Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	630,153	T→G	17.3%	V87G (GTG→GGG)	cstA →	carbon starvation protein involved in peptide utilization; APC peptide transporter family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	630,153	0	T	G	17.3%	230.2 / 4.1	96	V87G (GTG→GGG)	cstA	carbon starvation protein involved in peptide utilization; APC peptide transporter family protein
Reads supporting (aligned to +/- strand):  ref base T (40/35);  new base G (17/3);  total (58/38)
Fisher's exact test for biased strand distribution p-value = 1.08e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TCGTCTGGACAGTGCTCTCTGTAATGGGAGCATTTGCTCTGGGATACATTGCTTTAAATCGTGGGGAACAGATCAACGCGCTGTGGATTGTGGTGGCGTCGGTCTGTATCTATCTGATCGCTTACCGTTTTTATGGGCTGTATATCGCCAAAAATGTGCTGGCGGTTGACCCGACGCGTATGACGCCAGCGGTGCGCCATAACGACGGGCTGGACTAT‑GTGCCGACGGACAAGAAAGTGCTGTTCGGTCA‑CCATTTTGCGGCCATTGCCGGAG‑CAGGTCCGCTGGTGGGGCCGGTAC‑TGGCGGCGCAAATGGGCTACCT‑GCCGGGGATGATCTGGCTGCTCGCTGGGGTGGTTCTCG‑CCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGCTCGCTGGGTGAGCTGGTCAAAGAAGAGATGGGGCCAACCGCCGGGGTGATTGCGCTGGTGGC  >  NC_000913/629916‑630390                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                   tCGTCTGGACAGTGCTCTCTGTAATGGGAGCATTTGCTCTGGGATACATTGCTTTAAATCGTGGGGAACAGATCAACGCGCTGTGGATTGTGGTGGCGTCGGTCTGTATCTATCTGATCGCTTACCGTTTTTATGGGCTGTATATCGCCAAAAATGTGCTGGCGGTTGACCCGACGCGTATGACGCCAGCGGTGCGCCATAACGACGGGCTGGACTAT‑GTGCCGACGGACAAGAAAGTg                                                                                                                                                                                                                                                   <  1:169115/239‑1 (MQ=255)  cGTCTGGACAGTGCTCTCTGTAATGGGAGCATTTGCTCTGGGATACATTGCTTTAAATCGTGGGGAACAGATCAACGCGCTGTGGATTGTGGTGGCGTCGGTCTGTATCTATCTGATCGCTTACCGTTTTTATGGGCTGTATATCGCCAAAAATGTGCTGGCGGTTGACCCGACGCGCATGACGCCAGCGGTGCGCCATAACGCCGGGCTGGACTAT‑GTGCCGCCGGACAAGAAAGGGc                                                                                                                                                                                                                                                  >  1:340854/1‑239 (MQ=255)             tGCTCTCTGTAATGGGAGCATTTGCTCTGGGATACATTGCTTTAAATCGTGGGGAACAGATCAACGCGCTGTGGATTGTGGTGGCGTCGGTCTGTATCTATCTGATCGCTTACCGTTTTTATGGGCTGTATATCGCCAAAAATGTGCTGGCGGTTGACCCGGCGCGTATGACGCCAGCGGTGCGCCATAACGCCGGGCTGGACTCT‑GGGCCGACGGGCAAGACAGTGCTGCTCGGTCa                                                                                                                                                                                                                                        >  2:1206989/1‑238 (MQ=255)             tGCTCTCTGTAATGGGAGCATTTGCTCTGGGATACATTGCTTTAAATCGTGGGGAACAGATCAACGCGCTGTGGATTGTGGTGGCGTCGGTCTGTATCTATCTGATCGCTTACCGTTTTTATGGGCTGTATATCGCCAAAAATGTGCTGGCGGTTGACCCGACGCGTATGACGCCAGCGGTGCGCCATAACGACGGGCTGGACTAT‑GTGCCGACGGGCAAGAAAGTGCTGTTCGGTCa                                                                                                                                                                                                                                        >  1:1174184/1‑238 (MQ=255)             tGCTCTCTGTAATGGGAGCATTTGCTCTGGGATACATTGCTTTAAATCGTGGGGAACAGATCAACGCGCTGTGGATTGTGGTGGCGTCGGTCTGTATCTATCTGATCGCTTACCGTTTTTATGGGCTGTATATCGCCAAAAATGTGCTGGCGGTTGACCCGACGCGTATGACGCCAGCGGTGCGCCATAACGACGGGCTGGACTAT‑GTGCCGACGGCAAAGAAAGGGCTGTTCGGTCa                                                                                                                                                                                                                                        >  1:96096/1‑238 (MQ=255)               ctctctGTAATGGGAGCATTTGCTCTGGGATACATTGCTTTAAATCGTGGGGAACAGATCAACGCGCTGTGGATTGTGGTGGCGTCGGTCTGTATCTATCTGATCGCTTACCGTTTTTATGGGCTGTATATCGCCAAAAATGTGCTGGCGGTTGACCCGACGCGTATGACGCCAGCGGTGCGCCATAACGACTGGCTGGACTAT‑GGGCCGACGGACAAGAAAGGGCTGTTCGGGCA‑CCa                                                                                                                                                                                                                                    >  1:1092750/1‑239 (MQ=255)               ctctctGTAATGGGAGCATTTGCTCTGGGATACATTGCTTTAAATCGTGGGGAACAGATCAACGCGCTGTGGATTGTGGTGGCGTCGGTCTGTATCTATCTGATCGCTTACCGTTTTTATGGGCTGTATATCGCCAAAAATGTGCTGGCGGTTGACCCGACGCGTATGACGCCAGCGGTGCGCCATAACGACGGGCTGGACTAT‑GTGCCGACGGACAAGAAAGTGCTGTTCGGTCA‑CCa                                                                                                                                                                                                                                    >  1:1160199/1‑239 (MQ=255)               ctctctGTAATGGGAGCATTTGCTCTGGGATACATTGCTTTAAATCGTGGGGAACAGATCAACGCGCTGTGGATTGTGGTGGCGTCGCTCTGTATCTATCTGATCGCTTACCGTTTTTATGGGCTGTATATCGCCAAAAATGTGCTGGCGGTTGACCCGACCCGTATGACGCCCGCGGTGCGCCCTAACGACCGGCTGGGCTAT‑GTTGCGACGGAAAAGGAAGGGCTAGTCGGACA‑CCa                                                                                                                                                                                                                                    >  1:717538/1‑239 (MQ=255)                  tctGTAATGGGAGCATTTGCTCTGGGATACATTGCTTTAAATCGTGGGGAACAGATCAACGCGCTGTGGATTGTGGTGGCGTCGGTCTGTATCTATCTGATCGCTTACCGTTTTTATGGGCTGTATATCGCCAAAAATGTGCTGGCGGTTGACCCGACGCGTATGACGCCCGCGGGGCGCCATAACGACGGGCTGGACTAT‑GTGCCGGCGGACAAGAAAGGGCTGTTCGGTCC‑CCAttt                                                                                                                                                                                                                                 >  1:1138208/1‑239 (MQ=255)                    tGTAATGGGAGCATTTGCTCTGGGATACATTGCTTTAAATCGTGGGGAACAGATCAACGCGCTGTGGATTGTGGTGGCGTCGGTCTGTATCTATCTGATCGCTTACCGTTTTTATGGGCTGTATATCGCCAAAAATGTGCTGGCGGTTGACCCGACGCGTATGACGCCAGCGGTGCGCCATAACGACGGGCTGGACTAT‑GTGCCGACGGACAAGAAAGTGCTGTTCGGTCA‑CCATTTTg                                                                                                                                                                                                                               <  1:914684/239‑1 (MQ=255)                         tGGGAGCATTTGCTCTGGGATACATTGCTTTAAATCGTGGGGAACAGATCAACGCGCTGTGGATTGTGGTGGCGTCGGTCTGTATCTATCTGATCGCTTACCGTTTTTATGGGCTGTATATCGCCAAAAATGTGCTGGCGGTTGACCCGCCCGGTATGACGCCCGCGGTGAGCCAGAACGACGGGGTGGACTAT‑GTGCCGACCGACACGAAAGTGCGGTTAGGTGA‑CCATTTTGCGGc                                                                                                                                                                                                                           >  2:946912/1‑238 (MQ=255)                         tGGGAGCATTTGCTCTGGGATACATTGCTTTAAATCGTGGGGAACAGATCAACGCGCTGTGGATTGTGGTGGCGTCGGTCTGTATCTATCTGATCGCTTACCGTTTTTATGGGCTGTATATCGCCAAAAATGTGCTGGCGGTTGACCCGAAGCGTATGTCGCCAGCCGGGCGCCATAACGCCGGGCTGGACTATGGGGCCGA‑GGACAAGGACGAGCCGTTTGGTCA‑CCATTTTggggg                                                                                                                                                                                                                           >  2:986262/1‑237 (MQ=255)                            gAGCATTTGCTCTGGGATACATTGCTTTAAATCGTGGGGAACAGATCAACGCGCTGTGGATTGTGGTGGCGTCGGTCTGTATCTATCTGATCGCTTACCGTTTTTATGGGCTGTATATCGCCAAAAATGTGCTGGCGGTTGACCCGACGCGTATGACGCCAGCGGGGGGCCCTAACGACGGGGTGGACTAT‑GTGCCGCCGGGCAAGGAAGTGCTGTTCGGTCA‑CCATTTTGCGGCCCtt                                                                                                                                                                                                                       >  1:19882/1‑239 (MQ=255)                            gAGCATTTGCTCTGGGATACATTGCTTTAAATCGTGGGGAACAGATCAACGCGCTGTGGATTGTGGTGGCGTCGGTCTGTATCTATCTGAGCGCTTACCGTTTTTATGGGCTGTATATCGCCAAAAATGTGCTGGCGGTTGACGCGTCGCGGTGGACGCCCGCGGATGGCCATAACGACCGGCTGGCATCA‑GTGGCGCCCAGCAAGAAAGGGATGTTCGGTCA‑CCACTTTGCGGcgcgt                                                                                                                                                                                                                       >  2:671773/1‑235 (MQ=255)                              gCATTTGCTCTGGGATACATTGCTTTAAATCGTGGGGAACAGATCAACGCGCTGTGGATTGTGGTGGCGTCGGTCTGTATCTATCTGATCGCTTACCGTTTTTATGGGCTGTATATCGCCAAGAATGTGCTGGCGGTTGACCCGACGCGTATGGCGCCAGCCGTGCGCCATAACCAGGGGGTGGACTCT‑GTGCCGACGGGCAAGAAAGGGCTGTTGGGTAACCCATTTTgggcccttc                                                                                                                                                                                                                       >  2:80552/1‑230 (MQ=255)                              gCATTTGCTCTGGGATACATTGCTTTAAATCGTGGGGAACAGATCAACGCGCTGTGGATTGTGGTGGCGTCGGTCTGTATCTATCTGATCGCTTACCGTTTTTATGGGCTGTATATCGCCAAAAATGTGCTGGCGGTTGGCCCGCCGCGGATGGCCCCTGCCGGGGGCCATAACGACGGGCTGGACCAG‑GTGCCGACGGACCAGGAAGGGCTGTGCGGGCG‑CCCTTTTGCGGCCATTg                                                                                                                                                                                                                      >  2:502554/1‑238 (MQ=255)                              gCATTTGCTCTGGGATACATTGCTTTAAATCGTGGGGAACAGATCAACGCGCTGTGGATTGTGGTGGCGTCGGTCTGTATCTATCTGATCGCTTACCGTTTTTATGGGCTGTATATCGCCAAAAATGTGCTGGCGGTTGGCCCGAACCGTATGACGCCAGCGGGTGGCACTAATGGCGGGCTGGGAAAT‑GTGCCGACGGGCCAGAAAGGGCTGTTCGGCCA‑ACATTTTGCGGGCATTg                                                                                                                                                                                                                      >  2:894421/1‑238 (MQ=255)                              gCATTTGCTCTGGGATACATTGCTTTAAATCGTGGGGAACAGATCAACGCGCTGTGGATTGTGGTGGCGTCGGTCTGTATCTATCTGATCGCTTACCGTTTTTATGGGCTGTATATCGCCAAAAATGTGCTGGCGGTTGACCCGACGCGTATGACGCCAGCGGTGCGCCATAACGACGGGCTGGACTAT‑GTGCCGACGGACAAGAAAGTGCTGTTCGGTCA‑CCATTTTGCGGCCATTg                                                                                                                                                                                                                      >  1:446132/1‑238 (MQ=255)                              gCATTTGCTCTGGGATACATTGCTTTAAATCGTGGGGAACAGATCAACGCGCTGTGGATTGTGGTGGCGTCGGTCTGTATCTATCTGATCGCTTACCGTTTTTATGGGCTGTATATCGCCAAAAATGTGCTGGCGGTTGACCCGACGCGTATGACGCCAGCGGTGCGCCATAACGACGGGCTGGACTAT‑GTGCCGACGGACAAGAAAGTGCTGTTCGGTCA‑CCATTTTGCGGCCATTg                                                                                                                                                                                                                      >  1:407776/1‑238 (MQ=255)                                aTTTGCTCTGGGATCCATTGCTTTAAATCGTGGGGAACAGATCAACGCGCTGTGGATTGTGGTGGCGTCGGTCTGTATCTATCTGATCGCTTACCGTTTTTATGGGCTGTATATCGCCAAAAATGTGCTGGCGGTTGACCCGACGCGTATGCCGCCAGCGGTGCGCCATAACGACGGGCTGGACTCT‑GTGCCGCCGGACACGAAAGTGCTTTTCCGTCA‑CCATTTTTCCGGCATTGTCg                                                                                                                                                                                                                   >  1:627239/1‑239 (MQ=255)                                aTTTGCTCTGGGATACATTGCTTTAAATCGTGGGGAACAGATCAACGCGCTGTGGATTGTGGTGGCGTCGGTCTGTATCTATCTGATCGCTTACCGTTTTTATGGGCTGTATATCGCCAAAAATGTGCTGGCGGATGACCCGACGCGTATGACGCCAGCGGTGCGCCATAACGACGGGCTGGAATAT‑GTGCCGACGGACAAGAAAGTGCTGTTCGGTCA‑CCATTTTGCGGCCATTGCCg                                                                                                                                                                                                                   >  1:629765/1‑239 (MQ=255)                                aTTTGCTCTGGGATACATTGCTTTAAATCGTGGGGAACAGATCAACGCGCTGTGGATTGTGGTGGCGTCGGTCTGTATCTATCTGATCGCTTACCGTTTTTATGGGCTGTATATCGCCAAAAATGAGCTGGCGGTTGACCCGACGCGTATGACGCCAGCGGTGCGCCATAACGACGGGCTGGACTAT‑GTGCCGACGGACAAGAAAGTGCTGTTCGGTCA‑CCATTTTGCGGCCATTGCCg                                                                                                                                                                                                                   >  1:1068206/1‑239 (MQ=255)                                 tttGCTCTGGGATACATTGCTTTAAATCGTGGGGAACAGATCAACGCGCTGTGGATTGTGGTGGCGTCGGTCTGTATCTATCTGATCGCTTACCGTTTTTATGGGCTGTATATCGCCAAAAATGTGCTGGCGGTTGGCCCGACGCGTATGGCGCCACCGGTGCGCCACAACGGCGGGCGCGGCTCT‑GCGCCGCCGGGCCAGGAAGGGCTCTTTGGGCT‑CCAGTTTGCGGCGCATGCCgg                                                                                                                                                                                                                  >  2:1144208/1‑239 (MQ=255)                                            aTACATTGCTTTAAATCGTGGGGAACAGATCAACGCGCTGTGGATTGTGGTGGCGTCGGTCTGTATCTATCTGATCGCTTACCGTTTTTATGGGCTGTATATCGCCAAGAATGTGCTGGCGGTTGACCCGACGCGTATGACGCCAGCGGTGCGCCATAACGACGGGCTGGACTAT‑GTGCCGACGGACAAGAAAGTGCTGTTCGGTCA‑CCATTTTGCGGCCATTGCCGGAG‑CAGGTCCg                                                                                                                                                                                                       <  1:80552/238‑1 (MQ=255)                                                aTTGCTTTAAATCGTGGGGAACAGATCAACGCGCTGTGGATTGTGGTGGCGTCGGTCTGTATCTATCTGATCGCTTACCGTTTTTATGGGCTGTATATCGCCAAAAATGTGCTGGCGGTTGACCCGACGCGTATGACGCCAGCGGTGCGCCATAACGACGGGCTGGACTAT‑GTGCCGACGGACAAGAAAGTGCTGTTCGGTCA‑CCATTTTGCGGCCATTGCCGGAG‑CAGGTCCGCtggt                                                                                                                                                                                                  <  1:703813/239‑1 (MQ=255)                                                    cTTTAAATCGTGGGGAACAGATCAACGCGCTGTGGATTGTGGTGGCGTCGGTCTGTATCTATCTGATCGCTTACCGTTTTTATGGGCTGTATATCGCCAAAAATGTGCTGGCGGATGACCCGACGCGTATGACGCCTGCGGGGCCCCCTAACGACGGGCTTGGCTAT‑GTGGCGCCGGCCGACCAACGGCTGCTTGGGAA‑CCCTTTTTCGGCCCATGCCGGAG‑GCGGATCGGTGGTgggt                                                                                                                                                                                              >  2:720983/1‑238 (MQ=255)                                                    cTTTAAATCGTGGGGAACAGATCAACGCGCTGTGGATTATGGTGGCGTCGGTCTGTATCTATCTGATCGCTTACCGTTTTTATGGGCTGTATATCGCCAAAAATGTGCTGGCGGTTGACCCGACGCGTATGACGCCAGCGGTGCGCCATAACGCCGGGCTGGACTAT‑GTGCCGACGGACAAGAAAGTGCTGTTCGGTCA‑CCATTTTGCGGCCATTGCCGGGG‑CAGGTCCGCTGGTggg                                                                                                                                                                                               >  1:616945/1‑238 (MQ=255)                                                     tttAAATCGTGGGGAACAGATCAACGCGCTGTGGATTGTGGTGGCGTCGGTCTGTATCTATCTGATCGCTTACCGTTTTTATGGGCTGTATATCGCCAAAAATGTGCTGGCGGTTGACCCGACGCGTATGACGCCAGCGGTGCGCCATAACGACGGGCTGGACTAT‑GTGCCGACGGACAAGAAAGTGCTGTTCGGTCA‑CCATTTTGCGGCCATTGCCGGAG‑CAGGTCCGCTGGTgggg                                                                                                                                                                                              <  2:462043/238‑1 (MQ=255)                                                           tCGTGGGGAACAGATCAACGCGCTGTGGATTGTGGTGGCGTCGGTCTGTATCTATCTGATCGCTTACCGTTTTTATGGGCTGTATATCGCCAAAAATGTGCTGGCGGTTGACCCGACGCGTATGACGCCAGCGGTGCGCCATAACGACGGGCTGGACTAT‑GTGCCGACGGACAAGAAAGTGCTGTTCGGTCA‑CCATTTTGCGGCCATTGCCGGAG‑c                                                                                                                                                                                                              <  1:1121570/216‑1 (MQ=255)                                                              tGGGGAACAGATCAACGCGCTGTGGATTGTGGTGGCGTCGGTCTGTATCTATCTGATCGCTTACCGTTTTTATGGGCTGTATATCGCCAAAAATGTGCTGGCGGTTGGCCCTTCCGGGATGGAGGCAGCGGTGCGCCCTATCGGGGGGCTGGGATAG‑GGGCCGACGGCAACGGAAAGGCCGTTCGGTGC‑CCATTTTGAGTCCATTGGCGGCGACAGGCGCG‑TGGTGGGGGCGGTAT‑Tgg                                                                                                                                                                                   >  2:576434/1‑239 (MQ=255)                                                              tGGGGAACAGATCAACGCGCTGTGGATTGTGGTGGCGTCGGTCTGTATCTATCTGATCGCTTACCGTTTTTATGGGCTGTATATCGCCAAAAATGTGCTGGCGGTTGACCCGGCGCGGATGACGCCAGCGGGGCGCCATAACGACGGGCTGGACTAT‑GTGCCGACGGACAAGAAAGGGCTGTTCGGTCA‑CCATTTTGCGGCCCTTGCCGGGG‑CAGGGCCGCGGGGGGGGCCGCGAC‑Tgg                                                                                                                                                                                   >  2:204631/1‑239 (MQ=255)                                                              tGGGGAACAGATCAACGCGCTGTGGATTGTGGTGGCGTCGGTCTGTATCTATCTGATCGCTTACCGTTTTTATGGGCTGTATATCGCCAAAAATGTGCTGGCGGTTGACCCGACGCGTATGACGCCAGCGGGGCGCCATAACGACGGGCTGGACTAT‑GTGCCGACGGACAAGAAAGTGCTGTTCGGTCA‑CCATTTTGCGGCCATTGCCGGAG‑CAGGTCCGCTGGGGGGGGCGGTAC‑Tgg                                                                                                                                                                                   >  1:1138783/1‑239 (MQ=255)                                                                      aGATCAACGCGCTGTGGATTGTGGTGGCGTCGGTCTGTATCTATCTGATCGCTTACCGTTTTTATGGGCTGTATATCGCCAAAAATGTGCTGGCGGTTGACCCGACGCGTATGACGCCAGCGGTGCGCCATAACGACGGGCTGGACTAT‑GTGCCGACGGACAAGAAAGTGCTGTTCGGTCA‑CCATTTTGCGGCCATTGCCGGAG‑CAGGTCCGCTGGTGGGGCCGGTAC‑TGGCGGCGCaa                                                                                                                                                                           <  2:703390/239‑1 (MQ=255)                                                                           aaCGCGCTGTGGATTGTGGTGGCGTCGGTCTGTATCTATCTGATCGCTTACCGTTTTTATGGGCTGTATATCGCCAAAAATGTGCTGGCGGTTGACCCGACGCGTATGACGCCAGCGGTGCGCCATAACGACGGGCTGGACTAT‑GTGCCGACGGACAAGAAAGTGCTGTTCGGTCA‑CCATTTTGCGGCCATTGCCGGAG‑CAGGTCCGCTGGTGGGGCCGGTAC‑TGGCGGCGCAAATggg                                                                                                                                                                      <  2:370534/239‑1 (MQ=255)                                                                                gcTGTGGATTGTGGTGGCGTCGGTCTGTATCTATCTGATCGCTTACCGTTTTTATGGGCTGTATATCGCCAAAAATGTGCTGGCGGTTTGCCCGACGCGTATGACGCCAGCGGGGCGCCATAACGCCGGGCTGGACTAT‑GTGCCGACGGACCAGGAAGGGCCGTTCGGGCA‑CCATTTTGCGGCCAATGCCGGGG‑CCGGTCCGGCGGGGGGGCCGGGCCTTGTCGGCGCCAATggggac                                                                                                                                                                   >  2:433455/1‑235 (MQ=255)                                                                                  tgtgGATTGTGGTGGCGTCGGTCTGTATCTATCTGATCGCTTACCGTTTTTATGGGCTGTATATCGCCAAAAATGTGCTGGCGGTTGACCCGACGCGTATGACGCCAGCGGTGCGCCATAACGACGGGCTGGACTAT‑GTGCCGACGGACAAGAAAGTGCTGTTCGGTCA‑CCATTTTGCGGCCATTGCCGGAG‑CAGGTCCGCTGGGGGGGCCGGTAC‑GTGCGGCGCCAATGGGCTACCt                                                                                                                                                                >  1:855302/1‑238 (MQ=255)                                                                                  tgtgGATTGTGGTGGCGTCGGTCTGTATCTATCTGATCGCTTACCGTTTTTATGGGCTGTATATCGCCAAAAATGTGCTGGCGGTTGACCCGACGCGTATGACGCCAGCGGTGCGCCATAACGACGGGCTGGAATAT‑GTGCCGACGGACAAGAAAGTGCTGTTCGGTCA‑CCATTTTGCGGCCATTGCCGGAG‑CAGGTCCGCTGGGGGGGCCGGGAC‑TGGCGGCGCAAAAGGGCTACCt                                                                                                                                                                >  1:21617/1‑238 (MQ=255)                                                                                   gtgGATTGTGGTGGCGTCGGTCTGTATCTATCTGATCGCTTACCGTTTTTATGGGCTGTATATCGCCAAAAATGTGCTGGCGGTTGACCCGACGCGTATGACGCCAGCGGTGCGCCATAACGACGGGCTGGACTAT‑GTGCCGACGGACAAGAAAGTGCTGTTCGGTCA‑CCATTTTGCGGCCATTGCCGGAG‑CAGGTCCGCTGGTGGGGCCGGTAC‑TGGCGGCGCAAATGGGCTACCT‑Gc                                                                                                                                                             <  1:422685/239‑1 (MQ=255)                                                                                    tgGATTGTGGTGGCGTCGGTCTGTATCTATCTGATCGCTTACCGTTTTTATGGGCTGTATATCGCCAAAAATGTGCTGGCGGTTGACCCGACGCGTATGACGCCAGCGGTGCGCCATAACGACGGGCTGGACTAT‑GTGCCGACGGACAAGAAAGTGCTGTTCGGTCA‑CCATTTTGCGGCCATTGCCGGAG‑CAGGTCCGCTGGTGGGGCCGGTAC‑TGGCGGCGCTAATGGGCTACCT‑Gcc                                                                                                                                                            >  1:969417/1‑239 (MQ=255)                                                                                       aTTGTGGTGGCGTCGGTCTGTATCTATCTGATCGCTTACCGTTTTTATGGGCTGTATATCGCCAAGAATGTGCTGGCGGTTGACCCGACGCGTATGACGCCAGCGGTGCGCCATAACGACGGGCTGGACTAT‑GTGCCGACGGACAAGAAAGTGCTGTTCGGTCA‑CCATTTTGCGGCCATTGCCGGAG‑CAGGTCCGCTGGGGGGGCCGGTAC‑TGGCGGGGCAAAAGGGCTACCT‑GCCggg                                                                                                                                                         >  1:524906/1‑239 (MQ=255)                                                                                           tggtggCGTCGGTCTGTATCTATCTGATCGCTTACCGTTTTTATGGGCTGTATATCGCCAAAAATGTGCTGGCGGTTGACCCGACGCGTATGAAGCCCGCGGGGCGCCATAACGCCGGGCTGGACTAT‑GTGCCGGCGGACAAGAAAGGGCGGTTTGGTCA‑CCCTTTTGCGGCCCTTGCCGGGG‑ACGGGCCCCTGGGGGGGCCGGGAT‑TGGCGGCGCCCAAGGGGCACCTGGCCgggggt                                                                                                                                                      >  2:1030646/1‑237 (MQ=255)                                                                                              tggCGTCGGTCTGTATCTATCTGATCGCTTACCGTTTTTATGGGCTGTATATCGCCAAAAATGTGCTGGCGGTTGACCCGACGCGTATGACGCCCGCGGTGCGCCATAACGACGGGCTGGACTAT‑GTGCCGAAGGACAAGAAAGTGCTGTTCGGTCA‑CCATTTTGCGGCCATTGCCGGAG‑CAGGTCCGCTGGTGGGGCCGGTAC‑TGGCGGCGCAAATGGGCTACCT‑GCCGGGggtga                                                                                                                                                    >  1:423536/1‑237 (MQ=255)                                                                                                    cgttcgttatctatcGTATCGCTTACCGTTTTTAGGGGCTGTATATCGCCAAAAAGGTGCTGGCGGTTGACCCGACGCGTATGACGCCAGCGGTGCGCCATAACGACGGGCTGGACTAT‑GTGCCGACGGACAAGAAAGTGCTGTTCGGTCA‑CCATTTTGCGGCCATTGCCGGAG‑CAGGTCCGCTGGTGGGGCCGGTAC‑TGGCGGCGCAAATGGGCTACCT‑GCCGGGGATGATCTGgct                                                                                                                                             <  2:941029/231‑1 (MQ=255)                                                                                                    cGGTCTGTATCTATCTGATCGCTTACCGTTTTTATGGGCTGTATATCGCCAAAAATGTGCTGGCGGTTGACCCGACGCGTATGACGCCAGCGGTGCGCCATAACGACGGGCTGGACTAT‑GTGCCGACGGACAAGAAAGTGCTGTTCGGTCA‑CCATTTTGCGGCCATTGCCGGAG‑CAGGTCCGCTGGTGGGGCCGGTAC‑TGGCGGCGCAAATGGGCTGCCT‑GCCGGGGATGATCTGgctg                                                                                                                                            <  1:331004/239‑1 (MQ=255)                                                                                                    cGGTCTGTATCTATCTGATCGCTTACCGTTTTTATGGGCTGTATATCGCCAAAAATGTGCTGGCGGTTGACCCGACGCGTATGACGCCAGCGGTGCGCCATAACGACGGGCTGGACTAT‑GTGCCGACGGACAAGAAAGTGCTGTTCGGTCA‑CCATTTTGCGGCCATTGCCGGAG‑CAGGTCCGCTGGTGGGGCCGGTAC‑TGGCGGCGCAAATGGGCTACCT‑GCCGGGGATGATCTGgct                                                                                                                                             <  1:323958/238‑1 (MQ=255)                                                                                                         tGTATCTATCTGATCGCTTACCGTTTTTATGGGCTGTATATCGCCAAAAATGTGCTGGCGGTTGACCCGACGCGTATGACGCCAGCGGTGCGCCATAACGACGGGCTGGACTAT‑GTGCCGACGGACAAGAAAGTGCTGTTCGGTCA‑CCATTTTGCGGCCATTGCCGGAG‑CAGGTCCGCTGGTGGGGCCGGTAC‑TGGCGGCGCAAATGGGCTACCT‑GCCGGGGATGATCTGGCTGCTCGc                                                                                                                                       <  1:650858/239‑1 (MQ=255)                                                                                                             tctatctGATCGCTTACCGTTTTTATGGGCTGTATATCGCCAAAAATGTGCTGGCGGTTGACCCGACGCGTATGACGCCAGCGGTGCGCCATAACGACGGGCTGGACTAT‑GTGCCGACGGACAAGAAAGTGCTGTTCGGTCA‑CCATTTTGCGGCCATTGCCGGAG‑CAGGTCCGCTGGTGGGGCCGGTAC‑TGGCGGCGCAAATGGGCTACCT‑GCCGGGGATGATCTGGCTGCTCGCTggg                                                                                                                                   <  1:856655/239‑1 (MQ=255)                                                                                                              ctatctGATCGCTTACCGTTTTTATGGGCTGTATATCGCCAAAAATGTGCTGGCGGTTGACCCGACGCGTATGACGCCAGCGGTGCGCCATAACGACGGGCTGGACTAT‑GTGCCGACGGACAAGAAAGTGCTGTTCGGTCA‑CCATTTTGCGGCCATTGCCGGAG‑CAGGTCCGCTGGTGGGGCCGGTAC‑TGGCGGCGCAAATGGGCTACCT‑GCCGGGGATGATCTGGCTGCTCGCTgggg                                                                                                                                  <  1:986262/239‑1 (MQ=255)                                                                                                                 tctGATCGCTTACCGTTTTTATGGGCTGTATATCGCCAAAAATGTGCTGGCGGTTGACCCGACGCGTATGACGCCAGCGGTGCGCCATAACGACGGGCTGGACTAT‑GTGCCGACGGACAAGAAAGTGCTGTTCGGTCA‑CCATTTTGCGGCCATTGCCGGAG‑CAGGTCCGCTGGTGGGGCCGGTAC‑TGGCGGCGCAAATGGGCTACCT‑GCCGGGGATGATCTGGGTGCTCGCTGgggggg                                                                                                                               >  1:201593/1‑239 (MQ=255)                                                                                                                     aTCGCTTACCGTTTTTATGGGCTGTATATCGCCAAAAATGTGCTGGCGGTTGACCCGACGCGTATGACGCCAGCGGTTCGCCATAACGACGGGCTGGAATAT‑GTGCCGACGGACAAGAAAGTGCTGTTCGGTCA‑CCATTTTGCGGCCATTGCCGGAG‑CAGGTCCGCTGGTGGGGCCGGTAC‑TGGCGGCGCAAAAGGGCTCACT‑GCCGGGGGTGATGGTGGTGCTCGCCGGGGGGGttt                                                                                                                            >  2:1084697/1‑237 (MQ=255)                                                                                                                     aTCGCTTACCGTTTTTATGGGCTGTATATCGCCAAAAATGTGCTGGCGGTTGACCCGACGCGTATGACGCCAGCGGTGCGCCATAACGACGGGCTGGACTAT‑GTGCCGACGGACCAGAAAGGGCTGTTCGGGCA‑CCATTTTGCGGCCATTGCCGGAG‑CAGGGCCGCTGGTGGGGCCGGGAC‑TGGCGGCGCCAATGGGGGACCT‑GCCGGGGGTGGGCTGGGTGCTCGCTGGGGGGGGtc                                                                                                                            >  2:919339/1‑238 (MQ=255)                                                                                                                     aTCGCTTACCGTTTTTATGGGCTGTATATCGCCAAAAATGTGCTGGCGGTTGACCCGACGCGGATGACGCCAGCGGTGCGCCATAACGACGGGCTGGACTAT‑GTGCCGACGGACAAGAAAGTGCTGTTCGGTCA‑CCCTTTTGCGGCCATTGCCGGAG‑CAGGTCCGTTGGTGGGGGCGGCAA‑TGGCGGGGAAAAAGGGGGACCT‑TCCGGGGTTGGTCTGGCGGCCTGCTGGGGGGGttt                                                                                                                            >  2:988875/1‑237 (MQ=255)                                                                                                                           tACCGTTTTTATGGGCTGTATATCGCCAAAAATGTGCTGGCGGTTGACCCGACGCGTATGACGCCAGCGGTGCGCCATAACGACGGGCTGGACTAT‑GTGCCGACGGACAAGAAAGTGCTGTTCGGTCA‑CCATTTTGCGGCCATTGCCGGAG‑CAGGTCCGCTGGTGGGGCCGGTAC‑TGGCGGCGCCAATGGGCTACCT‑GCCGGGGATGGTCTGGCTGCTCGCTGGGGTGGTTCTCG‑Ccgg                                                                                                                    >  1:1022519/1‑239 (MQ=255)                                                                                                                             ccGTTTTTATGGGCTGTATATCGCCAAAAATGTGCTGGCGGTTGACCCGACGCGTATGACGCCAGCGGTGCGCCATAACGACGGGCTGGACTAT‑GTGCCGACGGACAAGAAAGTGCTGTTCGGTCA‑CCATTTTGCGGCCATTGCCGGAG‑CAGGTCCGCTGGTGGGGCCGGTAC‑TGGCGGCGCAAATGGGCTACCT‑GCCGGGGATGATCTGGCTGCTCGCTGGGGTGGTTCTCG‑Ccggtg                                                                                                                  <  1:946912/239‑1 (MQ=255)                                                                                                                             ccGTTTTTATGGGCTGTATATCGCCAAAAATGTGCTGGCGGTTGACCCGACGCGTATGACGCCAGCGGTGCGCCATAACGACGGGCTGGACTAT‑GTGCCGACGGACAAGAAAGTGCTGTTCGGTCA‑CCATTTTGCGGCCATTGCCGGAG‑CAGGTCCGCTGGTGGGGCCGGTAC‑TGGCGGCGCAAATGGGCTACCT‑GCCGGGGATGATCTGGCTGCTCGCTGGGGTGGTTCTCG‑Ccggtg                                                                                                                  <  2:1160199/239‑1 (MQ=255)                                                                                                                             ccGTTTTTATGGGCTGTATATCGCCAAAAATGTGCTGGCGGTTGACCCGACGCGTATGACGCCAGCGGTGCGCCATAACGACGGGCTGGACTAT‑GTGCCGACGGACAAGAAAGTGCTGTTCGGTCA‑CCATTTTGCGGCCATTGCCGGAG‑CAGGTCCGCTGGTGGGGCCGGTAC‑TGGCGGCGCAAATGGGCTACCT‑GCCGGGGATGATCTGGCTGCTCGCTGGGGTGGTTCTCG‑Ccggtg                                                                                                                  <  1:750747/239‑1 (MQ=255)                                                                                                                             ccGCTTTTATGGGCTGTAGTCCGCCAAAAAGGTGCTGGCGGTTGGCCCGACGTGTAGGGCGCCAGCGGTGCGCCTTTCCGCCGGGCTGGACTAT‑GTGCCGACGGACAAGAAAGTGCTGTTGGGTCA‑CCATTTTGCGGCCATTGCCGGAG‑CAGGTCCGCTGGTGGGGCCGGTAC‑TGGCGGCGCAAATGGGCTACCT‑GCCGGGGGTGATCTGGCTGCTCGCTGGGGTGGTTCTCG‑Ccggtg                                                                                                                  <  2:717538/239‑1 (MQ=255)                                                                                                                               gTTTTTAGGGGCTGTATATCGCCAAAAATGTGCTGGCGGTTGACCCGACGCGTATGACGCCAGCGGTGCGCCATAACGACGGGCTGGACTAT‑GTGCCGACGGACAAGAAAGTGCTGTTCGGTCA‑CCATTTTGCGGCCATTGCCGGAG‑CAGGTCCGCTGGTGGGGCCGGTAC‑TGGCGGCGCAAATGGGCTACCT‑GCCGGGGATGATCTGGCTGCTCGCTGGGGTGGTTCTCG‑Ccggtgcg                                                                                                                <  2:407776/239‑1 (MQ=255)                                                                                                                                    tATGGGCTGTATATCGCCAAAAATGGGCTGGCGGTTGACCCGACGCGTATGACGCCAGCGGTGCGCCATAACGACGGGCTGGACTAT‑GTGCCGACGGACAAGAAAGTGCTGTTCGGTCA‑CCATTTTGCGGCCATTGCCGGAG‑CAGGTCCGCTGGTGGGGCCGGTAC‑TGGCGGCGCAAATGGGCTACCT‑GCCGGGGATGATCTGGCTGCTCGCTGGGGTGGTTCTCG‑CCGGTGCGGTGCa                                                                                                           <  1:988875/239‑1 (MQ=255)                                                                                                                                    tATGGGCGGTATATCGCCAAAAATGTGCTGGCGGTTGACCCGACGCGTATGACGCCAGCGGTGCGCCATAACGACGGGCTGGACTAT‑GTGCCGACGGACAAGAAAGTGCTGTTCGGTCA‑CCATTTTGCGGCCATTGCCGGAG‑CAGGTCCGCTGGTGGGGCCGGTAC‑TGGCGGCGCAAATGGGCTACCT‑GCCGGGGATGATCTGGCTGCTCGCTGGGGTGGTTCTCG‑CCGGTGCGGTGCa                                                                                                           <  1:657064/239‑1 (MQ=255)                                                                                                                                                 tCGCCAAAAATGTGCTGGCGGTTGGCCCGACGCGTATGACGCCAGCGGTGCGCCATAACGACGGGCTGGACTAT‑GTGCCGACGGACAAGAAAGTGCTGTTCGGTCA‑CCATTTTGCGGCCATTGCCGGAG‑CAGGTCCGCTGGTGGGGCCGGTAC‑TGGCGGCGCAAATGGGCTACCT‑GCCGGGGATGATCTGGCTGCTCGCTGGGGTGGTTCTCG‑CCGGTGCGGTGCAGGATTTCATGGTg                                                                                              <  1:561969/239‑1 (MQ=255)                                                                                                                                                  cGCCAAAAATGTGCTGGCGGTTGACCCGACGCGTATGACGCCAGCGGTGCGCCATAACGACGGGCTGGACTAT‑GTGCCGACGGACAAGAAAGTGCTGTTCGGTCA‑CCATTTTGCGGCCATTGCCGGAG‑CAGGTCCGCTGGTGGGGCCGGTAC‑TGGCGGCGCAAATGGGCTACCT‑GCCGGGGGTGGTCTGGCTGCTCGCTGGGGTGGGTCTCG‑CCGGGGCGGTGCAGGGTTTCATGGt                                                                                               >  1:886466/1‑237 (MQ=255)                                                                                                                                                  cGCCAAAAATGTGCTGGCGGTTGACCCGACGCGTATGACGCCAGCGGGGCGCCATAAAGACGGGCTGGACCAT‑GTGCCGACGGACAAGAAAGTGCTGTTCGGTCA‑CCATTTTGCGGCCATTGCCCGCG‑CAGGGCCGCTGGTGGGGCCGGGCA‑TGGCCGCGCAAATGGGGTACCT‑GCCGGGGGAGGTCTGGGTGCTCGTCGGGGGGGTTTTCG‑CCGGGGGGGTGGCGGGTTTCATGGGGc                                                                                             >  2:793401/1‑239 (MQ=255)                                                                                                                                                   gCCAAAAATGTGCTGGCGGTTGACCCGACGCGTATGACGCCAGCGGTGCGCCATAACGACGGGCTGGACTAT‑GTGCCGACGGACAAGAAAGTGCTGTTCGGTCA‑CCATTTTGCGGCCATTGCCGGAG‑CAGGTCCGCTGGTGGGGCCGGTAC‑TGGCGGCGCAAATGGGCTACCT‑GCCGGGGATGATCTGGCTGCTCGCTGGGGTGGTTCTCG‑CCGGTGCGGTGCAGGATTTCATGGTg                                                                                              <  1:1049730/237‑1 (MQ=255)                                                                                                                                                    ccAAAAATGTGCTGGCGGTTGACCCGACGCGTATGACGCCCGCGGTGCGCCATACCGCCGGGCTGGACTAT‑GTGCCGACGGACACGAAAGTGCTGTTCGGTCA‑CCATTTTGCGGCCATTGCCGGAA‑CAGGTCCCCTGGTGGGGCCGGTCC‑TGGCGGCCCACATGGGCTGCCT‑GCCGGGGGTGGTCTGGGTGCTCGTCGGGGGGGTGTTTG‑CCGGTGCGGTGGCGGCTTTTGTGGTGCtc                                                                                           >  2:649154/1‑238 (MQ=255)                                                                                                                                                       aaaaTGTGCTGGCGGTTGACCCGGCGCGTATGACGCCAGCGGGGGGCCATAACGACGGGCTGGACTAT‑TGGCCGACGGACAAGAAAGTGCTGTTCGGTCA‑CCATTTTGCGGCCATTGCCGGAG‑AAGGTCCGCTGGTGGGGCCGGTAC‑CGGCGGGGCAAAAGGGGCACCC‑GCCGGGGGGGGTGTGGGGGGTCGCCGGGGGGGTTTCCG‑GCGGGGCGGTGGCGGGTTTCAGGGGGCtggtt                                                                                        >  2:518732/1‑239 (MQ=255)                                                                                                                                                       aaaaTGTGCTGGCGGTTGACCCGACGCGTATGACGCCAGCGGTGCGCCATAACGACGGGCTGGACTAT‑GTGCCGACGGACAAGAAAGTGCTGTTCGGTCA‑CCATTTTGCGGCCATTGCCGGAG‑CAGGTCCGCTGGTGGGGCCGGTAC‑TGGCGGCGCAAATGGGCTACCT‑GCCGGGGGTGATCTGGGTGCTCGCTGGGGTGGTTCTTG‑CCGGGGCGGTGCAGGATTTCATGGGGCtggtt                                                                                        >  1:933859/1‑239 (MQ=255)                                                                                                                                                       aaaaTGTGCTGGCGGTTGACCCGACGCGTATGAAGCCAGCGGTGCGCCATCACGACGGGCTGGACTAT‑GTGCCGACGGACAAGAAAGGGCTGTTCGGGCA‑CCATTTTGCGGCCATTGTCGGAG‑CAGGTCCGCTGGTGGGGCCGGGAA‑TTGCGGGCCCAAAGGGGTCCCC‑GCCGGGGGGGATCTGGGGGATCGCCGGGGTGGTTGCCG‑CCGGGGCGGGGCCGGGTTTACTGGTGCtgttt                                                                                        >  2:573638/1‑239 (MQ=255)                                                                                                                                                               cTGGCGGTTGACCCGACGCGTATGACGCCAGCGGTGCCCCATAACGACGGGCTGGACTAT‑GTGCCGACGGACAAGAAAGTGCTGTTCGGTCA‑CCATTTTTCGGCCATTGCCGGAG‑CAGGTCCGGTGGTGGGGCCGGTAC‑TGGCGGCGCCAATGGGCGCCCC‑GCCGGGGGTGGTCTGGCTGCCTGCTCGGGGGGGTCGCGCCCGGTCGGGGGCGGGTTTTCTGGTTGTTgtttgtttgtc                                                                                 >  2:1187084/1‑236 (MQ=255)                                                                                                                                                                        gACCCGACGCGTATGACGCCAGCGGTGCGCCATAACGACGGGCTGGACTAT‑GTGCCGACGGACAAGAAAGTGCTGTTCGGTCA‑CCATTTTGCGGCCATTGCCGGAG‑CAGGTCCGCTGGTGGGGCCGGTAC‑TGGCGGCGCAAATGGGCTACCT‑GCCGGGGATGATCTGGCTGCTCGCTGGGGTGGTTCTCG‑CCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGa                                                                       <  1:1003964/239‑1 (MQ=255)                                                                                                                                                                            cGACGCGTATGACGCCAGCGGTGCGCCATAACGACGGGCTGGACTAT‑GTGCCGACGGACAAGAAAGTGCTGTTCGGTCA‑CCATTTTGCGGCCATTGCCGGAG‑CAGGTCCGCTGGTGGGGCCGGTAC‑TGGCGGCGCAAATGGGCTACCT‑GCCGGGGATGATCTGGCTGCTCGCTGGGGTGGTTCTCG‑CCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGt                                                                   <  1:831767/239‑1 (MQ=255)                                                                                                                                                                              aCGCGTATGACGCCAGCGGTGCGCCATAACGACGGGCTGGACTAT‑GTGCCGACGGACAAGAAAGTGCTGTTCGGTCA‑CCATTTTGCGGCCATTGCCGGAG‑CAGGTCCGCTGGTGGGGCCGGTAC‑TGGCGGCGCAAATGGGCTACCT‑GCCGGGGATGATCTGGCTGCTCGCTGGGGTGGTTCTCG‑CCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGtc                                                                  >  1:778960/1‑238 (MQ=255)                                                                                                                                                                                gcgTATGACGCCGGCGGTGCGCCATAACGACGGGCTGGACTAT‑GTGCCGACGGACAAGAAAGTGCTGTTCGGTCA‑CCATTTTGCGGCCATTGCCGGAG‑CAGGTCCGCTGGTGGGGCCGGTAC‑TGGCGGCGCAAATGGGCTACCT‑GCCGGGGATGATCTGGCTGCTCGCTGGGGTGGTTCTCG‑CCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGtcgct                                                               <  1:681039/239‑1 (MQ=255)                                                                                                                                                                                gcgTATGACGCCAGCGGTGCGCCATAACGACGGGCTGGACTAT‑GTGCCGACGGACAAGAAAGTGCTGTTCGGTCA‑CCATTTTGCGGCCATTGCCGGAG‑CAGGTCCGCTGGTGGGGCCGGTAC‑TGGCGGCGCAAATGGGCTACCT‑GCCGGGGATGATCTGGCTGCTCGCTGGGGTGGTTCTCG‑CCGGTGCGGTGCAGGATTTCCTGGTGCTGTTTGGTTCTACGCGCCGTGCAGGtcgc                                                                >  2:485531/1‑238 (MQ=255)                                                                                                                                                                                  ggaTGACGCAGGGGGGGCGCCCTAACGGCGGGCGGGACTGT‑GGGCCGACGGAGAAGAAAGGGCTGTTCGGTCA‑CCATTTTGCGGCGATGGCCGGAG‑CAGGGCCGCTGGGGGGGCCGGTTC‑GGGCGGCGCAAGTGGGCTACCT‑GCCGGGGATGATCTGGCTGCTCGCTGGGGTGGTTCTCG‑CCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGtcgctc                                                              <  2:627239/236‑1 (MQ=255)                                                                                                                                                                                  gTATGACGCCAGCGGTGCGCCATAACGACGGGCTGGACTAT‑GTGCCGACGGACAAGAAAGTGCTGTTCGGTCA‑CCATTTTGCGGCCATGGCCGGAG‑CAGGTCCGCTGGTGGGGCCGGTAC‑TGGCGGCGCAAATGGGCTACCT‑GCCGGGGATGATCTGGCTGCTCGCTGGGGTGGTTCTCG‑CCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGtcgctcg                                                             <  1:432149/239‑1 (MQ=255)                                                                                                                                                                                    aTGACGCAATGGGTGCGCCAGAACGACGGGCTGGACTAT‑GTGCCGACGGACAAGAAAGTGCTGTTCGGTCG‑CCATTTTGCGGCCATTGCCGGAG‑CAGGTCCGCTGGTGGGGCCGGTAC‑TGGCGGCGCAAATGGGCTACCT‑GCCGGGGATGATCTGGCTGCTCGCTGGGGTGGTTCTCG‑CCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTcgctcgct                                                           <  2:1068206/239‑1 (MQ=255)                                                                                                                                                                                       gcggcAGCGGGGGGCCATAACGGCGGGCGTGACTAT‑GGGCCGGCGGGCAAGAAAGTGCGGTTCGGTCA‑CCATTTTGCGGCCATTGCCGGAG‑CAGGTCCGCTGGTGGGGCCGGTAC‑TGGCGGCGCAAATGGGCTACCT‑GCCGGGGATGATCTGGCTGCTCGCTGGGGTGGTTCTCG‑CCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGCTCGCTgg                                                         <  2:524906/234‑1 (MQ=255)                                                                                                                                                                                           cAGCGGTGCGCCATAGCGACGGGCTGGTGTAG‑GTGCCGACGGACAAGAAAGTGCTGTTCGGTCA‑CCATTTTGCGGCCATTGCCGGAG‑CAGGTCCGCTGGTGGGGCCGGTAC‑TGGCGGCGCAAATGGGCTACCT‑GCCGGGGATGATCTGGCTGCTCGCTGGGGTGGTTCTCG‑CCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGCTCGCTGGGTGAg                                                    <  2:96096/239‑1 (MQ=255)                                                                                                                                                                                           cAGCGGTGCGCCATAACGACGGGCTGGACTAT‑GTGCCGACGGACAAGAAAGTGCTGTTCGGTCA‑CCATTTTGCGGCCATTGCCGGAG‑CAGGTCCGCTGGTGGGGCCGGTAC‑TGGCGGCGCAAATGGGCTACCT‑GCCGGGGATGATCTGGCTGCTCGCTGGGGTGGTTCTCG‑CCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGCTCGCTGGGTGAg                                                    <  1:894421/239‑1 (MQ=255)                                                                                                                                                                                                   cgcCATAACGACGGGCTGGACTAT‑GTGCCGACGGACAAGAAAGTGCTGTTCGGTCA‑CCATTTTGCGGCCATTGCCGGAG‑CAGGTCCGCTGGTGGGGCCGGTAC‑TGGCGGCGCAAATGGGCTACCT‑GCCGGGGATGATCTGGCTGCTCGCTGGGGTGGTTCTCG‑CCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGGCGGTCGCTCGCTGGGTGGGCTGGTCag                                            >  2:634647/1‑238 (MQ=255)                                                                                                                                                                                                   cgcCATAACGACGGGCTGGACTAT‑GTGCCGACGGACAAGAAAGTGCTGTTCGGTCA‑CCATTTTGCGGCCATTGCCGGAG‑CAGGTCCGCTGGTGGGGCCGGTAC‑TGGCGGCGCAAATGGGCTACCT‑GCCGGGGATGATCTGGCTGCTCGCTGGGGTGGGTCTCG‑CCGGTGCGGTGCAGGATTTCCTGGTGCTGTCTGTTTCTACGCGCCGTGACGGTCGCTCGCTGGGTGGGCTGGTCaa                                            >  1:1037068/1‑239 (MQ=255)                                                                                                                                                                                                   cgcCATAACGAAGGGCTGGACTAT‑GTGCCGACGGACAAGAAAGTGCTGTTCGGTCA‑CCATTTTGCGGCCATTGCCGGAG‑CAGGTCCGCTGGTGGGGCCGGTAC‑TGGCGGCGCAAATGGGCTACCT‑GCCGGGGATGATCTGGCTGCTCGCTGGGGTGGTTCTCG‑CCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGCTCGCTGGGTGAGCTGGTCa                                             <  2:671047/238‑1 (MQ=255)                                                                                                                                                                                                      cttAACGCGGGGCTGGACTGT‑GTGCCGGCGGACAAGAAAGGTCTGTTGGGTCC‑CCATTTGGCGGCCATTGCGGGAG‑CAGGTCCGCTGGTGGGGCCGGTAC‑TGGCGGCGCAAATGGGCTCCCT‑GCCGGGGATGATCTGGCTGCTCGCTGGGGTGGTTCTCG‑CCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGCTCGCTGGGTGAGCTGGTCAaag                                          <  2:446132/236‑1 (MQ=255)                                                                                                                                                                                                                ggCTGGACTAT‑GTGCCGACGGACAAGAAAGTGCTGTTCGGTCA‑CCATTTTGCGGCCATTGCCGGAG‑CAGGTCCGCTGGTGGGGCCGGTAC‑TGGCGGCGCAAATGGGCTACCT‑GCCGGGGATGATCTGGCTGCTCGCTGGGGTGGTTCTCG‑CCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGCTCGCTGGGTGAGCTGGTCAAAGAAGAGATggg                                >  1:527090/1‑238 (MQ=255)                                                                                                                                                                                                                   tGGACTGG‑GTGCGGACGGACAGGAGAGTGCGGTGCGGTCA‑GTGTTTGGGCGCCATGTCGGGCT‑CGGGGGGGCTGGTGGGCTGGGTGC‑TGGCGGCGCAATTGGGGTACCT‑GCCGGGGATGGTTGGGCTGCTCTTTGTGTTGGTTCTCG‑CCGGTGCGGGGCAGGATTTCTGGGTGCTGTTTGTTTCTACGCGCCGTGACGGCCGCTCGGGGGGTGAGCTGGTCAAAGAAGAGGTGGGGcc                             <  2:616945/238‑1 (MQ=255)                                                                                                                                                                                                                   tGGACTAT‑GTGCCGACGGACAAGAAAGTGCTGTTCGGTCA‑CCATTTTGCGGCCATTGCCGGAG‑CAGGTCCGCTGGTGGGGCCGGTAC‑TGGCGGCGCAAATGGGCTACCT‑GCCGGGGATGATCTGGCTGCTCGCTGGGGTGGTTCTCG‑CCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGCTCGCTGGGTGAGCTGGTCAAAGAAGAGATGGGGcc                             <  1:862695/238‑1 (MQ=255)                                                                                                                                                                                                                        tgt‑gtgCCGAGGGACAAGAAAGGGCGGTTGGGTCA‑CCATTTTGCGGCCATTGCCGGGG‑CAGGTCCGCTGGTGGGGCCGGTAC‑GGGCGGCGCAAAGGGGCTCCCT‑GCCGGGGATGATCTGGCTGCTCGCTGGGGTGGTTCTCG‑CCGGTGCGGTGCAGGATTTCATGGTGCGGTTTGTTTCGACGCGCCGTGACGGTCGCTCGCTGGGGGAGCTGGTCAAAGAAGAGATGGGGCCAAccgc                       <  2:19882/237‑1 (MQ=255)                                                                                                                                                                                                                          t‑gtgCCGACGGACAAGAAAGTGCTGTTCGGTCA‑CCATTTTGCGGCCATTGCCGGAG‑CAGGTCCGCTGGTGGGGCCGGTAC‑TGGCGGCGCAAATGGGCTACCT‑GCCGGGGATGATCTGGCTGCTCGCTGGGGTGGTTCTCG‑CCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGCTCGCTGGGTGAGCTGGTCAAAGAAGAGATGGGGCCAAccgccg                     >  1:317430/1‑239 (MQ=255)                                                                                                                                                                                                                          t‑gtgCCGACGGACAAGAAAGTGCTGTTCGGTCA‑CCATTTTGCGGCCATTGCCGGAG‑CAGGTCCGCTGGTGGGGCCGGTAC‑TGGCGGCGCAAATGGGCTACCT‑GCCGGGGATGATCTGGCTGCTCGCTGGGGTGGTTCTCG‑CCGGGGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGCTCGCTGGGTGAGCTGGTCAAAGAAGAGATGGGGCCAAccgccg                     >  1:849610/1‑239 (MQ=255)                                                                                                                                                                                                                                cGACGGACAAGAAAGTGCTGTTCGGTCA‑CCATTTTGCGGCCATTGCCGGAG‑CAGGTCCGCTGGTGGGGCCGGTAC‑TGGCGGCGCAAATGGGCTACCT‑GCCGGGGATGATCTGGCTGCTCGGTGGGGTGGTTCTCG‑CAGGTGCGGAGCAGGATTTGATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGCTCGCTGGGTGAGCTGGTCAAAGAAGAGATGGGGCCAACCGCCGGGGTg                >  1:1081764/1‑239 (MQ=255)                                                                                                                                                                                                                                cGACGGACAAGAAAGTGCTGTTCGGTCA‑CCATTTTGCGGCCATTGCCGGAG‑CAGGTCCGCTGGTGGGGCCGGTAC‑TGGCGGCGCAAATGGGCTACCT‑GCCGGGGATGATCTGGCTGCTCGCTGGGGTGGTTCTCG‑CCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGCTCGCTGGGTGAGCTGGTCAAAGAAGAGATGGGGCCAACCGCCgggggg                >  1:919019/1‑237 (MQ=255)                                                                                                                                                                                                                                cGACGGACAAGAAAGTGCTGTTCGGTCA‑CCATTTTGCGGCCATTGCCGGAG‑CAGGTCCGCTGGTGGGGCCGGTAC‑TGGCGGCGCAAATGGGCTACCT‑GCCGGGGATGATCTGGCTGCTCGCTGGGGTGGTTCTCG‑CCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGCTCGCTGGGTGAGCTGGTCAAAGAAGAGATGGGGCCAACCGCCGGGGTg                >  1:80787/1‑239 (MQ=255)                                                                                                                                                                                                                                     gACAAGAAAGTGCTGTTCGGTCA‑CCATTTTGCGGCCATTGCCGGAG‑CAGGTCCGCTGGTGGGGCCGGTAC‑TGGCGGCGCAAATGGGCTACCT‑GCCGGGGATGATCTGGCTGCTCGCTGGGGAGGTTCTCG‑CCGGTGCGGTGCAGGATTTGATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGCTCGCTGGGTGAGCTGGTTCACGACGAGATGGCGCCAACCGCCAGGGCGATTgc           >  2:156303/1‑239 (MQ=255)                                                                                                                                                                                                                                     gACAAGAAAGTGCTGTTCGGTCA‑CCATTTTGCGGCCATTGCCGGAG‑CAGGTCCGCTGGTGGGGCCGGTAC‑TGGCGGCGCAAATGGGCTACCG‑GCCGGGGATGATCTGGCTGCTCGCTGGGGTGGTTCTCG‑ACGGTGCGGGGCAGGATTTCATGGTGCTGTTTGTTGCTACGCGCCGTGACGGTCGCTCGCTGGGTGATCTGGTATATGAAGAGGTGGGGGCAGCAGCGGGGGTGATTgc           >  2:326474/1‑239 (MQ=255)                                                                                                                                                                                                                                             aGTGCTGTTCGGTCA‑CCATTTTGCGGCCATTGCCGGAG‑CAGGTCCGCTGGTGGGGCCGGTAC‑TGGCGGCGCAAATGGGCTACCT‑GCCGGGGATGATCTGGCTGCTCGCTGGGGTGGTTCTCG‑CCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGCTCGCTGGGTGAGCTGGTCAAAGAAGAGATGGGGCCAACCGCCGGGGTGATTGCGCtggtg    >  1:1120241/1‑238 (MQ=255)                                                                                                                                                                                                                                               tGCTGTTCGGTCA‑CCATTTTGCGTCCATTGCCGGCG‑CAGGGCGTGTGGTGGGGCCGGTAC‑TGGGGGCGCAAATGGGCTACCT‑GCCGGGGATGATCTGGCTGCTGGCTGGGGTGGTTCTCG‑CCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGGGACGGTCGCTCGCTGGGTGAGCTGGTCAAAGAAGAGATGGGGCCAACCGCCGGGGTGATTGCGCTGGTGGc  <  2:969417/238‑1 (MQ=255)                                                                                                                                                                                                                                               tGCTGTTCGGTCA‑CCATTTTGCGGCCATGGCCGGAG‑CAGGTCCGCTGGTGGGGCCGGTAC‑TGGCGGCGCAAATGGGCTACCT‑GCCGGGGATGATCTGGCTGCTCGCTGGGGTGGTTCTCG‑CCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGCTCGCTGGGTGAGCTGGTCAAAGAAGAGATGGGGCCAACCGCCGGGGTGATTGCGCTGGTGGc  <  1:1187084/238‑1 (MQ=255)                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                   TCGTCTGGACAGTGCTCTCTGTAATGGGAGCATTTGCTCTGGGATACATTGCTTTAAATCGTGGGGAACAGATCAACGCGCTGTGGATTGTGGTGGCGTCGGTCTGTATCTATCTGATCGCTTACCGTTTTTATGGGCTGTATATCGCCAAAAATGTGCTGGCGGTTGACCCGACGCGTATGACGCCAGCGGTGCGCCATAACGACGGGCTGGACTAT‑GTGCCGACGGACAAGAAAGTGCTGTTCGGTCA‑CCATTTTGCGGCCATTGCCGGAG‑CAGGTCCGCTGGTGGGGCCGGTAC‑TGGCGGCGCAAATGGGCTACCT‑GCCGGGGATGATCTGGCTGCTCGCTGGGGTGGTTCTCG‑CCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGCTCGCTGGGTGAGCTGGTCAAAGAAGAGATGGGGCCAACCGCCGGGGTGATTGCGCTGGTGGC  >  NC_000913/629916‑630390

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑