Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,977,527 G→T 17.5% *417L (TGA→TTA) 
M1M (ATG→ATT) †
wzxE →
wecF →
O‑antigen translocase
TDP‑Fuc4NAc:lipidIIFuc4NAc transferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,977,5270GT17.5% 159.6 / 2.9 75*417L (TGA→TTA) 
M1M (ATG→ATT) †
wzxE
wecF
O‑antigen translocase
TDP‑Fuc4NAc:lipidIIFuc4NAc transferase
Reads supporting (aligned to +/- strand):  ref base G (34/26);  new base T (12/2);  total (47/28)
Fisher's exact test for biased strand distribution p-value = 6.53e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.44e-01

TAGTGGGTGATGTGTTAAAAGTGGGCGCTTATGTCTTTGGTTATCTGGTGATCGCCAAAGCGTCACTGCGGTTTTATATTCTGGCGGAAGTCAGCCAGTTCACTTTATTGATGGTATTTGCCCACTGGCTAATCCCTGCGCATGGTGCACTGGGCGCGGCGCAGGCATATATGGCAACTTATATCGTCTATTTTTCTCTTTGTTGTGGCGTGTTTTTACTCTGGCGTAGGCGGGCATGACTGTACTGATTCACGTACTGGGATCGGATATCCCTCACCATAACCGAACCGTTTTGCGGTTTTTCAATGACGCGCTGGCCGCGACGAGCGAGCACGCGCGCGAGTTTATGGTTGTTGGCAAGGACGACGGCTTAAGTGATAGCTGTCCGGCGCTTTCTGTGCAATTTTTCCCTGGGAAAAAATCGCTGGCGGAAGCGGTCATCGCGAAAGCAAAAGCTAACCGTCAGCAGCGT  >  NC_000913/3977290‑3977761
                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                
tAGTGGGTGATGTGTTAAAAGTGGGCGCTTATGTCTTTGGTTATCTGGTGATCGCCAAAGCGTCACTGCGGTTTTATATTCTGGCGGAAGTCAGCCAGTTCACTTTATTGATGGTATTTGCCCACTGGCTAATCCCTGCGCATGGTGCACTGGGCGCGGCGCAGGCATATATGGCAACTTATATCGTCTATTTTTCTCTTTGTTGTGGCGTGTTTTTACTCTGGCGTAGGCGGGCATGa                                                                                                                                                                                                                                                 <  2:1086012/239‑1 (MQ=255)
tAGTGGGTGATGTGTTAAAAGTGGGCGCTTATGTCTTTGGTTATCTGGTGATCGCCAAAGCGTCACTGCGGTTTTATATTCTGGCGGAAGTCAGCCAGTTCACTTTATTGATGGTATTTGCCCACTGGCTAATCCCTGCGCATGGTGCACTGGGCGCGGCGCAGGCATATATGGCAACTTATATCGTCTATTTTTCTCTTTGTTGTGGCGTGTTTTTACTCTGGCGTAGGCGGGCATGa                                                                                                                                                                                                                                                 <  1:393903/239‑1 (MQ=255)
      gTGATGTGTTAAAAGTGGGCGCTTATGTCTTTGGTTATCTGGTGATCGCCAAAGCGTCACTGCGGTTTTATATTCTGGCGGAAGTCAGCCAGTTCACTTTATTGATGGTATTTGCCCACTGGCTAATCCCTGCGCATGGTGCACTGGGCGCGGCGCAGGCATATATGGCAACTTATATCGTCTATTTTTCTCTTTGTTGTGGCGTGTTTTTACTCTGGCGTAGGCGGGCATGACTGTa                                                                                                                                                                                                                                            <  1:449253/238‑1 (MQ=255)
      gTGATGTGTTAAAAGGTGGCGCTTATGTCTTTGGTTATCTGGTGATCGCCAAAGCGTCACTGCGGTTTTATATTCTGGCGGAAGTCAGCCAGTTCACTTTATTGATGGTATTTGCCCACTGGCTAATCCCTGCGCATGGTGCACTGGGCGCGGCGCAGGCATATATGGCAACTTATATCGTCTATTTTTCTCTTTGTTGTGGCGTGTTTTTACTCTGGCGTAGGCGGGCATGACTGTa                                                                                                                                                                                                                                            <  2:798082/238‑1 (MQ=255)
         aTGTGTTAAAAGTGGGCGCTTATGTCTTTGGTTATCTGGTGATCGCCAAAGCGTCACTGCGGTTTTATATTCTGGCGGAAGTCAGCCAGTTCACTTTATTGATGGTATTTGCCCACTGGCTAATCCCTGCGCATGGTGCACTGGGCGCGGCGCAGGCATATATGGCAACTTATATCGTCTATTTTTCTCTTTGTTGTGGCGTGTTTTTACTCTGGCGTAGGCGGGCTTGTCTTTatttg                                                                                                                                                                                                                                        >  2:409781/1‑235 (MQ=255)
         aTGTGTTAAAAGTGGGCGCTTATGTCTTTGGTTATCTGGTGATCGCCAAAGCGTCACTGCGGTTTTATATTCTGGCGGAAGTCAGCCAGTTCACTTTATTGATGGTATTTGCCCACTGGCTAATCCCTGCGCATGGTGCACTGGGCGCGGCGCAGGCATATATGGCAACTTATATCGTCTATTTTTCTCTTTGTTGTGGCGTGTTTTTACTCTGGCGTAGGCGGGCATGACTGTACTGa                                                                                                                                                                                                                                        >  1:1130431/1‑239 (MQ=255)
             gtTAAAAGTGGGCGCTTATGTCTTTGGTTATCTGGTGATCGCCAAAGCGTCACTGCGGTTTTATATTCTGGCGGAAGTCAGCCAGTTCACTTTATTGATGGTATTTGCCCACTGGCTAATCCCTGCGCATGGTGCACTGGGCGCGGCGCAGGCATATATGGCAACTTATATCGTCTATTTTTCTCTTTGTTGTGGCGTGTTTTTACTCTGGCGTAGGCGGGCATGACTGTACTGATTCa                                                                                                                                                                                                                                    <  1:103497/239‑1 (MQ=255)
                     tGGGCGCTTATGTCTTTGGTTATCTGGTGATCGCCAAAGCGTCACTGCGGTTTTATATTCTGGCGGAAGTCAGCCAGTTCACTTTATTGATGGTATTTGCCCACTGGCTAATCCCTGCGCATGGTGCACTGGGCGCGGCGCAGGCATATATGGCAACTTATATCGTCTATTTTTCTCTTTGTTGTGGCGTGTTTTTACTCTGGGGTAGGCGGGCCTGTCTGTTCTGTTTCTCGTTCttt                                                                                                                                                                                                                            >  2:508916/1‑237 (MQ=255)
                      gggCGCTTATGTCTTTGGTTATCTGGTGATCGCCAAAGCGTCACTGCGGTTTTATATTCTGGCGGAAGTCAGCCAGTTCACTTTATTGATGGTATTTGCCCACTGGCTAATCCCTGCGCATGGTGCACTGGGCGCGGCGCAGGCATATATGGCAACTTATATCGTCTATTTTTCTCTTTGTTGTGGCGTGTTTTTTCTCTGGCGGTGGCGGGCATGACTGTATTGATTCTCGTACTtgg                                                                                                                                                                                                                           >  2:490307/1‑239 (MQ=255)
                      gggCGCTTATGTCTTTGGTTATCTGGTGATCGCCAAAGCGTCACTGCGGTTTTATATTCTGGCGGAAGTCAGCCAGTTCACTTTATTGATGGTATTTGCCCACTGGCTAATCCCTGCGCATGGTGCACTGGGCGCGGCGCAGGCATATATGGCAACTTATATCGTCTATTTTTCTCTTTGTTGTGGCGTGTTTTTACTCTGGCGTAGGCGGGCATGACTGTATTGATTCACGTAATggg                                                                                                                                                                                                                           >  1:1039004/1‑239 (MQ=255)
                      gggCGCTTATGTCTTTGGTTATCTGGTGATCGCCAAAGCGTCACTGCGGTTTTATATTCTGGCGGAAGTCAGCCAGTTCACTTTATTGATGGTATTTGCCCACTGGCTAATCCCTGCGCATGGTGCACTGGGCGCGGCGCAGGCATATATGGCAACTTATATCGTCTATTTTTCTCTTTGTTGTGGCGTGTTTTTACCCTGGCGGAGGCGGGCATTATTCTTCTGTTTAGCGTTCTggg                                                                                                                                                                                                                           >  2:546340/1‑239 (MQ=255)
                        gcgcTTATGTCTTTGGTTATCTGGTGATCGCCAAAGCGTCACTGCGGTTTTATATTCTGGCGGAAGTCAGCCAGTTCACTTTATTGATGGTATTTGCCCACTGGCTAATCCCTGCGCATGGTGCACTGGGCGCGGCGCAGGCATATATGGCAACTTATATCGTCTATTTTTCTCTTTGTTGTGGCGTGTTTTTACTCTGGCGTAGGCGGGCATGACTGTACTGATTCACGTACTGGGa                                                                                                                                                                                                                          <  1:489067/238‑1 (MQ=255)
                            ttATGTCTTTGGTTATCTGGTGATCGCCAAAGCGTCACTGCGGTTTTATATTCTGGCGGAAGTCAGCCAGTTCACTTTATTGATGGTATTTGCCCACTGGCTAATCCCTGCGCATGGTGCACTGGGCGCGGCGCAGGCATATATGGCAACTTATATCGTCTATTTTTCTCTTTGTTGTGGCGTGTTTTTACTCTGGCGTAGGCGGGCATGa                                                                                                                                                                                                                                                 <  1:71471/211‑1 (MQ=255)
                            ttATGTCTTTGGTTATCTGGTGATCGCCAAAGCGTCACTGCGGTTTTATATTCTGGCGGAAGTCAGCCAGTTCACTTTATTGATGGTATTTGCCCACTGGCTAATCCCTGCGCATGGTGCACTGGGCGCGGCGCAGGCATATATGGCAACTTATATCGTCTATTTTTCTCTTTGTTGTGGCGTGTTTTTACTCTGGCGTAGGCGGGCATGACTGTACTGATTCACGTACTGGGATCGGa                                                                                                                                                                                                                     <  1:283395/239‑1 (MQ=255)
                                   tttGGGTAACTGGTGATCGGCAAATCGTCACTGCGGTTTTATATTCTGGCGGAAGTCAGCCAGGTCACTTTATTGATGGTATTTGCCCACTGGCTAATCCCTGCGCATGGTGCACTGGGCGCGGGGCAGGCATATATGGCAACTTATATCGTCTATTTTTCTCTTTGTTGTGGCGTGTTTTTACTCTGGCGTAGGCGGGCATGACTGTACTGATTCACGTACTGGGATCGGATATCCCt                                                                                                                                                                                                             <  2:527445/239‑1 (MQ=255)
                                      ggTTATCTGGTGATCGCCAAAGCGTCACTGCGGTTTTATATTCTGGCGGAAGTCAGCCAGTTCACTTTATTGATGGTATTTGCCCACTGGCTAATCCCTGCGCATGGTGCACTGGGCGCGGCGCAGGCATATATGGCAACTTATATCGTCTATTTTTCTCTTTGTTGTGGCGTGTTTTTACTCTAGGGCTGGCGAGCATGACTGTACTTTTTTATGTACTGGGGTCGGATATCCCCCa                                                                                                                                                                                                           >  1:273151/1‑238 (MQ=255)
                                             tGGTGATCGCCAAAGCGTCACTGCGGTTTTATATTCTGGCGGAAGTCAGCCAGTTCACTTTATTGATGGTATTTGCCCACTGGCTAATCCCTGCGCATGGTGCACTGGGCGCGGCGCAGGCATATATGGCAACTTATATCGTCTATTTTTCTCTTTGTTGTGGCGTGTTTTTAATCTGGTGTAGTCGGGCATGTCTGTACTGATTCTCGTACTGGGATCGTATAACCCTCACCATaaac                                                                                                                                                                                                   >  2:443689/1‑237 (MQ=255)
                                                             gTCACTGCGGTTTTATATTCTGGCGGAAGTCAGCCAGTTCACTTTATTGATGGTATTTGCCCACTGGCTAATCCCTGCGCATGGTGCACTGGGCGCGGCGCAGGCATATATGGCAACTTATATCGTCTATTTTTCTCTTTGTTGTGGCGTGTTTTTACTCTGGCGTAGGCGGGCAGGACTGTACTGATTCACGTACTGGGATCGGATATCCCTCACCATAACCGAACCGTTTTGCgg                                                                                                                                                                                   <  1:123979/237‑1 (MQ=255)
                                                              tCACTGCGGTTTTATATTCTGGCGGAAGTCAGCCAGTTCACTTTATTGATGGTATTTGCCCACTGGCTAATCCCTGCGCATGGTGCACTGGGCGCGGCGCAGGCATATATGGCAACTTATATCGTCTATTTTTCTCTTTGTTGTGGCGTGTTTTTACTCTGGCGTAGGCGGGCATGACTGTACTGATTCACGTACTGGGATCGGATATCCCTCACCATAACCGAAACGGTTTGGGGttt                                                                                                                                                                                >  1:896576/1‑239 (MQ=255)
                                                                                 tGGCGGAAGTCAGCCAGTTCACTTTATTGATGGTATTTGCCCACTGGCTAATCCCTGCGCATGGTGCACTGGGCGCGGCGCAGGCATATATGGCAACTTATATCGTCTCTTTTTCTCTTTGTTGTGGCGTGTTTTTACTCTGGCGTAGGCGGGCATGACTGTTCTTGTTCACGGACTGGGCTCCGAGATTCCCCACCCTAACCGGACCATTTTGGTGGTTTTTAATTTAcgcctggac                                                                                                                                                              >  2:562856/1‑232 (MQ=255)
                                                                                 tGGCGGAAGTCAGCCAGTTCACTTTATTGATGGTATTTGACCACTGGCTAATCCCTGCGCATGGTGCACTGGGCGCGGCGCAGGCATATATGGCAACTTATATCGTCTATTTTTCTCTTTGTTGTGGCGTGTTTTTAATCTGGCGTAGGCGGGCGTGTCTGTAATTAATCACGTAATGGGATAGGATATCCCCTCACATAACTCCAACGGATTGTGGGTTTTTACTGACGCGCtcccc                                                                                                                                                             >  2:746243/1‑234 (MQ=255)
                                                                                   gCGGAAGTCAGCCAGTTCACTTTATTGATGGTATTTGCCCACTGGCTAATCCCTGCGCATGGTGCACTGGGCGCGGCGCAGGCATATATGGCAACTTATATCGTCTATTTTTCTCTTTGTTGTGGCGTGTTTTTACTCTGGCGTAGGCGGGCATTACTGTACTTATTCACGTACTGGGATCGGGTATCCCTCACCATAACCGAACCGTTTTGCGGTTTTTCAATGACGCGCTGGGcgcg                                                                                                                                                          >  1:389451/1‑239 (MQ=255)
                                                                                   gCGGAAGTCAGCCAGTTCACTTTATTGATGGTATTTGCCCACTGGCTAATCCCTGCGCATGGTGCACTGGGCGCGGCGCAGGCATATATGGCAACTTATATCGTCTATTTTTCTCTTTGTTGTGGCGTGTTTTTACTCTGGCGTAGGCGGGCATGACTGTACTGATTTACGTACTTGGGTCGGATATCCCTCACCATAAACGAACCGTTTTGCGGTTTTTCTATTACGCGCTGGCcgcg                                                                                                                                                          >  1:778568/1‑239 (MQ=255)
                                                                                   gCGGAAGTCAGCCAGTTCACTTTATTGATGGTATTTGCCCACTGGCTAATCCCTGCGCATGGTGCACTGGGCGCGGCGCAGGCATATATGGCAACTTATATCGTCTATTTTTCTCTTTGTTGTGGCGTCTTTTTTCTCTTCCGTTGCGGGGGTTGGCTTTACTGACTTAAGTCCTTGGATCGGGGATCCCCCCCCCTAAACCAACTCTTTTTCCGTTTTATAATCGCGCGCtttcacc                                                                                                                                                           >  2:3861/1‑232 (MQ=255)
                                                                                    cGGAAGTCAGCCAGTTCACTTTATTGATGGTATTTGCCCACTGGCTAATCCCTGCGCATGGTGCACTGGGCGCGGCGCAGGCATATATGGCAACTTATATCGTCTATTTTTCTCTTTGTTGTGGCGTGTTTTTACTCTGGCGTAGGCGGGCATTTCTGTACTGTTTTACGTACTGGGGTCGGATATCCCTCACCCTAACCCAACCGTTTTTCGGTTTTTTAATGACGCGCTGGCcccg                                                                                                                                                          >  1:1065753/1‑238 (MQ=255)
                                                                                                            tGATGGTATTTGCCCACTGGCTAATCCCTGCGCATGGTGCACTGGGCGCGGCGCAGGCATATATGGCAACTTATATCGTCTATTTTTCTCTTTGTTGTGGCGTGTTTTTTATCTGGGGTAGGCGGGCATGACTGTATTTAGTTCCGTTATTGGGTTGGGTATCCCTCACCCTTACCGCACCGTTTTGCGGGTTTTTACTGTCGCGCCTGCCGCGCACAGCGCGCAcgcgcgcggggttt                                                                                                                                 >  2:561123/1‑233 (MQ=255)
                                                                                                            tGATGGTATTTGCCCACTGGCTAATCCCTGCGCATGGTGCACTGGGCGCGGCGCAGGCATATATGGCAACTTATATCGTCTATTTTTCTCTTTGTTGTGGCGTGTTTTTACTCTGGCGTAGGCGGGCATGACTGTACTGATTCACGTACTGGGATCGGATATCCCTCACCATAACCGAACCGTTTTGCGGTTTTTCAATGACGCGCTGGCCGCGACGAGCGAGCACGCGCGCGAGTTTa                                                                                                                                <  1:3861/239‑1 (MQ=255)
                                                                                                               tGGTAATTGCCCACTGGCTAATCCCTGCGCATGGTGCACTGGGCGCGGCGCAGGCATATATGGCAACTTATATCGTCTATTTTTCTCTTTGTTGTGGCGTGTTTTTACTCTGGCGTAGGCGGGCATGACTGTACTGATTCACGTACTGGGATCGGATATCCCTCACCATAACCGAACCGTTTTGCGGTTTTTCAATGACGCGCTGGCCGCGACGAGCGAGCACGCGCGCGAGTTTATgg                                                                                                                             <  1:897591/239‑1 (MQ=255)
                                                                                                                 gTATTTGCCCACTGGCTAATCCCTGCGCATGGTGCACTGGGCGCGGCGCAGGCATATATGGCAACTTATATCGTCTATTTTTCTCTTTGTTGTGGCGTGTTTTTACTCTGGCGTAGGCGGGCATGACTGTACTGATTCACGTACTGGGATCGGATATCCCTCCCCATAACCGAACCGTTTTGCGGTTTTTCAATGGCGCGCTGGCCGCGACGAGCGAGCACCCGCGCGGGTTTTTGgtt                                                                                                                           >  1:401267/1‑239 (MQ=255)
                                                                                                                 gTATTTGCCCACTGGCTAATCCCTGCGCATGGTGCACTGGGCGCGGCGAAGGCATATATGGCAACTTATATCGTCTATTTTTCTCTTTGTTGTGGCGTGTTTTTACTCTGGCGTAAGCGGGCCATACTTTACTTGTTCACGTACTGGGATCGGATATACCTCCACATAAACGAAACGTTTTTGGGTTTTTCAATGACTCGCTGGCCGCGCACCGCCAACTCCCGCCCGAGTTTTTGgtt                                                                                                                           >  2:859220/1‑239 (MQ=255)
                                                                                                                            cTGGCTAATCCCTGCGCATGGTGCACTGGGCGCGGCGCAGGCATATATGGCAACTTATATCGTCTATTTTTCTCTTTGTTGTGGCGTGTTTTTACTCTGGCGTAGGCGGGCATTACTTTACTTATTTACGTACTGGGATTGGATATCCCCTCCCATAACCGAACCGTTTTTCGGTTTTTCAATGGCGCGCTTGCCCCGACCCGCGCGCCCCCGCGCGGGTTTATTGTTGTTGTGAAgg                                                                                                                >  2:608072/1‑238 (MQ=255)
                                                                                                                             tGGCTAATCCCTGCGCATGTTGCACTGGTCGCGTCGCAGGCATATATGGCAACCTATATCGTCTATTTTTCTCTTTGTTGTGTCGTGTTTTTACTCTGGCGTAGGCGGGCATGACTGTACTGATTCACGTACTGGGATCGGATATCCCTCACCATAACCGAACCGTTTTGCGGTTTTTCAATGACGCGCTGGCCGCGACGAGCGAGCACGCGCGCGAGTTTATGGTTGTTGGCAAGgac                                                                                                              <  2:349133/239‑1 (MQ=255)
                                                                                                                             tGGCTAATCCCTGCGCATGGTGCACTGGGCGCGGGGCAGGCATATATGGCAACTTATATCGTCTATTTTTCTCTTTGTTGTGGCGTGTTTTTACTCTGGCGTAGGCGGGCATGACTGTACTGATTCACGTACTGGGATCGGATATCCCTCACCATAACCGAACCGTTTTGCGGTTTTTCAATGACGCGCTGGCCGCGACGAGCGAGCACGCGCGCGAGTTTATGGTTGTTGGCAAGgac                                                                                                              <  1:646768/239‑1 (MQ=255)
                                                                                                                              ggCTAATCCCTGCGCATGGTGCACTGGGCGCGGCGCAGGCATATATGGCAACTTATATCGTCTATTTTTCTCTTTGTTGTGGCGTGTTTTTACTCTGGCGTAGGCGGGCATGACTGTACTGATTCACGTACTGGGATCGGATATCCCTCACCATAACCGAACCGTTTTGCGGTTTTTCAATGACGCGCTGGCCGCGACGAGCGAGCACGCGCGCGAGTTTATGGTTGTTGGCAAGgacg                                                                                                             <  2:1130431/239‑1 (MQ=255)
                                                                                                                                ctgaTCCCGGCGCATGGTGCACTGGGCGCGGCGAAGGCATATATGGCAACTTATATCGTCTATTTTTCTCTTTGTTGTGGCGTGTTTTTACTCTGGCGTAGGCGGGCATGACTGTACTGATTCACGTACTGGGATCGGATATCCCTCACCATAACCGAACCGTTTTGCGGTTTTTCAATGACGCGCTGGCCGCGACGAGCGAGCACGCGCGCGAGTTTATGGTTGTTGGCAAGgacga                                                                                                            <  2:778568/235‑1 (MQ=255)
                                                                                                                                cTAATCCCTGCGCATGGTGCACTGGGCGCGGCGCAGGCATATATGGCAACTTATATCGTCTATTTTTCTCTTTGTTGTGGCGTGTTTTTACTCTGGCGTAGGCGGGCATGACTGTACTGATTCACGTACTGGGATCGGATATcc                                                                                                                                                                                                               <  1:779283/144‑1 (MQ=255)
                                                                                                                                cTAATCCCTGCGCATGGTGCACTGGGCGCGGCGCAGGCATATATGGCAACTTATATCGTCTATTTTTCTCTTTGTTGTGGCGTGTTTTTACTCTGGCGTAGGCGGGCATGACTGTACTGATTCACGTACTGGGATCGGATATCCCTCAACATAACCGAACCGTTTTGCGGTTTTTCAATGACGCGCTGGCCGCGACGAGCGAGCACGCGCGCGAGTTTATGGTTGTTGGCAAGgacga                                                                                                            <  1:720651/238‑1 (MQ=255)
                                                                                                                                    tCCCTGCTCAGGGTGCACTGGGCTCGGCGTAGGCATATATGGCAACTTAGAGCGTCTATTTTTCTCTTTGTTGTAGCGTGTGTTGACGCTGGCGTAGGCGGGCATTACTGTACTGATTCACGTACTGAGATCGGATATCCCTCACCATAAACGAACCGTTTTGCGGTTTGTTAATGACGCGCTGGCCGCGACGAGCGATCACGCTCGCGAGTTTATGGTTGTTGGCAAGGACGACGGCt                                                                                                       <  2:795890/239‑1 (MQ=255)
                                                                                                                                    tCCCTGCGCATGGTGCACTGGGCGCGGCGCAGGCATATATGGCAACTTATATCGTCTATTTTTCTCTTTGTTGTGGCGTGTTTTTACTCTGGCGTAGGCGGGCATGACTGTGCTGGTTCACGTACTGGGATCGGATATCCCTCACCATAACCGAACCGTTTTGCGGTTTTTCAATGACGCGGTGGCCGCGACGGGCGAGCACGCGCGCGAGTTTATGGTTGTTGGCAAGGACGACGGCt                                                                                                       <  1:509747/239‑1 (MQ=255)
                                                                                                                                      ccTGCGCATGGTGCACTGGGCGCGGCGCAGGCATATATGGCAACTTATATCGTCTATTTTTCTCTTTGTTGTGGCGTGTTTTTACTCTGGCGTAGGCGGGCATGACTGTACTGATTCACGTACTGGGATCGGATATCCCTCACCATAACCGAACCGTTTTGCGGTTTTTCAATGACGCGCTGGCCGCGACGAGCGAGCACGCGCGCGAGTTTATGGTTGTTGGCAAGGACGACGGCt                                                                                                       <  2:505470/237‑1 (MQ=255)
                                                                                                                                         gcgcATGGTGCACTGGGCGCGGCGCAGGCATATATGGCAACTTCTATCGTCTATTTTTCTCTTTGTTGTGGCGTGTTTTTACTCTTGCGTAGGCGGGCATGTCTTTTTTGATTCACGTACTGGGATCGGCTATCCCTCCCCCTAACCGCAACGCTTTTCGGCTTTTTAATGACGCTCTGGCCGCGCCACACGAGCACGCGCCCGCGTTTTTTGTTTTTGGCGAGGACGACTGCCTa                                                                                                     >  2:419816/1‑236 (MQ=255)
                                                                                                                                         gcgcATGGTGCACTGGGCGCGGCGCAGGCATATATGGCAACTTATATCGTCTATTTTTCTCTTTGTTGTGGCGTGTTTTTACTCTGGCGTAGGCGGGCATTACTGTACTGATTTTCGTACTGGGATTGGATATCCCTGACCATAACCGACCCGTTTTGCGGGTTTTCAAGGACGCGCCTGACGCGACGCGAGATCACGCGCGCGGGTTTATGGTTGGTGTGCAGGAGGACGGCTTAAgc                                                                                                  >  2:612105/1‑238 (MQ=255)
                                                                                                                                         gcgcATGGTGCACTGGGCGCGGCGCAGGCATATATGGCAACTTATATCGTCTATTTTTCTCTTTGTTGTGGCGTGTTTTTACTCTGGCGTAGGCGGGCATGACTGTACTGATTCACGTACTGGGGTCGGGTATCCCTCACCATAACCGAACCGTTTTTCGGGTTTTTAATGACGCGCTGGCCGCGGCGAGCGAGCACGCGCGCGGGTTTTTGGGTGTTGGCAAGggcggcggccttagg                                                                                                  >  1:836843/1‑233 (MQ=255)
                                                                                                                                          cgcATGGTGCACTGGGCGCGGCGCAGGCATATATGGCAACTTATATCGTCTATTTTTCTCTTTGTTGTGGCGTGTTTTTACTCTGGCGTAGGCGGGCATGACTGTACTGTTTTACGTACTGGGGTCGGGTATCCCTCACCATaa                                                                                                                                                                                                     >  2:779283/1‑144 (MQ=255)
                                                                                                                                               ggTGCACTGGGCGCGGCGCAGGCATATATGGCAACTTATATCGTCTATTTTTCTCTTTGTTGTGGCGTGTTTTTACTCTGGCGTAGGCGGGCCTTACTTTACTGATTCACGGACTGGGGTCGGATATCCCTCACCCAAACCGAACCGTTTTGCGGTTTTTTCATGACGCGCTGGCCGCGACCAGGGGGCACGCGCGCGGGTTTTTTGTTGTTGGGCAGGACGCCGGCGTAAGTggtcg                                                                                             >  2:1032667/1‑234 (MQ=255)
                                                                                                                                                 tGCACTGGGCGCGGCGCAGGCATATATGGCAACTTATATCGTCTATTTTTCTCTTTGTTGTGGCGTGTTTTTACTCTGGCGTAGGCGGGCATGACTGTACTGATTCACGTACTGGGATCGGATATCCCTCACCATAACCGAACCGTTTTGCGGTTTTTCAATGACGCGCTGGCCGCGACGAGCGAGCACGCGCGCGAGTTTATGGTTGTTGGCAAGgac                                                                                                              <  1:532402/219‑1 (MQ=255)
                                                                                                                                                  gCACTGGGCGCGGCGCAGGCATATATGGCAACTTATATCGTCTATTTTTCTCTTTGTTGTGGCGTGTTTTTACTCTGGCGTAGGCGGGCATGACTGTACTGATTCACGTACTGGGATCGGATATCCCTCACCATAACCGAACCGTTTTGCGGTTTTTCAATGACGCGCTGGCCGCGACGAGCGAGCACGCGCGCGAGTTTATGGTTGTTGGCAAGGACGACGGCTTAAGTGATAGCTGt                                                                                         <  1:997598/239‑1 (MQ=255)
                                                                                                                                                      tGGGCGCGGCGCAGGCATATATGGCAACTTATATCGTCTATTTTTCTCTTTGTTGTGGCGTGTTTTTACTCTGGCGTAGGCGGGGCTGACTGTACTGATTCTCGTACTTGGATCGGGTAACCCTCACCATAACCGAACCGTTTTGCGGGTTTTCAATGACGCGCTGGCCGCGACGAGCGAGCCCGCGCGCGGGTTTATGGGTGTTGGCAAGGACGGCGGCGTACGTGATAGCTGGCCgg                                                                                     >  2:1215270/1‑239 (MQ=255)
                                                                                                                                                          cgcggcgcAGGCATATATGGCAACTTATATCGTCTATTTTTCTCTTTGTTGTGGCGTGTTTTTACTCTGGCGGTGGCGGGCATGACTGTACTGATTTACGTTCTGGGATCGGATATACCCCACCATAACCGGACCGTTTTGCGGTTTTTCAATGACGCGCTTGCCGCGACGAGCGAGCCCGCTCGCGGGTTTTTGGTTGGTTGGTAGGGCGCCGGCTTATGTGGTAGCTGTCCGGCGCt                                                                                 >  2:235164/1‑239 (MQ=255)
                                                                                                                                                               cgcAGGCATATATGGCAACTTATATCGTCTATTTTTCTCTTTGTTGTGGCGTGTTTTTACTCTGGCGTAGGCGGGCATGACTGTTCTGTTTCACGTACTGGGGTCGGGTATCCCTCACCCTAACCGAACCGGTTTGTGGGTTTTCTATGACGCGCCTGCCGCGACCAGCGCGCACGCGCGCGCGGTTTTGGGTGTTTGCCAGGGCGGCGGGTTACGTGGACGCTGTgcggcggcttttt                                                                            >  2:29639/1‑232 (MQ=255)
                                                                                                                                                                              cAACTTATATCGTCTATTTTTCTCTTTGTTGTGGCGTGTTTTTTCTCTCGCGTAGGCGGGCATTTGTGTCGTGATTTGCGTACTGTGGTTGGATATAACCTCCCCAAACCCTAACGCTTTTTGGTTTTTTTATGACGCCGCGGCCACTACTAGCGGGCACACCCCCGGGTTTTTGGTTGTTGGGCAGGACGACGGCCTAAGAACTAGCCGTACCGTCTTTTCTTTGCGATTTTTCACTg                                                             >  2:291331/1‑239 (MQ=255)
                                                                                                                                                                              cAACTTATATCGTCTATTTTTCTCTTTGTTGTGGCGTGTTTTTACTCTTGCGTAGGCGGGGATGTCTTTACTGTTTCACGTACTGGGATTGGATATCCCTCCCCATAACCGAACCGTTTTGCGGTTTTTCAATGTCGCGCCTGCCGCGACGAGCGAGCACTCGCGGGGGTTTATGGTTGTTGGTCAGGACGACGGCTTAACGGATAGCTTTCCGGGGCTTTCTGTGCAATTTTTCCCTg                                                             >  2:743224/1‑239 (MQ=255)
                                                                                                                                                                              cAACTTATATCGTCTATTTTTCTCTTTGTTGTGGCGTGTTTTTACTCTGGCGTAGGCGGGGATTACTTTTCTGATTTACGGACTTGGGTCGGGTATTCCTCACCATAACCGGACCGTTTTTCGGGTTTTTAATGACGCGCTGGCCGCGGCCAGCGAGCCACCGCGCGGGTTTATTGTTTTTGGCAAGGGCGGCGGCTTCAGTGGTAGCTGTCCGGCGGTTTTTGTGCACATTTTccccg                                                             >  2:1133342/1‑237 (MQ=255)
                                                                                                                                                                              cAACTTATATCGTCTATTTTTCTCTTTGTTGTGGCGTGTTTTTACTCTGGCGTAGGCGGGCCTGACTGTTCTTATTTACGTTCTTGGATCGGATATTCCTCCCCCTAACCGAACCGGTTTTGCGTTTTTTAATGACGCGCTGGCCCCCACGAGCGCGCACCCGCGCGGGTTTTTGGGTGTTTGGCAGGGAGGCGGGCTAAGTGATAGCTGGTCGGCGCCTTTTGTGCCATTTTTCCCTg                                                             >  2:358287/1‑239 (MQ=255)
                                                                                                                                                                              cAACTTATATCGTCTATTTTTCTCTTTGTTGTGGCGTGTTTTTACTCTGGCGTAGGCGGGCATTACTGTACTGTTTCACGTAATGGGTTCGGATATCCCTCACCTTAACCGAACCGTTTTGCGGGTTTTTAATGACGCGCCGGCCGCGACGAGCGGGCCCGCGCGAGGGTTTTATGTTGTTGGCAAGGGAGACGGACTCCGGGATAGCGGTGCGGGGCCTTTTGTGCTATTTTTCCCTg                                                             >  2:797037/1‑239 (MQ=255)
                                                                                                                                                                              cAACTTATATCGTCTATTTTTCTCTTTGTTGTGGCGTGTTTTTACTCTGGCGTAGGCGGGCATGACTGTAATGTTTTACGTACTTGGGTCGGATATCCCTCACCATAACCGCAACGGTTTTCGGGTTTTTAATGATGCGCTTGCCGCTACCGACGAGCCCGCCCGGGAGTTTTTTGTTTTTGGCAAGGGCGACGGCTTAGGGGATAGCTGTCCGGGGCTTTCTGTGCCATTTTTCCCTg                                                             >  2:892166/1‑239 (MQ=255)
                                                                                                                                                                              cAACTTATATCGTCTATTTTTCTCTTTGTTGTGGCGTGTTTTTACTCTGGCGTAGGCGGGCAAAACTTTATTTAGTCACGTACTGGGATCGGGTATCCCCCACCCAAACCGAACCGTTTTGCGGTTTTTTAATGATGCGCTGGCCGCGACGAACGAACACGCGCGCGGGTTTTTGGTTTTTGGTCAGGATGACGGCATGAGTGATAGCCGTGCGCGGGTTTCTGTGCAATTTTTCCCtc                                                             >  2:1054202/1‑238 (MQ=255)
                                                                                                                                                                              cAACTTATATCGTCTATTTTTCTCTTTGTTGTGGCGTGTATTTTCTCTTGCGAAGGCGGGCATGTCTGTTCTTTTTTCAATGCTGGGGTTGGAGTACCCTCAACATAAACTGACCGTTTTGCGGGTTTTTCATGAAGCGATTGCCGGCAACAGCGAGCGCGCACGCGAGTTTATTGGTGTTGGCGAGGACGTCGGACTAAGTGGTAGCTGTGCGGGGCTTTCTGTGTACttttttactg                                                             >  2:1085485/1‑234 (MQ=255)
                                                                                                                                                                                         gTCTATGTTTCTCTTTGTTGTGGCGTGTTTTTACTCTGGCGTAGGCGGGCATGACTGTACTGATTCACGTACTGGGATCGGATATCCCTCACCATAACCGAACCGTTTTGCGGTTTTTCAATGACGCGCTGGCCGCGACGAGCGAGCACGCGCGCGAGTTTATGGTTGTTGGCAAGGACGACGGCTTAAGTGATAGCTGTCCGGCGCTTTCTGTGCAATTTTTCCCTGGGAAAAAATCg                                                  <  1:546340/239‑1 (MQ=255)
                                                                                                                                                                                          tCTATTTTTCTCTTTGTTGTGGCGTGTTTTTACTCTGGCGTCGGCGGGTATGACTTTACTTATTTACGTTCCGGGGTTGGATTTCCCCCCCCCAAACCGAACCGTTTTGCGGGTTTTCAATGGCGCCCCCGCCGCCGCCGGCGCGAACCCGCTCGGGTTTTTGGTTGTTGGGACGGCCGACGGCTTAAGTGTGAGGTGTCCGGCGCTTTTTTTGCAATTTTTCCCCGGGAAAAAAta                                                   >  2:1113765/1‑236 (MQ=255)
                                                                                                                                                                                          tCTATTTTTCTCTTTGTTGTGGCGTGTTTTTACTCTGGCGTAGCCGGGCATGTCTTTTCTGTTTCTCGTACTGGGATCGGATATCCCTCCCCATTACCGAACCCATTTGCGGGTTTTTAATGGACCGCTCGCCCCCCCGCGCGACGCCCCGCGCGGGCTTTCTGGTGTTTGCAAGGAGCACCGCGTTAGTGGATGCTGTTCGGCGCTTTCTTGGCCAATTTTTCCTCGGcaaaaactg                                                  >  2:161036/1‑235 (MQ=255)
                                                                                                                                                                                            tATTTTTCTCTTTGTTGTGGCGTGTTTTTACTCTGGCGTAGGCGGGCATTAATTTATTGGTTTACGTACTGGGGTCGGATATCCCCCACCATAACCGAAACGTTTTGCGGGTTTTTCATGACCGCCCTGCCGCCACGAGCGAACACCCGCCCCGGTTTTTGGGTGTTTGCATGGGCGCCGGGCTTAGCGGACGCCGTTCCGCGCCTTTTGTGGTATTTTTCCCCGGGGAACAAttcctg                                               >  2:1061760/1‑234 (MQ=255)
                                                                                                                                                                                            tATTTTTCTCTTTGTTGTGGCGTGTTTTTACTCTGGCGTAGGCGGGCATGACTGTACTGATTCACGTACTTGGATCGGATATCCCTCACCATAACCGAACCGTTTTGCGGGTTTTCAATGACGCGCTGGCCGCGACGAGCGAGCCCCCGCGCGAGTTTATGGTTGTTGGCCAGGACGGCGGCGTAAGGGATAGCTGTCCCGCGCTTTTTGTGCAATTTTTTCCTGGGGAAAAATCGCtt                                               >  2:12649/1‑238 (MQ=255)
                                                                                                                                                                                            tATTTTTCTCTTTGTTGTGGCGTGTTTTTAATCTGGCGTAGGCGGGCATTACTGTACTGATTCACGTTCTTGGATCGGGTATTCCTCACCATAACCGAACCGTTTTGCGGTTTTTCAATGACGCGCTGGCCGCGACCGGCGAGCACGCGCGCGGGGTTATGGGTGTTGGGAAGGGCGACGGGCTAAGGGGTAGCTGTCCGGCGCTTTCTGTGCGATTTTTCCCTGGGAAAAAATCGCtc                                               >  2:1016988/1‑238 (MQ=255)
                                                                                                                                                                                                        tgttgtGGCGTGTTTTTACTCTGGCGTAGGCGGGGCTGACTTTACTTTTTTCCGTATTTTGAACGGGTATTCCTCATCATAAACCAACCGTTTTTCGGGTTTTCAATTAAGCGCTTGCCCCGAAGAGCGACGCCACGGGCGAGTTTTTTGTTGTTTGCCAGGAAGGCCGGTTACGACATAGCTGTCCGGCGCTTTCTGTGCAATTTTTTCCTGGGCAAAACAACCTGGCGGAAggcgtt                                   >  2:951209/1‑234 (MQ=255)
                                                                                                                                                                                                        tgttgtGGCGTGTTTTTACTCTGGCGTAGGCGGGCATGACTGTACTGATTTACGTACTGGGGTCGGGTATCCCTCACCATAACCGAAACGTTTTTCGGTTTTTTAATGACGCGCTGGCCGCGACGAGCGAGCACGCGCGCGAGTTTATGGTTGTTGGGAAGGACGACGGCTTAAGTGATAGCTGTCCGGGGCTTTTTGTGGAATTTTTTCCTTGGGAAAAATCGCTGGGGGGAGAgg                                     >  1:95217/1‑237 (MQ=255)
                                                                                                                                                                                                          ttgtGGCGTGTTTTTACTCTGGCGTAGGCGGGCATGACTGTACTGATTCACGTACTGGGATCGGATATCCCTCACCATAACCGAACCGTTTTGCGGTTTTTCAATGACGCGCTGGCCGCGACGAGCGAGCAcgcgcg                                                                                                                                         <  1:214734/137‑1 (MQ=255)
                                                                                                                                                                                                          ttgtGGCGTGTTTTTACTCTGGCGTAGGCGGGCATGACTGTACTGATTCACGTACTGGGATCGGATATCCCTCACCATAACCGAACCGTTTTGCGGTTTTTCAATAACGCGCTGGCCGCGACGATCGAGCACGCGCGCGAGTTTATGGTTGTTGGCAAGGACGACGGCTTAAGTGATAGCTGTCCGGCTCTTTCTGTGCAATTTTTCCCTGGGAAAAAAGCGCTGGCGGAAGCGGTc                                   <  1:165463/237‑1 (MQ=255)
                                                                                                                                                                                                              ggCGTGTTTTTACTCTGGCGTAGGCGGGCATTACTGTACTGATTCACGTACTGGGTTCGGATATCCCTCACCATAACCGAACCTTTTTGCGGTTTTTCAATGACGCGCTGGCCGCGACGAGCGAGCACGCGCGCGAGTTTATGGTTGTTGGCAAGGACGACGGCTTAAGTGATAGCTGTCCGGCGCTTTCTGTGCAATTTTTCCCTGGGAAAAAATCGCTGGCGGAAGCGGTCATcgcg                             <  1:1045464/239‑1 (MQ=255)
                                                                                                                                                                                                                     ttttACTCTGGCGTAGGCGGGCATGACTGTACTGATTCACGTACTGGGATCGGATATCCCTCACCATAACCGAACCGTTTTGCGGTTTTTCAATGACGCGCTGGCCGCGACGAGCGAGCACGCGCGCGAGTTTATGGTTGTTGGCAAGGACGACGGCTTAAGTGATAGCTGTCCGGCGCTTTCTGTGCAATTTTTCCCTGGGAAAAAATCGCTGGCGGAAGCGGTCATCGCGAAAGCa                       <  1:508916/238‑1 (MQ=255)
                                                                                                                                                                                                                            ctGGCGTAGGCGGGCATGACTGTACTGATTCACGTACTGGGATCGGAGATCCCTCACCATAACCGAACCGTTTTGCGGTTTTTCAATGACGCGCTGGCCGCGACGAGCGAGCACGCGCGCGAGTTTATGGTTGTTGGCAAGGACGACGGCTTAAGTGATAGCTGTCCGGCGCTTTCTGTGCAATTTTTCCCTGGGAAAAAATCGCTGGCGGAAGCGGTCATCGCGAAAGCAAAAGCTa                >  2:607122/1‑238 (MQ=255)
                                                                                                                                                                                                                              ggCGTAGGCGGGCATGACTGTACTGATTCACGTACTGGGTTCCGATATCCCTCACCATAACCGTACCGTTTTGCGGCTTTTCAATGACGCGCTGGCCCCGCCGAGCGAGCCCGCGCGCGTGTTTATGGTTGTTCGCAAGGACGACGGTTTTAGTGATAGCTGTCCGGCGCTTTCTGTGCAATTTTTCTCTGGCAATAAATCGCTGGCGGAAGCGGTCATCGCGAAGGCCAAAGATAAcc             >  2:701289/1‑239 (MQ=255)
                                                                                                                                                                                                                                         gCATGACTGTACTGATTCACGTACTGGGATCGGATATCCCTCACCATAACCGAACCGTTTTGCGGTTTTTCAATGACGCGCTGGCCGCGACGAGCGAGCACGCGCGCGAGTTTATGGTTGTTGGCAAGGACGACGGCTTAAGTGATAGCTGTCCGGCGCTTTCTGTGCAATTTTTCCCTGGGAAAAAATCGCTGGCGGAAGCGGTCATCGCGAAAGCAAAAGCTAACCGTCAGCAGCGt  >  1:1024699/1‑239 (MQ=255)
                                                                                                                                                                                                                                         gCATGACTGTACTGATTCACGTACTGGGATCGGATATCCCTCACCATAACCGAACCGTTTTGCGGTTTTTCAATGACGCGCTGGCCGCGACGAGCGAGCACGCGCGCGAGTTTATGGTTGTTGGCAAGGACGACGGCTTAAGTGATAGCTGTCCGGCGCTTTCTGTGCAATTTTTCCCTGGGAAAAAATCGCTGGCGGAAGCGGTCATCGCGAAAGCAAAAGCTAACCGTCAGCAGCGt  >  1:1097093/1‑239 (MQ=255)
                                                                                                                                                                                                                                         gCATGACTGTACTGATTCACGTACTGGGATCGGATATCCCTCACCATAACCGAACCGTTTTGCGGTTTTTCAATGACGCGCTGGCCGCGACGAGCGAGCACGCGCGCGAGTTTATGGTTGTTGGCAAGGACGACGGCTTAAGTGATAGCTGTCCGGCGCTTTCTGTGCAATTTTTCCCTGGGAAAAAATCGCTGGCGGAAGCGGTCATCGCGAAAGCAAAAGCTAACCGTCAGCAGCGt  >  1:1094356/1‑239 (MQ=255)
                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                
TAGTGGGTGATGTGTTAAAAGTGGGCGCTTATGTCTTTGGTTATCTGGTGATCGCCAAAGCGTCACTGCGGTTTTATATTCTGGCGGAAGTCAGCCAGTTCACTTTATTGATGGTATTTGCCCACTGGCTAATCCCTGCGCATGGTGCACTGGGCGCGGCGCAGGCATATATGGCAACTTATATCGTCTATTTTTCTCTTTGTTGTGGCGTGTTTTTACTCTGGCGTAGGCGGGCATGACTGTACTGATTCACGTACTGGGATCGGATATCCCTCACCATAACCGAACCGTTTTGCGGTTTTTCAATGACGCGCTGGCCGCGACGAGCGAGCACGCGCGCGAGTTTATGGTTGTTGGCAAGGACGACGGCTTAAGTGATAGCTGTCCGGCGCTTTCTGTGCAATTTTTCCCTGGGAAAAAATCGCTGGCGGAAGCGGTCATCGCGAAAGCAAAAGCTAACCGTCAGCAGCGT  >  NC_000913/3977290‑3977761

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: