Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 989,945 A→G 19.6% S138P (TCA→CCA) ‡ pncB ← nicotinate phosphoribosyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913989,9450AG19.6% 131.1 / 4.3 79S138P (TCA→CCA) ‡pncBnicotinate phosphoribosyltransferase
Reads supporting (aligned to +/- strand):  ref base A (27/32);  new base G (6/11);  total (35/44)
Fisher's exact test for biased strand distribution p-value = 5.81e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TCCCATCGGCGTGAGGGAAAGCCGACGCGCCAGATCGTAGTTGCTGGTGCCCACAAACCAGGATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCGACGGGTGCCAAAATCCATCAGATGGAAGCGCGACATATCAAGACCGGCGGTTAACGCCGAGAAGTCGACTAATTTGCTTTCCAGCGTGTCGAGGGCTTGCGCAACGTCGGCCTGCGGTGAGCGATAGCGATGTACCATTTCACTGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCTTAAAGAAAGGCAGGGCAGAAAGCCACTGATATTCATCATCCTGCAGGCGCAGGTGCTGCATCGCCTGA  >  NC_000913/989709‑990183
                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                               
tCCCATCGGCGTGAGGGAAAGCCGACGCGCCAGATCGTAGTTGCTGGTGCCCACAAACCAGGATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCGACGGGTGCCAAAATCCATCAGATGGAAGCGCGACATATCAAGACCGGCGGTTAACGCCGAGAAGTCGACTAATTTGCTTTCCAGCGTGTCGAGGGCTTGCGCAACGTCGGCCTGCGGTGag                                                                                                                                                                                                                                                <  2:116440/238‑1 (MQ=255)
     tCGGCGTGAGGGAAAGCCGACGCGCCAGATCGTAGTTGCTGGTGCCCACAAACCAGGATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCGACGGGTGCCAAAATCCATCAGATGGAAGCGCGACATATCAAGACCGGCGGTTAACGCCGAGAAGTCGACTAATTTGCTTTCCAGCGTGTCGAGGGCTTGCGCAACGTCGGCCTGCGGTGagcgatag                                                                                                                                                                                                                                          <  1:900339/239‑1 (MQ=255)
       ggCGTGAGGGAAAGCCGACGCGCCAGATCGTAGTTGCTGGTGCCCACAAACCAGGATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCGACGGGTGCCAAAATCCATCAGATGGAAGCGCGACATATCAAGACCGGCGGTTAACGCCGAGAAGTCGACTAATTTGCTTTCCAGCGTGTCGAGGGCTTGCGCAACGTCGGCCTGCGGTGagcgatagcg                                                                                                                                                                                                                                        <  1:251466/239‑1 (MQ=255)
         cGTGAGGGAAAGCCGACGCGCCAGATCGTAGTTGCTGGTGCCCACAAACCAGGATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCGACGGGTGCCAAAATCCATCAGATGGAAGCGCGACATATCAAGACCGGCGGGTAAAGCCGAGAAAGCGGATAAATTGCTTTCCAGCGGGTCGAGGGGTTGCGCAAAGTCGGCCTGCGGGGggcgagagcggt                                                                                                                                                                                                                                      >  1:890454/1‑237 (MQ=255)
          gTGAGGGAAAGCCGACGCGCCAGATCGTAGTTGCTGGTGCCCACAAACCAGGATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCGACGGGTGCCAAAATCCATCAGATGGAAGCGCGACATATCAAGACCGGCGGTTAACGCCGAGAAGTCGACTAATTTGCTTTCCAGCGTGTCGAGGGCTTGCGCAACGTCGGCCTGCGGTGAGCGATAGCGATg                                                                                                                                                                                                                                     <  2:444842/239‑1 (MQ=255)
          gTGAGGGAAAGCCGACGCGCCAGATCGTAGTGGCTGGTGCCCACAAACCGGGATTCCTGTTGCAGACGCTTAACGATGGCTTCTTGTACCTCGCGAGAAAAACGGCGACGGGTGCCAAAATCCATCAGATGGAAGCGCGACATATCAAGACCGGCGGTTAACGCCGAGAAGTCGACTAATTTGCTTTCCAGCGTGTCGAGGGCTTGCGAAACGTCGGCCTGCGGTGAGCGATAGCGATg                                                                                                                                                                                                                                     <  2:420003/239‑1 (MQ=255)
           tGAGGGAAAGCCGACGCGCCAGATCGTAGTTGCTGGTGCCCACAAACCAGGATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCGACGGGTGCCAAAATCCATCAGATGGAAGCGCGACATATCAAGACCGGCGGTTAACGCCGAGAAGTCGACTAATTTGCTTTCCAGCGTGTCGAGGGCTTGCGCAACGTCGGCCTGCGGTGAGCGATAGCGATg                                                                                                                                                                                                                                     <  2:789900/238‑1 (MQ=255)
                   aGCCGACGCGCCAGATCGTAGTTGCTGGTGCCCACAAACCAGGATTCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCGACGGGTGCCAAAATCCATCAGATGGAAGCGCGACATATCAAGACCGGCGGTTAAAGCCCGGAAGGAGAATAATTTTGTTTTCCACGTGGCGGGGGGTTGTGCAAAGTCGGCCTGGGGGGAGCGTAAGCGGTGGACCCtttt                                                                                                                                                                                                                           >  1:1118632/1‑237 (MQ=255)
                        aCGCGCCAGATCGTAGTTGCTGGTGCCCACAAACCAGGATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCGACGGGTGCCAAAATCCATCAGATGGAAGCGCGACATATCAAGACCGGCGGTTAACGCCGCGAAGTCGAACAATTTGCTTTCCAGCGGGTGGAGGGCGTGCGCAACGTCCGCCTGCGGGGGGGGCTAGCCGGGTGCCATTTCCCttg                                                                                                                                                                                                                      >  2:1025507/1‑237 (MQ=255)
                         cgcgCCAGATCGTAGTTGCTGGTGCCCACAAACCAGGATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCGACGGGTGCCAAAATCCATCAGAGGGAAGCGCGACATATCAAGACCGGCGGTTAACGCCGAGAAGTCGACTAATTTGCTTTCCAGCGTGTCGAGGGCTTGCGCAACGTCGGCCTGCGGTGAGCGATAGCGATGTACCATTTCACTGa                                                                                                                                                                                                                      <  1:91303/238‑1 (MQ=255)
                           cgcCAGATCGTAGTTGCTGGTGCCCACAAACCAGGATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCGACGGGTGCCAAAATCCATCAGATGGAAGCGCGACATATCAAGACCGGCGGTTAACGCCGGGAAGTCGGCTCATTTTCTTTTCAGCGTGTCGGGGGGTTGCGGAAACGCGGGCTGCCGGGGGCGGCAGCGGTGTACCCTTTCTCTGATa                                                                                                                                                                                                                    >  2:1126734/1‑238 (MQ=255)
                                acgcGTAGTTGCGGGGCCCCACAAACCAGGATACCGTTGGCAGCCGTTTAACGATGGTTTTTTGTACTTCGCGAGAAAAACGGCGCCGGGGGCCAAAACCCATCAGATGGAAGCGCGACATACCAAGCCCGGCGGTTACCGCCGAGAAGTCGACTAATTTGCTTTCCAGCGTGGCGAGGGCTTGCGCAACTCCGGCCTGCGGTGAGCGATAGCGATGTACCATTTCACTGATAACCGcc                                                                                                                                                                                                              <  2:1036234/236‑1 (MQ=255)
                                      aGTTGCTGGTGCCCACAAACCAGGATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAACCGGCGACGGGTGCCAAAACCCATCAGATGGAAGCGCGACATATCAAGACCGGCGGTTAACGCCGAGAAGTCGACTAATTTGCTTTCCAGCGTGTCGAGGGCTTGCGCAACGTCGGCCTGCGGTGAGCGATAGCGATGTACCATTTCACTGATAACCGCCAGCaa                                                                                                                                                                                                         <  1:952787/238‑1 (MQ=255)
                                      aGTTGCTGGTGCCCACAAACCAGGATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCGACGGGTGCCAAAATCCATCAGATGGAAGCGCGACATATCAAGACCGGCGGTTAACGCCGAGAAGGCGGCTAATTTGCTTTCCAGCGTGTCGAGGGGTTGCGCCACGTCGGCCCGCGGGGAGCGATAGCGATGTACCATTTCACTGATAAACGCCCGCaaa                                                                                                                                                                                                        >  1:1005922/1‑239 (MQ=255)
                                             ggTGCCCACAAACCAGGATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCGACGGGTGCCAAAATCCATCAGATGGAAGCGCGACATATCAAGACCGGCGGTTAACGCCGAGAAGTCGACTAATTTGCTTTCCAGCGTGTCGAGGGCTTGCGCAACGTCGGCCTGCGGGGAGCGGTAGCGATGTACCCTTTCACTGATAACCGCCCGCAAAGGAACt                                                                                                                                                                                                  >  1:477396/1‑238 (MQ=255)
                                             ggTGCCCACAAACCAGGATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCGACGGGTGCCAAAATCCATCAGATGGAAGCGCGACATATCAAGACCGGCGGTTAAAGCCGAGAAGTCGACTAATTTGCTTTTCAGCGTGTCGAGGGCTTGCGCAACGTCGGCCTGCGGTGAGCGGTAGCGATGTAACATTTCACTGATAAACCCCCGCCAAGGaaat                                                                                                                                                                                                  >  2:1083384/1‑236 (MQ=255)
                                                                  cTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCGACGGGTGCCAAAATCCATCAGATGGAAGCGCGACATATCAAGACCGGCGGTTAACGCCGAGAAGTCGACTAATTTGCTTTCCCGCGTGTCGGGGGCGTGCGCAACGGCGGCCTGCGGTGGGCGATAGCGATGTACCATTTCACTGATAACCGCCCGCAAAGGAACATCCCAGAGGGTGAATTCACGc                                                                                                                                                                             >  1:1147389/1‑238 (MQ=255)
                                                                    gTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCGACGGGTGCCAAAATCCATCAGATGGAAGCGCGACATATCAAGACCGGCGGGTAAAGCCGCGAAGTCGACTAAATTGCTTTTCAGCGGGGCGGGGGCCTGCGCAAAGTCGGCCTTCGGTGTGCGGTAGCGGAGGACCAGTTTAATGATAAACCCCCGGCAAGGAACTTTCCCGGGGATGGATTTACGccc                                                                                                                                                                           >  2:563338/1‑237 (MQ=255)
                                                                      tgaaGACGGTTACGGAGGGTTTTTTGTACTGCGCGAAAAAACGGGCGAGGGGTGCAAAAATCCATCAGAGGGAAGCGCGACATACCAAGACCGGCGGTTAACGCCGAGAAGTCGACTAATTTGCTTCCCAGCGTGTGGAGGGCTTGCGCAACGTCGGCCTGGGGTGAGCGATAGCGATGTACCATTTCACTGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACg                                                                                                                                                                         <  2:696236/235‑1 (MQ=255)
                                                                       gCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCGACGGGTGCCAAAATCCATCAGATGGAAGCGCGACATATCAAGACCGGCGGTTAACGCCGAGAAGTCGACTAATTTGCTTTTCAGCGTGTCGGGGGGTTGCGCAACGACCGCCTGCCGGGGGCGGTAGCGCTGGAACATTTCACTCATAACCGCCCGCAAAGGCACTTCCCCGCGGGTGACCTctcccccccg                                                                                                                                                                        >  2:373855/1‑229 (MQ=255)
                                                                          gACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCGACGGGTGCCAAAATCCATCAGATGGAAGCGCGACATATCAAGACCGGCGGTTAACGCCCGGAAGTCGACTAATTTGCTTTCCAGCGGGTCGGGGGGTTGCGCCAAGGCGGCCCGCGGGGAGCGCAAGCGATGTAACCTTTCTCTGATAAACCCCAGCAAAGGGAATTCCCCGGGGATGACTTCACGCCCCGGGcc                                                                                                                                                                     >  2:383357/1‑238 (MQ=255)
                                                                          gACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCGACGGGTGCCAAAATCCATCAGATGGAAGCGCGACATATCAAGACCGGCGGTTAACGCCCAGAAGTCGACTAATTTTCTTTCCAGCGCGTCGGGGGCTTGCGCCACGTCGGCCCGCGGGGAGCGATAGCGATGTACCATTTCACTGATAACCGCCCGCAAAGGAACTTCCCCGAGGATGACTTCACGCCACGGGcc                                                                                                                                                                     >  1:590856/1‑238 (MQ=255)
                                                                                           tCTTGTACTTCGCGAGAAAAACGGCGACGGGTGCCAAAATCCATCAGATGGAAGCGCGACATATCAAGACCGGCGGTTAAAGCCGCGAAATCGCCTAATTTTCTTTCCAGCGTGTCGGGGGCTTGCGCCACCGCGGCCTGCGGGGAGCGGTAGCGGTGTAACATTTCACTGGTAACCCCCCCCCAAAGAACTTTCCCGAGGGTGAATTTTAGCCCCGGGCCCCTTTAAAGAatttaca                                                                                                                                                    >  2:378376/1‑233 (MQ=255)
                                                                                             tttgaCTGCGCGAAAAAAGCGGCTACGGGTCCCAATATCCAACAGAGGGAAGCGCGACAAATAGAGACCGGCGGTTAACGCCGAGAGGTCGACTAATTTGCTTCCCAGCGGGTCGAGGGTTTGCGCAATTTCGGCCTGCGGTGAGCGATGAAGTTGTACAATTTCCCTGAAACCCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCt                                                                                                                                                 <  2:800021/235‑1 (MQ=255)
                                                                                                   ttCGCGAGAAAAACGGCGAGGGGTGCCAAAATCCATCAGAGGGAAGTGGAAATACCAAAGACCCGGGGTTAGCGCAGGAAGGTCCATTATTTTTTTTCCACGGTTCTCGAGGGCTTGCGAAACTTCGGCTTGGGGGGGGGGATGGGAATGTACCATTTCACTGAAAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCAt                                                                                                                                           <  2:418862/239‑1 (MQ=255)
                                                                                                   tgcgcgAGAAAACCGGGGGCGGGGGAAAAAACCAAACGAATGGAAGCGGGACATATCAGGCCGGGCGGTTACCGCCGAGAAGCCGACTAATTTGCTTTCCAGCGTGTGGAGGGTTTGCGCAACGTCGGCCGGGGGTGAGCGAGAGCGATGTACCATTTCACTGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCAt                                                                                                                                           <  2:259729/237‑1 (MQ=255)
                                                                                                     ggcgAGAAAACGGCGGCGGGGGGCCAAAAGCCATCAGACGAAAGCGCGACATTTCAAGCCCGGCGGTAAACGCCGAGAAGCCGCTTATTTTGCTTCCAAGCGTGTGGAGGCCTTGCGCAACGTCGGCGTGCGGTGAGCGATAGGGATGTACCTTTTCCCTGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTa                                                                                                                                         <  2:606028/238‑1 (MQ=255)
                                                                                                     cgcgAGAAAAACGGCGACGGGTGCCAAAATCCATCAGATGGAAGCGCGACATATCAAGACCGGCGGTTAACGCCGCGAAGTCGACTAATTTTCTTTCCAGCGTGTCGAGGGCTTGCGCAACGTCGGCCTGCGGTGAGCGGTAGCGATGTACCATTTCACTGATAACCCCCCGCAAAGGAACTTCCCAGAGGGTGACTTCACGCCACGGGGCGCCTAAAAGGAAATCCAACTTGCCAttt                                                                                                                                         >  1:1103764/1‑238 (MQ=255)
                                                                                                     cgcgAGAAAAACGGCGACGGGTGCCAAAATCCATCAGATGGAAGCGCGACATATCAAGACCGGCGGTTAACGCCCAGAAGTCGACTAATTTTCTTTTCAGCCGGTCGAGGGCTTGCGCAACCACGGCCTGCGGGGAGCGATAGCGGTGTACCATTTTACTTATAAACCCCCGCAAAAGAAATTCCCACAGGAAGACTTCACCCCACCGGCCGACTAAAAGAATATCCAACCTGTCATTa                                                                                                                                         >  2:737493/1‑239 (MQ=255)
                                                                                                     cgcgAGAAAAACGGCGACGGGTGCCAAAATCCATCAGATGGAAGCGCGACATATCAAGACCGGCGGTTAAAGCCGAGAAGTCGACTAATTTGCTTTTCAGCGTGTCCAGGGCTTGGGAAACATCGGCCTGCGGTGAGAGCTAAGGCTGTTCCATTTCACTTAATAACGCCACACAAGAAACATCCCCGCGGGTGACCTCAACCCCCGCGGCCCTTAAACGGAAATCCaacctcacctta                                                                                                                                         >  2:742550/1‑228 (MQ=255)
                                                                                                     cgcgAGAAAAACGGCGACGGGTGCCAAAATCCATCAGATGGAAGCGCGACATATCAAGACCGGCGGGTAACACCGAAAAGTAGACCAATTTGCTTTTCAACGTGGTGAAGGCTTTCGCAAAGTCGGGCTGCGGTTCGGGGTAGCGCGGAACCAGCTCATCGATTACCGCCACCAAACGAAGTTCCCCGAGGATGACGTCACTCCACGGCACCCTTAAAAGAATATCCAGCTTGGCAtgt                                                                                                                                         >  2:699412/1‑237 (MQ=255)
                                                                                                     cgcgAGAAAAACGGCGACGGGTGCCAAAATCCATCAGATGGAAGCGCGACATATCAAGACCGGCGGCTAACGCCGAGAAGTCGACTAATTTGCTTTCCAGCCTGTCGAGGGGTTTCGCAAAGTCGGCCTGCGGGGGGCGCTAGCGATGTAACATTTCACTGATAACCCCCAGCAAAGGAACTTCCCAGAGGATGACTTTACCCCCCGGGCCGCCTAAAAGAATATCCAGCTTGGCATTa                                                                                                                                         >  2:125872/1‑239 (MQ=255)
                                                                                                       cgAGAAAAACGGCGACGGGTGCCAAAATCCATCAGATGGAAGCGCGACATATCAAGACCGGCGGTTAACGCCGAGAAGTCGACTAATTTGCTTTCCAGCGTGTCGAGGGCTTGCGCAACGTCGGCCCGCGGTGAGCGATAGCGATGTACCATTTCACTGTTAACCGCCCGCAAAGGAACTTCCCAGAGGGTGACTTCACCCCACGGGCCGCCTAAAAGAAAAATCAGCTTTCCATTATc                                                                                                                                       >  1:105888/1‑239 (MQ=255)
                                                                                                           aaaaaCGGCGGGGGGTCGCAAACCCCACAAGATGGAACGGCGACATACAAAAGCGGGCGGTTAACGCAGAGAAGTCGCTTATTTGCTTTTCCAGCGTGTGGAGGATTTGGCCAAGGTCGGCCTGGGGTGGGCGAAAGGGATGTCCCATTTCATGGAGAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTg                                                                                                                                   <  2:136731/239‑1 (MQ=255)
                                                                                                                                         agagGGAAGGGGGACATAACAAGACGGGGGGTAACCGCGGAGAAGCCGACTATTTTGCTTCCAGGGGGGGCGGGGGCTGGCGAAACGTCGGCCGGCGGGGGGCGATGGCGATGTCCCATTCCACTGATACCCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAg                                                                                                        <  2:590856/237‑1 (MQ=255)
                                                                                                                                             ggAAGCGCGACATATCAAGACCGGCGGTTAACGCCGAGAAGGCGACTAATTTGCTTTCCAGCGTGTCGAGGGCGTGCGCAACGTCGGCCTGCGGTGAGCGATAGCGATGTACCATTTCACTGATAAACACCCGCAAAAGAACTTCCCAAAGGGAGACTTCACGCCCCCGGGCGCCTAAAAGAAAATCCACCTCGCCATTATCGTTTGTCACGGTGACTTGTTTCGGGTTTAAGCGGaa                                                                                                   >  2:79422/1‑238 (MQ=255)
                                                                                                                                               aaGCGCGACATATCAAGACCGGCGGTTAAAGCCGAGAAGTCGACTAATTTTCTTTCCAGCGTGTCGAGGGCTTGCGCAAAGTCGGCCCGCGGGGGGCGAAAGCGATGTACCATTTCACTGATAACCGCCAACAAAGGAACTTCCCAGAGGGTGACTTCACGCCCCCGGCCGCTTCAACGAAAATCCCACTTTCCATTTTCGTTTGACACGGTGACTTGGTCCGGGGTAAAGCGGaaacc                                                                                                >  2:48225/1‑236 (MQ=255)
                                                                                                                                                    cgACATATCAAGACCGGCGGTTAAAGCCGAGAAGTCGACTAATTTTCTTTTCCGCGTGTCGAGGGCTTGCGCAACGTCGGCCTGCGGGGAGCGATAGCGATGTACCATTTTACTGATAACCGCCCGCAAAAGAAATTCCCAGAGGGTGACTTCCCGCCACGGGCCCCTTAAAAGAATATCCAGCTTTCCATTATCGTTGGACACGGTGACTTGTTCCGGGGTTAAGCGGAACTCGCGTa                                                                                           >  1:822188/1‑239 (MQ=255)
                                                                                                                                                     gacaagtCAAGACCGGGGGATAACGCCGAGAAGTCGACGATTTTGCTTTCCGGGGGGTCGAGGGCTTGCGCAACGCCGGCCTGCGGTGGGTGATAGCGATGTACCATTTCACTGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTaa                                                                                          <  2:671591/233‑1 (MQ=255)
                                                                                                                                                     gACATTACAAGCCGGGGGGTTATCGCCGAGAGGTCGGCTAATTGTCTTGCCGTCTGTTCGGGGGCTGGCAAAACGCCGCCGGGGGGGGGGCGATAGCGTGGTACCATTCCTCTGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTaa                                                                                          <  2:1118632/239‑1 (MQ=255)
                                                                                                                                                     gACATATCAGGCCCGGCGGTTAACGCCGAGAAGTCGACTAATTTGCTTTCCAGCGTGTCGAGGGCTGGCGCAACGTCGGCCTGCGGTGGGCGATAGCGATGTCCCTTTTCACGGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTaa                                                                                          <  1:48225/239‑1 (MQ=255)
                                                                                                                                                      atatatAAAGACCGGCGCTCACGGGCGAGAAGTCGCCTAATTTGCTCTCCAGCGTTTCGAGGGCTCGCGCAAGGTGGGCCGGCGGTGAGCCAAGGCGATGTACTATTTCACTGAGCGCCGCCAGCAAAGGAACTTCCCAGAGGATGACTGCGCGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTaa                                                                                          <  2:608915/236‑1 (MQ=255)
                                                                                                                                                       acagtaaaggcccGGGGTTTCACGCCGAGAAGCCACTTATTTTTCTTCCCTGCGTGGCGTGGGTTTGCGAAACGGCGGCCTGGGGTGAGTGAATGCGTGTTACCATTTAACTGAGACCCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTaa                                                                                          <  2:128173/227‑1 (MQ=255)
                                                                                                                                                       aaatacgaaGACGGGCGGTTAGCGCCGAGAAGTCGGCTATTTTGCTTCCCGGCGTGGCGAGGTCTTGCGAAACGCCGGCCTGGGGTGAGCGATAGCGAGTTACCATTTCACTAATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTaa                                                                                          <  2:1147389/231‑1 (MQ=255)
                                                                                                                                                        aatagcagacccGGAGTTTAACGCCGAAAAGTCGTCTATTTTGCTTTCCGGCGTGTCGAGGGCTGGCGAAGCGCCGGCCGGGGGTGAGGGATAGGGATGTACCATTCCACGGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAAc                                                                                         <  2:105888/228‑1 (MQ=255)
                                                                                                                                                           tCAAGACCGGCGTTTAACGCCGAGAAGTCGACTAATTTGCTTTCCGGCGTGTCGAGGGCTTGCGCAACGTCGGCCTGCGGTGAGCGATAGCGATGTCCCATTTCACGGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGtt                                                                                    <  1:506583/239‑1 (MQ=255)
                                                                                                                                                            cAAGGCCGGCGGTAAACGCCGAGAAGCCGTCTAATTTGTTTCCCAGGGTGTCGAGGGCTTGCGCAACCTCGGCGGGGGGGGGGCGAAAGCGATGGCCCATTTCACTGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTa                                                                                   <  2:101964/239‑1 (MQ=255)
                                                                                                                                                                   cggcggTTAACGCCGAGAAGTCGACTAATTTTCTTTCCAGAGTGGCGAGGGCTTGCGCAACGTCGGGCCGCGGGGAGCGCTAGCGATGGACCATTTCACTGATAACCGCCCGCAAAGGAAATTCCCAGAGGATGACTTCACGCCACCGGCCGCTTAAACGAATATCCAGCTTGCCCTTATCGTTGGCCACGGGGACCTGTTCCGGGGTAAAACGGGACTCGCGGAAACAGTTAAGATaa                                                                             >  2:770658/1‑238 (MQ=255)
                                                                                                                                                                    ggcggTTCACGCCGAGAAGTCGACTAATTTTCTTTCCAGCGTGTCGGGGGCTTGCGCAACGTCGGCCTGCGGTGAGCGATAGCGATGTACCATTTCTCTGATAAACCCCCGCAAAGGAACTTCCCCGAGGATGACTTCACGCCCCGGGGCGCTTAAACGAATATCCAGCTTGCCATTATTGTTGGACCCGGTGACTTTTTCCGCGTTAAAGCGGCCCACGCTGAACCAGCTAAAATat                                                                             >  2:865326/1‑237 (MQ=255)
                                                                                                                                                                     ggggTTACCGCCGAGAAGCCGACTAATTTGCTTTCCAGCGTGTCGAGGGCTTGCGCAACGTCGGCCTGCGGTGAGCGATAGCGATGTACCATTTCACTGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGt                                                                            <  1:1083384/236‑1 (MQ=255)
                                                                                                                                                                     gcggTTAACCCCGAGAAATCGACTAATTTTCTTTCCAGCGTGTCGAGGGCTTGCGCAACGTCGGCCTGCGGTGAGCGATAGCGATGGATCCTTTCACTGATAACCGCCAGCCAAGGAACTTCCCAGAGGGTGACTTCACGCCCCCGGCCGCCTAAACGAAAATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTc                                                                           >  2:162345/1‑239 (MQ=255)
                                                                                                                                                                     gcggTGACCGGCGAGAAGTGGATAATTTTGCTTTCCGGGGTGTCGAGGGTTGGCGAACCGCCGGCCTGGGGAGGGCGATAGCGATGACCCATTTCACGGATAGCCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGt                                                                            <  2:508199/238‑1 (MQ=255)
                                                                                                                                                                          tagcgacGAGAAGTCTACTAATTTGCTTTCCAGCGGTTCGAGGTCTTACGCACCGTCGGCCGGCGGTGAGGGATAGCGATGTACCATTCCACTATTAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCt                                                                      <  1:956524/233‑1 (MQ=255)
                                                                                                                                                                          taaggcgaagagGTCGACTAAGTTGCTTTCCTGCGTGTCGGGGGCTGGCGCAGCGGCTGCCGGGGGTGAGCAATAGGGATGACCATTTTCACTAAAAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCt                                                                      <  2:373052/231‑1 (MQ=255)
                                                                                                                                                                          tAACGCCGAGAAGTCGTCTATTTTGCTTTCCGGCGTGTCGGGGGTTTGCGAAACGTCGGCCTGCGGTGAGCGATAGCGATGTACCTTTTCACTGAAAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCt                                                                      <  1:383357/239‑1 (MQ=255)
                                                                                                                                                                          tAACGCCGAGAAGTCGACTAATTTGCTTTCCGGCGTGTCGAGGGCTTGCGCAACGTCGGCCTGCGGTGAGCGATAGCGATGTACCATTTCACTGAAAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCt                                                                      <  1:770658/239‑1 (MQ=255)
                                                                                                                                                                          tAACGCCAAGAAGCCGACTAATTTGCTTTCCGGCGTGTCGAGGCCTTGCGCAACGTCGGCCTGCGGTGAGCGATAGCGATGTACCATTTCACTGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCt                                                                      <  1:860154/239‑1 (MQ=255)
                                                                                                                                                                               ccGAGAAGTCGACTAATTTGCTTTCCAGCGTGTCGAGGGCTTGCGCAACGTCGGCCTGCGGTGAGCGATAGCGATGTACCATTTCACTGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCTTaaag                                                                 >  2:261482/1‑239 (MQ=255)
                                                                                                                                                                                       ccGACTATTTTGCTTCCAAGCGTGTGGAGGGCTGCCGCAACGTCGGCCTGCGGTGAGCGATAGCGATGTACCATTTCACTGAAAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAata                                                                                                                                                        <  2:1051242/143‑1 (MQ=255)
                                                                                                                                                                                        cGACTAATTTGCTTTCCACGGGTCGACGTGCGGGGCAAACGTCGGCCGGCGGTGCGGCATAGCGTTGGCCCATTTCACTGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCTTAAAGAAAggca                                                          <  2:553477/237‑1 (MQ=255)
                                                                                                                                                                                              aTTTGCTTCCCGGGGTGTCGAGGGCTTGCGCAACGTCGGCCTGCGGTGAGGGATAGCGATGTACCATTTCACTGAAAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCTTAAAGAAAGGCAGGGCAGaa                                                  <  1:756915/239‑1 (MQ=255)
                                                                                                                                                                                              aTTTGCTTCCCAGCGTGTCGGGGGCTTGCGCAACGTCGGCCTGCGGGGAGCGATAGCGATGTCCCATTTCACTGATACCCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCTTAAAGAAAGGCAGGGCAGaa                                                  <  1:31666/239‑1 (MQ=255)
                                                                                                                                                                                                  tGCTTTCCGGCGTGTGGGGGGCTTGCGCAACGTCGGCCTGGGGTGGCAGATAGCGATGTCCCATTTCACTGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCTTAAAGAAAGGCAGGGCAGAAAGc                                               <  1:368407/239‑1 (MQ=255)
                                                                                                                                                                                                  tGCTTTCCAGCGTGTCGAGGGCTTGCGCAACGTCGGCCTGCGGTGAGCGATAGCGATGTACCATTTCACTGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGc                                                                                                                                              >  1:1051242/1‑144 (MQ=255)
                                                                                                                                                                                                  tGCTTTCCAGCGTGTCGAGGGCTTGCGCAACGTCGGCCTGCGGTGAGCGATAGCGATGTACCATTTCACTGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGACCTCGCGTAACCAGTTAAGATAGTCGGCCTTAAAGAAAGGCAGGGCAGAAAg                                                >  2:458893/1‑238 (MQ=255)
                                                                                                                                                                                                    cTTTCCAGCGTGTCGAGGGCTTGCGCAACGTCGGCCTGCGGTGAGCGATAGCGATGTACCATTTCACTGATAACCGCCAGCAAAGGAACTTCCCa                                                                                                                                                                                             >  2:168276/1‑95 (MQ=255)
                                                                                                                                                                                                    cTTTCCAGCGTGTCGAGGGCTTGCGCAACGTCGGCCGGCGGTGAGCGATAGCGATGTACCATTTCACTGATAACCGCCAGCAAAGGAACTTCCCa                                                                                                                                                                                             <  1:168276/95‑1 (MQ=255)
                                                                                                                                                                                                      ttcccAGCGTGTGGGGGGCTTGCGCAACGCCGGCCGGCGGTGAGCGATAGCGATGTACCATTTCCCTGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCTTAAAGAAAGGCAGGGCAGAAAGCCAc                                            <  1:1025507/236‑1 (MQ=255)
                                                                                                                                                                                                       tttcaggCGTGTCGAAGGCTGGCGCACGGTCGGCCTGCGGGGAGCGATGGGGATGTCCCATTTCACTGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCTTAAAGAAAGGCAGGGCAGAAAGCCAc                                            <  1:499859/233‑1 (MQ=255)
                                                                                                                                                                                                        tCCAGCGTGTCGAGGGCTTGCGCAACGTCGGCCTGCGGTGAGCGATAGCGATGTACCATTTCACTGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCTTAAAGAAAGGCAGGGCAGAAAGCCACTg                                          >  1:635268/1‑239 (MQ=255)
                                                                                                                                                                                                        tCCAGCGTGTCGAGGGCTTGCCCAACGTGGGCTTGCGGTGGGCGATAGCGATGTCCCATTTCACTGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCTTAAAGAAAGGCAGGGCAGAAAGCCACTg                                          <  1:125872/239‑1 (MQ=255)
                                                                                                                                                                                                                             gcgcAACGTCGGCCTGCGGTGAGCGATAGCGATGTACCATTTCACTGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAAc                                                                                         >  1:736279/1‑173 (MQ=255)
                                                                                                                                                                                                                             gcgcAACGTCGGCCTGCGGTGAGCGATAGCGATGTACCATTTCACTGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCTTAAAGAAAGGCAGGGCAGAAAGCCACTGATATTCATCATCCTGCAg                        >  1:232185/1‑238 (MQ=255)
                                                                                                                                                                                                                             gcgaAACGTCGGCCGGGGGGGAGCGAGAGCGATGTCCCATTTCACTGAAAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCTTAAAGAAAGGCAGGGCAGAAAGCCACTGATATTCATCATCCTGCAgg                       <  2:822188/239‑1 (MQ=255)
                                                                                                                                                                                                                              cgcAACGTCGGCCTGCGGTGAGCGATAGCGATGTACCATTTCACTGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCTTAAAGAAAGGCAGGGCAGAAAGCCACTGATATTCATCATCCTGCAGgc                      >  1:1188491/1‑239 (MQ=255)
                                                                                                                                                                                                                               gcAACGTGGGCCTGCGGTGAGCAATAGCAATGTCCCCTTTCACTGAAAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCTTAAAGAAAGGCAGGGCAGAAAGCCACTGATATTCATCATCCTGCAGgcg                     <  1:733799/239‑1 (MQ=255)
                                                                                                                                                                                                                                 atgtccGGCCTGCGGTGAGCAAAAGCGTGTTCCCATTCCACTGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCTTAAAGAAAGGCAGGGCAGAAAGCCACTGATATTCATCATCCTGCAGgcgc                    <  1:1225322/233‑1 (MQ=255)
                                                                                                                                                                                                                                 aaCGTCGCCCTGCGGTGAGCGATAGCGATGTCCCATTCCACTGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCTTAAAGAAAGGCAGGGCAGAAAGCCACTGATATTCATCATCCTGCAGgcgc                    <  1:584196/238‑1 (MQ=255)
                                                                                                                                                                                                                                          cTGCGGTGGGGGATAGCGATGTCCCATTTCACTGAAAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCTTAAAGAAAGGCAGGGCAGAAAGCCACTGATATTCATCATCCTGCAGGCGCAGGTGCTGCa          <  2:1221535/239‑1 (MQ=255)
                                                                                                                                                                                                                                          cTGCGGTGAGCGATGGCGATGTACCATTTCACGAATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCTTAAAGAAAGGCAGGGCAGAAAGCCACTGATATTCATCATCCTGCAGGCGCAGGTGCTGCa          <  1:373855/239‑1 (MQ=255)
                                                                                                                                                                                                                                             cGGTGAGCGATAGCGATGTACCATTTCACTGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCTTAAAGAAAGGCAGGGCAGAAAGCCACTGATATTCATCATCCTGCAGGCGCAGGTGCTGCATc        >  2:25405/1‑238 (MQ=255)
                                                                                                                                                                                                                                             cGGTGAGCGATAGCGATGTACCATTTCACTGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCTTAAAGAAAGGCAGGGCAGAAAGCCACTGATATTCATCATCCTGCAGGCGCAGGTGCTGCATc        >  2:934389/1‑238 (MQ=255)
                                                                                                                                                                                                                                               gTGAGCGATAGCGATGTACCATTTCACTGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCTTAAAGAAAGGCAGGGCAGAAAGCCACTGATATTCATCATCCTGCAGGCGCAGGTGCTGCATCg       >  1:825185/1‑237 (MQ=255)
                                                                                                                                                                                                                                                  agcgatagcgatGTACCATTTCACTGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCTTAAAGAAAGGCAGGGCAGAAAGCCACTGATATTCATCATCCTGCAGGCGCAGGTGCTGCATCGCCTGa  >  2:598059/1‑239 (MQ=255)
                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                               
TCCCATCGGCGTGAGGGAAAGCCGACGCGCCAGATCGTAGTTGCTGGTGCCCACAAACCAGGATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCGACGGGTGCCAAAATCCATCAGATGGAAGCGCGACATATCAAGACCGGCGGTTAACGCCGAGAAGTCGACTAATTTGCTTTCCAGCGTGTCGAGGGCTTGCGCAACGTCGGCCTGCGGTGAGCGATAGCGATGTACCATTTCACTGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCTTAAAGAAAGGCAGGGCAGAAAGCCACTGATATTCATCATCCTGCAGGCGCAGGTGCTGCATCGCCTGA  >  NC_000913/989709‑990183

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: