Predicted mutation | |||||||
---|---|---|---|---|---|---|---|
evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | NC_000913 | 2,014,202 | T→C | 16.5% | I325T (ATC→ACC) | fliF → | flagellar basal‑body MS‑ring and collar protein |
Read alignment evidence... | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 2,014,202 | 0 | T | C | 16.5% | 195.8 / 6.1 | 78 | I325T (ATC→ACC) | fliF | flagellar basal‑body MS‑ring and collar protein |
Reads supporting (aligned to +/- strand): ref base T (55/8); new base C (2/12); total (57/21) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 2.45e-07 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
GATCCTGTCGCCTATTGTTGGTAACGGTAATATTCACGCCCAGGTTACGGCGCAGCTGGACTTCGCCAGTAAAGAACAAACGGAAGAACAGTATCGCCCTAACGGTGATGAATCTCATGCGGCGCTT‑CGTTCACGCCAGCTTAATGAGAGCGAGCAAAGCGGTTCCGGTTATCCGGGCGGCGTACC‑GGGGGCGTTGTCGAATCAA‑CCGGCACCTGCG‑AATAACGCGCCAATCAGCACGCCTCCGGCAAATCAAAATAACCGCCAGCAGCAGGCGAGCACCACCAGCAATAGTGGGCCGCGTAGCACACAGCGGAATGAAACCAGTAACTACGAAGTCGATCGCACCATTCGTCATACCAAAATGAACGTGGGCGAT‑GTGCAACGTCTGTCAGTCGCGGTCGTGGTGAATTACAAAACCTTGCCAGATGGCAAACCGTTGCCTCTCAGCAACGAACAGATGAAGC > NC_000913/2013972‑2014435 | gATCCTGTCGCCTAATGTTGGTAACGGTAATATACACGCCCAGGTCACGGCGCAGCTGGACTTCGCCAGTAAAGAACAAACGGAAGAACAGTATCGCCCTAACGGTGATGAATCTCATGCGGCGCTT‑CGTTCACGCCAGCTTAATGAGAGCGAGCAAAGCGGTTCCGGTTATCCGGGCGGCGTACC‑GGGGGCGTTGTCGAATCAA‑CCGGCACCTGCG‑AATAACGCGCCAATCAGCAc < 2:323985/237‑1 (MQ=255) aTCCTGTCGCCTATTGTTGGTAACGGTAATATTCACGCCCAGGTTACGGCGCAGCTGGACTTCGCCAGTAAGGAACAAACGGAAGAACAGTATCGCCCTAACGGTGATGAATCTCATGCGGCGCTT‑CGTTCACGCCAGCTTAATGAGAGCGAGCAAAGCGGTTCCGGTTATCCGGGCGGCGTACC‑GGGGGCGTTGTCGAATCAA‑CCGGCACCTGCG‑AATAACGCGCCAATCAGCACg > 1:183418/1‑237 (MQ=255) aTCCTGTCGCCTATTGTTGGTAACGGTAATATTCACGCCCAGGTTACGGCGCAGCTGGACTTCGCCAGTAAAGAACAAACGGAAGAACAGTATCGCCCTAACGGTGATGAATCTCATGCGGCGCTT‑CGTTCACGCCAGCTTAATGAGAGCGAGCAAAGCGGTTCCGGTTATCCGGGCGGCGTACC‑GGGGGCGTTGTCGAATCAA‑CCGGCACCTGCG‑AATAACGCGCCAATCAGCACg > 1:548568/1‑237 (MQ=255) aTCCTGTCGCCTATTGTTGGTAACGGTAATATTCACGCCCAGGTTACGGCGCAGCTGGACTTCGCCAGTAAAGAACAAACGGAAGAACAGTATCGCCCTAACGGTGATGAATCTCATGCGGCGCTT‑CGTTCACGCCAGCTTAATGAGAGCGAGCAAAGCGGTTCCGGTTATCCGGGCGGCGTACC‑GGGGGCGTTGTCGAATCAA‑CCGGCACCTGCG‑AATAACGCGCCAATCAGCACGc > 1:993724/1‑238 (MQ=255) aTCCTGTCGCCTATTGTTGGTAACGGTAATATTCACGCCCAGGTTACGGCGCAGCTGGACTTCGCCAGTAAAGAACAAACGGAAGAACAGTATCGCCCTAACGGTGATGAATCTCATGCGGCGCTT‑CGTTCACGCCAGCTTAATGAGAGCGAGCAAAGCGGTTCCGGTTATCCGGGCGGCGTACC‑GGGGGCGTTGTCGAATCAA‑CCGGCACCTGCG‑AATAACGCGCCAATCAGCACGc > 1:1188859/1‑238 (MQ=255) tCGCCTATTGGTGGTAACGGTAATATTCACGCCCAGGTTACGGCGCAGCTGGACTTCGCCAGTAAAGAACAAACGGAAGAACAGTATCGCCCTAACGGTGATGAATCTCATGCGGCGCTT‑CGTTCACGCCAGCTTAATGAGAGCGAGCAAAGCGGTTCCGGTTATCCGGGCGGCGTACC‑GGGGGCGTTGTCGAATCAA‑CCGGCACCTGCG‑AATAACGCGCCAATCAGCACGCCTCCGGc < 1:998047/239‑1 (MQ=255) cGGTAATATTCACGCCCAGGTTACGGCGCAGCTGGACTTCGCCAGTAAAGAACAAACGGAAGAACAGTATCGCCCTAACGGTGATGAATCTCATGCGGCGCTT‑CGTTCACGCCAGCTTAATGAGAGCGAGCAAAGCGGTTCCGGTTATCCGGGCGGCGTACC‑GGGGGCGTTGTCGAATCAA‑CCGGCACCTGCG‑AATAACGCGCCAATCAGCACGCCTCCGGCACATCAAAATAACCGcc > 2:1178190/1‑239 (MQ=255) aCGCGCAGGTTACGGCGCAGCCGGACTTCGCCAGTAAAGAACAAACGGAAGAACAGTATCGCCCTAACGGTGCTGAATCTCATGCCGCGACT‑CGTTCACGCCAGCTTACTGAGAGCGAGCAAAGCGGTTCCGGTAATCCGGGCGGCGCACC‑GGGGGCGGTGGCGAAGCAC‑CCGACACCTCCG‑AGTAAGGCGCCAACCAGCAAGCCTCCGGCCAATCAAAATCCCCGCAAACAGCAAGCg > 2:877686/1‑239 (MQ=255) aCGCCCAGGTTACGGCGCAGCTGGACTTCGCCAGTAAAGAACAAACGGAAGAACAGTATCGCCCTAACGGTGATGAATCTCATGCGGCGCTT‑CGTTCACGCCAGCTTAATGAGAGCGAGCAAAGCGGTTCCGGTTATCCGGGCGGCGTACC‑GGGGGCGTTGTCGAATCAA‑CCGGCACCTGCG‑AATAACGCGCCAATCAGCACGCCTCCGGCAACTCAAAATAACCGCCAGCCGCCGGCg > 1:572454/1‑239 (MQ=255) aCGCCCAGGTTACGGCGCAGCTGGACTTCGCCAGTAAAGAACAAACGGAAGAACAGTATCGCCCTAACGGTGATGAATCTCATGCGGCGCTT‑CGTTCACGCCAGCTTAATGAGAGCGAGCAAAGCGGTTCCGGTTATCCGGGCGGCGTACC‑GGGGGCGTTGTCGAATCAA‑CCGGCACCTGCG‑AATAACGCGCCAATCAGCACGCCTCCGGCAAATCAAAATAACCGCCAGCAGCAGGCg > 1:1147159/1‑239 (MQ=255) aCGCCCAGGTTACGGCGCAGCTGGACTTCGCCAGTAAAGAACAAACGGAAGAACAGTATCGCCCTAACGGTGATGAATCTCATGCGGCGCTT‑CGTTCACGCCAGCTTAATGAGAGCGAGCAAAGCGGTTCCGGTTATCCGGGCGGCGTACC‑GGGGGCGTTGTCGAATCAA‑CCGGCACCTGCG‑AATAACGCGCCAATCAGCACGCCTCCGGCAAATCAAAATAACCGCCAGCAGCAGGCg > 1:628277/1‑239 (MQ=255) aCGCCCAGGTTACGGCGCAGCTGGACTTCGCCAGTAAAGAACAAACGGAAGAACAGTATCGCCCTAACGGTGATGAATCTCATGCGGCGCTT‑CGTTCACGCCAGCTTAATGAGAGCGAGCAAAGCGGTTCCGGTTATCCGGGCGGCGTACC‑GGGGGCGTTGTCGAATCAA‑CCGGCACCTGCG‑AATAACGCGCCAATCAGCACGCCTCCGGCAAATCAAAATAACCGCCAGCAGCAGACg > 2:150833/1‑239 (MQ=255) aCGCCCAGGTTACGGCGCAGCTGGACTTCGCCAGTAAAGAACAAACGGAAGAACAGTATCGCCCTAACGGTGATGAATCTCATGCGGCGCTT‑CGTTAACGCCAGCTTAATGAGAGCGAGCAAAGCGGTTCCGGTTATCCGGGCGGCGTACC‑GGGGGCGTTGTCGAATCAA‑CCGGCACCTGCG‑AATAACGCGCCAATCAGCACGCCTCCGGCAAATCAAACTCACCGCCAGCAGCCGGcc > 2:624868/1‑238 (MQ=255) gCCCAGGTTACGGCGCAGCTGGACTTCGCCAGTAAAGAACAAACGGAAGAACAGTATCGCCCTAACGGTGATGAATCTCATGCGGCGCTT‑CGTTCACGCCAGCTTAATGAGAGCGAGCAAAGCGGTTCCGGTTATCCGGGCGGCGTACC‑GGGGGCGTTGTCGAATCAA‑CCGGCACCTGCG‑AATAACGCGCCAATCAGCACGCCTCCGGCAAATCAAAATAACCGCCAGCAGCAGGCGa > 1:947274/1‑238 (MQ=255) ggTTACGGCGCAGCTGGACTTCGCCAGTAAAGAACAAACGGAAGAACAGTATCGCCCTAACGGTGATGAATCTCATGCGGCGCTT‑CGTTCACGCCAGCTTAATGAGAGCGAGCAAAGCGGTTCCGGTTATCCGGGCGGCGTACC‑GGGGGCGTTGTCGAATCAA‑CCGGCACCTGCG‑AATAACGCGCCAATCAGCCCGCCTCCGGCAAATAAAACTAACCGCCAGAAGCAGGCGAGcaca > 2:694416/1‑237 (MQ=255) ggTTACGGCGCAGCTGGACTTCGCCAGTAAAGAACAAACGGAAGAACAGTATCGCCCTAACGGTGATGAATCTCATGCGGCGCTT‑CGTTCACGCCAGCTTAATGAGAGCGAGCAAAGCGGTTCCGGTTATCCGGGCGGCGTACC‑GGGGGCGTTGTCGAATCAA‑CCGGCACCTGCG‑AATAACGCGCCAATCAGCACGCCTCCGGCAAATCAAAATAACCGCCAGCAGCAGGCGAGcacc > 1:678451/1‑238 (MQ=255) gTTACGGCGCAGCTGGACTTCGCCAGTAAAGAACAAACGGAAGAACAGTATCGCCCTAACGGTGATGAATCTCATGCGGCGCTT‑CGTTCACGCCAGCTTAATGAGAGCGAGCAAAGCGGTTCCGGTTATCCGGGCGGCGTACC‑GGGGGCGTTGTCGAATCAA‑CCGGCACCTGCG‑AATAACGCGCCAATCAGCACGCCTCCGGCAAATCAACATAACCGCCAGCAGCACGCCAGcacca > 2:200913/1‑238 (MQ=255) ttcgggCGCCTCTGGACTTCGCAAGTAAAGAACAACCGAGAACACAGTATCGCCCTAAGGGTGATAACTCCCTTGCGGCGCTT‑CGTCCCGGCCAGTTAAATGAGAGCGAGCAACGCGTTCCCGTTTGTCCGGGGGGCGTACG‑GGGGGCGTTGCCAAACCAC‑CCGGCCCCTGCA‑AATACCGCGCAACCCAGCACGCCCCCGACAAATAAAAATAACCGCCAGCAGCAGGCGAGcaccac < 2:945429/234‑1 (MQ=255) cGGCGCAGCTGGACTTCGCCAGTAAAGAACAAACGGAAGAACAGTATCGCCCTAACGGTGATGAATCTCATGCGGCGCTT‑CGTTCACGCCAGCTTAATGAGAGCGAGCAAAGCGGTTCCGGTTATCCGGGCGGCGTACC‑GGGGGCGTTGTCGAATCAA‑CCGGCACCTGCG‑AATAACGCGCCAATCGGCCCGCCTCCGGCAAATCAAAATAACCGCCAGCAGCAGGCGAGCACCACCAGc > 2:766977/1‑239 (MQ=255) cGGCGCAGCTGGACTTCGCCAGTAAAGAACAAACGGAAGAACAGTATCGCCCTAACGGTGATGAATCTCATGCGGCGCTT‑CGTTCACGCCAGCTTAATGAGAGCGAGCAAAGCGGTTCCGGTTATCCGGGCGGCGTACC‑GGGGGCGTTGTCGAATCAA‑CCGGCACCTGCG‑AATAACGCGCCAATCAGCACGCCTCCGGCAAATCAAAATAACCGCCAGCAGCAGGCGAGCACCACCAGc > 2:1175346/1‑239 (MQ=255) cGGCGCAGCTGGACTTCGCCAGTAAAGAACAAACGGAAGAACAGTATCGCCCTAACGGTGATGAATCTCATGCGGCGCTT‑CGTTCACGCCAGCTTAATGAGAGCGAGCAAAGCGGTTCCGGTTATCCGGGCGGCGTACC‑GGGGGCGTTGTCGAATCAA‑CCGGCACCTGCG‑AATAACGCGCCAATCAGCACGCCTCCGGCAAATCAAAATAACCGCCAGCAGCAGGCGAGCACCACCAGc > 1:528014/1‑239 (MQ=255) aGCTGGACTTCGCCAGTAAAGAACAAACGGAAGAACAGTATCGCCCTAACGGTGATGAATCTCATGCGGCGCTT‑CGTTCACGCCAGCTTAATGAGAGCGAGCAAAGCGGTTCCGGTTATCCGGGCGGCGTACC‑GGGGGCGTTGTCGAATCAA‑CCGGCACCTGCG‑AATAACGCGCCAATCAGCACGCCTCCGGCAAATCAAAATAACCGCCAGCAGCAGGCGAGCACCACCAGCAATAGt > 2:409228/1‑239 (MQ=255) aGCTGGACTTCCCCAGTAAAAAACAAACGGAAGAACTGTATCTCCCTAACGGAGATGAATCTCACGCGGAGCTT‑CGTTCACGTCAGCTTAATGAGCGCGAGCAAAGCGGTGCCGGTTATCCGGGCGGCGTACC‑GGGGGCGTTGTCGAATCAA‑CCGGCACCTGCG‑AATAACGCGCCAATCAGCACGCCTCCGGCAAATCAAACTAACAGCCAGCAGCAGGCGAGCACCACCCGCCTTAGt > 2:107902/1‑239 (MQ=255) ggACTGCGCCAGAAAAGAACAAAGGGAAGAAAAGCAGCCCCCAAAGGGTGATGAACCCCATGGGGCGCTT‑CGTCCACGCCAGCTAAAGGAGGGCGAGAAAAGCGTTCCCGGTTACCCGGGCGGCGTCCC‑GGGGGCTTTGTCAAACCAA‑CCGGCACCTGCG‑AATAACGCGCCAATCAGCACGCCCCCGGAAAATCAAAATAACCGCCAGCAGCAGGCGAGCACCACCAGCAATAGTggg < 2:993724/238‑1 (MQ=255) cTTCGCCAGTAAAGAACAAACGGAAGAACAGTATCGCCCTAACGGTGATGAATCTCATGCGGCGCTT‑CGTTCACGCCAGCTTAATGAGAGCGAGCAAAGCGGTTCCGGTTATCCGGGCGGCGTACC‑GGGGGCGTTGTCGAATCAA‑CCGGCACCTGCG‑AATAACGCGCCAATCAGCACGCCTCCGGCAAATCAAAATAACCGCCAGCAGCAGGCGCGCACCACCAGCAATAGTGGGCcgc > 1:451491/1‑239 (MQ=255) cGCCAGTAAAGAACAAACGGAAGAACAGTATCGCCCTAACGGTGATGAATCTCATGCGGCGCTT‑CGTTCACGCCAGCTTAATGAGAGCGAGCAAAGCGGTTCCGGTTATCCGGGCGGCGTACC‑GGGGGCGTTGTCGAATCAA‑CCGGCACCTGCG‑AATAACGCGCCAATCAGCACGCCTCCGGCAAATCAAAATAACCGCCAGCAGCAGGCGAGCACCACCAGCAATAGTGGGCCGCGTa > 1:345360/1‑239 (MQ=255) aaacaCAACCGGCAGACCGCTATCGCCTCACGGGTGCGGAATCCCAGGC‑GCGCTTCCGTCCGCACCCGCTTATGGAGGGCGGGCAAAGCGCTTCCGTTTCTCCGGACGGCGTCCC‑GGGGGCTGTGTCAAAACAA‑CCGGCACCTGCG‑AATACCGCGCAAACCAGCCCGCCCCCGGCAAATCAAAATATCCGCCAGCAGCAGGCGAGCACCACCAGCAATAGTGGGCCGCGTAGCACACAg < 2:1147159/235‑1 (MQ=255) aCGGAAGAACAGTATCGCCCTAACGGTGATGAATCTCATGCGGCGCTT‑CGTTCACGCCAGCTTAATGAGAGCGAGCAAAGCGGTTCCGGTTATCCGGGCGGCGTACC‑GGGGGCGTTGTCGAATCAA‑CCGGCACCTGCG‑AATAACGCGCCAATCAGCACGCCTCCGGCAAATCAAAATAACCGCCAGCAGCAGGCGAGCACCACCAGCAATAGTGGGCCGCGTAGCACACAGCGGAATGa > 1:92983/1‑239 (MQ=255) agaaCAGTATCGCCCTAACGGTGATGAATCTCATGCGGCGCTT‑CGTTCACGCCAGCTTAATGAGAGCGAGCAAAGCGGTTCCGGTTATCCGGGCGGCGTACC‑GGGGGCGTTGTCGAATCAA‑CCGGCACCTGCG‑AATAACGCGCCAATCAGCACGCCTCCGGCAAATCAAAATAACCGCCAGCAGCAGGCGAGCACCACCAGCAATAGGGGGCCGCGAAGCCCACAGCGGAATGAAACCa > 2:317751/1‑239 (MQ=255) tagcGCCCTAACGGTGATGAATCTCATGCGGCGCTT‑CGTTCCCGCCAGCTTAAGGAGGGCGAGAAAAGGGTTTCCGGTTACCGGGGCGGCGTCCC‑GGGGGCGTTGTCGAATCAA‑CCGGCCCCTGCG‑AATAACGCGCCAATCAGCCCGCCTCGGGAAAATCAAAATAACCGCCAGCAGCAGGCGAg < 1:197700/182‑1 (MQ=255) tATCGCCCTAACGGTGATGAATCTCATGCGGCGCTT‑CGTTCACGCCAGCTTAATGAGAGCGAGCAAAGCGGTTCCGGTTATCCGGGCGGCGTACC‑GGGGGCGTTGTCGAATCAA‑CCGGCACCTGCG‑AATAACGCGCCAATCAGCACGCCTCCGGCAAATCAAAATAACCGCCAGCAGCAGGCGAGCACCACCAGCAATAGTGGGCCGCGTAGCACACAGCGGAATGAAACCAGTAACt > 1:95919/1‑238 (MQ=255) tCGCCCTAACGGTGATGAATCTCATGCGGCGCTT‑CGTTCACGCCAGCTTAATGAGAGCGAGCAAAGCGGTTCCGGTTATCCGGGCGGCGTACC‑GGGGGCGTTGTCGAATCAA‑CCGGCACCTGCG‑AATAACGCGCCAATCAGCACGCCTCCGGCAAATCAAAATACCCGCCAGCAGCAGGCGAGCACCACCAGCAATATGGGGCCGCGTACCACAAAGCGGAGTGACACCACAACCTACg > 2:636580/1‑239 (MQ=255) tCGCCCTAACGGTGATGAATCTCATGCGGCGCTT‑CGTTCACGCCAGCTTAATGAGAGCGAGCAAAGCGGTTCCGGTTATCCGGGCGGCGTACC‑GGGGGCGTTGTCGAATCAA‑CCGGCACCTGCG‑AATAACGCGCCAATCAGCACGCCTCCGGCAAATCAAAATAACCGCCAGCAGCAGGCGAGCACCACCAGCAATAGTGGGCCGCGTAGCACACAGCGGAATGAAACCAGTAACTACg > 2:238213/1‑239 (MQ=255) tCGCCCTAACGGTGATGAATCTCATGCGGCGCTT‑CGTTCACGCCAGCTTAATGAGAGCGAGCAAAGCGGTTCCGGTTATCCGGGCGGCGTACC‑GGGGGCGTTGTCGAATCAA‑CCGGCACCTGCG‑AATAACGCGCCAATCAGCACGCCTCCGGCAAATCAAAATAACCGCCAGCAGCAGGCGAGCACCACCAGCAATAGTGGGCCGCGTAGCACACAGCGGAATGAAACCAGTAACTACg > 1:605281/1‑239 (MQ=255) cccTAACGGTGATGAATCTCATGCGGCGCTT‑CGTTCACGCCAGCTTAATGAGAGCGAGCAAAGCGGTTCCGGTTATCCGGGCGGCGTACC‑GGGGGCGTTGTCGAATCAA‑CCGGCACCTGCG‑AATAACGCGCCAATCAGCACGCCTCCGGCAAATCAAAATAACCGCCAGCAGCAGGCGAGCACCACCAGCAATAGTGGGCCGCGTAGCACACAGCGGAATGAAACCAGTAACTACGAAg > 1:817158/1‑239 (MQ=255) cccTAACGGTGATGAATCTCATGCGGCGCTT‑CGTTCACGCCAGCTTAATGAGAGCGAGCAAAGCGGTTCCGGTTATCCGGGCGGCGTACC‑GGGGGCGTTGTCGAATCAA‑CCGGCACCTGCG‑AATAACGCGCCAATCAGCACGCCTCCGGCAAATCAAAATAACCGCCAGCAGCAGGCGAGCACCACCAGCAATAGTGGGCCGCGTAGCACACAGCGGAATGAAACCAGTAACTACGAAg > 1:558376/1‑239 (MQ=255) aCGGTGATGAATCTCATGCGGCGCTT‑CGTTCACGCCAGCTTAATGAGAGCGAGCAAAGCGGTTCCGGTTATCCGGGCGGCGTACC‑GGGGGCGTTGTCGAATCAA‑CCGGCACCTGCG‑AATAACGCGCCAATCAGCACGCCTCCGGCAAATCAAAATAACCGCCAGCAGCAGGCGAGCACCACCAGc > 2:197700/1‑185 (MQ=255) aCGGTGATGAATCTCATGCGGCGCTT‑CGTTCACGCCAGCTTAATGAGAGCGAGCAAAGCGGTTCCGGTTATCCGGGCGGCGTACC‑GGGGGCGTTGTCGAATCAA‑CCGGCACCTGCG‑AATAACGCGCCAATCAGCACGCCTCCGGCAAATCAAAATAACCGCCAGCAGCAGGCGAGCACCACCAGCAATAGTGGGCCGCGTAGCACACAGCGGAATGAAACCAGTAACTACGAAGTCGa > 1:824077/1‑238 (MQ=255) aCGGTGATGAATCTCATGCGGCGCTT‑CGTTCACGCCAGCTTAATGAGAGCGAGCAAAGCGGTTCCGGTTATCCGGGCGGCGTACC‑GGGGGCGTTGTCGAATCAA‑CCGGCACCTGCG‑AATAACGCGCCAATCAGCACGCCTCCGGCAAATCAAAATAACCGCCAGCAGCAGGCGAGCACCACCAGCAATAGTGGGCCGCGTAGCACACAGCGGAATGAAACCAGTAACTACGAAGTCGa > 1:965458/1‑238 (MQ=255) atgaATCTCATGCGGCGCTT‑CGTTCACGCCAGCTTAATGAGAGCGAGCAAAGCGGTTCCGGTTATCCGGGCGGCGTACC‑GGGGGCGTTGTCGAATCAA‑CCGGCACCTGCG‑AATAACGCGCCAATCAGCACGCCTCCGGCAAATCAAAATAACCGCCAGCAGCAGGCGAGCACCACCAGCAATAGTGGGCCGCGTAGCACACAGCGGAATGAAACCAGTAACTACGAAg > 1:1189376/1‑228 (MQ=255) atgaATCTCATGCGGCGCTT‑CGTTCACGCCAGCTTAATGAGAGCGAGCAAAGCGGTTCCGGTTATCCGGGCGGCGTACC‑GGGGGCGTTGTCGAATCAA‑CCGGCACCTGCG‑AATAACGCGCCAATCAGCACGCCTCCGGCAAATCAAAATAACCGCCAGCAGCAGGCGAGCACCACCAGCAATAGTGGGCCGCGTAGCACACAGCGGAATGAAACCAGTAACTACGAAGTCGATCGCAcc > 1:368425/1‑239 (MQ=255) atgaATCTCATGCGGCGCTT‑CGTTCACGCCAGCTTAATGAGAGCGAGCAAAGCGGTTCCGGTTATCCGGGCGGCGTACC‑GGGGGCGTTGTCGAATCAA‑CCGGCACCTGCG‑AATAACGCGCCAATCAGCACGCCTCCGGCAAATCAAAATAACCGCCAGCAGCAGGCGAGCACCACCAGCAATAGTGGGCCGCGTAGCACACAGCGGAATGAAACCAGTAACTACGAAGTCGATCGCAc > 2:431717/1‑238 (MQ=255) atgaATCTCATGCGGCGCTT‑CGTTCACGCCAGCTTAATGAGAGCGAGCAAAGCGGTTCCGGTTATCCGGGCGGCGTACC‑GGGGGCGTTGTCGAATCAA‑CCGGCACCTGCG‑AATAACGCGCCAATCAGCACGCCTCCGGCAAATCAAAATAACCGCCAGCAGCAGGCGAGCACCACCAGCAATAGTGGGCCGCGTAGCACACAGCGGAATGAAACCAGTAACTACGAAGCCGATCGCAcc > 1:302192/1‑239 (MQ=255) atgaATCCCATGCGGCGCTT‑CGTTCACGCCAGCTTAATGAGAGCGAGCAAAGCGGTTCCGGTTATCCGGGCGGCGTACC‑GGGGGCGTTGTCGAATCAA‑CCGGCACCTGCG‑AATAACGCGCCAATCAGCACGCCTCCGGCAAATCAAAATAACCGCCAGCAGCAGGCGAGCACCACCAGCAATAGTGGGCCGCGCAGCACACAGCGGAATGAAACCAGTAACTACGAAGTCGATCGCAc > 2:1023006/1‑238 (MQ=255) cATGCGGCGCTT‑CGTTCACGCCAGCTTAATGAGAGCGAGCAAAGCGGTTCCGGTTATCCGGGCGGCGTACC‑GGGGGCGTTGTCGAATCAA‑CCGGCACCTGCG‑AATAACGCGCCAATCAGCACGCCTCCGGCAAATCAAAATAACCGCCAGCAGCAGGCGAGCACCACCAGCAATAGTGGGCCGCGTAGCACACAGCGGAATGAAACCAGTAACTACGAAGTCGATCGCACCATTCGTCa > 2:283880/1‑239 (MQ=255) tGCGGCGCTT‑CGTTCACGCCAGCTTAATGAGAGCGAGCAAAGCGGTTCCGGTTATCCGGGCGGCGTACC‑GGGGGCGTTGTCGAATCAA‑CCGGCACCTGCG‑AATAACGCGCCAATCAGCACGCCTCCGGCAAATCAAAATAACCGCCAGCAGCAGGCGAGCACCACCAGCAATAGTGGGCCGCGTAGCACACAGCGGAATGAAACCAGTAACTACGAAGTCGATCGCACCATTCGTCAt > 1:1021258/1‑238 (MQ=255) cggctCTT‑CGTACCCGCCACAGTAGGGAGGGAGAGCAAAGCGGTTCCGGTTGAGCGGGCGGCTCGCG‑GGGGGCGTTGCGTAATCAA‑CCGCCAGCGCCC‑AAAAGCGCACAAACCACCACCCCTCGGGAAAATCAAAATCACCGCCAGCAGCAGGCGAGCACCACCAGCAATAGTGGGCCGCGTAGCACACAGCGGAATGAAACCATTAACTACGAAGTCGATCGCACCATTCGTCATa < 2:1188859/237‑1 (MQ=255) gcTT‑CGTTCACGCCAGCTTAATGAGAGCGAGCAAAGCGGTTCCGTTTACCCGGGCGGC‑TACCGGGGGGCGTTGTCGAATCAA‑CCGGCACCTGCG‑AATAACGCGCCAACCAGCACGCCTCCGGAAAATCAAAAAAACCGCCAGCAGCAGGCGAGCACCACCAGCAATAGTGGGCCGCGTAGCACACAGCGGAATGAAACCAGTAACTACGAAGTCGATCGCACCATTCGTCATACCaaaa < 1:317751/239‑1 (MQ=255) cGCCAGCGTAAGGAGAGCAGCCAAAGCGGCCCCGCTTCTCCGGGCGGCGCACC‑GGTGGCTTTGACGAACCAA‑CCGCCACCTGCA‑AACAACCCCCCAATCAGCCCCCCCCGGGCAAATCAAAATAACCGCCAGCAGCAGGCGAGCACCACCAGCAATAGTGGGCCGCGTAGCACACAGCGGAATGAAACCAGTAACTACGAAGTCGATCGCACCATTCGTCATACCAAAATGAACGTggg < 2:532453/239‑1 (MQ=255) cTTAATGAGAGCGAGCAAAGCGGTTCCGGTTATCCGGGCGGCGTACC‑GGGGGCGTTGTCGAATCAA‑CCGGCACCTGCG‑AATAACGCGCCAATCAGCACGCCTCCGGCATATCAAAATAACCGCCAGCAGCAGGCGAGCACCACCAGCAATAGTGGGCCGCGTAGCACACAGCGGAATGAAACCAGTAACTACGAAGTCGATCGCACCATTCGTCATACCAAAATGAACGTGGGCGAt‑gt > 1:29374/1‑239 (MQ=255) gcgagcAAAGCGGTTCCGGTTATCCGGGCGGCGTACC‑GGGGGCGTTGTCGAATCAA‑CCGGCACCTGCG‑AATAACGCGCCAATCAGCACGCCTCCGGCAAATCAAAATAACCGCCAGCAGCAGGCGAGCACCACCAGCAATAGTGGGCCGCGTAGCACACAGCGGAATGAAACCAGTAACTACGAAGTCGATCGCACCATTCGCCATACCACAATGAAAGTGGGCGAT‑GTGCAACGTCTg > 2:734030/1‑239 (MQ=255) cgagcAAAGCGGTTCCGGTTATCCGGGCGGCGTACC‑GGGGGCGTTGTCGAATCAA‑CCGGCACCTGCG‑AATAACGCGCCAATCAGCACGCCTCCGGCAAATCAAAATAACCGCCAGCAGCAGGCGAGCACCACCAGCAATAGTGGGCCGCGTAGCACACAGCGGAATGAAACCAGTAACTACGAAGTCGATCGCACCATTCGTCATACCAAAATGAACGTGGGCGAT‑GTGCAACg > 1:239564/1‑234 (MQ=255) agcAAAGCGGTTCCGGTTATCCGGGCGGCGTACC‑GGGGGCGTTGTCGAATCAA‑CCGGCACCTGCG‑AATAACGCGCCAATCAGCACGCCTCCGGCAAATCAAAATAACCGCCAGCAGCAGGCGAGCACCACCAGCAATAGTGGGCCGCGTAGCACACAGCGGAATGAAACCAGTAACTACGAAGTCGATCGCACCATTCGTCATACCAAAATGAACGTGGGCGAT‑GTGCAACGTCTGTCa > 1:663257/1‑239 (MQ=255) gcAAAGCGGTTCCGGTTATCCGGGCGGCGTACC‑GGGGGCGTTGTCGAATCAA‑CCGGCACCTGCG‑AATAACGCGCCAATCAGCACGCCTCCGGCAAATCAAAATAACCGCCAGCAGCAGGCCAGCACCACCAGCACTAGTGGGCCGCGAAGCACACAGCGGAATGAAACCAGTAACTACGAAGTCGATCTCACCATTCGTCATACCAAAATAAACGCGGGCGAC‑GTGAAACGTcgctc > 2:628681/1‑233 (MQ=255) aGCGGTTCCGGTTATCCGGGCGGCGTACC‑GGGGGCGTTGTCGAATCAA‑CCGGCACCTGCG‑AATAACGCGCCAATCAGCACGCCTCCGGCAAATCAAAATAACCGCCAGCAGCAGGCGAGCACCACCAGCAATAGTGGGCCGCGTAGCACACAGCGGAATGAAACCAGTAACTACGAAGTCGATCGCACCATTCGTCATACCAAAATGAACGTGGGCGAT‑GTGCAACGTCTGTCAGTcgc > 1:1006191/1‑239 (MQ=255) gCGGTTCCGGTTATCCTGGCGGCGTACC‑GGGGGCGTTGTCGAATCAA‑CCGGCACCTGCG‑AATAACGCGCCAGTCAGCACGCCTCCGGCAAATCAAAATAACCGCCAGCGGCAGGGGGGCACCCCCACCAATAGTGGGCCGCGTCGCACACAGCGGAATCAAACCAGAAACTACGGCGGCGATGGCCCCACTCGTCATACCACAACGAACGTGGGCTAT‑GGGCAACGCCTGTCAGttgag > 2:215902/1‑235 (MQ=255) gCGGTTCCGGTTATCCGGGCGCCGTACC‑GGGGGCGTTGACGAAACAA‑CCGGCACCTGCG‑AATAACGCGCCAACCAGCACGCCTCCGACAACTCAAAATAACCGCCAGCAGCAGGCGAACCCCACCAGCTATAGTGGGCCACGTAGCACACGGCGGACTGAAACCCGTAACAACAAAGTCGATCCCACCATTCGTCATACCAAAATGAACGTCACCGATGGTGC‑ACGTCTGTCAgccacg > 2:908492/1‑234 (MQ=255) gccGGTTACCCGGGGGGCGCGCC‑GGGGGCGTGGCCAAAACAA‑CCGACAGCTGCG‑AATAACGCGCCAACCAGCACGCCTCCGGAAAACAAAAAAAACCGCCAGCAGCAGGCGAGCACCACCAGCAATAGTGGGCCGCGTAGCACACAGCGGAATGAAACCAGTAACTACGAAGTCGATCGCACCATTCGGCATACCAAAATGAACGTGGGCGAT‑GTGCAACGTCTGTCAGTCGCGGTCg < 2:1006191/237‑1 (MQ=255) ttacggggggggCGCACC‑GGGGGCGTTGTCGAATCAA‑CCGCCACCTGGG‑AAGAACGCGCCAACCGGCACGCCTCCGGAAAATCAAAAAAACCGCCAGCAGCAGGCGAGCACCACCAGCAATAGTGGGCCGCGTAACACACAGCGGAATGAAACCAGTAACTACGAAGTCGATCGCGCCATTCGTCATACCAAAATGAACGTGGGCGAT‑GTGCAACGTCTGTCAGTCGCGGTCgtggtg < 1:745/232‑1 (MQ=255) ctggccggggggcgtccg‑gggggcgtTG‑CGCAACAACCCGCCACCCGCC‑AACACCCGGCAACGCACCACGCCCCGGAAAAATAAAAATAACCGCCAGCAGCAGGCGAGCACCACCAGCAATAGTGGGCCGCGTAGCACACAGCGGAATGAAACCAGTAACTACGAAGTCGATCGCACCATTCGTCATACCAAAATGAACGTGGGCGAT‑GTGCAACGTCTGTCAGTCGCGGTCgtggtg < 2:92983/234‑1 (MQ=255) cGGGGGGCGGCCC‑GGGGGCGTGTGCGAACCAC‑CCGACACCTCGG‑AATAACGCGCCAATCAGCACGCCCCCGGAAAATCAAAATAACCGCCAGCAGCAGGCGAGCACCACCAGCAATAGTGGGCCGCGTAGCACACAGCGGAATGAAACCAGTAACTACGAAGTCGATCGCACCATTCGTCATACCAAAATGAACGTGGGCGAT‑GTGCAACGTCTGTCAGTCGCGGTCGTGGTGAATTAc < 2:1110410/239‑1 (MQ=255) cGGGCGCCGTACC‑GGGGGCGTTGTCGAACCAC‑CCGGCACCTGCA‑AATAACGCGCAAATCAGCACGCCTCCGGAAAATAAAAATAACCGCCAGCAGCAGGCGAGCACCACCAGCAATAGTGGGCCGCGTAGCACACAGCGGAATGAAACCAGTAACTACGAAGTCGATCGCACCATTCGTCATACCAAAATGAACGTGGGCGAT‑GTGCAACGTCTGTCAGTCGCGGTCGTGGTGAATTAc < 1:176946/239‑1 (MQ=255) gggggcgTACG‑GGGGGGGTTGTCGAATCAC‑CCGGCACCGGAG‑AATAACGCGCCAACCACCCCCCCCCCGGAAAATCAAAATAACCGCCAGCAGCAGGCGAGCACCACCAGCAATAGTGGGCCGCGTAGCACACAGCGGAATGAAACCAGTAACTACGAAGTCGATCGCACCATTCGTCATACCAAAATGAACGTGGGCGAT‑GTGCAACGTCTGTCAGTCGCGGTCGTGGTGAATTACa < 1:150833/235‑1 (MQ=255) gcggcgTACC‑GGGGGCGTTGTCGAATCAA‑CCGGCACCTGCG‑AATAACGCGCCAATCAGCACGCCTCCGGCAAATCAAAATAACCGCCAGCAGCAGGCGAGCACCACCAGCAATAGTGGGCCGCGTAGCACACAGCGGAATGAAACCAGTAACTACGAAGTCGATCGCACCATTCGTCATACCAAAATGAACGTGGGCGAT‑GTGCAACGTCTGTCAGTCGCGGTCGTGGTGAATTACaaa > 1:1119025/1‑239 (MQ=255) cgggcaccc‑GGGGGCGTGGCCGAACCAA‑CGGGCACC‑GCGCAATAAGGCGCCAACCAGCACCCCTCGGGAAAATCAAAAAAACCGCCAGCAGCAGGCGAGCACCACCAGCAATAGTGGGCCGCGTAGCACACAGCGGAATGAAACCAGTAACTACGAAGTCGATCGCACCATTCGTCATACCAAAATGAACGTGGGCGAT‑GTGCAACGTCTGTCAGTCGCGGTCGTGGTGAATTACaaa < 2:963507/231‑1 (MQ=255) gTACC‑GGGGGCGTTGTCGAATCAA‑CCGGCACCTGCG‑ACTAACGCGCCAATCAGCACGCCTCCGGCAAATCAAAATAACCGCCAGCAGCAGGCGAGCACCACCAGCAATAGTGGGCCGCGTAGCACACAGCGGCATGAAACCAGTAACTACGAAGTCGATCGCACCATTCGTCATACCAAAATGAACGTGGCCGAT‑GTGCAACGTCTGTCAGTCGCGGTCGTGGTGAATTAAAAAACCtt > 2:881665/1‑239 (MQ=255) cc‑GGGGGCGTTGCCGAATCAC‑CCGGCCCCTGCG‑AATACCGCGCAACTCAGCCCCCCCCGGGAAAAAAAAAATAACCGCCAGCAGCAGGCGAGCACCACCAGCAATAGTGGGCCGCGTAGCACACAGCGGAATGAAACCAGTAACTACGAAGTCGATCGCACCATTCGTCATACCAAAATGAACGTGGGCGAT‑GTGCAACGTCTGTCAGTCGCGGTCGTGGTGAATTACAAAACCTTGcc < 2:817158/239‑1 (MQ=255) gCGTTGTCGAATCAA‑CCGGCACCTGCG‑AATAACGCGCCAATCAGCACGCCTCCGGCAAATCAAAATAACCGCCAGCAGCAGGCGAGCACCACCAGCAATAGTGGGCCGCGTAGCACACAGCGGAATGAAACCAGTAACTACGAAGTCGATCGCACCATTCGTCATACCAAAATGAACGTGGGCGAT‑GTGCAACGTCTGTCAGTCGCGGTCGTGGGGAATTACAAAACCTTACCAGATg > 1:679995/1‑238 (MQ=255) cttGTCCACTAAA‑CCGGCACCTGAC‑AATAACGCCCGTACCACCCCGCCCAGGGCAAAGAAAAATGACCGCCAGCAGCAGGCGAGCACCACCAGCAATAGTGGGCCGCGTAGCACACAGCGGAATGAAACCAGTAACTACGAAGTCGATCGCGCCATTCGTCATACCAAAATGAACGTGGGCGAT‑GTGCAACGTCTGTCAGTCGCGGTCGTGGTGAATTACAAAACCTCGCCAGATGGc < 2:346437/237‑1 (MQ=255) ttGTCGAATCAA‑CCGGCACCTGCG‑AATAACGCGCCAATCAGCACGCCTCCGGCAAATCAAAATAACCGCCAGCAGCAGGCGAGCACCACCAGCAATAGTGGGCCGCGTAGCACACAGCGGAATGAAACCAGTAACTACGAAGTCGATCGCACCATTCGTCATACCAAAATGAACGTGGGCGAT‑GTGCAACGTCTGTCAGTCGCGGTCGTGGTGAATTACAAAACCTTGCCAGATGGCaa > 1:674177/1‑239 (MQ=255) tGTCGAATCAA‑CCGGCACCTGCG‑AATAACGCGCCAATCAGCACGCCTCCGGCAAATCAAAATAACCGCCAGCAGCAGGCGAGCACCACCAGCAATAGTGGGCCGCGTAGCACACAGCGGAATGAAACCAGTAACTACGAAGTCGATCGCACCATTCGTCATACCACAATGAACGTGGCCGAT‑GTGCAACGTCTCCCAGTCGCGGTCGCGGGGAATTACAAAACCCTGCCAGATGGcca > 2:535514/1‑236 (MQ=255) gaccGAAACAC‑CCGGCACGTGCG‑AAGAACGCGCAAACAAGCCCGCCTCGGGCAAATAAAAAAAACCGCCAGCAGCAGGCGAGCACCACCAGCAATAGTGGGCCGCGTAGCACACAGCGGAATGAAACCAGTAACTACGAAGTCGATCGCACCATTCGTCATACCAAAATGAACGTGGGCGAT‑GTGCAACGTCTGTCAGTCGCGGTCGTGGTGAATTACAAAACCTTGCCAGATGGCaa < 2:239564/235‑1 (MQ=255) aaa‑CCGGCCCCTGCG‑AACAACGCGCAAACCACCACCCCCCCGAAAAATCAAAATAACCGCCAGCAGCAGGCGAGCACCACCAGCAATAGTGGGCCGCGTAGCACACAGCGGAATGAAACCAGTAACTACGAAGTCGATCGCACCATTCGTCATACCAAAATGAACGTGGGCGAT‑GTGCAACGTCTGTCAGTCGCGGTCGTGGTGAATTACAAAACCTTGCCAGATGGCAAACCGTTGcc < 2:368425/238‑1 (MQ=255) gaacaaccggacAACCAGCACCCCCCAGGCAAATGAAAAAAACCGCCAGCAGCAGGCGAGCACCACCAGCAATAGTGGGCCGCGTAGCACACAGCGGAATGAAACCAGTAACTACGAAGTCGATCGCACCATTCGTCATACCAAAATGAACGTGGGCGAT‑GTGCAACGTCTGTCAGTCGCGGTCGTGGTGAATTACAAAACCTTGCCAGATGGCAAACCGTTGCCTCTCAGCAACGaa < 2:309339/227‑1 (MQ=255) cgcgCCAATCAGCACGCCTCCGGCAAATCAAAATAACCGCCAGCAGCAGGCGAGCACCACCAGCAATAGTGGGCCGCGTAGCACACAGCGGAATGAAACCAGTAACTACGAAGTCGATCGCACCATTCGTCATACCAAAATGAACGTGGGCGAT‑GTGCAACGTCTGTCAGTCGCGGTCGTTGTGAATTACAAAACCTTGCCAGATGGCAAACCGTTGCCTCTCAGCAACGAACAGATGa > 1:404497/1‑239 (MQ=255) cgcgCCAATCAGCACGCCTCCGGCAAATCAAAATAACCGCCAGCAGCAGGCGAGCACCACCAGCAATAGTGGGCCGCGTAGCACACAGCGGAATGAAACCAGTAACTACGAAGTCGATCGCACCATTCGTCATACCAAAATGAACGTGGGCGAT‑GTGCAACGTCTGTCAGTCGCGGTCGTGGTGAATTACAAAACCTTGCCAGATGGCAAACCGTTGCCTCTCAGCAACGAACAGATGa > 1:179193/1‑239 (MQ=255) cgcgCCAATCAGCACGCCTCCGGCAAATCAAAATAACCGCCAGCAGCAGGCGAGCACCACCAGCAATAGTGGGCCGCGTAGCACACAGCGGAATGAAACCAGTAACTACGAAGTCGATCGCACCATTCGTCATACCAAAATGAACGTGGGCGAT‑GTGCAACGTCTGTCAGTCGCGGTCGTGGTGAATTACAAAACCTTGCCAGATGGCAAACCGTTGCCTCTCAGCAACGAACAGATGa > 2:932773/1‑239 (MQ=255) gcCAATCAGCACGCCTCCGGCAAATCAAAATAACCGCCAGCAGCAGGCGAGCACCACCAGCAATAGTGGGCCGCGTAGCACACAGCGGAATGAAACCAGTAACTACGAAGTCGATCGCACCATTCGTCATACCAAAATGAACGTGGGCGAT‑GTGCAACGTCTGTCAGTCGCGGTCGTGGTGAATTACAAAACCTTGCCAGATGGCAAACCGTTGCCTCTCAGCAACGACCAGATGAAGc > 1:1160710/1‑239 (MQ=255) | GATCCTGTCGCCTATTGTTGGTAACGGTAATATTCACGCCCAGGTTACGGCGCAGCTGGACTTCGCCAGTAAAGAACAAACGGAAGAACAGTATCGCCCTAACGGTGATGAATCTCATGCGGCGCTT‑CGTTCACGCCAGCTTAATGAGAGCGAGCAAAGCGGTTCCGGTTATCCGGGCGGCGTACC‑GGGGGCGTTGTCGAATCAA‑CCGGCACCTGCG‑AATAACGCGCCAATCAGCACGCCTCCGGCAAATCAAAATAACCGCCAGCAGCAGGCGAGCACCACCAGCAATAGTGGGCCGCGTAGCACACAGCGGAATGAAACCAGTAACTACGAAGTCGATCGCACCATTCGTCATACCAAAATGAACGTGGGCGAT‑GTGCAACGTCTGTCAGTCGCGGTCGTGGTGAATTACAAAACCTTGCCAGATGGCAAACCGTTGCCTCTCAGCAACGAACAGATGAAGC > NC_000913/2013972‑2014435 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |