Predicted mutation | |||||||
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evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | NC_000913 | 2,151,689 | G→T | 38.6% | intergenic (+43/+22) | yegJ → / ← yegK | uncharacterized protein/ser/thr phosphatase‑related protein |
Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 2,151,689 | 0 | G | T | 38.6% | 13.4 / 10.0 | 34 | intergenic (+43/+22) | yegJ/yegK | uncharacterized protein/ser/thr phosphatase‑related protein |
Reads supporting (aligned to +/- strand): ref base G (14/6); new base T (6/7); total (20/14) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 2.76e-01 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.64e-01 |
TGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTATACC‑ATTGATCCTTTATTGGTCA‑CCTATCCAAAAGAAGAAGCCGACGAGC‑TAAGAGCAAAATTAGTTCGCTAA‑TCAGCC‑GTTATA‑TTTATTGTTTAAACTGGCGGTTGAATTGTTGTC‑TT‑CAA‑C‑CGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGCATATTTAACGCCAGTCGCTGGATACCATCCGTAAATG > NC_000913/2151496‑2151925 | tGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTTTAAC‑ATTTATTCCTTTTTTGTCACCCAATCCAAAA‑AAGAAAGCGACGAGC‑TAAAAGGAAAAATAATTTGCTTA‑TCAACC‑GGTTTATTTTTTTTTTTAAATTGGGGGTTTGATTTTTTTT‑TT‑TAA‑CACCCCGTAAAAAACACACTTTCCCcacacccagcg > 1:477713/1‑231 (MQ=255) aaaaCAAAAAAGAAGATATTTCAGCCTGGATGTATACGCGGGGGGATAAAATTTACGGCGTTTATCCC‑ATTGATCCTTTATTGGTCC‑CCTACCCAAAAGAAGAAGCCGACGAGC‑TAAGAGAAAAATTAGTTCGCTAA‑TCACCC‑GTTATA‑TTTATTGTTTAAACTGGCGTTTGATTTGTTGTC‑TT‑CAA‑C‑CGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTatcat < 1:436470/239‑1 (MQ=255) aaaaaaaaGATATTTCAGCTGGGATTTATGCGGGGGGGAAAAAAATTACCGGGGTTTATACC‑ATTACTCTTTTTTGGTTCC‑CCTAACAAAAAGAAAAAGCCGAGGGGA‑AAAGAAAAAAATTTGTTCGCT‑A‑TCCGCCTTTTATA‑TTTTTTGTTAAAACGGGGGTTTAATTTGTTGTT‑TC‑AAA‑C‑CGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCg < 2:1077744/237‑1 (MQ=255) cagatggtttGTATCCGCGGGGGGATAAAATTTGCGGCGTTTATACC‑ATTTATCTTTTATTGTTCC‑CCTATCAAAAAGAAGAAGCCGACGAGA‑TAAGAGAAAATTTAGTCCGTAAC‑TCAGCC‑TTTATT‑TTTTTTTTTTAACCGGGCGTTTGATTTTTTTTC‑TA‑CAA‑C‑CGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTAccg < 2:477713/230‑1 (MQ=255) cgcgggGGAAAAAAATTAAGGGGGTTTATACA‑ATTGACCTTTTATGGTCCC‑CCTACCAAAAAAAAGAAGCCGAGGAGC‑TAAGAG‑AAAATTTATTCCCTAATTCAGCC‑TTTATT‑TTTTTTTTTAAAACGGGGGGTTAATTTTTTGTC‑TC‑CAA‑C‑CGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACaaaa < 1:653900/239‑1 (MQ=255) ttACGG‑GGTTTTACCAATTGTCCTTTTATTGGCCC‑CCTA‑CCAAAAAAAAGAGCCCAAGAACATAAAACAAAATTTAGTCCGTAAA‑TCAGCT‑TTTTTA‑TTTTTTTTTAAACCGGGGGGTTAATTTTTTTTC‑TC‑CAA‑C‑CGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTc < 1:614736/239‑1 (MQ=255) tGATCCTTTATTGGTCA‑CCTATCCAAAAGAAGAAGCCGACGAGC‑TAAGAGCAAAATTAGTTCGCTAA‑TCAGCC‑GTTATA‑TTTATTGTTTAAACTGGCGGTTGAATTGTTGTC‑TT‑CAA‑C‑CGCCCTGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTTTTATCATCCGTACGCCCGGTTACCCCCGGGCTGGACAAAAACTGCTTTAACAATTCAg > 1:463056/1‑223 (MQ=255) tGATCCTTTATTGGTCA‑CCTATCCAAAAGAAGAAGCCGACGAGC‑TAAGAGCAAAATTAGTTCGCTAA‑TCAGCC‑GTTATA‑TTTATTGTTTAAACTGGCGGTTGAATTGTTGTC‑TT‑CAA‑C‑CGCCATGAGGAACTCAATCTGCCCACAGAGCCAGTGCCCACGGTTTATCATCATCCGTACGGCCGTTTCACGCCGGGCTGGACAAAAACTGCTTTAACAAATCAGGTAATAAAACGAggtt > 1:793499/1‑236 (MQ=255) tGATCCTTTATTGGTCA‑CCTATCCAAAAGAAGAAGCCGACGAGC‑TAAGAGCAAAATTAGTTCGCTAA‑TCAGCC‑GTTATA‑TTTATTGTTTAAACTGGCGGTTGAATTGTTGTC‑TT‑CAA‑C‑CGCCATGAGAAACTCAATCTTCCCACAGCGCCCGTGCCAACGGTTTTTCATCCTCCGTCCGCTCGTTTACCGCCGGGCTGGACAAAAACTGCCTTAAAAATTCAGGGAAAAAAACGAgttg > 1:895467/1‑239 (MQ=255) gATCCTTTATTGGTCA‑CCTATCCAAAAGAAGAAGCCGACGAGC‑TAAGAGCAAAATTAGTTCGCTAA‑TCAGCC‑GTTATA‑TTTATTGTTTAAACTGGCGGTTGAATTGTTTTC‑TT‑CAA‑C‑CGCCATGAGAAACTCACTCTGCCCACAGCGCCAGGGCCAACGGTTTATCATCATCCGTACGCTCGCTTACCCCCGGGCTGGACAAAAAACGCTTTAACAATTCAGGGAATAAATCGAgttgt > 2:1070438/1‑239 (MQ=255) ccTTTATTGGTCA‑CCTATCCAAAAGAAGAAGCCGACGAGC‑TAAGAGCAAAATTAGTTCGCTAA‑TCAGCC‑GTTATA‑TTTATTGTTTAAACTGGCGGTTGAAATGTTGTT‑TT‑CAA‑C‑CGCCAAGAGAAACTCAATCTGCCCACAGCGCCAGTGCCAACGGTTTTTCATCATCCGTACGCTCGTTTAACGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAgttgtt > 1:1048379/1‑237 (MQ=255) ttATTGGTCA‑CCTATCCAAAAGAAGAAGCCGACGAGC‑TAAGAGCAAAATTAGTTCGCTAA‑TCAGCC‑GTTATA‑TTTATTGTTTAAACTGGCGGTTGAATTTTTTTC‑TT‑CCA‑C‑CGCCATTAGAAAATCACTCTGCCCACAGCGCCAGTGCCAACCGTTTTTCCTTCTCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAAACGCTTTAACAATTTAGGGAAAAAATAGAattttttccgc > 2:604140/1‑234 (MQ=255) ttATTGGTCA‑CCTATCCAAAAGAAGAAGCCGACGAGC‑TAAGAGCAAAATTAGTTCGCTAA‑TCAGCC‑GTTATA‑TTTATTGTTTAAACTGGCGGTTGAATTGTTGTC‑TT‑CAA‑C‑CGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGGTGTTCCTGc > 1:801298/1‑239 (MQ=255) a‑CCTATCCAAAAGAAGAAGCCGACGAGC‑TAAGAGCAAAATTAGTTCGCTAA‑TCAGCC‑GTTATA‑TTTATTGTTTAAACTGGCGGTTGAATTGTTTTT‑TT‑CAA‑C‑CGCCATGAGAAACTCACTCTTCCCACAGCGCCAGTGCCACAGGTTTTTCATCATCCGCACGCTCGTTTACCGCCGGGCTGGGCCAAAACCGGTTTTACCATTCAGGTAATTAATCGAGTTGGTCCTGCGTttctgc > 2:1158122/1‑238 (MQ=255) a‑CCTATCCAAAAGAAGAAGCCGACGAGC‑TAAGAGCAAAATTAGTTCGCTAA‑TCAGCC‑GTTATA‑TTTATTGTTTAAACTGGCGGTTGAATTGTTGTC‑TT‑CAA‑C‑CGCCATGAGAAAATCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTtgctgc > 1:492121/1‑238 (MQ=255) aTCCAAAAGAAGAAGCCGACGAGC‑TAAGAGCAAAATTAGTTCGCTAA‑TCAGCC‑GTTATA‑TTTATTGTTTAAACTGGCGGTTGTATTTTTTCC‑TT‑CAA‑C‑CGCCATTAGAAACTCACTCTGCCCCCAGCGCCACGTCCAACCGTTTTTTATCAACCGTACGCCCGCTTTCCGCCCGGCTGGACCAAAAACCCTTTTACAAATCAGGTAATAAATCGAGTTGTTCCTCCGTTGCTgtcgccc > 2:502177/1‑238 (MQ=255) gaagCCGACGAGC‑TAAGAGCAAAATTAGTTCGCTAA‑TCAGCC‑GTTATA‑TTTATTGTTTAAACTGGCGGTTTAATTGTTGAC‑TT‑TAA‑C‑CGCCATGAGGAAATTACTCTGCCCACAGGGTCAGTGCCAACGTTTTATCATCATCCGTTCGCTCGTTTACCGCCGGGGTGGACAAAAAATGCTTTTACAATTCATGGACTAAATCGAGTTTTTCCTGCGGTGCTTGCTCCCGTCCATTGata > 2:252931/1‑237 (MQ=255) aagCCGACGAGC‑TAAGAGCAAAATTAGTTCGCTAA‑TCAGCC‑GTTATA‑TTTATTGTTTAAACTGGCGGTTGAATTTTTTTC‑TT‑TAA‑A‑CGCCATGAGAAACTCACTCTGCCCACAACGCCAGTGCCAACGTTTTATTATCATCCGCAAGCCCGTTTACCCCCGGGCTGGACACAAAATGCATTATAAATTTAGGGAATAAAACGAGTTGTTTCTGCGTTGCTGCCGCCAGTCCAtttcaaa > 2:808528/1‑234 (MQ=255) agCCGACGAGT‑AAAAAAAAAATTTAGTCCTTTAA‑CCACCC‑TTTATT‑TTTTTTTTTTAATCGGGC‑GTTTAATTTTTTTC‑TTCCAA‑C‑CGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGaaaaa < 1:604140/239‑1 (MQ=255) tAAAAGAAAATTTTGTTCG‑TTA‑TCATCC‑GTTTTTTTTTATTGTTCAGACGGGCGTTTGATTTGTTGTC‑TC‑CAC‑C‑CGCCATGAGAAACTCACGCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGTCAGTCCATTGAAAAACGGGGTaaaga < 2:401509/239‑1 (MQ=255) aaTTAGTTCGCTAA‑TCAGCC‑GTTATA‑TTTATTGTTTAAACTGGCGGTTGAATTGTTGTC‑TT‑CAA‑C‑CGCCATTAGAAACTCACTCTGCCCACAGCGCCAGTGCCAAAGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCCGGCCATTTAAAAAAGGGGTAAAGAAAGGca > 1:858271/1‑236 (MQ=255) gTTCGCTAA‑TCAGCC‑GTTATA‑TTTATTGTTTAAACTGGCGGTTGAATTTTTGTT‑TT‑CCA‑C‑CCCCATGAGAAACTCACTCTTCCCCCAACCCCCGTGCCCACGTTTTTTCCTCATCCGTACGCTCGTTTTCCCCCCGGCCGGACAAAAAATTCCTTTACAATTTAGGTAATTAAACGAGGTGTTCCTTCGTTGCTTCCGCCCATCCCTTTAAAAAAAGGGGAAAGAAAAGCACATgaggg > 2:1073900/1‑238 (MQ=255) ttCGCTAA‑TCAGCC‑GTTATA‑TTTATTGTTTAAACTGGCGGTTGAATTGTTGTC‑TT‑TCA‑C‑CGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCACCGTTTTATCATCATCCGTACTCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCCAGTTGTTTCTGCGTTTCTGCCGCCCGTCCCTTGGAAAAAGGGGGTAAGACAGGCACCTgagggg > 2:863741/1‑237 (MQ=255) cctaaataGCC‑TTTTTT‑TTTTTTGTTTAAACCGGGGGTTTAATTTTTTTC‑TC‑CAACC‑CGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGaa < 2:343433/232‑1 (MQ=255) gCC‑TTTATA‑TTTTTTGTTAAAACTGGGGGTTGAATTGTTGTC‑TA‑CAA‑C‑CGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGc < 1:1113816/239‑1 (MQ=255) tctttttta‑ttttttGTTAAACCTGGTGTGTGATTTTTTTTT‑TA‑CAC‑C‑CGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGc < 1:140672/232‑1 (MQ=255) cctttATA‑TTTTTTTTTTAAAGTGGCGGTTAATTTTTTGTC‑TC‑AAA‑C‑CGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGCa < 1:944003/236‑1 (MQ=255) tttttttGTTTAACGTGGGGTTTGATTTGTTTTC‑TC‑AAC‑C‑CGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGCATATTTaa < 1:863741/238‑1 (MQ=255) tattGTTTAAACTGGCGGTTGAATTGTTGTC‑TT‑CAA‑C‑CGCCATGAGAAAATCAATCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCCTACGCCCGTTTACCGCCGGGCTGGGCAAAAAATGCTTTAACAATTTAGGTAATAAATCGAGGTTTTCCTGCGTTGCTGCCGCCCGTCCATTGAAAAACGGGGTAAAGAAAAGCACATGAGGGGAATTATCCAGCATATTTaaag > 2:514476/1‑236 (MQ=255) attGTTTAAACTGGCGGTTGAATTGTTGTC‑TT‑TAA‑C‑CGCCATGAGAAACTCACTCTGCCCCCAGCGCCAGTGCCAACGTTTTATCATCATTCGTAAGCTCGTTTACCCCCGGGCTGGACAAAAACTGCTTTAACAATTTAGGAAATAAATAGAGTTGTTCCCGCGTTGCTGCCGCCAGTTCATTCAAAAAAGGGGTAAAGAAAGGCACATGAGGAGGACTATTCCGCATCTTTAACGc > 1:60681/1‑238 (MQ=255) tAAACTGGCGGTTGAAATGTTGTC‑TT‑CAA‑C‑CGCCATGAGAAAATCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTTAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGCATATTTAAAGCCAGTc > 1:442125/1‑237 (MQ=255) cggcgggTTAAATTTTTCTC‑TC‑CAC‑C‑CGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCAGCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGCATATTTAACGCCAGTCGCTGGa < 1:1158122/234‑1 (MQ=255) ggggtgTTTAATTTTTTTCTTT‑CAA‑C‑CGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGCATATTTAACGCCAGTCGCTGGa < 1:302663/234‑1 (MQ=255) cGGGTGAATTTTTGTC‑TT‑CAA‑A‑CGCCATGAGAAACTCACTCTTCCCACAGCGCCAGTGCCCACGTTTTATCATCATCCCTACGCTCGCTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTTCTGCGTTGCTGCCGCCCGTCCCTTGAAAAACGGGGTGAAACAAAGCACATAGGGGGAATTCTCCAGCATATTTAACGCCAATTGCTgggaacc > 2:470340/1‑234 (MQ=255) ttgtC‑TA‑CAC‑C‑CGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGCATATTTAACGCCAGTCGCTGGATACCATCCGTAAAtg < 1:514476/239‑1 (MQ=255) gtC‑TT‑CAA‑C‑CGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGCATATTTAACGCCAGTCGCTGGATCCCATCCGTAAAt > 2:406874/1‑236 (MQ=255) | TGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTATACC‑ATTGATCCTTTATTGGTCA‑CCTATCCAAAAGAAGAAGCCGACGAGC‑TAAGAGCAAAATTAGTTCGCTAA‑TCAGCC‑GTTATA‑TTTATTGTTTAAACTGGCGGTTGAATTGTTGTC‑TT‑CAA‑C‑CGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGCATATTTAACGCCAGTCGCTGGATACCATCCGTAAATG > NC_000913/2151496‑2151925 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |