mutation predictions | marginal predictions | summary statistics | genome diff | command line log
|
breseq version 0.35.4 revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log |
| read file | reads | bases | passed filters | average | longest | mapped | |
|---|---|---|---|---|---|---|---|
| errors | pgi-8-3_S15_L001_R1_001.good.fq | 2,743,805 | 632,211,355 | 100.0% | 230.4 bases | 289 bases | 99.3% |
| errors | pgi-8-3_S15_L001_R2_001.good.fq | 2,743,805 | 634,751,863 | 100.0% | 231.3 bases | 289 bases | 95.0% |
| total | 5,487,610 | 1,266,963,218 | 100.0% | 230.9 bases | 289 bases | 97.2% |
| seq id | length | fit mean | fit dispersion | % mapped reads | description | ||
|---|---|---|---|---|---|---|---|
| coverage | distribution | NC_000913 | 4,641,652 | 265.8 | 10.5 | 100.0% | Escherichia coli str. K-12 substr. MG1655, complete genome. |
| total | 4,641,652 | 100.0% |
fit dispersion is the ratio of the variance to the mean for the negative binomial fit. It is =1 for Poisson and >1 for over-dispersed data.
| option | limit | actual |
|---|---|---|
| Number of alignment pairs examined for constructing junction candidates | ≤ 100000 | 18390 |
| Coverage evenness (position-hash) score of junction candidates | ≥ 2 | ≥ 3 |
| Test this many junction candidates (n). May be smaller if not enough passed the coverage evenness threshold | 100 ≤ n ≤ 5000 | 65 |
| Total length of all junction candidates (factor times the reference genome length) | ≤ 0.1 | 0.008 |
| reference sequence | pr(no read start) |
|---|---|
| NC_000913 | 0.72088 |
pr(no read start) is the probability that there will not be an aligned read whose first base matches a given position on a given strand.
| option | value |
|---|---|
| Coverage evenness (position-hash) score of predicted junctions must be | ≥ 3 |
| Minimum probablilty assigned that no mapped read will start at a given position and strand for junction prediction | 0.1 |
| Junction allow suboptimal matches | FALSE |
| Skew score of predicted junction (−log10 probability of unusual coverage evenness) must be | ≤ 0 |
| Number of bases that at least one read must overlap each uniquely aligned side of a predicted junction | ≥ 6 |
| option | value |
|---|---|
| Mode | Full Polymorphism |
| Ploidy | 1 (haploid) |
| Consensus mutation E-value cutoff | 10 |
| Consensus frequency cutoff | 0.75 |
| Consensus minimum variant coverage each strand | OFF |
| Consensus minimum total coverage each strand | OFF |
| Consensus minimum variant coverage | OFF |
| Consensus minimum total coverage | OFF |
| Polymorphism E-value cutoff | 2 |
| Polymorphism frequency cutoff | 0.05 |
| Polymorphism minimum variant coverage each strand | 2 |
| Polymorphism minimum total coverage each strand | OFF |
| Polymorphism minimum variant coverage | OFF |
| Polymorphism minimum total coverage | OFF |
| Polymorphism bias cutoff | OFF |
| Predict indel polymorphisms | YES |
| Skip indel polymorphisms in homopolymers runs of | ≥3 bases |
| Skip base substitutions when they create a homopolymer flanked on each side by | ≥5 bases |
| program | version |
|---|---|
| bowtie2 | 2.2.6 |
| R | 3.4.4 |
| step | start | end | elapsed |
|---|---|---|---|
| Read and reference sequence file input | 10:42:04 06 Apr 2022 | 10:43:57 06 Apr 2022 | 1 minute 53 seconds |
| Read alignment to reference genome | 10:43:58 06 Apr 2022 | 11:04:03 06 Apr 2022 | 20 minutes 5 seconds |
| Preprocessing alignments for candidate junction identification | 11:04:03 06 Apr 2022 | 11:05:33 06 Apr 2022 | 1 minute 30 seconds |
| Preliminary analysis of coverage distribution | 11:05:33 06 Apr 2022 | 11:10:33 06 Apr 2022 | 5 minutes 0 seconds |
| Identifying junction candidates | 11:10:33 06 Apr 2022 | 11:11:26 06 Apr 2022 | 53 seconds |
| Re-alignment to junction candidates | 11:11:26 06 Apr 2022 | 11:15:33 06 Apr 2022 | 4 minutes 7 seconds |
| Resolving best read alignments | 11:15:33 06 Apr 2022 | 11:17:48 06 Apr 2022 | 2 minutes 15 seconds |
| Creating BAM files | 11:17:48 06 Apr 2022 | 11:21:58 06 Apr 2022 | 4 minutes 10 seconds |
| Tabulating error counts | 11:21:58 06 Apr 2022 | 11:24:06 06 Apr 2022 | 2 minutes 8 seconds |
| Re-calibrating base error rates | 11:24:06 06 Apr 2022 | 11:24:07 06 Apr 2022 | 1 second |
| Examining read alignment evidence | 11:24:07 06 Apr 2022 | 14:25:02 06 Apr 2022 | 3 hours 55 seconds |
| Polymorphism statistics | 14:25:02 06 Apr 2022 | 14:25:03 06 Apr 2022 | 1 second |
| Output | 14:25:03 06 Apr 2022 | 14:25:31 06 Apr 2022 | 28 seconds |
| Total | 3 hours 43 minutes 26 seconds | ||
