| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 2,464,499 | T→A | 12.3% | Y170F (TAC→TTC) | mlaA ← | ABC transporter maintaining OM lipid asymmetry, OM lipoprotein component |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,464,499 | 0 | T | A | 12.3% | 218.8 / 4.3 | 84 | Y170F (TAC→TTC) | mlaA | ABC transporter maintaining OM lipid asymmetry, OM lipoprotein component |
| Reads supporting (aligned to +/- strand): ref base T (44/28); new base A (2/9); total (47/37) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 9.85e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TTAAATCATCCTGAATCGCTTGTGCGTTCGGATTTTCCTGCGGTTTGAGTTCGCCGCCATTAGCGATGAAATCATGACGCTGGAAGTACGCTTCGCGCACCATAATAT‑AAGGATCGGACGACTGACGCAGCAGACCATCGGAATCCAGCAGCTGAGCGCGGGTTTCGATCCCTTCAAGCGTCCATTTACCCACAGACATCGGCCAGGTCAGCCAGGAAAGAACCGGGTAAAAACCATCCGCCATATCACCACCGTCATCACGCAGCGTGAAGCTACCGTAGAACGGTAACTGAACGTAAGGCCCATAACCCACGCCATAATGACCAAGCGTACTACCGAAGCGGTGAGGTTCAGTCCGTTGCAGTTTCGGGTTCGCCATCCCTGCAACATCAATAAAACCGCCCATCCCCAAAATGGTGTTCAGGAAAAAGCGGGTAAAGTGGACCATCCCCTGAT > NC_000913/2464272‑2464727 | ttAAATCATCCTGAATCGCTTGTGCGTTCGGATTTTCCTGCGGTTTGAGTTCGCCGCCATTAGCGATGAAATCATGACGCTGGAAGTACGCTTCGCGCACCATAATAT‑AAGGATCGGACGACTGACGCAGCAGACCATCGGAATCCAGCAGCTGAGCGCGGGTTTCGATCCCTTCAAGCGTCCATTTACCCACAGACATCGGCCAGGTCAGCCCGGAAAGAACCGGGTAAAAACCATcc > 1:621369/1‑239 (MQ=255) tAAATCATCCTGAATCGCTTGTGCGTTCGGATTTTCCTGCGGTTTGAGTTCGCCGCCATTAGCGATGAAATCATGACGCTGGAAGTACGCTTCGCGCACCATAATAT‑AAGGATCGGACGACTGACGCAGCAGACCATCGGAATCCAGCAGCTGAGCGCGGGATTCGATCCCTTCAAGCGTCCATTTACCCACAGACATCGGCGAGGTCAGCCAGGAAAGAACCGGGTAAAAACCATcc > 2:516227/1‑238 (MQ=255) aaaTCATCCTGAACCGGTTGGGGGTACGAATTTCCCGGCGGTTGGAGTTCGCCGCCATTAGCGATGAAATCATGACGCTGGAATTACGCTCCGCGCACCATAATAT‑AAGGATCGGACGACTGACGCAGCAGACCATCGGAATCCAGCAGCTGAGCGCGGGTTTGGATCCCTCAAAGCGTCCATTTACCAACAGACATCGGCCACGTCAGCCAGGAAAGAACCGGGTAAAAACCATCCGc < 2:562510/239‑1 (MQ=255) tcCTGAATCGCTTGTGCGTTCGGATTTTCCTGCGGTTTGAGTTCGCCGCCATTAGCGATGAAATCATGACGCTGGAAGTACGCTTCGCGCACCATAATAT‑AAGGATCGGACGACTGACGCAGCAGACCATCGGAATCCAGCAGCTGAGCGCGGGTTTCGATCCCTTCAAGCGTCCATTTACCCACAGACATCGGCCAGGTCAGCCAGGAAAGAACCGGGTAAAAACCATCCGCCATATc > 1:117747/1‑239 (MQ=255) tcCTGAATCGCTTGTGCGTTCGGATTTTCCTGCGGTTTGAGTTCGCCGCCATTAGCGATGAAATCATGACGCTGGAAGTACGCTTCGCGCACCATAATAT‑AAGGATCGGACGACTGACGCAGCAGACCATCGGAATCCAGCAGCTGAGCGCGGGTTTCGATCCCTTCAAGCGTCCATTTACCCACAGACATCGGCCAGGTCAGCCAGGAAAGAACCGGGAAAAAACCATCCGCCatat > 1:537842/1‑238 (MQ=255) ccTGAATCGCTTGTGCGTTCGGATTTTCCTGCGGTTTGAGTTCGCCGCCATTAGCGATGAAATCATGACGCTGGAAGTACGCTTCGCGCACCATAATAT‑AAGGATCGGACGACTGACGCAGCAGACCATCGGAATCCAGCAGCTGAGCGCGGGTTTCGATCCCTTCAAGCGTCCATTTACCCACAGACATCGCCCATGTCAGCCAGGAAAGAACCGGGTCAAAACCATCCGCCATATc > 1:556781/1‑238 (MQ=255) aaTCGCTTGTGCGTTCGGATTTTCCTGCGGTTTGAGTTCGCCGCCATTAGCGATGAAATCATGACGCTGGAAGTACGCTTCGCGCACCATAATAT‑AAGGATCGGACGACTGACGCAGCAGACCATCGGAATCCAGCAGCTGAGCGCGGGTTTCGATCCCTTCAAGCGTCCATTTACCCACAGACATCGGCCAGGTCAGCCCTGCAAGAACCGGGTAAACACCACCCGCACTATcaccc > 2:538462/1‑237 (MQ=255) aaTCGCTTGTGCGTTCGGATTTTCCTGCGGGTTGAGTTCGCCGCCATTAGCGATGAAATCATGACGCTGGAAGTACGCTTCGCGCACCATAATAT‑AAGGATCGGACGACTGACGCAGCAGACCATCGGAATCCAGCAGCTGAGCGCGGGTTTCGATCCCTTCAAGCGTCCATTTACCCACAGACATCGGCCAGGTCAGCCAGGAAAGAACCGGGAACAACCCATCCCCCATATcacca > 2:462376/1‑238 (MQ=255) tttGTGCGTACCGTTTTCCCGGCAGTTAGAGTCCGCCGCAATTAGCGATGAAATCATGACGCTGGAAGTACGCTTCGCGCCCCATAATAT‑AAGGATCGGACGCCTGCCGCAGCAGACCATCGGAATCCAGCAGCTGAGCGCGGGTTTCGATCCCTTCAAGCGTCCATTTACCCACAGCCATCGGCCAGGTCAGCCAGGAAAGAACCGGTTAAAAACCATCCGCCATATCACCACCGtc < 2:425366/237‑1 (MQ=255) cTTGTGCGTTCGGATTTTCCTGCGGTTTGAGTTCGCCGCCATTAGCGATGAAATCATGACGCTGGAAGTACGCTTCGCGCACCATAATAT‑AAGGATCGGACGACTGACGCAGCAGACCATCGGAATCCAGCAGCTGAGCGCGGGTTTCGATCCCTTCAAGCGTCCATTTACCCACAGACATCGGCCAGGTCAGCCAGGAAAGAACCGGGTAAAAACCATCCGCCATATCACCACCGtca > 1:1113600/1‑239 (MQ=255) cttccGGATTTGCCTGCGGTTGGAGTTCGCCGCCATTAGCAATGAAATCATGACGCTGGAAGTACGCTTCGCGCACCATAATAT‑AAGGATCGGACGACTGACGCAGCAGACCATCGGAATCCAGCAGCTGAGCGCGGGTTTCGATCCCTTCAAGCGTCCATTTACCCACAGACAGCGGCCAGGTCAGCCAGGAAAGAACCGGGTAAAAACCATCCGCCATATCACCACCGTCATCACg < 1:272663/234‑1 (MQ=255) cggacGGAGTTTCCCGCGGTTGAGGTCCCCCGCCATTAGCGATAAAATCATGACGCGGAAAGTCCGCTTCGCGCAACATAAGAT‑AAGGATCGGACGACTGCCGCAGCAGACCCTCGGGATCCAGCAGCTGAGCGCGGGTTTCGATCCCTTCAAGCGTCCATTAACCCCCAGACATCGGCCAGGCCAGCCAGGAAAGAACCGGGTAAAACCCATCCGCCATATCACCACCGTCATCACg < 2:1013324/234‑1 (MQ=255) gTTCGGATTTTCCTGCGGTTTGAGTTCGCCGCCATTAGCGATGAAATCATGACGCTGGAAGTACGCTTCGCGCACCATAATAT‑AAGGATCGGACGACTGACGCAGCAGACCATCGGAATCCAGCAGCTGAGCGCGGGTTTCGATCCCTTCAAGCGTCCATTTACCCACAGACATCGGCCAGGTCAGCCAGGAAAGAACCGGGTAAAAACCATCCGCCATATCACCACCGTCATCACGc < 1:22810/238‑1 (MQ=255) gATTTCTCTGCAGTAGGACTTCGCCAGCATTAGCGATGAAATCAAGACGATGGAAGTACGCTTCGCGAACCATAAGAG‑AAGCATGGGGCGCCGAACGCACGAGAACAGCGGAATCCAGCAGCTGAGCGCGGGTTGCGACCCCTTAAAGTGTCAATTTACCCACAGCCATCGCCCAGGTCCGCCAGGAAAGAACCGGGTAAAAACCATCCGCCATATCACCACCGTCATCACGCAGCgg < 2:488183/238‑2 (MQ=255) cagTTTGAGTTCGCCGCCAATAGCGATGAAATCCTGACGCTGGAAGTACACTTCGCGCACCATAATAT‑AAGGATCGGACGACTGACGCAGCAGATCATCGGAATCCAACAGCTGAGCGCGGGGTTCGATCCCTTCAAGCGGCCATTTACCAACAGACAGCGGACAGGTCAGCCAGGAAAGAACCGGGCAAAAACCATCCGCCAGATCACCACCGCCATCACGAAGCGGGAAGCTACCga > 2:248880/3‑238 (MQ=255) tgtGATTTAGCGGCCATTAGCGAAAAAAACAGGACGCGGGAAGTACCCCTCGCGCAACATAAAAT‑AAGGGTCGGACGACTGACGCAGCAGACCAGCGAAATCCAGCAGCTGAGCGCGGGTTGCAACCCCTAAAAGCGTCCATTTACCCACAGACATCGGCCAGGCCAGCCAGGAAAGAACCGGGTAAAAACCATCCGCCATATCACCACCGTCATCACGCAGCGTGAAGCTACCGTAg < 2:621369/236‑1 (MQ=255) tGAGTTCGCCGCCATTAGCGATGAAATCATGACGCTGGAAGTACGCTTCGCGCACCATAATAT‑AAGGATCGGACGACTGACGCAGCAGACCATCGGAATCCAGCAGCTGAGCGCGGGTTTCGATCCCTTCAAGCGTCCATTTACCCACAGACATCGGCCAGGTCAGCCAGGAAAGAACCGGGTAAAAACCATCCGCCATATCACCACCGTCATCACGCAGCGTGAAGCTACCGTAGaa > 2:375959/1‑238 (MQ=255) atttCGCCCCCATTAGGAATAAAAGCATGCCGTGGGAAGTACGCTTCGCGCACCATAATAT‑AAGGATCGGACGACTGACGCAGCAGACCATCGGAATCCAGCAGCGGAGCGCGGGTTTCGATCCCTTCAAGCGTCCATTTACCCACAGCCACCGGCCAGGTCCGCCAGGAAAGACCCGGGTAAAAACCATCCGCCATATCACCACCGTCATCACGCAGCGTGAAGCTACCGTAGAACg < 2:301975/236‑1 (MQ=255) cgccgcCATTAGCGATGAAATCATGACGCTGGAAGTACGCTTCGCGCACCATAATAT‑AAGGATCGGACGACTGACGCAGCAGACCATCGGAATCCAGCAGCTGAGCGCGGGTTTCGATCCCTTCAAGCGTCCATTTACCCACAGACATCGGCCAGGTCAGCCAGGAAAGAACCGGGTAAAAACCATCCGCCATATCACCACCGTCATCACGCAGCGTGAAGCTACCGTAGAACGGTaa > 2:601326/1‑238 (MQ=255) ccgccATTAGCGATGAAATCATGACGCTGGAAGTACGCTTCGCGCACCATAATAT‑AAGGATCGGACGACTGACGCAGCAGACCATCGGAATCCAGCAGCTGAGCGCGGGTTTCGATCCCTTCAAGCGTCCATTTACCCACAGACATCGGCCAGGTCAGCCAGGAAAGAACCGGGTAAAAACCATCCGCCATATCACCACCGTCATCACGCAGCGTGAAGCTACCGTAGAACGGTAACTg > 1:315977/1‑239 (MQ=255) ttAGCGATGAAATCATGACGCTGGAAGTACGCTTCGCGCACCATAATAT‑AAGGATCGGACGACTGACGCAGCAGACCATCGGAATCCAGCAGCTGAGCGCGGGTTTCGATCCCTTCAAGCGTCCATTTACCCACAGACATCGGCCAGGTCAGCCAGGAAAGAACCGGGTAAAAACCATCCGCCATATCACCACCGTCATCACGCAGCGTGAAGCTACCGTAGAACGGTAACTGAACGTa < 1:583854/239‑1 (MQ=255) taaGCGATGAAACAATGCCGTGGCAAGTACGCGTCCCGCCCCATAAAAG‑AAGGATCGGACCACTGACGCTGCAGCCCATCGGAATCCACCAGCGGAGGGGGGGTTTCGAACCCTTCAACCGGCCCTTTACCCCCAGACACCGGCCAGGCAAGCCAGGAAAGAACCGGGTAAAAACCATCCGCCATATCACCACCGTCATCACGCAGCGTGAAGCTACCGTAGAACGGTAACTGAACGTa < 2:398212/237‑1 (MQ=255) tGAAATCATGACGCTGGAAGTACGCGTCGCGCACCATAATAT‑AAGGATCGGACGACTGACGCAGCAGACCATCGGAATCCAGCAGCTGAGCGCGGGTTTCGATCCCTTCAAGCGTCCATTTACCCACAGACATCGGCCAGGTCAGCCAGGAAAGAACCGGGTAAAAACCATCCGCCATATCACCACCGTCATCACGCAGCGTGAAGCTACCGTAGAACGGTAACTGAACGTAAGGCCCa < 1:710839/239‑1 (MQ=255) aCGCTGGAAGTACGCTTCGCGCACCATAATAT‑AAGGATCGGACGACTGACGCAGCAGACCATCGGAATCCAGCAGCTGAGCGCGGGTTTCGATCCCTTCAAGCGTCCATTTACCCACAGACATCGACCAGGTCAGCCAGGAAAGAACCGGGTAAAAACCATCCGCCATATCACCACCGTCATCACGCAGCGTGAAGCTACCGTAGAACGGTAACTGAACGTAAGGCCCATAACCCACg > 2:319740/1‑238 (MQ=255) ggAAGTACCCTTCGCGCCCCATAATAT‑AAGGATCGGACGACTCACGCAGCAGACCATCGGAATCAAGCAGCTGAGCGCGGGTTTCGATCCCTTCAAGCGTCCATTTCCCCACAGACATCGGCCAGGTCAGCCAGAAAAGAACCGGGTAAAAACCATCCGCCATATCACCACCGTCATCACGCAGCGTGAAGCTACCGTAGAACGGTAACTGAACGTAAGGCCCATAACCCACGCCATa < 1:550436/238‑1 (MQ=255) gAAGTACGCTTCGCGCACCATAATAT‑AAGGATCGGACGACTGACGCAGCAGACCATCGGAATCCAGCAGCTGAGCGCGGGTTTCGATCCCTTCAAGCGTCCATTTACCCACAGACATCGGCCAGGTCAGCCAGGAAAGAACCGGGTAAAAACCATCCGCCATATCACCACCGTCATCACGCAGCGTGAAGCTACCGTAGAACGGTAACTGAACGTAAGGCCCATAACCCACGCCATAAt > 2:330154/1‑239 (MQ=255) gccacacaaataTAAAGGACCGGAGCACGGACCCCCCAGACCATCGGAACCCAGCAGCGACGGCCGGGTTTGGATCCCTACAAGCGTCCATTACCCCACAGACAGCGCCCAGGTCAGCAAGAAAAGAACCGGTTAAAAACCATCCGCCATATCACCACCGTCATCACGCAGCGTGAAGCTACCGTAGAACGGTAACTGAACGTAAGGCCCATAACCCACGCCATAATGACCAAGCGtac < 2:1113600/231‑1 (MQ=255) cATAATAT‑AAGGATCGGACGACTGACGCAGCAGACCATCGGAATCCAGCAGCTGAGCGCGGGTTTCGATCCCTTCAAGCGTCCATTTACCCACAGACACCGGCCAGGTCAGCCAGGAAAGACCCGGGTAAAACCCATCCGCCATATCACCACCGTCATCACGCAGCGTGAAGCTACCGTAGAACGGTAACTGAACGTAAGGCCCATAACCCACGCCATAATGACCAAGCGTACTACCGa < 1:375683/239‑1 (MQ=255) tat‑aAGGATCGGACGACTGACGCAGCAGACCATCGGAATCCAGCAGCTGAGCGCGGGTTTCGATCCCTTCAAGCGTCCATTTACCCACAGACATCGGCCAGGTCAGCCAGGAAAGAACCGGGTAAAAACCATCCGCCATATCACCACCGTCATCACGCAGCGTGAAGCTACCGTAGAACGGTAACTGAACGTAAGGCCCATAACCCACGCCATAATGACCAAGCGTACTACCGAAGCgg > 1:1157818/1‑239 (MQ=255) aaGGCTCGGACGCCTGACTCAGCAGACCATCGGAACCCAGCAGCTACGCGCGGGTTTCGATCCCTTCAACCGTCCTTTTACCCACAGACACCGGCCAGCCCAGCCAGGAAAGAACCGGGAAAAACCCATCCGCCATATCACCACCGTCATCACGCAGCGTGAAGCTACCGTAGAACGGTAACTGAACGTAAGGCCCATAACCCACGCCATAATGACCAAGCGTACTACCGAAGCGGTGa < 2:68157/239‑1 (MQ=255) aaGAATCGGACGACTGACGCAGCAGACCATCGGAATCCAGCAGCTGAGCGCGGGTTTGGATCCCTTCAAGCGTCCATTTACCCACAGACATCGGCCAGGTCAGCCAGGAAAGAACCGGGTAACAACCATCCGCCATATCACCACCGTCATCACGCAGCGTGAAGCAACCGTAGAACGGAAACTAAACGTAACGCCCATAACCCACGCCATACTGACCAAGCGAACTACCGAAGGGGCGa > 2:604825/1‑239 (MQ=255) ggATCGGACGACTGCCGCAGCAGACCATCGGAATCCAGCAGCTGAGCGCGGGTTTCGATCCCTTCACGCGTCCATTTACCCACAGACATCGGCCAGGTCAGCCAGGAAAGAACCGGGTAAAAACCATCCGCCATATCACCACCGTCATCACGCAGCGTGAAGCTACCGTAGAACGGTAACTGAACGTAAGGCCCATAACCCACGCCATAATGACCAAGCGTACTACCGAAGCGGTGAgg > 2:136255/1‑239 (MQ=255) ggATCGGACGACTGACGCAGCAGACCATCGGAATCCAGCAGCTGAGCGCGGGTTTCGATCCCTTCAAGCGTCCATTTACCCACAGACATCGGCCAGGTCAGCCAGGAAAGAACCGGGTAAAAACCATCCGCCATATCACCACCGTCATCACGCAGCGTGAAGCTACCGTAGAACGGTAACTGAACGTAAGGCCCATAACCCACGCCATAATGACCAAGCGTACTACCGAAGCGGTGAgg > 1:717378/1‑239 (MQ=255) ggATCGGACGACTGACGCAGCAGACCATCGGAATCCAGCAGCTGAGCGCGGGTTTCGATCCCTTCAAGCGTCCATTTACCCACAGACATCGGCCAGGTCAGCCAGGAAAGAACCGGGTAAAAACCATCCGCCATATCACCACCGTCATCACGCAGCGTGAAGCTACCGTAGAACGGTAACTGAACGTAAGGCCCATAACCCACGCCATAATGACCAAGCGTACTACCGAAGCGGTGAgg > 1:749265/1‑239 (MQ=255) ggATCGGACGACTGACGCAGCAGACCATCGGAATCCAGCAGCTGAGCGCGGGTTTCGATCCCTTCAAGCGTCCATTTACCCACAGACATCGGCCAGGTCAGCCAGGAAAGAACCGGGTAAAAACCATCCGCCATATCACCACCGTCATCACGCAGCGTGAAGCTACCGTAGAACGGTAACTGAACGTAAGGCCCATAACCCACGCCATAATGACCAAGCGTACTACCGAAGCGGTGAgg > 1:55234/1‑239 (MQ=255) ggATCGGACGACTGACGCAGCAGACCATCGGAATCCAGCAGCTGAGCGCGGGTTTCGATCCCTTCAAGCGTCCATTTACCCACAGACATCGGCCAGGTCAGCCAGGAAAGAACCGGGTAAAAACCATCCGCCATATCACCACCGTCATCACGCAGCGTGAAGCCACCGTAGAACGGTAACTGAACGTAAGGCCCATAACCCACGCCATAATGACCAAGCGTACTACCGAAGCGGTGAgg > 1:1131057/1‑239 (MQ=255) aTCGGACGACTGACGCAGCAGACCATCGGAATCCAGCAGCTGAGCGCGGGTTTCGATCCCTTCAAGCGTCCATTTACCCACAGACATCGGCCAGGTCAGCCAGGAAAGAACCGGGTAAAAACCATCCGCCATATCACCACCGTCATCACGCAGCGTGAAGCTACCGTAGAACGGTAACTGAACGTAAGGCCCATAACCCACGCCATAATGACCAAGCGTACTACCGAAGCGGTGAGGt > 1:779089/1‑238 (MQ=255) aTCGGACGACTGACGCAGCAGACCATCGGAATCCAGCAGCTGAGCGCGGGTTTCGATCCCTTCAAGCGTCCATTTACCCACAGACATCGGCCAGGTCAGCCAGGAAAGAACCGGGTAAAAACCATCCGCCATATCACCACCGTCATCACGCAGCGTGAAGCTACCGTAGAACGGTAACTGAACGTAAGGCCCATAACCCACGCCATAATGACCAAGCGTACTACCGAAGCGGTGAGGt > 1:18733/1‑238 (MQ=255) tCGGACGACTGACGCAGCAGACCATCGGAATCCAGCAGCTGAGCGCGGGTTTCGATCCCTTCAAGCGTCCATTTACCCACAGACATCGGCCAGGTCAGCCAGGAAAGAACCGGGTAAAAACCATCCGCCATATCACCACCGTCATCACGCAGCGTGAAGCTACCGTAGAACGGTAACTGAACGTAAGGCCCATAACCCACGCCATAATGACCAAGCGTACTACCGAAGCGGTGAGGTTc > 1:760493/1‑239 (MQ=255) cGGACGACTGACGCAGCAGACCATCGGAATCCAGCAGCTGAGCGCGGGTTTCGATCCCTTCAAGCGTCCATTTACCCACAGACATCGGCCAGGTCAGCCAGGAAAGAACCGGGTAAAACCCATCCGCCATATCACCACCGTCATCACg < 2:903692/148‑1 (MQ=255) cGGACGACTGACGCAGCAGACCATCGGAATCCAGCAGCTGAGCGCGGGTTTCGATCCCTTCAAGCGTCCATTTACCCACAGACATCGGCCAGGTCAGCCAGGAAAGAACCGGGTAAAAACCATCCGCCATATCACCACCGTCATCACg > 1:903692/1‑148 (MQ=255) cGGACGACTGACGCAGCAGACCATCGGAATCCAGCAGCTGAGCGCGGGTTTCGATCCCTTCAAGCGTCCATTTACCCACAGACATCGGCCAGGTCAGCCAGGAAAGAACCGGGTAAAAACCATCCGCCATATCACCACCGTCATCACGCAGCGTGAAGCTACCGTAGAACGGCAACTGACCGGAAGGCCCATAACCCCCGCCATAATGCCCAAGCGTACTACCGCACCGCTGAGGTTc > 2:801638/1‑238 (MQ=255) gacgacTGACGCAGCAGACCATCGGAATCCAGCAGCTGAGCGCGGGTTTCGATCCCTTCAAGCGTCCATTTACCCACAGACATCGGCCAGGTCAGCCAGGAAAGAACCGGGTAAAAACCATCCGCCATATCACCACCGTCATCACGCAGCGTGAAGCTACCGTAGAACGGTAACt > 2:307197/1‑175 (MQ=255) gacgacTGACGCAGCAGACCATCGGAATCCAGCAGCTGAGCGCGGGTTTCGATCCCTTCAAGCGTCCATTTACCCACAGACATCGGCCAGGTCAGCCAGGAAAGAACCGGGTAAAAACCATCCGCCATATCACCACCGTCATCACGCAGCGTGAAGCTACCGTAGAACGGTAACt < 1:307197/175‑1 (MQ=255) tGACGCAGCAGACCATCGGAATCCAGCAGCTGAGCGCGGGTTTCGATCCCTTCAAGCGTCCATTTACCCACAGACATCGGCCAGGTCAGCCAGGAAAGAACCGGGTAAAAACCATCAGCCATATCACCACCGTCATCACGCAGCGTGAAGCTACCGTAGAACGGTAACTGAACGTAAGGCCCATaa > 1:71118/1‑186 (MQ=255) ccATCGGAATCCAGCAGCTGAGCGCGGGTTTCGATCCCTTCAAGCGTCCATTTACCCACAGACATCGGCCAGGTCAGCCAGGAAAGAACCGGGTAAAAACCATCCGCCATATCACCACCGTCATCACGCAGCGTGAAGCTACCGTAGAACGGTAACTGAACGTAAGGCCCATAACCCACGCCATAATGACCAAGCGTACTACCGAAGCGGTGAGGTTCAGTCCGTTGCAGTTTCgg < 1:601326/236‑1 (MQ=255) cGGAATCCAGCAGCGGAGCGCGGGTTTCGATCCCTTAAAGCGTCCATTTACCCACAGACATCGGCCAGGTCAGCCAGGAAAGAACCGGGTAAAAACCATCCGCCATATCACCACCGTCATCACGCAGCGTGAAGCTACCGTAGAACGGTAACTGAACGTAAGGCCCATAACCCACGCCATAATGACCAAGCGTACTACCGAAGCGGTGAGGTTCAGTCCGTTGCAGTTTCGGGTTCGcc < 1:731474/239‑1 (MQ=255) accccAGCAGCCGAGTGCGGGTTTCGAACCACTCAAGCGTCAATTAACCCCCAGCCATCGGCCAGGCCAGCCAGGAAAGAACCGGGTAAAAACCATCCGCCATATCACCACCGTCATCACGCAGCGTGAAGCTACCGTAGAACGGTAACTGAACGTAAGGCCCATAACCCACGCCATAATGACCAAGCGTACTACCGAAGCGGTGAGGTTCAGTCCGTTGCAGTTTCGGGTTCGCCAt < 2:537842/235‑1 (MQ=255) tCCAGCAGCTGAGCGCGGGTTTCGATCCCTTCAAGCGTCCATTTACCCACAGACATCGGCCAGGTCAGCCAGGAAAGAACCGGGTAAAAACCATCCGCCATATCACCACCGTCATCACGCAGCGTGAAGCTACCGTAGAACGGTAACTGAACGTAAGGCCCATAACCCACGCCATAATGACCAAGCGTACTACCGAAGCGGTGAGGTTCAGTCCGTTGCAGTTTCGGGTTCGCCATc > 1:878593/1‑237 (MQ=255) tCCAGCAGCTGAGCGCGGGTTTCGATCCCTTCAAGCGTCCATTTACCCACAGACATCGGCCAGGTCAGCCAGGAAAGAACCGGGTAAAAACCATCCGCCATATCACCACCGTCATCACGCAGCGTGAAGCTACCGTAGAACGGTAACTGAACGTAAGGCCCATAACCCACGCCATAATGACCAAGCGTACTACCGAAGCGGTGAGGTTCAGTCCGTTGCAGTTTCGGGTTCGCCATc > 2:92151/1‑237 (MQ=255) tCCAGCAGCTGAGCGCGGGTTTCGATCCCTTCAAGCGTCCATTTAACCACAGACATCGGCCAGGTAAGCCAGGAAAGAACCGGGTAAAAACCATCCGCCATATCACCACCGTCATCACGCAGCGTGAAGCTACCGTAGAACAGTACCTGAACGTAAGGCCCATAACCCACGCCCTAATGACCAAGCGTAATACCGAAGCGGTGAGGTTAAGTCCGTTGCAGTTTCGGGATCGCCATc > 1:487109/1‑237 (MQ=255) cagcTGAGCGCGGGTTTCGATCCCTTCAAGCGTCCATTTACCCACAGACATCGGCCAGGTCAGCCAGGAAAGAACCGGGTAAAAACCATCCGCCATATCACCACCGTCATCACGCAGCGTGAAGCTACCGTAGAACGGTAACTGAACGTAAGGCCCATAACCCACGCCATAATGACCAAGCGTACTACCGAAGCGGTGAGGTTCAGTCCGTTGCAGTTTCGGGTTCGCCATCCCTGCa > 1:1138031/1‑238 (MQ=255) cagcTGAGCGCGGGTTTCGATCCCTTCAAGCGTCCATTTACCCACAGACATCGGCCAGGTCAGCCAGGAAAGAACCGGGTAAAAACCATCCGCCATATCACCACCGTCATCACGCAGCGTGAAGCTACCGTAGAACGGTAACTGAACGTAAGGCCCATAACCCACGCCATAATGACCAAGCGTACTACCGAAGCGGTGAGGTTCAGTCCGTTGCAGTTTCGGGTTCGCCATCCCTGCa > 1:495029/1‑238 (MQ=255) cagcTGAGCGCGGGTTTCGATCCCTTCAAGCGTCCATTTACCCACAGACATCGGCCAGGTCAGCCAGGAAAGAACCGGGTAAAAACCATCCGCCATATCACCACCGTCATCACGCAGCGTGAAGCTACCGTAGAACGGTAACTGAACGTAAGGCCCATAACCCACGCCATAATGACCAAACGTACTACCGAAGCGGTGAGGTTCAGTCCGTTGCAGTTTCGGGTTCGCCATCCCTGCa > 1:705880/1‑238 (MQ=255) ccTTAAAGCGTCCATTTACCCACATACATCGACCAGGTCGCCCAGGAAAGAACCGGGAAAAACCCATCCGCCATATCACCACCGTCATCACGCAGCGTGAAGCTACCGTAGAACGGTAACTGAACGTAAGGCCCATAACCCACGCCATAATGACCAAGCGTACTACCGAAGCGGTGAGGTTCAGTCCGTTGCAGTTTCGGGTTCGCCATCCCTGCAACATCAATAAAACCGCCCATccc < 1:252808/239‑1 (MQ=255) tCCATTTACCCACAGACATCGGCCAGGTCAGCCAGGAAAGAACCGGGTAAAAACCATCCGCCATATCACCACCGTCATCACGCAGCGTGAAGCTACCGTAGAACGGTAACTGAACGCAAGGCCCATAACCCACGCCATAATGACCAAGCGTACTACCGAAGCGGTGAGGTTCAGTCCGTTGCAGTTTCGGGTTCGCCATCCCTGCAACATCAATAAAACCGCCCATCCCCAAAATggcg > 1:504312/1‑237 (MQ=255) tCCATTTACCCACAGACATCGGCCAGGTCAGCCAGGAAAGAACCGGGTAAAAACCATCCGCCATATCACCACCGTCATCACGCAGCGTGAAGCTACCGTAGAACGGAAACTGAACGTAAGGCCCATAACCCACGCCATAATGACCAAGCGTACTACCGAAGCGGTGAGGTTCAGTCCGTTGCAGTTTCGGGTTCGCCATCCCTGCAACATCAATAACACCGCCCATCCCCAAAATGgtg > 1:426316/1‑239 (MQ=255) caaTTAACCCCCAGCCACCGGCCAGGCCAGCAAGAAAAGAACCGGGAAAAACCCATCCGCCATATCACCACCGACAGCACGCAGCGGGAAGCTACCGTAGAACGGTAACTGAACGTAAGGCCCATAACCCACGCCATAATGACCAAGCGTACTACCGAAGCGGTGAGGTTCAGTCCGTTGCAGTTTCGGGTTCGCCATCCCTGCAACATCAATAAAACCGCCCATCCCCAAAATGgtgt < 2:779089/237‑1 (MQ=255) cATTTACCCACAGACATCGGCCAGGTCAGCCAGGAAAGAACCGGGTAAAAACCATCCGCCATATCACCACCGTCATCACGCAGCGTGAAGCTACCGTAGAACGGTAACTGAACGTAAGGCCCATAACCCACGCCATAATGACCAAGCGTACTACCGAAGCGGTGAGGTTCAGTCCGTTGCAGTTTCGGGTTCGCCATCCCTGCAACATCAATAAAACCGCCCATCCCCAAAATGgtgt > 2:1063137/1‑238 (MQ=255) cATTTACCCACAGACATCGGCCAGGTCAGCCAGGAAAGAACCGGGTAAAAACCATCCGCCATATCACCACCGTCATCACGCAGCGTGAAGCTACCGTAGAACGGTAACTGAACGTAAGGCCCATAACCCACGCCATAATGACCAAGCGTACTACCGAAGCGGTGAGGTTCAGTCCGTTGCAGTTTCGGGTTCGCCATCCCTGCAACATCAATAAAACCGCCCATCCCCAAAATGgtgt > 1:105126/1‑238 (MQ=255) acaGACATCGGCCAGGTCAGCCAGGAAAGAATCGGGTAAAAACCATCCGACTTATCACCACCGTAATCACGCAGCGTGAAGCTACCGTAGAACGGTAAATGAACGTAAGGCCCATAACCCAAGCCATAATGACCAAGCCTACTAACGAAGTGGTAAGGCTCAGTCAGATGCAGATTCGGGTTCGCCATCCCGGCAACATCAATAAAACCGCCCATCCCCAAACTGGTGTTCGGGaaaaa > 2:1054758/1‑239 (MQ=255) aGACATCGGCCAGGTCAGCCAGGAAAGAACCGGGTAAAAACCATCCGCCATATCACCACCGTCATCACGCAGCGTGAAGCTACCGTAGAACGGTAACTGAACGTAAGGCCCATAACCCACGCCATAATGACCAAGCGTACTACCGAAGCGGTGAGGTTCAGTCCGTTGCAGTTTCGGGTTCGCCATCCCTGCAACATCAATAAAACCGCCCATCCCCAAAATGGTGTTCAGGAAAAAGc > 1:191593/1‑239 (MQ=255) aGACATCGGCCAGGTCAGCCAGGAAAGAACCGGGTAAAAACCATCCGCCATATCACCACCGTCATCACGCAGCGTGAAGCTACCGTAGAACGGTAACTGAACGTAAGGCCCATAACCCACGCCATAATGACCAAGCGTACTACCGAAGCGGCGAGGTTCAGTCCGTTGCAGTTTCGGGTTCGCCATCCCTGCAACATCAATAAAACCGCCCATCCCCAAAATGGTGTTCCGGAAAAAGc > 1:893644/1‑239 (MQ=255) tCGGCCAGGTCAGCCAGGAAAGAACCGGGTAAAAACCATCCGCCATATCACCACCGTCATCACGCAGCGTGAAGCTACCGTAGAACGGTAACTGAACGTAAGGCCCATAACCCACGCCATAATGACCAAGCGTACTACCGAAGCGGTGAGGTTCAGTCCGTTGCAGTTTCGGGTTCGCCATCCCTGCAACATCAATAAAACCGCCCATCCCCAAAATGGTGTTCAGGAAAAAGCGGGTa > 2:606640/1‑239 (MQ=255) cGGCCAGGTCAGCCAGGAAAGAACCGGGTAAAAACCATCCGCCATATCACCACCGTCATCACGCAGCGTGAAGCTACCGTAGAACGGTAACTGAACGTAAGGCCCATAACCCACGCCATAATGACCAAGCGTACTACCGAAGCGGTGAGGTTCAGTCCGTTGCAGTTTCGGGTTCGCCATCCCTGCAACATCAATAAAACCGCCCATCCCCACAATGGTGTTCAGGAAAAAGCGGGTaa > 1:635538/1‑239 (MQ=255) gcccggctcgcccAGGAAAGACCCGGGAAAACCCCACCCGCCATACCACCACCGCCACCACGCAGCGTGAAGCGACCGTAGAACGGTAACTGAACGAAAGGCCCAGAACCCACGCCATAATGACCAAGCGTACTAACGAAGCGGTGAGGTTCAGTCCGTTGCAGTTTCGGGTTCGCCATCCCTGCAACATCAATAAAACCGCCCATCCCCAAAATGGTGTTCAGGAAAAAGCGGGTaaa < 2:920515/228‑1 (MQ=255) gaccAGGTCAGCCAGGAAAGAACCGGGTAAAAACCATCCGCCATATCACCACCGTCATCACGCAGCGTGAAGCTACCGTAGAACGGTAACTGAACGTAAGGCCCATAACCCACGCCATAATGACCAAGCGTACTACCGAAGCGGTGAGGTTCAGTCCGTTGCAGTTTCGGGTTCGCCATCCCTGCAACATCAATAAAACCGCCCATCCCCAAAATGGTGTTCAGGAAAAAGCGGGTaaa < 1:319740/237‑1 (MQ=255) ggTCAGCCAGGAAAGAACCGGGTAAAAACCATCCGCCATATCACCACCGTCATCACGCAGCGTGAAGCTACCGTAGAACGGTAACTGAACGTAAGGCCCATAACCCACGCCATAATGACCAAGCGTACTACCGAAGCGGTGAGGTTCAGTCCGTTGCAGTTTCGGGTTCGCCATCCCTGCAACATCAATAAAACCGCCCATCCCCAAAATGGTGTTAAGGAAAAAGCGGGTAAAGTgg > 1:750997/1‑238 (MQ=255) gTCAGCCAGGAAAGAACCGGGTAAAAACCATCCGCCATATCACCACCGTCATCACGCAGCGTGAAGCTACCGTAGAACGGTAACTGAACGTAAGGCCCATAACCCACGCCATAATGACCAAGCGTACTACCGAAGCGGTGAGGTTCAGTCCGTTGCAGTTTCGGGTTCGCCATCCCTGCAACATCAATAAAACCGCCCATCCCCAAAATGGTGTTCAGGAAAAAGCGGGTAAAGTGGa > 2:388905/1‑238 (MQ=255) cacccAGGAAAGAACGGGGAAAAACCCACCCGCCATATCACCACCGTCATCACGCAGCGTGAAGCTACCGTAGAACGGTAACTGAACGTAAGGCCCATACCCCACGCCATAATGACCAAGCGTACTACCGAAGCGGTGAGGTTCAGTCCGTTGCAGTTTCGGGTTCGCCATCCCTGCAACATCAATAAAACCGCCCATCCCCAAAATGGTGTTCAGGAAAAAGCGGGTAAAGTGGAcc < 2:1202943/235‑1 (MQ=255) cAGCCAGAAAAGAACCGGGTAAAAACCATCCGCCATATCACCACCGTCATCACGCAGCGTGAAGCTACCGTAGAACGGTAACTGAACGTAAGGCCCATACCCCACGCCATAATGCCCAAGCGTACTACCGAAGCGGTGAGGTTCAGTCCGTTGCAGTTTCGGGTTCGCCATCCCTGCAACATCAATAAAACCGCCCATCCCCAAAATGGTGTTCAGGAAAAAGCGGGTAAAGTGGAcc < 1:72275/238‑1 (MQ=255) gCCAGGAAAGAACCGGTTAAAAACCATCCGCCATATCACCACCGTCATCACGCAGCGTGAAGCTACCGTAGAACGGTAACTGAACGTAAGGCCCATAACCCACGCCATAATGACCAAGCGTACTACCGAAGCGGTGAGGTTCAGTCCGTTGCAGTTTCGGGTTCGCCATCCCTGCAACATCAATAAAACCGCCCATCCCCAAAATGGTGTTCAGGAAAAAGCGGGTAAAGTGGACCATc < 1:516227/239‑1 (MQ=255) cataaaaGAGCCGGCAAAAACCCTACCGCCAGTTCACCACCTTCATCCCGCAGCGTGAAGCTACCGTAGAACGGTAACTGAACGTAAGGCCCATAACCCACGCCATAATGTCCAAGCGTACTACCGAAGCGGTAAGGTTCAGTCCGTTGCAGTTTCGGGTTCGCCATCCCTGCAACATCAATAAAACCGCCCATCCCCAAAATGGTGTTCAGGAAAAAGCGGGTAAAGTGGACCATcc < 2:105126/234‑1 (MQ=255) caagAAAGAACCGGGAAAAAACCATCCGCCAGATCACCACCGTCATCACGCAGCGTGAAGCTACCGTAGAACGGTAACTGAACGTAAGGCCCATACCCCACGCCATAATGACCAAGCGTACTACCGAAGCGGTGAGGTTCAGTCCGTTGCAGTTTCGGGTTCGCCATCCCTGCAACATCAATAAAACCGCCCATCCCCAAAATGGTGTTCAGGAAAAAGCGGGTAAAGTGGACCATccc < 2:584805/236‑1 (MQ=255) cAGTAAAGACCCGGGAAAAACCCATCCGCCATATCCCCACCGTCATCACGCAGCGTGAAGCTACCGTAGAACGGTAACTGAACGTAAGGCCCATAACCCACGCCATAATGACCAAGCGTACTACCGAAGCGGTGAGGTTCAGTCCGTTGCAGTTTCGGGTTCGCCATCCCTGCAACATCAATAAAACCGCCCATCCCCAAAATGGTGTTCAGGAAAAAGCGGGTAAAGTGGACCATccc < 2:1138031/239‑1 (MQ=255) cAGGAAGGACCCGGATAAAAACCACCCGCCAAATCACCACCGTCATCACGCAGCGTGAAGCTACCGTAGAACGGTAACTGACCGTAAGGCCCATAACCCACGCCATAATGACCAAGCGTACTACCGAAGCGGTGAGGTTCAGTCCGTTGCAGTTTCGGGTTCGCCATCCCTGCAACATCAATAAAACCGCCCATCCCCAAAATGGTGTTCAGGAAAAAGCGGGTAAAGTGGACCATccc < 1:1054758/239‑1 (MQ=255) cAGGAAAGAACCGGGTAAAACCCATCCGCCATATCACCACCGTCATCACGCAGCGTGAAGCTACCGTAGAACGGTAACTGAACGTAAGGCCCATAACCCACGCCATAATGACCAAGCGTACTACCGAAGCGGTGAGGTTCAGTCCGTTGCAGTTTCGGGTTCGCCATCCCTGCAACATCAATAAAACCGCCCATCCCCAAAATGGTGTTCAGGAAAAAGCGGGTAAAGTGGACCATccc < 1:375959/239‑1 (MQ=255) cAGGAAAGAACCGGGTAAAAACCATCCGCCATATCACCACCGTCATCACGCAGCGTGAAGCTACCGTAGAACGGTAACTGAACGTAAGGCCCATAACCCACGCCATAATGACCAAGCGTACTACCGAAGCGGTGAGGTTCAGTCCGTTGCAGTTTCGGGTTCGCCATCCCTGCAACATCAATAAAACCGCCCATCCCCAAAATGGTGTTCAGGAAAAAGCGGGTAAAGTGGACCATcc < 1:606640/238‑1 (MQ=255) cAGAAAAAAACCGGGAAAAAACCAACCGCCATAACACCACCGTCATCACGCAGCTTGAAGCTACCGTAGAACGGTAACGGAACGTAAGGCCCATAACCCACGCCATAATGACCAAGCGTACTACCGAAGCGGTGAGGTTCAGTCCCTTGCAGTTTCGGGTTCGCCATCCCTGCAACATCAATAAAACCGCCCATCCCCAAAATGGTGTTCAGGAAAAAGCGGGTAAAGTGGACCATccc < 1:538462/239‑1 (MQ=255) aaaGAACCGGGTAAAAACCATCCGCCATATCACCACCGTCATCACGCAGCGTGAAGCTACCGTAGAACGGTAACTGAACGTAAGGCCCATAACCCACGCCATAATGACCAAGCGTACTACCGAAGCGGTGAGGTTCAGTCCGTTGCAGTTTCGGGTTCGCCATCCCTGCAACATCAATAAAACCGCCCATCCCCAAAATGGTGTTCAGGaaaa > 2:41995/1‑213 (MQ=255) aaaGAACCGGGTAAAAACCATCCGCCATATCACCACCGTCATCACGCAGCGTGAAGCTACCGTAGAACGGTAACTGAACGTAAGGCCCATAACCCACGCCATAATGACCAAGCGTACTACCGAAGCGGTGAGGTTCAGTCCGTTGCAGTTTCGGGTTCGCCATCCCTGCAACATCAATAAAACCGCCCATCCCCAAAATGGTGTTCAGGaaaa < 1:41995/213‑1 (MQ=255) aaGAACCGGGTAAAAACCATCCGCCATATCACCACCGTCATCACGCAGCGTGAAGCTACCGTAGAACGGTAACTGAACGTAAGGCCCATAACCCACGCCATAATGACCAAGCGTACTACCGAAGCGGTGAGGTTCAGTCCGTTGCAGTTTCGGGTTCGCCATCCCTGCAACATCAATAAAACCGCCCATCCCCAAAATGGTGTTCAGGAAAAAGCGGGTAAAGTGGACCATCCCCTGAt < 1:388905/239‑1 (MQ=255) ggTAAAAACCATCCGCCATATCACCACCGTCATCACGCAGCGTGAAGCTACCGTAGAACGGTAACTGAACGTAAGGCCCATAACCCACGCCATAATGACCAAGCg > 1:92236/1‑105 (MQ=255) ggTAAAAACCATCCGCCATATCACCACCGTCATCACGCAGCGTGAAGCTACCGTAGAACGGTAACTGAACGTAAGGCCCATAACCCACGCCATAATGACCAAGCg < 2:92236/105‑1 (MQ=255) | TTAAATCATCCTGAATCGCTTGTGCGTTCGGATTTTCCTGCGGTTTGAGTTCGCCGCCATTAGCGATGAAATCATGACGCTGGAAGTACGCTTCGCGCACCATAATAT‑AAGGATCGGACGACTGACGCAGCAGACCATCGGAATCCAGCAGCTGAGCGCGGGTTTCGATCCCTTCAAGCGTCCATTTACCCACAGACATCGGCCAGGTCAGCCAGGAAAGAACCGGGTAAAAACCATCCGCCATATCACCACCGTCATCACGCAGCGTGAAGCTACCGTAGAACGGTAACTGAACGTAAGGCCCATAACCCACGCCATAATGACCAAGCGTACTACCGAAGCGGTGAGGTTCAGTCCGTTGCAGTTTCGGGTTCGCCATCCCTGCAACATCAATAAAACCGCCCATCCCCAAAATGGTGTTCAGGAAAAAGCGGGTAAAGTGGACCATCCCCTGAT > NC_000913/2464272‑2464727 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |