Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	3,206,506	C→A	25.2%	T15K (ACA→AAA)	ttdA →	L‑tartrate dehydratase, alpha subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,206,506	0	C	A	25.2%	104.1 / 9.8	66	T15K (ACA→AAA)	ttdA	L‑tartrate dehydratase, alpha subunit
Reads supporting (aligned to +/- strand):  ref base C (16/31);  new base A (6/11);  total (24/42)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAAC‑TGTTAATTACATATTCACGGTGGCAAAAAAT‑ATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAA‑TTTTTCCAGACAAATAC‑AAAAACT‑G‑GA‑GTTGCCA‑TGATGAGCG‑AAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTAC‑CGCCATGATTTCT‑ACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCG‑TCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCTTCCCACTGCTTGGCG  >  NC_000913/3206268‑3206736                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                          cGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAAC‑TGTTAATTACATATTCACGGTGGCAAAAAAT‑ATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAA‑ATTTTTTATAAAAATAA‑AAAAAAT‑G‑GA‑GGTGTCA‑TGGTGAGGG‑GTAGTAATAAACAACAAGCAATGAACAAGTGGAc                                                                                                                                                                                                                                           >  2:42500/1‑239 (MQ=255)  gCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAAC‑TGTTAATTACATATTCACGGTGGCAAAAAAT‑ATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAA‑TATTTTCAGACAAATAC‑AAAAAAT‑G‑GG‑GTTTCCA‑GGGTTGGCG‑AAAGATAAAAGCAAAACGCAGTGAATAAATTGGCa                                                                                                                                                                                                                                          >  1:766827/1‑239 (MQ=255)       tgtgATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAAC‑TGTTAATTACATATTCACGGTGGCAAAAAAT‑ATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAA‑TTTTTCCAGACAAATAC‑AAAAACT‑G‑GA‑GTTGCCA‑TGATGAGCG‑AAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAgaga                                                                                                                                                                                                                                      <  2:560479/238‑1 (MQ=255)       tgtgATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAAC‑TGTTAATTACATATTCACGGTGGCAAAAAAT‑ATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAA‑TTTTTCCAGACAAATAC‑AAAAACT‑G‑GA‑GTTGCCA‑TGATGAGCG‑AAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAgaga                                                                                                                                                                                                                                      <  1:849748/238‑1 (MQ=255)       tgtgATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAAC‑TGTTAATTACATATTCACGGTGGCAAAAAAT‑ATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAA‑TTTTTCCAGACAAATAC‑AAAAACT‑G‑GA‑GTTGCCA‑TGATGAGCG‑AAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGAt                                                                                                                                                                                                                                     <  1:74784/239‑1 (MQ=255)       tgtgATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAAC‑TGTTAATTACATATTCACGGTGGCAAAAAAT‑ATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAA‑TTTTTCCAGACAAATAC‑AAAAACT‑G‑GA‑GTTGCCA‑TGATGAGCG‑AAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGAt                                                                                                                                                                                                                                     <  1:457775/239‑1 (MQ=255)           aTAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAAC‑TGTTAATTACATATTCACGGTGGCAAAAAAT‑ATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGGA‑ATTTTTCACACAAAAAC‑AAAAAAT‑T‑GA‑GTTGCCA‑TGATGAGAG‑GAAATAAAAAAAAAAAAGAAATGAATAAGTTTAAAAAAATTGTc                                                                                                                                                                                                                                 >  2:757226/1‑239 (MQ=255)           aTAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAAC‑TGTTAATTACATATTCACGGTGGCAAAAAAT‑ATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAA‑TTTTTTTATACAAAAAA‑AAAAAAT‑G‑AC‑GTTGTCC‑TTAGCAGGGCAAAGT‑ATAAAAAAAAAGCAATGAAAAAGTGTACAGAGATTg                                                                                                                                                                                                                                   >  2:13286/1‑237 (MQ=255)              aGCTCAGTTTATTTTGAAAAACATTGCCATTCCCTGCTCTGTCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAAT‑TGTTAATTACATATTCACGGTGGCAAAAAAT‑AAAAAACCACAATTTTAGTGGTATTTTGTGGCGG‑TAAA‑TTTTTCAAAACAAATAC‑AAAAACT‑G‑GA‑GTTGCCA‑TGATGAGCG‑AAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCt                                                                                                                                                                                                                              <  2:609553/239‑1 (MQ=255)              aGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAAC‑TGTTAATTACATATTCACGGTGGCAAAAAAT‑ATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAA‑TTTTTCCAGACAAATAC‑AAAAACT‑G‑GA‑GTTGCCA‑TGATGAGCG‑AAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCt                                                                                                                                                                                                                              <  1:13286/239‑1 (MQ=255)                     tgtATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAAC‑TGTTAATTACATATTCACGGTGGCAAAAAAT‑ATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTTGAT‑TTTTTCCAG‑CAAAAACAAAAAATT‑G‑AA‑ATTTCCA‑‑GATTGGGG‑GAAGTAATAAAGAACAAGCAAGGAAAAAATTGACAAAGAATTTTGCTAACattt                                                                                                                                                                                                                       >  1:1045251/1‑238 (MQ=255)                     tgtATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAAC‑TGTTAATTACATATTCACGGTGGCAAAAAAT‑ATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTTGAA‑TTTTT‑AAAAAAAAAAC‑AAAAAAT‑GTGA‑TTTGCCAGAGATGAGAG‑‑AGGTAA‑AATACATAAGAAGATAATAAGCTGTCAGAGATTCTTGCTAACttt                                                                                                                                                                                                                        >  2:1124421/1‑238 (MQ=255)                           tcccAAAACTTCGCTATTCCCGGCTTTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAAC‑TGTTAATTACATTTCCGCGGTGGCAAAAAAT‑ATAAAACCACATTTTTAGTGGTATTTTGGGGGGG‑TGAA‑TTTTCCCAGACAAATAC‑AAAAACT‑G‑GA‑GTTGCCA‑TGATGAGCG‑AAAGTAATAAGCACCAGGCAGTGAAAAAGTTGACAGAGATTGTCGCTAACTTTAC‑CGCCa                                                                                                                                                                                                                <  1:909580/236‑1 (MQ=255)                             acAAAACATTGCCATTCCCTGCTCTGGCGAAATATTAATTCTTATTTTCTCTTTGATTCACGAAC‑TGTTAATTACATATTCACGGTGGCAAAAAAT‑AAAAAACCACATTTTTAGTGGTAGTTTGGGGCGG‑TGAA‑TTTTTCCAGACAAATAC‑AAAAACG‑G‑GA‑TTTGCCA‑TGATGAGCG‑AAAGTAATAACCAACAGGCAGTGAATAAGTTGAAAGAGATTGTCGCTACTTTTAC‑CGCCATg                                                                                                                                                                                                              <  1:391680/238‑1 (MQ=255)                               aaaaCATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAAC‑TGTTAATTACATATTCACGGTGGCAAAAAAT‑ATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAA‑TTTTTTCACACAAAAAA‑TAAAAAT‑T‑GA‑GTTTTGC‑TTATTAGCG‑TAAATAATAAAAAATACGAAGAGAATAAATTAGAACAGAGTGTCGCTCACCTTAT‑ACCCATGAt                                                                                                                                                                                                            >  1:854016/1‑239 (MQ=255)                                 aaCATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAAC‑TGTTAATTACATATTCACGGTGGCAAAAAAT‑ATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAATTTTTTCAAGACAAA‑AC‑AAAAAAT‑A‑CA‑GTTGCCA‑TCATGGGCG‑GAAGGAATAAGACACAAGCAGTGACAAAGTTGACAAAGATTATTGATAACTTTtc‑ctcccttcttt                                                                                                                                                                                                          >  2:915883/1‑232 (MQ=255)                                      tGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAAC‑TGTTAATTACATATTCACGGTGGCAAAAAAT‑ATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAA‑TTTTTTCAGACAAATAC‑AAAAAAT‑G‑TA‑GGTGCCA‑TGATTAGCG‑AAAGTAATAAAAAACAGGGAGGGAATAAGTTTACAGAGAGTGTGGCTAAATTTAC‑CCCCATGATTTCT‑Ac                                                                                                                                                                                                     >  1:1101461/1‑238 (MQ=255)                                         taTTCCCTTGTTTGCCGAAATATTCATTCTTATTTTCGCTTTGATTCACGAAA‑TGTTAATTACATATTCACGGTGGCAAAAAAT‑AAAAAACCAAATTTTTAGTGGTAGTTTGGGGCGG‑TGAA‑TTTTCCCAAACAAATAA‑AAAAACT‑G‑GA‑GTCGCCA‑TGATGAGCG‑AAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTAC‑CGCCATGATTTCT‑ACCCGa                                                                                                                                                                                                 <  1:847011/238‑1 (MQ=255)                                          aTTCCCTTCTCGGGCGAAATATTCATTCTTATTTTCGCTTTGATTCACGAAC‑TGTTAATTACATATTCACGGTGGCAAAAAAT‑AAAAAACCACATTTTTAGTGGTAGTTTGTGGGGG‑TGAA‑TTTTTCCAGACAAATAC‑AAAAACT‑G‑GA‑GTTGCCA‑TGATGAGCG‑AAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTAC‑CGCCATGATTTCT‑ACCCGaa                                                                                                                                                                                                <  1:829109/239‑1 (MQ=255)                                                   aaaaagcaaaTATTCATTCTAATTTCGGCGTTAATTC‑CAAACTTGTTAATTACAATTTGAGGGTGGAAAAAAAT‑AAAAAACAAAATTTTAAGTGGTAGTTGGTGGGGG‑TGAA‑TTTTTCAAAAAAAAAAA‑AAAAACG‑G‑GA‑GTGGCCA‑TGAGGAGCG‑AAAGTAATAAGCAACAGGTAGTGAATAAGTTGACGGAGATTGTCGTTACCTTTAC‑CGCCATGATTTCT‑ACCCGAATGCCtgatg                                                                                                                                                                                       <  2:339379/231‑1 (MQ=255)                                                             tataCATTCTTATTTTCGCGTTAATTCACGACC‑TGTTAATTACATATTCACGGTGGAAAAAAAT‑ATAAAACCCCATTTTTAGTGGTAGTTTGTGGCGG‑TGAA‑TTTTACCAGAAAAATAC‑AAAAACT‑G‑GA‑GTTGCAA‑TGAGGAGCG‑AAAGTAATAAGCAACAGGCAGTGAAAAAGTTGACAGAGATTGTCGCTAATTTTAC‑CGCCATGATTTCT‑ACCCGAATGCCTGATGACGTGGTGGa                                                                                                                                                                             <  1:166732/239‑1 (MQ=255)                                                             aattcactcTTATTTTCGGTTTGATACACAAAG‑TGTTAATTACATATGCACGGTGGAAAAAAAT‑AAAAAACAAAATTTTGAGGGGTAGTTTGTGGGGT‑AGAA‑TTTTTCCAGAAAAAAAA‑AAAAACT‑G‑GA‑GTTGCCA‑TGATGAGCA‑AAAGTAATAAGCAACGGGCAATAAATAAGTTGACAGATATTGTCGCTAATTTTAC‑CGCCATGATTTCT‑ACCCGAATGCCTGATGACGTGGTGGa                                                                                                                                                                             <  2:125645/238‑1 (MQ=255)                                                                     cTTATTTTCGTGTTTATTCACGAAC‑TGTAAATTAAATATTCACGGTGAGAAAAAAA‑ATAAAACCAAATTTTGAGGGGGAGTTTGTGGCGG‑TGAA‑TTTTTCAAGAAAAAAAC‑AAAAACT‑G‑GT‑GTTGCCA‑TGATGAGCG‑AAATTAATAAGCAACAGGGAGTGAATAAGTTGAAAGAGATTGTCGATAACTTAAC‑CGCCATGATTTCT‑ACCCGAATGCCTGATGACGTGGTGGATAAACTaa                                                                                                                                                                     <  1:608541/239‑1 (MQ=255)                                                                     cTTATTTACGGATTGATCCATGATC‑TGTTAATTAAATATTAAGGGTGAAAAAAAAT‑AAAAAACCACATTTTAAGTGGAATTTAGTGGCGG‑TGAA‑TTTTACCAGAAAAAAAA‑AAAACCG‑G‑GA‑TTTGCAA‑TGATGAGCG‑AAAGTAATAACCAACAGGTAGTTAAAAAGTGGAAAGAGATTGTCGTTACTTTTAC‑CGCCATGATTTCT‑ACCCGAATGCCTGATGACGTGGTGGATAAACTaa                                                                                                                                                                     <  2:529126/239‑1 (MQ=255)                                                                     cTTATTTACGCTTTAATTCACGAAT‑TGTTAATTACATATGCACGGTGGCAAAAAAT‑AAAAAACCACATTTTTAGGGGTAGTTTGTGGCGG‑TAAA‑TTTTCCCAGACAAATAC‑AAAAACT‑G‑GA‑GTTGCCA‑TAATGAGCG‑AAAGTAAAAAGCAACAGGCAGTGAATAATTTGACAGAGATTGTCGCTAACTTTAC‑CGCCATGATTTCT‑ACCCGAATGCCTGATGACGTGGTGGATAAACTaa                                                                                                                                                                     <  1:148751/239‑1 (MQ=255)                                                                      ttATTTTCGTGTTAATCCACAAAC‑TGTTAATTACATTTTCACGGTGGCAAAAAAT‑AAAAAACAACATTTATAGGGGTGGTTTGTGGCGG‑TAAA‑TTTTCCAAAAAAAATAC‑AAAAACG‑G‑GA‑GTTCCAA‑TAAGGATCA‑AAATAAATAAGCAACAGGCATTAAATAAGTGGAAAGAGATTGTCGTTAACTTTAC‑CGCCATGATTTCT‑ACCCGAATGCCTGATGACGTGGTGGATAAACTaaa                                                                                                                                                                    <  2:161297/239‑1 (MQ=255)                                                                       tATTTTCGCGTTGATTCACGAAC‑TTTTAATTACATATCCCCGGGGGAAAAAAAT‑AAAAACCCACATTTTTAGTGGTAGTTTGTGGCGG‑TAAA‑TTTTTCAAGACAAATAC‑AAAAACT‑G‑GA‑GTTGCCA‑TGATGAGCA‑AAAGTAATAAGCAACAGGCAGTAAATAAGTTGACAGAGATTGTCGCTAACTTTAC‑CGCCATGATTTCT‑ACCCGAATGCCTGATGACGTGGTGGATAAACTaaaa                                                                                                                                                                   <  1:42500/239‑1 (MQ=255)                                                                       tATTTTCGCGTTGATTCACGAAC‑TGTTAATTACATATTCACGGTGGCAAAAAAT‑ATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAA‑ATTTTTTATACAAAAAC‑AAAAACT‑T‑GA‑GTTGCCA‑TTGTGTGGG‑TAACGAAAAAAAAAAAAGCACAGAAAAAATTTGAAAAGATTTTGCATAACTTTTA‑CGCCCATATTTCT‑ACCCGAATGCCTCGTGAAGCGGTGGGTAAACTaaa                                                                                                                                                                    >  2:702996/1‑238 (MQ=255)                                                                       tATTTTCGCGTTGATTCAAGAAC‑TGTTAATTACATATTCACGGTGGCAAAAAAT‑AAAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAA‑TTTTTCAAGACAAATAC‑AAAAACG‑G‑GA‑GTTGCCA‑TGATGAGCG‑AAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTAC‑CGCCATGATTTCT‑ACCCGAATGCCTGATGACGTGGTGGATAAACTaaaa                                                                                                                                                                   <  1:154220/239‑1 (MQ=255)                                                                                                gTTAATTACATATTCACGGTGGCAAAAAAT‑ATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAA‑ATTTTTCAAACAAAAAC‑AAAAAAA‑G‑GA‑GTTTGCA‑TTGTGGGGG‑AAAGGAAAAAAAAAAAAGGAAGGAAAAAATTGACAGAGATTTTCGCTAACCTTAA‑CCCCAAGATTTTT‑TACCGAATGACTGGAGACGGGGGGGGTAAAAAAAAAAAACTTAAAGAACCCGaaaaggcc                                                                                                                                           >  2:25389/1‑234 (MQ=255)                                                                                                    attaattatTCAGGGGGAAAAAAAAT‑ATAAAACAACATTTATAGTGGTAGTTTGTGGGGG‑TAAA‑TTTTACCAGACAAATAC‑AAAAACG‑G‑AA‑GTTGCCA‑TGATGAGGA‑AAAGTAATAAGCAACAGGCAGTTAATAAGTTGACTGAGATGGTCGCTAACTTTAC‑CGCCATGATTTCT‑ACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCG‑TCGa                                                                                                                                      <  1:344051/233‑1 (MQ=255)                                                                                                     ttAAATATCCAC‑GTTGCAAAAAATAATAAAACCACTGTATTAGTGGTAGTTTGTGTCGG‑TGAA‑TTATGCCAGAAAAATAC‑AAAAACG‑G‑GA‑GTTTCAA‑GGATGAGCA‑AAATTAATAAGCAACAGGAAGGTAATAGGTTGACAGAGATTGTCGATATTTTTAC‑CGCCATGATTTCT‑ACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCG‑TCGAt                                                                                                                                     <  2:322415/239‑1 (MQ=255)                                                                                                          tgttcggggTGGCAAAAAAT‑AAAAAACCAAATTGTTAGTGGTAGTTTGTGGGGG‑TGAA‑TTTTCCCAAAAAAAAAC‑AAAAACT‑G‑GT‑GTTGCCA‑GAATGAGGA‑AAATTAAAAAGCAACGGGCAGTGAATAGGTGGACAGAGATTGTCGTTAACTTTAC‑CGCCATGATTTCT‑ACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCG‑TCGATGGGGa                                                                                                                                <  2:1045251/232‑1 (MQ=255)                                                                                                          tatTCAGGGTGGAAAAAAAT‑AAAAAACCACATTTTTAGTGGTAGTTTGGGGCGG‑TAAT‑TTTTTCCAGACAATTAA‑AAAAACA‑G‑GA‑GTGGCCA‑TGAGGAGGA‑AAAGTAAAAAGAAACAGGCGGGGAAGAAATGTAAATAGGTGGCCGGAAGCTTCAC‑CGCCATGATTTCT‑ACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCG‑TCGATGGGGa                                                                                                                                <  1:109395/239‑1 (MQ=255)                                                                                                           atTCACGGTGGCAAAAAAT‑ATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAA‑TTTTTTCAAACAAAAAA‑AAAAAAT‑T‑GA‑GTTTCCA‑TGATGAGCG‑GAAAGAATAAACAAAAAGCAGTGAAAAAGATTACAGAGATTGTCGCTACATTTAC‑CCCCATGATTTCT‑ACCCGAAAGCCTGATGACGTGGTGGAGAAACTAAAAAAACTAAAAGAAGCCGGAACATCG‑TCGATggggga                                                                                                                               >  1:958632/1‑237 (MQ=255)                                                                                                           atTCACGGTGGCAAAAAAT‑ATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAA‑TTTTTCCAGACAAATAC‑AAAAAAT‑T‑GA‑GTTGCCA‑TGATGAGCG‑CAAGTAATAAACAACAAGCAGGGGAAAAAATGACAGAGAGTTTCGCTAAATTTAC‑CGCCATGATTTTT‑ACCCGAATACCTGATGAAGGGGGGGGTAAACTAAAAAAACTAAAGGATGCCGAAAACACG‑TCGGTGGGGaa                                                                                                                               >  1:1101168/1‑239 (MQ=255)                                                                                                           atTCACGGTGGCAAAAAAT‑ATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAA‑ATTTTTCTGACAAATAA‑AAAAAAT‑G‑GG‑GGTGTCA‑TTGAGGGCG‑GAAGGTAAAAACAAAAAGAAAAGAATAAATTTATAGAGAACGTCGCTAACTTTACACCCCAGATTTTTT‑ACCCAAATG‑CTGAGGCAGGGGTGGGTAAAATAAAAAAAATTAAAGATATCCAAAAGTCG‑TCGAtatggga                                                                                                                               >  2:726296/1‑233 (MQ=255)                                                                                                           atTCACGGTGGCAAAAAAT‑ATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAA‑ATTTTTCAAACAAAAAC‑AAAAAAT‑G‑GA‑GTTGGCA‑TGGTGAGCG‑AAAGTAATAAACAAAAAGCAGAGAAAAAAATGATAGAGATTGGCTGTAAATTTAC‑CACCACGATTTTT‑ACCCGAAGACCAGATGACGCGGGGGATAAACAAAAAAAAACAAAAAATTCCGACAcgtcg‑tacaggggggg                                                                                                                               >  2:966271/1‑229 (MQ=255)                                                                                                               aCGGTGGAAAAAAAT‑ATAAAACCACATTTTTAGTGGTAGTTGGTGGCGG‑TGAA‑TTTTCCCAGACAAATAC‑AAAAACT‑G‑GA‑GTTGCCA‑TGATGAGCA‑AAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTAC‑CGCCATGATTTCT‑ACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCG‑TCGATGGGGAAAAtt                                                                                                                           <  1:757226/239‑1 (MQ=255)                                                                                                               aCGGTGGAAAAAAAT‑AAAAACCCAAATTTTTAGTGGTAGTTTGTGGCGG‑TGAA‑TTTTTCAAAAAAAATAA‑AAAAACT‑G‑GA‑GTTGCCA‑TGATGAGCA‑AAAGTAATAAGAAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTAC‑CGCCATGATTTCT‑ACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCG‑TCGATGGGGAAAAtt                                                                                                                           <  1:470818/239‑1 (MQ=255)                                                                                                                 ggtgaaaaaaaaT‑AAAAAACCACATTTTTAGTGGTAGTTGGGGGCGG‑TGAA‑TTTTCCCAGACAAATAC‑AAAAACT‑G‑GA‑GTTGCCA‑TGATGAGCG‑AAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTAC‑CGCCATGATTTCT‑ACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCG‑TCGATGGGGAAAATTAt                                                                                                                         <  1:25389/233‑1 (MQ=255)                                                                                                                  ggggCAAAAAAT‑ATAAAACCATATTTTTGGGGGTAGGTTGGGGCGG‑TGAA‑TTTTTCCAGACAAATAC‑AAAAACG‑G‑GA‑GTTGCCA‑TGAGGAGGG‑AAAGTAATACGCAAAGGGTAGTGAATAGGTTGACAGAGATTGTCGTTACCTTTAC‑CGCCATGATTTCT‑ACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCG‑TCGATGGGGAAAATTAt                                                                                                                         <  1:164518/236‑1 (MQ=255)                                                                                                                   tGGCAAAAAAT‑ATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAA‑TTTTTTCAAACAAAAAA‑AAAAAAT‑T‑GAGGTTGCCT‑TGGTGGGGG‑AAATAAAAAAAAAACA‑GCAAAGAATAAGGTGAACAAGATTTTCCCTTAATTTAA‑CACCAATATTTTTAACCCGATGCCCTGTTACGGTGTGGGAAAAACAAAAAAAGTCAAAGAAACCCAAAACGCCG‑GCG‑TGGGGGAAATTAttt                                                                                                                       >  2:285026/1‑237 (MQ=255)                                                                                                                         aaaaT‑ATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAA‑TTTTTCCAGACAAATAC‑AAAAACT‑G‑GA‑GTTGCCA‑GGATGAGCA‑AAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCAAATTTTAC‑CGCCATGATTTCT‑ACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCG‑TCGATGGGGAAAATTATCTACCATa                                                                                                                 <  1:1124421/239‑1 (MQ=255)                                                                                                                           aaT‑ATAAAACCACATTTTTGGTGGTAGTTGGGGGGGG‑TGAA‑TTTTTCAAAAAAAATAC‑AAAAACT‑G‑GA‑GTTGCCA‑TGAGGAGCA‑AAAGTAATAAGCAACAGGCAGGGAATAAGTTGAAAGAGATTGTCGCTAACTTTAC‑CGCCATGATTTCT‑ACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCG‑TCGATGGGGAAAATTATCTACCATACg                                                                                                               <  1:915883/239‑1 (MQ=255)                                                                                                                                  aaaCCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAA‑ATTTTTCAAACAAAAAA‑AAAAAAA‑T‑GA‑GGTGGCA‑TGATGGGGG‑AAAGGAATAAAAAACAAGCAAAAAAAAAAATGAAAGAGAATGTCGCCAACTTTAC‑ACCCATGATTTCT‑ACCCCAAAAACTGATGACGGGGGGGATAAACTAAAAAAGTCAAACAGAGCCGCAACATCG‑TCGGTGGGGGAAATTATATTCCCCACGAAGTTc                                                                                                         >  2:350200/1‑239 (MQ=255)                                                                                                                                        caTTTTTAGTGGTAGTTTGTGGCGG‑TGAA‑TTTTTTCAGACAAATAC‑AAAAACT‑T‑GA‑GGTTTCA‑TGGTGCGAC‑ATAATAAAATACAAAAGACATTGAATGAGGTGTAAGAATTTCTAGCTTAAATTAC‑CGCCCTCAGTTTT‑ACCACCACGACTGATGACGTGGTGGATATACTCACACACCTTAATGCGGCCACAACATCGCTCGA‑GGGGGAAATTATAATCAATACCATGTTCTacaaa                                                                                                   >  2:12608/1‑238 (MQ=255)                                                                                                                                                  ggTAGTTTG‑GGGGG‑GGAA‑ATTTTTCAAAAAAAAAC‑AAAAACTGG‑GA‑TTTGCAA‑TGATGAGGA‑AAAGTAAAAAGAAACAGAAAGTGAATAGGTTAAAAGAGATTGCCGTTAATTTTAC‑CGCCATGATTTCT‑ACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCG‑TCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCaaaa                                                                                           <  2:83476/237‑1 (MQ=255)                                                                                                                                                      gtgtggggggg‑TAAA‑TTTTGCAAAAAAAAAAC‑AAAAAGT‑G‑AA‑GTTGCCA‑GGGTGAGCG‑AAAGTAATAAGCAAGAGGAAGTAAATAAGTGGAAGGAAATTGTCGTTAATTTTAC‑CGCCATGATTTCT‑ACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCG‑TCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGa                                                                                      <  2:353338/229‑1 (MQ=255)                                                                                                                                                                         ttcccAGACAAATAA‑AAAAACT‑G‑GA‑GTTGCCA‑TGGTGAGCG‑AAATTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTAC‑CGCCATGATTTCT‑ACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCG‑TCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTcctg                                                                    <  1:538631/236‑1 (MQ=255)                                                                                                                                                                                   aaTAG‑AAAAACA‑G‑AA‑GTTGCAA‑TGAGGAGCG‑AAATTAATAAGCAACAGGAAGTGAATAAGTAGACAGAGATTGTCGATAACTTTAC‑CGCCATGATTTCT‑ACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCG‑TCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGa                                                          <  1:884732/239‑1 (MQ=255)                                                                                                                                                                                      aC‑AAAAACG‑G‑GA‑TTTGCCA‑TGATGAGCG‑AAATTAATAACCACCAGGAAGTAAATAATTTGACAGAGATTGTCTCTAACTTTAC‑CGCCATGATTTCT‑ACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCG‑TCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGacac                                                       <  1:1054575/239‑1 (MQ=255)                                                                                                                                                                                           aaaCT‑G‑GA‑GTTGCCA‑TGATGAGCG‑AAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTAC‑CGCCATGATTTCT‑ACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCG‑TCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCggg                                                   >  2:55895/1‑239 (MQ=255)                                                                                                                                                                                           aaaCT‑G‑GA‑GTGGCGA‑TGATGAGCG‑AAAGTAATAAGCAAAAGGCAGTGAAAAAGTGAACAGAGATTGACGCTAACTTTAC‑CGCCATGATTTCT‑ACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCG‑TCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCggg                                                   <  2:401234/239‑1 (MQ=255)                                                                                                                                                                                            aaCT‑G‑GA‑GTTGCCA‑TGATGAGCG‑AAAGTAATAACCAACAGACAGTGAATAAGTTGACAGGTATTGTCTCTAACTTTAC‑CGCCATGATTTCT‑ACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCG‑TCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCgggg                                                  <  1:915853/239‑1 (MQ=255)                                                                                                                                                                                            aaCT‑G‑GA‑GTTGCCA‑TGATGAGCG‑AAAATAATAAGCAACAGGCAGGGAATAAGTTGACAGAGATTGTCCCTACCTTTAC‑CGCCATGATTTCT‑ACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCG‑TCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCgggg                                                  <  1:382661/239‑1 (MQ=255)                                                                                                                                                                                                               gatgaGCG‑AAAGTAATAAGCAACAGGCAGTGAATACGTTGACAGAGATTGTCGCTAACTTTAC‑CGCCATGATTTCT‑ACCCGAATGCCTGATGACGTGGTGGATAACCTAAAACAGCTAAAGGATGCCGAAACGTCG‑TCGCTGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACTTGAATCGTCCTGCCTGTCAGGACACCGGAGAGATTATGTTCTTCg                                   >  2:643429/1‑239 (MQ=255)                                                                                                                                                                                                                     cG‑AAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTAC‑CGCCATGATTTCT‑ACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCG‑TCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAg                             >  2:736753/1‑239 (MQ=255)                                                                                                                                                                                                                                     ccAGGCAGTGAATAAGTTGACAGAGAGTGTCGCTATCTTTAC‑CGACATGATTTCT‑ACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCG‑TCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCTTcc              <  1:458224/238‑1 (MQ=255)                                                                                                                                                                                                                                      cacgCAGTCAATAAGTTGACAGAGATTGTCGCTAACTTTAC‑CGCCATGATTTCT‑ACCCGAATGCCTGATGACGCGGCGGATAAACTAAAACAGCTAAAGGGTGCCGAAACGTCG‑TCGATGGGGAAAATTATCTACCATCGGATGTTCGACAACACGCAAAAAGCGATTGACCTGAATCACCCTGCCTGTCAGGACACAGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGATTacc             >  2:738786/4‑239 (MQ=255)                                                                                                                                                                                                                                      cAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTAC‑CGCCATGATTTCT‑ACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCG‑TCGATGGGGAAAATTATCTACCATACGATGTTCGACACCATGCAACAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGtt                                        >  1:475701/1‑212 (MQ=255)                                                                                                                                                                                                                                      cAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTAC‑CGCCATGATTTCT‑ACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCG‑TCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCTTccc             >  1:651527/1‑239 (MQ=255)                                                                                                                                                                                                                                      cAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTAC‑CGCCATGATTTCT‑ACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCG‑TCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGCTCCCGCTTccc             >  1:340625/1‑239 (MQ=255)                                                                                                                                                                                                                                       aGGTAGTAAATAAGTAGACAGATCTGGTCGATAACTATAC‑CGCCATGATTTCT‑ACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACATCTAAAGGATGCCGAAACGTCG‑TCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGTCCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCTTCCCa            <  2:1201146/239‑1 (MQ=255)                                                                                                                                                                                                                                       aGGCAGTGAATAAGTTGAAAGGGATGGTCGCTAATTTTAC‑CGCCATGATTTCT‑ACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCG‑TCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCTTCCCa            <  1:871326/239‑1 (MQ=255)                                                                                                                                                                                                                                                 tAATTTGAAAGATATTTTCGCTAACTTTAC‑CGCCATGATTTCT‑ACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCG‑TCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCTTCCCACTGCTTGGCg  <  1:726296/239‑1 (MQ=255)                                                                                                                                                                                                                                                 aaaGTGGAAAGAGTTGGTCGTTAATTTTAC‑CGCCATGATTTCT‑ACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCG‑TCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCTTCCCACTGCTTGGCg  <  1:12608/238‑1 (MQ=255)                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                          CGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAAC‑TGTTAATTACATATTCACGGTGGCAAAAAAT‑ATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAA‑TTTTTCCAGACAAATAC‑AAAAACT‑G‑GA‑GTTGCCA‑TGATGAGCG‑AAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTAC‑CGCCATGATTTCT‑ACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCG‑TCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCTTCCCACTGCTTGGCG  >  NC_000913/3206268‑3206736

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑