Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	509,371	A→C	16.7%	V670G (GTG→GGG)	copA ←	copper transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	509,371	0	A	C	16.7%	180.5 / 3.7	77	V670G (GTG→GGG)	copA	copper transporter
Reads supporting (aligned to +/- strand):  ref base A (31/29);  new base C (6/9);  total (37/40)
Fisher's exact test for biased strand distribution p-value = 5.65e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGCGGTTTCAATGGCAACATCACTGCCGCCACCCATCGCAATGCCGACATCCGCCTGAGCCAGCGCTGGCGCGTCGTTAATGCCGTCGCCCACCATTGCCACCTGACGTCCTTCACTTTGCAGATGTTTGATCGCTTCGGCTTTACCGTCCGGCAGCACCCCGGCGATCACCTCATCAATCCCTGCTTCTTTGGC‑GATCGCATTGGCGGTGGTTGGGTTATCCCCGGTCAACATCACCAGACGATATCCCGCTTTATGC‑AGGCGTTGCA‑GCGCCGCCACGCTATCACTACGCAACGGATCGCGTACTGCCAGCAGGGCTACCGCTTTCCCGTCAACCGCCAGCAGCACAGGCGT‑TGCCCCTTGCGATGCCTGAGCAGTAATCTCCGCTTCGATAGCTTTGGTACCAACCTGTTGCTCATTTAACAGCGCCTGATTGCCCAGCAATAACGCATGACCTTCAGCTTCACCGCT  >  NC_000913/509136‑509606                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                               cgcgGTTTCAATGGCAACATCACTGCCGCCACCCATCGCAATGCCGACATCCGCCTGAGCCAGCGCTGGCGCGTCGTTAATGCCGTCGCCCACCATTGCCACCTGACGTCCTTCACTTTGCAGATGTTTGATCGCTTCGGCTTTACCGTCCGGCAGCACCCCGGCGATCACCTCATCAATCCCTGCTTCTTTGGC‑GATCGCATTGGCGGTGGTTGGGTTATCCCCGGTCAACATCAcc                                                                                                                                                                                                                                              <  1:238082/238‑1 (MQ=255)   cgGTTTCAATGGCAAACTCACTGCCGCCACCCATCGCAATGCCCACATCCGCCTGAGCCAGCGCTGGCGCGTCGTTAATGCCGTCGCCCACCATTGCCACCTGACGTCCTTCACTTTGCAGATGTTTGATCGCTTCGGCTTTACCGTCCGGCAGCACCCCGGCGATCACCTCATCAATCCCTGCTTCTTTGGC‑GATCGCATTGGCGGTGGTTGGGTTATCCCCGGTCAACATCACCAGa                                                                                                                                                                                                                                           <  1:625333/239‑1 (MQ=255)      tttCAATGGCAACACCACTGCCGCCCCCCATCGCAATGCCGCCATCCGCCTGAGCCAGCGCTGGCGCGTCGTTAATGCCGTCGCCCACCATTGCCACCTGACGTCCTTCACTTTGCAGATGTTTGATCGCTTCGGCTTTACCGTCCGGCAGCACCCCGGCGATCACCTCATCAATCCCTGCTTCTTTGGC‑GATCGCATTGGCGGTGGTTGGGTTATCCCCGGTCAACATCACCAGACg                                                                                                                                                                                                                                         <  2:1188289/238‑1 (MQ=255)          aaTGGCAACATCACTGCCGCCACCCATCGCAATGCCGACATCCGCCTGAGCCAGCGCTGGCGCGTCGTTAATGCCGTCGCCCACCATTGCCACCTGACGTCCTTCACTTTGCAGATGTTTGATCGCTTCGGCTTTACCGTCCGGCAGCACCCCGGCGATCACCTCATCAATCCCTGCTTCTTTGGC‑GATCGCATTGGCGGTGGTTGGGTTATCCCCGGTCAACATCACCAGACGATATc                                                                                                                                                                                                                                    <  1:572031/239‑1 (MQ=255)          aaTGGCAACATCACTGCCGCCACCCATCGCAATGCCGACATCCGCCTGAGCCAGCGCTGGCGCGTCGTTAATGCCGTCGCCCACCATTGCCACCTGACGTCCTTCACTTTGCAGATGTTTGATCGCTTCGGCTTTACCGTCCGGCAGCACCCCGGCGATCACCTCATCAATCCCTGCTTCTTTGGC‑GATCGCATTGGCGGTGGTTGGGTTATCCCCGGTCAACATCACCAGACGATATc                                                                                                                                                                                                                                    <  1:530675/239‑1 (MQ=255)          aaTGGCAACATCACTGCCGCCACCCATCGCAATGCCGACATCCGCCTGAGCCAGCGCTGGCGCGTCGTTAATGCCGTCGCCCACCATTGCCACCTGACGTCCTTCACTTTGCAGATGTTTGATCGCTTCGGCTTTACCGTCCGGCAGCACCCCGGCGATCACCTCATCAATCCCTGCTTCTTTGGC‑GATCGCATTGGCGGTGGTTGGGTTATCCCCGGTCAACATCACCAGACGATATc                                                                                                                                                                                                                                    <  1:363860/239‑1 (MQ=255)              gCAACATCACTGCCGCCACCCATCGCAATGCCGACATCCGCCTGAGCCAGCGCTGGCGCGTCGTTAATGCCGTCGCCCACCATTGCCACCTGACGTCCTTCACTTTGCAGATGTTTGATCGCTTCGGCTTTACCGTCCGGCAGCACCCCGGCGATCACCTCATCAATCCCTGCTTCTTTGGC‑GATCGCATTGGCGGTGGTTGGGTTATCCCCCGCCAACATCCCCAGACCCTATcccc                                                                                                                                                                                                                                 >  2:329083/1‑237 (MQ=255)                        tGCCGCCACCCATCGCAATGCCGACATCCGCCTGAGCCAGCGCTGGCGCGTCGTTAATGCCGTCGCCCACCATTGCCACCTGACGTCCTTCACTTTGCAGATGTTTGATCGCTTCGGCTTTACCGTCCGGCAGCACCCCGGCGATCACCTCATCAATCCCTGCTTCTTTGGC‑GATCGCCTTGGCGGTGGTTGGGTTATCCCCGGTCCACACTACCCGACGATTTCCCGATTTATGC‑Ag                                                                                                                                                                                                                      >  2:1193631/1‑238 (MQ=255)                                  cATCGCAATGCCGACATCCGCCTGAGCCAGCGCTGGCGCGTCGTTAATGCCGTCGCCCACCATTGCCACCTGACGTCCTTCACTTTGCAGATGTTTGATCGCTTCGGCTTTACCGTCCGGCAGCACCCCGGCGATCACCTCATCAATCCCTGCTTCTTTGGC‑GATCGCATTGGCGGTGGTTGGGTTATCCCCGGGCAACATTCCCAGACGATCTCCCGCTTTTTGC‑CCGCGTTggggc                                                                                                                                                                                                            >  2:324303/1‑234 (MQ=255)                                      gCAATGCCGCCATCCGCCGGCGCCAGCGCTGGCGCGTCGTTAATGCCGTCGCCCACCATTGCCACCTGACGTCCTTCACTTTGCAGATGTTTGATCGCTTCGGCTTTACCGTCCGGCAGCACCCCGGCGATCCCCTCATCAATCCCTGCTTCTTTGGC‑GATCGCATTGGCGGTGGTTGGGTTATCCCCGGTCAACATCACCAGACGATATCCCGCTTTATGC‑AGGCGTTGCA‑Gcgccgc                                                                                                                                                                                                      <  2:535352/239‑1 (MQ=255)                                      gCAATGCCGACATCCGCCTGAGCCAGCGCTGGCGCGTCGTTAATGCCGTCGCCCACCATTGCCACCTGACGTCCTTCACTTTGCAGATGTTTGATCGCTTCGGCTTTACCGTCCGGCAGCACCCCGGCGATCACCTCATCAATCCCTGCTTCTTTGGC‑GATCGCATTGGCGGTGGTTGGGTTATCCCCGGTCAACATCACCAGACGATATCCCGCTTTATGC‑AGGCGTTGCA‑Gcgccgc                                                                                                                                                                                                      <  2:1022808/239‑1 (MQ=255)                                      gCAATGCCGACATCCGCCTGAGCCAGCGCTGGCGCGTCGTTAATGCCGTCGCCCACCATTGCCACCTGACGTCCTTCACTTTGCAGATGTTTGATCGCTTCGGCTTTACCGTCCGGCAGCACCCCGGCGATCACCTCATCAATCCCTGCTTCTTTGGC‑GATCGCATTGGCGGTGGTTGGGTTATCCCCGGTCAACATCACCAGACGATATCCCGCTTTATGC‑AGGCGTTGCA‑Gcgccgc                                                                                                                                                                                                      <  2:706737/239‑1 (MQ=255)                                      gCAATGCCCCCCTCCGCCTGAGCCAGCGCTGGCGCGTCGTTAATGCCGTCGCCCACCATTGCCACCTGACGTCCTTCACTTTGCAGATGTTTGATCGCTTCGGCTTTACCGTCCGGCAGCACCCCGGCGATCACCTCATCAATCCCTGCTTCTTTGGC‑GATCGCATTGGCGGTGGTTGGGTTATCCCCGGTCAACATCACCAGACGATATCCCGCTTTATGC‑AGGCGTTGCA‑Gcgccgc                                                                                                                                                                                                      <  2:1019360/239‑1 (MQ=255)                                        aaTGCCGCCACCCGCCTGAGCCAGCGCTGGCGCGTCGTTAAGGCCTTCGCCCACCATTGCCCCCTGACGTCCTGCACTGTGCAGATGTTTGATCGCTTCGGCTTTACCGTCCGGCAGCACCCCGGCGATCACCTCATCAATCCCTGCTTCTTTGGC‑GATCGCATTGGCGGTGGTTGGGTTATCCCCGGTCAACATCACCAGACGATATCCCGCTTTACGC‑AGGCGTTGCA‑GCGCCGCCa                                                                                                                                                                                                    <  2:900425/239‑1 (MQ=255)                                        aaTGCCGACATCCGCCTGAGCCAGCGCTGGCGCGTCGTTAATGCCGTCGCCCACCATTGCCACCTGACGTCCTTCACTTTGCAGATGTTTGATCGCTTCGGCTTTACCGTCCGGCAGCACCCCGGCGATCACCTCATCAATCCCTGCTTCTTTGGC‑GATCGCATTGGCGGTGGTTGGGTTATCCCCGGTCAACATCACCAGACGATATCCCGCTTTATGC‑AGGCGTTGCA‑GCGCCGCCa                                                                                                                                                                                                    <  1:1010375/239‑1 (MQ=255)                                           gCCGACATCCGCCTGAGCCAGCGCTGGCGCGTCGTTAATGCCGTCGCCCACCATTGCCACCTGACGTCCTTCACTTTGCAGATGTTTGATCGCTTCGGCTTTACCGTCCGGCAGCACCCCGGCGATCACCTCATCAATCCCTGCTTCTTTGGC‑GATCGCATTGGCGGTGGTTGGGTTATCCCCCGTCAACATCACCAGACGATATCCCGCTTTATGC‑AGGCGGTGCA‑GCGCCGCCACg                                                                                                                                                                                                  >  1:420089/1‑238 (MQ=255)                                            ccGCCATCCGCCTGAGCCAGCGCTGGCGCGTCGTGAATGCCGTCGCCCGCCATTGCCACCTGACGTCCTTCACTTTGCAGATTTTTGATCGCTTCGGCTTTCCCGTCCGGCAGCACCCCGGCGATCACCTCATCAATCCCTGCTTCTTGGGC‑GATCGCATTGGCGGTGGTTGGGTTATACCCGGTCAACATCACCAGACGATATCCCGCTTTATGC‑AGGCGTTGCA‑GCGCCGCCACGc                                                                                                                                                                                                 <  2:884586/238‑1 (MQ=255)                                            ccGACATCCGCCTGAGCCAGCGCTGGCGCGTCGTTAATGCCGTCGCCCACCATTGCCACCTGACGTCCTTCACTTTGCAGATGTTTGATCGCTTCGGCTTTACCGTCCGGCAGCGCCCCGGCGATCACCTCATCAATCCCTGCTTCTTTGGC‑GATCGCATTGGCGGTGGTTGGGTTATCCCCGGGCAACATCACCAGACGATATCCCGCTTTATGC‑AGGCGTTGCA‑GCGCCGCCACGc                                                                                                                                                                                                 <  2:70036/238‑1 (MQ=255)                                              gACATCCGCCTGAGCCAGCGCTGGCGCGTCGTTAATGCCGTCGCCCACCATTGCCACCTGACGTCCTTCACTTTGCAGATGTTTGATCGCTTCGGCTTTACCGTCCGGCAGCACCCCGGCGATCACCTCATCAATCCCTGCTTCTTTGGC‑GATCGCATTGGCGGTGGTTGGGTTATCCCCGGTCAACATCACCAGACGATATCCCGCTTTATGC‑AGGCGTTGCA‑GCGCCGCCACGCTAt                                                                                                                                                                                              <  2:21688/239‑1 (MQ=255)                                               aCATCCGCCTGAGCCAGCGCTGGCGCGTCGTTAATGCCGTCGCCCACCATTGCCACCTGACGTCCTTCACTTTGCAGATGTTTGATCGCTTCGGCTTTACCGTCCGGCAGCACCCCGGCGATCACCTCATCAATCCCTGCTTCTTTGGC‑GATCGCATTGGCGGTGGTTGGGTTATCCCCGGGCCACATCACCCAACGATATCCCGCTTTATCC‑AGGCCTTGCC‑GCgccccccccccct                                                                                                                                                                                              >  2:874002/1‑231 (MQ=255)                                                 gtctcccTGCGCCAGCCCTGGCGCGTCGTTACTGCCGTGGCCCACCATTGCCACCGGCCGTCCTTCACTTTGCAGATGTTTGCTCGCTACGGCTTTGCCGTCCGGCAGCACCCCGGGGATCACCTCATCAATCCCTGCTTCTTTGGC‑GAGCGCATTTGCGGTGGTTGGGTTATCCCCGGTCAACATCACCAGAGGATAGCCCGCTTTATGC‑AGGCGTTGCA‑GCGCCGCCACGCTATCa                                                                                                                                                                                            <  2:99491/233‑1 (MQ=255)                                                  tCCGCCTGAGCCAGCGCTGGCGCGTCGTTAATGCCGTCGCCCGCCATTGCCCCCTGACGCCCTTCCCTTTGCAGATGTTTGATCGCTTCGGCTTTACCGTCCGGCAGCACCCCGGCGATCACCTCATCAATCCCTGCTTCTTTGGC‑GATCGCATTGGCGGTGGTTGGTTTATCCCCGGTCAACATCACCAGACGATATCCCGCTTTATGC‑AGGCGTTGCA‑GCGCCGCCACGCTATCACt                                                                                                                                                                                          <  1:1178733/239‑1 (MQ=255)                                                      ccTGAGCCAGCGCTGGCGCGTCGTTAATGCCGTCGCCCACCATTGCCACCTGACGTCCTTCACTTTGCAGATGTTTGATCGCTTCGGCTTTACCGTCCGGCAGCACCCCGGCGATCACCTCATCAATCCCTGCTTCTTTGGC‑GATCGCATTGGCGGTGGGTGGGTTATCCCCGGTCAACATCCCCAGACGATATCCCGCTTTATGC‑AGGCGGTGCA‑GCGCCCCCACGCTATCACTACg                                                                                                                                                                                       >  1:825357/1‑238 (MQ=255)                                                       cTGAGCCAGCGCTGGCGCGTCGTTAATGCCGTCGCCCACCATTGCCACCTGACGTCCTTCACTTTGCAGATGTTTGATCGCTTCGGCTTTACCGTCCGGCAGCACCCCGGCGATCACCTCATCAATCCCTGCTTCTTTGGC‑GATCGCATTGGCGGTGGTTGGGTTATCCCCGGTCAACATCACCAGACGATATCCCGCTTTATGC‑AGGCGTTGCA‑GCGCCGCCACGCTATCACTACGCa                                                                                                                                                                                     <  1:782134/239‑1 (MQ=255)                                                         gAGCCAGCGCTGGCGCGTCGTTAATGCCGTCGCCCACCATTGCCACCTGACGTCCTTCACTTTGCAGATGTTTGATCGCTTCGGCTTTACCGTCCGGCAGCACCCCGGCGATCACCTCATCAATCCCTGCTTCTTTGGC‑GATCGCATTGGCGGTGGTTGGGTTATCCCCGGTCAACATCACCAGACGATATCCCGCTTTATGC‑AGGCGTTGCA‑GCGCCGCCACGCTATCACTACGCAAc                                                                                                                                                                                   <  1:900320/239‑1 (MQ=255)                                                             ctgcgcTGGCGCGTCGTTAAGGCCGTCGCCCTCCATTGCCACCTGACGCCCTTCACTTTGCAGATGTGTGATCGCTTCGGCTTTACCGTCCGGCAGCACCCCGGCGATCACCTCATCAATCCCTGCTTCTTTGGC‑GATCGCATTGGCGGTGGTTGGGTTATCCCCGGTCAACATCACCAGACGATATCCCGCTTTATGC‑AGGCGTTGCA‑GCGCCGCCCCGCTATCCCTACGCAACGGAt                                                                                                                                                                               <  2:674617/237‑1 (MQ=255)                                                             cAGCGCTGGCGCGTCGTTAATGCCGTCGCCCACCACTGCCCCCTGACGTCCTTCCCTTTGCAGATGTTTGATCGCTTCGGCTTTACCGTCCGGCAGCACCCCGGCGATCACCTCATCAATCCCTGCTTCTTTGGC‑GATCGCATTGGCGGTGGTTGGGTTATCCCCGGTCAACATCACCAGACGATATCCCGCTTTATGC‑AGGCGTTGCA‑GCGCCGCCACGCTATCACTACGCAACGGAt                                                                                                                                                                               <  2:785985/239‑1 (MQ=255)                                                                 gcTGGCGCGTCGTTAATGCCGTCGCCCACCATTGCCACCTGACGTCCTTCACTTTGCAGATGTTTGATCGCTTCGGCTTTACCGTCCGGCATCACCCCGGCGATCACCTCATCAATCCCTGCTTCTTTGGC‑GATCGCATTGGCGGTGGTTGGGTTATCCCCGGTCAACATCACCAGACGATATCCCGCTTTATGC‑AGGCGTTGCA‑GCGCCGCCACGCTATCACTACGCAACGGATcgcg                                                                                                                                                                           <  1:1122439/239‑1 (MQ=255)                                                                     gcgcGTCGTTAATGCCGTCGCCCACCATTGCCACCTGACGTCCTTCACTTTGCAGATGTTTGATCGCTTCGGCTTTACCGTCCGGCAGCACCCCGGCGATCACCTCATCAATCCCTGCTTCTTTGGC‑GATCGCATTGGCGGTGGTTGGGGTATCCCCGGCCAACATCCCCAAGCCATATCCCGCTTTATGC‑AGGCGTTGCA‑GCGCCCCCACGCTATCACTCAGCACCGGCTCGCGt                                                                                                                                                                          >  2:836577/1‑236 (MQ=255)                                                                     gcgaggcgtTAATGCCCGCGCCCCCCAGTGCCACCTGAAGTCCTTCACTTTGCAGATGTTTGAGCGCTTCGGCTTTACCGTCCGGCAGCACCCCGGCGATCACCTCATCAATCCCTGCTTCTGTGGC‑GATCGCATTGGCGGTGGTTGGGTTATCCCCGGTCATCATCACCAGACGATATCCCGCTTTATGC‑AGGCGTTGCA‑GCGCCGCCACGCTATCACTACGCAACGGATCGCGTAc                                                                                                                                                                        <  2:33376/232‑1 (MQ=255)                                                                           cgtTAATGCCGTCGCCCACCATTGCCACCTGACGTCCTTCACTTTGCAGATGTTTGATCGCTTCGGCTTTACCGTCCGGCAGCACCCCGGCGATCACCTCATCAATCCCTGCTTCTTTGGC‑GATCGCATTGGCGGTGGTTGGGTTATCCCCGGTCAACATCACCAGACGATATCCCGCTTTATGC‑AGGCGTTGCA‑GCGCCGCCACGCTATCACTACGCAACGGATCGCGTACTGCcag                                                                                                                                                                  <  1:1102677/238‑1 (MQ=255)                                                                                  gCCGTCGCCCACCATTGCCACCTGACGTCCTTCACTTTGCAGATGTTTGATCGCTTCGGCTTTACCGTCCGGCAGCACCCCGGCGATCACCTCATCAATCCCTGCTTCTTTGGC‑GATCGCATTGGCGGTGGTTGGGTTATCCCCGGTCAACATCACCAGACGATATCCCGCTTTATGC‑AGGCGTTGCA‑GCGCCGCCACGCTATCACTACGCAACGGATCGCGTACTGCCAGCAGGGCTa                                                                                                                                                          <  1:1193631/239‑1 (MQ=255)                                                                                                ttGCCCCCGCACGTCCTTCACTGTGCAGAGGTTGGCTCGCTTCGGCTTTCCCTCCCGGCAGCCCCCCGGGGATCCCCTCATCAACCCCTGCTCCTTTGGC‑GATCGCATGGGCGGTGGTTGGGTTATCCCCGGTCAACACCCCCAGAGGATACCCCGCTTTATGC‑GGGCGTTGCA‑GCGCCGCCCCGCTTTCCCTACGCACCGGATCGCGTACTGCCAGCAGGGCTACCGCTTCCCCGtaa                                                                                                                                            <  2:73957/239‑3 (MQ=255)                                                                                                       cTGACGTCCTTCACTTTGCAGATGTTTGATCGCTTCGGCTTTACCGTCCGGCAGCACCCCGGCGATCACCTCATCAATCCCTGCTTCTTTGGC‑GATCGCATTGGCGGTGGTTGGGTTATCCCCGGTCAACATCACCAGACGATATCCCGCTTTATGA‑AGGCGGTGCA‑GCGCCGCCACGCTATCACTACGCAAAGGATCGCGTACTGCCAGCAGGGCTACCGCTTTCCCGTCAACCGCca                                                                                                                                     >  1:1081253/1‑239 (MQ=255)                                                                                                          aCGTCCTTCACTTTGCAGATGTTTGATCGCTTCGGCTTTACCGTCCGGCAGCACCCCGGCGATCACCTCATCAATCCCTGCTTCTTTGGC‑GATCGCATTGGCGGTGGTTTGGTTATCCCCGGTCAACATCACCAGACCATATCCCCCTTTATGC‑AGGCGTTTCA‑GCGCCGCCACGCACTCCATATCCCACGGAACGTCGACTGCCCACCGGGGTACCCCATTCCCGGCAAACGCccgc                                                                                                                                   >  2:59012/1‑238 (MQ=255)                                                                                                                       tGCAGATGTTTGATCGCTTCGGCTTTACCGTCCGGCAGCACCCCGGCGATCACCTCATCAATCCCTGCTTCTTTGGC‑GATCGCATTGGCGGTGGTTGGGTTATTCCCGGTCAACATCCCCAGACGAAATCCCGCTTTATGC‑AGGGGTTGCA‑GCGCCCCCACCCCATCACTACGCAACGGAACCGGTACTGCCCGCAGGGCCTACGCCTTCCCCCCCACCCCCAACAGCCCAgggcttg                                                                                                                      >  2:1136380/1‑233 (MQ=255)                                                                                                                               tttGATCGCTTCGGCTTTACCGTCCGGCAGCACCCCGGCGATCACCTCATCAATCCCTGCTTCTTTGGC‑GATCGCATTGGCGGTGGTTGGGGTATCCCCGGTCAACATCACCAGACGATATCCCGCTTTATGC‑AGGCGTTGCA‑GCGCCGCCACGCTATCACTACGCAACGGATCGCGTACTGCCAGCAGGGCTACCGCTTTCCCCTCAACCGCCAGCAGCGCAGGCGT‑TCCCCCTTGCg                                                                                                            >  1:46821/1‑239 (MQ=255)                                                                                                                               tttGATCGCTTCGGCTTCACCGTCCGGCAGCACCCGGGCGACCCCCTCATCAATCCCTGCTTCTTGGGC‑GATCGCATTGGCGGTGGTTGGGTTATCCCCGGTCAACATCCCCAGACGATATCCCGCTTTATGC‑AGGCGTTGCA‑GCGCCGCCACGCTATCACTACGCAACGGATCGCGTACTGCCAGCAGGGCTACCGCTTTCCCGTCAACCGCCAGCAGCACAGGCg                                                                                                                         <  1:936697/227‑1 (MQ=255)                                                                                                                                 tGATCGCTTCGGCTTTACCGTCCGGCAGCACCCCGGCGATCACCTCATCAATCCCTGCTTCTTTGGC‑GATCGCATTGGCGGTGGTTGGGTTATCCCCGGGCCACATCACCAGACGATATACCGCTTTTTGG‑AGGCGTTGCC‑GCGCCGCCACGCCAACACTACGCCACGGAATGCGTACCGCCCGCAGGGCCACCCCTTTCCCGTACACCGCCAGCAGCACAGGCGG‑TGCCCCTTGc                                                                                                             >  2:364423/1‑236 (MQ=255)                                                                                                                                   aTCGCTTCGTCTTTACCGTCCGGCAGCCCCCCGGCGATCACCTCATCAACCCCTGCTTCTTTGGC‑GAGCGCATGGGCGGTGGTTGGGTTATCCCCGGTCAACACCCCCAGACGATATCCCGCTTTATGC‑AGGCGTTGCA‑GCGCCGCCACGCTATCCCTACGCAACGGATCGCGTACTGCCATCGGGGCTACCGCTTTCCCGTCAACCGCCAGCAGCACAGGCGT‑TGCCCCTTGCGATGc                                                                                                        <  2:347319/239‑1 (MQ=255)                                                                                                                                        ttCGGCTTTACCGTCCGGCAGCACCCCGTCGATCACCTCATCAATCCCTGCTTCTTTTGC‑GATCGCATTGGCGGTGGTTGGGTTAGCCCCGGTTAAATTCCCCAGACCCTATCCCTCTTTATGC‑TGTCTGTCCA‑ACGCCGCCACACTAATACAACGTCACTGCTCCGCAACCGCCAGCAGGGCTGCCCCTTTCCCTCCCACCGCCAGCCGCCCCGGCGTCTGCCCCtctgctgccctg                                                                                                     >  2:740912/1‑227 (MQ=255)                                                                                                                                        ttCGGCTTTACCGTCCGGCAGCACCCCGGCGATCACCTCATCAATCCCTGCTTCTTTGGC‑GATCGCATTGGCGGTGGTTGGGTTATCCCCGGTCAACATCACCAGACGATATCCCGCTTTATGC‑Agg                                                                                                                                                                                                                     <  2:1095572/127‑1 (MQ=255)                                                                                                                                        ttCGGCTTTACCGTCCGGCAGCACCCCGGCGATCACCTCATCAATCCCTGCTTCTTTGGC‑GATCGCATTGGCGGTGGTTGGGTTATCCCCGGTCAACATCACCAGACGATATCCCGCTTTATGC‑Agg                                                                                                                                                                                                                     >  1:1095572/1‑127 (MQ=255)                                                                                                                                        ttCGGCTTTACCGTCCGGCAGCACCCCGGCGATCACCTCATCAATCCCTGCTTCTTTGGC‑GATCGCATTGGCGGTGGTTGGGTTATCCCCGGTCAACATCACCAGACGATATCCCGCTTTATGC‑AGGCGTTGCA‑GCGCCGCCACGCTATCCCTACCCCACGGATCGCGTACTGCCAGCAGGGCTACCGCTTTCCCGTCACCCGCCAGCAGCACAGGCGT‑TGCCCCTTGCCATGCCTGa                                                                                                    >  1:499622/1‑238 (MQ=255)                                                                                                                                         tCGGCTTTACCGTCCGGCAGCACCCCGGCGATCACCTCATCAATCCCTGCTTCTTTGGC‑GATCGCATTGGCGGTGGTTGGGTTATCCCCGGTCAACATTACCAAACGATATCCCGCTTTTTGC‑AGGCGTTCCA‑GCGCCGCCACCCTATCACCACGCAACGGATCGCGCACTTCCAGCAGGGCCACCGCTTTTCCGTCACACGCCCGCAGCACGAGCGG‑TGCCCCCTGcctgcctcttat                                                                                                  >  2:936697/1‑228 (MQ=255)                                                                                                                                                           ggcaCCCCGGCGATCCCCTCATCAACCCCTTCTTCGTTGGC‑GACCTCAGTGGCGGTGTTTGGGTTCACCCCGGTCAACACCACCGACCGATACCCCGCTTAATGC‑AGCCGTGGCG‑CCGCCGCCCCGCTTCCACTCCACAACGGACCGCGTACTGCCAGCGGGGCTACCGCTTTCCGGTCAACCGCCAGCAGCACAGGCGT‑TGCCCCTTGCGATGCCTGAGCAGTAATCTCCGCTTCGAt                                                                                <  2:414130/238‑1 (MQ=255)                                                                                                                                                             caCCCCGGCGATCACCACATCACTCCCTGCTTCTTTGGC‑GATCGCATTGGCGGGGGTTGGGTTTTCCCCCGGCAACATAACCAGCCGATATCCCGCTTTATGC‑AGGCGTTGCA‑GCTCCGCCACACTATCACTACCCAACGAATCACCAACTGCCCCCAGTGCATCCCCTTTACTGCCCAACTCCAGACGCCCACGCGC‑TGCCCCTCGCGCTCTCTGACCAGTAATCTCCGCCTCgtgc                                                                               >  2:725315/1‑235 (MQ=255)                                                                                                                                                                cgcggcggTCCCTTCACCACCCCCTGCTTCTTAGGC‑GTTGGCTTGGGCGGTGGTGGGTTTAGCCCCGCCCAACACCCCCAGACGATACCCCCCTTTACGC‑GGGCGTTGCC‑GCGCCGCCCCGCTATCCCTACCAACCGGACCGCGTACGGCCAGCAGGGCTCCCGCTTTCCCGCCAACCGCCAGCAGCACAGGCGT‑TGCCCCTTGCGATGCCTGAGCAGTAATCTCCGCTTCGATAGCtt                                                                           <  2:369764/237‑1 (MQ=255)                                                                                                                                                                  cGGCGATCACCTCATCAATCCCTGCTTCTTTGGC‑GATCGCATTGGCGGTGGTTGGGTTATCCCCGGTCAACATCACCAGACGATATCCCGCTTTATGC‑AGGCGTTGCA‑GCGCCGCCACGCTATCACTACGCAACGGATCGCGTACTGCCAGCAGGGCTACCGCTTTCCCGTCAACCGCCAGCAGCACAGGCGT‑TGCCCCTTGCGATGCCTGAGCAGTAATCTCCGCTTCGATAGCtttt                                                                         >  1:291936/1‑238 (MQ=255)                                                                                                                                                                       tcgcccTCTTCAACCCCGGCTTTTTTGGC‑GATCGGTTTGGCTGTGTTTGGGTTTTCCCGGGCCAACCTCGCCAGACGATATCCCGCTTTAGGC‑GGGCGTGGCC‑GCGCCGCCCCGCTATCACTCCGCACCGGATCGCGTCCTGCCAGCAGGGCTCCCGCTTTCCCGTCAACCGCCAGCAGCACAGGCGT‑TGCCCCTTGCGATGCCTGAGCAGTAATCTCCGCTTCGATAGCTTTGGTAc                                                                     <  2:37060/234‑1 (MQ=255)                                                                                                                                                                       aTCACCTCATCAATCCCTGCTTCTTTGGC‑GATCGCATTGGCGGTGGTTGGGTTATCCCCGGTCCACATCACCAGACGATATCCCGCTTTATGC‑AGGCGTTGCA‑GCGCCGCCACGCTATCACTACGCAACGGATCGCGTACTGCCAGCAGGGCTACCGCTTTCCCGTCCACCGCCAGCAGCACAGGCGC‑TGCCCCTTGCCGTGCCTGCGCAGTAATCTCCGCTTCGCTAGCTTTGGGAcc                                                                    >  2:994614/1‑239 (MQ=255)                                                                                                                                                                              catcaATCCCTGCTTCTTTGGC‑GATCGCATTGGCGGTGGTTGGGTTATCCCCGGTCAACATCACCAGACGATATCCCGCTTTATGC‑AGGCGTTGCA‑GCGCCGCCACGCTATCACTACGCAACGGATCGCGTACTGCCAGCAGGGCTACCGCTTTCCCGTCAACCGCCAGCAGCACAGGCGT‑TGCCCCTTGCGATGCCTGAGCAGTAATCTCCGCTTCGATAGCTTTGGTACCAACCTGt                                                             >  1:50488/1‑239 (MQ=255)                                                                                                                                                                               gtcaCTCCCTGCTACTTCGCG‑CAGCGCTTGGGCTGGGGTGGGGTTCCCCCCGCACAACACCACCAGCCACTCTCACG‑TTTTTGCGAGGCGTTGCA‑GCGCCGCCACCCTTTCCCTCCCCACCGGATCGCGTACGGCCGGCAGGGCTACCGTTTCCCCGTCAACCGCCAGCAGCACAGGCGT‑TGCCCCTTGCGATGCCTGAGCAGTAATCTCCGCTTCGATAGCTTTGGTACCAACCTGtt                                                            <  2:416955/238‑1 (MQ=255)                                                                                                                                                                               atcacccccTGCTTCTTTGCG‑GTGCGCATTGGCGGTGGTTGGGTTACCCCCGGTCAACCTCCCCAGACGATACCCCGCTTTACGC‑AGCGGTGGCA‑GCGCCGCCACGCTATCACCCCGCACCGGCTCGCGTCCTGCCCGCGGGCCTACCGCTTCCCCTCCAACCGCCAGCAGCACAGGCGT‑TGCCCCTTGCGATGCCTGAGCAGTAATCTCCGCTTCGATAGCTTTGGTACCAACCTGtt                                                            <  2:648085/233‑1 (MQ=255)                                                                                                                                                                                 cactCCCTGCTTCTTTGGC‑GATCGCATTGGCGGTGGTTGGGTTATCCCCGGTCACCATCCCCAGCCGATATCCCCCTTTATGC‑AGGCTTTGCA‑GCGCCGCCCCGCTATCACTACGCACCGGATCGCGTACTGCCAGCAGGGCTACCGCTTTCCCGTCAACCGCCAGCAGCACAGGCGT‑TGCCCCTTGCGATGCCTGAGCAGTAATCTCCGCTTCGATAGCTTTGGTACCAACCTGTTGc                                                          <  1:1133876/236‑1 (MQ=255)                                                                                                                                                                                    tCCCTGCTTCTTTGGC‑GATCGCATTGGCGGTGGTTGGGTTATCCCCGGTCAACATCACCAGACGATATCCCGCTTTATGC‑AGGCGTTGCA‑GCGCCGCCACGCTATCACTACGCAACGGATCGCGTACTGCCAGCAGGGCTACCGCTTTCCCGTCAACCGCCAGCAGCACAGGCGT‑TGCCCCTTGCGATGCCTGAGCAGTAATCTCCGCTTCGATAGCTTTTGTACCAACCTGTTGCTCa                                                       >  1:648085/1‑239 (MQ=255)                                                                                                                                                                                      ccTGCTTCTTTGGC‑GATCGCATTGGCGGTGGTTGGGTTATCCCCGGTCAACATCACCAGACGATATCCCGCTTTATGC‑AGGCGTTGCA‑GCGCCGCCACGCTATCACTACGCAACGGATCGCGTACTTCCAGCAGGGCTACCGCTTTCCCGTCAACCGCCCGCAGCACAGGCGT‑TGCCCCTTGCGATGCCTGAGCAGTAATCTCCGCTTCGATAGCCTTGGTACCAACCTGTTGGTCAtt                                                     >  1:363471/1‑239 (MQ=255)                                                                                                                                                                                      ccTGCTTCTTTGGC‑GATCGCATTGGCGGTGGTTGGGTTATCCCCGGTCAACATCACCAGACGATATCCCGCTTTATGC‑AGGCGTTGCA‑GCGCCGCCACGCTATCACTACGCAACGGATCGCGGACTTCCAGCAGGGCTACCGCTTTCCCGTCAACCGCCCGCAGCACAGGc                                                                                                                          >  1:1207136/1‑171 (MQ=255)                                                                                                                                                                                      ccTGCTTCTTTGGC‑GATCGCATTGGCGGTGGTTGGGTTATCCCCGGTCAACATCACCAGACGATATCCCGCTTTATGC‑AGGCGGTTCA‑GCGCCGCCACGCTATCACTACGCAACGGATCGCGTACTGCCAGCAGGGCTACCGCTTTCCCCTCAACCCCCCGCAGCCCAGGCGT‑TCCCCCTTGCGGTGCCTGAGCAGTAATCCCCCCCTCGATAGCCTTGGTACCAACCCGGTGCTCCtt                                                     >  2:781194/1‑239 (MQ=255)                                                                                                                                                                                         gCTTCTTTGGC‑GTTCGAATTGGCGGTGGTTGGGTTATCCCCTGTCAACATCCCCAGACGATATCCCGCTTTATGC‑AGGCGTTGCA‑CCGCCGCCACGCTATCCCTACGCAACGGATCGCGTACTGCCAGCAGGGCTCCCGCTTTCCCGTCAACCGCCAGCAGCACAGGCGT‑TGCCCCTTGCGATGCCTGAGCAGTAATCTCCGCTTCGATAGCTTTGGTACCAACCTGTTGCTCATTTaa                                                  <  1:1208602/239‑1 (MQ=255)                                                                                                                                                                                         gCTTCTTTGGC‑GATCGCATTGGCGGTGGTTGGGTTATCCCCGGTCAACATCACCAGACGATATCCCGCTTTATGC‑AGGCGTTGCA‑GCGCCGCCACGCTATCACTACGCAACGGATCGCGTACTGCCAGCAGGGCTACCGCTTTCCCGTCAACCGCCAGCAGCACAGGCGT‑TGCCCCTTGCGATGCCTGAGCAGTAATCTCCGCTTCGATAGCTTTGGTACCAACCTGTTGCTCATTTa                                                   >  1:917745/1‑238 (MQ=255)                                                                                                                                                                                               ttGGCGGATCGCATGGGCGTGGGTTGGGTCTCCCCCGGCCACCATC‑CCAGGACACATCCCGGTTTCAGC‑CGGCGTTGGACCCGCCCCCCCCCTATCCCTCCGCACCGGATCGCGTACTGCCAGCCGGGTACCCGCTTCCCCGCAAACCGCCAGCAGCACAGGCGT‑TGCCCCTTGCGATGCCTGAGCAGTAATCTCCGCTTCGATAGCTTTGGTACCAACCTGTTGCTCATTTAACAgcg                                             <  2:883108/239‑1 (MQ=255)                                                                                                                                                                                                      aTCGCATTGGCGGTGGTTGGGTTATCCCCGGTCAACATCACCAGACGATATCCCGCTTTATGC‑AGGCGTTGCA‑GCGCCGCCACGCTATCACTACGCAACGGATCGCGTACTGCCAGCAGGGCTACCGCTTTCCCGGCCACCCCCAGCAGCACAGGGGT‑TGCCCCTTGCGATGCCTGAGCAGGAATCTCCGCTTCGATAGCTTTGGTACCAACCTGTTGCTCATTTAACAGCGCCTGAtt                                      >  1:1032658/1‑239 (MQ=255)                                                                                                                                                                                                        cGCATTGGCGGTGGTTGGGTTATCCCCGGTCAACATCACCAGACGATATCCCGCTTTATGC‑AGGCGTTGCA‑GCGCCGCCACGCTATCACTACGCAACGGATCGCGTACTGCCAGCAGGGCTACCGCTTTCCCGTCAACCGCCAGCAGCACAGGCGT‑TGCCCCTTGCGATGCCTGAGCAGTAATCTCCGCTTCGATAGCTTTGGTACCAACCTGTTGCTCATTTAACAGCACCTGATTg                                     >  1:61685/1‑238 (MQ=255)                                                                                                                                                                                                        cGCATTGGCGGTGGTTGGGTTATCCCCGGTCAACATCACCAGACGATATCCCGCTTTATGC‑AGGCGTTGCA‑GCGCCGCCACGCTATCACTACGCAACGGATCGCGTACTGCCAGCAGGGCTACCGCTTTCCCGTCAACCCCCAGCAGCACAGGCGT‑TGCCCCCTGCGATGCCTGAGCAGTAATCCCCGCTTCGGTAGCTTTGGTACCCACCTGTTGCTCATTTAACAGCGCCCGAGTg                                     >  2:742560/1‑238 (MQ=255)                                                                                                                                                                                                        cGCATTGGCGGCGGGTGGGTTATCCCCGGTCAACATCACCAGACGATATCCCGCTTTATGC‑AGGCGTTGCA‑GCGCCGCCACGCTATCACTACGCAACGGATCGCGTACTGCCAGCAGGGCTACCGCTTTCCCGTCAACCCCCAGCAGCAAAGGCGT‑AGCCCCTTGCGATGCCTGCGCAGCAATCTCCGCTCCGGTAGCTTTGGTACCAACCTGTTGCTCATTTAACAGCGCCTGATTg                                     >  2:443192/1‑238 (MQ=255)                                                                                                                                                                                                         cgatggccGGTGTTTGGGTCACCCCCGGCCACCCCCCCCCGCCGCTACCCCGCTTTGTGC‑GGGCGTCGCA‑CCGCCGCCCCGCTACCCCTCCCCACCGGAGCGCGTCCGGCCGGCGGGGCTCCCGCTTCCCCGTCAACCGCCAGCAGCACAGGCGT‑TGCCCCTTGCGATGCCTGAGCAGTAATCTCCGCTTCGATAGCTTTGGTACCATCCTGTTGCTCATTTAACAGCGCCTGATTGcc                                   <  2:415362/232‑1 (MQ=255)                                                                                                                                                                                                                cGGTGGTTGGGTTATCCCCGGTCAACATCACCAGACGATATCCCGCTTTATGC‑AGGCGTTGCA‑GCGCCGCCACGCTATCACTACGCAACGGATCGCGTACTGCCAGCAGGGCTACCGCTTTCCCGTCAACCGCCAGCAGCACAGGCGT‑TGCCCCTTGCGATGCCTGAGCAGTAATCTCCGCTTCGATAGCTTTGGTACCAACCTGTTGCTCATTTAACAGCGCCTGATTGCCCAGCAAt                            >  1:713930/1‑239 (MQ=255)                                                                                                                                                                                                                 gggggtTGGGTTACCCCCGGTCAACATCCCCAGACGATATCCCGCTTTATGC‑AGGCGTTGCA‑GCGCCGCCCCGCTATCACTACGCAACGGATCGCGTACTGCCAGCAGGGCTACCGCTTTCCCGTCAACCGCCAGCAGCACAGGCGT‑TGCCCCTTGCGATGCCTGAGCAGTAATCTCCGCTTCGATAGCTTTGGTACCAACCTGTTGCTCATTTAACAGCGCCTGATTGCCCAGCAAt                            <  1:808233/235‑1 (MQ=255)                                                                                                                                                                                                                         ggTTATCCCCGGTCAACATCACCAGACGATATCCCGCTTTATGC‑AGGCGTTGCA‑GCGCCGCCACGCTATCACTACGCAACGGATCGCGTACTGCCAGCAGGGCTACCGCTTTCCCGTCAACCGCCAGCAGCACAGGCGT‑TGCCCCTTGCGCTGCCTGAGCAGTAATCTCCGCTTCGCTAGCTTTGGTACCAACCTGTTGCTCATTTAACAGCGCCTGATTGCCCAGCAATAAAGCATg                    >  2:1062436/1‑238 (MQ=255)                                                                                                                                                                                                                         ggTTATCCCCGGTCAACATCACCAGACGATATCCCGCTTTATGC‑AGGCGTTGCA‑GCGCCGCCACGCTATCACTACGCAACGGATCGCGTACTGCCAGCAGGGCTACCGCTTTCCCGTCAACCGCCAGCAGCACAGGCGT‑TGCCCCTTGCGATGCCTGAGCAGTAATCTCCGCTTCGATAGCTTTGGTACCAACCTGTTGCTCATTTAACAGCGCCTGATTGCCCAGCAATAACGCATg                    >  2:1115527/1‑238 (MQ=255)                                                                                                                                                                                                                         ggTTATCCCCGGTCAACATCACCAGACGATATCCCGCTTTATGC‑AGGCGTTGCA‑GCGCCGCCACGCTATCACTACGCAACGGATCGCGTACTGCCAGCAGGGCTACCGCTTTCCCGTCAACCGCCAGCAGCACAGGCGT‑TGCCCCTTGCGATGCCTGAGCAGTAATCTCCGCTTCGATAGCTTTGGTACCAACCTGTTGCTCATTTAACACCGCCTGATTGCCCAGCAATAACGCATg                    >  2:197681/1‑238 (MQ=255)                                                                                                                                                                                                                             aTCCCCGGTCAACATCACCAGACGATATCCCGCTTTATGC‑AGGCGTTGCA‑GCGCCGCCACGCTATCACTACGCAACGGATCGCGTACTGCCAGCAGGGCTACCGCTTTCCCGTCAACCGCCAGCAGCACAGGCGT‑TGCCCCTTGCGATGCCTGAGCAGTAATCTCCGCTTCGATAGCTTTGGTACCAACCTGTTGCTCATTTAACAGCGCCTGATTGCCCAGCAATAACGCATGACCt                >  1:1066391/1‑238 (MQ=255)                                                                                                                                                                                                                             aTCCCCGGTCAACATCACCAGACGATATCCCGCTTTATGC‑AGGCGTTGCA‑GCGCCGCCACGCTATCACTACGCAACGGATCGCGTACTGCCAGCACGGCTACCGCTTTCCCGTCAACCGCCAGCAGCACAGGCGT‑TGCCCCTTGCGATGACTGAGCAGTAATCTCCGCTTCGATAGCTTTGGTACCACCCTGTTGCTCATTTAACAGGCCCGGATTGCCCAGCAATAACGCATGACCt                >  2:741879/1‑238 (MQ=255)                                                                                                                                                                                                                               ccccGGTCAACATCACCAGACGATATCCCGCTTTATGC‑AGGCGTTGCA‑GCGCCGCCACGCTATCACTACGCAACGGATCGCGTACTGCCAGCAGGGCTACCGCTTTCCCGTCAACCGCCAGCAGCACAGGCGT‑TGCCCCTTGCGATGCCTGAGCAGTAATCTCCGCTTCGATAGCTTTGGTACCAACCTGTTGCTCATTTAACAGCGCCTGATTGCCCAGCAATAACGCATGACCTTCa             >  1:748883/1‑239 (MQ=255)                                                                                                                                                                                                                                    gTCAACATCACCAGACGATATCCCGCTTTATGC‑AGGCGTTGCA‑GCGCCGCCACGCTATCACTACGCAACGGATCGCGTACTGCCAGCAGGGCTACCGCTTTCCCGTCAACCGCCAGCAGCACAGGCGT‑TGCCCCTTGCGATGCCTGAGCAGTAATCTCCGCTTCGATAGCTTTGGTACCAACCTGTTGCTCATTTAACAGCGCCTGATTGCCCAGCAATAACGCATGACCTTCAGCTTc        >  1:743688/1‑239 (MQ=255)                                                                                                                                                                                                                                          ctCACCAGACGATACCCCGCTTTACCG‑AGGGGTGGCA‑GCGCCGCCGCCCTATCCCTACGCAACGGATCGCGTACTGCCAGAAGGGCTACCGCTTTCCCGTCAACCGCCAGCAGCACAGGCGT‑TGCCCCTTGCGATGCCTGAGCAGTAATCTCCGCTTCGATAGCTTTGGTACCAACCTGTTGCTCATTTAACAGCGCCTGATTGCCCAGCAATAACGCATGACCTTCAGCTTCACCGCt  <  1:792600/238‑1 (MQ=255)                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                               CGCGGTTTCAATGGCAACATCACTGCCGCCACCCATCGCAATGCCGACATCCGCCTGAGCCAGCGCTGGCGCGTCGTTAATGCCGTCGCCCACCATTGCCACCTGACGTCCTTCACTTTGCAGATGTTTGATCGCTTCGGCTTTACCGTCCGGCAGCACCCCGGCGATCACCTCATCAATCCCTGCTTCTTTGGC‑GATCGCATTGGCGGTGGTTGGGTTATCCCCGGTCAACATCACCAGACGATATCCCGCTTTATGC‑AGGCGTTGCA‑GCGCCGCCACGCTATCACTACGCAACGGATCGCGTACTGCCAGCAGGGCTACCGCTTTCCCGTCAACCGCCAGCAGCACAGGCGT‑TGCCCCTTGCGATGCCTGAGCAGTAATCTCCGCTTCGATAGCTTTGGTACCAACCTGTTGCTCATTTAACAGCGCCTGATTGCCCAGCAATAACGCATGACCTTCAGCTTCACCGCT  >  NC_000913/509136‑509606

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑