Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	2,459,225	T→C	24.5%	G415G (GGA→GGG)	fadI ←	beta‑ketoacyl‑CoA thiolase, anaerobic, subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,459,225	0	T	C	24.5%	177.1 / 12.3	96	G415G (GGA→GGG)	fadI	beta‑ketoacyl‑CoA thiolase, anaerobic, subunit
Reads supporting (aligned to +/- strand):  ref base T (45/25);  new base C (2/24);  total (47/49)
Fisher's exact test for biased strand distribution p-value = 4.44e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGTACGTCGATGGTG‑ATAACGGCAATGTTGTCCAGACGAACATTAAGGGTAAACGCTGATGTCATTTCCATTATTCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGTAACTAAACCAAAT‑CCACCGCCGCGACGGCGAAGTTCATGCAATGTCTGGGTAATCATCCGCGCGCCGGTCGCCGCGAAGGGATGCCCGTAAGCAATCGAACCGCCAAGCACGTTAAATTTGCTATCGTCCACTTCGCCAGTGGCATGTGCACGCCCCAGTGCTTCACGAGCAAAACGTTCACTACCCAGCAACTGAATATTCGCCAGCGTCTGAGCTGCAAAGGCTTCGTGCATATCGATCAATGTCAGA  >  NC_000913/2458996‑2459462                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                               acGCCTCGCAACTCTTTTTTTGCACGGAGTTGCTTAATAATGGCGCGCCCCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGTACGTCGATGGTG‑ATAACGGCAATGTTGTCCAGCCGAACATTAAGGGTAAACGCTGATGTCATTTCCATTATTCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGTAACTAAACAAAAT‑CCAccgccg                                                                                                                                                                                                                                      <  2:1166342/239‑1 (MQ=255)  cGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGTACGTCGATGGTG‑ATAACGGCAATGTTGTCCAGACGAACATTAAGGGTAAACGCTGATGTCATTTCCATTATTCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGTAACTAAACCAAAT‑CCACcgccgc                                                                                                                                                                                                                                     >  1:936814/1‑239 (MQ=255)    ccTCGCAACTCTTTTTTTTCACGGAGTTGCTTAATAATGGCGCGCCCCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGTACGTCGATGGGG‑ATAACGGCCATGTTATCCACACGAACCTTAAGGGTAAACGCTGATGTCCTTTCCATTATTCCGCCTCCAGAACCATTGCCGCGCCAAGCCCCCCGGCAGACCAGGCGTTCACTAAACCCAAC‑CACCcgccgct                                                                                                                                                                                                                                    >  2:1005599/1‑237 (MQ=255)      tCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGTACGTCGATGGTG‑ATAACGGCAATGTTGTCCAGACGAACATTAAGGGTAAACGCTGATGTCATTTCCATTATTCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGTAACTAAACCAAAT‑CCACCCCCGCGACg                                                                                                                                                                                                                                 >  1:1125153/1‑239 (MQ=255)          aaCTCTTTGTTTTCACGGATTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGTACGTCGATGGTG‑ATAACGGCAATGTTGTCCAGACGAACATTAAGGGTAAACGCTGATGTCATTTCCATTATTCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGTAACTAAACCAAAT‑CCACCGCCGCGACGGCGa                                                                                                                                                                                                                             <  1:1054005/239‑1 (MQ=255)          aaCTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGTACGTCGATGGTG‑ATAACGGCAATGTTGTCCAGACGAACATTAAGGGTAAACGCTGATGTCATTTCCATTATTCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGTAACTAAACCAAAT‑CCACCGCCGCGACGGCGa                                                                                                                                                                                                                             <  1:176047/239‑1 (MQ=255)               tttGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGTACGTCGATGGTG‑ATAACGGCAATGTTGTCCAGACGAACATTAAGGGTAAACGCTGATGTCATTTCCATTATTCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGTAACTAAACCAAAT‑CCACCGCCGCGACGGCGGAGTTc                                                                                                                                                                                                                        >  1:957921/1‑239 (MQ=255)                   ttttCACGGAGTTGCTTAATAATGTCCCGCACCGGAGAGGCAAACGCCGCCTGCAGGTTATTCCTTTTCTCGCCCGGTACGTCGAGGGTG‑ATAACGGCAATGTTGTCCAGACGAACATTAAGGGTAAACGCTGATGTCATTCCCATTATTCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGTTAACTAAACCAAAT‑CCACCGCCGCGACGGCGAAGTTCATGc                                                                                                                                                                                                                    <  2:1052264/239‑1 (MQ=255)                        aCGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGTACGTCGATGGTG‑ATAACGGCAATGTTGTCCAGACGAACATTAAGGGTAAACGCTGATGTCATTTCCATTATTCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGTAACTAAACCAAAT‑CCACCGCCGCGACGGCGAAGTTCCTGCAATg                                                                                                                                                                                                                >  1:323288/1‑238 (MQ=255)                           gAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGTACGTCGATGGTG‑ATAACGGCAATGTTGTCCAGACGAACATTAAGGGTAAACGCTGATGTCATTTCCATTATTCCGCCTCCAGAACCATTGCCGAGCCAAGCCCACCGGCAGCACAGGCGGTAACTAAACCAAAT‑CCACCGCCGCGACGGCGAACTCCATGCAATGTATg                                                                                                                                                                                                            >  2:1138794/1‑239 (MQ=255)                             gTTGCTTAATAATGGCGCGCGCCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGTACGTCGATGGTG‑ATAACGGCAATGTTGTCCAGACGAACATTAAGGGTAAACGCTGATGTCATTTCCATTATTCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGTCACTAAACCAAAC‑CCACCCCCCCCACGCCGAAGTTCATGCAATGGCgggg                                                                                                                                                                                                          >  2:678271/1‑239 (MQ=255)                                      taatGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGTACGTCGATGGTG‑ATAACGGCAATGTTGTCCAGACGAACATTAAGGGTAAACGCTGATGTCATTTCCATTATTCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGTAACTAAACCAAAT‑CCACCGCCGCGACGGCGAAGTTCATGCAATGTCTGGGTAatcatc                                                                                                                                                                                                  >  1:804327/1‑238 (MQ=255)                                      taatGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGTACGTCGATGGTG‑ATAACGGCAATGTTGTCCAGACGAACATTAAGGGTAAACGCTGATGTCATTTCCATTATTCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGTAAATAAACCCAAT‑CCACCCCCGCGACGGCCCAGGTCATGCAACTTCCGGGTAatcatc                                                                                                                                                                                                  >  2:1217488/1‑238 (MQ=255)                                        atGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGTACGTCGATGGTG‑ATAACGGCAATGTTGTCCAGACGAACATTAAGGGTAAACGCTGATGTCATTTCCATTATTCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGTAACTAAACCAAAT‑CCACCGCCGCGACGGCGAAGTTCATGCAATGTCTGGGAAATCATCcgc                                                                                                                                                                                               >  1:731851/1‑239 (MQ=255)                                        atGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGTACGTCGATGGTG‑ATAACGGCAATGTTGTCCAGACGAACATTAAGGGTAAACGCTGATGTCATTTCCATTATTCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGTAACTAAACCAAAT‑CCACCGCCGCCACGGCGAAGTTCATGCACTGTCTGGGTAATCATCcg                                                                                                                                                                                                >  2:719790/1‑238 (MQ=255)                                           gcgcgcACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGTACGTCGATGGTG‑ATAACGGCAATGTTGTCCAGACGAACATTAAGGGTAAACGCTGATGTCATTTCCATTATTCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACCGGCGGTAACTAAACCAAAT‑CCCCCGCCGCCACCGCGAACACCATGCCATGTCCGGGCAATCATCcgccc                                                                                                                                                                                             >  2:607416/1‑236 (MQ=255)                                           gcgcgcACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGTACGTCGATGGTG‑ATAACGGCAATGTTGTCCAGACGAACATTAAGGGTAAACGCTGATGTCATTTCCATTATTCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGTAACTAAACCAAAT‑CCACCGCCGCGCCGGCGAAGTTCATGCAATGTCTGGGTAATCATcccccc                                                                                                                                                                                             >  2:916620/1‑234 (MQ=255)                                            cgcgcACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGTACGTCGATGGTG‑ATAACGGCAATGTTGTCCAGACGAACATTAAGGGTAAACGCTGATGTCATTTCCATTATTCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGTAACTAAACCAAAT‑CCACCGCCGCGACGGCGAAGTTCATGCAATGTCTGGGTAATCATCcgcccg                                                                                                                                                                                            >  1:497010/1‑238 (MQ=255)                                              cgcACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCCCCCGGTACGTCGATGGTG‑ATAACGGCAATGTTGTCCAGACGAACATTAAGGGTAAACGCTGATGTCATTTCCATTATTCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGTAACTAAACCAAAT‑CCACCGCCGCGACGGCGAAGTTCATGCAATGTCt                                                                                                                                                                                                             <  1:1088432/219‑1 (MQ=255)                                              cgcACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGTACGTCGATGGTG‑ATAACGGCAATGTTGTCCAGACGAACATTAAGGGTAAACGCTGATGTCATTTCCATTATTCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGTAACTAAACCAAAT‑CCACCGCCGCGCCGCCGAAGGTCATGCCACGACTGGGTAAGCATCCGCGCCCCg                                                                                                                                                                                         >  2:142946/1‑239 (MQ=255)                                              cgcACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGTACGTCGATGGTG‑ATAACGGCAATGTTGTCCAGACGAACATTAAGGGTAAACGCTGATGTCATTTCCATTATTCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGTAACTAAACCAAAT‑CCACCGCCGCGACGGCGAAGTTCATGCAATGTCTGGGTAATCATCCGCGCGCCg                                                                                                                                                                                         >  1:1070744/1‑239 (MQ=255)                                              cgcACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGTACGTCGATGGTG‑ATAACGGCAATGTTGTCCAGACGAACATTAAGGGTAAACGCTGATGTCATTTCCATTATTCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGTAACTAAACCAAAT‑CCACCGCCGCCACGGAGAAGTTCATGCAATGTCTGGGTAATCATCCGCGCGCCg                                                                                                                                                                                         >  1:1112370/1‑239 (MQ=255)                                              cgcACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTATCACCCGGAACGTCGATGGTG‑ATAACGACAATGTTGTCCAGACGAACATTAAGGGTAAACGCTGATGTCATTTCCATTATTACGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCATCACAGGCGGTAACTAAACAAAAT‑CCACCACCGCGACCGCGAAGTTCATGCAATGTCTGGGTAATACACCGCGCGCCg                                                                                                                                                                                         >  2:270176/1‑239 (MQ=255)                                                cACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGTACGTCGATGGTG‑ATAACGGCAATGTTGTCCAGACGAACATTAAGGGTAAACGCTGATGTCATTTCCATTATTCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGTAACTAAACCAAAT‑CCACCGCCGCGACGGCGAAGTTCATGCAATGTCTGGGTAATCATCCGCGCGCCgg                                                                                                                                                                                        >  1:806809/1‑238 (MQ=255)                                                       gAGGCAAACTCCGCCTTCAGGTTATTCATTTTCTCACCCGGTACGTCGATGGTG‑ATAACGGCAATGTTGTCCAGACGAACATAAAGGGTAAACGCTGATGTCATTTCCATTATTCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGTAACTAAACCAAAT‑CCACCGCCGCGACGGCGAAGTTCATGCAATGTCTGGGTAATCATCCGCGCGCCGGTcgccgc                                                                                                                                                                                 <  1:201403/238‑1 (MQ=255)                                                            aaagttcGCCTTCAGGTTATTCATTTTCTCCCCCGTACCGTCAATGGTA‑ACAACGGCAATCTTCGCCAGACGAACATTAGGGTTAAACGCTGATGCCATTCCCATTATTCCGCCTCCAGAACCATTACCGCGCCAAGCCCACCGGCAGCAAAGGCGGTAACTAACCCAAAC‑CCACCGCCGCGACGGCGAAGTTCATGCAATGTCTGGGTAATCATCCGCGCGCCGGTCGCCGCGAAgg                                                                                                                                                                            <  2:566043/232‑1 (MQ=255)                                                             actgccGCCTTCAGGGTACTCCTTTCCCCCCACGGTACGTCGATGGTG‑ATAACGGCAATGTTTTCCCGCCAAACATTAGGGGTAAACGCGGAGTGCATCTCCATTATCCTGCCTCCAGAACCATTGCCGCCCCAAGCCCACGGCCAGCACAGGCGTTAACAAAACCAAAT‑CCCCCGCCGCGACGGCGAAGTTCTTGCAATGTCTGGGTAATCATCCGCGCGCCGGTCGCCGCGAAGGGa                                                                                                                                                                          <  2:575149/235‑1 (MQ=255)                                                             aaCTCCGCCTTCAGGGTATTCATTTTCTCACCCGGTCCGTCGATGGTG‑ATAACGGCAATGTGGTCCAGACGAACATTAAGGGTAAACGCTGATGTCATTTCCATTATTCCGCCTCCAGAACCATTGCCGCGCCAAGCCCCCCGGCAGCACAGGCGGTAACTAAACCAAAT‑CCACCGCCGCGACGGCGAAGTTCATGCAATGTCTGGGTAATCATCCGCGCGCCGGTCGCCGCGAAGGGa                                                                                                                                                                          <  1:125365/239‑1 (MQ=255)                                                             aaCTCCGCCTTCAGGGTATTCATTTTCTCACCCGGTACGTCGATGGTG‑ATAACGGCAATGTTGTCCAGACGAACATTAAGGGTAAACGCTGATGTCATTTCCATTATTCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGTAACTAAACCAAAT‑CCACCGCCGCGACGGCGAAGTTCATGCAATGTCTGGGTAATCATCCGCGCGCCGGTCGCCGCGAAGGGa                                                                                                                                                                          >  1:951475/1‑239 (MQ=255)                                                             aaCGCCGCCTTAAGAGTATTCTTTTACGCCCCCGGTGCGTCGAGGGTG‑ATAACGGCACTGTTGTCCAGCCGACCATTAAGGTAAACCCCGGAGGTCACCCCCATTACCCCGCCTAAAGAACCATGGCGGCGCCAAGCCCACGGGCAGAAGAGGCGTAAACTACGCAAAAT‑CCCCCGCCGCGACGGCGAAGTTCATGCAATGTCTGGGTAATCATCCGCGCGCCGGTCGCCGCGAAGGGa                                                                                                                                                                          <  2:1070744/239‑1 (MQ=255)                                                                               ttCATTTTCTCACCCGGTACGTCGATGGTG‑ATAACGGCAATGTTGTCCAGACGAACATTAAGGGTAAACGCTGATGTCATTTCCATTATTCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGTGACTAAACCAAAT‑CCACCCCCGCGCCGGCGAAGGTCATCCCATGTCTGGGTAATCATCCGCGCGCCGGTCGCCGCGACGGGGTGCCCGTAAGCAATCgg                                                                                                                                                         >  2:1007058/1‑237 (MQ=255)                                                                                     ttcccccccGGTACGTCGATGGTG‑ATAACGGCAATGTTGTCCAGACGAACATTAAGGGTAAACGCTGATGTCATTCCCATTATCCCGCCCCCAGAACCATTGCGGCGCCAAGCCCACCGGCAGCACAGGCGGTAACTAAACAAAAT‑CCACCGCCGCGACGGCGAAGTTCATGCAATGTCTGGGTAATCATCCGCGCGCCGGGCGCCGCGAAGGGATGCCCGTAAGCAATCGAACCGCCa                                                                                                                                                  <  1:695692/233‑1 (MQ=255)                                                                                     ttCTCACCCGGTACGTCGATGGTG‑ATAACGGCAATGTTGTCCAGACGAACATTAAGGGTAAACGCTGATGTCATTTCCATTATTCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGTAACTAAACCAAAT‑CCACCGCCGCGACGGCGAAGTTCATGCAATGTCTGGGTAATCATCCGCGCGCCGGTCGCCGCGAAGGGATGCCCGTAAGCAATCGAACCGCCa                                                                                                                                                  >  1:36805/1‑239 (MQ=255)                                                                                     ttCTCACCCGGTACGTCGATGGTG‑ATAACGGCAATGTTGTCCAGACGAACATTAAGGGTAAACGCTGATGTCATTTCCATTATTCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGTAACTAAACCAAAT‑CCACCGCCGCGACGGCGAAGTTCATGCAATGTCTGGGTAATCATCCGCCCGCCGGTCGCCGCGAAGGGATGCCCGTAAGCAATCGAACCGccc                                                                                                                                                  >  1:860818/1‑238 (MQ=255)                                                                                     ggcccccccGGTACG‑CGAAGGGGTATACCGGAAATGTTGCCCCCGCGAAAATTAAGGGCAAACCCTTATGTCTTTCCCATTCCCCCGCCCCCAGACCCATCGCCGCCCCAAGCCCCCCGGCAGCACAGGCGGTAACTAACCCAAAC‑CCCCCGCCGCGACGGCGAAGTTCATGCAATGTCTGGGTAATCACCCGCGCGCCGGTCGCCGCGAAGGGATGCCCGTAAGCAATCGAACCGCCa                                                                                                                                                  <  2:690205/233‑1 (MQ=255)                                                                                       ctcACCCGGTACGTCGATGGTG‑ATAACGGCAATGTTGTCCAGACGAACATTAAGGGTAAACGCTGATGTCATTTCCATTATTCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGTAACTAAACCAAAT‑CCACCGCCGCGACGGCGAAGTTCATGCAATGTCTGGGTAATCATCCGCGCGCCGGTCGCCGCGAAGGGATGCCCGTAAGCAATCGAACCGCCaa                                                                                                                                                 >  1:988657/1‑238 (MQ=255)                                                                                       ctcACCCGGTACGTCGATGGTG‑ATAACGGCAATGTTGTCCAGACGAACATTAAGGGTAAACGCTGATGTCATTTCCATTATTCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGTAACTAAACCAAAT‑CCACCGCCGCGACGGCGAAGTTCATGCAATGTCTGGGTAATCATCCGCGCGCCGGTCGCCGCGAAGGGATGCCCGTAAGCAATCGAACCGCCAAg                                                                                                                                                <  1:111588/239‑1 (MQ=255)                                                                                         cACCCGGTACGTCGATGGTG‑ATAACGGCAATGTTGTCCAGACGAACATTAAGGGTAAACGCTGATGTCATTTCCATTATTCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGTAACTAAACCAAAT‑CCACCGCCGCGACGGCGAAGTTCATGCAATGTCTGGGTAATCATCCGCGCGCCGGTCGCCGCCAAGGGATGCCCGTAAGCAATCGAACCGCCAAgg                                                                                                                                               >  1:584007/1‑237 (MQ=255)                                                                                            cggttgcggcGATGGTT‑ATAACAGCATTGTTTGCCAGACGAACATTAAGGGTAAGCGCTGGCGCCACCTCCATTATCCCGCCTCCAAAACCATGGCCCCCCGAACCCCCCCGGCACAACAGGCGGAAACTAACCAAAAC‑CCCCCGCCGCGACGGCGAAGTTCATGCAATGTCTGGGTAATCAGCCGGGCGCCGGTCGCCGCGAAGGGATGCCCGTAAGCAATCGAACCGCCAAGCACGt                                                                                                                                           <  2:34581/230‑1 (MQ=255)                                                                                              ggTACGTCGATGGTG‑ATAACGGCAATGTTGTCCAGACGAACATTAAGGGTAAACGCTGATGTCATTTCCATTATTCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGTAACTAAACCAAAT‑CCACCGCCCCGACGGCGAAGTTCATGCCATGTCTGGGTAATCCTCCGCGCCCCGGTCCCCCCGAAGGGATGCCCCTAAGCAATCGACCCCCCACCCCCGTTa                                                                                                                                         >  2:451383/1‑239 (MQ=255)                                                                                                      gATGGTG‑ATAACGGCAATGTTGTCCAGACGAACATTAAGGGTAAACGCTGATGTCATTTCCATTATTCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGTAACTAAACCAAAT‑CCACCGCCGCGACGGCGAAGTTCATGCAATGTCTGGGTAATCATCCGCGCGCCGGTCCCCGCGAAGGGATGCCCGTAAGCAATCGGACCGCCAAGCACGTTAAATTTGCt                                                                                                                                 >  1:710583/1‑239 (MQ=255)                                                                                                          gTG‑ATAACGGCAATGTTGTCCAGACGAACATTAAGGGTAAACGCTGATGTCATTTCCATTATTCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGTAACTAAACCAAAT‑CCACCGCCGCGACCGCGAAGTTCATGCAATGTCTGGGTAATCATCCGCGCGCCGGTCGCCGCGAAGGGATGCCCGTAAGCAATCGAACCGCCAAGCACGTTAAATTTGCTATCg                                                                                                                             >  1:645245/1‑239 (MQ=255)                                                                                                                accGGCAATGTTGTCCAAACGAACATTAAGGGTAAACGCTGATGTCATTCCCATTATTCCGCCTCCAGAACCATTCCCCCGCCAAGCCCCCCGGCAGCACAGGCGGTAACTAAACCAAAC‑CCACCGCCGCGACGGCGAAGTTCATGCAATGTCTGGGTAATCATCCGCGCGCCGGTCGCCGCGAAGGGATGCCCGTAAGCAATCGAACCGCCAAGCACGTTAAATTTGCTATCGTCCAc                                                                                                                        <  1:451383/237‑1 (MQ=255)                                                                                                                aaCGGCAATGTTGTCCAGACGAACATTAAGGGTAAACGCTGATGTCATTTCCATTATTCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGTAACTAAACCAAAT‑CCACCGCCGCGCCGGCGAAGTTCATGCAATGTCTGGGTAATCATCCGCGCGCCGGCCCCCGCGAAGGGATGCCCGTAAGCAATCGAACCGCCAAGCACGTTCACTTTGCTATCGGcc                                                                                                                          >  1:626486/1‑237 (MQ=255)                                                                                                                     cAATGTTGTCCAGACGAACATTAAGGGTAAACGCTGATGTCATTTCCATTATTCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGTAACTAAACCAAAT‑CCACCGCCGCGACGGCGAAGTTCATGCAATGTCTGGGTAATCATCCGCGCGCCGGTCGCCGCGAAGGGATGCCCGTAAGCAATCGAACCGCCAAGCACGTTAAATTTGCTATCGTCCACTTCg                                                                                                                    >  1:241922/1‑238 (MQ=255)                                                                                                                        tgttgtCCAGACGAACATTAAGGGTAAACGCTGATGTCATTTCCATTAGTCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGTAACTAAACCAAAT‑CCACCGCCGCGACGGCGAAGTTCATGCAATGTCTGGGTAATCATCCGCGCGCCGGTCGCCGCGAAGGGATGCCCGTAAGCAATCGAACCGCCAAGCACGTTAAATTTGCTATCGTCCACTTCGCCAg                                                                                                                <  1:1054794/239‑1 (MQ=255)                                                                                                                            gtCCAGACGAACATTAAGGGTAAACGCTGATGTCATTCCCATTATTCCGCCTCCAGAACCATTGCCGCGCCAAGCCCCCCGGCAGCACAGGCGGTAACTAAACCAAAT‑CCACCGCCGCGACGGCGAAGTTCATGCAATGTCTGGGTAATCATCCGCGCGCCGGTCGCCGCGAAGGGATGCCCGTAAGCAATCGAACCGCCAAGCACGTTAAATTTGCTATCGTCCACTTCGCCAGTGGc                                                                                                            <  1:895745/239‑1 (MQ=255)                                                                                                                             tCCAGACGAACATTAAGGGTAAGCGCGGAGGGCGTTCCCATTTCCCCGCACCCAGAACAATTCCCGCGCCACGCCCCCGGGCAGCACGGGCGGTAACTAAACCAAAC‑CCACCGCCGCGACGGCGAAGTTCATGCAATGTCTGGGTAATCATCCGCGCGCCGGTCGCCGCGAAGGGATGCCCGTAAGCAATCGAACCGCCAAGCACGTTAAATTTGCTATCGTCCACTTCGCCAGTGGc                                                                                                            <  2:268301/238‑1 (MQ=255)                                                                                                                             tCCAGACGAACATTAAGGGTAAACGCTGATGTCATTTCCATTATTCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGTAACTAAACCAAAT‑CCACCGCCGCGACGGCGAAGTTCATGCAATGTCTGGGTAATCATCCGCGCGCCGGTCGCCGCGAAGGGATGCCCGTAAGCAATCGAACCGCCCAGCACGTTAAATTTGCTATCGTCCACTTCGCCAGGGGcg                                                                                                           >  2:212964/1‑238 (MQ=255)                                                                                                                             tCCAGACGAACATTAAGGGTAAACGCTGATGTCATTTCCATTATTCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGTAACTAAACCAAAT‑CCACCGCCGCGACGGCGAAGTTCATGCAATGTCTGGGTAATCATCCGCGCGCCGGTCGCCGCGAAGGGATGCCCGTAAGCAATCGAACCGCCAAGCACGTTAAATTTGCTATCGTCCACTTCGCCAGTGGCa                                                                                                           >  1:244298/1‑239 (MQ=255)                                                                                                                             cccagccaaccATTAAGGTAAAACGCTGATCCCATTCCCATTATCCCGCCCCCAAACCCACTGCCGCGCCACCCCCACGGGCAGCACAGGCGTTAACTAACCAAAAC‑CCCCCGCCGCGACGGCGAAGTTCATGCAATGTCTGGGTAATCATCCGCGCGCCGGTCGCCGCGAAGGGATGCCCGTAAGCAATCGAACCGCCAAGCACGTTAAATTTGCTATCGTCCACTTCGCCAGTGGCa                                                                                                           <  2:584007/229‑1 (MQ=255)                                                                                                                              ccAGACGAACATTAAGGGTAAACGCTGATGTCATTTCCATTATTCCGCCTCCAGACCCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGTAACTAAACCAAAC‑CCACCGCCGCGACGGCGAAGTTCATGCAATGTCTGGGTAATCATCCGCGCGCCGGTCGCCGCGAAGGGATGCCCGTAAGCAATCGAACCGCCAAGCACGTTAAATTTGCTATCGTCCACTTCGCCAGTGGCAt                                                                                                          <  1:678271/239‑1 (MQ=255)                                                                                                                               cAGACGAACATTAAGGGTAAACGCTGATGTCATTTCCATTATTCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGTAACTAAACCAAAT‑CCACCGCCGCGACGGCGAAGTTCATGCAATGTCTGGGTAATCATCCGCGCGCCGGTCGCCGCGAAGGGATGCCCGAAAGCAATCGAACCGCCAAGCACGTTAAATTTGCTATCGTCCACTTCGCCAGTGGCAtg                                                                                                         >  1:169061/1‑239 (MQ=255)                                                                                                                                   gcaaaagtaggggTAAACGCTGAGGTCATTTCCATACGCCCGCCTCCAGAACCATCGCCGCGCCAACCCCACCGGAAGCACGGGCGGAAACAAAACCAAAC‑CCCCCGCCGCGACGGCGAAGGTCATGCAATGTCTGGGTAATCATCCGCGCGCCGGTCGCCGCGAAGGGATGCCCGTAAGCAATCGAACCGCCAAGCACGTTAAATTTGCTATCGTCCACTTCGCCAGTGGCATGTGCa                                                                                                     <  2:912746/229‑1 (MQ=255)                                                                                                                                   caacaaTTAGGGGAAAACCCGGATGTCATTTCCCTTATCCCGCCTCCAGACCCATTGCCGCCCCAACCCCACCGGCAGCCCAGGCGGTACCTAACCCAAAT‑CCACCGCCGCGACGGCGAAGTTCATGCAATGTCTGGGTAATCATCCGCGCGCCGGTCGCCGCGAAGGGATGCCCGTAAGCAATCGAACCGCCAAGCACGTTAAATTTGCTATCGTCCACTTCGCCAGTGGCATGTGCa                                                                                                     <  2:755462/234‑1 (MQ=255)                                                                                                                                     caCATTAGTGATAAACGCTGATGTCTTTTCTATTAATCGGCACCAAGAACCACACCCGCGCCAAGCCCCCCGGCAGCTCAGGCGGTATCTAACCCAAAT‑CCCCCGCCGCGACGGCGAAGTTCATGCAATTTCTGGGTAATCATCCGCGCGCCGGTCGCCGCGAAGGGATGCCCGTAAGCAATCGAACCGCCAAGCACGTTAAATTTGCTATCGTCCACTTCGCCAGTGGCATGTGCACg                                                                                                   <  2:908739/238‑1 (MQ=255)                                                                                                                                       cATTAAGGGTAAACGCTGATGTCATTTCCATTATTCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGTAACTAAACCAAAT‑CCACCGCCGCGACGGCGAAGTTCATGCAATGTCTGGGTAATCATCCGCGCGCCGGTCGCCGCGAAGGGATGCCCGTAAGCAATCGAACCGCCAAGCACGTTAAATTTGCTATCGTCCACTTCGCCAGTGGCATGTGCACGc                                                                                                  >  1:511207/1‑238 (MQ=255)                                                                                                                                                  aaCGCTGATGTCATTTCCATTATTCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGTAACTAAACCAAAT‑CCACCGCCGCGACGGCGAAGTTCATGCAATGTCTGGGTAATCATCCGCGCGCCGGTCGCCGCGAAGGGATGCCCGTAAGCAATCGAACCGCCAAGCACGTTAAATTTGCTATCGTCCACTTCGCCAGTGGCATGTGCCCGCCCCAGTGCTTc                                                                                       >  1:677782/1‑238 (MQ=255)                                                                                                                                                  aaCGCTGATGTCATTTCCATTATTCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGTAACTAAACCAAAT‑CCACCGCCGCGACGGCGAAGTTCATGCAATGTCTGGGTAATCATCCGCGCGCCGGTCGCCGCGAAGGGATGCCCGTAAGCAATCGAAACGCCAAGCACGTTAAATTTGCTATCGTCCACTTCGCCAGTGGCATGTGCAcccccccaggcttc                                                                                       >  1:13280/1‑230 (MQ=255)                                                                                                                                                        gATGTCATTTCCATTATTCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGTAACTAAACCAAAT‑CCACCGCCGCGACGGCGAAGTTCATGCAATGTCTGGGTAATCATCCGCGCGCCGGTCGCCGCGAAGGGATGCCCGTAAGCAATCGAACCGCCAAGCACGTTAACTTTGCTATCGTCCACTTCGCCAGTGGCATGTGCCCCCCCCAGGTCTTCaaggaca                                                                                >  1:478295/1‑233 (MQ=255)                                                                                                                                                        gATGTCATTTCCATTATTCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGTAACTAAACCAAAT‑CCACCGCCGCGACGGCGAAGTTCATGCAATGTCTGGGAAATAATCCCCGCCCCGGTCGCCGCGAAGGGATGCCCGTAAGCAATCGAACCGCCCAGCCCGGTCAATTTGCCATCGTCCACTTCGCCAGAGGCATGAGCACCCCCCAGTGGTTCAccggtc                                                                                >  2:427373/1‑234 (MQ=255)                                                                                                                                                          tttCATTCCCATTATCCCGCCTCCAGAACCATGCCCGCGCCAAGCCCACCGGCAGCACAGGCGGTAACTAAACCAAAT‑CCGCCGCCGCGACGGCGAAGTTCATGCAATGTCTGGGTAATCATCCGCGCGCCGGTCGCCGCGAAGGGATGCCCGTAAGCAATCGAACCGCCAAGCACGTTAAATTTGCTATCGTCCACTTCGCCAGTGGCATGTGCACGCCCCAGTGCTTCACGAGCaaa                                                                              <  1:962885/237‑1 (MQ=255)                                                                                                                                                             aaTTTCCATTATTCCGCCTCCAGAACCATGGCCGCGCCAAGCCCACCGGCAGCACAGGCGGTAACTAAACCAAAT‑CCCCCGCCGCGACGGCGAAGTTCATGCAATGTCTGGGTAATCATCCGCGCGCCGGTCGCCGCGAAGGGATGCCCGTAAGCAATCGAACCGCCAAGCACGTTAAATTTGCTATCGTCCACTTCGCCAGTGGCATGTGCACGCCCCAGTGCTTCACGAGCAAAAc                                                                            <  1:328004/237‑1 (MQ=255)                                                                                                                                                                 tcaattatcCCGCCACAAAAACCATACCCGCCCCAAGCCCCCCGACACCACGGGGGGAACCAAAGCAAAAC‑CCCCCGCCGCGACGGCGAAGTTCATGCAATGTCTGGGTAATCATCCGCGCGCCGGTCGCCGCGAAGGGATGCCCGTAAGCAATCGAACCGCCAAGCACGTTAAATTTGCTATCGTCCACTTCGCCAGTGGCATGTGCACGCCCCAGTGCTTCACGAGCAAAACGTTCa                                                                       <  2:511207/236‑1 (MQ=255)                                                                                                                                                                 cccAATATCCCGCCCCCAGAACCATCGCCGCCCCACCCCCCCGGGCACCACAGGCGTAAACAAAACCAAAC‑CCCCCGCCGCGACGGCGAAGTTCATGCAATGTCTGGGTAATCACCCGCGCGCCGTTCGCCGCGAAGGGATGCCCGTAAGCAATCGAACCGCCAAGCACGTTAAATTTGCTATCGTCCACTTCGCCAGTGGCATGTGCACGCCCCAGTGCTTCACGAGCAAAACGtt                                                                         <  2:497010/236‑1 (MQ=255)                                                                                                                                                                  ccATAAGTCCGCCTCCAAAACCACGCCCGCGCCAGCCCCCCGGGCGGCACGGCCGGAAACAAACCAAAAC‑CCCCCGCCGCGACGGCGAAGTTCATGCAATGTCTGGGTAATCATCCGCGCGCCGGTCGCGGCGAAGGGATGCCCGTAAGCAATCGAACCGCCAAGCACGTTAAATTTGCTATCGTCCACTTCGCCAGTGGCATGTGCACGCCCCAGTGCTTCACGAGCAAAACGTTCAc                                                                      <  2:626486/239‑1 (MQ=255)                                                                                                                                                                      tattCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGTAACTAAACCAAAT‑CCACCGCCGCGACGGCGAAGTTCATGCAATGTCTGGGTAATCATCCGCGCGCCGGTCGCCGCGAAGGGATGCCCGTAAGCAATCGAACCGCCAAGCACGTTAAATTTGCTATCGTCCACTTCCCCAGTGGCATGTGCACGCCCCAGTGCTTCACGAGCAAAAAGTTCACTAc                                                                   >  1:1110662/1‑238 (MQ=255)                                                                                                                                                                           cGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGTAACTAAACCAAAT‑CCACCGCCGCGACGGCGAAGTTCATGCAATGTCTGGGTAATCATCCGCGCGCCGGTCGCCGCGAAGGGATGCCCGTAAGCAATCGAACCGCCAAGCACGTTAAATTTGCTATCGTCCACTTCGCCAGTGGCATGTGCACGCCCCAGTGCTTCACGAGCAAAACGTTCACTACccacca                                                             >  1:457098/1‑239 (MQ=255)                                                                                                                                                                            gCCCCCAAAACCACTCCCGGCCGAACCCCGCTGGCAGGTCCGGGGGGAACTAACACAAAC‑CCCCCGCCGCGACGGCGAATTTCATGCAAGGTCTGGGAAATCATCCGCGCGCCGGTCGCCGCGAAGGGATGCCCGTAAGCAATCGAACCGCCAAGCACGTTAAATTTGCTATCGTCCACTTCGCCAGTGGCATGTGCACGCCCCAGTGCTTCACGAGCAAAACGTTCACTACCCAGCaa                                                            <  2:957921/239‑1 (MQ=255)                                                                                                                                                                             ccTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGTAACTAAACCAAAT‑CCACCGCCGCGACGGCGAAGTTCATGCAATGTCTGGGTAATCATCCGCGCGCCGGTCGCCGCGAAGGGATGCCCGTAAGCAATCGAACCGCCAAGCACGTTAAATTTGCTATCGTCCACTTCGCCCGTGGCATGTGCACGCCCCAGTGCTTCACGAGCAAAAAGTTCCACACCCAGCaaa                                                           >  1:952292/1‑238 (MQ=255)                                                                                                                                                                                         ttGCCGCGCCAAGCCCACCGGCAGCACAGGCGGTAACTAAACCAAAT‑CCACCGCCGCGACGGCGAAGTTCATGCAATGTCTGGGTAATCATCCGCGCGCCGGTCGCCGCGAAGGGATGCCCGTAAGCAATCGAACCGCCAAGCACGTTAAATTTGCTATCGTCCACTTCGCCAGTGGCATGTGCACG‑CCCAGTGCTTCCCGAGCAAAACGTTCACTACCCAGCAACTGAATATTCGCCa                                              >  1:145421/1‑239 (MQ=255)                                                                                                                                                                                         tggCCGCGCCAAGCCCGCCGGCAGCACAGGCGGTAACTAAACCAAAT‑CCCCCGCCGCGACGGCGAAGTTCATGCAATGTCTGGGTAATCATCCGCGCGCCGGTCGCCGCGAAGGGATGCCCGTAAGCAATCGAACCGCCAAGCACGTTAAATTTGCTATCGTCCACTTCGCCAGTGGCATGTGCACGCCCCAGTGCTTCACGAGCAAAACGTTCACTACCCAGCAACTGAATATTCGcc                                               <  1:1217488/237‑1 (MQ=255)                                                                                                                                                                                           gCCGCGCCAAGCCCACCGGCAGCACAGGCGGTAACTAAACCAAAT‑CCACCGCCGCGACGGCGAAGTTCATGCAATGTCTGGGTAATCATCCGCGCGCCGGTCGCCGCGAAGGGATGCCCGTAAGCAATCGAACCGCCAAGCACGTTAAATTTGCTATCGTCCACTTCGCCAGTGGCATGTGCACGCCCCAGTGCTTTACGAGCAAAACGTTCACTACCCAGCAACTGCATATTCGCCa                                              >  1:708362/1‑238 (MQ=255)                                                                                                                                                                                                  cacgCCCCCCGGAAGCACAGGCGGTAACTAAACAAAAC‑CCCCCGCCGCGACGGCGAAGTTCATGCAATGTCTGGGTAATCATCCGCGCGCCGGTCGCCGCGAAGGGATGCCCGTAAGCAATCGAACCGCCAAGCACGTTAAATTTGCTATCGTCCACTTCGCCAGTGGCATGTGCACGCCCCAGTGCTTCACGAGCAAAACGTTCACTACCCAGCAACTGAATATTCGCCAGCGTCTGa                                      <  2:478295/236‑1 (MQ=255)                                                                                                                                                                                                        cACCGCCAGCACAGGCGGTAACTAACCCAACT‑CCCCCGCCGCGACGGCGAAATTCATGCAATGTCTGGGTAATCATCCGCGCGCCGGTCGCCGCGAAGGGATGCCCGTAAGCAATCGAACCGCCAAGCACGTTAAATTTGCTATCGTCCACTTCGCCAGTGGCATGTGCACGCCCCAGTGCTTCACGAGCAAAACGTTCACTACCCAGCAACTGAATATTCGCCAGCGTCTGAGCTGCa                                <  1:607416/239‑1 (MQ=255)                                                                                                                                                                                                         acgggCAGCACAGGCGTTAACAAAACCAAAC‑CCACCGCCGCGACGGCGAAGTTCATGCAATGTCTGGGTAATCATCCGCGCGCCGGTCGCCGCGAAGGGATGCCCGTAAGCAATCGAACCGCCAAGCACGTTAAATTTGCTATCGTCCACTTCGCCAGTGGCATGTGCACGCCCCAGTGCTTCACGAGCAAAACGTTCACTACCCAGCAACTGAATATTCGCCAGCGTCTGAGCTGCaa                               <  1:1007058/236‑1 (MQ=255)                                                                                                                                                                                                              ggccccaGGCGGTAACAAAACCAACC‑CCGCCGCCGCGACGGGGAAGTTCATGCAATGTCTGGGTAATCACCCGCGCGCCGGTCGCCGCGAAGGGATGCCCGTAAGCAATCGAACCGCCAAGCCCGTTAAATTTGCTATCGTCCACTTCGCCAGTGGCATGTGCACGCCCCAGTGCTTCACGAGCAAAACGTTCACTACCCAGCAACTGAATATTCGCCAGCGTCTGAGCTGCAAAGGc                           <  2:645245/233‑1 (MQ=255)                                                                                                                                                                                                               agcaCAGGCGGTAACTAACCCAAAC‑CCACCGCCGCGACGGCGAAGTTCATGCAATGTCTGGGTAATCATCCGCGCGCCGGTCGCCGCGAAGGGATGCCCGTAAGCAATCGAACCGCCAAGCACGTTAAATTTGCTATCGTCCACTTCGCCAGTGGCATGTGCACGCCCCAGTGCTTCACGAGCAAAACGTTCACTACCCAGCAACTGAATATTCGCCAGCGTCTGAGCTGCAAAGGCt                          <  1:808475/238‑1 (MQ=255)                                                                                                                                                                                                                       ggtaaaCTAAACCAAAC‑CCCCCGCCGCGACGGCGAAGTTCATGCAATGTCGGGGTAATCATCCGCGCGCCGGTCGCCGCGAAGGGATGCCCGTAAGCAATCGAACCGCCAAGCACGTTAAATTTGCTATCGTCCACTTCGCCAGTGGCATGTGCACGCCCCAGTGCTTCACGAGCAAAACGTTCACTACCCAGCAACTGAATATTCGCCAGCGTCTGAGCTGCAAAGGCTTCGTGCat                  <  1:240151/234‑1 (MQ=255)                                                                                                                                                                                                                        gttAACAAACCAAAAC‑CCCCCGCCGCGACGGCGAAGTTCATGCAATGTCTGGGTAATCATCCGCGCGCCGGTCGCCGCGAAGGGATGCCCGTAAGCAATCGAACCGCCAAGCACGTTAAATTTGCTATCGTCCACTTCGCCAGTGGCATGTGCACGCCCCAGTGCTTCACGAGCAAAACGTTCACTACCCAGCAACTGAATATTCGCCAGCGTCTGAGCTGCAAAGGCTTCGTGCata                 <  1:498283/236‑1 (MQ=255)                                                                                                                                                                                                                        ggTACCTAAACAAAAT‑CCCCCCCCGCGACGGCGAAGTTCATGCAATGTCTGGGTAATCAGCCGCGCGCCGGTCGCCGCGAAGGGATGCCCGTAAGCAATCGAACCGCCAAGCACGTTAAATTTGCTATCGTCCACTTCGCCAGTGGCATGTGCACGCCCCAGTGCTTCACGAGCAAAACGTTCACTACCCAGCAACTGAATATTCGCCAGCGTCTGAGCTGCAAAGGCTTCGTGCata                 <  1:270176/238‑1 (MQ=255)                                                                                                                                                                                                                            acaaacccAAAC‑CCCCCGCCGCGACGGCGAAGGTCATGCAATGTCTGGGTAATCATCCGCGCGCCGGTCGCCGCGAAGGGATGCCCGTAAGCAATCGAACCGCCAAGCACGTTAAATTTGCTATCGTCCACTTCGCCAGTGGCATGTGCACGCCCCAGTGCTTCACGAGCAAAACGTTCACTACCCAGCAACTGAATATTCGCCAGCGTCTGAGCTGCAAAGGCTTCGTGCATATCGAt            <  1:222981/233‑1 (MQ=255)                                                                                                                                                                                                                            acaaaaCCAACT‑CCGCCGCCGCGGCTGCAGAGTTCATGCAAGGCTGGGGTAATCACCCGCGCGGCGGTCGCCGCGAAGGGATGCCCGTAAGCAATCGAGCCGCCAAGCACGTTAAATTTGCTATCGTCCACTTCGCCAGTGGCATGTGCCCGCCCCAGTGCTTCACTAGCAAAACGTTCACTACCCAGCAACTGAATATTCGCCAGCGTCTGAGCTGCAAAGGCTTCGTGCATATCGAt            <  2:1163021/236‑1 (MQ=255)                                                                                                                                                                                                                            aCTAACACAAAC‑CCCCCGCCGCGACGGCGAAGTTCATGCAATGTCTGGGTAATCATCCGCGGGCCGGTCGCCGCGAAGAGATGCCCGTAAGCAATCGAACCGCCAAGCACGTTAAATTTGCTAGCGTCCACTTCGCCAGTGGCATGTGCACGCCCCAGTGCTTCACGAGCAAAACGTTCACTACCCAGCAACTGAATATTCGCCAGCGTCTGAGCTGCAAAGGCTTCGTGCATATCGAt            <  2:731851/239‑1 (MQ=255)                                                                                                                                                                                                                            aCTAACACAAAC‑CCCCCGCCGCGACGGCGAAGTTCAGGCAATGTCTGGGTAATCACCCGCGCGCCGGTCGCCGCGAAGGGATGCCCGTAAGCAATCGAACCGCCAAGCACGTTAAATTTGCTATCGTCCACTTCGCCAGTGGCATGTGCACGCCCCAGTGCTTCACGAGCAAAACGTTCACTACCCAGCAACTGAATATTCGCCAGCGTCTGAGCTGCAAAGGCTTCGTGCATATCGAt            <  2:677782/239‑1 (MQ=255)                                                                                                                                                                                                                            aCTAAACCAATT‑CCGCCCCCGCGACGGCGAAGTTCATGCAATGTCTGGGTAATCATCCGCGCGCCGGTCGCCGCGAAGGGATGCCCGAAGGCAATCGAACCGCCAAGCACGTTAAATTTGCTATCGTCCACTTCGCCAGTGGCATGTGCACGCCCCAGTGCTTCACGAGCAAAACGTTCACTACCCAGCAACTGAATATTCGCCAGCGTCTGAGCTGCAAAGGCTTCGTGCATATCGAt            <  2:169061/239‑1 (MQ=255)                                                                                                                                                                                                                            aCTAAACCAAAT‑CCCCCGCCGCGACGGCGAAGTTCATGCAATGTCTGGGTAATCATCCGCGCGCCGGTCGCCGCGAAGGGATGCCCGTAAGCAATCGAACCGCCAAGCACGTTAAATTTGCTATCGTCCACTTCGCCAGTGGCATGTGCACGCCCCAGTGCTTCACGAGCAAAACGTTCACTACCCAGCAACTGAATATTCGCCAGCGTCTGAGCTGCAAAGGCTTCGTGCATATCGAt            <  1:516742/239‑1 (MQ=255)                                                                                                                                                                                                                            aCTAAACCAAAC‑CCACCGCCGCGACGGCGAAGTTCAGGCAATGTCTGGGTAATCATCCGCGCGCCGGTCGCCGCGAAGGGATGCCCGTAAGCAATCGAACCGCCAAGCACGTTAAATTTGCTATCGTCCACTTCGCCAGTGGCATGTGCACGCCCCAGTGCTTCACGAGCAAAACGTTCACTACCCAGCAACTGAATATTCGCCAGCGTCTGAGCTGCAAAGGCTTCGTGCATATCGAt            <  1:334597/239‑1 (MQ=255)                                                                                                                                                                                                                            aCTAAACAAAAC‑CCCCCCCCGCGACGGCGAAGTTCATGCAATGTCTGGGTAATCATCCGCGCGCCGGTCGCCGCGAAGGGATGCCCGTAAGCAATCGAACCGCCAAGCACGTTAAATTTGCTATCGTCCACTTCGCCAGTGGCATGTGCACGCCCCAGTGCTTCACGAGCAAAACGTTCACTACCCAGCAACTGAATATTCGCCAGCGTCTGAGCTGCAAAGGCTTCGTGCATATCGAt            <  2:988657/239‑1 (MQ=255)                                                                                                                                                                                                                             acaaaaCAAATCCCCCCGCCGCGACGGCGAAGTTCATGCAATGTCTGGTTAATCATCCGCGCGCCGGTCGCCGCGAAGGGATGCCCGTAAGCAATCGAACCGCCAAGCACGTTAAATTTGCTATCGTCCACTTCGCCAGTGGCATGTGCACGCCCCAGTGCTTCACGAGCAAAACGTTCACTACCCAGCAACTGAATATTCGCCAGCGTCTGAGCTGCAAAGGCTTCGTGCATATCGAt            <  2:806809/237‑1 (MQ=255)                                                                                                                                                                                                                              tAACCCAACT‑CCCCCGCCGCGACGGCGAAGGTCATGCAATGGCTGGGTAATCACCCGCGCGCCGGTCTCCGCGAAGGGATGCCCGTAAGCAATCGAACCGCCAAGCACGTTAAATTTGCTATCGTCCACTTCGCCAGTGGCATGTGCACGCCCCAGTGCTTCACGAGCAAAACGTTCACTACCCAGCAACTGAATATTCGCCAGCGTCTGAGCTGCAAAGGCTTCGTGCATATCGATCa          <  2:951475/239‑1 (MQ=255)                                                                                                                                                                                                                              tAACCCAAAC‑CCGCCGCCGCGGCGGCGAAGTCAGGGGAATGGCTGGGTAATCAACCGCGCGCCGGTCGCCGGGAAGGGATGCCCGTAAGCAAGCGAACCGCCAAGCACGTTAAATTTGCTATCGTCCACTTCGCCAGTGGCATGTGCACGCCCCAGTGCTTGACGAGCAAAACGTTCACTACCCAGCAACTGAATATTCGCCAGCGTCTGAGCTGCAAAGGCTTCGTGCATATCGAt            <  2:719582/237‑1 (MQ=255)                                                                                                                                                                                                                               aaaccaaac‑cCCCCGCCGCGACGGCGAAGTTCATGCAATGTCTGGGTAATCATCCGCGCGCCGGTCGCCGCGAAGGGATGCCCGTAAGCAATCGAACCGCCAAGCACGTTAAATTTGCTATCGTCCACTTCGCCAGTGGCATGTGCACGCCCCAGTGCTTCACGAGCAAAACGTTCACTACCCAGCAACTGAATATTCGCCAGCGTCTGAGCTGCAAAGGCTTCGTGCATATCGATCaa         <  1:212964/239‑1 (MQ=255)                                                                                                                                                                                                                                aaCCAAAT‑CCACCGCCGCGACGGCGAAGTTCATGCAATGTCTGGGTAATCATCCGCGCGCCGGTCGCCGCGAAGGGATGCCCGTAAGCAATCGAACCGCCAAGCACGTTAAATTTGCTATCGTCCACTTCGCCAGTGGCATGTGCACGCCCCAGTGCTTCACGAGCAAAACGTTCACTACCCAGCAACTGAATATTCGCCAGCGTCTGAGCTGCAAAGGCTTCGTGCATATCGATCAAt        >  1:846210/1‑239 (MQ=255)                                                                                                                                                                                                                                aaCCAAAT‑CCACCGCCGCGACGGCGAAGTTCATGCAATGTCTGGGTAATCATCCGCGCGCCGGTCGCCGCGAAGGGATGCCCGTAAGCAATCGAACCGCCAAGCACGTTAAATTTGCTATCGTCCACTTCGCCAGTGGCATGTGCACGCCCCAGTGCTTCACGAGCAAAAAGTTCACTACCCAGCAACTGCATATTCGCCAGCGTCTGAGCTGCAAAGGCTTCGTGCATATCGATCAAt        >  2:12298/1‑239 (MQ=255)                                                                                                                                                                                                                                      aT‑CCACCGCCGCGACGGCGAAGTTCATGCAATGTCTGGGTAATCATCCGCGCGCCGGTCGCCGCGAAGGGATGCCCGTAAGCAATCGAACCGCCAAGCACGTTAAATTTGCTATCGTCCACTTCGCCAGTGGCATGTGCACGCCCCAGTGCTTCACGAGCAAAACGTTCACTACCCAGCAACTGAATATTCGCCAGCGTCTGAGCTGCAAAGGCTTCGTGCATATCGATCAATGTCAGa  >  1:441689/1‑239 (MQ=255)                                                                                                                                                                                                                                      aT‑CCACCGCCGCGACGGCGAAGTTCATGCAATGTCTGGGTAATCATCCGCGCGCCGGTCGCCGCGAAGGGATGCCCGTAAGCAATCGAACCGCCAAGCACGTTAAATTTGCTATCGTCCACTTCGCCAGTGGCATGTGCACGCCCCAGTGCTTCACGAGCAAAACGTTCACTACCCAGCAACTGAATATTCGCCAGCGCCTGCGCTGCAAAAGCATCGTGCATATCCATAAATATCa    >  2:514503/1‑237 (MQ=255)                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                               ACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGTACGTCGATGGTG‑ATAACGGCAATGTTGTCCAGACGAACATTAAGGGTAAACGCTGATGTCATTTCCATTATTCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGTAACTAAACCAAAT‑CCACCGCCGCGACGGCGAAGTTCATGCAATGTCTGGGTAATCATCCGCGCGCCGGTCGCCGCGAAGGGATGCCCGTAAGCAATCGAACCGCCAAGCACGTTAAATTTGCTATCGTCCACTTCGCCAGTGGCATGTGCACGCCCCAGTGCTTCACGAGCAAAACGTTCACTACCCAGCAACTGAATATTCGCCAGCGTCTGAGCTGCAAAGGCTTCGTGCATATCGATCAATGTCAGA  >  NC_000913/2458996‑2459462

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑