTTTAAAGGCCGCAAAGTGGCACTGTTTGGCCCAATCGCCGGGATGGGCGATGCGGTTTTCTGGTTTACTTTGTTGCCGAGTATGGC‑GGGAATTTGCTCA‑TCATTTGCCAGCCAG‑GGAAACCTGCT‑GGGGGCGATTCTA‑TTTTTCGCCGTTTACCTGCTTATCTTTTTCCTGCGCGTCGGC‑TGGACCCCCGTCGGTTATTCAGTCGGCGTGa                                                                                                                                                               <  2:1182759/238‑1 (MQ=255)
                                                                                              gaAAACCGCGACACCATTAAAGGCCTCAAAGTGGCACTGTTTGGCCCAATCGCCGGGATTGGCGATGCGATTTTCTGGTTTACTTTGTTGCCGATTATGGC‑GGGAATTTGCTCA‑TCATTTGCCAGCCAG‑GGAAACCTGCT‑GGGGCCGATTCTA‑TTTTTCGCCGTTTACCTGCTTATCTTTTTCCTGCGCGTCGGC‑TGGACCCACGTCGGTTATTCAGTCGGCGTGAAGGCGATCGATa                                                                                                                                                   <  1:1477975/238‑1 (MQ=255)
                                                                                                         cacCATTAAAGGCCTCAAAGTGGCACTGTTTGGCCCAATCGCCGGGATTGGCGATGCGATTTTCTGGTTTACTTTGTTGCCGATTATGGC‑GGGAATTTGCTCA‑TCATTTGCCAGCCAG‑GGAAACCTGCT‑GGGGCCGATTCTA‑TTTTTCGCCGTTTACCTGCTTATCTTTTTCCTGCGCGTCGGC‑TGGACCCACGTCGGTTATTCAGTCGGCGTGAAGGCGATCGATAAAGTGCgagaga                                                                                                                                       <  1:1055763/239‑1 (MQ=255)
                                                                                                            cATTAAAGGCCTCAAAGTGGCACTGTTTGGCCCAATCGCCGGGATTGGCGATGCGATTTTCTGGTTTACTTTGTTGCCGATTATGGC‑GGGAATTTGCTCA‑TCATTTGCCAGCCAG‑GGAAACCTGCT‑GGGGc                                                                                                                                                                                                                                                  <  1:1625071/131‑1 (MQ=255)
                                                                                                            cATTAAAGGCCTCAAAGTGGCACTGTTTGGCCCAATCGCCGGGATTGGCGATGCGATTTTCTGGTTTACTTTGTTGCCGATTATGGC‑GGGAATTTGCTCA‑TCATTTGCCAGCCAG‑GGAAACCTGCT‑GGGGCCGATTCTA‑TTTTTCGCCGTTTACCTGCTTATCTTTTTCCTGCGCGTCGGC‑TGGACCCACGTCGGTTATTCAGTCGGCGTGAAGGcga                                                                                                                                                         <  1:230774/218‑1 (MQ=255)
                                                                                                                 aaGGCCTCAAAGTGGCACTGTTTGGCCCAATCGCCGGGATTGGCGATGCGATTTTCTGGTTTACTTTGTTGCCGATTATGGC‑GGGGAATTTCTCT‑TCTCTTTTCACCCCC‑GGCGAACCACC‑GGGGGCGGTTTTA‑TTTTTTTCCCGTTACCTGCTTATTTTTTTTTTTCGCGCTGGC‑GGGACCCCCGCCGGGTATTTCTTCGGGCTGGAGGCGGTCGGTAAAGAGCGGGAGAAcactcac                                                                                                                               >  2:1092407/1‑233 (MQ=255)
                                                                                                                 aaGGCCTCAAAGTGGCACTGTTTGGCCCAATCGCCGGGATTGGCGATGCGATTTTCTGGTTTACTTTGTTGCCGATTATGGC‑GGGAATTTGCTCA‑TCATTTGCCAGCCAG‑GGAAACCTGCT‑GGGGCCGATTCTA‑TTTTTCGCCGTTTACCTGCTTATCTTTTTCCTGCGCGTCGGC‑TGGACCCACGTCGGTTATTCAGTCGGCGTGAAGGCGATCGATAAAGTGCGAGAGAACTCGCAg                                                                                                                               <  1:1405658/239‑1 (MQ=255)
                                                                                                                   ggCCTCAAAGTGGCACTGTTTGGCCCAATCGCCGGGATTGGCGATGCGATTTTCTGGTTTACTTTGTTGCCGATTATGGC‑GGGAATTTGCTCT‑TCCTTTTTCAGCCCC‑GGGAACCTGCT‑GGGGGCGATTCTT‑TTTTTTGTCGCTTTCCTGCTTTTTTTTTTTCTTCGCGTCGGC‑TGTGCCCCCGCCGGTTTTTCCGTCGGCGTGAGGGCGGTCGAGAAAAGGCGGGGGAACGCCCAgat                                                                                                                             >  2:424836/1‑239 (MQ=255)
                                                                                                                      cTCAAAGTGGCACTGTTTGGCCCAATCGCCGGGATTGGCGATGCGATTTTCTGGTTTACTTTGTTGCCGATTATGGC‑GGGGATTTGTTCATTCATTTTCC‑GCCCC‑GGGAACCCGCC‑GGGGGCGGTTCTT‑TTTTTTTTCCGTTACCTTCTTACTTTTTTTCTTCGCCTCCGC‑TGGGCCCCCCACGGGTTTTTCGTCGGCGTGCAGGGGGTCGGGTAAGTGGGGGAGAGATCGCCgagt                                                                                                                            >  2:288079/1‑235 (MQ=255)
                                                                                                                      cTCAAAGTGGCACTGTTTGGCCCAATCGCCGGGATTGGCGATGCGATTTTCTGGTTTACTTTGTTGCCGATTATGGC‑GGGAATTTTTTCA‑TCCTTTTTCATCCCG‑GGGAACCCGCT‑GGGGGCGGTTTTA‑TTTTTTTCCGTTTTCCTTCTTTTCTTTTTTCTGCGCGTCGGCTTGGACCC‑CCTCGGTTTTTTTATCGGCGTGTAGGCGTGCGTTCAAATGCGGGAGAAcatggcgcgtgt                                                                                                                          >  2:1674525/1‑228 (MQ=255)
                                                                                                                       tCAAAGTGGCACTGTTTGGCCCAATCGCCGGGATTGGCGATGCGATTTTCTGGTTTACTTTGTTGCCGATTATGGC‑GGGGATTTTCTCC‑TCCTTTTCCAGCCAG‑GGGAACCTGCT‑GGGGCCGGTTCTA‑TTTTTTTCCGTTTTCCTGCTTATCTTTTTTCTTCGCGTCGGC‑CGGGCCCCCGTCGGGTATTTTGTCGGCGCGCAGGCGATCGATAAAGAGGGGGAGAACTCGCAGATGAtt                                                                                                                         >  1:532732/1‑239 (MQ=255)
                                                                                                                          aaGTGGCACTGTTTGGCCCAATCGCCGGGATTGGCGATGCGATTTTCTGGTTTACTTTGTTGCCGATTATGGC‑GGGAATTTGCTCA‑TCATTTGCCAGCCAG‑GGAAACCTGCT‑GGGGCCGATTCTA‑TTTTTCGCCGTTTACCTGCTTATCTTTTTCCTGCGCGTCGGC‑TGGACCCACGTCGGTTATTCAGTCGGCGTGAAGGCGATCGATAAAGTGCGAGAGAACTCGCAGATGATTGcc                                                                                                                      <  2:880579/239‑1 (MQ=255)
                                                                                                                          aaGTGGCACTGTTTGGACCAATCGCCGGGATTGGCGATGCTATTTTCTGGTTTTCGTTGTTGCCTATTATGGC‑GGGAATTTGCTCA‑TCGTTTGCCAGCCAG‑GGAAACCTGCT‑GGGGCCGATTCTA‑TTTTTCGCCGTTTACCTGCTTATCTTTTTCCTGCGCGTCGGC‑TGGACCCACGTCGGTTATTCAGTCGGCGTGAAGGCGATCGATAAAGTGCGAGAGAACTCGCAGATGATTGcc                                                                                                                      <  1:765152/239‑1 (MQ=255)
                                                                                                                              ggCACTGTTTGGCCCAATCGCCGGGATTGGCGATGCGATTTTCTGGTTTACTTTGTTGCCGATTATGGC‑GGGAATTTGCTCA‑TCATTTGCCAGCCAG‑GGAAACCTGCT‑GGGGCCGATTCTA‑TTTTTCGCCGTTTACCTGCTTATCTTTTTCCTGCGCGTCGGC‑TGGACCCACGTCGGTTATTCAGTCGGCGTGAAGGCGATCGATAAAGTGCGAGAGAACTCGCAGATGATTGCCCGtt                                                                                                                  <  1:103176/239‑1 (MQ=255)
                                                                                                                                    gTTTGGCCCAATCGCCGGGATTGGCGATGCGATTTTCTGGTTTACTTTGTTGCCGATTATGGC‑GGGAATTTGCTCA‑TCATTTGCCAGCCAG‑GGAAACCTGCT‑GGGGCCGATTCTA‑TTTTTCGCCGTTTACCTGCTTATCTTTTTCCTGCGCGTCGGC‑TGGACCCACGTCGGTTATTCAGTCGGCGTGAAGGCGATCGATAAAGTGCGAGAGAACTCGCAGATGATTGCCCGTTCGGCaa                                                                                                            <  1:1348574/239‑1 (MQ=255)
                                                                                                                                             aaTCGCCGGGATTGGCGATGCGATTTTCTGGTTTACTTTGTTGCCGATTATGGC‑GGGGAGTTTTTCC‑TCATTTTTCAGCCCG‑GGGAAACTGCTGGGGGCGGGTTTTT‑TTTTTTCCCGTTTCCCT‑CTTTTCTTTTTTCTTCGCGGCGGC‑TGGGCCCCCCTCGGGTTTTTAGTCGGCGGGAAGGGGGTCGGTAAAAGGCGAGGGAACTCGCCGGTTAGTTCCCGGTCGGCCACCATccccc                                                                                                   >  2:1139414/1‑236 (MQ=255)
                                                                                                                                               tCGCCGGGATTGGCGATGCGATTTTCTGGTTTACTTTGTTGCCGATTATGGC‑GGGGAATTGTTCA‑TCCTTTTTCACCCCG‑GGGAACCCGCC‑TGGGGCGAGTCTA‑TTTTTTTCCGTTTTCCTGCTTTTTTTTTTTCTCCGCGTCGGC‑TGGTCCCACGCCGGTTTTTTAGTTCGCGGGAAGGCGGTCGTTAAAGAGCGGGAGAACTACCAGCTGCTTGCCCCTTCGGGCACCATCCTCgg                                                                                                  >  2:265481/1‑238 (MQ=255)
                                                                                                                                                       aTTGGCGATGCGATTTTCTGGTTTACTTTGTTGCCGATTATGGC‑GGGAATTTGCTCA‑TCATTTGCCAGCCAG‑GGAAACCTGCT‑GGGGCCGATTCTA‑TTTTTCGCCGTTTACCTGCTTATCTTTTTCCTGcgcg                                                                                                                                                                                                    <  1:957555/133‑1 (MQ=255)
                                                                                                                                                           gcgatgcgatTTTCTGGTTTACTTTGTTGCCGATTATGGC‑GGGAATTTGCTCA‑TCATTTGCCAGCCAG‑GGAAACCTGCT‑GGGGCCGATTCTA‑TTTTTCGCCGTTTACCTGCTTATCTTTTTCCTGCGCGTCGGC‑TGGACCCACGTCGGTTATTCAGt                                                                                                                                                                       <  1:828962/157‑1 (MQ=255)
                                                                                                                                                                    ttttCTGGTTTACTTTGTTGCCGATTATGGC‑GGGAATTTGCTCA‑TCATTTGCCAGCCAG‑GGAAACCTGCT‑GGGGCCGATTCTA‑TTTTTCGCCGTTTACCTGCTTATCTTTTTCCTGCGCGTCGGC‑TGGACCCACGTCGGTTATTCAGTCGGCGTGAAGGCGATCGATAAAGTGCGAGAGAACTCGCAGATGATTGCCCGTTCGGCAACCATCCTCGGGATCACGGTAATCGGCGGGCTg                                                                            <  1:1716005/239‑1 (MQ=255)
                                                                                                                                                                      ttttGGTTTACTTTGTTGCCGATTATGGC‑GGGAATTTGCTCA‑TCATTTGCCAGCCAG‑GGAAACCTGCT‑GGGGCCGATTCTA‑TTTTTCGCCGTTTACCTGCTTATCTTTTTCCTGCGCGTCGGC‑TGGACCCACGTCGGTTATTCAGTCGGCGTGAAGGCGATCGATAAAGTGCGAGAGAACTCGCAGATGATTGCCCGTTCGGCAACCATCCTCGGGATCACGGTAATCGGCGGGCTGAt                                                                          <  1:1002362/236‑1 (MQ=255)
                                                                                                                                                                      ttCTGGTTTACTTTGTTGCCGATTATGGC‑GGGAATTTGCTCA‑TCATTTGCCAGCCAG‑GGAAACCTGCT‑GGGGCCGATTCTA‑TTTTTCGCCGTTTACCTGCTTATCTTTTTCCTGCGCGTCGGC‑TGGACCCACGTCGGTTATTCAGTCGGCGTGAAGGCGATCGATAAAGTGCGAGAGAACTCGCAGATGATTGCCCGTTCGGCAACCATCCTCGGGATCACGGTAATCGGCGGGCTGAt                                                                          <  2:1428111/239‑1 (MQ=255)
                                                                                                                                                                      ttCTGGTTTACTTTGTTGCCGATTATGGC‑GGGAATTTGCTCA‑TCATTTGCCAGCCAG‑GGAAACCTGCT‑GGGGCCGATTCTA‑TTTTTCGCCGTTTACCTGCTTATCTTTTTCCTGCGCGTCGGC‑TGGACCCACGTCGGTTATTCAGTCGGCGTGAAGGCGATCGATAAAGTGCGAGAGAACTCGCAGATGATTGCCCGTTCGGCAACCATCCTCGGGATCACGGTAATCGGCGGGCTGAt                                                                          <  1:915920/239‑1 (MQ=255)
                                                                                                                                                                      ttCTGGTTTACTTTGTTGCCGATTATGGC‑GGGAATTTGCTCA‑TCATTTGCCAGCCAG‑GGAAACCTGCT‑GGGGCCGATTCTA‑TTTTTCGCCGTTTACCTGCTTATCTTTTTCCTGCGCGTCGGC‑TGGACCCACGTCGGTTATTCAGTCGGCGTGAAGGCGATCGATAAAGTGCGAGAGAACTCGCAGATGATTGCCCGTTCGGCAACCATCCTCGGGATCACGGTAATCGGCGGGCTGAt                                                                          <  2:371928/239‑1 (MQ=255)
                                                                                                                                                                      ttCTGGTTTACTTTGTTGCCGATTATGGC‑GGGAATTTGCTCA‑TCATTTGCCAGCCAG‑GGAAACCTGCT‑GGGGCCGATTCTA‑TTTTTCGCCGTTTACCTGCTTATCTTTTTCCTGCGCGTCGGC‑TGGACCCACGTCGGTTATTCAGTCGGCGTGAAGGCGATCGATAAAGTGCGAGAGAACTCGCAGATGATTGCCCGTTCGGCAACCATCCTCGGGATCACGGTAATCGGCGGGCTGAt                                                                          <  1:424836/239‑1 (MQ=255)
                                                                                                                                                                         tGGTTTACTTTGTTGCCGATTATGGC‑GGGAATTTGCTCA‑TCATTTGCCAGCCAG‑GGAAACCTGCT‑GGGGCCGATTCTA‑TTTTTCGCCGTTTACCTGCTTATCTTTTTCCTGCGCGTCGGC‑TGGACCCACGTCGGTTATTCAGTCGGCGTGAAGGCGATCGATAAAGTGCGAGAGAACTCGCAGATGATTGCCCGTTCGGCAACCATCCTCGGGATCACGGTAATCGGCGGGCTGATCGc                                                                       <  1:1397347/239‑1 (MQ=255)
                                                                                                                                                                         tGGTTTACTTTGTTGCCGATTATGGC‑GGGAATTTGCTCA‑TCATTTGCCAGCCAG‑GGAAACCTGCT‑GGGGCCGATTCTA‑TTTTTCGCCGTTTACCTGCTTATCTTTTTCCTGCGCGTCGGC‑TGGACCCACGTCGGTTATTCAGTCGGCGTGAAGGCGATCGATAAAGTGCGAGAGAACTCGCAGATGATTGCCCGTTCGGCAACCATCCTCGGGATCACGGTAATCGGCGGGCTGATCGc                                                                       <  1:681852/239‑1 (MQ=255)
                                                                                                                                                                            tttACTTTGTTGTCGATTATGGC‑GGGAATTTTCTCA‑TCATCTGCCAGCCAG‑GTAAACCTGCT‑GGGGCCGATTCTA‑TTTTTCGCCGTTTACCTGCTTATCTTTTTCCTGCGCGTCGGC‑TGGACCCACGTCGGTTATTCAGTCGGCGTGAAGGCGATCGATAAAGTGCGAGAGAACTCGCAGATGATTGCCCGTTCGGCAACCATCCTCGGGATCACGGTAATCGGCGGGCTGATCGCtt                                                                     <  2:943343/238‑1 (MQ=255)
                                                                                                                                                                            tttACTTTGTTGCCGATTATGGC‑GGGAATTTGCTCA‑TCATTTGCCAGCCAG‑GGAAACCTGCT‑GGGGCCGATTCTA‑TTTTTCGCCGTTTACCTGCTTATCTTTTTCCTGCGCGTCGGC‑TGGACCCACGTCGGTTATTCAGTCGGCGTGAAGGCGATCGATAAAGTGCGAGAGAACTCGCAGATGATTGCCCGTTCGGCAACCATCCTCGGGATCACGGTAATCGGCGGGCTGATCGCtt                                                                     <  1:1587598/238‑1 (MQ=255)
                                                                                                                                                                              tACTTTGTTGCGGATTATGGC‑GGGAATTTGCTCA‑TCATTTGCCAGCCAG‑GGAAACCTGCT‑GGGGCCGATTCTA‑TTTTTCGCCGTTTACCTGCTTATCTTTTTCCTGCGCGTCGGC‑TGGACCCACGTCGGTTATTCAGTCGGCGTGAAGGCGATCGATAAAGTGCGAGAGAACTCGCAGATGATTGCCCGTTCGGCAACCATCCTCGGGATCACGGTAATCGGCGGGCTGATCGCTTCGt                                                                  <  1:538434/239‑1 (MQ=255)
                                                                                                                                                                              tACTTTGTTGCCGATTATGGC‑GGGAATTTGCTCA‑TCATTTGCCAGCCAG‑GGAAACCTGCT‑GGGGCCGATTCTA‑TTTTTCGCCGTTTACCTGCTTATCTTTTTCCTGCGCGTCGGC‑TGGACCCACGTCGGTTATTCAGTCGGCGTGAAGGCGATCGATAAAGTGCGAGAGAACTCGCAGATGATTGCCCGTTCGGCAACCATCCTCGGGATCACGGTAATCGGCGGGCTGATCGCTTCGt                                                                  <  1:502692/239‑1 (MQ=255)
                                                                                                                                                                              tACTTTGTTGCCGATTATGGC‑GGGAATTTGCTCA‑TCATTTGCCAGCCAG‑GGAAACCTGCT‑GGGGCCGATTCTA‑TTTTTCGCCGTTTACCTGCTTATCTTTTTCCTGCGCGTCGGC‑TGGACCCACGTCGGTTATTCAGTCGGCGTGAAGGCGATCGATAAAGTGCGAGAGAACTCGCAGATGATTGCCCGTTCGGCAACCATCCTCGGGATCACGGTAATCGGCGGGCTGATCGCTTCGt                                                                  <  2:244235/239‑1 (MQ=255)
                                                                                                                                                                              tACTTTGTTGCCGATTATGGC‑GGGAATGTGCTCA‑TCATTTGCCAGCCAG‑GGAAACCTGCT‑GGGGCCGATTCTA‑TTTGTCGCCGTTTACCTGCTTATCTTTTTCCTGCGCGTCGGC‑TGGACCCACGGCGGTTATTCAGTCGGCGTGAAGGCGATCGATAAAGTGCGAGAGAACTCGCAGATGATTGCCCGTTCGGCAACCATCCTCGGGATCACGGTAATCGGCGGGCTGATCGCTTCGt                                                                  <  1:1225797/239‑1 (MQ=255)
                                                                                                                                                                                 tttGTTGCCGATTATGGC‑GGGAATTTGCTCA‑TCATTTGCCAGCCAG‑GGAAACCTGCT‑GGGGCCGATTCTA‑TTTTTCGCCGTTTACCTGCTTATCTTTTTCCTGCGCGTCGGC‑TGGACCCACGTCGGTTATTCAGTCGGCGTGAAGGCGATCGATAAAGTGCGAGAGAACTCGCAGATGATTGCCCGTTCGGCAACCATCCTCGGGATCACGGTAATCGGCGGGCTGATCGCTTCGTAtg                                                               <  1:1312528/239‑1 (MQ=255)
                                                                                                                                                                                  ttgttgCCGATTATGGC‑GGGAATTTGCTCA‑TCATTTGCCAGCCAG‑GGAAACCTGCT‑GGGGGCGATTCTA‑TTTTTCGCCGTTTACCTGCTTATCTTTTTCCTGCGCGTCGGC‑TGGACCCACGTCGGTTATTCAGTCGGCGTGAAGGCGATCGATAAAGTGCGAGAGAACTCGCAGATGATTGCCCGTTCGGCAACCATCCTCGGGATCACGGTAATCGGCGGGCTGATCGCTTCGTAtgt                                                              <  1:1432375/239‑1 (MQ=255)
                                                                                                                                                                                  ttgttgCCGATTATGGC‑GGGAATTTGCTCA‑TCATTTGCCAGCCAG‑GGAAACCTGCT‑GGGGCCGATTCTA‑TTTTTCGCCGTTTACCTGCTTATCTTTTTCCTGCGCGTCGGC‑TGGACCCACGTCGGTTATTCAGTCGGCGTGAAGGCGATCGATAAAGTGCGAGAGAACTCGCAGATGATTGCCCGTTCGGCAACCATCCTCGGGATCACGGTAATCGGCGGGCTGATCGCTTCGTAtgt                                                              <  1:923728/239‑1 (MQ=255)
                                                                                                                                                                                  ttgttgCCGATTATGGC‑GGGAATTTGCTCA‑TCATTTGCCAGCCAG‑GGAAACCTGCT‑GGGGCCGATTCTA‑TTTTTCGCCGTTTACCTGCTTATCTTTTTCCTGCGCGTCGGC‑TGGACCCACGTCGGTTATTCAGTCGGCGTGAAGGCGATCGATAAAGTGCGAGAGAACTCGCAGATGATTGCCCGTTCGGCAACCATCCTCGGGATCACGGTAATCGGCGGGCTGATCGCTTCGTAtgt                                                              <  2:14188/239‑1 (MQ=255)
                                                                                                                                                                                   tgttgCCGATTATGGC‑GGGAATTTGCTCA‑TCATTTGCCAGCCAG‑GGAAACCTGCT‑GGGGCCGATTCTA‑TTTTTCGCCGTTTACCTGCTTATCTTTTTCCTGCGCGTCGGC‑TGGACCCACGTCGGTTATTCAGTCGGCGTGAAGGCGATCGATAAAGTGCGAGAGAACTCGCAGATGATTGCCCGTTCGGCAACCATCCTCGGGATCACGGTAATCGGCGGGCTGATCGCTTCGTAtgtg                                                             <  1:265481/239‑1 (MQ=255)
                                                                                                                                                                                   tgttgCCGATTAGGGC‑GGGAATTGGCTCA‑TCATTTGCCAGCCAG‑GGAAACCTGCT‑GGGGCCGATTCTA‑TTTTTCGCCGTTTACCTGCTTATCTTTTTCCTGCGCGTCGGC‑TGGACCCGCGTCGGTTATTCAGTCGGCGTGAAGGCGATCGATAAAGTGCGAGAGAACTCGCAGATGATTGCCCGTTCGGCAACCATCCTCGGGATCACGGTAATCGGCGGGCTGATCGCTTCGTAtgtg                                                             <  2:295689/239‑1 (MQ=255)
                                                                                                                                                                                      tgCCGATTATGGC‑GGGAATTTGCTCA‑TCATTTGCCAGCCAG‑GGAAACCTGCT‑GGGGCCGATTCTA‑TTTTTCGCCGTTTACCTGCTTATCTTTTTCCTGCGCGTCGGC‑TGGACCCACGTCGGTTATTCAGTCGGCGTGAAGGCGATCGATAAAGTGCGAGAGAACTCGCAGATGATTGCCCGTTCGGCAACCATCCTCGGGATCACGGTAATCGGCGGGCTGATCGCTTCGTATGTGCa                                                           <  2:1378530/238‑1 (MQ=255)
                                                                                                                                                                                            ttATGGC‑GGGAATTTGCTCA‑TCATTTGCCAGCCAG‑GGAAACCTGCT‑GGGGCCGATTCTA‑TTTTTCGCCGTTTACCTGCTTATCTTTTTCCTGCGCGTCGGC‑TGGACCCACGTCGGTTATTCAGTCGGCGTGAAGGCGATCGATAAAGTGCGAGAGAACTCGCAGATGATTGCCCGTTCGGCAACCATCCTCGGGATCACGGTAATCGGCGGGCTGATCGCTTCGTATGTGCATATTaa                                                     <  1:245157/238‑1 (MQ=255)
                                                                                                                                                                                            ttATGGC‑GGGAATTTGCTCA‑TCATTTGCCAGCCAG‑GGAAACCTGCT‑GGGGCCGATTCTA‑TTTTTCGCCGTTTACCTGCTTATCTTTTTCCTGCGCGTCGGC‑TGGACCCACGTCGGTTATTCAGTCGGCGTGAAGGCGATCGATAAAGTGCGAGAGAACTCGCAGATGATTGCCCGTTCGGCAACCATCCTCGGGATCACGGTAATCGGCGGGCTGATCGCTTCGTATGTGCATATTaa                                                     <  2:18826/238‑1 (MQ=255)
                                                                                                                                                                                                    gggAATTTGCTCA‑TCATTTGCCAGCCAG‑GGAAACCTGCT‑GGGGCCGATTCTA‑TTTTTCGCCGTTTACCTGCTTATCTTTTTCCTGCGCGTCGGC‑TGGACCCACGTCGGTTATTCAGTCGGCGTGAAGGCGATCGATAAAGTGCGAGAGAACTCGCAGATGATTGCCCGTTCGGCAACCATCCTCGGGATCACGGTAATCGGCGGGCTGATCGCTTCGTATGTGCATATTAACGTGGTGa                                             <  1:256500/239‑1 (MQ=255)
                                                                                                                                                                                                     ggAATTTGCTCA‑TCATTTGCCAGCCAG‑GGAAACCTGCT‑GGGGCCGATTCTA‑TTTTTCGCCGTTTACCTGCTTATCTTTTTCCTGCGCGTCGGC‑TGGACCCACGTCGGTTATTCAGTCGGCGTGAAGGCGATCGATAAAGTGCGAGAGAACTCGCAGATGATTGCCCGTTCGGCAACCATCCTCGGGATCACGGTAATCGGCGGGCTGATCGCTTCGTATGTGCATATTAACGTGGTGa                                             <  2:443584/238‑1 (MQ=255)
                                                                                                                                                                                                          ttGCTCA‑TCATTTGCCAGCCAG‑GGAAACCTGCT‑GGGGCCGATTCTA‑TTTTTCGCCGTTTACCTGCTTATCTTTTTCCTGCGCGTCGGC‑TGGACCCACGTCGGTTATTCAGTCGGCGTGAAGGCGATCGATAAAGTGCGAGAGAACTCGCAGATGATTGCCCGTTCGGCAACCATCCTCGGGATCACGGTAATCGGCGGGCTGATCGCTTCGTATGTGCATATTAACGTGGTGACATCGt                                       <  1:141383/239‑1 (MQ=255)
                                                                                                                                                                                                               ca‑tcatTTGCCATCCAG‑GGAAACCTGCT‑GGGGCAGATTCTA‑TTTTTCGCCGTTTACCTGCTTATCTTTTTCCTGCGCGTCGGC‑TGGACCCACGTCGGTTATTCAGTCGGCGTGAAGGCGATCGATAAAGTGCGAGAGAACTCGCAGATGATTGCCCGTTCGGCAACCATCCTCGGGATCACGGTAATCGGCGGGCTGATCGCTTCGTATGTGCATATTAACGTGGTGACATCGTTTGcc                                  <  2:1549682/239‑1 (MQ=255)
                                                                                                                                                                                                                  tcatTTGCCAGCCAG‑GGAAACCTGCT‑GGGGCCGATTCTA‑TTTTTCGCCGTTTACCTGCTTATCTTTTTCCTGCGCGTCGGC‑TGGACCCACGTCGGTTATTCAGTCGGCGTGAAGGCGATCGATAAAGTGCGAGAGAACTCGCAGATGATTGCCCGTTCGGCAACCATCCTCGGGATCACGGTAATCGGCGGGCTGATCGCTTCGTATGTGCATATTAACg                                                   <  2:103076/220‑1 (MQ=255)
                                                                                                                                                                                                                  tcatTTGCCAGCCAG‑GGAAACCTGCT‑GGGGCCGATTCTA‑TTTTTCGCCGTTTACCTGCTTATCTTTTTCCTGCGCGTCGGC‑TGGACCCACGTCGGTTATTCAGTCGGCGTGAAGGCGATCGATAAAGTGCGAGAGAACTCGCAGATGATTGCCCGTTCGGCAACCATCCTCGGGATCACGGTAATCGGCGGGCTGATCGCTTCGTATGTGCATATTAACg                                                   >  1:103076/1‑220 (MQ=255)
                                                                                                                                                                                                                  tcatTTGCCAGCCAG‑GGAAACCTGCT‑GGGGCCGATTCTA‑TTTTTCGCCGTTTACCTGCTTATCTTTTTCCTGCGCGTCGGC‑TGGACCCACGTCGGTTATTCAGTCGGCGTGAAGGCGATCGATAAAGTGCGAGAGAACTCGCAGATGATTGCCCGTTCGGCAACCATCCTCGGGATCACGGTAATCGGCGGGCTGATCGCTTCGTATGTGCATATTAACGTGGTGACATCGTTTGCCAt                                <  2:4898/239‑1 (MQ=255)
                                                                                                                                                                                                                  tcatTTGCCAGCCAG‑GGAAACCTGCT‑GGGGCCGATTCTA‑TTTTTCGCCGTTTACCTGCTTATCTTTTTCCTGCGCGTCGGC‑TGGACCCACGTCGGTTATTCAGTCGGCGTGAAGGCGATCGATAAAGTGCGAGAGAACTCGCAGATGATTGCCCGTTCGGCAACCATCCTCGGGATCACGGTAATCGGCGGGCTGATCGCTTCGTATGTGCATATTAACGTGGTGACATCGTTTGCCAt                                <  2:270905/239‑1 (MQ=255)
                                                                                                                                                                                                                  tcatTTGCCAGCCAG‑GGAAACCTGCT‑GGGGCCGATTCTA‑TTTTTCGCCGTTTACCTGCTTATCTTTTTCCTGCGCGTCGGC‑TGGACCCACGTCGGTTATTCAGTCGGCGTGAAGGCGATCGATAAAGTGCGAGAGAACTCGCAGATGATTGCCCGTTCGGCAACCATCCTCGGGATCACGGTAATCGGCGGGCTGATCGCTTCGTATGTGCATATTAACGTGGTGACATCGTTTGCCAt                                <  1:76455/239‑1 (MQ=255)
                                                                                                                                                                                                                    atTTGCCAGCCAG‑GGAAACCTTCT‑GGGGCCGATTCTA‑TTTTTCGCCGTTTACCTGCTTATCTTTTTCCTGCGCGTCGGC‑TGGACCCACGTCGGTTATTCAGTCGGCGTGAAGGCGATCGATAAAGTGCGAGAGAACTCGCAGATGATTGCCCGTTCGGCAACCATCCTCGGGATCACGGTAATCGGCGGGCTGATCGCTTCGTATGTGCATATTAACGTGGTGACATCGTTTGCCATCg                              <  1:1016507/239‑1 (MQ=255)
                                                                                                                                                                                                                        gccagccaG‑GGAAACCTGCT‑GGGGCCGATTCTA‑TTTTTCGCCGTTTACCTGCTTATCTTTTTCCTGCGCGTCGGC‑TGGACCCACGTCGGTTATTCAGTCGGCGTGAAGGCGATCGATAAAGTGCGAGAGAACTCGCAGATGATTGCCCGTTCGGCAACCATCCTCGGGATCACGGTAATCGGCGGGCTGATCGCTTCGTATGTGCATATTAACGTGGTGACATCGTTTGCCATCGACaa                          >  2:692489/1‑239 (MQ=255)
                                                                                                                                                                                                                             ccag‑GGTAACCTGCT‑GGGGCCGATTCTT‑TTTTTCCCCGTTTACCTGCTTATCCTTCTCCTGCTCGTCGGC‑TGGACCCACGTCGGTTATTCAGTCGTCGTGCAGGCGGTCGATAAACTACTAGAGCACTTGCAGTCTATTGCCCGTTCGGCAACCATTCCCGGGATCACGGTAATCGGCGGGCTTTTCGCTCCGTCTTCTCAAACTCTCGTGGTGCCATAGTTTGCCCTCgtacctatc                      >  2:1587874/1‑230 (MQ=255)
                                                                                                                                                                                                                             ccag‑GGAAACCTGCT‑GGGGCCGATTCTA‑TTTTTCGCCGTTTACCTGCTTATCTTTTTCCTGCGCGTCGGC‑TGGACCCACGTCGGTTATTCAGTCGGCGTGAAGGCGATCGATAAAGTGCGAGAGAACTCGCAGATGATTGCCCGTTCGGCAACCATCCTCGGGATCACGGTAATCGGCGGGCTGATCGCTTCGTATGTGCATATTAACGTGGTGACATCGTTTGCCAt                                >  2:365890/1‑228 (MQ=255)
                                                                                                                                                                                                                             ccag‑GGAAACCTGCT‑GGGGCCGATTCTA‑TTTTTCGCCGTTTACCTGCTTATCTTTTTCCTGCGCGTCGGC‑TGGACCCACGTCGGTTATTCAGTCGGCGTGAAGGCGATCGATAAAGTGCGAGAGAACTCGCAGATGATTGCCCGTTCGGCAACCATCCTCGGGATCACGGTAATCGGCGGGCTGATCGCTTCGTATGTGCATATTAACGTGGTGACATCGTTTGCCAt                                <  1:365890/228‑1 (MQ=255)
                                                                                                                                                                                                                             ccag‑GGAAACCTGCT‑GGGGCCGATTCTA‑TTTTTCGCCGTTTACCTGCTTATCTTTTTCCTGCGCGTCGGC‑TGGACCCACGTCGGTTATTCAGTCGGCGTGAAGGCGATCGATAAAGTGCGAGAGAACTCGCAGATGATTGCCCGTTCGGCAACCATCCTCGGGATCACGGTAATCGGCGGGCTGATCGCTTCGTATGTGCATATTAACGTGGTGACATCGTTTGCCATCGAc                            >  1:35170/1‑232 (MQ=255)
                                                                                                                                                                                                                             ccag‑GGAAACCTGCT‑GGGGCCGATTCTA‑TTTTTCGCCGTTTACCTGCTTATCTTTTTCCTGCGCGTCGGC‑TGGACCCACGTCGGTTATTCAGTCGGCGTGAAGGCGATCGATAAAGTGCGAGAGAACTCGCAGATGATTGCCCGTTCGGCAACCATCCTCGGGATCACGGTAATCGGCGGGCTGATCGCTTCGTATGTGCATATTAACGTGGTGACATCGTTTGCCATCGACAATAccc                     >  1:303962/1‑239 (MQ=255)
                                                                                                                                                                                                                             ccag‑GGAAACCTGCT‑GGGGCCGATTCTA‑TTTTTCGCCGTTTACCTGCTTATCTTTTTCCTGCGCGTCGGC‑TGGACCCACGTCGGTTATTCAGTCGGCGTGAAGGCGATCGATAAAGTGCGAGAGAACTCGCAGATGATTGCCCGTTCGGCAACCATCCTCGGGATCACGGTAATCGGCGGGCTGATCGCTTCGTATGTGCATATTAACGTGGTGACATCGTTTGCCATCGACAATAccc                     >  1:722444/1‑239 (MQ=255)
                                                                                                                                                                                                                             ccag‑GGAAACCTGCT‑GGGGCCGATTCTA‑TTTTTCGCCGTTTACCTGCTTATCTTTTTCCTGCGCGTCGGC‑TGGACCCACGTCGGTTATTCAGTCGGCGTGAAGGCGATCGATAAAGTGCGAGAGAACTCGCAGATGATTGCCCGTTCGGCAACCATCCTCGGGATCACGGTAATCGGCGGGCTGATCGCTTCGTATGTGCATATTAACGTGGTGACATCGTTTGCCATCGACAATAccc                     >  1:1201014/1‑239 (MQ=255)
                                                                                                                                                                                                                             ccag‑GGAAACCTGCT‑GGGGCCGATTCTA‑TTTTTCGCCGTTTACCTGCTTATCTTTTTCCTGCGCGTCGGC‑TGGACCCACGTCGGTTATTCAGTCGGCGTGAAGGCGATCGATAAAGTGCGAGAGAACTCGCAGATGATTGCCCGTTCGGCAACCATCCTCGGGATCACGGTAATCGGCGGGCTGATCGCTTCGTATGTGCATATTAACGTGGTGACATCGGTTGCCATTGACAATTccc                     >  1:1649168/1‑239 (MQ=255)
                                                                                                                                                                                                                             ccag‑GGAAACCTGCT‑GGGGCCGATTCTA‑TTTTTCGCCGTTTACCTGCTTATCTTTTTCCTGCGCGTCGGC‑TGGACCCACGTCGGTTATTCAGTCGGCGTGAAGGCGATCGATAAAGTGCGAGAGAACTCGCAGATGATTGCCCGTTCGGCAACCATCCTCGGGATCACGGTAATCGGCGGGCTGATCGCTTCGTATGTGCATATTAAAGTGGTGACATCGTTTGCCATCGACAATAcc                      >  1:301719/1‑238 (MQ=255)
                                                                                                                                                                                                                                           ct‑gGGGCCGATTCTA‑TTTTTCGCCGTTTACCTGCTTATCTTTTTCCTGCGCGTCGGC‑TGGACCCACGTCGGTTATTCAGTCGGCGTGAAGGCGATCGATAAAGTGCGAGAGAACTCGCAGATGATTGCCCGTTCGGCAACCATCCTCGGGATCACGGTAATCGGCGGGCTGATCGCTTCGTATGTGCATATTAACGTGGTGACATCGTTTGCCATCGACAATACCCACAGCGTTgc           >  2:271969/1‑236 (MQ=255)
                                                                                                                                                                                                                                            t‑gGGGCCGATTCTA‑TTTTTCGCCGTTTACCTGCTTATCTTTTTCCTGCGCGTCGGC‑TGGACCCACGTCGGTTATTCAGTCGGCGTGAAGGCGATCGATAAAGTGCGAGAGAACTCGCAGATGATTGCCCGTTCGGCAACCATCCTCGGGATCACGGTAATCGGCGGGCTGATCGCTTCGTATGTGCATATTAACGTGGTGACATCGTTTGCCATCGACAATACCCACAGCGTTGCGCTg       <  1:150123/239‑1 (MQ=255)
                                                                                                                                                                                                                                                 gCCGATTCTA‑TTTTTCGCCGTTTACCTGCTTATCTTTTTCCTGCGCGTCGGC‑TGGACCCACGTCGGTTATTCAGTCGGCGTGAAGGCGATCGATAAAGTGCGAGAGAACTCGCAGATGATTGCCCGTTCGGCAACCATCCTCGGGATCACGGTAATCGGCGGGCTGATCGCTTCGTATGTGCATATTAACGTGGTGACATCGTTTGCCATCGACAATACCCACAGTGTTGCGCTgtagc   >  2:918860/1‑239 (MQ=255)
                                                                                                                                                                                                                                                  ccGATTCTA‑TTTTTCGCCGTTTACCTGCTTATCTTTTTCCTGCGCGTCGGC‑TGGACCCACGTCGGTTATTCAGTCGGCGTGAAGGCGATCGATAAAGTGCGAGAGAACTCGCAGATGATTGCCCGTTCGGCAACCATCCTCGGGATCACGGTAATCGGCGGGCTGATCGCTTCGTATGTGCATATTAACGTGGTGACATCGTTTGCCATCGCCAATACCCACAGCGTTTCGCTgcagca  >  2:138516/1‑239 (MQ=255)
                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                
CGCGGCGATGAAAGATAACCTCGAATTTATTAATACCCACCCGAATCTGGTCGGATTCCTGATGGGGTTATTAATTTCGATGGAAGAAAAAGGAGAAAACCGCGACACCATTAAAGGCCTCAAAGTGGCACTGTTTGGCCCAATCGCCGGGATTGGCGATGCGATTTTCTGGTTTACTTTGTTGCCGATTATGGC‑GGGAATTTGCTCA‑TCATTTGCCAGCCAG‑GGAAACCTGCT‑GGGGCCGATTCTA‑TTTTTCGCCGTTTACCTGCTTATCTTTTTCCTGCGCGTCGGC‑TGGACCCACGTCGGTTATTCAGTCGGCGTGAAGGCGATCGATAAAGTGCGAGAGAACTCGCAGATGATTGCCCGTTCGGCAACCATCCTCGGGATCACGGTAATCGGCGGGCTGATCGCTTCGTATGTGCATATTAACGTGGTGACATCGTTTGCCATCGACAATACCCACAGCGTTGCGCTGCAGCA  >  NC_000913/3285639‑3286115

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑