| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 4,145,088 | G→C | 11.4% | R365P (CGC→CCC) | pflD → | putative glycine radical domain‑containing pyruvate formate‑lyase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,145,088 | 0 | G | C | 11.4% | 288.2 / 6.3 | 125 | R365P (CGC→CCC) | pflD | putative glycine radical domain‑containing pyruvate formate‑lyase |
| Reads supporting (aligned to +/- strand): ref base G (65/44); new base C (2/14); total (67/58) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.33e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 138 total aligned reads displayed.
GGGCGAAGATGCGGCGTTCCTGAAAGAACTGCTCGAATCTTTATGGGTGAAATGCAACGACATCGTGCTGTTGCGC‑TCCACCAGTAGCGCGCGTTATTTCGCCGGTTTCCCGA‑CCGGCTATACCGCACTGCTCGGCGGGTTAACCGAGAACGGACGTAGCGCGGTGAAC‑G‑TGCTTTCGTTCCTTTG‑CCT‑TGACGCCTATCAAAGCGTGCAATTACCGCAACCGAACCTCGGCGTGCGCACTAACGCCTTGATCGACACGCCGTTCCTGATGAAAACCGCCGA‑AACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTC‑CTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCGTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGATGGA > NC_000913/4144852‑4145325 | gggCGAAGATGCGGCGTTCCTGAAAGAACTGCTCGAATCTTTATGGGTGAAATGCAACGACCTCGTGCTGTTGCGC‑TCCACCAGTAGCGCGCGTTATTTCGCCGCTTTCCCGA‑CCGGCTATACCGCACTGCTCGGCGGCGTAACCGATAACCGATCTAGCGCGGTGCAC‑C‑TGCTTCCGTACCTTTA‑CCT‑TCACGCCTACCAAAGCGTGCAATTTCCGCAACCGCACCTAGGAGGgcgc > 2:401063/1‑238 (MQ=255)gggCGAAGATGCGGCGTTCCTGAAAGAACTGCTCGAATCTTTATGGGTGAAATGCAACGACATCGTGCTGTTGCGC‑TCCACCAGTAGCGCGCGTTATTTCGCCGGTTTCCCGA‑CCGGCTATACCGCACTGCTCGGCGGGTTAACCGAGAACGGACGTAGCGCGGTGACC‑G‑TGCTTTCGTTCCTTTG‑CCT‑TCACGCCTATCACAGCGTGGCATTCCCGCACCCGAACCCCGGCGTgcgc > 2:852206/1‑238 (MQ=255)gggCGAAGATGCGGCGTTCCTGAAAGAACTGCTCGAATCTTTATGGGTGAAATGCAACGACATCGTGCTGTTGCGC‑TCCACCAGTAGCGCGCGTTATTTCGCCGGTTTCCCGA‑CCGGCTATACCGCACTGCTCGGCGGGTTAACCGAGAACGGACGTAGCGCGGTGAAC‑G‑TGCTTTCGTTCCTTTG‑CCT‑TTACGCCTATCAAAGCGTGCAATTACCGCAACCGAACCTCGGCGTgcgc > 1:15880/1‑238 (MQ=255)gggCGAAGATGCGGCGTTCCTGAAAGAACTGCTCGAATCTTTATGGGTGAAATGCAACGACATCGTGCTGTTGCGC‑TCCACCAGTAGCGCGCGTTATTTCGCCGGTTTCCCGA‑CCGGCTATACCGCACTGCTCGGCGGGTTAACCGAGAACGGACGTAGCGCGGTGAAC‑G‑TGCTTTCGTTCCTTTG‑CCT‑TGACGCCTATCAAAGCGTGCAATTACCGCAACCGAACCTCGGCGTgcgc > 1:76244/1‑238 (MQ=255)gggCGAAGATGCGGCGTTCCTGAAAGAACTGCTCGAATCTTTATGGGTGAAATGCAACGACATCGTGCTGTTGCGC‑TCCACCAGTAGCGCGCGTTATTTCGCCGGTTTCCCGA‑CCGGCTATACCGCACTGCTCGGCGGGTTAACCGAGAACGGACGTAGCGCGGTGAAC‑G‑TGCTTTCGTTCCTTTG‑CCT‑TGACGCCTATCAAAGCGTGCAATTACCGACACCGAACCTCGGCGTgcgc > 1:135082/1‑238 (MQ=255)gggCGAAGATGCGGCGTTCCTGAAAGAACTGCTCGAATCTTTATGGGTGAAATGCAACGACATCGTGCTGTTGCGC‑TCCACCAGTAGCGCGCGTTATTTCGCCGGTTTCCCGA‑CCGGCTATACCGCACTGCTCGGCGGGTTAACCGAGAACGGACGTAGCGCGGTGAAC‑G‑TGCTTTCGTTCCTTTG‑CCT‑TGACGCCTATAACAGCGTGCAATTAACGCAACCGAACCTAGGCGTgagc > 1:106653/1‑238 (MQ=255)gggCGAAGATGCGGCGTTCCTGAAAGAACTGCTCGAATCTTTATGGGTGAAATGCAACGACATCGTGCTGTTGCGC‑TCCACCAGTAGCGCGCGTTATTTCGCCGGTTTCCCGA‑CCGGCTATACCGCACTGCTCGGCGGGTTAACCGAGAACGGACGCAGCGCGGTGAAC‑G‑TGCTTTCGTTCCTTTG‑CCT‑TGACGCCTGTCAAAGCCTGCAATTACGGCAACCGAACCTCCCCGTgcgc > 2:1205975/1‑238 (MQ=255) gCGAAGATGCGGCGTTCCTGAAAGAACTGCTCGAATCTTTATGGGTGAAATGCAACGACATCGTGCTGTTGCGC‑TCCCCCAGTAGCGCGCGTTATTTCGCCGGTTTCCCGA‑CCGGCTATACCGCACTGCTCGGCGGGTTAACCGAGAACGGACGTAGCGCGGTGAAC‑G‑TGCTTTCGTTCCTTTG‑CCT‑TGCCGCCTATCAAAGCGTGCAATTACCGCAACCGAACCTCGGGGTGCGCACt > 1:479167/1‑239 (MQ=255) cGAAGATGCGGCGTTCCTGAAAGAACTGCTCGAATCTTTATGGGTGAAATGCAACGACATCGTGCTGTTGCGC‑TCCACCAGTAGCGCGCGTTATTTCGCCGGTTTCCCGA‑CCGGCTATACCGCACTGCTCGGCGGGTTAACCGAGAACGGACGTAGCGCGGTGAAC‑G‑TGCTTTCGTTCCTTTG‑CCT‑TGACGCCTATCAAAGCGTGCAATTACCGCAACCGAACCTCGGCGTGCGCACt < 2:905314/238‑1 (MQ=255) cGAAGATGCGGCGTTCCTGAAAGAACTGCTCGAATCTTTATGGGTGAAATGCAACGACATCGTGCTGTTGCGC‑TCCACCAGTAGCGCGCGTTATTTCGCCGGTTTCCCGA‑CCGGCTATACCGCACTGCTCGGCGGGTTAACCGAGAACGGACGTAGCGCGGTGAAC‑G‑TGCTTTCGTTCCTTTG‑CCT‑TGACGCCTATCAAAGCGTGCAATTACCGCAACCGAACCTCGGCGTGCGCACt < 1:737940/238‑1 (MQ=255) gAAGATGCGGCGTTCCTGAAAGAACTGCTCGAATCTTTATGGGTGAAATGCAACGACATCGTGCTGTTGCGC‑TCCACCAGTAGCGCGCGTTATTTCGCCGGTTTCCCGA‑CCGGCTATACCGCACTGCTCGGCGGGTTAACCGAGAACGGACGTAGCGCGGTGAAC‑G‑TGCTTTCGTTCCTTTG‑CCT‑TGACGCCTATCAAAGCGTGCAATTACCGCAACCGAACCTCGGCGTGCGCACTa < 1:225424/238‑1 (MQ=255) cggcgTTCCTGAAAGAACTGCTCGAATCTTTATGGGTGAAATGCAACGACATCGTGCTGTTGCGC‑TCCACCAGTAGCGCGCGTTATTTCGCCGGTTTCCCGA‑CCGGCTATACCGCACTGCTCGGCGGGTTAACCGAGAACGGACGTAGCGCGGTGAAC‑G‑TGCTTTCGTTCCTTTG‑CCT‑TGACGCCTATCAAAGCGGGCCATTACCGCAACCGAACCTCGGCGTGCGCACTAACGCCTTg > 1:828852/1‑239 (MQ=255) ccTGAAAGAACTGCTCGAATCTTTATGGGTGAAATGCAACGACATCGTGCTGTTGCGC‑TCCACCAGTAGCGCGCGTTATTTCGCCGGTTTCCCGA‑CCGGCTATACCGCACTGCTCGGCGGGTTAACCGAGAACGGACGTAGCGCGGTGAAC‑G‑TGCTTTCGTTCCTTTG‑CCT‑TGACGCCTATCAAAGCGTGCAATTACCGCAACCGAACCTCGGCGTGCGCACTAACGCCTTGATCGac > 1:255511/1‑238 (MQ=255) aaaGAACTGCTCGAATCTTTATGGGTGAAATGCAACGACATCGTGCTGTTGCGC‑TCCACCAGTAGCGCGCGTTATTTCGCCGGTTTCCCGA‑CCGGCTATACCGCACTGCTCGGCGGGTTAACCGAGAACGGACGTAGCGCGGTGAAC‑G‑TGCTTTCGTTCCTTTG‑CCT‑TGACGCCTATCAAAGCGTGCAATTACCGCAACCGAACCTCGGCGTGCGCACTAACGCCTTGATCGACACGc < 1:852206/238‑1 (MQ=255) aGAACTGCTCGAATCTTTATGGGTGAAATGCAACGACATCGTGCTGTTGCGC‑TCCACCAGTAGCGCGCGTTATTTCGCCGGTTTCCCGA‑CCGGCTATACCGCACTGCTCGGCGGGTTAACCGAGAACGGACGTAGCGCGGTGAAC‑G‑TGCTTTCGTTCCTTTG‑CCT‑TGACGCCTATCAAAGCGTGCAATTACCGCAACCGAACCTCGGCGTGCGCACTAACGCCTTGATCGACACGCCGt > 1:1118699/1‑239 (MQ=255) cTCGAATCTTTATGGGTGAAATGCAACGACATCGTGCTGTTGCGC‑TCCACCAGTAGCGCGCGTTATTTCGCCGGTTTCCCGA‑CCGGCTATACCGCACTGCTCGGCGGGTTAACCGAGAACGGACGTAGCGCGGTGAAC‑G‑TGCTTTCGTTCCTTTG‑CCT‑TGACGCCTATCAAAGCGTGCAATTACCGCAACCGAACCTCGGCGTgcg > 2:261478/1‑206 (MQ=255) cTCGAATCTTTATGGGTGAAATGCAACGACATCGTGCTGTTGCGC‑TCCACCAGTAGCGCGCGTTATTTCGCCGGTTTCCCGA‑CCGGCTATACCGCACTGCTCGGCGGGTTAACCGAGAACGGACGTAGCGCGGTGAAC‑G‑TGCTTTCGTTCCTTTG‑CCT‑TGACGCCTATCAAAGCGTGCAATTACCGCAACCGAACCTCGGCGTGCGCACTAACGCCTTGATCGACACGCCCTTCCtgat > 1:1071362/1‑239 (MQ=255) gTGAAATGCAACGACATCGTGCTGTTGCGC‑TCCACCAGTAGCGCGCGTTATTTCGCCGGTTTCCCGA‑CCGGCTATACCGCACTGCTCGGCGGGTTAACCGAGAACGGACGTAGCGCGGTGAAC‑G‑TGCTTTCGTTCCTTTG‑CCT‑TGACGCCTATCAAAGCGTGCAATTACCGCAACCGAACCTCGGCGTGCGCACTAACGCCTTGATCGACACGCCGTTCCTGATGAAAACCGCCGa‑aa > 1:578290/1‑238 (MQ=255) aaaTGCAACGACATCGTGCTGTTGCGC‑TCCACCAGTAGCGCGCGTTATTTCGCCGGTTTCCCGA‑CCGGCTATACCGCACTGCTCGGCGGGTTAACCGAGAACGGACGTAGCGCGGTGAAC‑G‑TGCTTTCGTTCCTTTG‑CCT‑TGACGCCTATCAAAGCGTGCAATTACCGCAACCGCACCTCGGCGTGCGCCCTAACCCCTTTGTCGACACGCCGTTCCTGATGAAAAACGCCGA‑AAccct > 2:386638/1‑237 (MQ=255) acgctGCGGTTGCGC‑TCCCCCAGGAGCGCGCGTTTTTCCGCCGGTTTCCCGA‑CCGGCTATACCGCGCTGCTCGGCGGGTTAACCGAGACCGGACGGAGCGCGGTAACC‑T‑TGCTTTCGTTCCTTTG‑CCT‑TGACGCCTACCAAAGCGTGCAATTCCCGCACCCGACCCTCGGCGCGCCCACTAACGCTTTGATCGCCACGCGGTTCCTGATGAAAACCGCCGA‑AACCATTCGCTTCGGTAc < 2:270037/235‑1 (MQ=255) accGTGCTGTT‑CGCGTCCCCCAGTGGGGCTCTGTATTCCTCCGCTTCCCCGC‑CCGGGTATCCCGCCCGGCTCGGCGGGTT‑ACCCAAAACCGACCGTGCCGGGTGAACGG‑TACTTTC‑TTCCTTTGCCCT‑TGACCCCAGTCAAAGCGTGAAATTCCCACACCCAAGCCCCGCCGTCCCCACTACCGCATGGTTCGCCACGCCGTTCCTGATGAAAACCGCCGA‑AACCACTCGCTTCGGTAc < 2:779943/237‑1 (MQ=255) aTCGTGCTGTTGCGC‑TCCACCAGTAGCGCGCGTTATTTCGCCGGTTTCCCGA‑CCGGCTATACCGCACTGCTCGGCGGGTTAACCGAGAACGGACGTAGCGCGGTGAAC‑G‑TGCTTTCGTTCCTTTG‑CCT‑TGACGCCTATCAAAGCGTGCAATCACCGCAACCGAACCTCGGCGTGCGCACTAACGCCTTGATCGACCCGCCGATCCTGATGCAAACCGCCGA‑AACCATTCGCTTCGGTa > 1:219539/1‑238 (MQ=255) cGTGCTGTTGCGC‑TCCACCAGTAGCGCGCGTTATTTCGCCGGTTTCCCGA‑CCGGCTATACCGCACTGCTCGGCGGGTTAACCGAGAACGGACGTAGCGCGGTGAAC‑G‑TGCTTTCGTTCCTTTG‑CCT‑TGACGCCTATCAAAGCGTGCAATTACCGCAACCGAACCTCGGCGTGCGCACTAACGCCTTGATCGACACGCCGTTCATGTTGAAAACCGCCGA‑CACCATTCGCTTCGGTACCg > 1:1194119/1‑239 (MQ=255) cGTGCTGTTGCGC‑TCCACCAGTAGCGCGCGTTATTTCGCCGGCTTCCCGA‑CCGGCTATACCGCACTGCTCGGCGGGTTAACCGAGAACGGACGTAGCGCGGTGAAC‑G‑TGCTTTCGTTCCTTTG‑CCT‑TGACGCCTATCAAAGCGGGCAATTACCGCAACCGAACCTCGGCGTGCGCACTAACGCCTTGATCGACACGCCGTTCCTGATGAAAACCGCCGA‑AACCATTACCTTCGGTACCg > 1:923594/1‑239 (MQ=255) tGCTGTTGCGC‑TCCACCAGTAGCGCGCGTTATTTCGCCGGTTTCCCGA‑CCGGCTATACCGCACTGCTCGGCGGGTTAACCGAGAACGGACGTAGCGCGGTGAAC‑G‑TGCTTTCGTTCCTTTG‑CCT‑TGACGCCTATCAAAGCGTGCAATTACCGCAACCGAACCTCGGCGTGCGCACTAACGCCTTGATCGACACGCCGTTCCTGATGAAAACCGCCGA‑AACCATTCGCTTCGGTACCGGt > 1:1077410/1‑239 (MQ=255) caccaGTAGCGCGCGTTATTTCGCCGGTTTCCCGA‑CCGGCTATACCGCACTGCTCGGCGGGTTAACCGAGAACGGACGTAGCGCGGTGAAC‑G‑TGCTTTCGTTCCTTTG‑CCT‑TGACGCCTATCAAAGCGTGCAATTACCGCAACCGAACCTCGGCGTGCGCACTAACGCCTTGATCGACACGCCGTTCCTGATGAAAACCGCCGA‑AACCATTCGCTTCGGTACCGGTATTCCGCAAATCt > 2:288355/1‑239 (MQ=255) ccaGTGGCGCGTGTTTTTTC‑CCGGTTTCCCCACCCGGCTCTCCCGCCCCGCTCGGCGGTTTACCCCAGACCGGACGTAGCGCGGTGAAC‑G‑TGCTTGCGTTCCTTTG‑CCT‑GGACCCCTACAAAAGCTTGCATTTCCCGCAACCGACCCTCGGCGGGCCCACAACCGCTTGTTTCGACACGCCGTTCCTGATGAAAACCGCCGA‑AACCATTCGCTTCGGTACCGGTATTCCGCAAATCttt < 2:1071362/239‑1 (MQ=255) aGCGCGCGTTATTCCGCCGGTTTCCCGA‑CCGGCTATCCCGCACTGCTCGGCGGGTTAACCGAGAACGGACGTAGCGCGGTGAAC‑G‑TGCTTTCGTTCCTTTG‑CCT‑TGACGCCTATCAAAGCGTGCAATTACCGCACCCGACCCTCGGCGTGCGCACTAACGCCTTGATCGACACGCCGTTCCTGATGAAAACCGCCGA‑AACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGa < 1:196885/239‑1 (MQ=255) gcgcgTTATTTCGCCGGTTTCCCGA‑CCGGCTATACCGCACTGCTCGGCGGGTTAACCGAGAACGGACGTAGCGCGGTGAAC‑G‑TGCTTTCGTTCCTTTG‑CCT‑TGACGCCTATCAAAGCGTGCAATTACCGCAACCGAACCTCGGCGTGCGCACTAACGCCTTGATCGACCCGCCGTTCCTGATGAAAACCGCCGA‑AACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGAttg > 1:267655/1‑237 (MQ=255) gcgcgTTATTTCGCCGGTTTCCCGA‑CCGGCTATACCGCACTGCTCGGCGGGTTAACCGAGAACGGACGTAGCGCGGTGAAC‑G‑TGCTTTCGTTCCTTTG‑CCT‑TGACGCCTATCAAAGCGTGCAATTACCGCAACCGAACCTCGGCGTGCGCACTAACGCCTTGATCGACACGCCGTTCCTGATGAAAACCGCCGA‑AACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGa > 1:188933/1‑239 (MQ=255) gcgcgTTATTTCGCCGGTTTCCCGA‑CCGGCTATACCGCACTGCTCGGCGGGTTAACCGAGAACGGACGTAGCGCGGTGAAC‑G‑TGCTTTCGTTCCTTTG‑CCT‑TGACGCCTATCAAAGCGTGCAATTACCGCAACCGAACCTCGGCGTGCGCACTAACGCCTTGATCGACACGCCGATCCTGATGAAAACCGCCGG‑AACCATTCGCGTCGGTACCGGCATTCCGCAAATCTTTAACGCTGa > 1:769894/1‑239 (MQ=255) gcgcgTTATTTCGCCGGTTTCCCGA‑CCGGCTATACCGCACTGCTCGGCGGGTTAACCGAGAACGGACGTAGCGCGGTGAAC‑G‑TGCTTTCGTTCCTTTG‑CCT‑TGACGCCTATCAAAGCGGGCAATTACCGCAACCGAACCTCGGCGTGCGCCCTAACGCCCTTATCGACACCGCGTTCCTAATCAAAACCGCCCACAACCA‑TCGCCTCGGGCCCGGGATTCCGCAAATCTTTAACgggag > 2:134176/1‑235 (MQ=255) gcgcgTTATTTCGCCGGTTTCCCGA‑CCGGCTATACCGCACTGCTCGGCGGGTTAACCGAGAACGGACGTAGCGCGGTGAAC‑G‑TGCTTTCGTTCCTTTG‑CCT‑TGACGCCTACAAAAGCGTGCAATTACCGCAACCGAACCTCGGCGTGCGCAATACCGCCTTGATCGACACGCCGTTCCTGATGAAAACCGCCGA‑AACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATg < 2:356071/238‑1 (MQ=255) gcgcgTTATTTCGCCGGTTTCCCGA‑CCGGCTATACCGCACTGCTCGGCGGGTTAACCGAGAACGGACGGAGCGCGGTGAAA‑G‑CGCTTTCGTTCCTTTG‑CCT‑TGACGCCTAACAAAGCGTGCAATTACCGCAACCGAATCTCGGCGTGCGCACTAAAGCCCTGGTCTACACGCCGTTCCTTGTGAAAACCGCCGA‑AACAATTCTCTCTGCTACCGGTAGTTCGCAAATCTCTAAccttca > 2:49732/1‑234 (MQ=255) gcgcgTTATTTCGCCGGTTTCCCGA‑CCGGCTATACCGCACTGCTCGGCGGGTTAACCGAGAACGGACGCAGCGCGGTGCAC‑G‑TGCGTTCGTTCCTTTG‑CCT‑TGACGCCCTTCAAAGCGTGCAATTACCGCAACCCAACCTCGGCCTGCCCCCTAACACCTTGACCCGTCCGCCCTTCCTGATACAAACCGCCGA‑AACCACTCACTTCCGGACCGCGTTTCCGCAAATCTTTAACGATGa > 2:818354/1‑239 (MQ=255) acgcgTTATTTCGCCGGTTTCCCGA‑CCGGCTATACCGCACTGCTCGGCGGGTTAACCGAGAACGGACGTAGCGCGGTGAAC‑G‑TGCTTTCGTTCCTTTG‑CCT‑TGACGCCTATCAAAGCCTGGAATTACCGCCACCGAAACCCGACAGGCGCACTAACGCCCTGATCGACACGCCGTTCCCGATGAAACCCCCCGC‑AACCATTCACTTCTGTCCCGGCATTCCCCCCATCTTTAACGAtca > 2:171263/2‑237 (MQ=255) cgcgTTATTTCGCCGGTTTCCCGA‑CCGGCTATACCGCACTGCTCGGCGGGTTAACCGAGAACGGACGTAGCGCGGTGAAC‑G‑TGCTTTCGTTCCTTTG‑CCT‑TGACGCCTATCAAAGCGTGCAATTACCGCAACCGAACCTCGGCGTGCGCACTAACGCCTTGATCGACACGCCGTTCCTGATGAAAACCGCCGA‑AACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGaa > 1:165096/1‑239 (MQ=255) cgcgTTATTTCGCCGGTTTCCCGA‑CCGGCTATACCGCACTGCTCGGCGGGTTAACCGAGAACGGACGTAGCGCGGTGAAC‑G‑TGCTTTCGTTCCTTTG‑CCT‑TGACGCCTATCAAAGCGTGCAATTACCGCAACCGAACCTCGGCGTGCGCACTAACGCCTTGATCGACACGCCGTTCCTGATGAAAACCGCCGA‑AACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGaa > 1:661218/1‑239 (MQ=255) cgTTATTTCGCCGGTTTCCCGA‑CCGGCTATACCGCACTGCTCGGCGGGTTAACCGAGAACGGACGTAGCGCGGTGAAC‑G‑TGCTTTCGTTCCTTTG‑CCT‑TGACGCCTATCAAAGCGTGCAATTACCGCAACCGAACCTCGGCGTGCGCACTAACGCCGTGATCGACCAGCCGTTCCTGATGAAAACCGCCGA‑AACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAgt > 1:197373/1‑239 (MQ=255) tATTTCCCGGGTTCCCCGA‑CCGGCTATACCGCACTGCTCGGCGGGTTACCCGAGAACGGACGTAGCGCGGTGAAC‑G‑TGCTTCCGTTCCTTTG‑CCT‑TGACGCCTATCAAAGCGTGAAATTACCGCAACCGAACCCCGGCGTGCGCACTAACGCCTTGATCGACACGCCGTTCCTGATGAAAACCGCCGA‑AACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAgtggt < 2:1094681/239‑1 (MQ=255) tttCCCGA‑CCGGCTATACCCCAGTGCTCGGCGGGTTACCCGAGAACGGACGTAGCGCGGTGAAC‑G‑TGCTTTCTTTCCTTTG‑CCT‑TGACGCCTACAAAAGCGTGCAATTCCCGCAACCGAACCTCGGCGTGCGCACTAACGCCTTGATCGACACGCCGTTCCTGATGAAAACCGCCGA‑AACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCg < 1:958462/238‑1 (MQ=255) ttccctgA‑CGGGCTATCGCTCACTGCTCGGCGGCTCACCTGAGACCGGACGTAGGCGGGTAAAG‑G‑GGCTTTCGTCCCTTTC‑CCT‑AGACGCCTATCAAACGGGGAAAGCCCCGCAACCGACCCGCGCCGGGCGACCGACCGCCTTGATGGACACGCGGTTCCTGAGAAAAACCGCCGA‑AACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCg < 2:1203600/232‑1 (MQ=255) ggCTATACCGCACTGCTCGGCGGGTTAACCGAGAACGGACGTAGAGACGTTAAA‑T‑TGCTTTGGTTCCTTTG‑CCT‑TGACGCCTATCAAAGAGTGCAATTACCGCAACCGAACCTCGGCGGGCGCACTAACGCCATTATCGAACCGCCCTTCCTGATGAAAACCGGCGA‑AACCCATCGCTTCGGCACCGGTAATCCGTAAATCTTTAAAGATGAAGCGGCGGTGCCAGCCTTC‑CTCaaac > 2:1028997/1‑236 (MQ=255) ggCTATAACGCACTGCTCGGCGGGTTAAACGAGAACGGGCGTAGCGCGGAGAAC‑G‑TGCTTTCGTTCCTTTG‑CCT‑TGACGCCTATCAAAGCGAGCAATTACCGCAACCGAACCTCGGCGCGCCCCTTAACGCCTTGAGCGACACGCCGTTCCTGCTGAAAACCGCCGA‑AACCATTCGCTTCGCTACCGGTATTCCTCCAATCATAAACGATGAAGAGGTGGTGCCAGCGTTG‑CTCATCCg > 2:430498/1‑239 (MQ=255) cACTGCTCGGCGGGTTAACCGAGAACGGACGTAGCGCGGTGAAC‑G‑TGCTTTCGTTCCTTTG‑CCT‑TGACGCCTATCAAAGCGTGCAATTACCGCAACCGAACCTCGGCGTGCGCACTAACGCCTTGATCGACACGCCGTTCCTGATGAAAACCGCCGA‑AACCATTCGCTTCGGTACCGGTATTCCGCAAATCtt > 2:284880/1‑193 (MQ=255) aCTGCTCGGCGGGTTAACCGAGAAAGGACGTAGCGCGGTGAAC‑G‑TGCTTTCGTTCCTTTG‑CCT‑TGACGCCTATCAAAGCGTGCAATTACCGCAACCTAACCTCGGCGGGCGCACTAACGCCTTGATCGACACGCCGTTCCTTATGAAAACCGCCGG‑AACCATTCCCTTCGGTACCGGGATTCCGCAAATATTTAACGATGACGTGGTGGTGGCACCGTTC‑CTCTACCGTGTCTTTTc > 2:546463/1‑237 (MQ=255) cTGCTCGGCGGGTTAACCGAGAACGGACGTAGCGCGGTGAAC‑G‑TGCTTTCGTTCCTTTG‑CCT‑TGACGCCTATCAAAGCGTGCAATTACCGCAACCGAACCTCGGCGTGCGCACTAACGCCTTGATCGACACGCCGTTCCTGATGAAAACCGCCGA‑AACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGGGCCAGCGTTC‑CTCAACCGTGGCGTTTc > 1:273520/1‑236 (MQ=255) tGCTCGGCGGGTTAACCGAGAACGGACGTAGCGCGGTGAAC‑G‑TGCTTTCGTTCCTTTG‑CCT‑TGACGCCTATCAAAGCGTGCAATTACCGCAACCGAACCTCGGCGTGCGCACTAACGCCTTGATCGACACGCCGTTCCTGATGAAAACCGCCGA‑AACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGACGTGGTGGTTCCAGCGTTC‑CTCAACCGGGGCGTTTCGCTg > 2:151214/1‑239 (MQ=255) tGCTCGGCGGGTTAACCGAGAACGGACGTAGCGCGGTGAAC‑G‑TGCTTTCGTTCCTTTG‑CCT‑TGACGCCCATCAAAGCGTGCAATTACCGCAACCGAACCTCGGCGTGCGCACTAACGCCTTGATCCACACGCCGTTCCCGATGAAAACCGCCGA‑AACCATTCGCTTCGGTTCCGGTATTCCGCAAATCTTTAAACAAGAAGATGTGGTTCCAGAGCTCACTCAA‑CGAGGCGTTTctctc > 2:1093041/1‑238 (MQ=255) cTCGGCGGGTTAACCGAGAACGGACGTAGCGCGGTGAAC‑G‑TGCTTTCGTTCCTTTT‑CCT‑TGACGCCTATCAAAGCGGGCAATTACCGCAAACGAACCTCGGCGTGCGCACTAACGCCTTGATCGACACGCCGTTCCTGATGAAAACCGCCGA‑AACCCTTCGCTTCGGTACCGGTATTCCGCCAATCTTTAACCATGACATGGCGGTGCCACCGTTC‑CTCCACCCTGGCGTTTCGCttgc > 2:487017/1‑236 (MQ=255) cTCGGCGGGTTAACCGAGAACGGACGTAGCGCGGTGAAC‑G‑TGCTTTCGTTCCTTTG‑CCT‑TGACGCCTATCAAAGCGTGCAATTACCGCAACCGAACCTCGGCGTGCGCACTAACGCCTTGATCGACACGCCGTTCCTGATGGAAACCGCCGA‑AACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGGTGTGCCAGCGTTC‑CCCAACCGTGGCGTTTCGCTGGa > 2:506482/1‑239 (MQ=255) cTCGGCGGGTTAACCGAGAACGGACGTAGCGCGGTGAAC‑G‑TGCTTTCGTTCCTTTG‑CCT‑TGACGCCTATCAAAGCGTGCAATTACCGCAACCGAACCTCGGCGTGCGCACTAACGCCTTGATCGACACGCCGTTCCTGATGAAAACCGCCGA‑AACCATTCGCTTCGGTAACGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTC‑CTCAACCGTGGCGTTTCGCTGGa > 1:1004555/1‑239 (MQ=255) cTCGGCGGGTTAACCGAGAACGGACGTAGCGCGGTGAAC‑G‑TGCTTTCGTTCCTTTG‑CCT‑TGACGCCTACCAAAGCGTGCAATTACCGCAACCGAACCTCTGCGTGCGCACTAACGCCTTGCGCGACCCGCCGTTCCTGATGAAACCCGCCGG‑AACCATTCGCTTCGGTACCGCTAGTCCGCAAAACTTTAACGATGACGTGGTGGCGACAGCGGTC‑GCCAACCGGGGCGTTTCGcgtgg > 2:227456/1‑235 (MQ=255) cggGTTAACCGAGAACGGACGTAGCGCGGTGAAC‑G‑TGCTTTCGTTCCTTTG‑CCT‑TGACGCCTATCAAAGCGTGCAATTACCGCAACCGAACCTCGGCGTGCGCACTAACGCCTTGATCGACACGCCGTTc > 1:789146/1‑130 (MQ=255) gTTAACCGAGAACGGACGTAGCGCGGTGAAC‑G‑TGCTTTCGTTCCTTTG‑CCT‑TGACGCCTATCAAAGCGTGCAATTACCGCAACCGAACCTCGGCGTGCGCACTAACGCCTTGATCGACACGCCGTTCCTGATGAAAACCGCCGA‑AACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTC‑CTCAACCGTGGCGTTTCGCTGGAAGATgcgc > 2:297736/1‑239 (MQ=255) aCCGAGCACGGACGTGGCGCGGTGACC‑G‑GACTTATGTTCCTTTG‑CCT‑GGACGCATATCAATGCGAGCAATTCCCGAAACGGAACCTTGGCGGGCGCACTAACGCCTTGACCGCAACGCCTTTCCTGATGAAAACCGCCGA‑AACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGTCAGCGTTC‑CTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGa < 2:940035/239‑1 (MQ=255) aaCGGACGTAGCGCGGTGAAC‑G‑TGCTTTCGTTCCTTTG‑CCT‑TGACGCCTATCAAAGCGTGCAATTACCGCAACCGAACCTCGGCGTGCGCACTAACGCCTTGATCGACACGCCGTTCCTGATGAAAACCGCCGA‑AACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTC‑CTCAACCGTGGCGTTTCGCTGGAAGATGGGCGCGACTAtt > 1:511738/1‑238 (MQ=255) ccGGCCGGAGCGCGGTGACC‑C‑TGCTTCCGTTCCTTTG‑CTT‑GTCCCCCTCTAAAGGCGTGCAATTACCGCACCCGAACCCGGGCGTGCCCACTAACGCCTTGATCGACACGCCGTTCCTGATGAAAACCGCCGA‑AACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTC‑CTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTcc < 2:941782/238‑1 (MQ=255) aCGGCCGTGGCGGGGT‑AAC‑GTTGCTTTCGTTCCTTCG‑CGT‑TGACCCTTACAAAAGGGTGCAATTCCCGCAACCGACCCTGGGCTTGCCCCCAAACGCCTGGACCGCAACGCCGTTCCTGATAAAAACCGCCGA‑AACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTC‑CTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTAtt < 2:257950/237‑1 (MQ=255) aCGGACGTACCGCGGTAAAC‑T‑TGCTTTCGTTCATTTC‑CCT‑TGCCGCAAATCAAAGCGTGCACTTCCCGCACCCGACCCTGGGCGGGCGCACTAACGCCTTGATCGACACGCCGTTCCTGATGAAAACCGCCGA‑AACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTC‑CTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTAtt < 2:962532/237‑1 (MQ=255) aCGTCGCGGGGTGAAC‑G‑TGCTTTCGTTCCTTGC‑CCT‑TGACCCCTATCAAGGCGTGCAATTGCCGCAACCGAACCCGGGCGTGCGCACTAACGCTTTGATCGACACGCCGTTCCTGATGAAAACCGCCGA‑AACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTC‑CTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCGTa < 1:299791/238‑1 (MQ=255) aCGTAGCGCGGTGAAC‑G‑TGCTTTCGTTCCTTTG‑CCT‑TGACGCCTATCAAAGCGTGCAATTACCGCAACCGAACCTCGGCGTGCGCACTAACGCCTTGACCGACACGCCGTTCCTGATGAAAACCGCCGA‑AACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTC‑CTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCGTa < 1:230425/238‑1 (MQ=255) cgcgcGTTGAAC‑G‑TGTTTTCGTTCCTTTC‑CCT‑TGACGCCTACAAAACCTTGCAATTCCCGCACCCGACCCCCGGCGGGCGCACTACCGCCTTGTTCGACACGCCGTTCCTGAGGAAAACCGCCGA‑AACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTC‑CTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCGTAGTggg < 1:1093041/238‑1 (MQ=255) accgcgTTGAAC‑G‑TTCTTTCGTTCCTTTG‑CCT‑GGACGCCTACCAAAGCGTCCAATTACCGCACCCGACCCCCGGCGTGCGCACTACCGCTTTGATCGACACGCCGTTCCTGATGAAAACCGCCGA‑AACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTC‑CTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCGTAGTggg < 1:660126/237‑1 (MQ=255) aGCGCGGTGACC‑G‑TGCTTTCTTTCCTTGG‑CCT‑TGCCGCCTATCAAAGCGTGCAATTCCCGCAACCGACCCTCGGCGTGCGCACTAACGCCTTGATCGACACGCCGTTCCTGATGAAAACCGCCGA‑AACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTC‑CTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCGTAGTggg < 1:151214/239‑1 (MQ=255) aGCGCGGGGAAC‑T‑TGCTTTCGTCCCTTTG‑CCT‑GGACGCCATACAAGGCGTGCAATTCCCGCACCCGAACCGCGCCGTGCCCACTACCGCCTTGATCGCCACGCCGTTCCTGATAAAAACCGCCGA‑AACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTC‑CTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCGTAGTggg < 2:995037/239‑1 (MQ=255) gcgggttGAAC‑G‑TGCTTTCTTCCCTTTG‑CCT‑TGACCCCTACCAAAGCGTGCATTTCCCGCACCCGACCCCGGGCGTGCGCACTAACCCCTTGATCGACACGCCGTTCCTGATGAAAACCGCCGA‑AACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTC‑CTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCGTAGTggg < 1:284201/232‑1 (MQ=255) gTGAAC‑G‑TGCTTTCGTTCCTTTG‑CCT‑TGACGCCTATCAAAGCGTGCAATTACCGCAACCGAACCTCGGCGTGCGCACTAACGCCTTGATCGACACGCCGTTCCTGATGAAAACCGCCGA‑AACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTC‑CTCAACCGTGGCGTTTCGCTGGAAGATGCGCGAGACTATTCCGTAGTGGGCtgtgt > 1:595365/1‑239 (MQ=255) gTGAAC‑G‑TGCTTTCGTTCCTTTG‑CCT‑TGACGCCTATCAAAGCGTGCAATTACCGCAACCGAACCTCGGCGTGCGCACTAACGCCTTGATCGACACGCCGTTCCTGATGAAAACCGCCGA‑AACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGGGCCAGCGTTC‑CTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCGTAGTGGGCtgtgt > 1:332115/1‑239 (MQ=255) gTGAAC‑G‑TCTTTTCG‑TCCTTTT‑CCTGTGACCCCTACCAAGCGGTGAAATTCCCGCACCCGAGCCTGGGCGTGCCCACTACCGCTTTGATCGACACGCCGTTCCTGATAAAAACCGCCGA‑AACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTC‑CTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCGTAGTGGGCtgtg < 2:76244/238‑1 (MQ=255) c‑G‑TGCTTTCGTTCCTTTG‑CCT‑TGACGCCTATCAAAGCGTGCAATTCCCGCAACCGACCCTCGGCGTGCGCACTAACGCCTTGATCGACACGCCGTTCCTGATGAAAACCGCCGA‑AACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTC‑CTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCGTAGTGGGCTGTGTGGAAt < 1:726489/239‑1 (MQ=255) tttCGTTCCTTTG‑CCT‑TGACGCCTACCAAGGCGGGCAATTCCCGAAACCGACCCTCGGCGTCCGCCTTACCGCTTTGATCGACACGCCGTTCCTGATGAAAACCGCCGA‑AACCATGCGCTGCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTC‑CTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCGTAGTGGGCTGTGTGGAATTATCt < 1:171263/239‑1 (MQ=255) ttCCTTTG‑CCT‑TGACGCCTATCAAAGCGTGCAATTACCGCAACCGAACCTCGGCGTGCGCACTAACGCCTTGATCGACACGCCGTTCCTGATGAAAACCGCCGA‑AACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTC‑ATCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCGTAGTGGGCTGTGTGGCATTATCTCTTc > 1:581999/1‑238 (MQ=255) tgg‑CCT‑TGACGCCATCCAAAGCGCGCATTTCCCGCACCCGAACCGCGGCGTGCCCACTAACGCCTTGAGCGACACCCCGTTCCTGATGAAAACCGCCGA‑AACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTC‑CTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCGTAGTGGGCTGTGTGGAATTATCTATTCCCGGCa < 2:661218/237‑1 (MQ=255) tG‑CCT‑TGACGCCTATCAAAGCGTGCAATTACCGCAACCGAACCTCGGCGTGCGCACTAACGCCTTGATCGACACGCCGTTCCTGATGAAAACCGCCGA‑AACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTC‑CTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCGTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAg > 1:986776/1‑239 (MQ=255) gg‑CCT‑TGCCCCCTTCCAAATGGTGAAATTCCCCCAACCAACCCCCGGCGGCCGCACAAACCCCTGTACCGAAACGCCGTTCCTGATGAAAACCGCCGA‑AACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTC‑CTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCGTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAg < 2:544447/238‑1 (MQ=255) cGCTTACCAAACCGTGCAATTCCCGAACCCGACCCTCGGCGTGCGCACTAACGCCTTGATCGACACGCCGTTCCTGATGAAAACCGCCGA‑AACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTC‑CTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCGTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACg < 1:388270/239‑1 (MQ=255) cGCCTATCAAAGCGTGCAATTCCCGCAACCGACCCTCGGCTTGCCCCCTAACGCCTGGATCGCCACGCCGTTCCTGATGAAAACCGCCGA‑AACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTC‑CTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCGTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACg < 1:1009671/239‑1 (MQ=255) ccTATCAAAGCGTGCACTTCCCGCAACCGACCCTCGGCGTCCGCCCTAACGCCTTGATCGACACCCCGTTCCTGATGAAAACCGCCGA‑AACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTC‑CTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCGTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGc < 1:1205975/239‑1 (MQ=255) tATCAAAGCGTGCAATTACCGCAACCGAACCTCGGCGTGCGCACTAACGCCTTGATCGACACGCCGTTCCTGATGAAAACCGCCGA‑AACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTC‑CTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCGTAGTggg > 1:255111/1‑200 (MQ=255) cAAAGCGTGCAATTACCGCACCCGAACCCCGGCGTCCGCACTAACGCCTTGACCGACACGCCGTTCCTTATGAAAACCGCCGA‑AACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTC‑CTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCGTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCa < 1:150863/239‑1 (MQ=255) cAAAGCGTGAATTTCCCGCACCCAACCCCCGGCGTGCGCTCTAACGCTTTGATCGACACGCCTTTCCTAATGAAAACCGCCGA‑AACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTC‑CTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCGTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCa < 1:920573/239‑1 (MQ=255) aaGCGTGCAATTACCGCAACCGAACCTCGGCGTGCGCACTAACGCCTTGATCGACACGCCGTTCCTGATGAAAACCGCCGA‑AACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTC‑CTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCGTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTg > 2:21199/1‑235 (MQ=255) aGCGTGCAATTACCGCAACCGAACCTCGGCGTGCGCACTAACGCCTTGATCGACACGCCGTTCCTGATGAAAACCGCCGA‑AACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTC‑CTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCGTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATg > 2:1040009/1‑238 (MQ=255) gTGCAATTACCGCAACCGAACCTCGGCGTGCGCACTAACGCCTTGATCGACACGCCGTTCCTGATGAAAACCGCCGA‑AACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTC‑CTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCGTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACa > 1:401393/1‑238 (MQ=255) tGCAATTACCGCAACCGAACCTCGGCGTGCGCACTAACGCCTTGATCGACACGCCGTTCCTGATGAAAACCGCCGA‑AACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTC‑CTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCGTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATc > 1:397366/1‑239 (MQ=255) tGCAATTACCGCAACCGAACCTCGGCGTGCGCACTAACGCCTTGATCGACACGCCGTTCCTGATGAAAACCGCCGA‑AACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTC‑CTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCGTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATc > 1:1010628/1‑239 (MQ=255) tGCAATTACCGCAACCGAACCTCGGCGTGCGCACTAACGCCTTGATCGACACGCCGTTCCTGATGAAAACCGCCGA‑AACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGGGCCAGCGTTC‑CTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCGTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGGATGACATc > 1:965147/1‑239 (MQ=255) gCAATACCCGCACCCGACCCTGGGCGTCGGCACTAACGCCTGGACCGCCACGCCGTTCCTGATGAAAACCGCCGA‑AACCATGCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTC‑CTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCGTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATcg < 1:676418/239‑1 (MQ=255) ctactACCGCCACCACACCTCGTCGTCCCCACGAACGCTTTGAGCGCATCGCCGTCCCTGAAGAAAACCGCCGA‑AACCATTCGCTTCGGTACCGGTATTCCGCAAATCTGTAACGATGAAGTGGTGGTGCCAGCGTTC‑CTCAACCGTGGGGTTTCGCTGTAAGATGCGCGCGACTATTCCGTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGACACTACGGCTTGCATGACATcgc < 2:267655/235‑1 (MQ=255) aatgcccGCAACCGAACCTCGGCGTTCGCACTAACGCCTTGACCGACACGCCGTTCCTGATGAAAACCGCCGA‑AACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTC‑CTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCGTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATcgc < 1:265141/233‑1 (MQ=255) aaTTACCGCAACCGAACCTCGGCGTGCGCACTAACGCCTTGATCGACACGCCGTTCCTGATGAAAACCGCCGA‑AACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTC‑CTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCGTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATcgcg < 1:288355/239‑1 (MQ=255) ccGCAACCGAACCTCGGCGTGCGCACTAACGCCTTGATCGACACGCCGTTCCTGATGAAAACCGCCGA‑AACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTC‑CTCAACCGTGGCGTTTCGCTGGACGATGCGCGCGACTATTCCGTAGTGGGCTGTGGGGACTTATCTATTCCCGGCCGAACCTACGGCTTGGCAGACATTGCGATggt > 2:1172715/1‑237 (MQ=255) cccccGCACCCCGGCGTGCCCCCAAACCCTTTGACCGACACGCCGTCCCAGATGAAAACCGCCGA‑AACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTC‑CTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCGTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTaa < 2:140776/236‑1 (MQ=255) cAACCGAACCTCGGCGTGCGCACTAACGCCTTGATCGACACGCCGTTCCTGATGAAAACCGCCGA‑AACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTC‑CTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGCATATTCCGTAGTGGGCTGTGTGGAATTATCTATTCACGCCAGAACCTACGGCTTGCATGACATACGGATTTTTaa > 2:861683/1‑239 (MQ=255) aaCCTCGGCGTGCGCACTAACGCCTTGATCGACACGCCGTTCCTGATGAAAACCGCCGA‑AACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGGGCCAGCGTTC‑CTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCGTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCCTGACATCGCGATGTTTAATct > 1:268723/1‑236 (MQ=255) ccTCGGCGTGCGCACTAACGCCTTGATCGACACGCCGTTCCTGATGAAAACCGCCGA‑AACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTC‑CTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCGTAGTGGGCTGGGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGa > 2:814671/1‑239 (MQ=255) tggcgcCCTAACCCCTTGGCCGCGACGCCGGTCCTGATGAAAACCGCCGA‑AACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTC‑CTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCGTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGAt < 2:332115/237‑1 (MQ=255) gTGCGCCCCAACGCTGTGACCGACACGCCGTTCCTGATGAAAACCGCCGA‑AACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTC‑CTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCGTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGAt < 2:818582/239‑1 (MQ=255) cgcACTAACGCCTTGATCGACACGCCGTTCCTGATGAAAACCGCCGA‑AACCATTCGCTTTGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTC‑CTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCGTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCCATGTTTAATCTGCTGAAAGTGATGGa > 1:984047/1‑239 (MQ=255) | GGGCGAAGATGCGGCGTTCCTGAAAGAACTGCTCGAATCTTTATGGGTGAAATGCAACGACATCGTGCTGTTGCGC‑TCCACCAGTAGCGCGCGTTATTTCGCCGGTTTCCCGA‑CCGGCTATACCGCACTGCTCGGCGGGTTAACCGAGAACGGACGTAGCGCGGTGAAC‑G‑TGCTTTCGTTCCTTTG‑CCT‑TGACGCCTATCAAAGCGTGCAATTACCGCAACCGAACCTCGGCGTGCGCACTAACGCCTTGATCGACACGCCGTTCCTGATGAAAACCGCCGA‑AACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTC‑CTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCGTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGATGGA > NC_000913/4144852‑4145325 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |