Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,151,689 G→T 38.6% intergenic (+43/+22) yegJ → / ← yegK uncharacterized protein/ser/thr phosphatase‑related protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,151,6890GT38.6% 13.4 / 10.0 34intergenic (+43/+22)yegJ/yegKuncharacterized protein/ser/thr phosphatase‑related protein
Reads supporting (aligned to +/- strand):  ref base G (14/6);  new base T (6/7);  total (20/14)
Fisher's exact test for biased strand distribution p-value = 2.76e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.64e-01

TGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTATACCATTGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTGAATTGTTGTCTTCAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGCATATTTAACGCCAGTCGCTGGATACCATCCGTAAATG  >  NC_000913/2151496‑2151925
                                                                                                                                                                                                       |                                                                                                                                                                                                                                                
tGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTTTAACATTTATTCCTTTTTTGTCACCCAATCCAAAAAAGAAAGCGACGAGCTAAAAGGAAAAATAATTTGCTTATCAACCGGTTTATTTTTTTTTTTAAATTGGGGGTTTGATTTTTTTTTTTAACACCCCGTAAAAAACACACTTTCCCcacacccagcg                                                                                                                                                                                                    >  1:477713/1‑231 (MQ=255)
              aaaaCAAAAAAGAAGATATTTCAGCCTGGATGTATACGCGGGGGGATAAAATTTACGGCGTTTATCCCATTGATCCTTTATTGGTCCCCTACCCAAAAGAAGAAGCCGACGAGCTAAGAGAAAAATTAGTTCGCTAATCACCCGTTATATTTATTGTTTAAACTGGCGTTTGATTTGTTGTCTTCAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTatcat                                                                                                                                                                                   <  1:436470/239‑1 (MQ=255)
                    aaaaaaaaGATATTTCAGCTGGGATTTATGCGGGGGGGAAAAAAATTACCGGGGTTTATACCATTACTCTTTTTTGGTTCCCCTAACAAAAAGAAAAAGCCGAGGGGAAAAGAAAAAAATTTGTTCGCTATCCGCCTTTTATATTTTTTGTTAAAACGGGGGTTTAATTTGTTGTTTCAAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCg                                                                                                                                                                             <  2:1077744/237‑1 (MQ=255)
                                   cagatggtttGTATCCGCGGGGGGATAAAATTTGCGGCGTTTATACCATTTATCTTTTATTGTTCCCCTATCAAAAAGAAGAAGCCGACGAGATAAGAGAAAATTTAGTCCGTAACTCAGCCTTTATTTTTTTTTTTTAACCGGGCGTTTGATTTTTTTTCTACAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTAccg                                                                                                                                                              <  2:477713/230‑1 (MQ=255)
                                                  cgcgggGGAAAAAAATTAAGGGGGTTTATACAATTGACCTTTTATGGTCCCCCTACCAAAAAAAAGAAGCCGAGGAGCTAAGAGAAAATTTATTCCCTAATTCAGCCTTTATTTTTTTTTTTAAAACGGGGGGTTAATTTTTTGTCTCCAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACaaaa                                                                                                                                               <  1:653900/239‑1 (MQ=255)
                                                                  ttACGGGGTTTTACCAATTGTCCTTTTATTGGCCCCCTACCAAAAAAAAGAGCCCAAGAACATAAAACAAAATTTAGTCCGTAAATCAGCTTTTTTATTTTTTTTTAAACCGGGGGGTTAATTTTTTTTCTCCAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTc                                                                                                                               <  1:614736/239‑1 (MQ=255)
                                                                                     tGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTGAATTGTTGTCTTCAACCGCCCTGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTTTTATCATCCGTACGCCCGGTTACCCCCGGGCTGGACAAAAACTGCTTTAACAATTCAg                                                                                                                             >  1:463056/1‑223 (MQ=255)
                                                                                     tGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTGAATTGTTGTCTTCAACCGCCATGAGGAACTCAATCTGCCCACAGAGCCAGTGCCCACGGTTTATCATCATCCGTACGGCCGTTTCACGCCGGGCTGGACAAAAACTGCTTTAACAAATCAGGTAATAAAACGAggtt                                                                                                             >  1:793499/1‑236 (MQ=255)
                                                                                     tGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTGAATTGTTGTCTTCAACCGCCATGAGAAACTCAATCTTCCCACAGCGCCCGTGCCAACGGTTTTTCATCCTCCGTCCGCTCGTTTACCGCCGGGCTGGACAAAAACTGCCTTAAAAATTCAGGGAAAAAAACGAgttg                                                                                                             >  1:895467/1‑239 (MQ=255)
                                                                                      gATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTGAATTGTTTTCTTCAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGGGCCAACGGTTTATCATCATCCGTACGCTCGCTTACCCCCGGGCTGGACAAAAAACGCTTTAACAATTCAGGGAATAAATCGAgttgt                                                                                                            >  2:1070438/1‑239 (MQ=255)
                                                                                         ccTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTGAAATGTTGTTTTCAACCGCCAAGAGAAACTCAATCTGCCCACAGCGCCAGTGCCAACGGTTTTTCATCATCCGTACGCTCGTTTAACGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAgttgtt                                                                                                           >  1:1048379/1‑237 (MQ=255)
                                                                                            ttATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTGAATTTTTTTCTTCCACCGCCATTAGAAAATCACTCTGCCCACAGCGCCAGTGCCAACCGTTTTTCCTTCTCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAAACGCTTTAACAATTTAGGGAAAAAATAGAattttttccgc                                                                                                      >  2:604140/1‑234 (MQ=255)
                                                                                            ttATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTGAATTGTTGTCTTCAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGGTGTTCCTGc                                                                                                      >  1:801298/1‑239 (MQ=255)
                                                                                                     aCCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTGAATTGTTTTTTTCAACCGCCATGAGAAACTCACTCTTCCCACAGCGCCAGTGCCACAGGTTTTTCATCATCCGCACGCTCGTTTACCGCCGGGCTGGGCCAAAACCGGTTTTACCATTCAGGTAATTAATCGAGTTGGTCCTGCGTttctgc                                                                                              >  2:1158122/1‑238 (MQ=255)
                                                                                                     aCCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTGAATTGTTGTCTTCAACCGCCATGAGAAAATCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTtgctgc                                                                                              >  1:492121/1‑238 (MQ=255)
                                                                                                          aTCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTGTATTTTTTCCTTCAACCGCCATTAGAAACTCACTCTGCCCCCAGCGCCACGTCCAACCGTTTTTTATCAACCGTACGCCCGCTTTCCGCCCGGCTGGACCAAAAACCCTTTTACAAATCAGGTAATAAATCGAGTTGTTCCTCCGTTGCTgtcgccc                                                                                         >  2:502177/1‑238 (MQ=255)
                                                                                                                     gaagCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTTAATTGTTGACTTTAACCGCCATGAGGAAATTACTCTGCCCACAGGGTCAGTGCCAACGTTTTATCATCATCCGTTCGCTCGTTTACCGCCGGGGTGGACAAAAAATGCTTTTACAATTCATGGACTAAATCGAGTTTTTCCTGCGGTGCTTGCTCCCGTCCATTGata                                                                              >  2:252931/1‑237 (MQ=255)
                                                                                                                      aagCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTGAATTTTTTTCTTTAAACGCCATGAGAAACTCACTCTGCCCACAACGCCAGTGCCAACGTTTTATTATCATCCGCAAGCCCGTTTACCCCCGGGCTGGACACAAAATGCATTATAAATTTAGGGAATAAAACGAGTTGTTTCTGCGTTGCTGCCGCCAGTCCAtttcaaa                                                                             >  2:808528/1‑234 (MQ=255)
                                                                                                                       agCCGACGAGTAAAAAAAAAATTTAGTCCTTTAACCACCCTTTATTTTTTTTTTTTAATCGGGCGTTTAATTTTTTTCTTCCAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGaaaaa                                                                            <  1:604140/239‑1 (MQ=255)
                                                                                                                                   tAAAAGAAAATTTTGTTCGTTATCATCCGTTTTTTTTTATTGTTCAGACGGGCGTTTGATTTGTTGTCTCCACCCGCCATGAGAAACTCACGCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGTCAGTCCATTGAAAAACGGGGTaaaga                                                                 <  2:401509/239‑1 (MQ=255)
                                                                                                                                            aaTTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTGAATTGTTGTCTTCAACCGCCATTAGAAACTCACTCTGCCCACAGCGCCAGTGCCAAAGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCCGGCCATTTAAAAAAGGGGTAAAGAAAGGca                                                           >  1:858271/1‑236 (MQ=255)
                                                                                                                                                 gTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTGAATTTTTGTTTTCCACCCCCATGAGAAACTCACTCTTCCCCCAACCCCCGTGCCCACGTTTTTTCCTCATCCGTACGCTCGTTTTCCCCCCGGCCGGACAAAAAATTCCTTTACAATTTAGGTAATTAAACGAGGTGTTCCTTCGTTGCTTCCGCCCATCCCTTTAAAAAAAGGGGAAAGAAAAGCACATgaggg                                                   >  2:1073900/1‑238 (MQ=255)
                                                                                                                                                  ttCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTGAATTGTTGTCTTTCACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCACCGTTTTATCATCATCCGTACTCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCCAGTTGTTTCTGCGTTTCTGCCGCCCGTCCCTTGGAAAAAGGGGGTAAGACAGGCACCTgagggg                                                  >  2:863741/1‑237 (MQ=255)
                                                                                                                                                      cctaaataGCCTTTTTTTTTTTTGTTTAAACCGGGGGTTTAATTTTTTTCTCCAACCCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGaa                                                <  2:343433/232‑1 (MQ=255)
                                                                                                                                                              gCCTTTATATTTTTTGTTAAAACTGGGGGTTGAATTGTTGTCTACAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGc                                       <  1:1113816/239‑1 (MQ=255)
                                                                                                                                                               tcttttttattttttGTTAAACCTGGTGTGTGATTTTTTTTTTACACCCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGc                                       <  1:140672/232‑1 (MQ=255)
                                                                                                                                                                cctttATATTTTTTTTTTAAAGTGGCGGTTAATTTTTTGTCTCAAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGCa                                      <  1:944003/236‑1 (MQ=255)
                                                                                                                                                                        tttttttGTTTAACGTGGGGTTTGATTTGTTTTCTCAACCCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGCATATTTaa                               <  1:863741/238‑1 (MQ=255)
                                                                                                                                                                           tattGTTTAAACTGGCGGTTGAATTGTTGTCTTCAACCGCCATGAGAAAATCAATCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCCTACGCCCGTTTACCGCCGGGCTGGGCAAAAAATGCTTTAACAATTTAGGTAATAAATCGAGGTTTTCCTGCGTTGCTGCCGCCCGTCCATTGAAAAACGGGGTAAAGAAAAGCACATGAGGGGAATTATCCAGCATATTTaaag                             >  2:514476/1‑236 (MQ=255)
                                                                                                                                                                            attGTTTAAACTGGCGGTTGAATTGTTGTCTTTAACCGCCATGAGAAACTCACTCTGCCCCCAGCGCCAGTGCCAACGTTTTATCATCATTCGTAAGCTCGTTTACCCCCGGGCTGGACAAAAACTGCTTTAACAATTTAGGAAATAAATAGAGTTGTTCCCGCGTTGCTGCCGCCAGTTCATTCAAAAAAGGGGTAAAGAAAGGCACATGAGGAGGACTATTCCGCATCTTTAACGc                            >  1:60681/1‑238 (MQ=255)
                                                                                                                                                                                  tAAACTGGCGGTTGAAATGTTGTCTTCAACCGCCATGAGAAAATCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTTAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGCATATTTAAAGCCAGTc                       >  1:442125/1‑237 (MQ=255)
                                                                                                                                                                                      cggcgggTTAAATTTTTCTCTCCACCCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCAGCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGCATATTTAACGCCAGTCGCTGGa                 <  1:1158122/234‑1 (MQ=255)
                                                                                                                                                                                       ggggtgTTTAATTTTTTTCTTTCAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGCATATTTAACGCCAGTCGCTGGa                 <  1:302663/234‑1 (MQ=255)
                                                                                                                                                                                          cGGGTGAATTTTTGTCTTCAAACGCCATGAGAAACTCACTCTTCCCACAGCGCCAGTGCCCACGTTTTATCATCATCCCTACGCTCGCTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTTCTGCGTTGCTGCCGCCCGTCCCTTGAAAAACGGGGTGAAACAAAGCACATAGGGGGAATTCTCCAGCATATTTAACGCCAATTGCTgggaacc             >  2:470340/1‑234 (MQ=255)
                                                                                                                                                                                                     ttgtCTACACCCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGCATATTTAACGCCAGTCGCTGGATACCATCCGTAAAtg  <  1:514476/239‑1 (MQ=255)
                                                                                                                                                                                                       gtCTTCAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGCATATTTAACGCCAGTCGCTGGATCCCATCCGTAAAt   >  2:406874/1‑236 (MQ=255)
                                                                                                                                                                                                       |                                                                                                                                                                                                                                                
TGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTATACCATTGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTGAATTGTTGTCTTCAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGCATATTTAACGCCAGTCGCTGGATACCATCCGTAAATG  >  NC_000913/2151496‑2151925

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: