Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,862,658 A→G 18.6% pseudogene (946/1617 nt) glvC ← pseudogene, arbutin specific enzyme IIC component of PTS;enzyme; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system, arbutin‑like IIB component; PTS system, arbutin‑like IIC component

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,862,6580AG18.6% 112.4 / 6.0 65pseudogene (946/1617 nt)glvCpseudogene, arbutin specific enzyme IIC component of PTS;enzyme; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system, arbutin‑like IIB component; PTS system, arbutin‑like IIC component
Reads supporting (aligned to +/- strand):  ref base A (36/14);  new base G (2/11);  total (39/26)
Fisher's exact test for biased strand distribution p-value = 3.19e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AACCCAATGGCGATTTGGGTCAGCATCATATCCGCGTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACCTAGTCAATCAGACCTCCGCCCATGTTGCCCACCACACCAAAGAGATACATTACGGTCGACATTGAGGCCGCCAGCACGGCGTGTACCGCAAACAGCAACGGTGAAATGAACAGGAAGGTAAATTCCAGCGGTTCGGTAATTCCCACCAGCATGGCGGTTAAGGTTGCGGGAATCAGCAAGCCCGCCACTTTTACCCGATTTTCCGGTGCGGCAGTGAAGTACATCGCTAAAGAAATGCCCACGGCACCAAAGATTTTTGAGTTACCGTGCAGGGCAAAACCGCCTTCCGGGAACAACGATTTCAGCGGCTCGGCGCTCAAACTGAACTCTTGCAGATGCTGCGCCCAGTACATCTGAATGCCGCCTTCAACAGCTGCCGGACCAAAGATAAACTGTC  >  NC_000913/3862423‑3862894
                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                           
aaCCCAATGGCGATTTGGGTCAGCATCATATCCGCGTGGATGCTGAACATCGGGATCCAGTTTTGCGGTAAAACCTAGTCAATCAGACCTCCGCCCATGTTGCCCACCACACCAAAGAGATACATTACGGTCGACATTGAGGCCGCCAGCACGGCGTGTACCGCAAACAGCAACGGTGAAATGAACAGGAAGGTAAATTCCAGCGGTTCGGTAATTCCCACCAGCATGGCGGTTAAGGt                                                                                                                                                                                                                                                         >  1:484273/1‑239 (MQ=255)
       tGGCGATTTGGGTCAGCATCATATCCGCGTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACCTAGTCAATCAGACCTCCGCCCATGTTGCCCACCACACCAAAGAGATACATTACGGTCGACATTGAGGCCGCCAGCACGGCGTGTACCGCAAACAGCAACGGTGAAATGAACAGGAAGGTAAATTCCAGCGGTTCGGTAATTCCCACCAGCATGGCGGGTTAAGATGCgggg                                                                                                                                                                                                                                                 >  2:853302/1‑238 (MQ=255)
       tGGCGATTTGGGTCAGCATCATATCCGCGTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACCTAGTCAATCAGACCTCCGCCCATGTTGCCCACCACACCAAAGAGATACATTACGGTCGACATTGAGGCCGCCAGCACGGCGTGTACCGCAAACAGCAACGGTGAAATGAACAGGAAGGTAAATTCCAGCGGTTCGGTAATTCCCACCAGCATGGCGGGTAAGGGTGCGGGa                                                                                                                                                                                                                                                 >  1:1099932/1‑239 (MQ=255)
           gATTTGGGGTAGCATCATATCCGCGTGGTTGCTGAACAACGGGATCCAGTTTTGCGGTAAAACCTAGTCAATCAGACCTCCGCCCATGTTGCGCACCACACCAAAGAGATACATTACGGTCGACATTGAGGCCGCCAGCACGGCGTGTACCGCAAACAGCAACGGTGAAATGAACAGGAAGGTAAATTCCAGCGGTTCGGTAATTCCCACCAGCATGGCGGTTAAGGTTGCGGGAATCa                                                                                                                                                                                                                                            <  2:480124/239‑1 (MQ=255)
            aTTTGGGTCAGCATCATATCCGCGTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACCTAGTCAATCAGACCTCCGCCCATGTTGCCCACCACACCAACGAGATACATTACGGTCGACATTGCGACCGCCAGCACGGCGTGTACCGCAAACAGCAACGGTGAAATGAACAGGAAGGAAAATTCCAGCGCTCCGGTAACTCCCACCAGCATGGCGGTTAAGTGTTGCgggcaccg                                                                                                                                                                                                                                             >  2:831559/1‑234 (MQ=255)
            aTTTGGGTCAGCATCATATCCGCGTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACCTAGTCAATCAGACCTCCGCCCATGTTGCCCACCACACCAAAGAGATACATTACGGTCGACATTGAGGCCGCCAGCACGGCGTGTACCGCAAACAGCAACGGTGAAATGAACAGGAAGGTAAATTCCAGCGGTTCGGTAATTCCCACCAGCATGGCGGTTAAGGGTGCGGGGATCa                                                                                                                                                                                                                                            >  1:10248/1‑238 (MQ=255)
            aTTTGGGTCAGCATCATATCCGCGTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACCTAGTCAATCAGACCTCCGCCCATGTTGCCCACCACACCAAAGAGATACATTACGGTCGACATTGAGGCCGCCAGCACGGCGTGTACCGCAAACAGCAACGGTGAAATGAACAGGAAGGTAAATTCCAGCGGTTCGGTAATTCCCACCAGCATGGCGGTTAAGGGTGCGGGGATCa                                                                                                                                                                                                                                            >  1:584744/1‑238 (MQ=255)
            aTTTGGGTCAGCATCATATCCGCGTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACCTAGTCAATCAGACCTCCGCCCATGTTGCCCACCACACCAAAGAGATACATTACGGTCGACATTGAGGCCGCCAGCACGGCGTGTACCGCAAACAGCAACGGTGAAATGAACAGGAAGGTAAATTCCAGCGGTTCGGTAATTCCCACCAGCATGGCGGGTAAGGGTTCGGGCATCa                                                                                                                                                                                                                                            >  2:1099592/1‑238 (MQ=255)
             tttGGGTCAGCATCATATCCGCGTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACCTAGTCAATCAGACCTCCGCCCATGTTGCCCACCACACCAAAGAGATACATTACGGTCGACATTGAGGCCGCCAGCACGGCGTGTACCGCAAACAGCAACGGTGAAATGAACAGGAAGGTAAATTCCAGCGGTTCGGTAATTCCCACCAGCATGGCGGTTCAAGGTGCGGGAAACAgg                                                                                                                                                                                                                                          >  1:671438/1‑238 (MQ=255)
             tttGGGTCAGCATCATATCCGCGTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACCTAGTCAATCAGACCTCCGCCCATGTTGCCCACCACACCAAAGAGATACATTACGGTCGACATTGAGGCCGCCAGCACGGCGTGTACCGCAAACAGCAACGGTGAAATGAACAGGAAGGTAAATTCCAGCGGTTCGGTAATTCCCACCAGCATGGCGGTTAAGGTTGCGGGAATCAGc                                                                                                                                                                                                                                          <  1:893081/239‑1 (MQ=255)
             tttGGGTCAGCATCATATCCGCGTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACCTAGTCAATCAGACCTCCGCCCATGTTGCCCACCACACCAAAGAGATACATTACGGTCGACATTGAGGCCGCCAGCACGGCGTGTACCGCAAACAGCAACGGTGAAATGAACAGGAAGGTAAATTCCAGCGGTTCGGTAATTCCCACCAGCATGGCGGGTAAGGGTGCggggaacac                                                                                                                                                                                                                                            >  1:1168003/1‑232 (MQ=255)
                     aGCATCATATCCGCGTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACCTAGTCAATCAGACCTCCGCCCATGTTGCCCACCACACCAAAGAGATACATTACGGTCGACATTGAGGCCGCCAGCACGGCGAGTACCGCAAACAGCAACGGGGAAATGAAAAGGAAGGCAAATTCCAGAGGTTCGGTAATTCCCACCAGCATGGCGGTTAAAGGTGGTGGGATTAGCAAGtccc                                                                                                                                                                                                                                 >  2:29728/1‑237 (MQ=255)
                            tatCCGCGTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACCTAGTCAATCAGACCTCCGCCCATGTTGCCCACCACACCAAAGAGATACATTACGGTCGACATTGAGGCCGCCAGCACGGCGTGTACCGCAAACAGCAACGGTGAAATGAACAGGAAGGTAAATTCCAGCGGGTCGGGAATTCCCACCAGCATGGCGGTGTGGGTTGCGGGCATCAACCAGccccgacccttt                                                                                                                                                                                                                         >  2:929592/1‑230 (MQ=255)
                                  cgTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACCTAGTCAATCAGACCTCCGCCCATGTTGCCCACCACACCAAAGAGATACATTACGGTCGACATTGAGGCCGCCAGCACGGCGTGTACCGCAAACAGCAACGGTGAAATGAACAGGAAGGTAAATTCCAGCGGTTCGGTAATTCCCACCAGCATGGCGGGTAAGGTTTCGGGAATCAGAAAGCCCGCCCCCTTTAccc                                                                                                                                                                                                                  >  1:143025/1‑237 (MQ=255)
                                  cgTGGTTGCTGAACATCGGGATACAGTTTTGCGGCAAAACCTAGTCAATCAGACCTCCGCCCATGTTGCCCACCACACCAAAGAGATACATTACGGTCGACATTGAGGCCGCCAGCACGGCGAGTACCGCAAACAGCAACGGTGAAATGAAACGGAAGGTAAATTCCAGCGGTGCGGTAATTCCAACAAGCATGGCGGTTGAAGTTGTGGGAATAAGCAAGCCACGTCACTTTCAccgg                                                                                                                                                                                                                 >  2:326370/1‑237 (MQ=255)
                                      gTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACCTAGTCAATCAGACCTCCGCCCATGTTGCCCACCACACCAAAGAGATACATTACGGTCGACATTGAGGCCGCCAGCACGGCGTGTACCGCAAACAGCAACGGTGAAATGAACAGGAAGGTAAATTCCAGCGGTTCGGTAATTCCCACCAGCATGGCGGTTAAGGGTGCGGGGGTCAGCAAACCCCCCCCCTTTccccccgttt                                                                                                                                                                                                            >  2:672052/1‑233 (MQ=255)
                                         gCTGAACATCGGGATCCAGTTTTGCGGTAAAACCTAGTCAATCAGACCTCCGCCCATGTTGCCCACCACACCAAAGAGATACATTACGGTCGACATTGAGGCCGCCAGCACGGCGTGTACCGCAAACAGCAACGGTGAAATGAACAGGAAGGTAAATTCCAGCGGTTCGGTAATCCCCACCAGCATGGCGGTCACTAGTGAGGGAGCCCGTCACCCCCCCCCTCTTACCCGATTTTct                                                                                                                                                                                                          >  2:779348/1‑237 (MQ=255)
                                            gAACAGCGGGACCCAGTTTTGCGGTAAAACCTAGTCAATCAGCCCCCCGCCCATGTTGCCCACCACACCAAAGAGATACATTAGGGTCGACATTGAGGCCGCCAGCACGGCGTGTACCGAAAACAGCAACGGTGAAATGAACAGGAAGGAAAATCCCAGCGGTTCGGTAATCCCCACCAGCATGGGGGTTAAGGTTGCGGGAATCAGAAAGCCCGCCACTTTTACCCGATTTTCCGGTg                                                                                                                                                                                                      <  1:1024951/239‑1 (MQ=255)
                                                         cAGTTTTGCGGTAAAACCTAGTCAATCAGACCTCCGCCCATGTTGCCCACCACACCAAAGAGATACATTACGGTCGACATTGAGGCCGCCAGCACGGCGTGTACCGCAAACAGCAACGGTGAAATGAACAGGAAGGTAAATTCCAGCGGTTCGGTAATTCCCACCAGCATGGCGGTTAAGGGTGCGGGGATCAGCAAGACCCCCCCTTTTTCCCGATTTTTCCGTGGGGCCGGGAAGTa                                                                                                                                                                                         >  1:414044/1‑239 (MQ=255)
                                                              ttGCGGTAAAACCTAGTCAATCAGACCTCCGCCCATGTTGCCCACCACACCAAAGAGATACATTACGGTCGACATTGAGGCCGCCAGCACGGCGTGTACCGCAAACAGCAACGGTGAAATGAACAGGAAGGTAAATTCCAGCGGTTCGGTAATTCCCACCAGCATGGCGGGTTAGGGTGGGGGGATCAGCAAGCCCCCCACTTTTAACCGATTTTCCGGTGGGGCGGGGAAGGACacc                                                                                                                                                                                     >  1:1183473/1‑236 (MQ=255)
                                                                 cGGTAAAACCTAGTCAATCAGACCTCCGCCCATGTTGCCCACCACACCAAAGAGATACATTACGGTCGACATTGAGGCCGCCAGCACGGCGTGTACCGCAAACAGCAACGGTGAAATGAACAGGAAGGTAAATTCCAGCGGTTCGGTAATTCCCACCAGCATGGCGGTTAAGGGTGCGGGGAACAGCAAACCCCCCCATTTTACCCGATTTTTCGGGGCGGGAGGGAAGGACATCGCtc                                                                                                                                                                                >  1:725604/1‑238 (MQ=255)
                                                                      aaaCCTAGTCAATCAGACCTCCGCCCATGTTGCCCACCACACCAAAGAGATACATTACGGTCGACATTGAGGCCGCCAGCACGGCGTGTACCGCAAACAGCAACGGTGAAATGAACAGGAAGGTAAATTCCAGCGGTTCGGTAATTCCCACCAGCATGGCGGTTAAGGGTGCGGGAATCAGCCAGCCCGCCACATTTACCCGGTTTTTCGGGGCGGCAGTGAAAGAAAACGCCAAAGaa                                                                                                                                                                          >  1:1179920/1‑239 (MQ=255)
                                                                                  tCAGACCTCCGCCCATGTTGCCCACCACACCAAAGAGATACATTACGGTCGACATTGAGGCCGCCAGCACGGCGTGTACCGCAAACAGCAACGGTGAAATGAACAGGAAGGTAAATTCCAGCGGTTCGGTAATTCCCACCAGCATGGCGGTTAAGGGTGGGGGAATCAACAAACCCCCCCCCTTTTACCCATTTTTCGGGGCGGCAGGGAAGGACAACCCTCAAAAAAAACCCACCGc                                                                                                                                                             >  1:804903/1‑238 (MQ=255)
                                                                                  tCAGACCTCCGCCCATGTTGCCCACCACACCAAAGAGATACATTACGGTCGACATTGAGGCCGCCAGCACGGCGTGTACCGCAAACAGCAACGGTGAAATGAACAGGAAGGTAAATTCCAGCGGTTCGGTAATTCCCACCAGCATGGCGGGTAAAGTTGCGGGGATCAGCAAAACCGCCCCTTTTACCCGATTTTTCGGTGGGGCAGGGAAGGAAATCGCTAAAAAAAAGCCCACCGc                                                                                                                                                             >  1:1100845/1‑238 (MQ=255)
                                                                                   cAGACCTCCGCCCATGTTGCCCACCACACCAAAGAGATACATTACGGTCGACATTGAGGCCGCCAGCACGGCGTGTACCGCAAACAGCAACGGTGAAATGAACAGGAAGGTAAATTCCAGCGGTTCGGTAATTCCCACCAGCATGGCGGATTAAGGTGCGGGGATCAGCAAGGCCGCCACCTTTATCCGATTTTTCGGTGCGGCCGGGGAGGACAATCCTAAAGAAATGCCCACGGCa                                                                                                                                                            >  1:730278/1‑238 (MQ=255)
                                                                                           cGCCCATGTTGCCCACCACACCAAAGAGATACATTACGGTCGACATTGAGGCCGCCAGCACGGCGTGTACCGCAAACAGCAACGGTGAAATGAACAGGAAGGTAAATTCCAGCGGTTCGGTAATTCCCACCAGCATGGCGGTTAAGGGTGGGGGGAACAACAAAACCCCCCCTTTTAACCCCTTTTTCCGGGCGGCAGGGAAGGACATCCCCAAAAAAAACCCCCCGCGCACCAAAgtt                                                                                                                                                    >  2:578228/1‑237 (MQ=255)
                                                                                                    tGCCCACCACACCAAAGAGATACATTACGGTCGACATTGAGGCCGCCAGCACGGCGTGTACCGCAAACAGCAACGGTGAAATGAACAGGAAGGTAAATTCCAGCGGTTCGGTAATTCCCACCAGCATGGCGGTTTAGGGTTGGGGGAACAGCAAGGCCCCCCCCTTTTCCCGGTTTTCCCGTGCGGGCGTGAAATACCTCCCTAAAGAAAAGCCCCCAGCACCCAAGATTTTTTAGtt                                                                                                                                           >  1:992772/1‑238 (MQ=255)
                                                                                                    tGCCCACAACACAAAAGAGATACATTACGGTCGACATTGAGGCCGCCAGCACGGCGTGTACCGCAAACAGCAACGGTAAAATGAACAGGAAGGAAAATTCCAGCGGTTCGGTAATCCCCACCAGCAGGGCGGTTAAGGTTGCGGAAATCAGCAAGCCCGCCACTTTTACCCGATTTTCCGGTGCGGCAGTGAAGTACATCGCTAAAGAAATGCCCACGGCACCAAAGATTTTTGAGTTa                                                                                                                                         <  1:672052/239‑1 (MQ=255)
                                                                                                     gCCCACCACACCAAAGAGATACATTACGGTCGACATTGAGGCCGCCAGCACGGCGTGTACCGCAAACAGCAACGGTGAAATGAACAGGAAGGTAAATTCCAGCGGTTCGGTAATTCCCACCAGCATGGCGGTTTAAGGTTCGGGAATCAGCAAACCCCCCCCTTTTACCCCATTTTTCCGGGCGGCCGTGAAATAAAACGCGAAAGAAAAACCCCCCGCCCCAAAGATTTTTGAGttt                                                                                                                                          >  1:1133954/1‑237 (MQ=255)
                                                                                                     gCCCACCACACCAAAGAGATACATTACGGTCGACATTGAGGCCGCCAGCACGGCGTGTACCGCAAACAGCAACGGTGAAATGAACAGGAAGGTAAATTCCAGCGGTTCGGTAATTCCCACCAGCATGGCGGTTAAGGGTGCGGGGAATAGCCAACCCCCCCCCTTTTCCCCGTTTTTCGGTGGGGCGGGGAAGGACATCGCTAAAAAAAAGCCCACCGCCCCCAAAATTTTTTGGTTa                                                                                                                                         >  2:494058/1‑238 (MQ=255)
                                                                                                                     agaTACATTACGGTCGACATTGAGGCCGCCAGCACGGCGTGTACCGCAAACAGCAACGGTGAAATGAACAGGAAGGTAAATTCCAGCGGTTCGGTAATTCCCACCAGCATGGCGGTTTAGGGTGCGGGGATCAGCAAGCCCCCCCCCTTTACCCCATTTTCCGGTGCGGCAGGGTAGAAAAACGCTAAAAAAAAGCCCCCCGCACCCAAGAGTTTTTAGTTTCCGTGCCGGGGaaaaa                                                                                                                        >  2:1118046/1‑237 (MQ=255)
                                                                                                                       aTACATTACGGTCGACATTGAGGCCGCCAGCACGGCGTGTACCGCAAACAGCAACGGTGAAATGAACAGGAAGGTAAATTCCAGCGGTTCGGTAATTCCCACCAGCATGGCGGTTTAAGTTGCGGGGATCAGCAAAGCCCCCACCTTTTCCCCATTTTTCCGGGCGGCAGGTGAGTAAAACGCCCAAAAAAAACCCCACGCACCCAAAAATTTTTTGGTACCCTTGCAGGGAAAAAcc                                                                                                                       >  1:692032/1‑238 (MQ=255)
                                                                                                                        tACATTACGGTCGACATTGAGGCCGCCAGCACGGCGTGTACCGCAAACAGCAACGGTGAAATGAACAGGAAGGTAAATTCCAGCGGTTCGGTAATTCCCACCAGCATGGCGGTTAAGGGTGCGGGGATCAGCAAACCCGCCACTTTTTCCCGCTTTTCCGGTGCGGCAGTGAAGTACATCGCTAAAAAAATGCCCCCGGCACCAAAGATTTTTTAGTTACCGTGCAGGGCAAAACCGc                                                                                                                    >  1:1083222/1‑238 (MQ=255)
                                                                                                                             tACGGTCGACATTGAGGCCGCCAGCACGGCGTGTACCGCAAACAGCAACGGTGAAATGAACAGGAAGGTAAATTCCAGCGGTTCGGTAATTCCCACCAGCATGGCGGGTTAAGGTGTGGGGACCAGCCAAACCGCCACCTTTATCCGATTTTTCCGGGCGGCAGGGAAGTAAAACCCGCCAGAAAAACCCACGGCCCCCAAGGATTTTTGGGTTTCGGGCGGGGCAAAAcaccctctc                                                                                                                >  2:1164301/1‑230 (MQ=255)
                                                                                                                               cGGTCGACATTGAGGCCGCCAGCACGGCGTGTACCGCAAACAGCAACGGTGAAATGAACAGGAAGGTAAATTCCAGCGGTTCGGTAATTCCCACCAGCATGGCGGGTAAGGATGCGGGAATCAGCCAAACCGCCCCTTTTACCCCGATTTTCGGGGGGGCCGTGGAGGACAACGCTCAAAAAAAACCCACGGCCACAAAGGATTTTTAGTTTACGCGCCGGGCAAAAAAGCCCTCCcgg                                                                                                            >  2:768516/1‑239 (MQ=255)
                                                                                                                                ggTAGACATGGAGGCCGCCAGCACGGCGTGTCCCGAAAACAGCAACGGTAAAATGAACGGGAAGGTAAATCCCAGCGGTTCGGTAATCCCCCCCGGCGTGGGGGTTAGGGTGGCGGAAATCAGCAAGCCCGCCACTTTTACCCGATTTTCCGGTGCGGCAGTGAAGTACATCGCTAAAGAAATGCCCACGGCACCAAAGATTTTTGAGTTACCGTGCAGGGCAAAACCGCCTTCCggg                                                                                                            <  2:1100845/238‑1 (MQ=255)
                                                                                                                                 gTCGACATTGAGGCCGCCAGCACGGCGTGTACCGCAAACAGCAACGGTGAAATGAACAGGAAGGTAAATTCCAGCGGTTCGGTAATTCCCACCAGCATGGCGGGTAAAGTTGCGGGGAACAGCCAACCCCCCACCTTTTCCCCCTTTTTCCGTGCGGCAGGGGAGAACACACCTCAAAAAAAGCCCCCGGCACCAAAACATTTTGAGTTAACGTGCAGGGCAAAAACCCCTTCCcggt                                                                                                           >  2:424778/1‑237 (MQ=255)
                                                                                                                                    gACATTGAGGCCGCCAGCACGGCGTGTACCGCAAACAGCAACGGTGAAATGAACAGGAAGGTAAATTCCAGCGGTTCGGTAATTCCCACCAGCATGGCGGGTAAGGGTTCGGGAATCAGCAAACCCCCCCCTTTTTCCCGATTTTTCGGTGCGGCAGGGAAATACATCGCTAAAGAAAAGCCCACGGCCCCAAAGATTTTTTAGTTACCGTGCAGGGGAAAAACCCCCTCCGGGaaaaa                                                                                                       >  1:65011/1‑239 (MQ=255)
                                                                                                                                                gccAGCACGGCGTGTACCGCAAACAGCAACGGTGAAATGAACAGGAAGGTAAATTCCAGCGGTTCGGTAATTCCCACCAGCATGGCGGTTTAGGGTGGGGGAAGCAACCAGCCCCCCACCTTTTACCCATTTTTCGGGGCGGCGGGGAAGTACACCGCCAAAGAAATTACCCCGGGCCCAAAGAATTTTTTTGTATCGGGCCGGGCGAAAACGACCTTCCGGGACAAAGAttttcaagg                                                                                           >  2:15665/1‑233 (MQ=255)
                                                                                                                                                gccAGCACGGCGTGTACCGCAAACAGCAACGGTGAAATGAACAGGAAGGTAAATTCCAGCGGTTCGGTAATTCCCACCAGCATGGCGGGTAAAGGTTCGGGGATCAAGAAAGCCCCCCCTTTTTACCGATTTTTCGGTGCGGCGGGGAAGTAAATAGCTTAAAAAAATCCCCCGGCCCCCCAGAATTTTTAGTTAACCGGCCCGGGAAAAACGCCCTCCCGGGaaaaaggtctctacg                                                                                             >  2:573412/1‑228 (MQ=255)
                                                                                                                                                gccAGCACGGCGTGTACCGCAAACAGCAACGGTGAAATGAACAGGAAGGTAAATTCCAGCGGTTCGGTAATTCCCACCAGCATGGCGGGTAAAGGTGCGGGGAACAAGAAGGCCCCCACCTTTTCCCGACTTTTCTGGGTGGCAGTGGAGGACAACGACTAAAAAAAACCCCCCGCCCCCAAGAAGTTTTTGTTACCGCGTCGGGGACAAACGCCCTCCCGGAACAACAACTTCAGCg                                                                                            >  2:21287/1‑238 (MQ=255)
                                                                                                                                                          cgggtcccGAAAACAGAAACGGTGAAATGAACAGGAGGGTAAATCCCAGCGGTGCGGAAATCCCCACCAGCAGGGCGGTTAGGGTGGGGGGAATAAGCAGGCCCGCCACTTTTACCCGATTTTCCGGTGCGGCAGTGAAGTACATCGCTAAAGAAATGCCCACGGCACCAAAGATTTTTGAGTTACCGTGCAGGGCAAAACCGCCTTCCGGGAACAACGATTTCAGCGGCTCGGCGCt                                                                                  <  1:1168160/232‑1 (MQ=255)
                                                                                                                                                          cGTGTACCGCAAACAGCAACGGTGAAATGAACAGGAAGGTAAATTCCAGCGGTTCGGTAATTCCCACCAGCATGGCGGGTAAGGGTGGGGGAAACACGAAACCCCCCCCTTTTTCCCCGTTTTTCCGTGCGGCAGTGAAGTAACACGCTAAAAAAAAGGCCCCCGCCCCCAAAGTTTTTTAGTTACCGGGCAGGGGAAAAACGCCCTTCCGGGACAACGATTTTCGCGCGTCGGGGCt                                                                                  >  2:640789/1‑238 (MQ=255)
                                                                                                                                                                cccaaaaCAGCAACGGTAAAAGGAACAGAAAGGAAAATTCCAGCGGTCCGTTAATCCCCACCAGCATGGCGGTTAGGGTGGCGGAAACCAGCAAGCCCGCCACTTTTACCCGATTTTCCGGTGCGGCAGTGAAGTACATCGCTAAAGAAATGCCCACGGCACCAAAGATTTTTGAGTTACCGTGCAGGGCAAAACCGCCTTCCGGGAACAACGATTTCAGCGGCTCGGCGCTCAAACTg                                                                           <  1:599170/235‑1 (MQ=255)
                                                                                                                                                                ccGCAAACACACAGGGTAAAATAAACAGAAAGGAAAAGTCGAGCGGTTCGGTAACTCCCCCCGGCCGGGCGGTAAGGGGTGGGGAAACCAGCAAGCCCGCCACTTTTACCCGATTTTCCGGTGCGGCAGTGAAGTACATCGCTAAAGAAATGCCCACGGCACCAAAGATTTTTGAGTTACCGTGCAGGGCAAAACCGCCTTCCGGGAACAACGATTTCAGCGGCTCGGCGCTCAAACTg                                                                           <  2:1168003/239‑1 (MQ=255)
                                                                                                                                                                          cAACGGTGAAATGAACAGGAAGGTAAATTCCAGCGGTTCGGTAATTCCCACCAGCATGGCGGTTAAGGGTTCGGGAATCAAAGAGCCCGGCACTTTTACCCGGTTTTCCGGTGCGGCAGGGGAGGACAACCCTCAAGAAATGCCCCCGGCCCCCAAGAATTTTGTGGTACCGTGCCGGGCCAAAACGCCCTCCCGGGACAAAGATTTTAGCGGGTCGGCGCGCACACtagaccacctg                                                                  >  2:615608/1‑228 (MQ=255)
                                                                                                                                                                           aggggTAAAATAAAGAGAAAGGAAAATTCCAGCGGTCGGTTAATCCCCACCAGCATGGCGGTTAAGGTGGGGGAAATCAGCAAGCCCGCCACTTTTACCCGATTTTCCGGTGCGGCAGTGAAGTACATCGCTAAAGAAATGCCCACGGCACCAAAGATTTTTGAGTTACCGTGCAGGGCAAAACCGCCTTCCGGGAACAACGATTTCAGCGGCTCGGCGCTCAAACTGAACTCTTGCAg                                                                <  1:853302/236‑1 (MQ=255)
                                                                                                                                                                               gTGAAATGAACAGAAAGGAAAATTCCAGCGTTTCGGTAATCCCCACCAGCAGGGCGGTTAAGGTTGCGGAAATCAGCAAGCCCGCCACTTTTACCCGATTTTCCGGTGCGGCAGTGAAGTACATCGCTAAAGAAATGCCCACGGCACCAAAGATTTTTGAGTTACCGTGCAGGGCAAAACCGCCTTCCGGGAACAACGATTTCAGCGGCTCGGCGCTCAAACTGAACTCTTGCAGAtg                                                             <  1:1118046/238‑1 (MQ=255)
                                                                                                                                                                                 aaaaTGAACAGGAAGGAAAATTCCGGCGTGCCGGAAATCCCCACCAGCATGGTGTTTAGGGTGGCGGGAACCAACAACCCCGCCACTTTTACCCGATTTTCCGGTGCGGCAGTGAAGTACATCGCTAAAGAAATGCCCACGGCACCAAAGATTTTTGAGTTACCGTGCAGGGCAAAACCGCCTTCCGGGAACAACGATTTCAGCGGCTCGGCGCTCAAACTGAACTCTTGCAGAtgct                                                           <  2:804903/237‑1 (MQ=255)
                                                                                                                                                                                   aaagAACAGGAAGGTAAATCCCGGCGGTCCGATAATCCCCACCAACAGGGCTGTTAAGGTTGGGGGAATCAGCAAGCCCGCCACTTTTACCCGATTTTCCGGTGCGGCAGTGAAGTACATCGCTAAAGAAATGCCCACGGCACCAAAGATTTTTGAGTTACCGTGCAGGGCAAAACCGCCTTCCGGGAACAACGATTTCAGCGGCTCGGCGCTCAAACTGAACTCTTGCAGATGCTgcg                                                        <  1:15665/236‑1 (MQ=255)
                                                                                                                                                                                   aaTGAAGAGAAGGGTAAATCCCGGGGGTTGGGTAATCCCCCCCGGCATGCGGTGTAAGTGGGCGGAAACCAGAAAGCCCGCCACTTTTACCCGATTTTCCGGTGCGGCAGTGAAGTACATCGCTAAAGAAATGCCCACGGCACCAAAGATTTTTGAGTTACCGTGCAGGGCAAAACCGCCTTCCGGGAACAACGATTTCAGCGGCTCGGCGCTCAAACTGAACTCTTGCAGATGCTgcg                                                        <  1:745465/239‑1 (MQ=255)
                                                                                                                                                                                   aaTGAACAGGAAGGTAAATTCCAGCGGTTCGGTAATTCCCCCCAGCAGGGCGGTTAAGGTGGCGGAAATCAGCAAGCCCGCCACTTTTACCCGATTTTCCGGTGCGGCAGTGAAGTACATCGCTAAAGAAATGCCCACGGCACCAAAGATTTTTGAGTTACCGTGCAGGGCAAAACCGCCTTCCGGGAACAACGATTTCAGCGGCTCGGCGCTCAAACTGAACTCTTGCAGATGCTgcg                                                        <  1:471051/239‑1 (MQ=255)
                                                                                                                                                                                     taaaCAGGAAGGAAAATCCCAGCGGTTCGATAACCCCCACCAGCATGGCGTTTAAGGTGGGGGGAACCAGCAAGCCCGCCACTTTTACCCGATTTTCCGGTGCGGCAGTGAAGTACATCGCTAAAGAAATGCCCACGGCACCAAAGATTTTTGAGTTACCGTGCAGGGCAAAACCGCCTTCCGGGAACAACGATTTCAGCGGCTCGGCGCTCAAACTGAACTCTTGCAGATGCTGCGcc                                                      <  1:1099592/237‑1 (MQ=255)
                                                                                                                                                                                     tGAACAGCAAGGTAAATACCAGCGGTTCGTTAATTCCCACCAGCATGGCGGTTTAGGTTACGGGAATCAACAAGCCCGCCACCTTTACCCGATTTTCCGGTGCGGCAGTGAAGTGCATCGCTAAAGAAATGCCCACGGCACCAAAGATTTTTGAGTTACCGTGCAGGGCAAAACCGCCTTCCGGGAACAACGATTTCAGCGGCTCGGCGCTCAAACTGAACTCTTGCAGATGCTGCGcc                                                      <  1:876063/239‑1 (MQ=255)
                                                                                                                                                                                     tGAACAGAAAGGAAAATCCCAGCGTGGCGTTAATCCCCCCCAGCTTGTGGTTTAGGGTGGCGGGAATCAGCAAGGCCGCCACTTTTACCCGATTTTCCGGTGCGGCAGTGAAGTACATCGCTAAAGAAATGCCCACGGCACCAAAGATTTTTGAGTTACCGTGCAGGGCAAAACCGCCTTCCGGGAACAACGATTTCAGCGGCTCGGCGCTCAAACTGAACTCTTGCAGATGCTGCGcc                                                      <  1:929592/239‑1 (MQ=255)
                                                                                                                                                                                     agAACAGTAAGTTAAACCCCAGTGGTGTGATATCCCCCCCCAGCATGGCGTTTAGGGGTGCGGAAACCAGCAAGCCCGCCACTTTTACCCGATTTTCCGGTGCGGCAGTGAAGTACATCGCTAAAGAAATGCCCACGGCACCAAAGATTTTTGAGTTACCGTGCAGGGCAAAACCGCCTTCCGGGAACAACGATTTCAGCGGCTCGGCGCTCAAACTGAACTCTTGCAGATGCTGCGcc                                                      <  2:725604/238‑1 (MQ=255)
                                                                                                                                                                                          aGGAAGTTAAATTCCGGCGGTTTGGTAATCCCCACCAGGATGGCGTTTGTGGTTGCGGAAATCATCATGACCGCCACTTTTACCCGATTTTCCGGTGCGGCAGTGAAGTACATCGCTAAAGAAATGCCCACGGCACCAAAGATTTTTGAGTTACCGTGCAGTGCAAAACCGCCTTCCGGGAACAACGATTTCAGCGGCTCGGCGCTCAAACTGAACTCTTGCAGATGCTGCGCCCAGTa                                                 <  1:144653/239‑1 (MQ=255)
                                                                                                                                                                                           gaaaGGTAAATCCCGGCGGTCCGTAAACCCCCCCCAGCGTGGGGTTTAGGGTGGCGGAAACCAAAAACCCCGCCACTTTTACCCGATTTTCCGGTGCGGCAGTGAAGTACATCGCTAAAGAAATGCCCGCGGCACCAAAGATTTTTGAGTTACCGTGCAGGGCAAAACCGCCTTCCGGGAACAACGATTTCAGCGGCTCGGCGCTCAAACTGAACTCTTGCAGATGCTGCGCCCAGTa                                                 <  2:16444/236‑1 (MQ=255)
                                                                                                                                                                                                      tCCAGCGGTTCGGTAATTCCCACCAGCATGGCGGTTAAGGTTGCGGGAATCAGCAAGCCCGCCACTTTTACCCGATTTTCCGGGGCGGCAGTGAAGTACATCGCTAAAGAAATGCCCGCGGCACCAAAGATTTTTTAGTTACCGTGCAGGGCAAAACCGCCTTCCGGGGACAAAGATTTTAGCGGCTCGGGGCCCAAAATGAACTCTTGCAGATGCTGCGCCCAGGACATCTGAATgc                                      >  1:16444/1‑238 (MQ=255)
                                                                                                                                                                                                                     atccccACCAGCATGGCGGTTAAGGTTGCGGGAACAAGCAAGCCCGCCACTTTTACCCGATTTTCCGGTGCGGCAGTGAAGTACATCGCTAAAGAAATGCCCACGGCACCAAAGATTTTTGAGTTACCGTGCAGGGCAAAACCGCCTTCCGGGAACAACGATTTCAGCGGCTCGGCGCTCAAACTGAACTCTTGCAGATGCTGCGCCCAGTACATCTGAATGCCGCCTTCAACAGCTGc                      <  1:615608/236‑1 (MQ=255)
                                                                                                                                                                                                                            cgcgaaTGGCGTTAAGGGTCGCGGAAATAAGAACGCCCGCCACTTTTACCCGATTTTCCGGTGCGGCAGTGAAGTACATCGCTAAAGAAATGCCCACGGCACCAAAGATTTTTGAGTTACCGTGCAGGGCAAAACCGCCTTCCGGGAACAACGATTTCAGCGGCTCGGCGCTCAAACTGAACTCTTGCAGATGCTGCGCCCAGTACATCTGAATGCCGCCTTCAACAGCTGCCGGACCa               <  1:327359/234‑1 (MQ=255)
                                                                                                                                                                                                                            ccacaagggCGGTAAGGGGTGCGGAAATCAGAAAGCCCGCCACTTTTACCCGATTTTCCGGTGCGGCAGTGAAGTACATCGCTAAAGAAATGCCCACGGCACCAAAGATTTTTGAGTTACCGTGCAGGGCAAAACCGCCTTCCGGGAACAACGATTTCAGCGGCTCGGCGCTCAAACTGAACTCTTGCAGATGCTGCGCCCAGTACATCTGAATGCCGCCTTCAACAGCTGCCGGACCa               <  1:549684/232‑1 (MQ=255)
                                                                                                                                                                                                                            ccaGCTGGGCGGTTAGGGTGGCGGGAACCAGCACGCCCGCCACTTTTACCCGATTTTCCGGTGCGGCAGTGAAGTACATCGCTAAAGAAATGCCCACGGCACCAAAGATTTTTGAGTTACCGTGCAGGGCAAAACCGCCTTCCGGGAACAACGATTTCAGCGGCTCGGCGCTCAAACTGAACTCTTGCAGATGCTGCGCCCAGTACATCTGAATGCCGCCTTCAACAGCTGCCGGACCa               <  2:992772/239‑1 (MQ=255)
                                                                                                                                                                                                                                     cGGTTAAGGTTGGGGAAATCAGCAAGCCCGCCACTTTTACCCGATTTTCCGGTGCGGCAGTGAAGTACATCGCTAAAGAAATGCCCACGGCACCAAAGATTTTTGAGTTACCGTGCAGGGCAAAACCGCCTTCCGGGAACAACGATTTCAGCGGCTCGGCGCTCAAACTGAACTCTTGCAGATGCTGCGCCCAGTACATCTGAATGCCGCCTTCAACAGCTGCCGGACCAAAGATaaa       <  1:1216924/238‑1 (MQ=255)
                                                                                                                                                                                                                                            tAAGGTTGGGGAATTCACAAAGCCCGCCACTTTTACCCGATTTTCCGGTGCGGCATTGAAGTACATCGCTAAAGAAATGCCCACGGCACCAAAGATTTTTGAGTTACCGTGCAGGGCAAAACCGCCTTCCGGGAACAACGATTTCAGCGGCTCGGCGCTCAAACTGAACTCTTGCAGATGCTGCGCCCAGTACATCTGAATGCCGCCTTCAACAGCTGCCGGACCAAAGATAAACTGTc  <  1:520416/239‑1 (MQ=255)
                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                           
AACCCAATGGCGATTTGGGTCAGCATCATATCCGCGTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACCTAGTCAATCAGACCTCCGCCCATGTTGCCCACCACACCAAAGAGATACATTACGGTCGACATTGAGGCCGCCAGCACGGCGTGTACCGCAAACAGCAACGGTGAAATGAACAGGAAGGTAAATTCCAGCGGTTCGGTAATTCCCACCAGCATGGCGGTTAAGGTTGCGGGAATCAGCAAGCCCGCCACTTTTACCCGATTTTCCGGTGCGGCAGTGAAGTACATCGCTAAAGAAATGCCCACGGCACCAAAGATTTTTGAGTTACCGTGCAGGGCAAAACCGCCTTCCGGGAACAACGATTTCAGCGGCTCGGCGCTCAAACTGAACTCTTGCAGATGCTGCGCCCAGTACATCTGAATGCCGCCTTCAACAGCTGCCGGACCAAAGATAAACTGTC  >  NC_000913/3862423‑3862894

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: