Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	989,901	G→T	17.8%	S152R (AGC→AGA)	pncB ←	nicotinate phosphoribosyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	989,901	0	G	T	17.8%	148.7 / 4.9	84	S152R (AGC→AGA)	pncB	nicotinate phosphoribosyltransferase
Reads supporting (aligned to +/- strand):  ref base G (23/44);  new base T (13/3);  total (37/47)
Fisher's exact test for biased strand distribution p-value = 1.36e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.63e-01

GGGCTGATTTGCTGATGTGCCTGGAACCATTCGTGTGCCTGTGTTCCCATCGGCGTGAGGGAAAGCCGACGCGCCAGATCGTAGTTGCTGGTGCCCACAAACCAGGATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCGAC‑GGGTGCCAAAATCCATCAGATGGAAGCGCGACATATC‑AAGACCGGC‑GGTT‑AACGCCGAGAAGTCGACTAA‑TTTGC‑TTTCCA‑GCGTGTCGA‑GGGCTTGCGCAACGTCGGCCTGCGGTGAGCGATAGCGATGTACCA‑TTTCACTGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCTTAAAGAAAGGCAGGGCAGAAAGC  >  NC_000913/989665‑990138                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                  gggCTGATTTGCTGATGTGCCTGGAACCATTCGTGTGCCTGTGTTCCCATCGGCGTGAGGGAAAGCCGACGCGCCAGATCGTAGTTGCTGGTGCCCACAAACCAGGATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCGAC‑GGGTGCCAAAATCCATCAGATGGAAGCGCGACATATC‑AAGACCGGC‑GGTT‑AACGCCGAGAAGTCGACTAA‑TTTGC‑t                                                                                                                                                                                                                                                <  2:397332/239‑1 (MQ=255) gggCTGATTTGCTGATGTGCCTGGAACCATTCGTGTGCCTGTGTTCCCATCGGCGTGAGGGAAAGCCGACGCGCCAGATCGTAGTTGCTGGTGCCCACAAACCAGGATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCGAC‑GGGTGCCAAAATCCATCAGATGGAAGCGCGACATATC‑AAGACCGGC‑GGTT‑AACGCCGAGAAGTCGACTAA‑TTTGC‑t                                                                                                                                                                                                                                                <  1:166196/239‑1 (MQ=255)       aTTTGCTGATGTGCCTGGAACCATTCGTGTGCCTGTGTTCCCATCGGCGTGAGGGAAAGCCGACGCGCCAGATCGTAGTTGCTGGTGCCCACAAACCAGGATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCGAC‑GGGTGCCAAAATCCATCAGATGGAAGCGCGACATATC‑AAGACCGGCGGGTT‑AAGGCGGAAAGGTGGAC‑AA‑TTTGC‑TTTTCA‑g                                                                                                                                                                                                                                         >  1:139380/1‑239 (MQ=255)       aTTTGCTGATGTGCCTGGAACCATTCGTGTGCCTGTGTTCCCATCGGCGTGAGGGAAAGCCGACGCGCCAGATCGTAGTTGCTGGTGCCCACAAACCAGGATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCGAC‑GGGTGCCAAAATCCATCAGATGGAAGCGCGACATATC‑AAGACCGGC‑GGTT‑AAAGACAAGAACTGGACTCA‑TTTAC‑TTTccc                                                                                                                                                                                                                                           >  2:91303/1‑237 (MQ=255)       aTTTGCTGATGTGCCTGGAACCATTCGTGTGCCTGTGTTCCCATCGGCGTGAGGGAAAGCCGACGCGCCAGATCGTAGTTGCTGGTGCCCACAAACCAGGATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCGAC‑GGGTGCCAAAATCCATCAGATGGAAGCGCGACATATC‑AAGACCGGC‑GGGT‑AACGCCGAGGAGTCGCCTAA‑TTTGC‑TTTCCa                                                                                                                                                                                                                                           >  1:618671/1‑238 (MQ=255)       aTTTGCTGATGTGCCTGGAACCATTCGTGTGCCTGTGTTCCCATCGGCGTGAGGGAAAGCCGACGCGCCAGATCGTAGGTGCTGGTGCCCACAAACCAGGATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGCAAAACGGCGAC‑GGGTGCCAAAATCCATCAGATGGAAGCGCGACATATC‑AAGACCGGC‑GGGT‑AAAGGCCAGAAGACGACCAA‑TATGC‑TCTCCa                                                                                                                                                                                                                                           >  2:975352/1‑238 (MQ=255)        tttGGTGATGTGCCTGGAACCAGTCGTGTGCCTGTGTTCCCATCGGCGTGAGGGAAAGCCGACGCGCCAGATCGTAGTTGCTGGTGCCCACAAACCAGGATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCGAC‑GGGTGCCAAAATCCATCAGATGGAAGCGCGACATATC‑AAGACCGGC‑GGTT‑AACGCCGAGAAGTCGACTAA‑TTTGC‑TTTCCA‑Gc                                                                                                                                                                                                                                        <  2:331387/239‑1 (MQ=255)              gATGTGCCTGGAACCATTCGTGTGCCTGTGTTCCCATCGGCGTGAGGGAAAGCCGACGCGCCAGATCGTAGTTGCTGGTGCCCACAAACCAGGATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCGAC‑GGGTGCCAAAATCCATCAGATGGAAGCGCGACATATC‑AAGACCGGC‑GGTT‑AACGCCGAGAAGTCGACTAA‑TTTGC‑TTTCCA‑GCGTGTCg                                                                                                                                                                                                                                  <  1:820805/239‑1 (MQ=255)                 gtgCCTGGAACCATTCGTGTGCCTGTGTTCCCATCGGCGTGAGGGAAAGCCGACGCGCCAGATCGTAGTTGCTGGTGCCCACAAACCAGGATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGCAAAACGGCGAC‑GGGTGCCAAAATCCATCAGATGGAGGCCCAAAAAAT‑‑AAGACCGGCGGGTT‑AAACCCGCGAAGTCGACTAC‑TTTGC‑TTTCAC‑CCGTGTCgg‑gg                                                                                                                                                                                                                              >  1:373052/1‑239 (MQ=255)                 gtgCCTGGAACCATTCGTGTGCCTGTGTTCCCATCGGCGTGAGGGAAAGCCGACGCGCCAGATCGTAGTTGCTGGTGCCCACAAACCAGGATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCGAC‑GGGTGCCAAAATCCATCAGATGGAAGCGCGACATATC‑AAGACCGGC‑GGGT‑AAAGCCGAAGAGGCGAACAA‑TTTTC‑TTTTCA‑GCggggcgggg                                                                                                                                                                                                                               >  1:1223854/1‑231 (MQ=255)                 gtgCCTGGAACCATTCGTGTGCCTGTGTTCCCATCGGCGTGAGGGAAAGCCGACGCGCCAGATCGTAGTTGCTGGTGCCCACAAACCAGGATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCGAC‑GGGTGCCAAAATCCATCAGATGGAAGCGCGAACTATC‑AAGACCGGC‑GGTTAAACACCG‑GAAGGCGACTTA‑TTTTG‑CTTCCA‑GGGTGGTGA‑gg                                                                                                                                                                                                                              >  1:652504/1‑239 (MQ=255)                    gcTGGACCCGTTCGTGTGCCTGTGTTCCCATCGGCGTGAGGGAAAGCCGACGCGCCAGATCGTAGTTGCTGGTGCCCACAAACCAGGATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTGCGCGAGAAAAACGGCGAC‑GGGTGCCAAAATCCATCAGATGGAAGCGCGACATATC‑AAGACCGGC‑GGTT‑AACGCCGAGAAGTCGACTAA‑TTTGC‑TTTCCA‑GCGTGTCGA‑GGGc                                                                                                                                                                                                                            <  2:673824/237‑1 (MQ=255)                             gttCGGGTGCCGGTGTTCCCATCGGCGTGAGGGAAAGCCGACGCGACAGATCGTAGTTGCTGGTGCCCACAAACCAGGATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCGAC‑GGGTGCCAAAATCCATCAGATGGAAGCGCGACATATC‑AAGACCGGC‑GGTT‑AACGCCGAGAAGTCGACTAA‑TTTGC‑TTTCCA‑GCGTGTCGA‑GGGCTTGCGCAACg                                                                                                                                                                                                                  <  2:604956/238‑1 (MQ=255)                             aTTCGTGTGCCTGGGTTCCCATCGGCGTGAGGGAAAGCCGACGCGCCAGATCGTAGTTGCTGGTGCCCACAAACCAGGATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCGAC‑GGGTGCCAAAATCCATCAGATGGAAGCGCGACATATC‑AAGACCGGC‑GGTT‑AACGCCGAGAAGTCGACTAA‑TTTGC‑TTTCCA‑GCGTGTCGA‑GGGCTTGCGCAACg                                                                                                                                                                                                                  <  2:649394/239‑1 (MQ=255)                              ttCGTGTGCCTGTGTTCCCATCGGCGTGAGGGAAAGCCGACGCGCCAGATCGTAGTTGCTGGTGCCCACAAACCAGGATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAACAACGGCGAC‑GGGCGCCAAAATCCATCAGATGGAAGCGCGACATATC‑AAGACCGGC‑GGTT‑AAAGCCGAAAAAGCGACCAA‑ATTTA‑TTTACA‑ACCTGTCGA‑GGGCtgtgcccacg                                                                                                                                                                                                                  >  2:644759/1‑229 (MQ=255)                              ttCGTGTGCCTGTGTTCCCATCGGCGTGAGGGAAAGCCGACGCGCCAGATCGTAGTTGCTGGTGCCCACAAACCAGGATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCGAC‑GGGTGCCAAAATCCATCAGAGGGAAGCGCGACATATC‑AAGACCGGC‑GGCT‑AAAGCCGAGACGGCGACTAG‑TTTGA‑TTTTCA‑CAGGGGCGA‑GGGGTTgcgccaag                                                                                                                                                                                                                  >  2:1080338/1‑234 (MQ=255)                                        tgtgTTCCCTGCGGCGTGGGGGAAATCCGACCGGCCAGATCGTAGTTGCTGGTGCCCACAAACCAGGATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTAGCGAGAAAAACGGCGAC‑GGGTGCCAAAATCCATCAGATGGAAGCGCGACATATC‑AAGACCGGC‑GGTT‑AACGCCGAGAAGTCGACTAA‑TTTGC‑TTTCCA‑GCGTGTCGA‑GGGCTTGCGCAACGTCGGCCTGCgg                                                                                                                                                                                                       <  2:634334/239‑1 (MQ=255)                                           gtTCCCATCGGCGTGAGGGAAAGCCGACGCGCCAGATCGTAGTTGCTGGTGCCCACAAACCAGGATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCGAC‑GGGTGCCAAAATCCATCAGATGGAAGCGCGACATATC‑AAGACCGGC‑GGTT‑AACGCCGAGAAGTCGACTAA‑TTTGC‑TTTCCA‑GCGTGTCGA‑GGGCTTGCGCAACGTCGGCCTGCGGTg                                                                                                                                                                                                     <  2:348975/238‑1 (MQ=255)                                           gtTCCCATCGGCGTGAGGGAAAGCCGACGCGCCAGATCGTAGTTGCTGGTGCCCACAAACCAGGATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCGAC‑GGGTGCCAAAATCCATCAGATGGAAGCGCGACATATC‑AAGACCGGC‑GGTT‑AACGCCGAGAAGTCGACTAA‑TTTGC‑TTTCCA‑GCGTGTCGA‑GGGCTTGCGCAACGTCGGCCTGCGGTg                                                                                                                                                                                                     <  2:666187/238‑1 (MQ=255)                                             tCCCATCGGCGTGAGGGAAAGCCGACGCGCCAGATCGTAGTTGCTGGTGCCCACAAACCAGGATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCGAC‑GGGTGCCAAAATCCATCAGATGGAAGCGCGACATATC‑AAGACCGGC‑GGTT‑AACGCCGAGAAGTCGACTAA‑TTTGC‑TTTCCA‑GCGTGTCGA‑GGGCTTGCGCAACGTCGGCCTGCGGTGag                                                                                                                                                                                                   <  2:116440/238‑1 (MQ=255)                                                  tCGGCGTGAGGGAAAGCCGACGCGCCAGATCGTAGTTGCTGGTGCCCACAAACCAGGATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCGAC‑GGGTGCCAAAATCCATCAGATGGAAGCGCGACATATC‑AAGACCGGC‑GGTT‑AACGCCGAGAAGTCGACTAA‑TTTGC‑TTTCCA‑GCGTGTCGA‑GGGCTTGCGCAACGTCGGCCTGCGGTGagcgatag                                                                                                                                                                                             <  1:900339/239‑1 (MQ=255)                                                    ggCGTGAGGGAAAGCCGACGCGCCAGATCGTAGTTGCTGGTGCCCACAAACCAGGATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCGAC‑GGGTGCCAAAATCCATCAGATGGAAGCGCGACATATC‑AAGACCGGC‑GGTT‑AACGCCGAGAAGTCGACTAA‑TTTGC‑TTTCCA‑GCGTGTCGA‑GGGCTTGCGCAACGTCGGCCTGCGGTGagcgatagcg                                                                                                                                                                                           <  1:251466/239‑1 (MQ=255)                                                      cGTGAGGGAAAGCCGACGCGCCAGATCGTAGTTGCTGGTGCCCACAAACCAGGATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCGAC‑GGGTGCCAAAATCCATCAGATGGAAGCGCGACATATC‑AAGACCGGC‑GGGT‑AAAGCCGAGAAAGCGGATAA‑ATTGC‑TTTCCA‑GCGGGTCGA‑GGGGTTGCGCAAAGTCGGCCTGCGGGGggcgagagcggt                                                                                                                                                                                         >  1:890454/1‑237 (MQ=255)                                                       gTGAGGGAAAGCCGACGCGCCAGATCGTAGTTGCTGGTGCCCACAAACCAGGATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCGAC‑GGGTGCCAAAATCCATCAGATGGAAGCGCGACATATC‑AAGACCGGC‑GGTT‑AACGCCGAGAAGTCGACTAA‑TTTGC‑TTTCCA‑GCGTGTCGA‑GGGCTTGCGCAACGTCGGCCTGCGGTGAGCGATAGCGATg                                                                                                                                                                                        <  2:444842/239‑1 (MQ=255)                                                       gTGAGGGAAAGCCGACGCGCCAGATCGTAGTGGCTGGTGCCCACAAACCGGGATTCCTGTTGCAGACGCTTAACGATGGCTTCTTGTACCTCGCGAGAAAAACGGCGAC‑GGGTGCCAAAATCCATCAGATGGAAGCGCGACATATC‑AAGACCGGC‑GGTT‑AACGCCGAGAAGTCGACTAA‑TTTGC‑TTTCCA‑GCGTGTCGA‑GGGCTTGCGAAACGTCGGCCTGCGGTGAGCGATAGCGATg                                                                                                                                                                                        <  2:420003/239‑1 (MQ=255)                                                        tGAGGGAAAGCCGACGCGCCAGATCGTAGTTGCTGGTGCCCACAAACCAGGATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCGAC‑GGGTGCCAAAATCCATCAGATGGAAGCGCGACATATC‑AAGACCGGC‑GGTT‑AACGCCGAGAAGTCGACTAA‑TTTGC‑TTTCCA‑GCGTGTCGA‑GGGCTTGCGCAACGTCGGCCTGCGGTGAGCGATAGCGATg                                                                                                                                                                                        <  2:789900/238‑1 (MQ=255)                                                                aGCCGACGCGCCAGATCGTAGTTGCTGGTGCCCACAAACCAGGATT‑CTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCGAC‑GGGTGCCAAAATCCATCAGATGGAAGCGCGACATATC‑AAGACCGGC‑GGTT‑AAAGCCCGGAAGGAGAATAATTTTGT‑TTTCCA‑‑CGTGGCGG‑GGGGTTGTGCAAAGTCGGCCTGGGGGGAGCGTAAGCGGTGGACCC‑tttt                                                                                                                                                                              >  1:1118632/1‑237 (MQ=255)                                                                     aCGCGCCAGATCGTAGTTGCTGGTGCCCACAAACCAGGATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCGAC‑GGGTGCCAAAATCCATCAGATGGAAGCGCGACATATC‑AAGACCGGC‑GGTT‑AACGCCGCGAAGTCGAACAA‑TTTGC‑TTTCCA‑GCGGGTGGA‑GGGCGTGCGCAACGTCCGCCTGCGGGGGGGGCTAGCCGGGTGCCA‑TTTCCCttg                                                                                                                                                                         >  2:1025507/1‑237 (MQ=255)                                                                      cgcgCCAGATCGTAGTTGCTGGTGCCCACAAACCAGGATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCGAC‑GGGTGCCAAAATCCATCAGAGGGAAGCGCGACATATC‑AAGACCGGC‑GGTT‑AACGCCGAGAAGTCGACTAA‑TTTGC‑TTTCCA‑GCGTGTCGA‑GGGCTTGCGCAACGTCGGCCTGCGGTGAGCGATAGCGATGTACCA‑TTTCACTGa                                                                                                                                                                         <  1:91303/238‑1 (MQ=255)                                                                        cgcCAGATCGTAGTTGCTGGTGCCCACAAACCAGGATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCGAC‑GGGTGCCAAAATCCATCAGATGGAAGCGCGACATATC‑AAGACCGGC‑GGTT‑AACGCCGGGAAGTCGGCTCA‑TTTTC‑TTTTCA‑GCGTGTCGG‑GGGGTTGCGGAAACGCGGGCTGCCGGGGGCGGCAGCGGTGTACCC‑TTTCTCTGATa                                                                                                                                                                       >  2:1126734/1‑238 (MQ=255)                                                                             acgcGTAGTTGCGGGGCCCCACAAACCAGGATACCGTTGGCAGCCGTTTAACGATGGTTTTTTGTACTTCGCGAGAAAAACGGCGCC‑GGGGGCCAAAACCCATCAGATGGAAGCGCGACATACC‑AAGCCCGGC‑GGTT‑ACCGCCGAGAAGTCGACTAA‑TTTGC‑TTTCCA‑GCGTGGCGA‑GGGCTTGCGCAACTCCGGCCTGCGGTGAGCGATAGCGATGTACCA‑TTTCACTGATAACCGcc                                                                                                                                                                 <  2:1036234/236‑1 (MQ=255)                                                                                   aGTTGCTGGTGCCCACAAACCAGGATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAACCGGCGAC‑GGGTGCCAAAACCCATCAGATGGAAGCGCGACATATC‑AAGACCGGC‑GGTT‑AACGCCGAGAAGTCGACTAA‑TTTGC‑TTTCCA‑GCGTGTCGA‑GGGCTTGCGCAACGTCGGCCTGCGGTGAGCGATAGCGATGTACCA‑TTTCACTGATAACCGCCAGCaa                                                                                                                                                            <  1:952787/238‑1 (MQ=255)                                                                                   aGTTGCTGGTGCCCACAAACCAGGATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCGAC‑GGGTGCCAAAATCCATCAGATGGAAGCGCGACATATC‑AAGACCGGC‑GGTT‑AACGCCGAGAAGGCGGCTAA‑TTTGC‑TTTCCA‑GCGTGTCGA‑GGGGTTGCGCCACGTCGGCCCGCGGGGAGCGATAGCGATGTACCA‑TTTCACTGATAAACGCCCGCaaa                                                                                                                                                           >  1:1005922/1‑239 (MQ=255)                                                                                          ggTGCCCACAAACCAGGATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCGAC‑GGGTGCCAAAATCCATCAGATGGAAGCGCGACATATC‑AAGACCGGC‑GGTT‑AACGCCGAGAAGTCGACTAA‑TTTGC‑TTTCCA‑GCGTGTCGA‑GGGCTTGCGCAACGTCGGCCTGCGGGGAGCGGTAGCGATGTACCC‑TTTCACTGATAACCGCCCGCAAAGGAACt                                                                                                                                                     >  1:477396/1‑238 (MQ=255)                                                                                          ggTGCCCACAAACCAGGATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCGAC‑GGGTGCCAAAATCCATCAGATGGAAGCGCGACATATC‑AAGACCGGC‑GGTT‑AAAGCCGAGAAGTCGACTAA‑TTTGC‑TTTTCA‑GCGTGTCGA‑GGGCTTGCGCAACGTCGGCCTGCGGTGAGCGGTAGCGATGTAACA‑TTTCACTGATAAACCCCCGCCAAGGaaat                                                                                                                                                     >  2:1083384/1‑236 (MQ=255)                                                                                                               cTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCGAC‑GGGTGCCAAAATCCATCAGATGGAAGCGCGACATATC‑AAGACCGGC‑GGTT‑AACGCCGAGAAGTCGACTAA‑TTTGC‑TTTCCC‑GCGTGTCGG‑GGGCGTGCGCAACGGCGGCCTGCGGTGGGCGATAGCGATGTACCA‑TTTCACTGATAACCGCCCGCAAAGGAACATCCCAGAGGGTGAATTCACGc                                                                                                                                >  1:1147389/1‑238 (MQ=255)                                                                                                                 gTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCGAC‑GGGTGCCAAAATCCATCAGATGGAAGCGCGACATATC‑AAGACCGGC‑GGGT‑AAAGCCGCGAAGTCGACTAA‑ATTGC‑TTTTCA‑GCGGGGCGG‑GGGCCTGCGCAAAGTCGGCCTTCGGTGTGCGGTAGCGGAGGACCA‑GTTTAATGATAAACCCCCGGCAAGGAACTTTCCCGGGGATGGATTTACGccc                                                                                                                              >  2:563338/1‑237 (MQ=255)                                                                                                                   tgaaGACGGTTACGGAGGGTTTTTTGTACTGCGCGAAAAAACGGGCGAG‑GGGTGCAAAAATCCATCAGAGGGAAGCGCGACATACC‑AAGACCGGC‑GGTT‑AACGCCGAGAAGTCGACTAA‑TTTGC‑TTCCCA‑GCGTGTGGA‑GGGCTTGCGCAACGTCGGCCTGGGGTGAGCGATAGCGATGTACCA‑TTTCACTGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACg                                                                                                                            <  2:696236/235‑1 (MQ=255)                                                                                                                    gCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCGAC‑GGGTGCCAAAATCCATCAGATGGAAGCGCGACATATC‑AAGACCGGC‑GGTT‑AACGCCGAGAAGTCGACTAA‑TTTGC‑TTTTCA‑GCGTGTCGG‑GGGGTTGCGCAACGACCGCCTGCCGGGGGCGGTAGCGCTGGAACA‑TTTCACTCATAACCGCCCGCAAAGGCACTTCCCCGCGGGTGACCTctcccccccg                                                                                                                           >  2:373855/1‑229 (MQ=255)                                                                                                                       gACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCGAC‑GGGTGCCAAAATCCATCAGATGGAAGCGCGACATATC‑AAGACCGGC‑GGTT‑AACGCCCGGAAGTCGACTAA‑TTTGC‑TTTCCA‑GCGGGTCGG‑GGGGTTGCGCCAAGGCGGCCCGCGGGGAGCGCAAGCGATGTAACC‑TTTCTCTGATAAACCCCAGCAAAGGGAATTCCCCGGGGATGACTTCACGCCCCGGGcc                                                                                                                        >  2:383357/1‑238 (MQ=255)                                                                                                                       gACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCGAC‑GGGTGCCAAAATCCATCAGATGGAAGCGCGACATATC‑AAGACCGGC‑GGTT‑AACGCCCAGAAGTCGACTAA‑TTTTC‑TTTCCA‑GCGCGTCGG‑GGGCTTGCGCCACGTCGGCCCGCGGGGAGCGATAGCGATGTACCA‑TTTCACTGATAACCGCCCGCAAAGGAACTTCCCCGAGGATGACTTCACGCCACGGGcc                                                                                                                        >  1:590856/1‑238 (MQ=255)                                                                                                                                        tCTTGTACTTCGCGAGAAAAACGGCGAC‑GGGTGCCAAAATCCATCAGATGGAAGCGCGACATATC‑AAGACCGGC‑GGTT‑AAAGCCGCGAAATCGCCTAA‑TTTTC‑TTTCCA‑GCGTGTCGG‑GGGCTTGCGCCACCGCGGCCTGCGGGGAGCGGTAGCGGTGTAACA‑TTTCACTGGTAACCCCCCCCCAAAGAACTTTCCCGAGGGTGAATTTTAGCCCCGGGCCCCTTTAAAGAatttaca                                                                                                       >  2:378376/1‑233 (MQ=255)                                                                                                                                          tttgaCTGCGCGAAAAAAGCGGCTAC‑GGGTCCCAATATCCAACAGAGGGAAGCGCGACAAATA‑GAGACCGGC‑GGTT‑AACGCCGAGAGGTCGACTAA‑TTTGC‑TTCCCA‑GCGGGTCGA‑GGGTTTGCGCAATTTCGGCCTGCGGTGAGCGATGAAGTTGTACAA‑TTTCCCTGAAACCCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCt                                                                                                    <  2:800021/235‑1 (MQ=255)                                                                                                                                                ttCGCGAGAAAAACGGCGAG‑GGGTGCCAAAATCCATCAGAGGGAAGTG‑GAAATACCAAAGACCCGG‑GGTT‑AGCGCAGGAAGGTCCA‑TTA‑TTTTT‑TTTCCACGGTTCTCGA‑GGGCTTGCGAAACTTCGGCTTGGGGGGGGGGATGGGAATGTACCA‑TTTCACTGAAAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCAt                                                                                              <  2:418862/239‑1 (MQ=255)                                                                                                                                                tgcgcgAGAAAACCGGGGGC‑GGGGGAAAAAACCAAACGAATGGAAGCGGGACATATC‑AGGCCGGGC‑GGTT‑ACCGCCGAGAAGCCGACTAA‑TTTGC‑TTTCCA‑GCGTGTGGA‑GGGTTTGCGCAACGTCGGCCGGGGGTGAGCGAGAGCGATGTACCA‑TTTCACTGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCAt                                                                                              <  2:259729/237‑1 (MQ=255)                                                                                                                                                  ggcgAG‑AAAACGGCGGCGGGGGGCCAAAAGCCATCAGACGAAAGCGCGACATTTC‑AAGCCCGGC‑GGTA‑AACGCCGAGAAGCCGCTTAT‑TTTGC‑TTCCAA‑GCGTGTGGA‑GGCCTTGCGCAACGTCGGCGTGCGGTGAGCGATAGGGATGTACCT‑TTTCCCTGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTa                                                                                            <  2:606028/238‑1 (MQ=255)                                                                                                                                                  cgcgAGAAAAACGGCGAC‑GGGTGCCAAAATCCATCAGATGGAAGCGCGACATATC‑AAGACCGGC‑GGTT‑AACGCCGCGAAGTCGACTAA‑TTTTC‑TTTCCA‑GCGTGTCGA‑GGGCTTGCGCAACGTCGGCCTGCGGTGAGCGGTAGCGATGTACCA‑TTTCACTGATAACCCCCCGCAAAGGAACTTCCCAGAGGGTGACTTCACGCCACGGGGCGCCTAAAAGGAAATCCAACTTGCCAttt                                                                                            >  1:1103764/1‑238 (MQ=255)                                                                                                                                                  cgcgAGAAAAACGGCGAC‑GGGTGCCAAAATCCATCAGATGGAAGCGCGACATATC‑AAGACCGGC‑GGTT‑AACGCCCAGAAGTCGACTAA‑TTTTC‑TTTTCA‑GCCGGTCGA‑GGGCTTGCGCAACCACGGCCTGCGGGGAGCGATAGCGGTGTACCA‑TTTTACTTATAAACCCCCGCAAAAGAAATTCCCACAGGAAGACTTCACCCCACCGGCCGACTAAAAGAATATCCAACCTGTCATTa                                                                                            >  2:737493/1‑239 (MQ=255)                                                                                                                                                  cgcgAGAAAAACGGCGAC‑GGGTGCCAAAATCCATCAGATGGAAGCGCGACATATC‑AAGACCGGC‑GGTT‑AAAGCCGAGAAGTCGACTAA‑TTTGC‑TTTTCA‑GCGTGTCCA‑GGGCTTGGGAAACATCGGCCTGCGGTGAGAGCTAAGGCTGTTCCA‑TTTCACTTAATAACGCCACACAAGAAACATCCCCGCGGGTGACCTCAACCCCCGCGGCCCTTAAACGGAAATCCaacctcacctta                                                                                            >  2:742550/1‑228 (MQ=255)                                                                                                                                                  cgcgAGAAAAACGGCGAC‑GGGTGCCAAAATCCATCAGATGGAAGCGCGACATATC‑AAGACCGGC‑GGGT‑AACACCGAAAAGTAGACCAA‑TTTGC‑TTTTCA‑ACGTGGTGA‑AGGCTTTCGCAAAGTCGGGCTGCGGTTCGGGGTAGCGCGGAACCA‑GCTCATCGATTACCGCCACCAAACGAAGTTCCCCGAGGATGACGTCACTCCACGGCACCCTTAAAAGAATATCCAGCTTGGCAtgt                                                                                            >  2:699412/1‑237 (MQ=255)                                                                                                                                                  cgcgAGAAAAACGGCGAC‑GGGTGCCAAAATCCATCAGATGGAAGCGCGACATATC‑AAGACCGGC‑GGCT‑AACGCCGAGAAGTCGACTAA‑TTTGC‑TTTCCA‑GCCTGTCGA‑GGGGTTTCGCAAAGTCGGCCTGCGGGGGGCGCTAGCGATGTAACA‑TTTCACTGATAACCCCCAGCAAAGGAACTTCCCAGAGGATGACTTTACCCCCCGGGCCGCCTAAAAGAATATCCAGCTTGGCATTa                                                                                            >  2:125872/1‑239 (MQ=255)                                                                                                                                                    cgAGAAAAACGGCGAC‑GGGTGCCAAAATCCATCAGATGGAAGCGCGACATATC‑AAGACCGGC‑GGTT‑AACGCCGAGAAGTCGACTAA‑TTTGC‑TTTCCA‑GCGTGTCGA‑GGGCTTGCGCAACGTCGGCCCGCGGTGAGCGATAGCGATGTACCA‑TTTCACTGTTAACCGCCCGCAAAGGAACTTCCCAGAGGGTGACTTCACCCCACGGGCCGCCTAAAAGAAAAATCAGCTTTCCATTATc                                                                                          >  1:105888/1‑239 (MQ=255)                                                                                                                                                        aaaaaCGGCGGG‑GGGTCGCAAACCCCACAAGATGGAACGGCGACATACA‑AAAGCGGGC‑GGTT‑AACGCAGAGAAGTCG‑CTTA‑TTTGCTTTTCCA‑GCGTGTGGA‑GGATTTGGCCAAGGTCGGCCTGGGGTGGGCGAAAGGGATGTCCCA‑TTTCATGGAGAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTg                                                                                      <  2:136731/239‑1 (MQ=255)                                                                                                                                                                                      agagGGAAGGGGGACATAAC‑AAGACGGGG‑GGTA‑ACCGCGGAGAAGCCGACTAT‑TTTGC‑TTCCAG‑GGGGGGCGG‑GGGCTGGCGAAACGTCGGCCGGCGGGGGGCGATGGCGATGTCCCA‑TTCCACTGATACCCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAg                                                           <  2:590856/237‑1 (MQ=255)                                                                                                                                                                                          ggAAGCGCGACATATC‑AAGACCGGC‑GGTT‑AACGCCGAGAAGGCGACTAA‑TTTGC‑TTTCCA‑GCGTGTCGA‑GGGCGTGCGCAACGTCGGCCTGCGGTGAGCGATAGCGATGTACCA‑TTTCACTGATAAACACCCGCAAAAGAACTTCCCAAAGGGAGACTTCACGCCCCCGGGCGCCTAAAAGAAAATCCACCTCGCCATTATCGTTTGTCACGGTGACTTGTTTCGGGTTTAAGCGGaa                                                      >  2:79422/1‑238 (MQ=255)                                                                                                                                                                                            aaGCGCGACATATC‑AAGACCGGC‑GGTT‑AAAGCCGAGAAGTCGACTAA‑TTTTC‑TTTCCA‑GCGTGTCGA‑GGGCTTGCGCAAAGTCGGCCCGCGGGGGGCGAAAGCGATGTACCA‑TTTCACTGATAACCGCCAACAAAGGAACTTCCCAGAGGGTGACTTCACGCCCCCGGCCGCTTCAACGAAAATCCCACTTTCCATTTTCGTTTGACACGGTGACTTGGTCCGGGGTAAAGCGGaaacc                                                   >  2:48225/1‑236 (MQ=255)                                                                                                                                                                                                 cgACATATC‑AAGACCGGC‑GGTT‑AAAGCCGAGAAGTCGACTAA‑TTTTC‑TTTTCC‑GCGTGTCGA‑GGGCTTGCGCAACGTCGGCCTGCGGGGAGCGATAGCGATGTACCA‑TTTTACTGATAACCGCCCGCAAAAGAAATTCCCAGAGGGTGACTTCCCGCCACGGGCCCCTTAAAAGAATATCCAGCTTTCCATTATCGTTGGACACGGTGACTTGTTCCGGGGTTAAGCGGAACTCGCGTa                                              >  1:822188/1‑239 (MQ=255)                                                                                                                                                                                                  gacaagtC‑AAGACCGGG‑GGAT‑AACGCCGAGAAGTCGACGAT‑TTTGC‑TTTCCG‑GGGGGTCGA‑GGGCTTGCGCAACGCCGGCCTGCGGTGGGTGATAGCGATGTACCA‑TTTCACTGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTaa                                             <  2:671591/233‑1 (MQ=255)                                                                                                                                                                                                  gACATTAC‑AAGCCGGGG‑GGTT‑ATCGCCGAGAGGTCGGCTAA‑TTGTC‑TTGCCG‑TCTGTTCGG‑GGGCTGGCAAAACGCCGCCGGGGGGGGGGCGATAGCGTGGTACCA‑TTCCTCTGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTaa                                             <  2:1118632/239‑1 (MQ=255)                                                                                                                                                                                                  gACATATC‑AGGCCCGGC‑GGTT‑AACGCCGAGAAGTCGACTAA‑TTTGC‑TTTCCA‑GCGTGTCGA‑GGGCTGGCGCAACGTCGGCCTGCGGTGGGCGATAGCGATGTCCCT‑TTTCACGGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTaa                                             <  1:48225/239‑1 (MQ=255)                                                                                                                                                                                                   atatatA‑AAGACCGGC‑GCTC‑ACGGGCGAGAAGTCGCCTAA‑TTTGC‑TCTCCA‑GCGTTTCGA‑GGGCTCGCGCAAGGTGGGCCGGCGGTGAGCCAAGGCGATGTACTA‑TTTCACTGAGCGCCGCCAGCAAAGGAACTTCCCAGAGGATGACTGCGCGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTaa                                             <  2:608915/236‑1 (MQ=255)                                                                                                                                                                                                    acagtaaaggcccGGG‑GTTT‑CACGCCGAGAAGCCACTTAT‑TTTTC‑TTCCCT‑GCGTGGCGT‑GGGTTTGCGAAACGGCGGCCTGGGGTGAGTGAATGCGTGTTACCA‑TTTAACTGAGACCCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTaa                                             <  2:128173/227‑1 (MQ=255)                                                                                                                                                                                                    aaatacgaaGACGGGC‑GGTT‑AGCGCCGAGAAGTCGGCTAT‑TTTGC‑TTCCCG‑GCGTGGCGA‑GGTCTTGCGAAACGCCGGCCTGGGGTGAGCGATAGCGAGTTACCA‑TTTCACTAATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTaa                                             <  2:1147389/231‑1 (MQ=255)                                                                                                                                                                                                     aatagcagacccGGA‑GTTT‑AACGCCGAAAAGTCGTCTAT‑TTTGC‑TTTCCG‑GCGTGTCGA‑GGGCTGGCGAAGCGCCGGCCGGGGGTGAGGGATAGGGATGTACCA‑TTCCACGGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAAc                                            <  2:105888/228‑1 (MQ=255)                                                                                                                                                                                                        tC‑AAGACCGGC‑GTTT‑AACGCCGAGAAGTCGACTAA‑TTTGC‑TTTCCG‑GCGTGTCGA‑GGGCTTGCGCAACGTCGGCCTGCGGTGAGCGATAGCGATGTCCCA‑TTTCACGGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGtt                                       <  1:506583/239‑1 (MQ=255)                                                                                                                                                                                                         c‑AAGGCCGGC‑GGTA‑AACGCCGAGAAGCCGTCTAA‑TTTGT‑TTCCCA‑GGGTGTCGA‑GGGCTTGCGCAACCTCGGCGGGGGGGGGGCGAAAGCGATGGCCCA‑TTTCACTGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTa                                      <  2:101964/239‑1 (MQ=255)                                                                                                                                                                                                                cggc‑ggTT‑AACGCCGAGAAGTCGACTAA‑TTTTC‑TTTCCA‑GAGTGGCGA‑GGGCTTGCGCAACGTCGGGCCGCGGGGAGCGCTAGCGATGGACCA‑TTTCACTGATAACCGCCCGCAAAGGAAATTCCCAGAGGATGACTTCACGCCACCGGCCGCTTAAACGAATATCCAGCTTGCCCTTATCGTTGGCCACGGGGACCTGTTCCGGGGTAAAACGGGACTCGCGGAAACAGTTAAGATaa                                >  2:770658/1‑238 (MQ=255)                                                                                                                                                                                                                 ggc‑ggTT‑CACGCCGAGAAGTCGACTAA‑TTTTC‑TTTCCA‑GCGTGTCGG‑GGGCTTGCGCAACGTCGGCCTGCGGTGAGCGATAGCGATGTACCA‑TTTCTCTGATAAACCCCCGCAAAGGAACTTCCCCGAGGATGACTTCACGCCCCGGGGCGCTTAAACGAATATCCAGCTTGCCATTATTGTTGGACCCGGTGACTTTTTCCGCGTTAAAGCGGCCCACGCTGAACCAGCTAAAATat                                >  2:865326/1‑237 (MQ=255)                                                                                                                                                                                                                  gc‑ggTT‑AACCCCGAGAAATCGACTAA‑TTTTC‑TTTCCA‑GCGTGTCGA‑GGGCTTGCGCAACGTCGGCCTGCGGTGAGCGATAGCGATGGATCC‑TTTCACTGATAACCGCCAGCCAAGGAACTTCCCAGAGGGTGACTTCACGCCCCCGGCCGCCTAAACGAAAATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTc                              >  2:162345/1‑239 (MQ=255)                                                                                                                                                                                                                  gc‑ggTG‑ACCGGCGAGAAGTGGATAAT‑TTTGC‑TTTCCG‑GGGTGTCGA‑GGGTTGGCGAACCGCCGGCCTGGGGAGGGCGATAGCGATGACCCA‑TTTCACGGATAGCCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGt                               <  2:508199/238‑1 (MQ=255)                                                                                                                                                                                                                   ggggTT‑ACCGCCGAGAAGCCGACTAA‑TTTGC‑TTTCCA‑GCGTGTCGA‑GGGCTTGCGCAACGTCGGCCTGCGGTGAGCGATAGCGATGTACCA‑TTTCACTGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGt                               <  1:1083384/236‑1 (MQ=255)                                                                                                                                                                                                                        t‑AACGCCGAGAAGTCGTCTAT‑TTTGC‑TTTCCG‑GCGTGTCGG‑GGGTTTGCGAAACGTCGGCCTGCGGTGAGCGATAGCGATGTACCT‑TTTCACTGAAAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCt                         <  1:383357/239‑1 (MQ=255)                                                                                                                                                                                                                        t‑AACGCCGAGAAGTCGACTAA‑TTTGC‑TTTCCG‑GCGTGTCGA‑GGGCTTGCGCAACGTCGGCCTGCGGTGAGCGATAGCGATGTACCA‑TTTCACTGAAAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCt                         <  1:770658/239‑1 (MQ=255)                                                                                                                                                                                                                        t‑AACGCCAAGAAGCCGACTAA‑TTTGC‑TTTCCG‑GCGTGTCGA‑GGCCTTGCGCAACGTCGGCCTGCGGTGAGCGATAGCGATGTACCA‑TTTCACTGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCt                         <  1:860154/239‑1 (MQ=255)                                                                                                                                                                                                                         tagcgacGAGAAGTCTACTAA‑TTTGC‑TTTCCA‑GCGGTTCGA‑GGTCTTACGCACCGTCGGCCGGCGGTGAGGGATAGCGATGTACCA‑TTCCACTATTAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCt                         <  1:956524/233‑1 (MQ=255)                                                                                                                                                                                                                         taaggcgaagagGTCGACTAA‑GTTGC‑TTTCCT‑GCGTGTCGG‑GGGCTGGCGCAGCGGCTGCCGGGGGTGAGCAATAGGGATG‑ACCATTTTCACTAAAAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCt                         <  2:373052/231‑1 (MQ=255)                                                                                                                                                                                                                              ccGAGAAGTCGACTAA‑TTTGC‑TTTCCA‑GCGTGTCGA‑GGGCTTGCGCAACGTCGGCCTGCGGTGAGCGATAGCGATGTACCA‑TTTCACTGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCTTaaag                    >  2:261482/1‑239 (MQ=255)                                                                                                                                                                                                                                      ccGACTAT‑TTTGC‑TTCCAA‑GCGTGTGGA‑GGGCTGCCGCAACGTCGGCCTGCGGTGAGCGATAGCGATGTACCA‑TTTCACTGAAAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAata                                                                                                           <  2:1051242/143‑1 (MQ=255)                                                                                                                                                                                                                                       cGACTAA‑TTTGC‑TTTCCA‑‑CGGGTCGACGTGCGGGGCAAACGTCGGCCGGCGGTGCGGCATAGCGTTGGCCCA‑TTTCACTGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCTTAAAGAAAggca             <  2:553477/237‑1 (MQ=255)                                                                                                                                                                                                                                             a‑TTTGC‑TTCCCG‑GGGTGTCGA‑GGGCTTGCGCAACGTCGGCCTGCGGTGAGGGATAGCGATGTACCA‑TTTCACTGAAAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCTTAAAGAAAGGCAGGGCAGaa     <  1:756915/239‑1 (MQ=255)                                                                                                                                                                                                                                             a‑TTTGC‑TTCCCA‑GCGTGTCGG‑GGGCTTGCGCAACGTCGGCCTGCGGGGAGCGATAGCGATGTCCCA‑TTTCACTGATACCCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCTTAAAGAAAGGCAGGGCAGaa     <  1:31666/239‑1 (MQ=255)                                                                                                                                                                                                                                                 tGC‑TTTCCG‑GCGTGTGGG‑GGGCTTGCGCAACGTCGGCCTGGGGTGGCAGATAGCGATGTCCCA‑TTTCACTGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCTTAAAGAAAGGCAGGGCAGAAAGc  <  1:368407/239‑1 (MQ=255)                                                                                                                                                                                                                                                 tGC‑TTTCCA‑GCGTGTCGA‑GGGCTTGCGCAACGTCGGCCTGCGGTGAGCGATAGCGATGTACCA‑TTTCACTGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGc                                                                                                 >  1:1051242/1‑144 (MQ=255)                                                                                                                                                                                                                                                 tGC‑TTTCCA‑GCGTGTCGA‑GGGCTTGCGCAACGTCGGCCTGCGGTGAGCGATAGCGATGTACCA‑TTTCACTGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGACCTCGCGTAACCAGTTAAGATAGTCGGCCTTAAAGAAAGGCAGGGCAGAAAg   >  2:458893/1‑238 (MQ=255)                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                  GGGCTGATTTGCTGATGTGCCTGGAACCATTCGTGTGCCTGTGTTCCCATCGGCGTGAGGGAAAGCCGACGCGCCAGATCGTAGTTGCTGGTGCCCACAAACCAGGATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCGAC‑GGGTGCCAAAATCCATCAGATGGAAGCGCGACATATC‑AAGACCGGC‑GGTT‑AACGCCGAGAAGTCGACTAA‑TTTGC‑TTTCCA‑GCGTGTCGA‑GGGCTTGCGCAACGTCGGCCTGCGGTGAGCGATAGCGATGTACCA‑TTTCACTGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCTTAAAGAAAGGCAGGGCAGAAAGC  >  NC_000913/989665‑990138

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑