Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	3,499,694	G→T	15.3%	intergenic (+79/‑216)	yhfL → / → frlA	small lipoprotein/putative fructoselysine transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,499,694	0	G	T	15.3%	204.9 / 2.1	72	intergenic (+79/‑216)	yhfL/frlA	small lipoprotein/putative fructoselysine transporter
Reads supporting (aligned to +/- strand):  ref base G (22/37);  new base T (10/2);  total (33/39)
Fisher's exact test for biased strand distribution p-value = 4.45e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.12e-01

GCACTGGTAGGTGCAGTACTGGCTACGTTAACTGCATGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTGCGGTCCTACTGCACAGTAAGTCTCTGACAGAAATCGGCTAACA‑CCGGTTTAATTGCCCGACGCCCTCT‑GGATTTTCCGAGGGCGTA‑TTTGTTT‑CTATGT‑C‑CT‑TCTTGC‑CCCC‑GCCCGTAAAAATA‑TTTTCACTTTAAATTCAATTTGATAACTACA‑TCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTTCCTCCACATTACATAACATCATATGTTGTTATATTCATCATGCATTGTCATGTTACCTTTTAAATGACTGCAAACTCTCCCCTACAACGTATTGGACAAGAAAAAGGTATCGCTATGGGAAGCCAGGAACTCCAA  >  NC_000913/3499469‑3499930                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                    gCACTGGTAGGTGCAGTACTGGCTACGTTAACTGCATGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTTCGGGCCTACTGCACAGTAAATCTCTTACAGAAATCCGGTAACC‑CCGGTTTTATTGACCCACCCCCCCT‑TGGTTTTTCTAGGGGGTA‑TTTTGTT‑CTTTTT‑T‑CT‑TTTTTc‑cc                                                                                                                                                                                                                                     >  1:858327/1‑238 (MQ=255)            tGCAGTACTGGCTACGTTAACTGCATGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTTCGGTCCTACTGCACAGGAAATTTCTGACCCAAATAGGCTAACACCCGTTTTATTTCCCCCAGCCCCCCT‑GGTTTTTCGGGGGGGGAT‑TTTTTTT‑CT‑TGT‑C‑CT‑TCTTTC‑cccc‑cccccctaaa                                                                                                                                                                                                                        >  1:513069/1‑233 (MQ=255)               aGTACTGGCTACGTTAACTGCATGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGATGCCGTCCCACTCCGCCGTAAATCTCTTACAGAAATCGGCTTACA‑CCCGGTGTATTTCCCGACCCCCTCT‑GGATTTTTCGAGGGCGCA‑TTTGTTT‑TTATTT‑C‑CC‑TCTTTC‑CCCC‑CCCCCTGAACata                                                                                                                                                                                                                     >  2:676735/1‑239 (MQ=255)                 tACTGGCTACGTTAACTGCATGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTTGGGGCCCAATGACCACTAAGGCTCTGACCAAAAACTGATCACA‑CCGGGTTTATTGTCCGACGCCCTTTGGGATTTTAC‑AGGGCGGACTTTGTTT‑‑TATTT‑C‑GT‑TCTTTC‑CCCC‑CCCCTGAAAAATA‑tt                                                                                                                                                                                                                  >  1:1081316/1‑239 (MQ=255)                 tACTGGCTACGTTAACTGCATGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTTCGGTCCTCCTGCACCCCAAATTTTCTACACAAAACGGCTAACA‑CCCGTTTTATTTTCCTCCCCCCTCC‑GGTTTTTTGCACGGCGGA‑CTTGTTT‑CTTTGT‑T‑CT‑TCTTTT‑CCCC‑CCCCCTACAAataat                                                                                                                                                                                                                   >  2:704276/1‑237 (MQ=255)                 tACTGGCTACGTTAACTGCATGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTTCGGGCCCACTGCACAGTAAGTCTCTGAAAGAAATCGGCTAACA‑CCCGTTTAATTTCCCGACCCCCTCTGGGATTTTCCG‑GGGCGTA‑TTTTTTT‑CTATTT‑T‑CT‑TCTTTT‑CCCC‑CCCCGTCAAAATA‑tt                                                                                                                                                                                                                  >  1:1102114/1‑239 (MQ=255)                 tACTGGCTACGTTAACTGCATGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTTCCGGCCCCCCGCCCCGTATATTTCTGACAAAAATACGCGCACT‑CCGGGTTTTTTTTCCGCACCCCCCT‑GGATTTTTCGTAGGAGTG‑ATTGTTT‑TTGTATGC‑CT‑TTTTGC‑CCCC‑CCCCTTAAAAattt                                                                                                                                                                                                                    >  2:598568/1‑237 (MQ=255)                 tACTGGCTACGTTAACTGCATGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTGCGGTCCTACTGCACAGGAAATCTCTGAAAGAAAACGGCTAACA‑CCCGGTTTATTTTCCGACGCCCCCC‑TGATTTTCCGAGGGCGGA‑TTTGTTT‑CTATGT‑C‑CT‑TCTTTC‑CCCC‑CCCCGCCAAAAAA‑tt                                                                                                                                                                                                                  >  1:460045/1‑239 (MQ=255)                  aCTGGCTACGTTAACTGCATGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTTCGGTCCTACCTCACAATAAATCTCTTGCAGAAATAGGCTAAAA‑CCCGTTTATTTTTCCGACCCCCCCT‑GGATTTTCTCCGGGGGCC‑TTTTTTT‑CTATTT‑CTCTCTTTTCC‑CCCC‑CCCCGAAAAAattt                                                                                                                                                                                                                    >  1:512750/1‑237 (MQ=255)                      gCTCCGTTAACTGCATGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTGCGGTCCTACTGCACCGTAAGTCTCTGACAGAAATCGGCTCACC‑CCGGTTTACTTTCCCGACCCCCCCT‑TGGTTTTTCCAGGGCGGC‑TTTTTTT‑CTATGT‑C‑CT‑TCTTTC‑CCCCGGCCCGTAAAATtt‑tttt                                                                                                                                                                                                                >  1:430131/1‑237 (MQ=255)                      gCTACGTTAACTGCATGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTGCGGTCCTAATGCACAGTAAATCTCTGACAGAAATCGGCTAAAA‑CCGGTTTTATTGCCCGCCGCCCTCT‑TGATTTTCCGAGGGCGTA‑TTTGTTT‑CTTTGT‑T‑CT‑TTTTGC‑CCCC‑CTCCGGTAAAATA‑ttttt                                                                                                                                                                                                               >  1:518172/1‑236 (MQ=255)                         aCGTTAACTGCATGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTGCGGTCCTACTGCACAGTAAGTCTCTGACAGAAATCGGCTAACA‑CCGGTTTAATTGCCCGACGCCCTCT‑GGATTTTCCGAGGGCGTA‑TTTGTTT‑CTATGT‑C‑CT‑TCTTGC‑CCCC‑GCCCGTAAAAATA‑TTTTCACttt                                                                                                                                                                                                          <  1:907741/239‑1 (MQ=255)                           gTTAACTGCATGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGGTGCGGTCCCTCCGCCAAATAAATTTCTTACAACAATCGGGTAACACCCGGTTTATTTCCACGA‑GCCCTCT‑GGGCTTTCTGAGGGGGCC‑TTTTTTT‑CTTTTT‑C‑CC‑TTTTTC‑CCCC‑CCCCCTTAAAAAA‑tttttttttttt                                                                                                                                                                                                        >  2:407453/1‑231 (MQ=255)                               aCTGCATGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTTCGGGCCTACTGCCCACTAAGTCTCTGACACAAATTGGCCAACA‑CCCGTTTTATTTTCCGACCCCCCCT‑GGGGTTTTCCAGGGGGGT‑TTTTTTT‑TTTTTT‑T‑CT‑TCTTTCTCCCC‑CCCCTCAAAAATC‑TTTTcctttttattt                                                                                                                                                                                                     >  2:60123/1‑229 (MQ=255)                                    aTGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTTCGGGCCTACTGCACAGTAAATCTCTGACAGAAATTGGCTAACA‑CCGGGTTAATTGCCCGCCGCCCCCT‑GGATTTTCCGAGGGCGTT‑TTTGTTT‑CTATTT‑C‑CT‑TCTTGC‑CCCC‑GCCCCTAAAAATA‑TTTTTACTTTTAATTCAAttt                                                                                                                                                                                               >  1:1037896/1‑239 (MQ=255)                                           ggTCATCTTGCAAACCGTGATAAGAACTGCACTTACGCCTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCACTGCCGGTTGTGGGACTTAGGCACCCAAAATCTTTAACAAGAATCGGGCTACA‑GCGGGTTCATTACTCGATCCCCTCT‑TGATGTTTCTCGCGAGGA‑GTTGTTTCCTCTG‑‑C‑CT‑TCTTGC‑CCCC‑CCCCGTCAAACAA‑TTTTTTTTTTTTATTCGAGTTGATTACt                                                                                                                                                                                        >  2:777647/1‑239 (MQ=255)                                           ggTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTGCGGTCCTACTGCACCGAAAGTCTTTGAAAGAAAAACGCCAACA‑CCGGGTTAATTTCCCGCCGCCCCCT‑GGGTTTTCCTAGGGCGGA‑TTTGTTT‑CTATGT‑C‑CT‑TCTTGT‑CCCC‑GCCCGTAAAAATA‑TTTTCACTTTAAATTCAATTTTATaaat                                                                                                                                                                                        >  1:139137/1‑237 (MQ=255)                                              cATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTGCGGTCCTACTGCACAGTAAGTCTCTGACAGAAATCGGCTAACA‑CCGGTTTAATTGCCCGACGCCCTCT‑GGATTTTCCGAGGGCGTA‑TTTGTTT‑CTATGT‑C‑CT‑TCTTGC‑CCCC‑GCCCGTAAAAATA‑TTTTCACTTTAAATTCAATTTGATAACTAc                                                                                                                                                                                      <  1:830877/238‑1 (MQ=255)                                              cATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTGCGGTCCTACTGCACAGTAAGTCTCTGACAGAAATCGGCTAACA‑CCGGTTTAATTGCCCGACGCCCTCT‑GGATTTTCCGAGGGCGTA‑TTTGTTT‑CTATGT‑C‑CT‑TCTTGC‑CCCC‑GCCCGTAAAAATA‑TTTTCACTTTAAATTCAATTTGATAACTAc                                                                                                                                                                                      <  1:1056192/238‑1 (MQ=255)                                                       aaCCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAACTCATTGGCGGTTGTGGGTCTACCGCCCACTTAATCTCTCCCAAAAATTGGCTGACA‑AAGGGGTCATTGCCCGTCGACCTCT‑GGATTCTCCGAGGGCGGA‑TTTGTGT‑TTATGT‑T‑TT‑TCTTTG‑CCCC‑GCCCGCGAAAATA‑TGTTCACTTTTTATTCACTTTTCTTACTACaatctctat                                                                                                                                                                             >  2:461796/1‑230 (MQ=255)                                                               tAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTGCGGTCCTACTGCACAGTAAGTCTCTGACAGAAATCGGCTAACA‑CCGGTTTAATTGCCCGACGCCCTCT‑GGATTTTCCGAGGGCGTA‑TTTGTTT‑CTATGT‑C‑CT‑TCTTGC‑CCCC‑GCCCGTAAAAATA‑TTTTCACTTTAAATTCAATTTGATAACTACA‑TCCATTAGCCACAgcg                                                                                                                                                                    <  2:739724/238‑1 (MQ=255)                                                                        ctctTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTTGGGGCCCACTGCACAGTAAATCTCTGACAAGAATAGGCTAACA‑CCGGTTTAATTTCCCGACGCCCCCT‑GGGTTTTCCCAGGGCGGA‑TTTGTTT‑TTATGT‑C‑CT‑TCTTGC‑CCCC‑CCCCGTAAAAAAA‑TTTTCACTTTAAATTTAATTTTTTAATTACA‑ATCATTTACCACAGCCCGACCCtcgt                                                                                                                                                          >  2:923116/1‑236 (MQ=255)                                                                        ctctTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTTGCGTCCCTCCGCAAACTAAATTTCTTACATAAATAGCCTAACA‑CCCGTTTTTTTGCCCCACCCACCCT‑CGACTTTCCGTGGGCGTC‑ACTGTTT‑CTTTTT‑C‑CC‑TCTTGC‑CCCC‑CCCCGCACAAATC‑TTTTTCCTTTTACTTTCATTTTTTTACTACC‑ACCTCTAACCACAACGCCATCCtct                                                                                                                                                           >  2:589825/1‑236 (MQ=255)                                                                                   aCCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTGCGGTCCTACTGCACAGTAAGTCTCTGACAGAAATCGGCTAACA‑CCGGTTTAATTGCCCGACGCCCTCT‑GGATTTTCCGAGGGCGTA‑TTTGTTT‑CTATGT‑C‑CT‑TCTTGC‑CCCC‑GCCCGTAAAAATA‑TTTTCACTTTAAATTCAATTTGATAACTAc                                                                                                                                                                                      <  1:287498/201‑1 (MQ=255)                                                                                           cACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTTCGGGACCACAGCAAAATAAAACTCTGACAGAAATTAGCTAAAA‑ACGCGCTAATTTACCGCCACCCCCG‑GTATTTTTCGCGGAGGCA‑TTTTTTT‑TTATTT‑T‑GT‑TCTTTC‑CCCC‑CCCCCCAAAAATATTTTTCACTTTAATTTAAATTT‑ATAAATACATTCCTTTAACAACA‑CGCGATTCTGTTCGAGAtttttttgtttt                                                                                                                                         >  2:346388/1‑229 (MQ=255)                                                                                            aCCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTGCGGTCCTACTGCACAGTAAGTCTCTGACAGAAATCGGCTAACA‑CCGGTTTAATTGCCCGACGCCCTCT‑GGATTTTCCGAGGGCGTA‑TTTGTTT‑CTATGT‑C‑CT‑TCTTGC‑CCCC‑GCCCGTAAAAATA‑TTTTCACTTTAAATTCAATTTGATAACTACA‑TCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTtcct                                                                                                                                      <  1:891456/239‑1 (MQ=255)                                                                                                  catgttctatttctaAAAGCAGTGGCGGTTGCGGTCCTACTGGACAGTAAGTCTCTGACAGAGATCGGCTAACA‑CCGGTTTAATTGCCCGACGCCCTCT‑GGATTTTCCGAGGGCGTA‑TTTGTTT‑CTATGT‑C‑CT‑TCTTGC‑CCCC‑GCCCGTAAAAATA‑TTTTCACTTTAAATTCAAGTTGATAACTACA‑TCCATGAGCCACAGCTCGATCCTGTGCGAAATTTTGTGATCTTCCTCcaca                                                                                                                                 <  2:944223/234‑1 (MQ=255)                                                                                                  cAATTTCTATTTCTAAAATCATTGGCGGTTGCGGTCCTACTGCACAGTAAGTCTCTGACAGAAATCGGCTAACA‑CCGGTTTAATTGCCCGACGCCCTCT‑GGATTTTCCGAGGGCGTA‑TTTGTTT‑CTATGT‑C‑CT‑TCTTGC‑CCCC‑GCCCGTAAAAATA‑TTTTCACTTTAAATTCAATTTGATAACTACA‑TCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTTCCTCcaca                                                                                                                                 <  1:407453/238‑1 (MQ=255)                                                                                                  cAATTTCTATTTCTAAAATCATTGGCGGTTGCGGTCCTACTGCACAGTAAGTCTCTGACAGAAATCGGCTAACA‑CCGGTTTAATTGCCCGACGCCCTCT‑GGATTTTCCGAGGGCGTA‑TTTGTTT‑CTATGT‑C‑CT‑TCTTGC‑CCCC‑GCCCGTAAAAATA‑TTTTCACTTTAAATTCAATTTGATAACTACA‑TCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTTCCTCcaca                                                                                                                                 <  2:104351/238‑1 (MQ=255)                                                                                                      ttctatttctaAAATCATTGGCGGTTGCGGTCCTACTGCACAGTAAGTCTCTGACAGAAATCGGCTAACA‑CCGGTTTAATTGCCCGACGCCCTCT‑GGATTTTCCGAGGGCGTA‑TTTGTTT‑CTATGT‑C‑CT‑TCTTGC‑CCCC‑GCCCGTAAAAATA‑TTTTCACTTTAAATTCAATTTGATAACTACA‑TCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTTCCTCCACATTACa                                                                                                                            <  1:398125/239‑1 (MQ=255)                                                                                                       tctatttctaAAATCATTGGCGGTTGCGGTCCTACTGCACAGTAAGTCTCTGACAGAAATCGGCTAACA‑CCGGTTTAATTGCCCGACGCCCTCT‑GGATTTTCCGAGGGCGTA‑TTTGTTT‑CTATGT‑C‑CT‑TCTTGC‑CCCC‑GCCCGTAAAAATA‑TTTTCACTTTAAATTCAATTTGATAACTACA‑TCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTTCCTCCACATTACAt                                                                                                                           <  1:282991/239‑1 (MQ=255)                                                                                                       tctatttctaAAATCATTGGCGGTTGCGGTCCTACTGCACAGTAAGTCTCTGACAGAAATCGGCTAACA‑CCGGTTTAATTGCCCGACGCCCTCT‑GGATTTTCCGAGGGCGTA‑TTTGTTT‑CTATGT‑C‑CT‑TCTTGC‑CCCC‑GCCCGTAAAAATA‑TTTTCACTTTAAATTCAATTTGATAACTACA‑TCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTTCCTCCACATTACAt                                                                                                                           <  1:923116/239‑1 (MQ=255)                                                                                                       tctatttctaAAATCATTGGCGGTTGCGGTCCTACTGCACAGTAAGTCTCTGACAGAAATCGGCTAACA‑CCGGTTTAATTGCCCGACGCCCTCT‑GGATTTTCCGAGGGCGTA‑TTTGTTT‑CTATGT‑C‑CT‑TCTTGC‑CCCC‑GCCCGTAAAAATA‑TTTTCACTTTAAATTCAATTTGATAACTACA‑TCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTTCCTCCACATTACAt                                                                                                                           <  1:1168895/239‑1 (MQ=255)                                                                                                        ctatttctaAAATCATTGGCGGTTGCGGTCCTACTGCACAGTAAGTCTCTGACAGAAATCGGCTAACA‑CCGGTTTAATTGCCCGACGCCCTCT‑GGATTTTCCGAGGGCGTA‑TTTGTTT‑CTATGT‑C‑CT‑TCTTGC‑CCCC‑GCCCGTAAAAATA‑TTTTCACTTTAAATTCAATTTGATAACTACA‑TCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTTCCTCCACATTACATa                                                                                                                          <  1:704276/239‑1 (MQ=255)                                                                                                        ctacttctaAAATCATTGGCGGTGTCGGTCCTACTGCACAGTAAGTCTCTGACAGAAATCGGCTAACA‑ACGGTTTAATTGCCCGACGCCCTCT‑GGAGTTTCCGAGGGCGTA‑TTTGTTT‑CTATGT‑C‑CT‑TCTTGC‑CCCC‑GCCCGTAAAAATA‑TTTTCTCTTTAAATTCAATTTGATAACTACA‑TCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTTCCTCCACATTACATa                                                                                                                          <  2:858327/239‑1 (MQ=255)                                                                                                         tatttctaAAATCATTGGCGGTTGCGGTCCTACTGCACAGTAAGTCTCTGACAGAAATCGGCTAACA‑CCGGTTTAATTGCCCGACGCCCTCT‑GGATTTTCCGAGGGCGTA‑TTTGTTT‑CTATGT‑C‑CT‑TCTTGC‑CCCC‑GCCCGTAAAAATA‑TTTTCACTTTAAATTCAATTTGATAACTACA‑TCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTTCCTCCACATTACATaa                                                                                                                         <  1:1103166/239‑1 (MQ=255)                                                                                                               taAAATCATTGGCGGTTGCGGTCCTACTGCACAGTAAGTCTCTGACAGAAATCGGCTAACA‑CCGGTTTAATTGCCCGACGCCCTCT‑GGATTTTCCGAGGGCGTA‑TTTGTTT‑CTATGT‑C‑CT‑TCTTGC‑CCCC‑GCCCGTAAAAATA‑TTTTCACTTTAAATTCAATTTGATAACTACA‑TCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTTCCTCCACATTACATAAcatcat                                                                                                                   <  2:139137/239‑1 (MQ=255)                                                                                                                aaaaTCATTGGCGGTTGCGGTCCTACTGCACAGTAAGTCTCTGACAGAAATCGGCTAACA‑CCGGTTTAATTGCCCGACGCCCTCT‑GGATTTTCCGAGGGCGTA‑TTTGTTT‑CTATGT‑C‑CT‑TCTTGC‑CCCC‑GCCCGTAAAAATA‑TTTTCACTTTAAATTCAATTTGATAACTACA‑TCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTTCCTCCACATTACATAACATCata                                                                                                                  <  1:428754/239‑1 (MQ=255)                                                                                                                  aagcATTGGCGGTTGCGGTGCTACTGCACAGTTAGTCTCTGACAGAAATCGGCTAACA‑CCGGTTTAATTGCCCGACGCCCTCT‑GGATTTTCCGATGGCGTA‑TTTGTTT‑CTATGT‑C‑CT‑TCTTGC‑CCCC‑TCCCGTAAAAATA‑TTTTCACTTTAAATTCAATTTGATAACTACA‑TCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTTCCTCCACATTACATAACATCATAtg                                                                                                                <  2:1081316/236‑1 (MQ=255)                                                                                                                  aaTCATTGGCGGTTGCGGTCCTACTGCACAGTAAGTCTCTGACAGAAATCGGCTAACA‑CCGGTTTAATTGCCCGACGCCCTCT‑GGATTTTCCGAGGGCGTA‑TTTGTTT‑CTATGT‑C‑CT‑TCTTGC‑CCCC‑GCCCGTAAAAATA‑TTTTCACTTTAAATTCAATTTGATAACTACA‑TCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTTCCTCCACATTACATAACATCATAtg                                                                                                                <  2:460045/239‑1 (MQ=255)                                                                                                                  aaTCATTGGCGGTTGCGGTCCTACTGCACAGTAAGTCTCTGACAGAAATCGGCTAACA‑CCGGTTTAATTGCCCGACGCCCTCT‑GGATTTTCCGAGGGCGTA‑TTTGTTT‑CTATGT‑C‑CT‑TCTTGC‑CCCC‑GCCCGTAAAAATA‑TTTTCACTTTAAATTCAATTTGATAACTACA‑TCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTTCCTCCACATTACATAACATCATAtg                                                                                                                <  1:270167/239‑1 (MQ=255)                                                                                                                  aaTCATTGGCGGTTGCGGTCCTACTGCACAGTAAGTCTCTGACAGAAATCGGCTAACA‑CCGGTTTAATTGCCCGACGCCCTCT‑GGATTTTCCGAGGGCGTA‑TTTGTTT‑CTATGT‑C‑CT‑TCTTGC‑CCCC‑GCCCGTAAAAATA‑TTTTCACTTTAAATTCAATTTGATAACTACA‑TCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTTCCTCCACATTACATAACATCATAtg                                                                                                                <  1:206723/239‑1 (MQ=255)                                                                                                                  aaTCATTGGCGGTTGCGGTCCTACTGCACAGTAAGTCTCTGACAGAAATCGGCTAACA‑CCGGTTTAATTGCCCGACGCCCTCT‑GGATTTTCCGAGGGCGTA‑TTTGTTT‑CTATGT‑C‑CT‑TCTTGC‑CCCC‑GCCCGTAAAAATA‑TTTTCACTTTAAATTCAATTTGATAACTACA‑TCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTTCCTCCACATTACATAACATCATAtg                                                                                                                <  1:314089/239‑1 (MQ=255)                                                                                                                           cGGTTCCGGTCCTACTGCACAGTAAGTCTCTGACAGAAATCGGCTAACT‑CCGGTTTAATTGCCCGACGCCCTCT‑GGATTTTCCGAGGGCGTA‑TTTGTTT‑CTATGT‑C‑CT‑TCTTGC‑CCCC‑GCCCGTAAAAATA‑TTTTCACTTTAAATTCAATTTGATAACTACA‑TCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTTCCTCCACATTACATAACATCATATGTTGTtata                                                                                                        <  2:1057355/238‑1 (MQ=255)                                                                                                                                gccgTCCTACTGCACAGTAAGTCTCTTACAGAAATCGGCTAACA‑CCGGTTTAATTGCCCGACGCCCTCT‑GGATTTTCCGAGGGCGTA‑TTTGTTT‑CTATGT‑C‑CT‑TCTTGC‑CCCC‑GCCCGTAAAAATA‑TTTTCACTTTAAATTCAATTTGATAACTACA‑TCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTTCCTCCACATTACATAACATCATATGTTGTTATATtcatc                                                                                                  <  2:430131/236‑1 (MQ=255)                                                                                                                                      cTACTGCACAGTAAGTCTCTGACAGAAATCGGCTAACA‑CCTGTTTAATTGCCCGACGCCCTCT‑GGATTTTCCGAGGGCGTA‑TTTGTTT‑CTATGT‑C‑CT‑TCTTGC‑CCCC‑GCCCGTAAAAATA‑TTTTCACTTTAAATTCAATTTGATAACTACA‑TCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTTCCTCCACATTACATAACATCATATGTTGTTATATTCATCATGCAt                                                                                            <  2:512750/239‑1 (MQ=255)                                                                                                                                                  aaGTCTCTGACAGAAATCGGCTAACA‑CCGGTTTAATTGCCCGACGCCCTCT‑GGATTTTCCGAGGGCGTA‑TTTGTTT‑CTATGT‑C‑CT‑TCTTGC‑CCCC‑GCCCGTAAAAATA‑TTTTCACTTTAAATTCAATTTGATAACTACA‑TCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTTCCTCCACATTACATAACATCATATGTTGTTATATTCATCATGCATTGTCATGTTa                                                                                  <  1:60123/237‑1 (MQ=255)                                                                                                                                                   aGTCTCTGACAGAAATCGGCTAACA‑CCGGTTTAATTGCCCGACGCCCTCT‑GGATTTTCCGAGGGCGTA‑TTTGTTT‑CTATGT‑C‑CT‑TCTTGC‑CCCC‑GCCCGTAAAAATA‑TTTTCACTTTAAATTCAATTTGATAACTACA‑TCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTTCCTCCACATTACATAACATCATATGTTGTTATATTCATCATGCATTGTCATGTTACCt                                                                               >  1:841787/1‑239 (MQ=255)                                                                                                                                                    gTCTCTGACAGAAATCGGCTAACA‑CCGGTTTAATTGCCCGACGCCCTCT‑GGATTTTCCGAGGGCGTA‑TTTGTTT‑CTATGT‑C‑CT‑TCTTGC‑CCCC‑GCCCGTAAAAATA‑TTTTCACTTTAAATTCAATTTGATAACTACA‑TCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTTCCTCCACATTACATAACATCATATGTTGTTATATTCATCATGCATTGTCATGTTACCtt                                                                              >  1:796578/1‑239 (MQ=255)                                                                                                                                                      ctctGACAGAAATCGGCTAACA‑CCGGTTTAATTGCCCGACGCCCTCT‑GGATTTTCCGAGGGCGTA‑TTTGTTT‑CTATGT‑C‑CT‑TCTTGC‑CCCC‑GCCCGTAAAAATA‑TTTTCACTTTAAATTCAATTTGATAACTACA‑TCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTTCCTCCACATTACATAACATCATATGTTGTTATATTCATCATGCATTGTCATGTTACCtttt                                                                            >  1:543001/1‑239 (MQ=255)                                                                                                                                                           aCAGAAATCGGCTAACA‑CCGGTTTAATTGCCCGACGCCCTCT‑GGATTTTCCGAGGGCGTA‑TTTGTTT‑CTATGT‑C‑CT‑TCTTGC‑CCCC‑GCCCGTAAAAATA‑TTTTCACTTTAAATTCAATTTGATAACTACA‑TCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTTCCTCCACATTACATAACATCATATGTTGTTATATTCATCATGCATTGTCATGTTACCTTTTAAATg                                                                       >  1:935959/1‑239 (MQ=255)                                                                                                                                                                   cGGCTAACA‑CCTGTTTAATTGCCCGTCGCCCTCT‑GGATTTTCCGAGGGCGTA‑TTTGTTT‑CTATGT‑C‑CT‑TCTTGC‑CCCC‑GCCCGTAAAAATA‑TTTTCACTTTAAATTCAATTTGATAACTACA‑TCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTTCCTCCACATTACATAACATCATATGTTGTTATATTCATCATGCATTGTCATGTTACCTTTTAAATGACTGCaaa                                                               <  2:1037896/239‑1 (MQ=255)                                                                                                                                                                     gCTAACA‑CCGGTTTAATTGCCCGACGCCCTCT‑GGATTTTCCGAGGGCGTA‑TTTGTTT‑CTATGT‑C‑CT‑TCTTGC‑CCCC‑GCCCGTAAAAATA‑TTTTCACTTTAAATTCAATTTGATAACTACA‑TCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTTCCTCCACATTACATAACATCATATGTTGTTATATTCATCATGCATTGTCATGTTACCTTTTAAATGACTGCAAAc                                                              >  2:502688/1‑238 (MQ=255)                                                                                                                                                                      cTAACT‑CCGGTTTAATTGCCCGACGCCCTCT‑GGATTTTCCGAGGGCGTA‑TTTGTTT‑CTATGT‑C‑CT‑TCTTGC‑CCCC‑GCCCGTAAAAATA‑TTTTCACTTTAAATTCAATTTGATAACTACA‑TCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTTCCTCCACATTACATAACATCATATGTTGTTATATTCATCATGCATTGTCATGTTACCTTTTAAATGACTGCAAAct                                                             <  2:1102114/238‑1 (MQ=255)                                                                                                                                                                      cTAACA‑CCGGTTTAATTGCCCGACGCCCTCT‑GGATTTTCCGAGGGCGTA‑TTTGTTT‑CTATGT‑C‑CT‑TCTTGC‑CCCC‑GCCCGTAAAAATA‑TTTTCACTTTAAATTCAATTTGATAACTACA‑TCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTTCCTCCACATTACATAACATCACATGTTGTTATATTCATCATGCATTGTCATGTTACCTTTTAAATGACTGCAAAct                                                             <  1:346388/238‑1 (MQ=255)                                                                                                                                                                        aaCA‑CCGGTTTAATTGCCCGACGCCCTCT‑GGATTTTCCGAGGTCGTA‑TTTGTTT‑CTATGT‑C‑CT‑TCTTGC‑CCCC‑GCCCGTAAAAATA‑TTTTCACTTTAAATTCAATTTGATAACTACA‑TCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTTCCTCCACATTACATAACATCATATGTTGTTATATTCATCATGCATTGTCATGTTACCTTTTAAATGACTGCAAACtctc                                                          <  2:518172/239‑1 (MQ=255)                                                                                                                                                                          ca‑cCGGTTTAATTGCCCGACGCCCTCT‑GGATTTTCCGAGGGCGTA‑TTTGTTT‑CTATGT‑C‑CT‑TCTTGC‑CCCC‑GCCCGTAAAAATA‑TTTTCACTTTAAATTCAATTTGATAACTACA‑TCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTTCCTCCACATTACATAACATCATATGTTGTTATATTCATCATGCATTGTCATGTTACCTTTTAAATGACTGCAAACTCTccc                                                        <  1:502688/239‑1 (MQ=255)                                                                                                                                                                          ca‑cCGGTTTAATTGCCCGACGCCCTCT‑GGATTTTCCGAGGGCGTA‑TTTGTTT‑CTATGT‑C‑CT‑TCTTGC‑CCCC‑GCCCGTAAAAATA‑TTTTCACTTTAAATTCAATTTGATAACTACA‑TCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTTCCTCCACATTACATAACATCATATGTTGTTATATTCATCATGCATTGTCATGTTACCTTTTAAATGACTGCAAACTCTccc                                                        <  2:935959/239‑1 (MQ=255)                                                                                                                                                                              ctgTTTAATTGCCCGACGACCTCT‑GGATTTTACGAGGGCGTA‑TGTGTTT‑CTATTT‑A‑CT‑TCTTGC‑CCAC‑GCCCGTAAAAATA‑TTTTCACTTTAAATTCAATTTGATAACTACA‑TCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTTCCTCCACATTACATAACATCATATGTTGTTATATTCATCATGCATTGTCATGTTACCTTTTAAATGACTGCAAACTCTCCCCTa                                                     <  2:561552/237‑1 (MQ=255)                                                                                                                                                                                gTTTAATTGCCCGACGCCCTCT‑GGATTTTCCGAGGGCGTA‑TTTGTTT‑CTATGT‑C‑CT‑TCTTGC‑CCCC‑GCCCGTAAAAATA‑TTTTCACTTTAAATTCAATTTGATAACTACA‑TCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTTCCTCCACATTACATAACATCATATGTTGTTATATTCATCATGCATTGTCATGTTACCTTTTAAATGACTGCAAACTCTCCCCTACa                                                   >  2:432987/1‑239 (MQ=255)                                                                                                                                                                                     aTTGCCCGACGCCCTCT‑GGATTTTCCGAGGTCGTA‑TTTGTTT‑CTATTT‑C‑CT‑TCTTGC‑CCCC‑GCCCGTAAAAATA‑TTTTCACTTTAAATTCAATTTGATAACTACA‑TCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTTCCTCCACATTACATAACATCATATGTTGTTATATTCATCATGCATTGTCATGTTACCTTTTAAATGACTGCAAACTCTCCCCTACAACGTa                                              <  1:546850/239‑1 (MQ=255)                                                                                                                                                                                     aTTGCCCGACGCCCTCT‑GGATTTTCCGAGGGCGTA‑TTTGTTT‑CTATGT‑C‑CT‑TCTTGC‑CCCC‑GCCCGTAAAAATA‑TTTTCACTTTAAATTCAATTTGATAACTACA‑TCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTTCCTCCACATTACATAACATCATATGTTGTTATATTCATCATGCATTGTCATGTTACCTTTTAAATGACTGCAAACTCTCCCCTACAACGTa                                              >  1:141026/1‑239 (MQ=255)                                                                                                                                                                                         cccGACGCCCTCT‑GGATTTTCCGAGGGCGTA‑TTTGTTT‑CTATGT‑C‑CT‑TCTTGC‑CCCC‑GCCCGTAAAAATA‑TTTTCACTTTAAATTCAATTTGATAACTACA‑TCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTTCCTCCACATTACATAACATCATATGTTGTTATATTCATCATGCATTGTCATGTTACCTTTTAAATGACTGCAAACTCTCCCCTACAACGTATTgg                                          >  1:847273/1‑239 (MQ=255)                                                                                                                                                                                                         aTTTTCCGAGGGCGTA‑TTTGTTT‑CTATGT‑C‑CT‑TCTTGC‑CCCC‑GCCCGTAAAAATA‑TTTTCACTTTAAATTCAATTTGATAACTACA‑TCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTTCCTCCACATTACATAACATCATATGTTGTTATATTCATCATGCATTGTCATGTTACCTTTTAAATGACTGCAAACTCTCCCCTACAACGTATTGGACAAGAAAAAGGTa                            >  2:67701/1‑238 (MQ=255)                                                                                                                                                                                                            ttCCGAGGGCGTA‑TTTGTTT‑CTATGT‑C‑CT‑TCTTGC‑CCCC‑GCCCGTAAAAATA‑TTTTCACTTTAAATTCAATTTGATAACTACA‑TCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTTCCTCCACATTACATAACATCATATGTTGTTATATTCATCATGCATTGTCATGTTACCTTTTAAATGACTGCAAACTCTCCCCTACAACGTATTGGACAAGAAAAAGGTATCGc                        <  1:461796/239‑1 (MQ=255)                                                                                                                                                                                                             tCCGAGGGCGTA‑TTTGTTT‑CTATGT‑C‑CT‑TCTTGC‑CCCC‑GCCCGTAAAAATA‑TTTTCACTTTAAATTCAATTTGATACCTACA‑TCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTTCCTCCACATTACATAACATCATATGTTGTtata                                                                                                        <  2:538948/158‑1 (MQ=255)                                                                                                                                                                                                             tCCGAGGGCGTA‑TTTGTTT‑CTATGT‑C‑CT‑TCTTGC‑CCCC‑GCCCGTAAAAATA‑TTTTCACTTTAAATTCAATTTGATAACTACA‑TCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTTCCTCCACATTACATAACATCATATGTTGTtata                                                                                                        >  1:538948/1‑158 (MQ=255)                                                                                                                                                                                                                    gCGTA‑TTTGTTT‑CTATGT‑C‑CT‑TCTTGC‑CCCC‑GCCCGTAAAAATA‑TTTTCACTTTAAATTCAATTTGATAACTACA‑TCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTTCCTCCACATTACATAACATCATATGTTGTTATATTCATCATGCATTGTCATGTTACCTTTTAAATGACTGCAAACTCTCCCCTACAACGTATTGGACAAGAAAAAGGTATCGCTATggg                  <  2:543001/237‑1 (MQ=255)                                                                                                                                                                                                                           ttGTTT‑CTATGT‑C‑CT‑TCTTGC‑CCCC‑GCCCGTAAAAATA‑TTTTCACTTTAAATTCAATTTGATAACTACA‑TCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTTCCTCCACATTACATAACATCATATGTTGTTATATTCATCATGCATTGTCATGTTACCTTTTAAATGACTGCAAACTCTCCCCTACAACGTATTGGACAAGAAAAAGGTATCGCTATGGGAAGCCAgg          <  1:676735/239‑1 (MQ=255)                                                                                                                                                                                                                               tt‑CTATGT‑C‑CT‑TCTTGC‑CCCC‑GCCCGTAAAAATA‑TTTTCACTTTAAATTCAATTTGATAACTACA‑TCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTTCCTCCACATTACATAACATCATATGTTGTTATATTCATCATGCATTGTCATGTTACCTTTTAAATGACTGCAAACTCTCCCCTACAACGTATTGGACAAGAAAAAGGTATCGCTATGGGAAGCCAGGAAc       >  1:1153450/1‑238 (MQ=255)                                                                                                                                                                                                                                t‑CTATGT‑C‑CT‑TCTTGC‑CCCC‑GCCCGTAAAAATA‑TTTTCACTCTAAATTCAATTAGATAACTACA‑TCCATTAGCAACAGCGCGATCCTGTGCGAAATTTTCTGCTCTTCCTCCACATTACATAACATCATATGTTGTTATATTCATCATGCTTTGTCATGTTACCTTTTAAATGACTGCAAACTCTCCACTCCAAAGTATTGACCAAGAAAAAGTTATAGCTATGGGATGCCAGGTACt      >  2:1095406/1‑238 (MQ=255)                                                                                                                                                                                                                                     tGT‑C‑CT‑TCTTGC‑CCCC‑GCCCGTAAAAATA‑TTTTCACTTTATATTCAATTTGATAACTACA‑TACATTAGCCACAACGCGATCCTTTGCGAAATTTTGTGATCTTCCTCCACATTACATAACATCATATGTTGTTATATTCATCATGCATTGTCATGATACCTTTTAAATGACTGCAAACTCTCCCCTACAACGTATTGGACAAGAAAAAGCTATCGCTATGGGAAGCCAGGACCTCCaa  >  2:178940/1‑238 (MQ=255)                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                    GCACTGGTAGGTGCAGTACTGGCTACGTTAACTGCATGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTGCGGTCCTACTGCACAGTAAGTCTCTGACAGAAATCGGCTAACA‑CCGGTTTAATTGCCCGACGCCCTCT‑GGATTTTCCGAGGGCGTA‑TTTGTTT‑CTATGT‑C‑CT‑TCTTGC‑CCCC‑GCCCGTAAAAATA‑TTTTCACTTTAAATTCAATTTGATAACTACA‑TCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTTCCTCCACATTACATAACATCATATGTTGTTATATTCATCATGCATTGTCATGTTACCTTTTAAATGACTGCAAACTCTCCCCTACAACGTATTGGACAAGAAAAAGGTATCGCTATGGGAAGCCAGGAACTCCAA  >  NC_000913/3499469‑3499930

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑